PECULIARITIES OF MORPHO-ANATOMICAL STRUCTURE OF GYPSYWORT (LYCOPUS EUROPAEUS L.) IN RESPONSE TO TREATMENT WITH SILICON-BASED MICROFERTILIZER

Abstract

Background. For the comprehension the mechanisms of adaptation and increasing the bioproductivity of the Gypsywort, at the level of variability of micromorphological and anatomical features of the epidermis of the leaf plate and stem, it is proposed to study the reactions of plants to the use of micronutrients with adaptagenic properties.
 Scientific originality. For the first time in the conditions of crop rotation, the adaptogenic effect of the micro-fertilizer Siliplant on the plants of the Gypsywort at the degree of the epidermal structures of the leaf and stem was shown and confirmed.
 This work aim is to identify the morpho-anatomical features of Gypsywort, emerged as the result of exogenous treatment of plants with microfertilizer Siliplant.
 Materials and methods. The Gypsywort’s plants, growing in the conditions of field crop rotation at the experimental field, were treated in the budding by micro-fertilizer Siliplant (0.055 gallons per acre). The control variant was treated with water. The consumption of the solution was 32,07 gallons per acre. The research material was collected under the second-year plants during the mass flowering phase. Leaf plates of the middle part of the shoots (10 pcs.) were taken from plants (5 pcs. of each variant). The anatomical method of analysis included microscopy and microphotography. A 9x Ernst Zeits Wetzlar light microscope and a 14.0 Mp USB 2.0 C-Mount camera were used for the study. The sample consisted of 10 measurements, statistical processing of the results was performed in Microsoft Excel. For each studied trait, its average value (M), the error of the mean (m) and the coefficient of variation (Cv%) were determined.
 Results. The number and size of stomata were determined, the classification of trichomes was refined, their distribution, the density of pubescence and the number of essential oil glands were studied explored depending on the treatments of plants with Si-based microfertilizer. It has been found out that in plants growing in the field practically do not have hydathodes and pubescence increases.
 Conclusion. Treatment of plants with silicon Si-based microfertilizer contributes to their adaptation to xeromorphic conditions: pubescence decreases, the number of essential oil glands increases, the growth of the bark and xylem elements intensifies. The increased number of stomates and their sizes in the variant with silicon-containing microfertilizer, compared to the control samples indicates its anti-stress effect on the gypsywort plants. The established features of the anatomical structure of plants can also be important in determining the authenticity of medicinal raw materials of Lycopus europaeus L. plants during microscopic analysis.