Animal Caretakers Research Articles

421 An evaluation of Spanish-speaking animal caretakers attitudes towards piglet euthanasia in a large commercial swine production system in Southwestern U.S

421 An evaluation of Spanish-speaking animal caretakers attitudes towards piglet euthanasia in a large commercial swine production system in Southwestern U.S

450 Understanding communication barriers: Demographic variables and language needs in the interaction between English-speaking agriculture professionals and Spanish-speaking animal caretakers

450 Understanding communication barriers: Demographic variables and language needs in the interaction between English-speaking agriculture professionals and Spanish-speaking animal caretakers

DETECTION OF SARS-COV-2 IN A SQUIRREL MONKEY (SAIMIRI SCIUREUS): A ONE HEALTH INVESTIGATION AND RESPONSE.

Through collaborative efforts, One Health partners have responded to outbreaks of COVID-19 among animals, including those in human care at zoos. Zoos have been faced with numerous challenges, including the susceptibility of many mammalian species, and therefore the need to heighten biosecurity measures rapidly. Robust One Health collaborations already exist in Arizona to address endemic and emerging zoonoses, but these have rarely included zoos. The pandemic shed light on this, and Arizona subsequently expanded its SARS-CoV-2 surveillance efforts to include zoo animals. Testing and epidemiologic support was provided to expedite the detection of and response to zoonotic SARS-CoV-2 infection in zoo animals, as well as to understand possible transmission events. Resulting from this program, SARS-CoV-2 was detected from a rectal swab collected from an 8-yr-old squirrel monkey (Saimiri sciureus) from a zoo in Southern Arizona. The animal had rapidly become ill with nonrespiratory symptoms and died in July 2022. Genomic sequencing from the swab revealed mutations consistent with the Omicron (BA.2) lineage. An epidemiologic investigation identified an animal caretaker in close proximity to the affected squirrel monkey who tested positive for COVID-19 the same day the squirrel monkey died. Critical One Health partners provided support to the zoo through engagement of local, state, and federal agencies. Necropsy and pathologic evaluation showed significant necrotizing colitis; the overall clinical and histopathological findings did not implicate SARS-CoV-2 infection alone as a causal or contributing factor in the squirrel monkey's illness and death. This report documents the first identification of SARS-CoV-2 in a squirrel monkey and highlights a successful and timely One Health investigation conducted through multisectoral collaboration.

AABP Guidelines for the Humane Euthanasia of Cattle

For stockman, farmers, veterinarians and animal caretakers who choose to work with cattle there are many rewards, but there is also the responsibility to provide the animals in their care with a good a life, and when that is no longer possible, to alleviating the suffering of a slow and painful death. This is not just between farmers and their cattle it is also part of the social compact farmers have with consumers to produce the food that consumers purchase for their families in a way that is ethical. The requirements for humane euthanasia are also codified in industry animal welfare standards. But in reviewing recent studies on the number of cows and calves that die unassisted or were shipped to slaughter with serious ailments, the reali­ties on farm do not always live up to this expectation. To help to address this the AABP has developed and recently updated the American Association of Bovine Practitioners (AABP) Guidelines for the Humane Euthanasia of Cattle as a tool to help bovine prac­titioners and farmers provide timely and humane euthanasia for the cattle to prevent needless pain and suffering.

PSII-10 Development of an interdisciplinary undergraduate and graduate course in precision livestock farming systems

Abstract The modern livestock industry is increasingly adopting data, technologies, and automation into daily husbandry and management routines and decisions. These advances in precision livestock farming (PLF) enable improved financial margins, sustainability, efficiency, and animal productivity and welfare. However, there is currently a gap between the engineers and data scientists designing the technologies and the animal scientists and caretakers using them. At North Carolina State University a novel course on PLF Systems for undergraduate and graduate students was developed in the Department of Animal Science. Through lecture delivery, livestock production scenarios, scientific literature, and data-based homework assignments students explored data collection, management, analysis, and data-based decision making. Students gained hands-on experience with a variety of sensor types and signal processing through lab-based activities. Basic hardware and software development concepts were explored during an Arduino-based microcontroller activity. The course was developed and refined using student feedback to include additional sensor and lab-based days and additional in-class supported activities utilizing Excel for data manipulation, processing, and presentation. Self-reported student learning results were collected across two semesters (19 students; 18 Animal Science, 1 Biological Engineering) with84% of students indicated the course was effective in increasing their ability to evaluate and implement technology in livestock production systems and 74% of students reported increased knowledge of coding and software, as well as their ability to clean, analyze, and make sense of raw data. The topics and delivery methods implemented in this course could inform the basis for development of similar interdisciplinary PLF courses at other academic institutions.

Understanding Communication Barriers: Demographic Variables and Language Needs in the Interaction between English-Speaking Animal Professionals and Spanish-Speaking Animal Caretakers.

This study focused on assessing the language needs of English-speaking animal professionals in their interactions with Hispanic/Spanish-speaking animal caretakers. A survey was administered to a target audience of non-Spanish speaking and bilingual animal professionals to identify communication gaps while interacting with Hispanic/Spanish-speaking animal caretakers. The data was analyzed with descriptive and inferential statistics, including ordinal regression analyses to examine the impact of demographic variables on respondents' answer choices. The results showed that English-speaking professionals struggled with written and oral communication, which differed compared to bilingual professionals (p < 0.05). Additionally, responses of female professionals varied regarding the aspects of Spanish necessary for interacting with Hispanic/Spanish-speaking animal caretakers, as well as the topics likely to be addressed when agriculture professionals communicate with animal caretakers (p < 0.05). Communication difficulties in the oral medium for both oral receptive skills (listening) and oral productive skills (speaking) were reported as the major barriers that animal professionals need to overcome in their attempt to communicate with the Hispanic/Spanish-speaking workforce in farm settings. This emphasizes the need to address oral communication barriers, and to a lesser degree, the development of reading and writing skills. The topics: typical clinical signs of illness, euthanasia, treatment-drugs, and identification of sick or injured animals were identified as the most likely to be addressed during on-farm interactions. These findings indicate that there are gaps in communication that need to be overcome to improve communication with on-farm Hispanic/Spanish-speaking animal caretakers and consequently contribute to enhancing animal health, welfare, and production.

Standardized Decision-Making for the Selection of Calf and Heifer Rearing Using a Digital Evaluation System

This study addresses the challenge of subjective remounting decisions in calf and heifer rearing, typically driven by the animal caretaker’s feelings and experience, lacking a robust data foundation. Key factors such as developmental delays, diseases, or rearing problems often go unnoticed or are forgotten due to the number of animals. To address this gap, an established state-of-the-art sensor network captures behavioral data during rearing, which is supplemented by manually collected data. This facilitates a novel decision network providing well-founded recommendations to the animal owner regarding whether to retain or cull an animal. The approach focuses on four key areas: colostrum supply, milk intake, weight development, and disease history during the rearing time of each individual, offering a transparent decision path for the use of each future cow. Introducing a standardized decision-making approach, the proposed approach enables an efficient, transparent, and targeted management strategy, contributing to the sustainable enhancement of the health and performance of calves and heifers. Additionally, it allows for the comparison of the growth trajectories of different animals over time. Notably, individual and transparent decisions can be made at each growth stage, enhancing the overall decision-making process in calf and heifer rearing.

The grimace scale: a useful tool for assessing pain in laboratory animals.

Accurately and promptly assessing pain in experimental animals is extremely important to avoid unnecessary suffering of the animals and to enhance the reproducibility of experiments. This is a key concern for veterinarians, animal caretakers, and researchers from the perspectives of veterinary care and animal welfare. Various methods including ethology, immunohistochemistry, electrophysiology, and molecular biology are used for pain assessment. However, the grimace scale, which was developed by taking cues from interpreting pain through facial expressions of non-verbal infants, has become recognized as a very simple and practical method for objectively evaluating pain levels by scoring changes in an animal's expressions. This method, which was first implemented with mice approximately 10 years ago, is now being applied to various experimental animals and is widely used in research settings. This review focuses on the usability of the grimace scale from the "cage-side" perspective, aiming to make it a more user-friendly tool for those involved in animal experiments. Differences in facial expressions in response to pain in various animals, examples of applying the grimace scale, current automated analytical methods, and future prospects are discussed.

Deep Learning Models to Predict Finishing Pig Weight Using Point Clouds.

The selection of animals to be marketed is largely completed by their visual assessment, solely relying on the skill level of the animal caretaker. Real-time monitoring of the weight of farm animals would provide important information for not only marketing, but also for the assessment of health and well-being issues. The objective of this study was to develop and evaluate a method based on 3D Convolutional Neural Network to predict weight from point clouds. Intel Real Sense D435 stereo depth camera placed at 2.7 m height was used to capture the 3D videos of a single finishing pig freely walking in a holding pen ranging in weight between 20-120 kg. The animal weight and 3D videos were collected from 249 Landrace × Large White pigs in farm facilities of the FZEA-USP (Faculty of Animal Science and Food Engineering, University of Sao Paulo) between 5 August and 9 November 2021. Point clouds were manually extracted from the recorded 3D video and applied for modeling. A total of 1186 point clouds were used for model training and validating using PointNet framework in Python with a 9:1 split and 112 randomly selected point clouds were reserved for testing. The volume between the body surface points and a constant plane resembling the ground was calculated and correlated with weight to make a comparison with results from the PointNet method. The coefficient of determination (R2 = 0.94) was achieved with PointNet regression model on test point clouds compared to the coefficient of determination (R2 = 0.76) achieved from the volume of the same animal. The validation RMSE of the model was 6.79 kg with a test RMSE of 6.88 kg. Further, to analyze model performance based on weight range the pigs were divided into three different weight ranges: below 55 kg, between 55 and 90 kg, and above 90 kg. For different weight groups, pigs weighing below 55 kg were best predicted with the model. The results clearly showed that 3D deep learning on point sets has a good potential for accurate weight prediction even with a limited training dataset. Therefore, this study confirms the usability of 3D deep learning on point sets for farm animals' weight prediction, while a larger data set needs to be used to ensure the most accurate predictions.

Zoonoses in endemic, free-ranging mammals

Zoonoses are diseases and infections that can be transmitted naturally between animals and humans. Direct and indirect contact of humans with wildlife occur during hunting activities, when diseased wildlife is found and treated, and in shared fields, forests, parks, gardens, and homes. Zoonoses can only be understood and controlled when ecosystems, animals, and humans are considered holistically. This paper presents important zoonotic pathogens that are currently present in wild mammals as reservoirs in Germany. The literature was searched to determine the prevalence of zoonotic pathogens currently occurring in wild mammals. Viral zoonotic agents currently present in free-ranging, mammalian animals in Germany as reservoirs of natural origin are bornaviruses, lyssaviruses, hepatitisE virus genotype3, and Puumala orthohantavirus. Bacterial zoonotic agents beyond typical wound and foodborne pathogens include Brucella suis Biovar2, Francisella tularensis ssp. holarctica, Leptospira interrogans sensu latu, Mycobacterium caprae, and Yersinia pseudotuberculosis. In particular, parasitic zoonotic agents common in wildlife are Alaria alata, Baylisascaris procyonis, Echinococcus multilocularis, Sacoptes scabei, and Trichinella spp. The presence of zoonotic infectious agents of risk groups2 and3 has to be regularly expected in numerous endemic wildlife species, especially canines, small bears, rodents, insectivores, and bats. Animal caretakers, hunters, veterinarians, and human health professionals should be aware of this risk and take protective measures appropriate to the situation.

Low-dose xenogeneic mesenchymal stem cells target canine osteoarthritis through systemic immunomodulation and homing

BackgroundAs current therapies for canine osteoarthritis (OA) provide mainly symptomatic improvement and fail to address the complex pathology of the disease, mesenchymal stem cells (MSCs) offer a promising biological approach to address both aspects of OA through their immunomodulatory properties.MethodsThis study aimed to investigate the safety and efficacy of xenogeneic MSCs in dogs with OA at different dose levels after intravenous injection. OA was surgically induced in the right stifle joint. Thirty-two male and female dogs were divided into three treatment groups and a control group. Regular general physical examinations; lameness, joint, radiographic, and animal caretaker assessments; pressure plate analyses; and blood analyses were performed over 42 days. At study end, joint tissues were evaluated regarding gross pathology, histopathology, and immunohistochemistry. In a follow-up study, the biodistribution of intravenously injected 99mTc-labeled equine peripheral blood-derived MSCs was evaluated over 24h in three dogs after the cruciate ligament section.ResultsThe dose determination study showed the systemic administration of ePB-MSCs in a canine OA model resulted in an analgesic, anti-inflammatory, and joint tissue protective effect associated with improved clinical signs and improved cartilage structure, as well as a good safety profile. Furthermore, a clear dose effect was found with 0.3 × 106 ePB-MSCs as the most effective dose. In addition, this treatment was demonstrated to home specifically towards the injury zone in a biodistribution study.ConclusionThis model-based study is the first to confirm the efficacy and safety of systemically administered xenogeneic MSCs in dogs with OA. The systemic administration of a low dose of xenogeneic MSCs could offer a widely accessible, safe, and efficacious treatment to address the complex pathology of canine OA and potentially slow down the disease progression by its joint tissue protective effect.

Cultural awareness for veterinarians working with LatinX livestock caretakers

Work done by Colorado State University researchers has high­lighted the human-animal bond that exists on dairy and other livestock operations, and the perceptions of animal caretakers regarding animal health and euthanasia decisions. There is a clear need for culturally responsive training to better prepare animal caretakers to make end-of-life decisions in a timely manner while reducing the emotional burden associated with euthanasia decision making. While veterinarians are well po­sitioned to provide leadership in health management and end-of-life decision-making processes, they are seldom involved in the creation and facilitation of training programs for livestock caretakers. Cultural differences that may limit effective com­munication and training can be overcome by engaging live­stock caretakers with approaches proven effective in both re­search and practice settings.

Shifting attitudes on animal ‘ownership’: Ethical implications for welfare research and practice terminology

The roles companion animals have played in our lives has dramatically changed over the last few decades. At the same time, the terms we use to describe both the people and animals in these human-animal relationships have also changed. One example includes the use of the terms ‘owner’ or ‘guardian’ to refer to the human caretaker. While preferences by society appear to indicate increased interest in referring to companion animal caretakers as ‘guardians’, others have cautioned against its use or attempted to restrict it. Additionally, the use of animal welfare terminology has direct implications for how we conduct both welfare research and practice. Our paper examines the use of ‘owner’ and ‘guardian’ with respect to (1) the implications for changing terminology on the function, clarity and uniformity of their use, and (2) the ethical and welfare impact that coincides with each term’s use. Our goal is to propose terminological considerations that could influence future welfare research, as well as help guide our interactions with companion animals.

Harmonisation of education, training and continuing professional development for laboratory animal caretakers, technicians and technologists: Report of the FELASA-EFAT Working Group.

Competent, confident and caring laboratory animal caretakers, technicians and technologists (LAS staff) are vital for good animal welfare, high-quality science and a secure Culture of Care. This requires high-quality education, training, supervision and continuing professional development (CPD) of LAS staff. However, there is a lack of harmonisation regarding how this education and training is conducted among European countries, and nor are there recommendations adapted to Directive 2010/63/EU. Therefore, FELASA and EFAT established a working group with the task of establishing recommendations for education, training and CPD for LAS staff. The working group established five different levels (LAS staff levels 0-4), defining the required level of competence and attitude, as well as suggesting educational requirements for reaching each level. Defining these levels should help to ensure that appropriate educational and CPD activities are in place, and to enable employers and LAS staff to determine the level and career stage attained. Furthermore, proper assessment of competencies and effective CPD schemes for all relevant staff should be established. Regulators should support this by setting standards for competence assessment and ensuring that they are consistently applied. In addition, establishments should involve the LAS staff in defining and developing the Culture of Care. The Animal Welfare Body should be involved and have oversight of education, training and CPD. These recommendations will contribute to harmonisation and increased quality of education, training and CPD, as well as provide clearer career pathways for LAS staff, helping to ensure high standards of animal welfare and science.

Learning outcomes for the education and training of laboratory animal caretakers in South Africa.

Education and training is essential for laboratory animal caretakers (LACs), but there are no courses available in South Africa. A national workshop was thus held to collaboratively establish the learning outcomes (LOs) for the education and training (E&T) of LACs. Eighty-five stakeholders from 30 institutions took part in small group discussions interspersed with plenary sessions to draw up the consensus LOs. One-hundred-and-twenty LOs were identified, grouped into the following three main themes and 15 topics: 1) Focus on animals (animal care and husbandry, animal ethics, animal welfare, basic biology, environment); 2) Focus on humans (administration, health and safety, lifelong learning, professionalism, psychological wellbeing); and 3) Focus on systems (biosecurity, equipment, jurisprudence, logistics, and quality management). This E&T framework provides a foundation for a career path in the laboratory animal science field. The psychological (i.e. mental and emotional) wellbeing of LACs forms a noteworthy component of the focus on humans, since working with research animals is stressful and coping mechanisms are needed in order to promote compassion satisfaction and prevent compassion fatigue and burnout. Approximately 75% of the LOs are knowledge-based, while 25% are competencies in practical skills. It is recommended that competencies should be assessed by direct observation of practical/procedural skills, where competence in a procedure or practical task is assessed against predetermined criteria. These LOs are published with the intent that they will promote animal and human wellbeing, support ethical science, maintain public confidence, and in so doing, contribute to a just and civilised society.

Doing better for understudied species: Evaluation and improvement of a species-general animal welfare assessment tool for zoos

Accredited zoos and aquariums are required to have a welfare assessment process in place for the animals in their care. However, welfare assessment theoretical frameworks and methodologies vary widely. Previous studies have shown that taxonomic biases in research are found broadly within the field of animal behavior and specifically within applied animal welfare science, which could translate to taxonomic biases in applied welfare assessment tools. After creating and then using a species-general welfare assessment at Lincoln Park Zoo for 2.5 years, we sought to examine whether there were systematic differences in welfare scores for different taxa. There were 939 Welfare Discussion Tools completed for 174 species between July 2019 and – December 2021, including mammals, birds, reptiles, amphibians, fish, and invertebrates. We found that, broadly, no taxonomic group scored higher than mammals on overall welfare scores, input (resource-based) scores, or output (animal-based) scores. Raters also provided fewer incomplete responses (in the form of “not applicable” or “I don’t know” judgments) for mammals than for other taxonomic groups. Integrating these findings with input from welfare scientists, animal caretakers, behavioral husbandry experts and veterinary staff, we modified our original welfare assessment tool to broaden the taxonomic relevance of the tool. Copies of the original tool and the updated/modified tool are included to serve as a model for institutions developing or revising their own welfare assessment tools.

Recognizing Zooeyia to Promote Companion Animal Welfare in Urban Bangladesh.

The One Health concept of zooeyia refers to the benefits of companion animals in human health and is gaining global research attention. This exploratory study aimed to understand contemporary experiences and perceptions of the social benefits and challenges of living with a companion animal in urban Bangladesh. Thirty-five qualitative interviews were conducted with companion animal owners (20), animal sellers (10), and livestock service department officers (5) from two major cities in Bangladesh, Dhaka and Khulna. Thematic analysis found that historically, animals had a utilitarian purpose, such as livestock for food and dogs for security. The role and perceptions of companion animals began to change for some around the turn of the century. Today, companion animal caretakers report social, psychological and physical health benefits from integrating companion animals into their lives. They also report that companion animal ownership can contribute to social problems due to the prevailing stigma against companion animals. This is rooted in the continued utilitarian role attached to companion animals by the majority of the Bangladesh population as well as religious-based non-acceptance. As a result, the Animal Welfare Act (2019) is not well implemented, posing a key concern for companion animal welfare. To tackle this, we propose various ways in which the emerging concept of zooeyia can help promote the welfare of companion animals by challenging the stigma associated with them in Bangladesh.

Automated monitoring and detection of disease using a generic facial feature scoring system - A case study on FMD infected cows.

Digital images are becoming more readily available and possibilities for image processing are developing rapidly. This opens the possibility to use digital images to monitor and detect diseases in animals. In this paper we present 1) a generic facial feature scoring system based on seven facial features, 2) manual scores of images of Holstein Frisian heifers during foot-and-mouth disease vaccine efficacy trials and 3) automatic disease scores of the same animals. The automatic scoring system was based on the manual version and trained on annotated images from the manual scoring system. For both systems we found an increase in disease scores three days post infection, followed by a recovery. This temporal pattern matched with observations made by animal caretakers. Importantly, the automatic system was able to discern animals that were protected by the vaccine, and did not develop blisters at the feet, and animals that were not protected. Finally, automatic scores could be used to detect healthy and sick animals with a sensitivity and specificity of 0.94 on the second and third days following infection in an experimental setting. This generic facial feature disease scoring system could be further developed and extended to lactating Holstein Frisian dairy cows, other breeds and other infectious diseases. The system could be applied during animal experiments or, after further development, in a farm setting.

Pheromones for amphibian conservation – considering new approaches in conservation breeding programmes

Pheromones for amphibian conservation – considering new approaches in conservation breeding programmes

Precalving behavior in dairy cattle with different calving times.

Predicting the calving time in dairy cattle can help in avoiding calving accidents and reducing burdens on animal caretakers. In this study, we analyzed the behavior of pregnant dairy cattle for 7 days prior to calving, to assess the feasibility of predicting the calving time. Eleven Holstein cows were divided into two groups based on their calving times, that is, in the morning (the Morning Parturition Group) or the evening (the Evening Parturition Group). Their behavior was recorded on video. An analysis was conducted of the daily occurrences of each type of behavior and the number of switches of behavior during the day and at night. A statistical analysis was conducted, using a two-way factorial analysis. The behavioral sequence was analyzed using an adjacency matrix. Hierarchical structure charts were created, using Interpretive Structural Modeling. The results suggest that feeding and exploratory behaviors are associated with the calving time period and thus can be useful when predicting that period. The hierarchical structure charts suggest that the Morning Parturition Group had no definite behavioral sequence pattern, unlike the Evening Parturition Group. The detection of an unstable behavioral sequence pattern might predict the calving time period.