Analysis Of Bioactive Components Research Articles

Analysis of bioactive components and synergistic action mechanism of ShuGan-QieZhi Capsule for treating non-alcoholic fatty liver disease

BackgroundShuGan-QieZhi capsule (SGQZC) is a traditional Chinese preparation used to treat hyperlipidemia and obesity, even non-alcoholic fatty liver disease (NAFLD). However, its therapeutic effects, main bioactive ingredients, as well as potential mechanisms for NAFLD are still unclear. PurposeTo investigate the pharmacological effect, main active ingredients, and mechanisms of SGQZC against high-fat diet (HFD)-induced NAFLD in mice. MethodsNAFLD models were established by feeding C57BL/6 J mice an HFD for 24 weeks. From the 12th week, HFD-fed mice received daily gavage of either SGQZC or silibinin for 12 weeks. Hepatic hypertrophy parameters, along with hepatic and systemic lipid metabolism changes in NAFLD mice, were assessed. Oil red O and histopathological staining techniques determined lipid accumulation and liver injury severity. qRT-PCR analysis measured the expression of genes tied to liver lipid metabolism and inflammation. HPLC-MS/MS identified the primary components of SGQZC in the serum. Human normal hepatocytes (LO2) and hepatic stellate cells (LX-2) were used to screen SGQZC's bioactive ingredients. Network pharmacological analysis, transcriptomics, and western blotting delved into SGQZC's synergistic mechanisms against NAFLD. ResultsSGQZC ameliorated abnormal lipid metabolism and liver hypertrophy in mice with HFD-induced NAFLD, consequently reducing hepatic lipid accumulation, inflammatory cell infiltration, and liver impairment. Eight crucial components of SGQZC were detected in serum using HPLC-MS/MS and were found to effectively attenuate lipid accumulation and inflammation in liver cells. Further investigation indicated that SGQZC modulates MAPK pathway and AKT/NF-κB pathway, subsequently improving lipid metabolism and inflammation. ConclusionSGQZC alleviates NAFLD by synergistically modulating the MAPK-mediated lipid metabolism and inhibiting AKT/NF-κB pathways-mediated inflammation. Our findings reveal the enormous potential of SGQZC for the treatment of NAFLD, providing a possible new clinical therapeutic strategy.

Enzyme activity- and chemometrics-assisted comprehensive two-dimensional liquid chromatography coupled with ion mobility quadrupole time-of-flight mass spectrometry for the analysis of honeysuckle

A unique and effective comprehensive two-dimensional liquid chromatography system was established and applied for the analysis of bioactive components in honeysuckle. Under the optimal conditions, Eclipse Plus C18 (2.1 × 100 mm, 3.5 μm, Agilent) and SB-C18 (4.6 × 50 mm, 1.8 μm, Agilent) columns were chosen for the first dimension (1D) and the second dimension (2D) separation. The optimal flow rates of 1D and 2D were 0.12 mL/min and 2.0 mL/min, respectively. Additionally, the proportion of organic solution was optimized to enhance orthogonality and integrated shift, and full gradient elution mode was adopted to improve chromatographic resolution. Furthermore, a total of 57 compounds were identified by molecular weight, retention time and collision cross-section value obtained from ion mobility mass spectrometry. Based on the data obtained from the principal component analysis, partial least squares discriminant analysis, and hierarchical cluster analysis, the categories of honeysuckle in different regions were significantly different. Moreover, the half maximal inhibitory concentration values of most samples were between 0.37 and 1.55 mg/mL, and most samples were potent α-glucosidase inhibitors, which is better for the evaluation of the quality of drugs from two aspects of substance content and activity.

Comparative Analysis of Bioactive Components of Enzymatically and Fermentatively Produced Fish Sauce from Sardine

Fish sauce, a flavored condiment produced by traditional fermentation of low-value fish. The time of fermentation and biochemical safety of sauce are the major concern in traditional fermentation. The enzymatic and fermentative methods were employed to produce Sardine (Sardinella longiceps) sauce under optimized conditions using response surface method. Salt (20%, w/w) and papain (3%, w/w) were used for enzymatic production; while, salt (25%, w/w), sugar (7.5%, w/w) and lactic culture (10%, w/v; Pediococcus pentosaceus FSBP4-40) were employed in fermentative production. Significant change in bioactive components such as pH, non-enzymatic browning, non-protein nitrogen, degree of hydrolysis, titratable acidity, total soluble nitrogen, and total volatile base nitrogen was observed in treated samples compared to the control. Total antioxidant activity (as ascorbic acid, μg/ml), percent DPPH, ABTS, and superoxide anion scavenging activities of fermented sauce were higher at 3976±90, 53.11±0.5, 99.6±0.3, 67.7±0.5 respectively; as compared to enzyme-treated samples which showed 952±70, 31.46±0.5, 76.5±1.0, 61.8±1.4 for the respective parameters, after 180 days storage. Reducing potential was found significantly higher (P<0.05) in treated sauces compared to the control and fatty acid profile showed different changes. The study emphasizes the role of accelerating fermentation, either enzymatically or fermentatively, to produce biochemically and biofunctional superior Sardine sauce.

Karakteristik Kandungan Kimia dan Komponen Bioaktif Rumput Laut Hijau Halimeda sp. Dari Kepulauan Seribu

Halimeda sp is a green seaweed belonging to the order Bryopsidales, class Chlorophyta. Production data from the genus Halimeda sp in Indonesia yet has not been reported by the Ministry of Fisheries and Marine Affairs because it belonged to the unknown type of potential. The purpose of this study was to determine the chemical and bioactive components of Halimeda green seaweed. The chemical components of seaweed Halimeda sp included of moisture content, ash, protein, fat, and carbohydrates (by different). Analysis of bioactive components was carried out qualitatively and quantitatively by counted total phenol. The results showed that the proximate analysis of Halimeda sp contained 3.15% moisture content, 87.61% ash content, 0.2% protein content, 1% fat content, and 11.19% carbohydrate (by different) content. Qualitatively Halimeda sp positively contains steroid and alkaloid compounds and was negative for flavonoids, phenols, tannins, saponins, and triterpenoids. The quantitative analysis of the total phenol content of Halimeda sp. extracted with 99.9% ethanol solvent was 273.88 mg GAE/g.

Garlic-derived exosome-like nanovesicles alleviate dextran sulphate sodium-induced mouse colitis via the TLR4/MyD88/NF-κB pathway and gut microbiota modulation.

Plant-derived exosome-like nanovesicles play an important role in transferring their biological cargos to recipient cells. The effect of garlic-derived exosome-like nanovesicles (GENs) against inflammatory bowel disease (IBD) remains unknown. This study aimed to investigate the effect of GENs on dextran sulphate sodium (DSS)-induced colitis in mice. A comprehensive analysis of bioactive components in GENs was performed. Data showed that GENs contained 26 lipids, 61 proteins and 127 known microRNAs (miRNAs). Han-miR3630-5p in GENs could bind to the 3' untranslated region of toll-like receptor 4 (TLR4), which led to the inhibition of TLR4 expression. Besides, GENs significantly up-regulated the expression of barrier-related proteins and inhibited the overproduction of pro-inflammatory cytokines in LPS-induced Caco-2 cells. As a result, pretreatment with GENs at 100 mg kg-1 efficiently ameliorated the inflammatory bowel behavior, intestinal histological pathological damage, and tight junction protein dysfunction induced by DSS in the colon tissue. Intake of GENs significantly down-regulated the expressions of TLR4, myeloid differentiation primary response gene 88 (MyD88), and nuclear factor kappa-B (NF-κB), which suppressed the downstream cascades and led to less secretion of pro-inflammatory cytokines induced by DSS. Furthermore, pretreatment with GENs altered the gut microbiota profile of colitis mice by recovering the relative abundance of Lachnospiraceae and reducing the relative abundance of Helicobacter. Totally, GENs had potential to protect the colon against DSS-induced damage through inhibiting the TLR4/MyD88/NF-κB signaling pathway and regulating gut microbiota. This study clarified the role of miRNAs of GENs in anti-colitis and proved that GENs had a potential application for IBD prevention.

Analysis of Bioactive Components and Antioxidant Capacities of Some Tropical Fruits Adapted to the Mediterranean Region

Türkiye sahip olduğu ekolojik özellikleri sebebiyle birçok bitki türüne ev sahipliği yapmaktadır. Bununla birlikte Türkiye’nin farklı iklimsel özelliklere sahip olan farklı bölgelerinde yeni meyve türlerinin adaptasyon çalışmaları her geçen gün artmaktadır. Bu çalışmanın amacı Akdeniz Bölgesine adapte edilen Mango (Mangifera indica L.), beyaz (Hylocereus undatus Britton & Rose) ve kırmızı pitaya (Hylocereus lemairei (Hook.) Britton & Rose), guava (Psidium guajava L.) ve bölgede yaygın olarak yetişen mart inciri (Opuntia ficus-indica (L.) Mill.) meyvelerinin etinde toplam fenolik madde, toplam flavanoid madde, toplam askorbik asit ve antioksidan kapasitenin (ABTS, DPPH) belirlenmesidir. En yüksek toplam fenolik madde ve toplam flavanoid madde içeriği sırasıyla guava (80.1 mg 100g-1) ve mart inciri (4.68 mg 100g-1) meyvelerinde; en düşük mango (45.4 mg 100g-1) ve beyaz pitaya (0.95 mg 100g-1) meyvelerinde tespit edilmiştir. Kırmızı pitaya meyve etinde renk maddelerinin de içerisinde yer aldığı toplam flavanoid madde içeriği beyaz pitaya meyvelerinden daha yüksek bulunmuştur. Toplam askorbik asit içeriği en yüksek olan meyve mart inciri meyvesidir (5.38 mg 100g-1). ABTS ve DPPH radikal süpürücü aktiviteler ise guava meyvesinde en yüksek bulunurken, mart inciri meyvesinde en düşük olarak belirlenmiştir.

Phytonutrient and antinutrient components profiling of Berberis baluchistanica Ahrendt bark and leaves

ObjectivesPlants are the most prevalent primary natural source of active medications. Berberis baluchistanica Ahrendt is known for the treatment of various ailments. Bioactive components, nutritional and antioxidant capacity of Berberis baluchistanica bark and leaves ethanolic extracts were evaluated in this study. MethodsTotal phenolics, flavonoids, antioxidant, nutritional and anti-nutritional contents were analyzed. Analysis of bioactive components identified the presence of tannins, cardiac glycosides, flavonoids, coumarin, alkaloids, phenolics, saponins, steroids, anthraquinones and terpenoids. The capability of donating hydrogen was analyzed by their 50% inhibition concentration (IC50). ResultsThe bark possessed lower IC50 = 0.678 mg/mL and higher inhibition percentage of DPPH radical, compared to leaves IC50 = 0.785 mg/mL. The Ferric reducing antioxidant power of bark was relatively higher IC50 = 0.871 mg/mL than leaves IC50 = 0.996 mg/mL. The phenolic content of bark was 37.52 ± 1.56 mg GAE/g and that of leaves 28.32 ± 0.66 mg GAE/g, the total flavonoid contents in bark and leaves were 8.68 ± 0.93 and 11.81 ± 1.49 mg QE/g. Total proteins of the bark and leaves were 7.69 ± 0.65 mg BSAE/g and 3.63 ± 0.54 mg BSAE/g and carbohydrate contents of the bark and leaves were 4.46 ± 0.55 mg GE/g and 8.38 ± 0.71 mg GE/g respectively. The oxalate contents of bark were 0.12 ± 0.02 mg/g and leaves were 0.14 ± 0.19 mg/g and the phytate % composition of bark was 0.17 ± 0.24 % and leaves were 0.25 ± 0.08 % respectively. ConclusionsThe determination of these compounds attaining a range of medicinal properties helps in maintaining the traditional use of bark and leaves extracts of Berberis baluchistanica in various biomedical fields.

Network Pharmacology Analysis of Bioactive Components and Mechanisms of Action ofQi Wei WanFormula for Treating Non-Small Cell Lung Carcinoma

Astragali Radix (AR) and Schisandrae chinensis Fructus (SCF) have been used individually and in traditional Chinese medicine (TCM) formulas for treating non-small cell lung carcinoma (NSCLC). Qi Wei Wan (QWW), a 2-herb TCM formula composed of AR and SCF, is used to treat blood deficiency, fatigue, and metabolic abnormalities. We speculate that QWW may be more effective in treating NSCLC than AR or SCF alone. We identified 28 bioactive compounds in QWW and 322 targets of these compounds from databases. Network pharmacology analysis was used to identify 248 putative NSCLC-related gene targets of the bioactive compounds in QWW. Common target genes were analyzed to build protein–protein interaction networks. Implicated biological functions and pathways (p53, PI3K-Akt, etc) were identified by Kyoto Encyclopedia of Genes and Genomes and Gene Ontology analyses. Molecular docking of core target proteins with the key active compounds was also performed. This study identified the potential gene targets and mechanisms involved in the anti-NSCLC effects of QWW.

Two-dimensional chromatography in screening of bioactive components from natural products.

Screening and analysis of bioactive components from natural products is a fundamental part of new drug development and innovation. Two-dimensional (2D) chromatography has been demonstrated to be an effective method for screening and preparation of specific bioactive components from complex natural products. To collect details of application of 2D chromatography in screening of natural product bioactive components and to outline the research progress of different separation mechanisms and strategies. Three screening strategies based on 2D chromatography are reviewed, including traditional separation-based screening, bioactivity-guided screening and affinity chromatography-based screening. Meanwhile, in order to cover these aspects, selections of different separation mechanisms and modes are also presented. Compared with traditional one-dimensional (1D) chromatography, 2D chromatography has unique advantages in terms of peak capacity and resolution, and it is more effective for screening and identifying bioactive components of complex natural products. Screening of natural bioactive components using 2D chromatography helps separation and analysis of complex samples with greater targeting and relevance, which is very important for development of innovative drug leads.

Food Waste Management Employing UV-Induced Black Soldier Flies: Metabolomic Analysis of Bioactive Components, Antioxidant Properties, and Antibacterial Potential.

Food waste, as a major part of municipal solid waste, has been increasingly generated worldwide. Efficient and feasible utilization of this waste material for biomanufacturing is crucial to improving economic and environmental sustainability. In the present study, black soldier flies (BSF) larvae were used as carriers to treat and upcycle food waste. Larvae of the BSF were incubated with UV light for 10, 20, and 30 min at a wavelength of 257.3 nm and an intensity of 8 W. The food waste utilization efficiency, antioxidant assays, antibacterial activity, and bioactive metabolites without and with UV treatment were determined and compared. Results showed that the BSF larvae feed utilization rate was around 75.6%, 77.7%, and 71.2% after UV treatment for 10, 20, and 30 min respectively, contrasting with the non-UV induced group (73.7%). In addition, it was perceived that the UV exposure enhanced antioxidant and antimicrobial properties of BSF extracts, and the maximum values were observed after 20 min UV induction time. Moreover, UV-induced BSF extracts showed an improved metabolic profile than the control group, with a change in the amino acids, peptides, organic acids, lipids, organic oxides, and other derivatives. This change in metabolomics profile boosted environmental signaling, degradation of starch, amino acids, sugars, and peptide metabolism. It was concluded that the bioconversion of food wastes using UV-induced BSF larvae can enhance the generation of a variety of functional proteins and bioactive compounds with potent antioxidant and antimicrobial activity. However, more studies are required to exploit the efficiency of UV treatment in improving BSF’s potential for upcycling of food wastes.

Insilico and Pharmacological Property Analysis of Bioactive Components from <i>Prunus avium</i> against Diabetics

Diabetes is a common metabolic disorder, which effects people across all cultures globally. Lifelong distress is the cause of this disease which has no cure as of now. Various medications available in the market are too expensive and not easily affordable by all. Rural people rely on plant based Ayurvedic medications to heal diabetes as these contain anti-diabetic compounds. These phytoconstituents/anthocyanin derivatives work with several mechanisms that involve phytoconstituent interactions and target molecules in diabetic metabolism. Molecular docking analysis aids in finding out the interaction between receptors and ligands to identify the finest interaction which suits the target. In this case, the study proposed examining the bonding interactions of anti-diabetic compounds/anthocyanin derivatives derived from medicinal plants (Pelargonidin, Cyanidin, Delphinidin, Peonidin, Petunidin, Quercetin and pancreatic alpha-amylase (4X9Y)) with the help of the computational tool. ADME/T test helps decide different pharmacological and physicochemical analysis of lead atoms, degree of adsorption inside the cell, digestion rate, solvency, blood cerebrum boundary penetrability, cancer-causing nature and so on, which are the significant essentials prior to advertising a medication. Peonidin and Quercetin was proposing the best interactions. Nonetheless, to discover a better cure for diabetes, further in-vitro/ in vivo studies have to be carried out.

Phytochemical Analysis of Bioactive Components of Medicinal Plants

Phytochemical Analysis of Bioactive Components of Medicinal Plants

Phytochemistry and gas chromatography-mass spectrometry (GC-MS) analysis of bioactive components of methanolic root-bark extract of Boswellia dalzielii (burseraceae)

This study was conducted to identify phytochemical constituents present in the methanolic root-bark extract of B. dalzielii using GC-MS analysis and conventional phytochemical evaluation. The studies revealed the presence of alkaloids, flavonoids, saponins, cardiac glycosides, terpenoids and carbohydrates. A total of forty three compounds composed of eighteen major components were identified thus; oxetane-2,3,4-trimethyl (3.45%), Bicyclo[4.1.0] heptan-3-one-7,7-dimethyl-4-methylene(IR) [2.69%], 2-methyl-2-(−2-oxopropyl) furan (3.20%), Cyclohexane, 2-(dimethylhydrazono)-3-[4-hexenyl]-1-acinitro,(E,) [2.63%], 3,8-Dihydroxy-3,4-dihydronaphthalen-1(2H)one (4.43%), 2-Tetradocanol (4.89%), 3-methyl-2-(2-oxopropyl)furan (3.46%), (2H)-Naphthalenenone, 6-(1,1-dimethyl ethyl) octahydro-2,8a-dimethyl (3.80%), Pentadecanoic acid (6.18%), undecanoic acid (3.68%), Phthalic acid (4.14%), 1-Allyl-cyclohexane-1,2-diol (3.95%), 1,5,9-cyclotetradecatriene, 1,5,9-trimethyl-12-(1-methyl ethyl) [4.58%], 9,12-octadecadienoic acid (Z,Z)-methyl ester (4.75%). 9-octadecenoic acid, methyl ester[E] (4.36%), Tetradecanoic acid-methyl ester(S) [3.90%], (2S,4R)-p-mentha-[1(7),8]-diene-2-hydroperoxide (2.62%) and 1-Naphthalenepropanol, a-ethyldecahydro-8a-(hydroxymethyl)-a, 5-dimethyl-2-methylene (2.93%). Most of the identified compounds have been reported to possess various biological activities such as antimicrobial, anti-inflammatory, antimalarial, antidiabetics, anti-asthmatic, anti-histamine, antitubercular, anti-diarrhoeal among others. The studies thus concluded that B. dalzielii root-bark possesses potent bioactive compounds and is recommended as a plant of phytopharmaceutical importance. The presence of these bioactive compounds justifies the use of this plant in ethnomedicine.

A strategy of screening and binding analysis of bioactive components from traditional Chinese medicine based on surface plasmon resonance biosensor

Elucidating the active components of traditional Chinese medicine (TCM) is essential for understanding the mechanisms of TCM and promote its rational use as well as TCM-derived drug development. Recent studies have shown that surface plasmon resonance (SPR) technology is promising in this field. In the present study, we propose an SPR-based integrated strategy to screen and analyze the major active components of TCM. We used Radix Paeoniae Alba (RPA) as an example to identify the compounds that can account for its anti-inflammatory mechanism via tumor necrosis factor receptor type 1 (TNF-R1). First, RPA extraction was analyzed using an SPR-based screening system, and the potential active ingredients were collected, enriched, and identified as paeoniflorin and paeonol. Next, the affinity constants of paeoniflorin and paeonol were determined as 4.9 and 11.8 μM, respectively. Then, SPR-based competition assays and molecular docking were performed to show that the two compounds could compete with tumor necrosis factor-α (TNF-α) while binding to the subdomain 1 site of TNF-R1. Finally, in biological assays, the two compounds suppressed cytotoxicity and apoptosis induced by TNF-α in the L929 cell line. These findings prove that SPR technology is a useful tool for determining the active ingredients of TCM at the molecular level and can be used in various aspects of drug development. The SPR-based integrated strategy is reliable and feasible in TCM studies and will shed light on the elucidation of the pharmacological mechanism of TCM and facilitate its modernization.

Visualizing the spatial distribution of metabolites in Clausena lansium (Lour.) skeels using matrix-assisted laser desorption/ionization mass spectrometry imaging

Clausena lansium (Lour.) Skeels (Rutaceae) is a natural bioactive plant. Its roots, stems, leaves, and seeds are widely used in Chinese traditional and folk medicine. Although the characterization and functional analysis of bioactive components in Clausena lansium (Lour.) Skeels has been widely reported, the spatial distribution of these compounds within the main plant tissues remains undefined. Here, we adopted matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) to reveal the spatial distribution of active alkaloids, coumarins, sugars and organic acids in C. lansium. Using a combined wet and dry matrix covering method to enhance sensitivity, we detected alkaloids throughout the fruit including 3-methylcarbazole and murrastinine which were especially rich in the kernel tissues but were restricted to the stem xylem and medulla and in the leaf epidermal region. Interestingly, murrayanine and heptaphylline were mainly found in pulp tissues with very low content in the stems and leaves while girinimbine was only distributed within the outer kernel skin. Coumarins were mainly distributed in the fruit pericarp and leaf vein tissues but with no clear spatial specificity in stems. Lastly, hexoses were mainly evident in the fruit pulp, although sucrose was also found in the pericarp, pulp, and pulp fibers with citric acid being distributed throughout the fruit. The accurate spatial and chemical information obtained provides new insights into the specific accumulation of metabolites in individual tissues.

GC-MS Analysis of Bioactive Components in the Methanolic Extract of Graviola (Gishta) Seeds and Their Pharmacological Activities

GC-MS Analysis of Bioactive Components in the Methanolic Extract of Graviola (Gishta) Seeds and Their Pharmacological Activities

Analysis of Bioactive Components in the Fruit, Roots, and Leaves of Alpinia oxyphylla by UPLC-MS/MS.

Alpinia oxyphylla (A. oxyphylla) fruit has long been used in traditional Chinese medicine. In our study, the bioactive components of its roots, fruit, and leaves were investigated, and their potential medical value was predicted. The root, fruit, and leaf samples were analyzed using a UPLC-MS/MS system. The mass spectrometry outcomes were annotated by MULTIAQUANT. The “compound-disease targets” were used to construct a pharmacology network. A total of 293, 277, and 251 components were identified in the roots, fruit, and leaves, respectively. The fruit of A. oxyphylla had a higher abundance of flavonols. The roots of A. oxyphylla were enriched in flavonols and phenolic acids. The leaves of A. oxyphylla exhibited high contents of flavonols, phenolic acids, and tannins. Furthermore, network pharmacology analysis showed that flavonoids are the most important effectors in the fruit of A. oxyphylla and phenolic acids are the most important effectors in the roots and leaves. Moreover, the results suggested that the tissues of A. oxyphylla might play a role in the regulation of disease-related genes. The whole plant of A. oxyphylla is rich in natural drug components, and each tissue has high medicinal value. Therefore, comprehensive utilization of A. oxyphylla can greatly improve its economic value.

GC-MS analysis of bioactive components in six different crude extracts from the Soft Coral (Sinularia maxim) collected from Ras Mohamed, Aqaba Gulf, Red Sea, Egypt

GC-MS analysis of bioactive components in six different crude extracts from the Soft Coral (Sinularia maxim) collected from Ras Mohamed, Aqaba Gulf, Red Sea, Egypt

Quantitative analysis of bioactive components in walnut leaves by UHPLC-Q-Orbitrap HRMS combined with QAMS

Walnut leaves are rich in phenolic components with antibiotic and antioxidative effects. However, few studies have reported the quantitative analysis of active components in walnut leaf. In this study, a novel method for quantifying the active components in walnut leaves was developed by combining ultra-high performance liquid chromatography-hybrid quadrupole-Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) with quantitative analysis of multi-components by a single marker (QAMS). In total, 13 bioactive components were analyzed by a single marker, quercetin. To evaluate the accuracy of this method, an auxiliary quantification method with 13 reference standards was established and validated. The standard method differences (SMDs) of the quantification results between QAMS and the auxiliary method were lower than 20%, indicating that the QAMS method can accurately determine the active components in walnut leaves. This method can provide a reference to address the absence of reference standards for analyzing other foods and herbs.

Screening of cardioprotective diseases bioactive components from Danshen extracts and LC-MS analysis.

A rapid and efficient analysis and screening method is adopted for cell affinity capture coupled with HPLC-MS (CAC-HPLC-MS) analysis of bioactive components that have possible efficiency against cardiovascular diseases. This method involves affinity capture, concentration, and separation of bioactive components from Danshen library using oxidatively damaged endothelial cells induced by H2 O2 , as well as analysis and identification of targeted compounds with HPLC and MS. It combines the specific interaction between cell membrane receptors and bioactive components with the powerful analysis and identification function of HPLC-MS. The CAC-HPLC-MS method was also used for analysis and screening of bioactive components from crude extracts of Danshen. A total of 19 components were found to be bound to oxidatively damaged endothelial cells with seven of these identified. Existing literature confirms that these seven components have many activities related to cardioprotective diseases. Therefore, the combination of biological affinity capture with HPLC-MS should be regarded as an attractive method with great potential for rapid and efficient screening of bioactive components related to anti-cardiovascular diseases from natural product libraries.