Harapan Rain Forest (HRF) is is one of the lowland tropic that located in Sumatera. HRF has an area of 98.555 ha, of which 46.385 ha is located in Jambi Province and 52 170 ha in the Province of South Sumatra. Orchids is one of the indegenous species in HRF that’s endangered, it is caused by forest fire, illegal logging by the indegenous people (Suku Anak Dalam) and land convertion into palm oil plantation. So, it is important to know how many species of the Orchids that still hang on in the HRF and needed to protect by doing the research. This research aim is to do inventarization and conservation of the indegenous orchids from HRF. This research was arragned into two years, firstly: is inventarization by collecting samples, and the second years is to do conservation by tissue culture technique. Based on this research, the orchids have been collected from Harapan Rain Forest Jambi Province about 16 species. The species are dominated by Bulbophyllum, namely: Bulbophyllum Acutum, B. medusae, B. flavescence, B. sumatrana, Bulbophyllum sp1 and Bulbophyllum sp2. Only one orchid’s species that survive as an epiphyte and terresterial namely Gramatophyllum speciosumBl.. G. speciosum become endangered, because some of trees as a habitat are lossed by forest fire and human trafficking by explored without doing conservation. It is necessary to do the conservation of indigenous orchids for sustainable ecosystem in Harapan Rain Forest.