Amplitude Research Articles

Are performance and perceived fatigability dependent on the anchor scheme of fatiguing isometric tasks in men?

Ratings of perceived exertion (RPE) can be used to regulate exercise intensity. This study examined the effect of anchor scheme on performance fatigability and neuromuscular responses following fatiguing forearm flexion tasks. Twelve men (age 20.9±2.2 years; height 179.8±5.3 cm; body mass 80.2±9.9 kg) performed sustained, isometric forearm flexion tasks to failure anchored to RPE=6 (RPEFT) and the torque (TRQFT) that corresponded to RPE=6. Pre-test and post-test maximal voluntary isometric contractions (MVIC) were performed to quantify changes in the amplitude (AMP) and mean power frequency (MPF) of the electromyographic (EMG) and mechanomyographic (MMG) signals. Neuromuscular efficiency (NME) was calculated by dividing normalized torque by normalized EMG AMP. A dependent t-test was used to assess the mean difference for time to task failure (TTF). Repeated measures ANOVAs were used to compare mean differences for performance fatigability and normalized neuromuscular parameters. The RPEFT had a greater TTF than the TRQFT (P<0.001). MVIC and NME decreased from pre-test to post-test following the RPEFT and TRQFT (P<0.05) with no differences between anchor schemes. Following the TRQFT, normalized EMG MPF decreased from pre-test to post-test (P=0.004). Following the RPEFT, normalized MMG MPF increased from pre-test to post-test (P=0.021). There were no changes in normalized EMG AMP or MMG AMP (P>0.05). These findings indicated anchor scheme-specific neuromuscular responses and TTF, despite no difference in performance fatigability. Furthermore, performance fatigability was likely due to peripheral fatigue (based on normalized EMG MPF and NME) following the TRQFT, but peripheral and central fatigue (based on normalized MMG MPF and NME) following the RPEFT.

Blood-based protein biomarkers and red blood cell aggregation in pancreatic cancer.

Pancreatic ductal adenocarcinoma (PDAC) is highly malignant with a low 5-year survival rate. Blood biomarkers may be of value for the noninvasive diagnosis of pancreatic cancer. This study assessed blood-based biomarkers and disturbances in red blood cell aggregation associated with pancreatic cancer. We studied 61 patients who underwent pancreatic resection. Of these 61 patients, 46 patients had PDAC, and 15 patients had inflammatory tumours. Serum VEGF, hypoxia-inducible factor (HIF-1α), elastin-derived peptides (EDPs), total sialic acid (TSA) and resistin levels were measured. Red blood cell aggregation was assessed by a laser-assisted optical rotational cell analyser. VEGF (p < 0.000001), HIF-1α (p = 0.000002), resistin (p = 0.000349), EDP (p = 0.000089) and TSA (p = 0.000013) levels were significantly higher in the PDAC group than in the inflammatory tumour group. The aggregation index (AI), syllectogram amplitude (AMP) and threshold shear rate (γthr) were significantly higher in the PDAC group, whereas the aggregation half-time (t1/2) was lower than in the inflammatory tumour group. Multivariate analyses revealed that VEGF, TSA and EDP levels were variables that predicted PDAC. VEGF levels were the most powerful predictor of PDAC independent of CA 19-9 levels. The cut-off points for VEGF, TSA and EDP levels were 134.56 pg/ml, 109.11 mg/dl and 36.4 ng/ml, respectively, with sensitivities of 97.8%, 87% and 69.6%, respectively, and specificities of 86.7%, 86.7% and 93.3%, respectively. This study indicated that there are significant differences in blood-based biomarkers for differentiating between PDAC and inflammatory tumours of the pancreas. We also confirmed that PDAC is associated with the excessive aggregation of RBCs.

Unilateral and Bilateral Isokinetic Leg Extensions Exhibit No Differences in Neuromuscular Excitation Under Maximal and Fatiguing Conditions.

Abstract Anders, JPV, Neltner, TJ, Smith, RW, Arnett, JE, Housh, TJ, Schmidt, RJ, and Johnson, GO. Unilateral and bilateral isokinetic leg extensions exhibit no differences in neuromuscular activation under maximal and fatiguing conditions. J Strength Cond Res 38(3): 474–480, 2024—The bilateral deficit is a phenomenon where force production during a bilateral (BL) muscle action is less than the sum of force produced when the muscle action is performed unilaterally (UL) with both limbs. The purpose of this study was to compare peak force, electromyographic (EMG) amplitude (AMP), and mean power frequency (MPF) of the nondominant leg between UL and BL isokinetic leg extensions. Eleven recreationally trained men (mean ± SD; age = 20.8 ± 1.7 years; body mass = 84.0 ± 16.2 kg; height = 179.3 ± 7.2 cm) volunteered to participate in this study. The 2 test visits included UL and BL maximal isokinetic leg extensions at 180°·second−1 followed by a fatiguing task of either 50 UL or 25 UL followed immediately by 25 BL (ULBL) maximal, isokinetic leg extensions at 180°·second−1, in random order on separate days. The results demonstrated a significant (p = 0.015, = 0.460) bilateral deficit in force (UL = 56.29 ± 11.20 kg; BL = 50.32 ± 7.35 kg; d = 0.63; 95% CI [1.42, 10.52]) but no significant bilateral deficit in EMG AMP (p = 0.892, = 0.002). The peak force during the fatiguing task demonstrated a significant main effect for repetition (p &lt; 0.001, = 0.943) characterized by a reduced peak force from repetitions 20–50. Similarly, EMG MPF demonstrated a significant main effect for repetition (p &lt; 0.001, = 0.702) characterized by a reduced EMG MPF from repetitions 35–50. There were no significant effects (p = 0.102–0.387, = 0.096–0.203) for EMG AMP. These findings suggested that the bilateral deficit was not associated with a neuromuscular deficit and UL muscle actions may be used to potentiate force production of subsequent BL muscle actions.

Turbulent transport mechanism in the roughness sublayers over idealized urban areas and its implication to street-level ventilation

Turbulence in the roughness sublayer (RSL) is inhomogeneous compared with that in the inertial sublayer (ISL) of the atmospheric surface layer (ASL) over urban areas. Drag coefficient Cd, which measures aerodynamic roughness, is employed in this paper to examine how (idealized) urban morphology influences ASL dynamics and transport. Wind tunnel experiments are conducted to study the flows and turbulence in response to different configurations of (identical) roughness elements. Statistics, quadrant analysis, and tilt angle evidence the more efficient RSL transport over rougher surfaces even the winds are slower. Although the power spectra of streamwise u’’ and vertical w’’ fluctuating velocities are rather insensitive to Cd, their cospectrum shows a secondary peak at small motion scales λx (≤ 0.1δ where δ is the thickness of turbulent boundary layer) along with the primary peak at integral length scale Λx (≈ δ). It is thus suggested that, regardless of the turbulence intensity, RSL streamwise and vertical winds are more correlated, enhancing the transport. Amplitude (AM) and frequency (FM) modulations signify the positive correlation between RSL large and small motion scales which is amplified over rougher surfaces. Furthermore, RSL turbulence kinetic energy (TKE) production (entrainment) increases with decreasing (increasing) Cd, fostering the basic mechanism of street-level ventilation.

Improving Neural Network classification of native forest in New Zealand with phenological features

ABSTRACT Changes in Vegetation Indices (VIs) over time can describe vegetation phenology; however, it is not known which phenological features contribute most to land cover classification. Feature selection could potentially solve this problem. In this study, phenological feature importance and selection were evaluated by using two-year Sentinel-2 (S-2) data and single-date PlanetScope (PS) to classify a 50 km2 native podocarp forest in New Zealand. The study area was classified into nine classes. Single-date PS and S-2 data were fused to a base image with the same spatial resolution as PS and 8 bands containing spectral data from S-2; this image was used to produce 30 Vegetation Indices (VIs). Phenological features – amplitude (AMP) and phase (PH) were extracted from these VIs using time-series S-2 only, and harmonic analysis in Google Earth Engine. For accurately classifying forests and identifying the most important features, three classification scenarios (fused bands & VIs, fused bands & phenological features, fused bands & VIs & phenological features) were developed using a Neural Network. Variable Selection Using Random Forest (VSURF) was applied on these scenarios to evaluate the impact of feature selection. Results indicate that VSURF could reduce the time needed for the classification while maintaining a comparable level of accuracy. Phenological features improved accuracy from 90% to 94%, driven mostly by Red-Edge Triangulated Vegetation Index-AMP&PH, Normalised Near-Infrared-PH, Greenness Index-PH, Water Body Index-PH, Normalised Difference Vegetation Index-PH, Normalised Green-PH, Red-Edge Normalised Difference Vegetation Index-PH, Leaf Chlorophyll Content-AMP, and Simple Near-Infrared and Blue Ratio-PH. These features reflect changes in the structure, biochemical, and physiological characteristics of the canopy. A lack of ground-based measurements precluded an evaluation of the accuracy of these phenological aspects and an explanation of their distinctive contribution to the model. Overall, the findings show that specific phenological features can improve the classification of New Zealand’s indigenous podocarp forests.

The Role of Entrainment and Aperiodicity in Vibrotactile Discrimination

The extent to which entrainment – the phenomenon in neuroscience where neurons synchronize their firing patterns to external stimuli with rhythmic or periodic features – occurs in response to aperiodic or irregular flutter stimuli remains debatable. It has been proposed by Mountcastle et al. that information is processed in a periodic manner; however, more recently, Romo et al. challenged Mountcastle’s theory and proposed that flutter stimulus was encoded based on the rapidly adapting fibers, not the periodicity.1-5 Therefore, if Mountcastle et al. is correct, the brain should be more adept at discriminating frequency between periodic stimuli than between aperiodic stimuli; conversely, if Romo et al. is correct, the brain should be equally good at discriminating frequencies between periodic and aperiodic stimuli. In this study, a two-point vibrotactile stimulation device (The Brain Gauge, Cortical Metrics, USA) was used to stimulate digits II and III of 43 participants’ preferred hand to determine their difference limen (DL) for both amplitude (AD) and frequency (FD) discrimination tests, given aperiodic or periodic stimuli. The tests were performed in the following order: periodic AD, aperiodic AD, periodic FD, and aperiodic FD. The results suggest that the periodicity of the stimuli affects frequency discrimination more than amplitude discrimination and the participants were significantly better at discriminating stimuli with periodic frequencies than aperiodic frequencies. A paired, one-tailed t-test indicated that the subject DLs resulting from periodic stimuli were significantly different (p&lt;0.05) from that resulting from aperiodic stimuli for frequency discrimination, while it was not statistically significant for amplitude discrimination. The results were in favor of Mountcastle’s theory; however, more trials with a more controlled experimental environment may be needed to definitively conclude.

Neural processing of auditory temporal modulations in awake infants.

The amplitude modulation following response (AMFR) is the steady-state auditory response signaling phase-locking to slow variations in the amplitude (AM) of auditory stimuli that provide fundamental acoustic information. From a developmental perspective, the AMFR has been recorded in sleeping infants, compared to sleeping or awake adults. The lack of AMFR recordings in awake infants limits conclusions on the development of phase-locking to AM. Moreover, previous studies assessing phase-locking to AM using non-speech carriers have not included slow AM rates (<20 Hz), which are particularly important for speech processing. This study aimed at disentangling these issues by recording the AMFR with electroencephalography: in awake infants (3- and 10-month-olds) and awake young adults and for both slow and faster modulation rates (8 and 40 Hz). The AMFR was observable at 8 Hz at all ages (40%, 60%, and 33% of significant AMFR at 3 months, 10 months, and adults, respectively), but only adults showed reliable responses at 40 Hz (6% of significant AMFR at both 3 and 10 months, 100% in adults), thus, ruling out the possibility that sleep has a suppressing effect on the response. This pattern might be explained by developmental differences in the sources of neural processing of faster AM rates.

Acoustic Parameters in the Evaluation of Voice Quality of Choral Singers. Prototype of Mobile Application for Voice Quality Evaluation

Choral singers are among intensive voice users whose excessive vocal effort puts them at risk of developing voice disorders. The aim of the work was to assess voice quality for choral singers in the choir at the Polish-Japanese Academy of Information Technology. This evaluation was carried out using the acoustic parameters from the COVAREP (A Collaborative Voice Analysis Repository For Speech Technologies) repository. A prototype of a mobile application was also prepared to allow the calculation of these parameters. The study group comprised 6 male and 19 female choir singers. The control group consisted of healthy non-singing individuals, 50 men and 39 women. Auditory perceptual assessment (using the RBH scale) as well as acoustic analysis were used to test the voice quality of all the participants. The voice quality of the female choir singers proved to be normal in comparison with the control group. The male choir singers were found to have tense voice in comparison with the controls. The parameters which proved most effective for voice evaluation were Peak Slope and Normalized Amplitude Quotient.

Novel blood typing method by discrimination of hemagglutination and rouleaux using an erythrocyte aggregometer.

In pretransfusion blood typing, pretreatments such as centrifugation and suspension of red blood cells (RBCs) and mixing them with sufficient amounts of reagents are required, but these steps are time-consuming and costly. Aiming to develop a new blood typing method that requires no dilution and only a small amount of reagent, we attempted to determine blood type using syllectometry, an easy-to-use and rapid optical method for measuring the RBC aggregation that occurs when blood flow is abruptly stopped in a flow channel. Samples of whole blood from 20 healthy participants were mixed with antibody reagents for blood typing at mixing ratios of 2.5% to 10% and measured with a syllectometry device. Amplitude (AMP), one of the aggregation parameters, showed significant differences between agglutination and non-agglutination samples at mixing ratios from 2.5% to 10%. Although there were significant individual differences in aggregation parameters, calculation of AMP relative to that of blood before reagent mixing reduced the individual differences and enabled determination of blood type in all participants. This new method enables blood typing with a small amount of reagent, without the time-consuming and labor-intensive pretreatments such as centrifugation and suspension of RBCs.

Unilaterally Induced Quadriceps Fatigue during Sustained Submaximal Isometric Exercise Does Not Alter Contralateral Leg Extensor Performance.

This study investigated the effects of fatiguing unilateral exercise on the ipsilateral, exercised, and contralateral, non-exercised limb's post-exercise performance in males and females. Ten males and ten females performed a fatiguing, unilateral isometric leg extension at 50% maximal voluntary isometric contraction (MVIC) force. Prior to and immediately after the fatiguing tasks, MVICs were performed for the exercised and non-exercised limb, with surface electromyographic (sEMG) and mechanomyography (sMMG) amplitude (AMP) and mean power frequency (MPF) recorded from each limb's vastus lateralis. There were no fatigue-induced, sex-dependent, differences in time to task failure (p = 0.265) or ipsilateral performance fatigability (p = 0.437). However, there was a limb by time interaction (p < 0.001) which indicated decreases in MVIC force of the ipsilateral, exercised (p < 0.001), but not the contralateral, non-exercised limb (p = 0.962). There were no sex-dependent, fatigue-induced differences in neurophysiological outcomes between the limbs (p > 0.05), but there was a fatigue-induced difference in sEMG MPF (p = 0.005). To summarize, there were no differences in fatigability between males and females. Moreover, there was insufficient evidence to support the presence of a general crossover effect following submaximal unilateral isometric exercise. However, independent of sex, the neurophysiological outcomes suggested that competing inputs from the nervous system may influence the performance of both limbs following unilateral fatigue.

Power quality disturbance detection based on IEWT

With the continuous development of distributed generation, power quality problems are more complex. In order to ensure that the power quality of the active distribution network meets the requirements of users, it is of great significance to accurately obtain the power quality disturbance parameters. For the problem that the traditional empirical wavelet transform needs to manually select the number of frequency bands when analyzing unknown power quality disturbances, this paper proposes an improved empirical wavelet transform (IEWT) algorithm. The algorithm uses the Piecewise Cubic Hermite Interpolation (PCHIP) method and the improvement of the transition region to achieve adaptive frequency band division and noise interference reduction, and different amplitude (AM) and pitch (FM) components can be obtained. Then the Normalized Direct Quadrature (NDQ) algorithm and Singular Value Decomposition (SVD) algorithm are used to extract frequency, amplitude and time parameters of the AM-FM components. Simulation and experimental results show that the power quality disturbance parameters extracted by the proposed method are more accurate and less affected by noise.

Electromyographic and mechanomyographic responses of the biceps brachii during concentric and eccentric muscle actions to failure at high and low relative loads.

This study examined neuromuscular responses of the biceps brachii (BB) for concentric and eccentric muscle actions during bilateral, dynamic constant external resistance (DCER), reciprocal forearm flexions and extensions to failure at high (80% 1 repetition maximum [1RM]) and low (30% 1RM) relative loads. Nine women completed 1RM testing and repetitions to failure (RTF) at 30 and 80% 1RM. Electromyographic (EMG) and mechanomyographic (MMG) amplitude (AMP) and mean power frequency (MPF) signals were measured from the BB. Analyses included repeated measures ANOVAs (p < 0.05) and post-hoc pairwise comparisons with Bonferroni corrected alpha of p < 0.008 and p < 0.01 for between and within factor pairwise comparisons, respectively. EMG AMP and MPF were significantly greater for concentric than eccentric muscle actions, regardless of load or time. However, time course of change analysis revealed parallel increases in EMG AMP for concentric and eccentric muscle actions during the RTF trials at 30% 1RM, but no change at 80% 1RM. There were significant increases in MMG AMP during concentric muscle actions, but decreases or no change during eccentric muscle actions. EMG and MMG MPF decreased over time, regardless of muscle action type and loading condition. The greater EMG AMP and MPF values during concentric compared to eccentric muscle actions may reflect the difference in the efficiency characteristic of these muscle actions. The neuromuscular responses suggested that fatigue may be mediated by recruitment of additional motor units with lower firing rates during concentric muscle actions, and changes in motor unit synchronization during eccentric muscle actions.

Qualitative Evaluation of Intracranial Pressure Slopes in Patients Undergoing Brain Death Protocol

Due to the importance of not mistaking when determining the brain death (BD) diagnostic, reliable confirmatory exams should be performed to enhance its security. This study aims to evaluate the intracranial pressure (ICP) pulse morphology behavior in brain-dead patients through a noninvasive monitoring system. A pilot case-control study was conducted in adults that met the BD national protocol criteria. Quantitative parameters from the ICP waveforms, such as the P2/P1 ratio, time-to-peak (TTP) and pulse amplitude (AMP) were extracted and analyzed comparing BD patients and health subjects. Fifteen patients were included, and 6172 waveforms were analyzed. ICP waveforms presented substantial differences amidst BD patients when compared to the control group, especially AMP, which had lower values in patients diagnosed with BD (p < 0.0001) and the TTP median (p < 0.00001), but no significance was found for the P2/P1 ratio (p = 0.8). The area under curve for combination of parameters on the BD prediction was 0.77. In this exploratory study, noninvasive ICP waveforms have shown potential as a screening method in patients with suspected brain death. Future studies should be carried out in a larger population.

Flow Control around the UAS-S45 Pitching Airfoil Using a Dynamically Morphing Leading Edge (DMLE): A Numerical Study

This paper investigates the effect of the Dynamically Morphing Leading Edge (DMLE) on the flow structure and the behavior of dynamic stall vortices around a pitching UAS-S45 airfoil with the objective of controlling the dynamic stall. An unsteady parametrization framework was developed to model the time-varying motion of the leading edge. This scheme was then integrated within the Ansys-Fluent numerical solver by developing a User-Defined-Function (UDF), with the aim to dynamically deflect the airfoil boundaries, and to control the dynamic mesh used to morph and to further adapt it. The dynamic and sliding mesh techniques were used to simulate the unsteady flow around the sinusoidally pitching UAS-S45 airfoil. While the γ-Reθ turbulence model adequately captured the flow structures of dynamic airfoils associated with leading-edge vortex formations for a wide range of Reynolds numbers, two broader studies are here considered. Firstly, (i) an oscillating airfoil with the DMLE is investigated; the pitching-oscillation motion of an airfoil and its parameters are defined, such as the droop nose amplitude (AD) and the pitch angle at which the leading-edge morphing starts (MST). The effects of the AD and the MST on the aerodynamic performance was studied, and three different amplitude cases are considered. Secondly, (ii) the DMLE of an airfoil motion at stall angles of attack was investigated. In this case, the airfoil was set at stall angles of attack rather than oscillating it. This study will provide the transient lift and drag at different deflection frequencies of 0.5 Hz, 1 Hz, 2 Hz, 5 Hz, and 10 Hz. The results showed that the lift coefficient for the airfoil increased by 20.15%, while a 16.58% delay in the dynamic stall angle was obtained for an oscillating airfoil with DMLE with AD = 0.01 and MST = 14.75°, as compared to the reference airfoil. Similarly, the lift coefficients for two other cases, where AD = 0.05 and AD = 0.0075, increased by 10.67% and 11.46%, respectively, compared to the reference airfoil. Furthermore, it was shown that the downward deflection of the leading edge increased the stall angle of attack and the nose-down pitching moment. Finally, it was concluded that the new radius of curvature of the DMLE airfoil minimized the streamwise adverse pressure gradient and prevented significant flow separation by delaying the Dynamic Stall Vortex (DSV) occurrence.

Spindle wave in intracranial pressure signal analysis for patients with traumatic brain injury: A single-center prospective observational cohort study.

Objective: Intracranial pressure (ICP) monitoring is an integral part of the multimodality monitoring system in the neural intensive care unit. The present study aimed to describe the morphology of the spindle wave (a shuttle shape with wide middle and narrow ends) during ICP signal monitoring in TBI patients and to investigate its clinical significance. Methods: Sixty patients who received ICP sensor placement and admitted to the neurosurgical intensive care unit between January 2021 and September 2021 were prospectively enrolled. The patient's Glasgow Coma Scale (GCS) score on admission and at discharge and length of stay in hospital were recorded. ICP monitoring data were monitored continuously. The primary endpoint was 6-month Glasgow Outcome Scale-Extended (GOSE) score. Patients with ICP spindle waves were assigned to the spindle wave group and those without were assigned to the control group. The correlation between the spindle wave and 6-month GOSE was analyzed. Meanwhile, the mean ICP and two ICP waveform-derived indices, ICP pulse amplitude (AMP) and correlation coefficient between AMP and ICP (RAP) were comparatively analyzed. Results: There were no statistically significant differences between groups in terms of age (p = 0.89), gender composition (p = 0.62), and GCS score on admission (p = 0.73). Patients with spindle waves tended to have a higher GCS score at discharge (12.75 vs. 10.90, p = 0.01), a higher increment in GCS score during hospitalization (ΔGCS, the difference between discharge GCS score and admission GCS score) (4.95 vs. 2.80, p = 0.01), and a better 6-month GOSE score (4.90 vs. 3.68, p = 0.04) compared with the control group. And the total duration of the spindle wave was positively correlated with 6-month GOSE (r = 0.62, p = 0.004). Furthermore, the parameters evaluated during spindle waves, including mean ICP, AMP, and RAP, demonstrated significant decreases compared with the parameters before the occurrence of the spindle wave (all p < 0.025). Conclusion: The ICP spindle wave was associated with a better prognosis in TBI patients. Physiological parameters such as ICP, AMP, and RAP were significantly improved when spindle waves occurred, which may explain the enhancement of clinical outcomes. Further studies are needed to investigate the pathophysiological mechanisms behind this wave.

Load-Specific Performance Fatigability, Coactivation, and Neuromuscular Responses to Fatiguing Forearm Flexion Muscle Actions in Women.

Benitez, B, Dinyer-McNeeley, TK, McCallum, L, Kwak, M, Succi, PJ, and Bergstrom, HC. Load-specific performance fatigability, coactivation, and neuromuscular responses to fatiguing forearm flexion muscle actions in women. J Strength Cond Res 37(4): 769-779, 2023-This study examined the effects of fatiguing, bilateral, dynamic constant external resistance (DCER) forearm flexion on performance fatigability, coactivation, and neuromuscular responses of the biceps brachii (BB) and triceps brachii (TB) at high (80% 1 repetition maximum [1RM]) and low (30% 1RM) relative loads in women. Ten women completed 1RM testing and repetitions to failure (RTF) at 30 and 80% 1RM. Maximal voluntary isometric force was measured before and after RTF. Electromyographic (EMG) and mechanomyographic (MMG) amplitude (AMP) and mean power frequency (MPF) signals were measured from the BB and TB. Performance fatigability was greater (p < 0.05) after RTF at 30% (%∆ = 41.56 ± 18.61%) than 80% (%∆ = 19.65 ± 8.47%) 1RM. There was an increase in the coactivation ratio (less coactivation) between the initial and final repetitions at 30%, which may reflect greater increases in agonist muscle excitation (EMG AMP) relative to the antagonist for RTF at 30% than 80% 1RM. The initial repetitions EMG AMP was greater for 80% than 30% 1RM, but there was no difference between loads for the final repetitions. For both loads, there were increases in EMG MPF and MMG AMP and decreases in MMG MPF that may suggest fatigue-dependent recruitment of higher-threshold motor units. Thus, RTF at 30 and 80% 1RM during DCER forearm flexion may not necessitate additional muscle excitation to the antagonist muscle despite greater fatigability after RTF at 30% 1RM. These specific acute performance and neuromuscular responses may provide insight into the unique mechanism underlying adaptations to training performed at varying relative loads.

Application of the Ratings of Perceived Exertion-Clamp Model to Examine the Effects of Joint Angle on the Time Course of Torque and Neuromuscular Responses During a Sustained, Isometric Forearm Flexion to Task Failure.

Abstract Smith, RW, Housh, TJ, Anders, JPV, Neltner, TJ, Arnett, JE, Schmidt, RJ, and Johnson, GO. Application of the ratings of perceived exertion-clamp model to examine the effects of joint angle on the time course of torque and neuromuscular responses during a sustained, isometric forearm flexion to task failure. J Strength Cond Res 37(5): 1023–1033, 2023—The present study used the ratings of perceived exertion (RPE)-clamp model during a sustained, isometric forearm flexion task to failure to determine the effects of joint angle on torque and neuromuscular responses. Twelve college-aged women performed two 3-second maximal voluntary isometric contractions at elbow joint angles (EJ) of 75°, 100°, and 125° before sustained, isometric, forearm flexions anchored to RPE = 7 to task failure (defined as RPE &gt; 7, or the torque was reduced to zero) at EJ75, EJ100, and EJ125. The amplitude (AMP) and frequency (MPF) of the electromyographic (EMG) and mechanomyographic (MMG) signals from the biceps brachii were recorded. Repeated-measures ANOVAs and post hoc comparisons were used to examine differences across time and between joint angles for torque and neuromuscular parameters. A p-value ≤0.05 was considered significant. For each joint angle, there was a decrease (p &lt; 0.05) in torque across the sustained, isometric task. Collapsed across joint angle, there were decreases (p &lt; 0.001, = 0.378) in EMG AMP from 30 to 100% time to task failure. No changes were observed, however, for the other neuromuscular responses. The subjects voluntarily reduced torque to maintain RPE = 7 for each joint angle, and we hypothesize that RPE was maintained by afferent feedback from group III/IV motor neurons and corollary discharge (efferent copy of an internal signal that develops from central motor commands). The RPE-Clamp Model may be extended to athletes recovering from injury because a self-selected exercise intensity may be perceived as more enjoyable and promote adherence.

Coactivation does not contribute to fatigue-induced decreases in torque during reciprocal, isokinetic muscle actions

BACKGROUND: Studies of coactivation have typically utilized single movement isometric or isokinetic fatiguing muscle actions. OBJECTIVE: The purpose of the current study was to examine coactivation of the biceps brachii (BB) and triceps brachii (TB) in response to a maximal, reciprocal, isokinetic fatiguing task of the forearm flexors and extensors at slow (60∘/s) and moderate (180∘/s) isokinetic velocities in men. METHODS: Ten men (mean ± SD: age = 21.6 ± 1.3 years) completed 50 consecutive, maximal, reciprocal, isokinetic muscle actions of the right forearm flexors and extensors at 60 and 180∘/s. The amplitude (AMP) and mean power frequency (MPF) contents of the electromyographic (EMG) and mechanomyographic (MMG) signals from the BB and TB were recorded simultaneously throughout the fatiguing task. Repeated measures ANOVAs with Tukey post hocs were used to determine mean differences for the torque and neuromuscular parameters across repetitions. RESULTS: The torque analyses indicated greater fatigability at 180∘/s, compared to 60∘/s (p= 0.02). There were no significant changes in EMG AMP for either muscle during flexion or extension at 60∘/s (p&gt; 0.05). At 180∘/s, there were significant increases in agonist EMG AMP (p= 0.01 to 0.004), however, no changes in antagonist EMG AMP (p&gt; 0.05). For EMG MPF, there were significant decreases during flexion and extension (p&lt; 0.001 to p= 0.02) at both velocities, collapsed across muscle. There were no significant (p&gt; 0.05) changes across repetition for MMG AMP or MPF. CONCLUSIONS: This study indicated velocity-specific responses to fatigue, with a greater magnitude of fatigability at 180∘/s. Furthermore, despite increases in EMG AMP of the agonist muscles at 180∘/s only, it was not sufficient to alter the ratio of coactivation, likely due to common neural drive between muscles. Thus, the decreases in torque in the present study were not attributable to increases in coactivation.

Impact of Goreisan components on rat mesenteric collecting lymphatic vessel pumping.

Goreisan is a traditional herbal formulation with diuretic properties tested as a clinical therapeutic to alleviate lymphedema in Japan. The present study aimed to determine how Goreisan and its five different components affect lymphatic pump function. Mesenteric collecting lymphatics were isolated from anesthetized Sprague-Dawley rats and mounted on resistance-matched glass micropipettes in a 37°C physiological salt solution bath for studies. Diameter was continuously measured to obtain the following lymphatic pump parameters: contraction frequency (CF), end diastolic diameter (EDD), and end systolic diameter (ESD), contraction amplitude (AMP), ejection fraction (EF), and fractional pump flow (FPF). Goreisan and each of its components (Cinnamomi Cortex, Atractylodis Rhizoma, Alismatis Rhizoma, Polyporus, and Poria) were applied to the bath at concentrations of 1-30 μg/mL. The results show that while Goreisan causes no significant changes to lymphatic pumping, Alismatis Rhizoma and Polyporus each significantly reduce CF and FPF. In addition, rats that received oral administration of Goreisan and Alismatis Rhizoma for 1 week had elevated expression of VEGFR-3 in their mesenteric collecting lymphatics. Collectively, the results suggest that some components of Goreisan have a direct, rapid impact on lymphatic pumping. These findings provide new insights but also raise new questions about the therapeutic potential of Goreisan in patients with secondary lymphedema.

Simultaneous phase and amplitude modulation for dual-sensitivity profilometry of discontinuous objects.

Fringe projection profilometry (FPP) is a well-known technique for digitizing solids. In FPP, straight fringes are projected over a digitizing solid, and a digital camera grabs the projected fringes. The sensitivity of FPP depends on the spatial frequency of the projected fringes. The projected fringes as seen by the camera are phase modulated by the surface of the digitizing object; the demodulated phase is usually wrapped. If the digitizing object has discontinuities larger than the fringe period, the phase jumps are lost. To preserve large phase discontinuities, one must use very low spatial frequency (low-sensitivity) fringes. The drawback of low-sensitivity FPP is that the demodulated phase has low signal-to-noise ratio (SNR). Much higher SNR is obtained by projecting shorter wavelength, at the cost of obtaining wrapped phase. A way out of this problem is to use dual-wavelength FPP (DW-FPP). In DW-FPP, two sets of projected fringes are used, one with long wavelength and another with shorter wavelength. Due to harmonics and gamma distortion, in DW-FPP, one usually needs four phase-shifted fringes for each sensitivity. Here we are proposing to combine the two sensitivities simultaneously, one coded in phase (PM) and the other coded in amplitude (AM), in order to obtain phase and amplitude modulated (DW-PAM) fringes. The low-sensitivity phase is coded as AM of the DW-PAM fringes. The main advantage of DW-PAM fringes is that one reduces the number of phase-shifted fringes by half: instead of using eight phase-shifted fringes (four for low and four for high sensitivities), one would need only four DW-PAM fringes. Of course, if one wants to increase the harmonic rejection of the recovered phase, one may use a higher order phase-shifting algorithm (PSA).