The relationship between epilithic biomass and oxygen depletion was studied above and below wastewater discharges to the Manawatu River in an attempt to guide river management by defining the maximum respiration rates and the corresponding epilithic biomass consistent with maintenance of the legal minimum DO concentration of 5 g m −3. A computer model, which simulated the river DO under lowflow conditions, predicted that the maximum allowable respiration rate giving a dawn DO concentration of 5 g m −3 ranged from 20 g O 2 m −2 d −1 at 21°C to 24.5 g O 2 m −2 d −1 at 12°C. A multiple regression model developed from chamber measurements made in situ showed that the total biomass density and water temperature accounted for 73% of the variation in the respiration rate observed. When adapted to allow for respiration occurring in the water column, the model gave reasonably accurate predictions of respiration when checked against the whole river respiration rate measurements. Biomass densities, at which DO levels reach 5 gm −3 predicted using these models, ranged from 34 g ash free dry wt m −2 at 21°C to 143 g ash free dry wt m −2 at 12°C. Analyses of historical hydrological data and the results of epilithon growth measurements under a variety of water quality conditions in the Manawatu River indicate that the present management strategy of limiting instream BOD 5 to below 5 g m −3 but not limiting nutrient (N and P) levels will not prevent the occurrence in summer of the predicted nuisance biomasses and hence unacceptable deoxygenation.