Mapping knowledge domain (MKD) is an important application of visualization technology in Bibliometrics, which has been extensively applied in psychology, medicine, and information science. In this paper we conduct a systematic analysis of the development trend on road safety studies based on the Science Citation Index Expanded (SCIE) and Social Sciences Citation Index (SSCI) articles published between 2000 and 2018 using the MKD software tools VOSviewer and Sci2 Tool. Based on our analysis, we first present the annual numbers of articles, origin countries, main research organizations and groups as well as the source journals on road safety studies. We then report the collaborations among the main research organizations and groups using co-authorship analysis. Furthermore, we adopt the document co-citation analysis, keywords co-occurrence analysis, and burst detection analysis to visually explore the knowledge bases, topic distribution, research fronts and research trends on road safety studies. The proposed approach based on the visualized analysis of MKD can be used to establish a reference information and research basis for the application and development of methods in the domain of road safety studies. In particular, our results show that the knowledge bases (classical documents) of road safety studies in the last two decades have focused on five major areas of “Crash Frequency Data Analysis”, “Driver Behavior Questionnaire”, “Safety in Numbers for Walkers and Bicyclists”, “Road Traffic Injury and Prevention”, and “Driving Speed and Road Crashes”. Among the research topics, the five dominant clusters are “Causation and Injury Severity Analysis of Road Accidents”, “Epidemiologic Study and Prevention of Road Traffic Injury”, “Intelligent Transportation System and Active Safety”, “Young drivers’ driving behavior and psychology”, and “Older drivers’ psychological and physiological characteristics”. Finally, the burst keywords in research trends include Cycling, Intelligent Transportation Systems, and Distraction.