Analytical Surface Research Articles

Rotational Mode-Specificity in the Cl + C2H6 → HCl + C2H5 Reaction.

We perform rotational mode-specific quasi-classical trajectory simulations using a high-quality ab initio analytical potential energy surface for the Cl(2P3/2) + C2H6 → HCl + C2H5 reaction. As ethane, being a prolate-type symmetric top, can be characterized by the J and K rotational quantum numbers, the excitation of two rotational modes, the tumbling (J, K = 0) and spinning (J, K = J) rotations of the reactant is carried out with J = 10, 20, 30, and 40 at a wide range of collision energies. The impacts of rotational excitation on the reactivity, the mechanism, and the post-reaction distribution of energy are investigated: (1) exciting both rotational modes enhances the reactivity with the spinning rotation being more effective due to its coupling to the C–H stretching vibrational normal modes (C–H bond elongating effect) and larger rotational energies, (2) rotational excitation increases the dominance of direct rebound over the stripping mechanism, while collision energy favors the latter, (3) investing energy in tumbling rotation excites the translational motion of the products, while the excess spinning rotational energy readily flows into the internal degrees of freedom of the ethyl radical or, less significantly, into the HCl vibration, probably due to the pronounced rovibrational coupling in this case. We also study the relative efficiency of vibrational and rotational excitation on the reactivity of the barrierless and thus translationally hindered title reaction.

Accuracy and convergence of the curvature and normal vector discretizations for 3D static and dynamic front-tracking interfaces

In this work, three classes of numerical methods are investigated to evaluate the mean curvature, the unit normal vector and the surface tension on a front tracking interface encountered in the simulation of multiphase flows with separated phases. The Laplace-Beltrami Operator discretization, the Integral Formulation of the surface tension and the Surface Reconstruction technique are well-known methods used in the literature, but whose accuracy, robustness and convergence properties are seldom studied. In a first step, different variants of these methods are presented and compared against each other on a static analytical surface to measure their sensitivity to the size and regularity of the mesh. Then, to assess the influence of the surface advection scheme and the remeshing procedures, two original and time dependent analytical surfaces have been developed, leading to a ligament formation or the birth of a drop/bubble on a flat surface/liquid film. Comparisons to such dynamical surfaces are especially useful and significant, since they highlight the sensitivity of numerical methods for the interface property calculation to the errors produced by the Lagrangian transport of the front-tracking surface. Finally, the accuracy of the different approximations is correlated to their computation time, so that the user may choose the most appropriate method according to the desired accuracy and cost.

Performance analyses of mesh-based local Finite Element Method and meshless global RBF Collocation Method for solving Poisson and Stokes equations

Steady and unsteady Poisson and Stokes equations are solved using mesh dependent Finite Element Method and meshless Radial Basis Function Collocation Method to compare the performances of these two numerical techniques across several criteria. The accuracy of Radial Basis Function Collocation Method with multiquadrics is enhanced by implementing a shape parameter optimization algorithm. For the time-dependent problems, time discretization is done using Backward Euler Method. The performances are assessed over the accuracy, runtime, condition number, and ease of implementation. Three error kinds considered; least square error, root mean square error and maximum relative error. To calculate the least square error using meshless Radial Basis Function Collocation Method, a novel technique is implemented. Imaginary numerical solution surfaces are created, then the volume between those imaginary surfaces and the analytic solution surfaces is calculated, ensuring a fair error calculation. Lastly, all results are put together and trends are observed. The change in runtime vs. accuracy and number of nodes; and the change in accuracy vs. the number of nodes is analyzed. The study indicates the criteria under which Finite Element Method performs better and conditions when Radial Basis Function Collocation Method outperforms its mesh dependent counterpart.

Composite Peptide-Agarose Hydrogels for Robust and High-Sensitivity 3D Immunoassays.

Canonical immunoassays rely on highly sensitive and specific capturing of circulating biomarkers by interacting biomolecular baits. In this frame, bioprobe immobilization in spatially discrete three-dimensional (3D) spots onto analytical surfaces by hydrogel encapsulation was shown to provide relevant advantages over conventional two-dimensional (2D) platforms. Yet, the broad application of 3D systems is still hampered by hurdles in matching their straightforward fabrication with optimal functional properties. Herein, we report on a composite hydrogel obtained by combining a self-assembling peptide (namely, Q3 peptide) with low-temperature gelling agarose that is proved to have simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays. We demonstrate the real-case scenario feasibility of our 3D system in the profiling of Covid-19 patients' serum IgG immunoreactivity, which showed remarkably improved signal-to-noise ratio over canonical assays in the 2D format and exquisite specificity. Overall, the new two-component hydrogel widens the perspectives of hydrogel-based arrays and represents a step forward towards their routine use in analytical practices.

Multiplicity, regularity and blow-spherical equivalence of real analytic sets

This article is devoted to studying multiplicity and regularity of analytic sets. We present an equivalence for analytic sets, named blow-spherical equivalence, which generalizes differential equivalence and subanalytic bi-Lipschitz equivalence and, with this approach, we obtain several applications on analytic sets. On multiplicity, we present a generalization for Gau–Lipman’s Theorem about differential invariance of the multiplicity in the complex and real cases, and we show that the multiplicity \(\mathrm{mod}\,2\) is invariant under blow-spherical homeomorphisms in the case of real analytic curves and surfaces and also for a class of real analytic foliations and is invariant by (image) arc-analytic blow-spherical homeomorphisms in the case of real analytic hypersurfaces, generalizing some results proved by G. Valette. On regularity, we show that blow-spherical regularity of real analytic sets implies \(C^1\) smoothness only in the case of real analytic curves. We present also a complete classification of the germs of real analytic curves.

Full-dimensional potential energy surface for the H + CH3OH reaction. Theoretical kinetics and dynamics study.

The dynamics and kinetics of the abstraction reactions of hydrogen atoms with methanol have been studied using quasi-classical trajectory calculations and variational transition state theory with tunnelling corrections, based on a new analytical potential energy surface (PES). The new PES is a valence-bond/molecular mechanics (VB/MM) expression that provides us with the potential energy for any set of Cartesian coordinates. Two reaction channels are considered: hydrogen abstraction from the methyl group (R1) and hydrogen abstraction from the alcohol group (R2), R1 being much more likely to occur in the wide temperature range under study (250-1000 K), as expected from the lower barrier height. Our dynamic calculations at a collision energy of 20 kcal mol-1 show that the H2 co-product is produced mainly in its vibrational ground-state and little rotation excitation is found. As for our kinetic results, they agree with those from previous theoretical studies as well as with those from kinetic experimental results (rate constants and kinetic isotopic effects), lending confidence to the analytical PES presented here. Thus, we expect this PES to be a simple yet powerful tool to understand such an important reaction in combustion chemistry at very high temperatures and interstellar chemistry at very low temperatures.

Automated full-dimensional potential energy surface development and quasi-classical dynamics for the HI(X1Σ+) + C2H5 → I(2P3/2) + C2H6 reaction.

A full-dimensional spin-orbit-corrected analytical coupled-cluster-quality potential energy surface (PES) is developed for the HI(X1Σ+) + C2H5 → I(2P3/2) + C2H6 reaction using the ROBOSURFER program package, and a quasi-classical trajectory (QCT) study on the new PES is reported. The stationary-point relative energies obtained on the PES reproduce well the benchmark values. Our simulations show that in the 0.5-40 kcal mol-1 collision energy (Ecoll) range, the b = 0 reaction probability, where b denotes the impact parameter, increases first and then stays steady with increasing Ecoll, reaching around 10% when Ecoll = 5 kcal mol-1. No significant Ecoll dependence is observed in the range of 5-40 kcal mol-1. The reaction probabilities decrease monotonically with increasing b, and the maximum b where the reactivity vanishes becomes smaller and smaller as Ecoll increases. Scattering angle distributions show a forward scattering preference, indicating the dominance of the direct stripping mechanism, which is more obvious than in the case of HBr + C2H5 → Br + C2H6. The reaction clearly favors H-side attack over side-on HI and the least-preferred I-side approach, and favors side-on CH3CH2 attack marginally over CH2-side and the least-preferred CH3-side approach at high Ecoll. At low Ecoll, however, the dominant effect of H-side attack becomes weaker, while the side-on CH3CH2 attack becomes comparable with CH2-side and the former is a little less favored when Ecoll = 0.5 kcal mol-1. It turns out that the initial translational energy is converted mostly into product recoil, whereas the reaction energy excites the C2H6 vibration. The vibrational and rotational distributions of the C2H6 product slightly blue-shift as Ecoll increases, and none of the reactive trajectories violates the zero-point energy (ZPE) constraint. The energy transfer in the HI + C2H5 → I + C2H6 reaction is very similar to the case in the HBr + C2H5 → Br + C2H6 system that we investigated recently.

Full-dimensional potential energy surface development and dynamics for the HBr + C2H5 → Br(2P3/2) + C2H6 reaction.

We report a full-dimensional spin-orbit-corrected analytical potential energy surface (PES) for the HBr + C2H5 → Br + C2H6 reaction and a quasi-classical dynamics study on the new PES. For the PES development, the ROBOSURFER program package is applied and the ManyHF-based UCCSD(T)-F12a/cc-pVDZ-F12(-PP) energy points are fitted using the permutationally-invariant monomial symmetrization approach. The spin-orbit coupling at the level of MRCI-F12+Q(5,3)/cc-pVDZ-F12(-PP) is taken into account, since it has a significant effect in the exit channel of this reaction. Our simulations show that in the 1-40 kcal mol-1 collision energy (Ecoll) range the b = 0 reaction probability increases first and then decreases with increasing Ecoll, reaching around 15% at the medium Ecoll. No significant Ecoll dependence is observed in the range of 5-20 kcal mol-1. The reaction probabilities decrease monotonically with increasing b and the maximum b where reactivity vanishes is smaller and smaller as Ecoll increases. Unlike in the case of HBr + CH3, the integral cross-section decays sharply as Ecoll changes from 5 to 1 kcal mol-1. Scattering angle distributions usually show forward scattering preference, indicating the dominance of the direct stripping mechanism. The reaction clearly favors H-side attack over side-on HBr and the least-preferred Br-side approach, and favors side-on CH3CH2 attack over the CH2-side and the least-preferred CH3-side approach. The initial translational energy turns out to convert mostly into product recoil, whereas the reaction energy excites the C2H6 vibration. The vibrational and rotational distributions of the C2H6 product slightly blue-shift as Ecoll increases, and very few reactive trajectories violate zero-point energy.

Vibrational mode-specific dynamics of the F- + CH3CH2Cl multi-channel reaction.

We investigate the mode-specific dynamics of the ground-state, C-Cl stretching (v10), CH2 wagging (v7), sym-CH2 stretching (v1), and sym-CH3 stretching (v3) excited F- + CH3CH2Cl(vk = 0, 1) [k = 10, 7, 1, 3] → Cl- + CH3CH2F (SN2), HF + CH3CHCl-, FH⋯Cl- + C2H4, and Cl- + HF + C2H4 (E2) reactions using a full-dimensional high-level analytical global potential energy surface and the quasi-classical trajectory method. Excitation of the C-Cl stretching, CH2 stretching, and CH2/CH3 stretching modes enhances the SN2, proton abstraction, and FH⋯Cl- and E2 channels, respectively. Anti-E2 dominates over syn-E2 (kinetic anti-E2 preference) and the thermodynamically-favored SN2 (wider reactive anti-E2 attack angle range). The direct (a) SN2, (b) proton abstraction, (c) FH⋯Cl- + C2H4, (d) syn-E2, and (e) anti-E2 channels proceed with (a) back-side/backward, (b) isotropic/forward, (c) side-on/forward, (d) front-side/forward, and (e) back-side/forward attack/scattering, respectively. The HF products are vibrationally cold, especially for proton abstraction, and their rotational excitation increases for proton abstraction, anti-E2, and syn-E2, in order. Product internal-energy and mode-specific vibrational distributions show that CH3CH2F is internally hot with significant C-F stretching and CH2 wagging excitations, whereas C2H4 is colder. One-dimensional Gaussian binning technique is proved to solve the normal mode analysis failure caused by methyl internal rotation.

Analytical surfaces for ship hulls

The choice of optimal shape of ship hull surface is one of the main problems of ship architects and designs. A choice of the form is based on empirical formulae or on intuition of designers. In the article a method of determination of explicit algebraic equations of theoretical shape of ship hull with three main cross-sections given in advance and coinciding with the design waterline, the midship section, and with the main buttock line is given. The forms of the lines in the main cross-sections are chosen from conditions taken in advance. These surfaces are called hydrodynamic. A method is illustrated for three threes of main cross-sections of the ship hulls, i.e. nine hydrodynamic surfaces were constructed. All algebraic equations were converted to parametrical form for comfort of computer modelling. With their help, all nine ship surfaces proposed for the introduction were visualized. Having changed constants containing in the surface equations, i.e. correcting the forms of three main geometric parameters of ship hull, one can select the most rational shape of hull surface for the first approach. Further, it is possible to begin planning parallel middle bodies or to combine bow and stern extremities of a ship from different fragments of algebraic surfaces but with the same midship sections. In a paper, only geometrical problems of design of theoretical hull shape are described.

Influence of the concrete strength and the type of supports on the stress-strain state of a hyperbolic paraboloid shell footbridge structure

Relevance. This work is the first in a series of publications on the selection of a suitable analytical surface for implementation as a self-supporting structure for a thin shell footbridge. The study on the influence of concrete strength, live load position and support types on the stress-strain state of a hyperbolic paraboloid (hypar) shell is presented. Objective - to define the initial design parameters such as the appropriate concrete strength and the support type that generates the best structural behaviour to perform the subsequent structural design of a thin shell footbridge. Methods. The static finite element analysis was performed for 4 compressive strengths of concrete (28, 40, 80, 120 MPa) which correspond normal, high and ultra-high resistance concrete, 5 different live load arrangements and 3 different support conditions. Results. The shell model with pinned (two-hinged) supports shows the same vertical displacements as the model with fixed supports (hingeless). For the studied shell thickness, in terms of stress behaviour, the model with pinned ends is more efficient. The combination of two-hinged supports with 80 MPa concrete strength shows a better structural performance.

Parametric modeling compressed ellipsoid of rotation as the analytic surface

The article deals with the shaping of the analytical surface in the form of a compressed ellipsoid of rotation. In the process of research, the principle of the golden section was applied, an ellipsoid of rotation with optimal parameters for the design of a large-span unique building of the exhibition complex was obtained. To solve the problem of choosing a rational analytical surface in the Lira-CAD software package, three variants of constructive solutions for the frame of the exhibition complex have been developed. The finite element method is used, the most popular numerical calculation method for studying the stress-strain state of large-span buildings and structures. The analysis of the calculation results made it possible to choose the optimal variant of the structural solutions of the building frame. The check of the dynamic characteristics of the proposed structural solution of the building frame is carried out. The obtained results of the dynamic calculation showed the correctness and efficiency of the adopted structural solutions of the building frame.

Mechanistic understanding of the sensing process by analyzing response curves of TiO2 based humidity sensors

Sensors function by interacting with an appropriate stimulus, undergo a change in property, which is then diagnosed by making some measurements. For any sensor, the type of interaction between analyte and sensor surface determines its overall performance. This article explores the philosophy in which primary measurements like response curves can hold information on the “type” of interaction occurring between analyte and sensing material. As case study, titanium oxide (TiO2) pellet sensors fabricated by sol-gel growth of TiO2 nanoparticles (as-grown and annealed) are investigated for humidity sensing at room temperature. The sensors display a very fast response in the 0%–30% relative humidity (%RH) range and return to their initial state without applying any external heat treatment. The response curves are analysed in view of adsorption processes guided by Langmuir isotherms. Correlation between sensor microstructure, adsorption processes and response curve is used to build the mechanistic understanding of the sensing process. The results bring out a unique correlation between sensor microstructure, interaction of analyte with sensing material and profile of response curves. Further, the synthesised sensors exhibit a linear response in the 0%–30% RH range making them suitable for low humidity environments like food packaging industry.

Digital models of parametric structures

Formation of parametric structure digital model are considered. Investigation was based on the analysis of the shaping evolution of the analytical surface in the form of a hyperboloid of rotation. Finite element frame represents a spatial plate-rod system has been developed during creation of the digital model. This scheme allows to study the effectiveness of constructive solutions under dynamic influence at the design stage. Dynamic characteristics of various design solutions are considered in process of choosing a rational variant of the building frame. The influence of the inclusion of outrigger floor in the model of the frame at various levels of the building are investigated. The proposed method of research and application of the possibilities of the shaping evolution allows to proceed to the modernization of BIM technologies in the design of unique buildings. Recommendations for refining the algorithm for studying parametric object digital models were developed.

Oxidative Cleavage-Based Three-Dimensional DNA Biosensor for Ratiometric Detection of Hypochlorous Acid and Myeloperoxidase.

Methods to detect and quantify disease biomarkers with high specificity and sensitivity in biological fluids play a key role in enabling clinical diagnosis, including point-of-care testing. Myeloperoxidase (MPO) is an emerging biomarker for the detection of inflammation, neurodegenerative diseases, and cardiovascular disease, where excess MPO can lead to oxidative damage to biomolecules in homeostatic systems. While numerous methods have been developed for MPO analysis, most techniques are challenging in clinical applications due to the lack of amplification methods, high cost, or other practical drawbacks. Enzyme-linked immunosorbent assays are currently used for the quantification of MPO in clinical practice, which is often limited by the availability of antibodies with high affinity and specificity and the significant nonspecific binding of antibodies to the analytical surface. In contrast, nucleic acid-based biosensors are of interest because of their simplicity, fast response time, low cost, high sensitivity, and low background signal, but detection targets are limited to nucleic acids and non-nucleic acid biomarkers are rare. Recent studies reveal that the modification of a genome in the form of phosphorothioate is specifically sensitive to the oxidative effects of the MPO/H2O2/Cl- system. We developed an oxidative cleavage-based three-dimensional DNA biosensor for rapid, ratiometric detection of HOCl and MPO in a "one-pot" method, which is simple, stable, sensitive, specific, and time-saving and does not require a complex reaction process, such as PCR and enzyme involvement. The constructed biosensor has also been successfully used for MPO detection in complex samples. This strategy is therefore of great value in disease diagnosis and biomedical research.

Thermal stability of surface oxides on nickel alloys (NiCr and NiCrMo) investigated by XPS and ToF-SIMS

Thermal stability of surface oxides on Ni-20Cr and Ni-20Cr-10Mo alloys, including air formed native oxides and passive films formed by electrochemical passivation, was investigated by stepwise heat treatments in ultra-high vacuum up to 300 °C. The modifications of the surface oxides were analyzed by XPS and in situ ToF-SIMS. The analytical surface characterization at the nanoscale revealed modifications of the protective surface oxides that depend on temperature and nature of the alloy. A loss of Ni(OH)2 in the outer hydroxide layer and a decrease of film thickness are observed on both alloys above 100 °C. At 200 °C, Ni hydroxide in the outer layer is no longer stable while MoO3 decreases and MoO2 increases in the oxide film. At 300 °C no native oxide film remains at the surface of Ni-20Cr alloy, whereas a thin Cr oxide film partially covering the surface remains on passivated Ni-20Cr and for both native and passivated surfaces of Ni-20Cr-10Mo alloy. It is shown that the thermal stability of the surface oxide is significantly improved by electrochemical passivation on both alloys and is further enhanced by the presence of Mo in the oxide on the Mo-containing alloy. Such findings are useful for the development of corrosion protection strategies.

Bi2WO6/NH2-MIL-88B(Fe) heterostructure: An efficient sunlight driven photocatalyst for the degradation of antibiotic tetracycline in aqueous medium

The development of highly efficient sunlight assisted photocatalysts has been acknowledged as a promising strategy for the enhanced degradation of antibiotics. In this work, effectual fabrication of a novel Bi2WO6/NH2-MIL-88B(Fe) heterostructure was carried through solvothermal route. The structural, morphological and compositional analysis was done by employing number of analytical techniques, namely XRD, FTIR, HRTEM, FESEM, XPS, PL and BET surface area. The prepared Bi2WO6/NH2-MIL-88B(Fe) heterostructure was utilized as an efficient photocatalyst towards decomposition of a typical antibiotic tetracycline (TC) in aqueous medium. It was found that Bi2WO6/NH2-MIL-88B(Fe) heterostructure exhibited improved degradation efficiency of about 89.4% within 130 min of solar illumination than pristine NH2-MIL-88B(Fe) under optimized parameters i.e. initial drug solution of 10 mg/L concentration at pH 4 with 0.35 g/L dose of catalyst. Moreover, adsorption studies, kinetics and isotherms of adsorption on TC were also investigated. Results revealed that adsorption kinetics followed pseudo 2nd order model and isotherm data fitted well with Freundlich model (R2 = 0.99803) as compared to Temkin and Langmuir. The ameliorating photocatalytic capability could be primarily accredited to the heterojunction created among Bi2WO6 and NH2-MIL-88B(Fe) which facilitated the charge transfer and thus determines high catalytic efficiency. The enhanced photocatalyic effect was further verified by electrochemical impedance and photocurrent studies. The prepared composite also exhibited longer carrier lifetime (140.72 ns) compared to pure MOF (132.05 ns) and Bi2WO6 (136.39 ns). Further, based on the radical trapping investigations, role of superoxide radicals was dominant and detailed mechanism was proposed for the photocatalytic degradation process. The major intermediates formed during the course of reaction were also examined using LCMS analysis. The photodegradation was also carried over simulated hospital wastewater by the prepared heterostructure and 60.5% TOC was obtained under solar light in 390 min. Moreover, the synthesized heterostructure showed good recyclability up to three cycles depicting good stability.

An implicit analytical surface potential based model for long channel symmetric double-gate MOSFETs accounting for oxide and interface trapped charges

We present analytical models for calculating surface potential for both undoped (intrinsic) and doped (extrinsic) long channel symmetric double-gate MOSFETs. Starting with Poisson’s equation, we derive the implicit surface potential equation(s) for the symmetric double-gate MOSFET in the accumulation and depletion/inversion regions of operation. This results in a continuous surface potential curve throughout all operating regions of the device. By introducing a defect potential to surface potential equations for the symmetric double-gate MOSFET, the models add the effects of oxide-trapped charge and interface traps (both uniform and non-uniform distributions) that can build up in the gate dielectric of the device. A drain current model is presented for both the undoped and doped cases of the SDG MOSFET. The accuracy of the surface potential analytical models and drain current models are validated by comparing to numerical results from two dimensional TCAD simulations.

Vibrational mode-specific dynamics of the F(2P3/2) + C2H6 → HF + C2H5 reaction.

We investigate the competing effect of vibrational and translational excitation and the validity of the Polanyi rules in the early- and negative-barrier F(2P3/2) + C2H6 → HF + C2H5 reaction by performing quasi-classical dynamics simulations on a recently developed full-dimensional multi-reference analytical potential energy surface. The effect of five normal-mode excitations of ethane on the reactivity, the mechanism, and the post-reaction energy flow is followed through a wide range of collision energies. Promoting effects of vibrational excitations and interaction time, related to the slightly submerged barrier, are found to be suppressed by the early-barrier-induced translational enhancement, in contrast to the slightly late-barrier Cl + C2H6 reaction. The excess vibrational energy mostly converts into ethyl internal excitation while collision energy is transformed into product separation. The substantial reaction energy excites the HF vibration, which tends to show mode-specificity and translational energy dependence as well. With increasing collision energy, direct stripping becomes dominant over the direct rebound and indirect mechanisms, being basically independent of reactant excitation.

Insight into the Influence of Analyte Molecular Structure Targeted on MoS2-GO-Coated Electrochemical Nanosensors.

MoS2-GO composites were fabricated by an ultrasonication method at room temperature. Raman spectra, emission scanning electron microscopy (SEM), and transmission electron microscopy (TEM) images were used to study the structural characteristics, morphologies, and sizes of the synthesized materials. An MoS2-GO/SPE (screen-printed electrode) was prepared by a facile dropping method and acted as an effective electrochemical sensor toward clenbuterol (CLB) and 4-nitrophenol (4-NP) detection. Based on the obtained results, the influence of analyte molecular structure on the adsorption ability and electronic interoperability between the targeted analyte and electrode surface were investigated in detail and discussed as well, through some electrochemical kinetic parameters (electron/proton-transfer number, electron transfer rate constant (ks), charge transfer coefficient, and adsorption capacity (Γ)). In particular, it should be stressed that 4-NP molecules possess a simple molecular structure with many positive effects (electronic, conjugation, and small steric effects) and flexible functional groups, resulting in fast electron transport/charge diffusion and effective adsorption process as well as strong interactions with the electrode surface. Therefore, 4-NP molecules have been facilitated better in electrochemical reactions at the electrode surface and electrode-electrolyte interfaces, leading to improved current response and electrochemical sensing performance, compared with those of CLB.