OBJECTIVES To compare the aerobic and anaerobic energy system contribution during 1500m running between collegiate untrained (UT) and endurance trained (ET) subjects.METHODS Five Asian UT (age: 23.8 ± 0.4 yrs, body fat %: 15.9 ± 5.7 %, height: 174.0 ± 4.1 cm, weight: 65.5 ± 4.1 kg) and 5 Asian ET male participants (age: 24.4 ± 3.9 yrs, body fat %: 12.9 ± 6.9 %, height: 169.4 ± 5.1 cm, weight: 60.6 ± 8.1 kg) participated in this study. Participants attended 3 sessions to determine their body composition, submaximal and maximal oxygen consumption (VO2max) test, 1500m track running session (RS) and 1500m treadmill RS. The maximally accumulated oxygen deficit (MAOD) method was used to calculate energy system contribution.RESULTS The times for the 1500m track run for the UT and ET were 428.0 ± 48.7 and 331.6 ± 14.0 seconds (p=0.004) respectively. There were no significant differences in VO2 between the 1500m track and treadmill RS indicating the participants ran to their personal best times for both trials. The mean VO2max(mL•kg-1•min-1) were significantly different between UT (45.1 ± 5.0) and ET participants (58.3 ± 2.2) (p=0.002). The mean relative contributions of the aerobic and anaerobic energy system during 1500m running were significantly different for the UT, 65.4 ± 7.0%, 34.6 ± 7.0 and ET, 75.7 ± 1.5%, 24.3 ± 1.5 % (p =0.011).CONCLUSIONS The point of equal contribution of the aerobic and the anaerobic systems occurred after thirty to forty seconds of intensive exhaustive running after which the aerobic contribution continues to increase while the anaerobic contribution decreases with increasing duration. By the end of 60th second of exhaustive running, the ET mean aerobic contribution is 71.5% compared to the UT’s 58.6%. This finding suggests a greater reliance on the aerobic energy system by the ET.