Anode Chamber Research Articles

Engineering extracellular electron transfer to promote simultaneous brewing wastewater treatment and chromium reduction

Microbial fuel cell (MFC) technology has been developed for wastewater treatment in the anodic chamber, and heavy metal reduction in the cathodic chamber. However, the limited extracellular electron transfer (EET) rate of exoelectrogens remained a constraint for practical applications of MFCs. Here, a MFC system that used the electricity derived from anodic wastewater treatment to drive cathodic Cr6+ reduction was developed, which enabled an energy self-sustained approach to efficiently address Cr6+ contamination. This MFC system was achieved by screening exoelectrogens with a superior EET rate, promoting the exoelectrogenic EET rate, and constructing a conductive bio-anode. Firstly, Shewanella algae-L3 was screened from brewing wastewater acclimatized sludge, which generated power density of 566.83 mW m−2. Secondly, to facilitate EET rate, flavin synthesis gene operon ribADEHC was overexpressed in engineered S. algae-L3F to increase flavins biosynthesis, which promoted the power density to 1233.21 mW m−2. Thirdly, to facilitate interface electron transfer, carbon nanotube (CNT) was employed to construct a S. algae-L3F-CNT bio-anode, which further enhanced power density to 3112.98 mW m−2. Lastly, S. algae-L3F-CNT bio-anode was used to harvest electrical energy from brewing wastewater to drive cathodic Cr6+ reduction in MFC, realizing 71.43% anodic COD removal and 98.14% cathodic Cr6+ reduction. This study demonstrated that enhanced exoelectrogenic EET could facilitate cathodic Cr6+ reduction in MFC.

Anodic biodegradation of phenol in a microbial desalination cell through microbial composition shift for energy recovery and water desalination

This study assessed the performance of a three-chamber microbial desalination cell (MDC) for simultaneous electricity generation, phenol biodegradation, and salt removal from water. The MDC operated with anaerobic sludge in the anode chamber at varying phenol concentrations in the range of 100–1000 mg L−1 and bio-cathode with sodium acetate as the catholyte. The highest power density (7.5 W m−3), desalination efficiency (66.9 %), and current efficiency (75.5 %) were obtained at optimal phenol concentration of 700 mg L−1. Non-electroactive bacteria dominated the anode microbial community through high-throughput sequencing analysis. The population of electroactive bacteria decreased, including Geobacter and Rhodopseudomonas from 9.8 % and 7.8 % to 0.9 % and 0.6 %, respectively, due to phenol toxicity, Cl− migration, and pH fluctuations. Meanwhile, other genera, such as Chryseobacterium (from 0.8 % to 4.5 %) and Clostridium (from 1.4 % to 2.2 %), were observed to thrive in the saline and acidic phenolic environment of the anode chamber.

Na+ migration facilitated separation of hydrogen and hydroxide for efficient hardness removal via an integrated electrochemical precipitation approach

In this study, efficient H+–OH– separation was achieved employing Na+ migration in anode chamber in a cation exchange membrane assembled electrochemical system for enhanced hardness removal. It was found that the OH– current efficiency could reach 91.3%, when hydraulic residence time (HRT) of anode chamber and the electrode distance were 1 min and 1 cm, respectively. Then, most of the retained OH– would be transported to a separated crystallizer to react with the feed water to perform hardness removal. Besides, Na+ balance could be maintained using accumulated Na+ in the crystallizer to backfill the anode circulator, so as to strengthen the current efficiency of OH– of the cathode chamber throughout the process. Specifically, at a current density and an inflow rate of 10 mA/cm2 and of 20 mL/min, hardness removal efficiency and the energy consumption reached 92.1% and 2.26 kWh/kg CaCO3, respectively. Moreover, it was found that large specific surface area of the carbon felt adopted for precipitation and crystallization could be recycled up to 15 repeated cycles (about 180 h) in terms of the pure water permeance. Finally, aragonite was characterized to be dominant at the reaction, whereas magnesium calcite could also be observed at the end of the reaction process (12 h). In conclusion, Na+ migration as well as carbon felt dependent precipitation impelled integrated electrochemical system would increase hardness removal efficiency sustainably.

Relationship between organic removal and power production in a dual-chamber microbial fuel cell with intermittent mode

Unoptimised simultaneous performance in microbial fuel cell (MFC) is still a big concern due to a lack of information on the correlation between organic removal and power production. Its correlation becomes more substantial owing to the main factors which affect a concurrent condition. To contribute new insight, this study aimed to analyze the relationship between the main factors for determining the optimal condition of MFC performance. Dual-chamber MFC (DCMFC) was designed by modifying the anode chamber into two compartments, namely double anode chamber DCMFC (DAC-DCMFC), operated within 8 days running with intermittent mode. The differences of organic loading rate (OLR), 0.4; 1.0; 2.5 kg.m−3.d−1 represented low to high organic loadings and electrode material-based reactor types, were used to assign the optimal concomitant performance in DCMFC. A closed circuit voltage (CCV) wiring system plugged onto the data logger within running time was employed to evaluate the synchronous achievement. This study result was medium OLR 1.0 kg.m−3.d−1, and GNPs anode-PTFE cathode attained optimally in the performance. In addition, higher OLR does not indicate higher organic removal correlating linearly with power production. This finding contributes to the limitation of organic loading that biological role capabilities can use.

Production of bioelectricity from palm oil mill effluent through a dual chamber-microbial fuel cell system with the addition of Lactobacillus bulgaricus

Palm oil liquid waste has been successfully developed to produce bio-electricity with a dual chamber-microbial fuel cell system. This study utilized the Lactobacillus bulgaricus bacteria as a support for the substrate samples prepared in the anode chamber. Meanwhile, in the cathode chamber, KMnO4 electrolyte solution is used as an electroactive species that can capture electrons well. In addition, salt bridges fabricated from agar have a role as ion-exchange media in microbial fuel cells. The test results showed that the best performance was obtained in samples of palm oil wastewater with the addition of 10% Lactobacillus bulgaricus (LS/B-10) bacteria with current, voltage, and power density values of 0.9640 mA, 0.6760 V, and 248.04 mW/m2, respectively. The MFC system has also been proven to be able to reduce COD (Chemical Oxygen Demand) and TSS (Total Suspended Solid) levels, with the results of a reduction percentage of 42.6% and 7.2%, respectively, in the LS/B-10 variable treatment. All test results show that palm oil wastewater with the addition of Lactobacillus bulgaricus bacteria is promising for producing bioelectricity with a microbial fuel cell system.

Novel three-dimensional electrode structures with urchin-like pore canal for high-performance microbial fuel cells

In this work, a kind of urchin-like porous structural gel beads as three-dimensional (3D) packed anode materials were prepared to utilize the chemical coprecipitation method, forming the reduced graphene/chitosan/Fe3O4 (rGO/CHI/Fe3O4) gel beads (GFe100). The average diameter of Fe3O4 nanoparticles in the gel beads was about 13 nm, distributing uniformly in GFe100 gel beads in situ. Fe3O4 nanoparticles formed in the material-preparation process had a significant pore-creating function to form urchin-like pore canals with the help of the micro-reactor. The average size of these canals was 13.55 μm, which could enhance more functional microbes in the gel beads to strengthen the biocompatibility and extracellular electron transfer (EET) of anode electrodes. The maximum output voltage and power density of GFe100 was 759 mV and 17.823 W·m-3, which was 2.3 times (327 mV) and 23.21 times (0.768 W m-3) higher than that of GFe0 prepared without any addition of Fe2+, respectively. The significant electricity generation performance of GFe100 was mainly attributed to the urchin-like canal structure. Exoelectrogens possessed the macropore structure for the controlled diffusion process and extracellular metabolic products occupied the micropore and mesopore structure donating for the surface reactant process. This work suggested that three-dimensional gel beads filled into the anode chamber might be the potential for long-term MFC practice application with a high electricity generation performance.

Selection of the most suitable pretreatment method by PROMETHEE for baker's yeast production wastewater in Microbial Fuel Cell

AbstractBACKGROUNDThe high organic load and low biodegradability of the effluent from baker's yeast production limit its use as a substrate in microbial fuel cell (MFC) systems. In this study, baker's yeast production wastewater was used as a substrate in the anode chamber to assess the impact of various pretreatment methods [dilution, microwave (MW), acidic‐thermal, basic‐thermal and ultrasound (US)] on electricity generation and waste treatment. Additionally, azo dye was used as an electron acceptor at the cathode. Power density and coulomb efficiency were evaluated for electricity generation, and chemical oxygen demand (COD) removal and azo dye removal parameters were evaluated for treatability.RESULTSThe maximum power densities obtained were 83.53, 46.54, 19.41, 67.40, 41 and 33 mW m−2 for MW, acidic thermal, alkaline‐thermal and US pretreatments, diluted raw and raw wastewater, respectively. To determine the most suitable pretreatment method, PROMETHEE was performed using the criteria of maximum power density, COD removal, azo dye removal, coulombic efficiency and pretreatment cost. The most suitable method was found to be MW pretreatment. The order of the alternatives was found to be MW > US > acidic/thermal > diluted raw > raw > alkaline/thermal. With the MW pretreatment, 81.34% azo dye removal, 5 g L−1 COD removal, 0.27% coulombic efficiency and €0.284 L−1 pretreatment cost were obtained.CONCLUSIONThe most suitable pretreatment method to be applied to wastewater for the treatment of baker's yeast production wastewater and high energy production was determined as MW using the PROMETHEE approach. © 2023 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).

The Effect of Nutrients in Anodic Chamber to the Performance of Microbial Fuel Cell (MFC)

This paper describes a device known as a Single-chamber Microbial Fuel Cell (SMFC) that was used to generate bioelectricity from plant waste containing lignocellulosic components, such as bamboo leaves, rice husk and coconut waste, with various anodic chamber substrate compositions. The maximum power density among all assembled SMFCs was determined to be 231.18 mW/m2, generated by coconut waste. This model’s bioelectricity production was enhanced by adding organic compost to the anodic chamber, which acts as a catalyst in the system. The maximum power density of 788.58 mW/m2 was attained using a high proportion of coconut waste (CW) and organic compost. These results show that the higher percentage of lignin in CW improved the bioelectricity of SMFC.

Sustainable in situ ammonia recovery from municipal solid waste leachate in a single-stream microbial desalination cell

Municipal solid waste (MSW) leachate is one of the most hazardous waste streams leading to great potential risk to environment, and a renewable resource with high concentrations of organic contaminant and ammonia. High energy consumption and chemical input are still the challenges for ammonia recovery from MSW leachate. Here, a single-stream microbial desalination cell (SMDC) was successfully developed for simultaneous energy extraction from organic contaminant and in-situ energy utilization for ammonia recovery. 70% of the organic contaminant from the actual MSW leachate was removed, and 24.9% of the total ammonia was recovered as high-purity (NH4)2SO4. The additional desalination chamber introduced into the SMDC can potentially enhance the NH4+ migration that was determined by the NH4+ concentration gradient and electric field. More than 30% of the total nitrogen was lost, as revealed by nitrogen mass balance analysis, probably resulting from the anodic denitrification process driven by denitrifying microorganisms, e.g., Thauera, which thrived in the anode chamber. Concomitantly, the chemical input for ammonia stripping can be reduced by up to 68% due to the relatively low buffer capacity of the catholyte and the OH− production from the cathode reaction. This SMDC can be an effective and environmentally sustainable solution for MSW leachate treatment and resource recovery.

Enhancing microbial desalination cell performance for water desalination and wastewater treatment: experimental study and modelling of electrical energy production in open and closed‐circuit modes

AbstractBACKGROUNDMicrobial desalination cell (MDC) is a new technology in the use of electrical energy for water desalination and wastewater treatment.RESULTSOpen circuit (OC) and closed circuit (CC) modes were successfully simulated with initial TDS concentrations of 10 g/L and 10–15 g/L, respectively (an external resistance of only 150 Ω was applied for CC). After 160 h of operation, the maximum OC voltage, desalination efficiency and COD removal efficiency were 809 mV, 32.2% and 79.2%, respectively. The maximum voltage was also obtained when the external resistances were 150 Ω (423.4 and 438 mV) for the initial NaCl concentrations (10 and 15 g/L) in the central chamber, respectively. Moreover, the maximal desalting and COD removal efficiencies after 24 h run‐time were (30% and 28%) and (24% and 25%) for initial NaCl concentrations (10 or 15 g/L) in the central chamber, respectively. Maintaining pH (8.61, 7.01) to (7.85, 7.8) in anode and cathode chambers was studied. This research accurately depicted microbial desalination's efficiency in generating OC voltages and CC electrical energy via Box–Behnken Design Distribution (BBD). Investigated factors’ interactions (initial salt/COD concentrations, time) on CC system's energy efficiency. Resulted in peak productivity at (1.85 mW), OC reaching (1100 mV).CONCLUSIONFinally, the research paper gave exciting results in improving the efficiency of the microbial desalination cell to increase the ability to produce electrical energy and desalinate water. © 2023 Society of Chemical Industry (SCI).

Reduced graphene oxide-supported palladium oxide-MOx for improving the performance of air-cathode microbial fuel cells: Influence of the Sn, Ce, Zn, and Fe precursors

Over the past few decades, microbial fuel cells (MFCs) have attracted significant interest within the realm of renewable energy technologies owing to their capability to generate electricity from low-grade substrates. However, their commercialization and scaling-up are impeded by significant obstacles, including high capital costs, limited durability, and sluggish oxygen reduction reaction (ORR). Here, we demonstrate an environmentally-friendly approach for developing highly efficient electrocatalysts consisting of palladium oxide (PdO) and different transition metal oxides (i.e., tin (IV) oxide (SnO2), ceric oxide (CeO2), iron (III) oxide (Fe2O3), and zinc oxide (ZnO)) embedded within reduced graphene oxide (rGO) framework for improving the efficiency of ORR in neutral medium and MFCs. Among the as-prepared electrocatalysts, the PdO–SnO2/rGO possesses the highest ORR electrochemical catalytic activity in a neutral medium (pH ∼ 7.2) following a four-electron ORR pathway, with a current density slightly lower than that of benchmark Pt/C electrocatalyst. The assessment of MFC performance is conducted by employing activated sludge as the inoculum and glucose as the sole electron donor in the anode chamber of single-chamber, air-cathode MFCs. Consistent with electrochemical performance, the maximum power output of an MFC equipped with PdO–SnO2/rGO as an air cathode is significantly higher than other tested MFCs, reaching 84.6 ± 2.8 mW m−2, which represents ∼ 91 % of the power output of an MFC with Pt/C air cathode. Our results reveal that the incorporation of PdO into transition metal reduced graphene oxide framework has the potential to facilitate their long-term application as air cathodes in MFCs that possess easy fabrication, relatively affordable cost, and outstanding electrochemical catalytic oxygen reduction capability, for enhancing electricity generation from wastewater.

Treatment of aquaculture wastewater by C-MFCs

Chlorella microbial fuel cells (C-MFCs) may be an alternative technology for wastewater treatment. Using microalgae and activated sludge as raw materials, the anode of the MFC was connected in series with a chlorella reactor to establish C-MFCs, so that the simulated aquaculture wastewater was first anaerobically treated in the anode chamber and then aerobically treated in the chlorella reactor. The study found that at a 3:1 N:P ratio and 1000 Ω external resistance, NH3N, COD, and TP removal rates were 69.93 ± 1.84 %, 80.30 ± 3.4 %, and 54.44 ± 4.5 %, respectively, with a maximum chlorophyll content of 0.1049 mg∙m−3. The maximum voltage and maximum power density of 32.94423 mV and 102.37 mW∙m−2. The microbial community structure of the anode chamber was analyzed and the results were as follows: At N:P = 3:1, the dominant bacteria at the genus level in the anode chamber were Klebsiella (47.23 %) and Glucuronobacterium (28.87 %). It can be concluded that N:P affects the power generation capacity of C-MFCs and the removal of NH3N, COD, and TP. The power generation capacity and removal of NH3N, COD, and TP by C-MFCs were best when N:P = 3:1. The regulation of nitrogen and phosphorus in aquaculture wastewater can effectively enhance the performance of C-MFCs.

Polyethylene glycol hydrogel coatings for protection of electroactive bacteria against chemical shocks

Loss of bioelectrochemical activity in low resource environments or from chemical toxin exposure is a significant limitation in microbial electrochemical cells (MxCs), necessitating the development of materials that can stabilize and protect electroactive biofilms. Here, polyethylene glycol (PEG) hydrogels were designed as protective coatings over anodic biofilms, and the effect of the hydrogel coatings on biofilm viability under oligotrophic conditions and ammonia-N (NH4+-N) shocks was investigated. Hydrogel deposition occurred through polymerization of PEG divinyl sulfone and PEG tetrathiol precursor molecules, generating crosslinked PEG coatings with long-term hydrolytic stability between pH values of 3 and 10. Simultaneous monitoring of coated and uncoated electrodes co-located within the same MxC anode chamber confirmed that the hydrogel did not compromise biofilm viability, while the coated anode sustained nearly a 4 × higher current density (0.44 A/m2) compared to the uncoated anode (0.12 A/m2) under oligotrophic conditions. Chemical interactions between NH4+-N and PEG hydrogels revealed that the hydrogels provided a diffusive barrier to NH4+-N transport. This enabled PEG-coated biofilms to generate higher current densities during NH4+-N shocks and faster recovery afterwards. These results indicate that PEG-based coatings can expand the non-ideal chemical environments that electroactive biofilms can reliably operate in.

Sustainable strategies of in-series AEM electrolysis and CEM electrolysis for the treatment of NF concentrates via ettringite-targeted mineral transition

A two-step ion exchange membrane electrolysis process was designed to recover divalent cations (Ca2+, Mg2+) and sulfate from nanofiltration concentrates (NFC) and investigate their removal performance and membrane fouling mechanism. As a result, the AEM electrolysis recovered divalent ions by generating calcium carbonate and magnesium hydroxide in the cathode chamber, while sulfate was enriched in the anode chamber. In the subsequent CEM electrolysis, desalination was achieved by adding chemicals to form ettringite precipitation in the cathode chamber. Meanwhile, the organics in nanofiltration concentrate were removed under the oxidation of platinum-coated titanium electrode and the precipitation adsorption of the cathode chamber. By comparing the desalination performance and organic matter removal, the dosage of ettringite was determined. When the Ca/S = 2.5 and Al/S = 1.0, it was beneficial to the recovery of ettringite. Furthermore, the membrane fouling mechanism was investigated to find that AEM fouling was more severe than CEM. Therefore, this study provides a technical idea and feasibility study for the treatment of municipal nanofiltration concentrate.

The efficient Congo red decolorization coupled with electricity generation via G. sulfurreducens based on microbial fuel cells

In this study, the performance of microbial fuel cells (MFCs) in azo-dye decolorization coupled with electricity generation was evaluated via the model exoelectrogens G. sulfurreducens PCA. The results showed that G. sulfurreducens PCA was capable of decolorizing Congo red (CR) while producing electricity in MFCs. The extracellular reduction of CR by G. sulfurreducens PCA requires the addition of carbon substrate (20 mM NaAc) to generate electrons and then transfer them to the exterior of the cell through respiratory chain proteins. The removal efficiency of 300 mg L−1 CR in the anodic chamber MFCs (98.5%) was found to be 2.30 times higher than that of traditional anaerobic decolorization (42.9%), whereas the maximum output voltage of MFC (0.184 ± 0.011 V) was reduced by 65.2% when compared to that without the addition of CR (0.529 ± 0.014 V). By reducing the initial CR concentration from 300 mg L−1 to 200 mg L−1 and 100 mg L−1, the maximum output voltage of MFCs systems was increased to 0.260 ± 0.042 V and 0.392 ± 0.034 V, respectively, which were 41.3% and 113.0% higher than the 300 mg L−1 CR. Thus, our study stressed that the efficient azo dye decolorization in conjunction with bioelectricity generation could be realized using the MFCs system with model exoelectrogens G. sulfurreducens PCA as the biocatalyst.

Feasibility of CO2 desorption and electrolytic regeneration of potassium carbonate solution in an anion exchange membrane cell

In this work, an electrolytic process was introduced for coupled regeneration of potassium carbonate (K2CO3) solution and water electrolysis by using an anion exchange membrane cell. The process made the CO2 separation from O2 much easier with respect to the existing cationic exchange membrane process. The solution of K2CO3 was used in the cathode chamber to simulate the solution after absorbing CO2. The solution of sulfuric acid (0.1 mol/L H2SO4) was charged in the anode chamber. The feasibility of the process was discussed. The effects of various operation parameters, including temperature, current density, and electrolysis time, were studied. The results indicate that both the yield rate of CO2 and the current efficiency increase initially and decrease afterward with temperature. The yield rate of CO2 increases while the current efficiency decreases with the current density. A low current density can reduce the energy consumption for producing the same amount of CO2. The processes using anion exchange membrane electrolysis can regenerate the absorbent solution to achieve 89% current efficiency, and the simultaneous production of three pure gases, CO2, H2, and O2, makes this method promising.

Electrochemically-mediated treatment of sulfur dioxide and purification of carbon dioxide coupled with hydrogen production

Sulfur dioxide emissions should be and have been regulated due to adverse effects on the environment, and purification of carbon dioxide from low-concentration sources requires energy-intensive steps. Here, we propose incorporating a NaOH-based absorbent with high pH, in which the two gases are simultaneously absorbed, into an electrochemical system that contributes to: (1) mineralizing sulfur dioxide into sulfate; (2) purifying carbon dioxide; and (3) producing hydrogen. Our electrolysis involves primarily anodic sulfite oxidation, and the hydroxide-consuming (proton-generating) sulfite oxidation coupled with cathodic hydrogen evolution allows to generate CO2 with > 85 % purity from an acidifying anodic chamber, and hydrogen evolution from a cathodic chamber with Faradaic efficiency close to 100 %, all at lower energy cost than water electrolysis. This proof-of-concept study demonstrates the feasibility of our functional electrochemical system as a sustainable solution for air pollution control, carbon neutrality, and energy production.

BIOELECTRICITY OF COCOA POD WASTE AS A SUBSTRATE IN A DOUBLE CHAMBER MICROBIAL FUEL CELL

Currently, the utilization of cocoa pod has not been maximized, resulting in waste and foul odors that disturb the environment. However, cocoa pod contain a relatively high amount of cellulose compounds that serve as an energy source or nutrition for bacteria to carry out metabolic activities, thus holding the potential to be used as a substrate in microbial fuel cells (MFC). Based on this, the aim of this research is to investigate the bioelectricity generated by a MFC using cocoa pod husk as a substrate, including voltage, current, and power density, in order to determine the potential of cocoa pod husk waste as an electrical energy source. In this study, a double chamber MFC consists of an anode chamber with copper electrodes and a cathode chamber with zinc electrodes, separated by a salt bridge. The bacteria used were Saccharomyces cerevisiae, and the substrate was made from cocoa pod husk waste. Bioelectricity testing involved measuring the voltage, current, and power density produced by the MFC over several minutes. The measurement results for maximum voltage, maximum current generated, and maximum electrical power (at 6 days) from the reactor were as follows: 36.0 mV, 0.19 mA, and 456 mW/m2, respectively.

Novel bipolar membrane electrolyzer for CO2 reduction to CO in organic electrolyte with Cl2 and NaOH produced as byproducts

Electrochemical reduction of CO2 to valuable products, powered by renewable energy, provides a promising strategy for reducing our dependence on fossil fuels. But up to now, no technology has been implemented for large-scale industrial applications. Without massive utilization of CO2, many vital practical problems, such as reducing CO2 emissions, storing renewable energy, and alleviating environmental pollution, cannot be resolved through this route. Herein, we propose a novel electrolyzer for CO2 electro-reduction, which is separated into three chambers by a bipolar membrane and a cation exchange membrane. In the cathodic chamber, CO2 is reduced to CO in organic electrolytes. In the anodic chamber, Cl- is oxidized to Cl2 in NaCl aqueous solution. In the central chamber, NaOH is obtained. The generated CO and Cl2 can be used as feedstock to produce phosgene (CO+Cl2 =COCl2). Through this route, phosgene can be produced from CO2 and NaCl, with NaOH generated as a byproduct. By substantially increasing the product value, we can promote CO2 electro-reduction technology to industrial applications.

Construction of an ultrasensitive dual-mode chiral molecules sensing platform based on molecularly imprinted polymer modified bipolar electrode

Chiral molecules are abundant in nature. Phenylketonuria (PKU) is caused by the abnormal transformation of chiral molecules L-phenylalanine (L-Phe) in the human blood, which can cause irreversible harm to the human body. In this work, we documented an electrochemiluminescent (ECL) dual-mode sensor platform based on molecularly imprinted polymer (MIP) modified closed bipolar electrodes for high sensitivity detection of L-Phe and D-phenylalanine (D-Phe). In the anode chamber of a bipolar electrode modified with phenylalanine imprinting, Ru (bpy)32+ underwent a redox reaction to produce a chemiluminescence response under the stimulation of a driving voltage. At the same time, the reduction of the cathode film of the bipolar electrode was promoted, and the color changed from dark blue to nearly white. Thus, the dual-mode detection of target molecules is realized. The detection range of the sensor for phenylalanine reached 0.01–10,000 nM, and the detection limits of L-Phe and D-Phe were 3.9 pM and 4.6 pM (S/N = 3), respectively. This dual-mode system achieved high stability and high specificity, and also successfully realized the detection of actual samples, which is expected to achieve future clinical applications.