Anatomical Methods Research Articles

Dendrochemical forensics as material evidence in courts: How could trees lie?

The legal admissibility of scientific tools, such as dendrochemistry providing forensic evidence for criminal or civil cases, critically relies on the quality of fundamental and applied scientific research. The “Daubert” and “Frye” criteria that federal courts in the U.S.A use for determining legal admissibility requires publication of the scientific basis for the tool, and general acceptance by the scientific community. The field of dendroforensics is rapidly evolving, with new methods constantly being developed. In this manuscript we investigate how this dendrochemical evidence has been used successfully in the courtroom. The study of tree rings using physical anatomical and dendrochronological methods has been used as evidence in courts for over 150 years. From these beginnings in dendroecology dendrochemical and biological methods have matured enough to allow it to be used in forensic investigations, finding applications as a new independent line of evidence around the world, supporting cases involving murder, trafficking of protected species, and pollution crimes. We summarize some of the key applications of dendrochemistry in forensic cases and illustrate them with courtroom examples. The basic analytical methods discussed (e.g., PCR, GC-MS, LIBS, LA/ICP-MS, EDXRF) are all conventional. However, for findings to be relevant to judicial cases, the data is normally applied with additional lines of evidence gathered such as tree physiology and relevant statistics. This can allow us to gain more powerful data to help age date a specific event or to spatially identify a source material. The purpose of this article is to show how recent research has paved the way for the use of dendrochemical evidence in courts. It shows how dendrochemistry can be useful for forensic investigations including: murder cases, trafficking of protected species, and pollution crimes. The applications are illustrated by several summarized legal cases, but due to the confidential nature of some of these cases it was not always possible to provide full details or references.

Pterocarpus wood identification by independent and complementary analysis of DART-TOFMS, microscopic anatomy, and fluorescence spectrometry

Abstract Attention to illegal logging practices and demanding policies in transnational timber trade have driven the need for species-level identification of timber. Historically wood has been identified to genus level using microscopy and anatomical characteristics, however, new chemometric and imaging methods have been developed to increase the speed and precision of timber identification. This study approaches species identification using a combination of complementary methods: Direct Analysis in Real Time–Time-of-Flight Mass Spectrometry (DART-TOFMS), wood anatomy, and fluorescence spectroscopy. Seven commercially and environmentally significant species in Pterocarpus, including P. erinaceus (CITES Appendix II), P. santalinus (CITES Appendix II), P. tinctorius (CITES Appendix II), P. indicus, P. macrocarpus, P. dalbergioides, and P. soyauxii were studied. It was found that DART-TOFMS paired with discriminant analysis of principal components (PCA) could classify species with an accuracy of 95–100%, while anatomy in combination with PCA applied to fluorescence spectra could be used to classify CITES Appendix II species. In the absence of access to DART-TOFMS, a combination of wood anatomy and fluorescence spectrometry can permit more accurate identification than anatomy alone.

Three-Dimensional Quantification of Glenoid Bone Loss in Anterior Shoulder Instability: The Anatomic Concave Surface Area Method.

Background:Recurrent shoulder instability may be associated with glenoid erosion and bone loss. Accurate quantification of bone loss significantly influences the contemplation of surgical procedure. In addition, assessment of bone loss is crucial for surgical planning and accurate graft placement during surgery.Purpose:To quantify the concave surface area of glenoid bone loss by using 3-dimensional (3D) segmented models of the scapula and to compare this method with the best-fit circle and glenoid height/width methods, which use the glenoid rim for bone loss estimations.Study Design:Cohort study (diagnosis); Level of evidence, 2.Methods:A total of 36 consecutive preoperative bilateral computed tomography scans of patients eligible for a primary Latarjet procedure were selected from our institutional surgical database (mean patient age, 29 ± 9 years; 31 men and 5 women). The 3D models of both scapulae were generated using medical segmentation software and were used to map the anatomic concave surface area (ACSA) of the inferior glenoid using the diameter of the best-fit circle of the healthy glenoid. Bone loss was calculated as a ratio of the difference between surface areas of both glenoids (healthy and pathological) against the anatomic circular surface area of the healthy glenoid (the ACSA method). These results were compared with bone loss calculations using the best-fit circle and glenoid height/width methods. Inter- and intraobserver reliability were also calculated.Results:The mean (± SD) bone loss calculated using the ACSA, the best-fit circle, and glenoid height/width methods was 9.4% ± 6.7%, 14.3% ± 6.8%, and 17.6% ± 7.3%, respectively. The ACSA method showed excellent interobserver reliability, with an intraclass correlation coefficient (ICC) of 0.95 versus those for the best-fit circle (ICC, 0.71) and glenoid height/width (ICC, 0.79) methods.Conclusion:Quantification of instability-related glenoid bone loss is reliable using the 3D ACSA method.

Logic, geometry and visualisation of the body in Acquapendente’s rediscovered Methodus anatomica (1579)

AbstractThe article provides the first description and analysis of the recently rediscovered manuscript titled Methodus anatomica by Girolamo Fabrici da Acquapendente (1533–1619). Acquapendente was one of the most important anatomists in late sixteenth-century Europe and played an instrumental role as Harvey’s teacher in Padua towards the latter’s discovery of the circulation of the blood. The manuscript provides first-hand testimony as to how anatomy was administered in Padua in the post-Vesalian era and sheds light on a number of otherwise unknown aspects of the development of the anatomical method. Chiefly among these is the attention devoted by Acquapendente to historia, as a way to order sensory data in a consistent way, which draws widely from the geometrical method and from the contemporary debate on the discretisation of continuous quantities.

Lumen diameter variation of hand radial artery in cadaver anatomical snuffbox

Background: The most recent finding in interventional cardiology is more distal radial accesses. The success rate of this access reached 97,7%. Variation in lumen diameter at the puncture site can be a contributing factor to access failure. However, this variation has not been well recorded due to the absence of studies regarding it. Aim: To determine the variation of the lumen diameter of the distal radial artery in the anatomical snuffbox. Methods: This study was an observational study on 13 cadavers with a total of 25 hands. Dissection of the cadaver was done by the anatomical dissection method, and the lumen diameter was measured. Results: Descriptive measurement showed significant variation in lumen diameter of the distal radial artery. Conclusion: There is medium to very high variation in lumen diameter based on hand side, gender, and both.

Ultrasound-guided penile block for circumcision instead of anatomical landmark method in newborn babies

BackgroundThe primary objective of this study is to compare the effectiveness of the newborn’s penile block performed by the surgeon using the classical landmark method and the penile block performed by the anesthesiologist with ultrasound guidance.ResultsThis prospective, single-blinded, randomized clinical study included a total of forty newborn babies scheduled to undergo elective circumcision. The babies were randomized into two treatment groups of ultrasound (US)-guided penile block (group I; n = 20) and classical landmark method dorsal penile nerve block (DPNB) (group II; n = 20) group. Face, Legs, Activity, Cry, Consolability (FLACC) score was used to determine the block efficacy and postoperative pain and analgesic requirements. Intraoperative and postoperative FLACC scores, intraoperative analgesic needs, discharge time, complications, first oral intake time, and parent’s satisfaction were also recorded.FLACC scores were higher in group II than in group I during the intraoperative periods. Heart rate was higher in group II than in group I, at the incision, and during the procedure (P < 0.05). The number of patients requiring fentanyl was higher in group II than in group I (P < 0.01). FLACC scores were statistically higher in the landmark group at arrival in the PACU (P < 0.01) and after 30 min up to 2 h (P < 0.01). Parent’s satisfaction was significantly higher in US group (P < 0.01)ConclusionsIntraoperative analgesic needs and pain scores are lower in newborn babies who performed penile block with ultrasound-guided compared to the landmark method.

Topographical, anatomical and neurosurgical aspects of "end-to-side" nerve repair

The article presents the results of a comprehensive anatomical and experimental study of individual variability in the structure and topography of motor branches of peripheral nerves in relation to the justification of methods for selective reinnervation of tissues by the "end-to-side" neurorrhaphy. It was found that relatively longer branches of peripheral nerves with a small number of connecting inter-arm collaterals characteristic of narrow and long limbs create conditions for less traumatic mobilization of motor branches. In cases with relatively wide and short extremities mobilization of peripheral nerves is complicated by the presence of a large number of collateral branches and intra-trunk connections, which are often damaged when separate bundles that make up the mobilized branches of the donor or recipient nerve are isolated from the main nerve trunk. It has been shown that potential recipient nerves should be motor branches of peripheral nerves, the preservation of which is of fundamental importance for the function of the corresponding segment of the limb. To create conditions conducive to selective reinnervation of functionally significant muscle groups of the upper limb, we have developed, justified from anatomical positions, and tested in an experiment on anatomical material methods for connecting the distal motor branches of peripheral nerves by the "end-to-side" neurorrhaphy. The main idea of accelerated recovery of the thumb opposition in injuries of the median nerve is to reinnervate the muscles of the elevation of the I finger due to nerve fibers that are part of the deep branch of the ulnar nerve. For this purpose, surgical techniques have been developed for connecting the recurrent motor branch of the damaged median nerve mobilized at the level of the wrist with the edges of a surgically formed perineurium defect on the lateral surface of the bundles that make up the deep branch of the ulnar nerve. In another clinical situation, in patients with radial nerve injuries, for the muscle reinnervation, а method is proposed for neurotisation of the deep motor branch of the radial nerve by the end-to-side suture to the lateral surface of the median nerve. We assume that performing the "end-to-side" nerve suture at the level of the base of the hand in the cases of proximal damage to the median nerve will reduce the time of reinnervation of the muscles of the thumb elevation by 400450 days. Transposition of the deep branch of the damaged at the proximal level radial nerve with "end-to-side" neurorrhaphy to the median nerve by 250300 days (based on the total length of the shoulder and forearm, which is about 50 cm and the rate of regeneration of nerve fibers 1 mm per day). Accordingly, with higher injuries (brachial plexus), the gain in the time of reinnervation of the distal segments will be even greater. In our opinion, the results can be used as a basis for further clinical research on the development of methods for selective tissue reinnervation in cases with isolated injuries of the peripheral nerves.

The achievements and prospects of the research conducted by the Embryology and Biotechnology Laboratory

The article presents the achievements of the Embryology and Biotechnology Laboratory obtained in the last ten years. The laboratory was founded in 1964 based on the group of plant cytology, becoming an independent subdivision within the Botanical Garden led in that period by the academician Alexandru Ciubotaru. Since 1995, the biotechnology group has been working in the laboratory under the leadership of the undersigned. Biotechnological methods arose from embryological, cytological, anatomical and morphophysiological methods, taking on a large scale in the second half of the 20th century. In the last decade, about 40 micropropagation technologies for plants with increased economic value have been developed in the laboratory.

Physiological Roles of the Rapidly Activated Delayed Rectifier K+ Current in Adult Mouse Heart Primary Pacemaker Activity.

Robust, spontaneous pacemaker activity originating in the sinoatrial node (SAN) of the heart is essential for cardiovascular function. Anatomical, electrophysiological, and molecular methods as well as mathematical modeling approaches have quite thoroughly characterized the transmembrane fluxes of Na+, K+ and Ca2+ that produce SAN action potentials (AP) and ‘pacemaker depolarizations’ in a number of different in vitro adult mammalian heart preparations. Possible ionic mechanisms that are responsible for SAN primary pacemaker activity are described in terms of: (i) a Ca2+-regulated mechanism based on a requirement for phasic release of Ca2+ from intracellular stores and activation of an inward current-mediated by Na+/Ca2+ exchange; (ii) time- and voltage-dependent activation of Na+ or Ca2+ currents, as well as a cyclic nucleotide-activated current, If; and/or (iii) a combination of (i) and (ii). Electrophysiological studies of single spontaneously active SAN myocytes in both adult mouse and rabbit hearts consistently reveal significant expression of a rapidly activating time- and voltage-dependent K+ current, often denoted IKr, that is selectively expressed in the leading or primary pacemaker region of the adult mouse SAN. The main goal of the present study was to examine by combined experimental and simulation approaches the functional or physiological roles of this K+ current in the pacemaker activity. Our patch clamp data of mouse SAN myocytes on the effects of a pharmacological blocker, E4031, revealed that a rapidly activating K+ current is essential for action potential (AP) repolarization, and its deactivation during the pacemaker potential contributes a small but significant component to the pacemaker depolarization. Mathematical simulations using a murine SAN AP model confirm that well known biophysical properties of a delayed rectifier K+ current can contribute to its role in generating spontaneous myogenic activity.

A Novel Technique to Identify the Latissimus Dorsi Muscle During Modified Radical Mastectomy

Breast cancer is the most common cancer affecting women in India and worldwide. Surgery is the primary treatment of nonmetastatic breast cancer. Modified radical mastectomy (MRM) is a very important aspect of the surgical training in general surgery and surgical oncology. The lateral limit of the dissection is the latissimus dorsi (LD) muscle. In the early part of the learning curve, many trainees tend to overshoot the border of the LD muscle and enter into the posterior plane. The technique described is a simple anatomical method of identifying the anterior border of the LD during MRM.

Application of ultrasound-guided selective nerve branch blockage in lumbar spinal nerve posterior branch syndrome

To investigate the efficacy and safety of ultrasound-guided selective nerve branch blockage in the treatment of lumbar spinal nerve posterior branch syndrome. A total of 40 patients with lumbar spinal nerve posterior branch syndrome treated by Pain Clinic from May 2017 to December 2018 were selected. According to the method used in locating site for nerve blockage, the patients were divided into ultrasound-guided group and anatomical positioning group, with 20 cases in each group. In anatomical positioning group, there were 7 males and 13 females, aged (63.42±7.71) years old, weighted (63.65±10.72) kg, numerical rating scale (NRS) was (6.61±1.52) scores, course of disease was (16.55±4.68) months. Pain sites:4 cases at L2,3, 8 cases at L3,4, 11 cases at L4,5, and 11 cases at L5S1. In ultrasound-guided group, there were 10 males and 10 females, aged (59.58±10.21) years old, weighted (60.61±13.81) kg, NRS was(6.84±2.43) scores, and course of disease was(13.70±5.98) months. Pain sites:6 cases at L2,3, 6 cases at L3,4, 9 cases at L4,5, and 13 cases at L5S1. Ultrasound-guided group used ultrasound-guided selective posteromedial branch and posterolateral branch nerve blockage, and the anatomical positioning group used anatomical localization method to block the posteromedial branch and posterolateral branch of lumbar spinal nerve. Each nerve branch was injected 3 ml of 0.125% ropivacaine. The number of treatment required and prone position time of each treatment were recorded, and the NRS scores of patients at the time points of immediately after the end of the treatment, the first week, the second week, the first month and the third month were evaluated. And adverse events such as local anesthetic allergy and poisoning, local puncture infection, total spinal anesthesia, dizziness, drowsiness, nausea, vomiting and other adverse reactions were observed. There were no statistically significant differences in gender, age, weight, NRS, course of disease and pain segment distribution between two groups (P>0.05). The number of treatment required in anatomical positioning group was significantly higher than that in ultrasound-guided group (P<0.000 1). During each treatment, the time in the prone position of the patients in anatomical positioning group was significantly lower than that in ultrasound guided group (P< 0.000 1). NRS scores immediately after the end of treatment, 1 week, 2 weeks, 1 month and 3 months, anatomical positioning group were 2.98 ±0.25, 3.04 ±0.38, 3.37 ±0.47, 3.42 ±0.85, 3.50 ±0.43, respectively, 2.94 ±0.31, 3.00 ±0.29, 3.21 ±0.68, 3.16 ± 0.94, 3.17±0.53 in ultrasound-guided group, and there was significant difference at 1 month and 3 months between two groups(P< 0.05). There were no adverse events such as local anesthetic allergy and poisoning, local puncture infection, and total spinal anesthesia, and no adverse reactions such as lethargy, nausea, and vomiting occurred in two groups. There were 6 cases of dizziness in anatomical positioning group and 12 cases in ultrasound guided group. The difference between two groups was statistically significant(P<0.05). Comparedwith anatomicalpositioning, ultrasound-guided selective nerve branch block for the treatment of posterior branch of the lumbar spinal cord syndrome can reduce the number of treatments and maintain a longer therapeutic effect, but it is also necessary to pay attention to the time of each treatment to avoid dizziness and other adverse reactions.

PHYSICAL ACTIVITY LEVEL RESTORATION IN PATIENTS AFTER ANTERIOR CRUCIATE LIGAMENT RECONSTRUCTION USING «BONE-TENDON-BONE» AUTOGRAFT

Injuries to the anterior cruciate ligament of the knee joint, while remaining one of its most common types of injury, cause restriction of physical activity in people leading an active lifestyle, due to the occurrence of biomechanical disorders. The generally accepted approach of surgical correction is aimed at replacing the lost structure in order to approach the motor norm of the preserved elements of the joint. The presented publication reflects the results of arthroscopic surgical reconstruction of the anterior cruciate ligament with an autograft from the patellar ligament by anatomical and transtibial methods. The main methods of diagnosing tears of the anterior cruciate ligament were clinical and instrumental studies, including manual testing, radiography, magnetic resonance imaging, hardware arthrometry. A total of 134 patients with long-standing damage to the anterior cruciate ligament and chronic anterior instability of the knee joint were treated, which were divided into two groups according to the method of plastic surgery. Complete recovery of the level of physical activity and return to sports were observed in the majority of patients of both clinical groups.

A REVIEW ON ANATOMICAL METHODS IN PLANT SYSTEMATICS

The comparative study of plant structure, morphology, and anatomy has always been the backbone of plant systematics. Anatomical characteristics are important evidence when elucidating the relationships among higher plants. Therefore the anatomical information can be taxonomically useful without having obvious evolutionary or phylogenetic interpretation. This review is a comprehensive study of the plant anatomical methods used by various researchers using different plant sources like leaf, petiole, and stem

Improved Accuracy of Amyloid PET Quantification with Adaptive Template-Based Anatomic Standardization.

Amyloid PET noninvasively visualizes amyloid-β accumulation in the brain. Visual binary reading is the standard method for interpreting amyloid PET, whereas objective quantitative evaluation is required in research and clinical trials. Anatomic standardization is important for quantitative analysis, and various standard templates are used for this purpose. To address the large differences in radioactivity distribution between amyloid-positive and amyloid-negative participants, an adaptive-template method has been proposed for the anatomic standardization of amyloid PET. In this study, we investigated the difference between the adaptive-template method and the single-template methods (use of a positive or a negative template) in amyloid PET quantitative evaluation, focusing on the accuracy in diagnosing Alzheimer's disease (AD). Methods: In total, 166 participants (58 healthy controls [HCs], 62 patients with mild cognitive impairment [MCI], and 46 patients with AD) who underwent 11C-Pittsburgh compound B (11C-PiB) PET through the Japanese Alzheimer's Disease Neuroimaging Initiative study were examined. For the anatomic standardization of11C-PiB PET images, we applied 3 methods: a positive-template-based method, a negative-template-based method, and an adaptive-template-based method. The positive template was created by averaging the PET images for 4 patients with AD and 7 patients with MCI. Conversely, the negative template was created by averaging the PET images for 8 HCs. In the adaptive-template-based method, either of the templates was used on the basis of the similarity (normalized cross-correlation [NCC]) between the individual standardized image and the corresponding template. An empiric PiB-prone region of interest was used to evaluate specific regions where amyloid-β accumulates. The reference region was the cerebellar cortex, and the evaluated regions were the posterior cingulate gyrus and precuneus and the frontal, lateral temporal, lateral parietal, and occipital lobes. The mean cortical SUV ratio (mcSUVR) was calculated for quantitative evaluation. Results: The NCCs of single-template-based methods (the positive template or negative template) showed a significant difference among the HC, MCI, and AD groups (P < 0.05), whereas the NCC of the adaptive-template-based method did not (P > 0.05). The mcSUVR exhibited significant differences among the HC, MCI, and AD groups with all methods (P < 0.05). The mcSUVR area under the curve by receiver operating characteristic analysis between the positive group (MCI and AD) and the HC group did not significantly differ among templates. With regard to diagnostic accuracy based on mcSUVR, the sensitivity of the negative-template-based and adaptive-template-based methods was superior to that of the positive-template-based method (P < 0.05); however, there was no significant difference in specificity between them. Conclusion: In quantitative evaluation of AD by amyloid PET, the adaptive-template-based anatomic standardization method had greater diagnostic accuracy than the single-template-based methods.

The Symbiosis of Leigh Hunt and Benjamin Haydon: Focusing on an Argument about Blacks

This essay examines the symbiotic relationship between Leigh Hunt and Benjamin Haydon within the context of the aesthetic cultural phenomenon in the early nineteenth century. By paying special attention to the Examiner used as their debate table, this essay investigates how these Cockneys’ independent voices about blacks were against, but also nurtured and advanced, each other. First, this essay explores how Hunt and Haydon constructed a friendship based on their passion for the arts. Afterward, it examines how Hunt argued against Haydon’s anatomical sketch method based on the racial hierarchy in accordance with Hunt’s humanitarian interests in blacks. Subsequently, the essay goes on to look at how Haydon defended himself against Hunt’s attack by asserting his artistic point of view about blacks. Although re-examining the very private story of friendship between Hunt and Haydon can be viewed as narrow, this essay certainly shows us a picture of the most sociable and aesthetic cultural view of the period through their friendship.

Multi-modal imaging of high-risk ductal carcinoma in situ of the breast using C2Am: a targeted cell death imaging agent

BackgroundDuctal carcinoma in situ (DCIS) is a non-invasive form of early breast cancer, with a poorly understood natural history of invasive transformation. Necrosis is a well-recognized adverse prognostic feature of DCIS, and non-invasive detection of its presence and spatial extent could provide information not obtainable by biopsy. We describe here imaging of the distribution and extent of comedo-type necrosis in a model of human DCIS using C2Am, an imaging agent that binds to the phosphatidylserine exposed by necrotic cells.MethodsWe used an established xenograft model of human DCIS that mimics the histopathological features of the disease. Planar near-infrared and optoacoustic imaging, using fluorescently labeled C2Am, were used to image non-invasively the presence and extent of lesion necrosis.ResultsC2Am showed specific and sensitive binding to necrotic areas in DCIS tissue, detectable both in vivo and ex vivo. The imaging signal generated in vivo using near-infrared (NIR) fluorescence imaging was up to 6-fold higher in DCIS lesions than in surrounding fat pad or skin tissue. There was a correlation between the C2Am NIR fluorescence (Pearson R = 0.783, P = 0.0125) and optoacoustic signals (R > 0.875, P < 0.022) in the DCIS lesions in vivo and the corresponding levels of cell death detected histologically.ConclusionsC2Am is a targeted multi-modal imaging agent that could complement current anatomical imaging methods for detecting DCIS. Imaging the presence and spatial extent of necrosis may give better prognostic information than that obtained by biopsy alone.

Collegiate athlete brain data for white matter mapping and network neuroscience

We describe a dataset of processed data with associated reproducible preprocessing pipeline collected from two collegiate athlete groups and one non-athlete group. The dataset shares minimally processed diffusion-weighted magnetic resonance imaging (dMRI) data, three models of the diffusion signal in the voxel, full-brain tractograms, segmentation of the major white matter tracts as well as structural connectivity matrices. There is currently a paucity of similar datasets openly shared. Furthermore, major challenges are associated with collecting this type of data. The data and derivatives shared here can be used as a reference to study the effects of long-term exposure to collegiate athletics, such as the effects of repetitive head impacts. We use advanced anatomical and dMRI data processing methods publicly available as reproducible web services at brainlife.io.

MORPHOFUNCINAL CHARACTERISTIC OF THYMUS IN SEVEN MONTHLY RATS UNDER CONDITIONS OF MOUNTAIN HYPOXIA OF KYRGYZSTAN

In recent years, the problem of hypoxia has attracted increasing attention of experimenters and clinicians, since studying various aspects of hypoxia has shown the universal role of short-term or longer-term oxygen deficiency in the regulation of body activity and the development of pathology. The most populated are the regions located in the low mountains (from 200 to 1400 m above sea level) and the middle mountains (from 1400 to 2500 m). The highlands fall on a height of up to 3200 m. Above is a non-residential snow highlands and above the highlands. A study of the histology of thymus was carried out in 62 seven-month-old rats living in various environmental and climatic conditions in Kyrgyzstan. Morphofunctional structures of the thymus gland in seven monthly rats were studied. Study methods: 1. Anatomical methods (preparation). 2. Histological methods (hemotoxylin-eosin coloring, according to Van Gizon). As a result of a study of seven monthly rats in Bishkek (770 m above sea level) under low-altitude conditions, all control group values are within normal limits. Indicators in Tuya-Ashu in the highlands (3200 m above sea level), a noticeable change in cells, for example, the number of lymphoblast indicators has increased. In the conditions of the middle mountain Cholpon-Ata (1660 m above sea level), cell performance decreased. The results of this study of seven monthly rats in the conditions of the highlands of Naryn (2000 m above sea level), the dynamics of cell populations in the unit of the conditional area of the cortical substance of the timus slice in tseven monthly rats showed a slight decrease in cell performance compared to the average Cholpon-Ata. Thus, Bishkek in low-altitude conditions, i.e. 770 m above sea level, all indicators of the control group are within the norm. In the highlands (3200 m above sea level), a noticeable change in cells, for example, the number of lymphoblasts increased by 3.44%, average lymphacites increased by 2.03%, small lymphacites by 316.8%. Apoptotic bodies 59.8%, mitoses by 2.1%, Taurus Gassal increased by 0.09%. Macrophage index increased by 0.12%. Stereometric characteristic of crustal thymus substance of seven monthly rats 26 .67%. The cerebral matter increased by 6.99%., The intra-pulmonary perivascular space (runway) by 1.66%. Interdigital septs increased by 7.98%. Keywords: Timus. seven-month-old rats. Lowland, Midland and Highlands

Cardioprotective Effect of Crude Extract and Solvent Fractions of Urtica simensis Leaves on Cyclophosphamide-Induced Myocardial Injury in Rats.

BackgroundGlobally, cardiovascular diseases (CVDs) are becoming the major cause of death. Urtica simensis is one of endogenous plant which treats a wide range of disease conditions including heart diseases. However, there is limited information on safety and efficacy of the plant.ObjectiveTo evaluate the in vitro antioxidant, the in vivo cardioprotective activity of crude extract and solvent fractions of Urtica simensis leaves on cyclophosphamide-induced myocardial injury.MethodsThe cardioprotective activity of the crude extract, aqueous and hexane fraction of Urtica simensis leaves was evaluated based on anatomical, biochemical and histopathological methods. The in vitro antioxidant activity of the plant was also assayed in terms of free radical scavenging activity (RSA).ResultsCrude extract and solvent fractions of Urtica simensis significantly prevented the deleterious effect of cyclophosphamide on body weight (P<0.001), heart weight to body ratio (P<0.01), cardiac biomarkers including troponin I (P<0.01), alanine transaminase (ALT) (P<0.001), aspartate aminotransferase (AST) (P<0.01) and lipid profiles including triglycerides (P<0.001) and total cholesterol (P<0.01). The histopathological study confirmed presence of necrosis, oedema and haemorrhage on cyclophosphamide alone-treated groups while the 200mg/kg and 400mg/kg of the crude extract and aqueous fraction showed normal cardiocytes. The antioxidant assay of Urtica simensis plant exhibited free radical scavenging activity of inhibitory concentration of 50% (IC50) for the crude extract with the values of 63.27µg/mL, aqueous fraction with the values of 136.38µg/mL and hexane fraction with the values of 258.70µg/mL.ConclusionCrude extract and solvent fractions of Urtica simensis leaves have cardioprotective activities. The cardioprotective effect could be attributed to the antioxidant activity of the plant extracts. However, this requires further in-depth understanding.

The cortical origin and initial spread of medial temporal tauopathy in Alzheimer's disease assessed with positron emission tomography.

Advances in molecular positron emission tomography (PET) have enabled anatomic tracking of brain pathology in longitudinal studies of normal aging and dementia, including assessment of the central model of Alzheimer's disease (AD) pathogenesis, according to which TAU pathology begins focally but expands catastrophically under the influence of amyloid-β (Aβ) pathology to mediate neurodegeneration and cognitive decline. Initial TAU deposition occurs many years before Aβ in a specific area of the medial temporal lobe. Building on recent work that enabled focus of molecular PET measurements on specific TAU-vulnerable convolutional temporal lobe anatomy, we applied an automated anatomic sampling method to quantify TAU PET signal in 443 adult participants from several observational studies of aging and AD, spanning a wide range of ages, Aβ burdens, and degrees of clinical impairment. We detected initial cortical emergence of tauopathy near the rhinal sulcus in clinically normal people and, in a subset with longitudinal 2-year follow-up data (n = 104), tracked Aβ-associated spread of TAU from this site first to nearby neocortex of the temporal lobe and then to extratemporal regions. Greater rate of TAU spread was associated with baseline measures of both global Aβ burden and medial temporal lobe TAU. These findings are consistent with clinicopathological correlation studies of Alzheimer's tauopathy and enable precise tracking of AD-related TAU progression for natural history studies and prevention therapeutic trials.