Reaction Analysis Research Articles

Enhancing surface performance of wool using reduced ionic liquid

Wool surface modification is essential for its application in the textile industry. In this work, a novel approach using reduced ionic liquid (IL) was applied to modify the surface of wool and enhance surface-related properties of wool for overcoming the unsustainable drawbacks in the present methods. The results from the surface morphology indicated that the wool was gradually dissolved from the outside to the inside of the fiber. The treated wool was characterized using the X-ray photoelectron spectroscopy (XPS), fluorescence microscopy and the Allwörden reaction. Moreover, felting shrinkage, mechanical properties, and dyeing properties of the treated wool were analyzed. Disulfide bonds were cleaved during the process from XPS test. The Allwörden reaction and fluorescence analysis showed damaged scale layer on the wool surface and an intact fiber main body with an acceptable loss of mechanical properties. Compared with the untreated wool, the anti-felting properties and dyeing performance improved due to the cleavage of disulfide bonds and the removal of the lipid layer, indicating that the treatment could modify the wool surface. Overall, the study supports a novel, facile and highly efficient approach for preparing a value-adding natural protein fiber with a potential for large-scale production.

Investigation of Multisensory Harmonic Priming: Audiovisual Integration in Chord Perception

ABSTRACT Although typically thought of as a unimodal phenomenon, musical experience is fundamentally multisensory. The current study examined such multisensory influences by exploring the impact of visual information on the processing of higher-order musical structure, within the context of harmonic expectancy formation. Specifically, musically trained participants experienced four different priming events, including an auditory chord prime, a visual chord prime, an auditory-visual note/chord prime, and an auditory note prime, and discriminated harmonically related versus unrelated in-tune and out-of-tune target chords. Analyses of reactions and error rates revealed typical harmonic priming effects (i.e., faster reaction times and lower error rates to related than unrelated targets for in-tune chords) for auditory chord and auditory-visual note/chord primes, but no differences for visual chord and auditory note primes. The finding that auditory-visual note/chord primes led to priming indicates that visual information can induce auditory expectancies when supported by auditory cue information. These results are discussed within the context of models of tonal relations, as well as in relation to theories of multisensory processing in general.

Detection of Pestivirus in small ruminants in Central Java, Indonesia.

Background and Aim:Globally, pestiviruses are among the most economically important viral pathogens of livestock. The genus Pestivirus comprises four species, including bovine viral diarrhea virus type 1 and 2 (BVDV-1 and BVDV-2), which infect cattle, border disease virus and classical swine fever virus which infect small ruminants and pigs, respectively. Accumulating evidence suggests that pestiviruses are no longer species-specific, creating new challenges for disease control. In Indonesia, investigations related to pestiviruses remain focused on cattle as the primary host and no research has been conducted on small ruminants (sheep and goats). Therefore, the present study aimed to study the possible occurrence of pestivirus (BVDV or BVD) infections in small ruminants in Indonesia, particularly in Central Java.Materials and Methods:We used 46 blood samples consisting of 26 sheep’s blood and 20 goat’s blood. Samples were selected from 247 small ruminant blood collected between July and October 2020 in Central Java, Indonesia, which met the following criteria: Female, local species, approximately 1-2 years old, never been pregnant, raised in the backyard, and had no close contact with cattle in either shelter or grazing area. We tested plasma samples from sheep and goats using competitive antibody enzyme-linked immunosorbent assay to detect specific antibodies against pestivirus followed by reverse transcription-polymerase chain reaction (RT-PCR) analysis for all positive samples to differentiate the species of pestivirus.Results:Two of the 20 samples collected from goats were positive for pestivirus at the serological and molecular levels, whereas 2 of 26 samples collected from sheep were doubtful but tested negative by RT-PCR. The genotyping test results obtained using nested PCR revealed that the positive samples collected from goats had a BVDV-1 genotype.Conclusion:The results of the present study demonstrated that BVDV-1 can infect species other than bovines, in Central Java, Indonesia. Further studies involving a larger number of samples are required to: (1) Determine the actual seroprevalence of pestiviruses in small ruminants and (2) Determine the potency of small ruminants as reservoirs for pestiviruses, both of which are important for the identification of the appropriate control program for pestiviruses in Indonesia.

Dried Blood Spot Screening System for Spinal Muscular Atrophy with Allele-Specific Polymerase Chain Reaction and Melting Peak Analysis

Background and Aim: Spinal muscular atrophy (SMA) is a lower motor neuron disease with autosomal recessive inheritance caused by homozygous SMN1 deletions. Although SMA has been considered as incurable, newly developed drugs improve life prognoses and motor functions of patients. To maximize the efficacy of the drugs, SMA patients should be treated before symptoms become apparent. Thus, newborn screening for SMA is strongly recommended. In this study, we aim to establish a new simple screening system based on DNA melting peak analysis. Materials and Methods: A total of 124 dried blood spot (DBS) on FTA® ELUTE cards (51 SMN1-deleted patients with SMA, 20 carriers, and 53 controls) were punched and subjected to direct amplification of SMN1 and CFTR (reference gene). Melting peak analyses were performed to detect SMN1 deletions from DBS samples. Results: A combination of allele-specific polymerase chain reaction (PCR) and melting peak analyses clearly distinguished the DBS samples with and without SMN1. Compared with the results of fresh blood samples, our new system yielded 100% sensitivity and specificity. The advantages of our system include (1) biosafe collection, transfer, and storage for DBS samples, (2) obviating the need for DNA extraction from DBS preventing contamination, (3) preclusion of fluorescent probes leading to low PCR cost, and (4) fast and high-throughput screening for SMN1 deletions. Conclusion: We demonstrate that our system would be applicable to a real-world newborn screening program for SMA, because our new technology is efficient for use in routine clinical laboratories that do not have highly advanced PCR instruments.

Experimental Investigation of the Combustion Behavior of Single-Nozzle Liquid-FLOX®-Based Burners on an Atmospheric Test Rig

Abstract As an alternative to the commonly used swirl burners in microgas turbines (MGT), the FLOX®-based combustion concept promises great potential for the nitric oxide emission reduction and increased fuel flexibility. Despite having to deal with a new set of challenges while utilizing liquid fuel in the burner, first steps are taken to gain more information on the influencing operational parameters. In this regard, a FLOX®-based liquid fuel burner is developed to fit into a newly designed combustor for the Capstone C30 MGT. The C30 combustor operates with three burners arranged tangentially to an annular combustion chamber and provides a total thermal power of 115 kW. In this work, operational properties of merely one of the three C30 liquid fuel burners are investigated and the rest of the two burners are emulated in form of hot cross-flow. As for the liquid burners, the experiments are conducted with three geometrically different single-nozzle burners at atmospheric pressure. The cross-flow is realized by utilizing a 20–nozzle FLOX®-based natural gas combustor. Measurements include visualization of the reaction zone and analysis of the exhaust gas emissions. By detecting the hydroxyl radical chemiluminescence (OH*-CL) emissions, the position of the heat release zone within the combustion chamber is attained. Correspondingly, the flame height above burner and the flame length are calculated. The investigated design parameters include air preheat temperature up to 733 K, equivalence ratio, burner geometry, and thermal power. Through variation of thermal power, the effect of liquid fuel preparation, i.e., atomization, evaporation, and mixing on combustion properties and exhaust gas emissions are examined. The results show that the burners with the medium diameter consistently performed remarkably at different flame temperatures and thermal powers. The lowest NOx and CO emissions for the medium diameter burner lied between 5 to 7 ppm and 8 to 10 ppm, respectively.

Unexpectedly enhanced α -particle preformation in Ti48 probed by the ( p,pα ) reaction

The formation of $\alpha$ particle on nuclear surface has been a fundamental problem since the early age of nuclear physics. It strongly affects the $\alpha$ decay lifetime of heavy and superheavy elements, level scheme of light nuclei, and the synthesis of the elements in stars. However, the $\alpha$-particle formation in medium-mass nuclei has been poorly known despite its importance. Here, based on the $^{48}{\rm Ti}(p,p\alpha)^{44}{\rm Ca}$ reaction analysis, we report that the $\alpha$-particle formation in a medium-mass nucleus $^{48}{\rm Ti}$ is much stronger than that expected from a mean-field approximation, and the estimated average distance between $\alpha$ particle and the residue is as large as 4.5 fm. This new result poses a challenge of describing four nucleon correlations by microscopic nuclear models.

MiR-96 Inhibits SV2C to Promote Depression-Like Behavior and Memory Disorders in Mice.

Accumulating evidence continues to emphasize the role of microRNAs as significant contributors to depression-like behavior and memory disorders. The current study aimed to investigate the mechanism by which miR-96 influences depression-like behavior and memory deficit in mice. A depression-like behavior and memory disorder mouse model was initially established by means of intraperitoneal injection with lipopolysaccharide. Memory deficits in the mice were evaluated using the Novel Object Recognition Test and Morris water maze experiments, whereas the Sucrose Preference Experiment and forced swimming experiments were performed to identify depression-like behavior in mice. The levels of tumor necrosis factor-α, malondialdehyde, superoxide dismutase, glutathione, and the monoamine transmitters 5-hydroxytryptamine and dopamine were subsequently detected in the serum. Reverse transcription-quantitative polymerase chain reaction and Western blot analysis evaluated the expression of miR-96 and SV2C expression in the CA1 hippocampal region of the mice. Finally, the relationship of miR-96 and SV2C was verified by dual-luciferase reporter gene assay. Our data indicated that the expression of miR-96 was increased, whereas that of SV2C was decreased in the CA1 region of mice exhibiting depression-like behavior and memory impairment. When miR-96 was downregulated or SV2C was overexpressed via intra-cerebroventricular injection with a miR-96 antagonist (miR-96 antagomir) or overexpression of SV2C vector, the Novel Object Recognition Test and sucrose preference index were increased, whereas the escape latency, the number of water maze platform crossings, and the immobility time of the mice were decreased. The serum levels of tumor necrosis factor-α, interleukin-1β, and malondialdehyde in the mouse CA1 region of mice were reduced, whereas the levels of superoxide dismutase and glutathione were elevated after the downregulation of miR-96 or overexpression of SV2C. Collectively, our study demonstrates that miR-96 negatively regulates the expression of SV2C, which consequently leads to depression-like behavior and memory impairment in mice. Our findings highlight the potential of miR-96-targeted therapeutics.

The TOMM40 \u2018523\u2019 polymorphism in disease risk and age of symptom onset in two independent cohorts of Parkinson\u2019s disease

Abnormal mitochondrial function is a key process in the pathogenesis of Parkinson’s disease (PD). The central pore-forming protein TOM40 of the mitochondria is encoded by the translocase of outer mitochondrial membrane 40 homologue gene (TOMM40). The highly variant ‘523’ poly-T repeat is associated with age-related cognitive decline and age of onset in Alzheimer’s disease, but whether it plays a role in modifying the risk or clinical course of PD it yet to be elucidated. The TOMM40 ‘523’ allele length was determined in 634 people with PD and 422 healthy controls from an Australian cohort and the Parkinson’s Progression Markers Initiative (PPMI) cohort, using polymerase chain reaction or whole genome sequencing analysis. Genotype and allele frequencies of TOMM40 ‘523’ and APOE ε did not differ significantly between the cohorts. Analyses revealed TOMM40 ‘523’ allele groups were not associated with disease risk, while considering APOE ε genotype. Regression analyses revealed the TOMM40 S/S genotype was associated with a significantly later age of symptom onset in the PPMI PD cohort, but not after correction for covariates, or in the Australian cohort. Whilst variation in the TOMM40 ‘523’ polymorphism was not associated with PD risk, the possibility that it may be a modifying factor for age of symptom onset warrants further investigation in other PD populations.

Using a new video rating tool to crowd-source analysis of behavioural reaction to stimuli

Quantifying the intensity of animals’ reaction to stimuli is notoriously difficult as classic unidimensional measures of responses such as latency or duration of looking can fail to capture the overall strength of behavioural responses. More holistic rating can be useful but have the inherent risks of subjective bias and lack of repeatability. Here, we explored whether crowdsourcing could be used to efficiently and reliably overcome these potential flaws. A total of 396 participants watched online videos of dogs reacting to auditory stimuli and provided 23,248 ratings of the strength of the dogs’ responses from zero (default) to 100 using an online survey form. We found that raters achieved very high inter-rater reliability across multiple datasets (although their responses were affected by their sex, age, and attitude towards animals) and that as few as 10 raters could be used to achieve a reliable result. A linear mixed model applied to PCA components of behaviours discovered that the dogs’ facial expressions and head orientation influenced the strength of behaviour ratings the most. Further linear mixed models showed that that strength of behaviour ratings was moderately correlated to the duration of dogs’ reactions but not to dogs’ reaction latency (from the stimulus onset). This suggests that observers’ ratings captured consistent dimensions of animals’ responses that are not fully represented by more classic unidimensional metrics. Finally, we report that overall participants strongly enjoyed the experience. Thus, we suggest that using crowdsourcing can offer a useful, repeatable tool to assess behavioural intensity in experimental or observational studies where unidimensional coding may miss nuance, or where coding multiple dimensions may be too time-consuming.

CTCF-silenced miR-137 contributes to EMT and radioresistance in esophageal squamous cell carcinoma

BackgroundEsophageal squamous cell carcinoma (ESCC) is one of the most malignant tumors in gastrointestinal system. MicroRNAs (miRNAs) have been reported to be implicated in cancer development. However, the role of miR-137 has not been fully revealed in ESCC.MethodsQuantitative real-time polymerase chain reaction (qRT-PCR) and western blot analyses were separately used to examine RNA level and protein level. 5-ethynyl-2′-deoxyuridine (EdU) assay, transwell assays and flow cytometry analyses were conducted to assess biological behaviors of ESCC cells. Additionally, the interaction between genes were analyzed via Chromatin Immunoprecipitation (ChIP) assay, RNA Binding Protein Immunoprecipitation (RIP) assay, RNA pull down assay and luciferase reporter assay.ResultsMiR-137 was down-regulated in ESCC cells. Upregulation of miR-137 hindered ESCC cell proliferation, migration, invasion and epithelial mesenchymal transition (EMT). Besides, miR-137 enhanced the sensitivity of ESCC cells to irradiation. Moreover, CCCTC-binding factor (CTCF) inactivated miR-137 transcription in ESCC cells. Furthermore, we revealed enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) and paxillin (PXN) as the downstream targets of miR-137. In turn, EZH2 was recruited by CTCF and induced methylation in miR-137 promoter.ConclusionCTCF/Suz12/EZH2 complex-silenced miR-137 facilitates ESCC progression and radioresistance by targeting EZH2 and PXN.

The small molecule ZY-214-4 may reduce the virulence of Staphylococcus aureus by inhibiting pigment production

BackgroundIn recent years, clinical Staphylococcus aureus isolates have become highly resistant to antibiotics, which has raised concerns about the ability to control infections by these organisms. The aim of this study was to clarify the effect of a new small molecule, ZY-214-4 (C19H11BrNO4), on S. aureus pigment production.ResultsAt the concentration of 4 μg/mL, ZY-214-4 exerted a significant inhibitory effect on S. aureus pigment synthesis, without affecting its growth or inducing a toxic effect on the silkworm. An oxidant sensitivity test and a whole-blood killing test indicated that the S. aureus survival rate decreased significantly with ZY-214-4 treatment. Additionally, ZY-214-4 administration significantly reduced the expression of a pigment synthesis-related gene (crtM) and the superoxide dismutase genes (sodA) as determined by real-time quantitative polymerase chain reaction (RT-qPCR) analysis. ZY-214-4 treatment also improved the survival rate of S. aureus-infected silkworm larvae.ConclusionsThe small molecule ZY-214-4 has potential for the prevention of S. aureus infections by reducing the virulence associated with this bacterium.

Thanks, neighbor! Stock market experience around significant European tax cuts

PurposeThis paper analyzes the effect of corporate tax cuts on the competitiveness of the tax-cutting countries and neighbor countries.Design/methodology/approachThis study utilizes four significant corporate tax reforms among the OECD countries in Europe that offer a one-time tax cut of 6% or more. The short-term event study approach examines the stock index reactions for both the tax-cutting countries and the other countries. Multivariate fixed-effect regressions are employed to study the cross-sectional variations in the non-tax-cut countries.FindingsThis paper finds positive excess returns for Slovakia and Germany around the tax-cut passage. Multivariate analysis of stock market reactions of the non-tax-cutting countries reveals some evidence supporting both the positive spillover effect and the negative competitive loss effect. More advanced countries are more likely to experience higher abnormal returns, while higher tax countries are more likely to suffer lower abnormal returns. Other factors identified that might have influenced the effect of a foreign tax cut include the existing trade flows with the tax-cutting countries, whether the country has a common currency and the export orientation of the economy.Research limitations/implicationsThe findings are subject to sample-size issues. The lack of results for the other two countries is due to complicating events, as suggested by the further investigation of concurrent news events around the event days.Practical implicationsThe simultaneous analysis of the reform countries and the other countries in the region suggests that policymakers need to consider the relative positioning of their country vs the other countries in terms of economic development and current tax burdens when determining the optimal policy for their country or to respond to the tax policy changes in the other countries.Originality/valueThis study offers empirical evidence regarding the effect of corporate tax changes on competitiveness through the lens of stock markets' reactions, which depend on the net results of the spillover gain vs the competitive loss.

C-Fos is upregulated in the genital tubercle of DEHP-induced hypospadiac rats and the prepuce of patients with hypospadias

ABSTRACT This study aimed to investigate the expression of Fos proto-oncogene, AP-1 transcription factor subunit (c-Fos) in the genital tubercle (GT) of rats with di(2-ethylhexyl) phthalate (DEHP)-induced hypospadias and in the prepuce of patients with hypospadias compared with unaffected controls. Pregnant rats were given 750 mg/kg/day DEHP orally from gestational days 12–19. Western blotting showed that c-Fos expression was increased in DEHP-induced hypospadiac male offspring. In addition, 30 prepuce tissue specimens obtained during hypospadias repair surgery were divided into 2 groups: the mild hypospadias group (n = 15) and the severe hypospadias group (n = 15). Fifteen normal prepuce tissue specimens were harvested during elective circumcision as normal controls. Real-time quantitative polymerase chain reaction, western blotting and immunohistochemistry analyses were used to assess c-Fos expression. c-Fos protein levels were higher in the GT of DEHP-induced rats than in that of control rats. c-Fos mRNA and protein levels were also higher in the hypospadias groups than in the control group (p < 0.05, p < 0.001), and c-Fos protein levels were significantly higher in the severe hypospadias group than in the mild hypospadias group (p < 0.01). The expression of c-Fos was increased in both the GT of DEHP-induced hypospadiac rats and the prepuce of hypospadias patients. Thus, c-Fos overexpression might contribute to hypospadias. Abbreviations: DEHP: di(2-ethylhexyl) phthalate; c-Fos: Fos proto-oncogene, AP-1 transcription factor subunit; Mafb: the masculinization-regulatory gene v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B; GT: genital tubercle; ED: embryonic day; AGD: anogenital distance; AGI: anogenital distance index; ED: embryonic day.

Fluorite enhanced magnesium recovery from serpentine tailings: Kinetics and reaction mechanisms

Assisted reagents are commonly introduced into acid leaching systems to improve metal recovery. In this work, fluorite was employed to accelerate magnesium (Mg) dissolution from serpentine tailings, and the dissolution mechanism was investigated by leaching experiments, kinetics equations and reaction analyses. The effects of fluorite powder dosage, particle size, reaction temperature, sulfuric acid concentration, and agitation speed on the efficiency of magnesium leaching were comprehensively studied. The results showed that the Mg leaching efficiency increased 36.42% after adding fluorite, reaching 93.69%. A shrinking-core model could be applied to adequately describe the leaching kinetics, and the Mg dissolution was controlled by the mixing rate with a calculated apparent activation energy of 38.698 kJ·mol−1. In addition, a kinetics equation describing the leaching process was established as follows: 1/3ln(1-x)-1 + (1-x)-1/3 = 70.372P-1.071C3.17S0.497e-38,698.968/RTt. Introducing the fluorite powder markedly decreased the ∆GΘ of serpentine disintegration process; these phenomena was attributed to the complexation of F− (fluorite) with Si4+ (serpentine). Therefore, this paper proposed an effective and inexpensive reagent for application in acid leaching processes.

Approximation error model (AEM) approach with hybrid methods in the forward-inverse analysis of the transesterification reaction in 3D-microreactors

This work advances the approximation error model approach for the inverse analysis of the biodiesel synthesis using soybean oil and methanol in 3D-microreactors. Two hybrid numerical-analytical approaches of reduced computational cost are considered to offer an approximate forward problem solution for a three-dimensional nonlinear coupled diffusive-convective-reactive model. First, the Generalized Integral Transform Technique (GITT) is applied using approximate non-converged solutions of the 3D model, by adopting low truncation orders in the eigenfunction expansions. Second, the Coupled Integral Equations Approach (CIEA) provides a reduced mathematical model for the average concentrations, which leads to inherently approximate solutions. The AEM approach through the Bayesian framework is illustrated in the simultaneous estimation of kinetic and diffusion coefficients of the transesterification reaction. For this purpose, the fully converged GITT results with higher truncation orders for the 3D partial differential model are employed as reference results to define the approximations errors. The results highlight that either the non-converged solutions via GITT or the reduced model solution obtained via CIEA, when taking into account the model error, are robust and cost-effective alternatives for the inverse analysis of nonlinear convection–diffusion-reaction problems.

Rho-kinase inhibitor hydroxyfasudil protects against HIV-1 Tat-induced dysfunction of tight junction and neprilysin/A\u03b2 transfer receptor expression in mouse brain microvessels

HIV-1 transactivator protein (Tat) induces tight junction (TJ) dysfunction and amyloid-beta (Aβ) clearance dysfunction, contributing to the development and progression of HIV-1-associated neurocognitive disorder (HAND). The Rho/ROCK signaling pathway has protective effects on neurodegenerative disease. However, the underlying mechanisms of whether Rho/ROCK protects against HIV-1 Tat-caused dysfunction of TJ and neprilysin (NEP)/Aβ transfer receptor expression have not been elucidated. C57BL/6 mice were administered sterile saline (i.p., 100 μL) or Rho-kinase inhibitor hydroxyfasudil (HF) (i.p., 10 mg/kg) or HIV-1 Tat (i.v., 100 μg/kg) or HF 30 min before being exposed to HIV-1 Tat once a day for seven consecutive days. Evans Blue (EB) leakage was detected via spectrophotometer and brain slides in mouse brains. The protein and mRNA levels of zonula occludens-1 (ZO-1), occludin, NEP, receptor for advanced glycation end products (RAGE), and low-density lipoprotein receptor-related protein 1 (LRP1) in mouse brain microvessels were, respectively, analyzed by Western blotting and quantitative real-time polymerase chain reaction (qRT-PCR) analyses. Exposure of the mice to HIV-1 Tat increased the amount of EB leakage, EB fluorescence intensity, blood–brain barrier (BBB) permeability, as well as the RAGE protein and mRNA levels, and decreased the protein and mRNA levels of ZO-1, occludin, NEP, and LRP1 in mouse brain microvessels. However, these effects were weakened by Rho-kinase inhibitor HF. Taken together, these results provide information that the Rho/ROCK signaling pathway is involved in HIV-1 Tat-induced dysfunction of TJ and NEP/Aβ transfer receptor expression in the C57BL/6 mouse brain. These findings shed some light on potentiality of inhibiting Rho/Rock signaling pathway in handling HAND.

Differential Expression Profiles and Functional Analysis of Long Non-coding RNAs in Children With Dilated Cardiomyopathy.

Aim: To evaluate the expression profile of long non-coding RNAs (lncRNAs) in different left ventricular function of dilated cardiomyopathy (DCM) in children and explore their possible functions.Methods: The lncRNA microarray experiment was used to determine the differential expression profile of lncRNA in three children with DCM and three healthy volunteers. The functional analysis and the construction of the lncRNA-mRNA interaction network were carried out to study the biological functions. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to verify the microarray data.Results: There were 369 up-regulated lncRNAs identified in the DCM patients (fold change >2, P < 0.05), and 505 down-regulated lncRNAs. Based on target gene prediction and co-expression network construction, 9 differentially expressed lncRNAs were selected for the PCR to verify the accuracy of the microarray data, of which 5 were up-regulated and 4 were down-regulated, and finally proved that 7 of them were consistent with the trend of microarray data results. Four of these lncRNAs had significant differences between the patients with poor cardiac function and patients with improved left ventricle function.Conclusion: LncRNAs may play an important role in pediatric DCM and may provide a new perspective for the pathogenesis, diagnosis, and treatment of this disease.

CD10 marks non-canonical PPAR\u03b3-independent adipocyte maturation and browning potential of adipose-derived stem cells

BackgroundEffective stem cell therapy is dependent on the stem cell quality that is determined by their differentiation potential, impairment of which leads to poor engraftment and survival into the target cells. However, limitations in our understanding and the lack of reliable markers that can predict their maturation efficacies have hindered the development of stem cells as an effective therapeutic strategy. Our previous study identified CD10, a pro-adipogenic, depot-specific prospective cell surface marker of human adipose-derived stem cells (ASCs). Here, we aim to determine if CD10 can be used as a prospective marker to predict mature adipocyte quality and play a direct role in adipocyte maturation.MethodsWe first generated 14 primary human subject-derived ASCs and stable immortalized CD10 knockdown and overexpression lines for 4 subjects by the lentiviral transduction system. To evaluate the role of CD10 in adipogenesis, the adipogenic potential of the human subject samples were scored against their respective CD10 transcript levels. Assessment of UCP1 expression levels was performed to correlate CD10 levels to the browning potential of mature ASCs. Quantitative polymerase chain reaction (qPCR) and Western blot analysis were performed to determine CD10-dependent regulation of various targets. Seahorse analysis of oxidative metabolism and lipolysis assay were studied. Lastly, as a proof-of-concept study, we used CD10 as a prospective marker for screening nuclear receptor ligands library.ResultsWe identified intrinsic CD10 levels as a positive determinant of adipocyte maturation as well as browning potential of ASCs. Interestingly, CD10 regulates ASC’s adipogenic maturation non-canonically by modulating endogenous lipolysis without affecting the classical peroxisome proliferator-activated receptor gamma (PPARγ)-dependent adipogenic pathways. Furthermore, our CD10-mediated screening analysis identified dexamethasone and retinoic acid as stimulator and inhibitor of adipogenesis, respectively, indicating CD10 as a useful biomarker for pro-adipogenic drug screening.ConclusionOverall, we establish CD10 as a functionally relevant ASC biomarker, which may be a prerequisite to identify high-quality cell populations for improving metabolic diseases.

Analysis of reaction\u2013diffusion systems where a parameter influences both the reaction terms as well as the boundary

We study positive solutions to steady-state reaction–diffusion models of the form {−Δu=λf(v);Ω,−Δv=λg(u);Ω,∂u∂η+λu=0;∂Ω,∂v∂η+λv=0;∂Ω,\\documentclass[12pt]{minimal}\t\t\t\t\\usepackage{amsmath}\t\t\t\t\\usepackage{wasysym}\t\t\t\t\\usepackage{amsfonts}\t\t\t\t\\usepackage{amssymb}\t\t\t\t\\usepackage{amsbsy}\t\t\t\t\\usepackage{mathrsfs}\t\t\t\t\\usepackage{upgreek}\t\t\t\t\\setlength{\\oddsidemargin}{-69pt}\t\t\t\t\\begin{document}$$ \\textstyle\\begin{cases} -\\Delta u=\\lambda f(v);\\quad\\Omega, \\\\ -\\Delta v=\\lambda g(u);\\quad\\Omega, \\\\ \\frac{\\partial u}{\\partial \\eta }+\\sqrt{\\lambda } u=0;\\quad \\partial \\Omega, \\\\ \\frac{\\partial v}{\\partial \\eta }+\\sqrt{\\lambda }v=0; \\quad\\partial \\Omega, \\end{cases} $$\\end{document} where lambda >0 is a positive parameter, Ω is a bounded domain in mathbb{R}^{N}(N > 1) with smooth boundary ∂Ω, or Omega =(0,1), frac{partial z}{partial eta } is the outward normal derivative of z. We assume that f and g are continuous increasing functions such that f(0) = 0 = g(0) and lim_{s rightarrow infty } frac{f(Mg(s))}{s} = 0 for all M>0. In particular, we extend the results for the single equation case discussed in (Fonseka et al. in J. Math. Anal. Appl. 476(2):480-494, 2019) to the above system.

Analysis of Surface Reaction for Group III-V Compound Semiconductors in Functional Water

Group III–V compound semiconductors are attracting attention as new channel materials that have higher carrier mobility than Si. However, defects easily occur at the interface between the semiconductor and insulator film, which degrades performance. In an earlier study, we demonstrated that the interfacial properties of InP are degraded by the growth of In2O3 and that In2O3 grows better in water than in air. Therefore, it is necessary to suppress the growth of In2O3 to improve the interfacial properties of InP. In this work, we focused on functional water, which can be controlled by adjusting the water conditions, and investigated the growth behavior of In2O3 in functional water. As a result, we found that the growth is suppressed in the low-pH range and in hydrogen water. It is important that H+ ions reduce OH− ions, which contributes to the reaction with InP.