Abstract
Aim: To evaluate the expression profile of long non-coding RNAs (lncRNAs) in different left ventricular function of dilated cardiomyopathy (DCM) in children and explore their possible functions.Methods: The lncRNA microarray experiment was used to determine the differential expression profile of lncRNA in three children with DCM and three healthy volunteers. The functional analysis and the construction of the lncRNA-mRNA interaction network were carried out to study the biological functions. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis was used to verify the microarray data.Results: There were 369 up-regulated lncRNAs identified in the DCM patients (fold change >2, P < 0.05), and 505 down-regulated lncRNAs. Based on target gene prediction and co-expression network construction, 9 differentially expressed lncRNAs were selected for the PCR to verify the accuracy of the microarray data, of which 5 were up-regulated and 4 were down-regulated, and finally proved that 7 of them were consistent with the trend of microarray data results. Four of these lncRNAs had significant differences between the patients with poor cardiac function and patients with improved left ventricle function.Conclusion: LncRNAs may play an important role in pediatric DCM and may provide a new perspective for the pathogenesis, diagnosis, and treatment of this disease.
Highlights
Dilated cardiomyopathy (DCM) is the main cause of progressive refractory heart failure which necessitates heart failure and can lead to death [1]
The annual incidence of DCM in children accounts for approximately 50% of pediatric cardiomyopathies (0.57/100,000) and is the most common type of pediatric cardiomyopathy [3, 4]
The Microarray Analysis detected a total of 75,589 Long non-coding RNA (lncRNA) in 3 DCM samples and 3 control samples
Summary
Dilated cardiomyopathy (DCM) is the main cause of progressive refractory heart failure which necessitates heart failure and can lead to death [1]. DCM is defined by the presence of a dilated left ventricle with systolic dysfunction in the absence of a hemodynamic cause which can lead to dilation and dysfunction [2]. The annual incidence of DCM in children accounts for approximately 50% of pediatric cardiomyopathies (0.57/100,000) and is the most common type of pediatric cardiomyopathy [3, 4]. The mortality rate of children with DCM is high, and most children with this condition die from heart failure [5]. High-risk factors of children with DCM include decreased left ventricular function, left ventricular dilation, and left ventricular posterior wallthinning [6].
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