Amounts Of Polyunsaturated Fatty Acids Research Articles

Maternal diet supplemented with African walnuts enhances cortico-hippocampal gene expression and histomorphology in rat offspring

ABSTRACT Background The brain is built up during pregnancy. How it functions afterwards depends on how the expectant mother's diet nourishes it. Walnuts contain significant quantities of polyunsaturated fatty acids (PUFAs) and bioactive phytochemicals, which enhance brain health and function even with advancing age. This study examined the effects of a walnut-enriched diet (WED) on corticohippocampal histoarchitecture and gene expression in rat offspring. Materials and methods Twenty-eight female adult Wistar rats (n= 7) averaging about 185 g in weight were used for this study. After mating, pregnant dams were split randomly into four groups: A (standard rat chow/control), B (WED from GD 0 – PND 21), C (WED from GD 0 – PND 1), D (WED from PND 1 – PND 21). Offspring of dams were sacrificed at adolescence (PND 35), with brain tissues of interest harvested for subsequent analyses. Results We observed no significant correlates in litter size, body, and brain weights across the experimental groups. Histomorphology revealed no distortion in cellular layering and delineation of cells in the PFC and dentate gyrus of both control and WED groups. Nissl staining intensity was enhanced in the offspring of dams exposed to WED versus the control, indicating improved proteostasis. Upregulated mRNA expression of DNMT3a, H2Ax, OPA1, and BDNF was observed in cortical and hippocampal tissues of WEDexposed offspring compared with the control group. Conclusion A diet enriched with African walnuts during early development induced changes predictive of cognitive improvements and enhanced stress-response signalling, plasticity, and neural resilience in rat offspring.

Wild Stinging Nettle (Urtica dioica L.) Leaves and Roots Chemical Composition and Phenols Extraction.

Stinging nettle (Urtica dioica L.) is an herbaceous plant that grows all over the world and is widely used as an edible and medicinal plant. Overall research results reveal that the chemical content and antioxidant activity of aerial parts and roots of stinging nettle depends on the growing region, soil, meteorological conditions (especially sunshine), collecting time, etc. The chemical composition of stinging nettle growing in Lithuania and the solid-liquid extraction efficiency of leaves and roots using different solvents were analysed. Additionally, we determined leaves phenols extraction efficiency using 96% methanol at different extraction conditions. Research results showed that a higher amount of crude fats, non-nitrogen extractives, and total carotenoids were in leaves, but the amount of crude proteins and ash did not differ significantly compared with roots. A higher amount of polyunsaturated fatty acids (PUFAs) and monounsaturated fatty acids (MUFAs) were detected in roots instead of leaves while saturated fatty acids (SFAs) were in leaves. The extraction results showed that the most effective solvent for total phenols and flavonoids in leaves was 96% methanol, for total phenols in roots was 50% methanol and 50% ethanol for total flavonoids in roots. The most effective temperature for the Urtica dioica L. leaves phenols extraction was 70 °C, while time does not have a significant influence. The present study's findings suggested that concentrated and binary solvents had different effects on the phenol's extraction efficiency from different stinging nettle parts and extraction temperature performed a key role instead of extraction time.

Butylated Hydroxytoluene (BHT) Improves the Post-Thaw Semen Quality in Low-Dose Sperm Cryopreservation in Murrah Buffalo Bull

BACKGROUND: Cryopreservation is an important technique for the long-term storage of semen for artificial insemination (AI). Buffalo spermatozoa are sensitive to cryopreservation procedures because of the presence of a high amount of polyunsaturated fatty acids in the plasma membrane. OBJECTIVE: To study the effect of different concentrations of BHT on the quality of Murrah buffalo bull semen for low-dose cryopreservation. MATERIAL AND METHODS: Semen was collected from four high fertile Murrah buffalo bulls (6 ejaculates each) using an artificial vagina. A total of 24 ejaculates were collected from each bull twice a week using an artificial vagina. Every sample was split into four parts: Control without additives; and three treatments with BHT at 0.5 mM, 1 mM or 2 mM. Semen was cryopreserved at low-dose sperm cryopreservation of 20, 15, 10 and 5 million sperm per aliquot after supplementation of BHT. Semen samples were evaluated for fresh, pre-freeze and post-thaw stages. RESULTS:There was a significant increase (p<0.05) in sperm quality parameters, such as progressive motility (%), viability (%), HOST response (%), acrosome integrity (%) and post-thaw motility, with the addition of 0.5-1 mM BHT. CONCLUSION: The addition of BHT in Murrah buffalo semen improves the low dose cryopreservation quality in a dose-dependent manner.

The processing of fish oil production waste into a lubricating additive

The search and development of rational uses of fatty waste formed in the production of fish oils is an urgent ecological problem of the fishing industry. Currently, this problem remains unresolved. Soap stock after refining fish oils and fatty foam after flotation treatment of industrial waste water from fish oil production contain a significant amount of polyunsaturated fatty acids and soaps. These components can be a basis for obtaining various technical products. It was found that soap stock and modified (saponified) fatty foam mass have antifriction and surface-active properties. This allows to determine the field of their use as a drilling mud lubricant. The study of the environmental safety of a new lubricant additive based on modified fatty foam mass made it possible to classify it as a low-hazard substance, determine the maximum permissible concentration in the water of fishery water bodies, and recommend it for use in the offshore oil and gas fields development.

Okara Waste as a Substrate for the Microalgae Phaeodactylum tricornutum Enhances the Production of Algal Biomass, Fucoxanthin, and Polyunsaturated Fatty Acids

Despite the rich nutritional content of okara, the majority remains underutilized and discarded as food waste. In this study, solid-state fermentation of okara with food-grade fungi was performed to extract and solubilize any remnant nutrients locked within the lignocellulosic matrix to produce a nutrient-rich okara fermentate. Fermented okara media (FOM) was used as the sole nutrient source for growing marine diatom, Phaeodactylum tricornutum. Results have shown a two-fold increase in biomass production when grown on FOM (0.52 g L−1) as compared with conventional Guillard’s F/2 media (0.25 g L−1). Furthermore, cellular fucoxanthin content was enhanced significantly by two-fold to reach a final concentration of 15.3 mg g−1 compared to 7.3 mg g−1. Additionally, a significantly higher amount of polyunsaturated fatty acid (PUFA) was produced, particularly eicosapentaenoic acid (EPA) which yield has increased by nearly three-fold. Metabolomics analysis of intracellular contents in fermented okara culture revealed a significantly enhanced accumulation of nitrogenous metabolites, alongside the decrease in sugar metabolites as compared to F/2 culture, thus indicating metabolic flux towards pathways involved in cellular growth. This study demonstrated an innovative and low-cost strategy of using fermented okara as a nutritious substrate for achieving a sustainable media replacement for high density algal growth with a simultaneous enhancement of production in highly valued nutraceuticals, including fucoxanthin and EPA.

Study of adipose tissue of Kemerovo piglets: Detection of beige adipocytes

Animals have two types of adipose tissue differing in structure and function: white (WAT) and brown (BAT). Beige adipose tissue (BeAT) is a result of WAT browning, when beige adipocytes appear between white adipocytes in response to exposure to cold, diet or physical activity. BeAT shares morphological and biochemical characteristics with BAT, is thermogenic and dissipates energy in the form of heat, unlike WAT, which is responsible for energy storage. Pigs do not have classic BAT, and modern breeds are sensitive to cold. There is literature information that BeAT has been found in cold-resistant pigs. The aim of the work was to study adipose tissue of piglets of the Kemerovo cold-resistant breed under growing conditions in the cold season and to identify the localization of BeAT. Histological studies have shown two types of adipocytes in subcutaneous fat samples (lateral, backfat and axillary): white, with one large fat droplet, and beige, multilocular. Larger cells were detected in backfat fat (69.36±12.98 µm) compared with lateral (53.25±9.27 µm) and axillary fat (45.94±8.29 µm). Only WAT with a diameter of 35.69±6.96 µm was present in the internal perirenal fat. Raman spectroscopy was used to evaluate the overall fatty acid profile of the tested samples. The main peaks were noted in all samples: 970 cm-1 (=C-H out-of-plane bend), 1266/1272 cm-1 (=C-H symmetrical rock) and 1655 cm-1 (C=C stretching) responsible for unsaturated bonds, and signals at 1297/1301 cm-1 (CH2 twisting), 1430/1460 cm-1 (CH2 symmetrical deformation (scissoring)) and 1735/1746 cm-1 (C=O stretching) corresponding to saturated bonds or ester groups. Internal perirenal fat contained the largest number of saturated fatty acid bonds, subcutaneous axillary fat — the smallest. The average intensity of the peaks was 0.4801010 and 0.639995, respectively. According to the results of gas chromatography, the largest amount of polyunsaturated fatty acids was noted in the subcutaneous fat samples: 20.199 in backfat fat, 21.749 in lateral and 20.436 in axillary fat compared to 18.636 in internal fat. Activation of beige cells in Kemerovo pigs under cold exposure, according to the authors, plays a crucial role in the heat balance, allowing them to tolerate cold without severe shivering. The study of the BeAT formation is of great practical importance for changing energy metabolism and increasing thermogenesis in newborn piglets by genome editing, as well as for improving the quality of pig’s fat.

Shark liver and flaxseed oils as alternatives to soybean oil in the lipid quality of Japanese quail eggs

PurposeThe objective of this study was to assess the incorporation of polyunsaturated omega 3 and omega 6 fatty acids in the eggs of Japanese quails (Coturnix coturnix japonica) fed with soybean, flaxseed and shark liver oils, as well as to assess their effects on the chemical composition and lipid quality of the yolks.Design/methodology/approachThe total of 252 units of quails were distributed in a completely randomized design with seven treatments and six replications, totaling 42 experimental design units. The sources of oils and their inclusions in the experimental diet were 2% of soybean oil (S), 2% of flaxseed oil (L) and 2% shark liver oil (T), obtained from the species Carcharhinus acronotus, and the mixtures of 1% S + 1% L (SL); 1% S + 1% T (ST); 1% L + 1% T (LT) and 0.67% S + 0.67% L + 0.67% T (SLT).FindingsThe proximate composition of the egg yolk was not changed to the supplementation of lipid sources added in quail diet. The total replacement of soybean oil by shark liver oil reduced the cholesterol content in the yolk by 19% (T = 282.97 mg cholesterol/100 g egg) and the addition of flaxseed oil produced eggs with greater amount of polyunsaturated fatty acids (PUFAs) (LS = 18.32%). The treatments with flaxseed oil and its mixtures (soybean oil + flaxseed oil and flaxseed oil + shark liver oil) effectively contributed to reducing the ω-6:ω-3 ratio and enriching the quail eggs with PUFAs, especially linolenic acid (18: 3n3c) and EPA (20: 5n3c).Originality/valueThus, manipulation in the diet of quails enriched the content of essential PUFAs and reduced the cholesterol content in the yolk. The flaxseed oil was the lipid source that influenced to improve the quality of the egg yolk. These results have practical applicability for the quail egg industry.

Use of distillery by-products asTenebrio molitor mealworm feed supplement

Agricultural and industrial waste represent valuable starting materials to create novel products with economic value added. Winery industry represents an important economic sector in Italy, which produces tons of by-products every year. Global warming and the increasing demand for food and feed led us to analyse the nutraceutical properties of distillery by-products as possible supplements to feedTenebrio molitor larvae (TML). Grape pomace (GP) and grape marcs (GM), grape skin pulp, grape seeds and winery waste sludge were analysed for their antioxidant activity and fatty acid (FA) profile. Even if subjected to multiple processing, by-products had an important content of antioxidant compounds, in particular polyphenols, flavonols, flavonoids and condensed tannins. Moreover, the high amount of polyunsaturated fatty acids and the low percentage of saturated fatty acids found make them useful feed supplements. Herein has been disclosed that their use as TML feed material was well tolerated over TML development, with a significant mean weight gain respect to control of about 25% and no effect on survival rate. Interestingly, total antioxidant activity and FA profile ameliorate significantly, suggesting that distillery by-products can be used to extend the shelf-life of TML and ameliorate their nutraceutical properties, with possible application in controlled dietary regimens. This work confirmed that by-products largely produced in Europe can be used as TML feed materials, simplifying waste management and reducing rearing costs.

Dominant phytoplankton groups as the major source of polyunsaturated fatty acids for hilsa (Tenualosa ilisha) in the Meghna estuary Bangladesh

The tropical estuarine ecosystem is fascinating for studying the dynamics of water quality and phytoplankton diversity due to its frequently changing hydrological conditions. Most importantly, phytoplankton is the main supplier of ω3 polyunsaturated fatty acids (PUFA) in the coastal food web for fish as they could not synthesize PUFA. This study evaluated seasonal variations of water quality parameters in the Meghna River estuary (MRE), explored how phytoplankton diversity changes according to hydro-chemical parameters, and identified the major phytoplankton groups as the main source of PUFA for hilsa fish. Ten water quality indicators including temperature, dissolved oxygen, pH, salinity, dissolved inorganic nitrogen (DIN = nitrate, nitrite, ammonia) and phosphorus, dissolved silica and chlorophyll-a were evaluated. In addition, phytoplankton diversity was assessed in the water and hilsa fish gut. Principal component analysis (PCA) was used to analyze the spatio-temporal changes in the water quality conditions, and the driving factors in the MRE. Four main components were extracted and explained 75.4% variability of water quality parameters. The most relevant driving factors were dissolved oxygen, salinity, temperature, and DIN (nitrate, nitrite and ammonia). These variabilities in physicochemical parameters and dissolved inorganic nutrients caused seasonal variations in two major groups of phytoplankton. Peak abundance of Chlorophyta (green algae) occurred in water in nutrient-rich environments (nitrogen and phosphorus) during the wet (36%) season, while Bacillariophyta (diatoms) were dominant during the dry (32%) season that depleted dissolved silica. Thus, the decrease of green algae and the increase of diatoms in the dry season indicated the potential link to seasonal changes of hydro-chemical parameters. The green algae (53.7%) were the dominant phytoplankton group in the hilsa gut content followed by diatoms (22.6%) and both are contributing as the major source of PUFAs for hilsa fish according to the electivity index as they contain the highest amounts of PUFAs (60 and 28% respectively).

English

The study analysed the fatty and amino acid compositions of chia seeds and the physicochemical characterisation of chia seeds oil. The quantification of amino and fatty acids was done using high-performance liquid chromatography (HPLC) and gas chromatography equipped with a flame ionisation detector (GC-FID). The amino acids from chia seeds were leucine, valine, phenylalanine, lysine, isoleucine, threonine, histidine, and methionine with 1.63, 1.37, 1.34, 1.14, 0.98, 0.98, 0.88 and 0.81 g/100 g, respectively. Saturated and unsaturated fatty acids were 3.95 and 30.48%, respectively. Meanwhile, the mean ratio of Omega 6/Omega 3 fatty acids was 0.333. The established physicochemical characteristics of CSO were acid value 2.63 mg KOH/g, peroxide value 6.23 meq active oxygen/kg, iodine value 200.721 g I2/100 g, saponification value 193.345 mg KOH/g, refractive index 1.454 at 40°C relative density 0.8824 g/cm3 at 20°C and specific gravity 0.882 at 40°C. The fatty acid and amino acid profiling of chia seeds revealed the presence of an appreciable amount of polyunsaturated fatty acids (PUFA) and essential amino acids. The physicochemical characteristics of CSO constitute a drying oil category that requires timely usage and proper storage condition to reduce rancidity from air or light exposure.   Key words: Chia seeds, chia seeds oil, amino acids, fatty acids, physicochemical characteristics.

Valorization Potential of Oil Industry By-Products as Sources of Essential Fatty Acids

The oil industry generates a high number of by-products which have the potential to be used in food formulation after they are properly treated. The aim of this study was to evaluate the nutritional potential of 14 different vegetable by-products from the oil industry (flour, meals, and groats) in terms of fatty acid composition and to investigate the effect of theoretical fortification of the bakery products with by-products high in ω-3. Results showed that some of the analyzed samples contain significant amounts of polyunsaturated fatty acids. Organic walnut flour and grape seed flour had a linoleic acid content higher than 60%. By-products obtained from thistle and sesame had a linoleic acid content ranging between 47.32% and 54.72%. Higher linolenic content was obtained for hemp flour (33.22%), red grape seeds meals (33.62%), and golden flax meals (64.65%). By theoretical fortification of wheat and wholemeal flours with high ω-3 by-products in a proportion of 5, 10, and 15%, the ω-3 content increased (2.2- to 22-fold higher) while the ω-6/ω-3 ratio decreased from 5.22 to 0.52–2.86 for wheat flour, and from 16.80 to 0.81–5.53 for wholemeal flour. These vegetable by-products from the oil industry could represent valuable sources of essential fatty acids for the food application, representing valuable ingredients that could be added for the production of functional foods.

A review on the sustainable procurement of microalgal biomass from wastewaters for the production of biofuels

The feasibility of microalgal biomass as one of the most promising and renewable sources for the production of biofuels is being studied extensively. Microalgal biomass can be cultivated under photoautotrophic, heterotrophic, photoheterotrophic, and mixotrophic cultivation conditions. Photoautotrophic cultivation is the most common way of microalgal biomass production. Under mixotrophic cultivation, microalgae can utilize both organic carbon and CO2 simultaneously. Mixotrophic cultivation depicts higher biomass productivity as compared to photoautotrophic cultivation. It is evident from the literature that mixotrophic cultivation yields higher quantities of polyunsaturated fatty acids as compared to that photoautotrophic cultivation. In this context, for economical biomass production, the organic carbon of industrial wastewaters can be valorized for the mixotrophic cultivation of microalgae. Following the way, contaminants’ load of wastewaters can be reduced while concomitantly producing highly productive microalgal biomass. This review focuses on different aspects covering the sustainable cultivation of different microalgal species in different types of wastewaters.

Effects of dietary substitution of peanut meal for soybean meal on egg production, egg quality, oxidative status, and yolk fatty acid profile in laying ducks

There is an urgent need to evaluate and introduce alternatives for the unsustainable and traditional feed stuffs in poultry. This study evaluated the effects of graded replacement of soybean meal (SBM) by peanut meal (PNM) on egg production, egg quality, oxidative status, and yolk fatty acid profile in laying ducks. In total, 360 Longyan ducks aged 21 weeks were allocated to five treatments, each containing six replicates of 12 birds. Birds were fed the diets containing PNM replacing 0 (control), 25, 50, 75 or 100% of SBM for 16 weeks. With the increase of PNM level, egg production was improved (quadratic, P < 0.05), egg weight and feed consumption were decreased, feed conversion ratio and egg mass were impaired at 100% PNM, and yolk colour was enhanced (quadratic, P < 0.05). Plasma malondialdehyde concentration was increased at 100% PNM, while plasma glutathione concentration was decreased (quadratic, P < 0.01) as PNM substitution increased. Total cholesterol content in yolk decreased (P < 0.01) in response to increased PNM substitution. The contents of saturated fatty acids C20:0 and C22:0 in yolk increased (linear or quadratic, P < 0.05) with increased PNM substitution, but the contents of saturated fatty acid C18:0, monounsaturated fatty acid C22:1, and polyunsaturated fatty acids C18:3n-3 and C22:6n-3 in yolk were decreased (linear, P < 0.05) as PNM replacement increased. The contents in yolk of polyunsaturated fatty acid C18:2n-6, C20:2n-6, C20:3n-6, C20:4n-6, and total amount of polyunsaturated fatty acids increased with the highest contents obtained at 75% PNM substitution. The ratio between n-6 and n-3 increased (linear, P < 0.05) as PNM substitution increased. The transcript abundance in liver of peroxisome proliferators-activated receptors γ and fatty acid synthase showed quadratic (P < 0.05) responses with PNM replacement, with the highest expression of both genes being obtained with 75% substitution with PNM. Replacement of dietary SBM with 100% PNM decreased egg production and antioxidant capacity and increased the ratio of omega fatty acid in yolk between n-6 and n-3 in laying ducks. The obtained results indicate that PNM can be used to replace up to 75% of SBM in the diet of laying ducks without negative effects on the egg-laying production or egg quality. The regression model indicated that the maximal egg mass was obtained at no more than 67.6% replacement of SBM with PNM in the diet of laying ducks.

Lipidome analysis and characterization of Buglossoides arvensis acyltransferases that incorporate polyunsaturated fatty acids into triacylglycerols

Buglossoides arvensis is a burgeoning oilseed crop that contains an unique combination of ω-3 and ω-6 polyunsaturated fatty acids (PUFA), constituting ~80–85% of seed triacylglycerols (TAGs). To uncover the critical TAG biosynthetic pathways contributing for high PUFA accumulation, we performed lipidome of developing seeds and characterized acyltransferases involved in the final step of TAG biosynthesis. During seed development, distribution of lipid molecular species in individual lipid classes showed distinct patterns from an early-stage (6 days after flowering (DAF)) to the middle-stage (12 and 18 DAF) of oil biosynthesis. PUFA-containing TAG species drastically increased from 6 to 12 DAF. The expression profiles of key triacylglycerol biosynthesis genes and patterns of phosphatidylcholine, diacylglycerol and triacylglycerol molecular species during seed development were used to predict the contribution of diacylglycerol acyltransferases (DGAT1 and DGAT2) and phospholipid: diacylglycerol acyltransferases (PDAT1 and PDAT2) to PUFA-rich TAG biosynthesis. Our analysis suggests that DGATs play a crucial role in enriching TAGs with PUFA compared to PDATs. This was further confirmed by fatty acid feeding studies in yeast expressing acyltransferases. BaDGAT2 preferentially incorporated high amounts of PUFAs into TAG, compared to BaDGAT1. Our results provide insight into the molecular mechanisms of TAG accumulation in this plant and identify target genes for transgenic production of SDA in traditional oilseed crops.

Comparative Genomics of Mortierellaceae Provides Insights into Lipid Metabolism: Two Novel Types of Fatty Acid Synthase.

Fungal species in the family Mortierellaceae are important for their remarkable capability to synthesize large amounts of polyunsaturated fatty acids, especially arachidonic acid (ARA). Although many genomes have been published, the quality of these data is not satisfactory, resulting in an incomplete understanding of the lipid pathway in Mortierellaceae. We provide herein two novel and high-quality genomes with 55.32% of syntenic gene pairs for Mortierella alpina CGMCC 20262 and M. schmuckeri CGMCC 20261, spanning 28 scaffolds of 40.22 Mb and 25 scaffolds of 49.24 Mb, respectively. The relative smaller genome for the former is due to fewer protein-coding gene models (11,761 vs. 13,051). The former yields 45.57% of ARA in total fatty acids, while the latter 6.95%. The accumulation of ARA is speculated to be associated with delta-5 desaturase (Delta5) and elongation of very long chain fatty acids protein 3 (ELOVL3). A further genomic comparison of 19 strains in 10 species in three genera in the Mortierellaceae reveals three types of fatty acid synthase (FAS), two of which are new to science. The most common type I exists in 16 strains of eight species of three genera, and was discovered previously and consists of a single unit with eight active sites. The newly revealed type II exists only in M. antarctica KOD 1030 where the unit is separated into two subunits α and β comprised of three and five active sites, respectively. Another newly revealed type III exists in M. alpina AD071 and Dissophora globulifera REB-010B, similar to type II but different in having one more acyl carrier protein domain in the α subunit. This study provides novel insights into the enzymes related to the lipid metabolism, especially the ARA-related Delta5, ELOVL3, and FAS, laying a foundation for genetic engineering of Mortierellaceae to modulate yield in polyunsaturated fatty acids.

Effects of the Replacement of Pork Backfat with High Oleic Sunflower Oil on the Quality of the "Chorizo Zamorano" Dry Fermented Sausage.

The “Chorizo Zamorano” dry fermented sausage is a traditional Spanish product which is highly appreciated by consumers. This paper studies the reformulation of this product in order to improve its lipid composition and its fatty acid profile and to reduce its fat content. To achieve this, the fat used in the production of the product was partially replaced with high oleic sunflower oil in proportions of 12.5%, 20%, and 50% of the total fat content. Proximate analysis, fatty acid profiles, lipid oxidation, and sensory analysis were studied. The replacement of fat with oil showed a significant effect on the evolution of the parameters analyzed during ripening in all cases. The batches with sunflower oil presented higher levels of monounsaturated fatty acids (MUFA) and lower levels of saturated fatty acids (SFA) and a similar amount of polyunsaturated fatty acids (PUFA) to the control products. The replacement of up to 20% of oil showed no significant differences for most of the physicochemical and sensory parameters analyzed at the end of the ripening.

The High ‘Lipolytic Jump’ of Immobilized Amano A Lipase from Aspergillus niger in Developed ‘ESS Catalytic Triangles’ Containing Natural Origin Substrates

Lipase Amano A from Aspergillus niger (AA-ANL) is among the most commonly applied enzymes in biocatalysis processes, making it a significant scientific subject in the pharmaceutical and medical disciplines. In this study, we investigated the lipolytic activity of AA-ANL immobilized onto polyacrylic support IB-150A in 23 oils of natural origin containing various amounts of polyunsaturated fatty acids (PUFAs) and monounsaturated fatty acids (MUFAs). The created systems were expressed as an ‘ESS catalytic triangle’. A distinct ‘jump’ (up to 2400%) of lipolytic activity of immobilized AA-ANL compared to free lipase and hyperactivation in mostly tested substrates was observed. There was a ‘cutoff limit’ in a quantitative mutual ratio of ω-PUFAs/MUFAs, for which there was an increase or decrease in the activity of the immobilized AA-ANL. In addition, we observed the beneficial effect of immobilization using three polyacrylic supports (IB-150A, IB-D152, and IB-EC1) characterized by different intramolecular interactions. The developed substrate systems demonstrated considerable hyperactivation of immobilized AA-ANL. Moreover, a ‘lipolytic jump’ in the full range of tested temperature and pH was also observed. The considerable activity of AA-ANL-IB-150A after four reuse cycles was demonstrated. On the other hand, we observed an essential decrease in stability of immobilized lipase after 168 h of storage in a climate chamber. The tested kinetic profile of immobilized AA-ANL confirmed the decreased affinity to the substrate relative to lipase in the free form.

The influence of seed oats and flax meal in the diet of Yakut horses in winter on metabolic and energy indicators

The article presents the results of studies on the influence of pasture on the growth of natural and seeded herbage and the feeding of flax meal on metabolic and energy indicators in horses of the Yakut breed in winter. It was found that the seeds of the sown herbage (winter-green mass of oats) were distinguished by a high content of nutrients and energy-intensive substances. Significant differences were found for dry matter 63.7 ± 0.13% and for protein - 9.0 ± 0.14% (P³ 0.95). The content of carotene in the winter-green mass of oats is 104.6% higher than in the natural herbage (28.4 ± 0.76%) and amounted to 133.0 ± 0.44% (P ³0.999). At the same time, the largest amounts of polyunsaturated fatty acids were identified. Thus, the amount of polyunsaturated fatty acids in the oatmeal of the sown herbage (winter-green mass of oats) was 40.7%, which was 25.06% higher than the amount of fatty acids of the natural herbage (15.65 ± 0.18%). The high content of polyunsaturated fatty acids in the winter-green mass of oats contributed to a higher digestion of dry (66.5%) and organic (66.1%) matter, crude protein (70.6%), crude fiber (65.4%), crude fat (51.5%) and nitrogen-free active substances (66.7%) than with horse breeding on from the loss of natural herbage and, accordingly, higher use of exchange energy per 1 head per day (145.4 ± 3.32 MJ) and per 100 kg of live weight (36.53 ± 2.48 MJ). The inclusion of flax meal in the winter diet of horses had a positive effect on the digestibility of feed in the horse's body, in addition, it positively affected the indicators of energy metabolism. Significantly high digestibility coefficients in horses of the experimental group were noted for crude protein - by 3.34% (70.85 ± 0.19%) (P ³0.99), for crude fat - by 14.3% (68.59 ± 0.64%) and for nitrogen-free active substances - by 3.09% (77.71 ± 1.13%) (P³0.999). The high digestibility of the main nutrients led to a high content of metabolic energy in 1 kg of dry matter when flaxseed cake was included in the diet (10.1 MJ) than in the hay-oat diet (9.5 MJ), (P³0.95). The results obtained by us indicate the need for widespread use of seeded herbage during pasture and the inclusion of linseed meal as a source of polyunsaturated fatty acids in winter for horses of the Yakut breed.

Deep eutectic solvents for the extraction of fatty acids from microalgae biomass: Recovery of omega-3 eicosapentaenoic acid

Microalgae are a vast group of autotrophic microorganisms whose metabolic diversity makes them a natural source of valuable organic compounds such as lipids, carbohydrates, proteins, vitamins, and bioactive molecules. Several microalgae species contain notable amounts of polyunsaturated fatty acids, particularly eicosapentaenoic acid (EPA), which is an important alpha-linolenic acid derivative for human health. Conventional methods are considered effective at recovering total lipids from microalgae, however, they imply the use of large volumes of organic solvents such as methanol and chloroform, which are toxic and pose environmental risks. Thus, it is necessary to find new methods involving sustainable and green extracting phases. Deep eutectic solvents (DES) are renewable compounds often formed, but not exclusively, by quaternary ammonium salts and non-hydrated metal halides. Due to their availability, low cost, biodegradability, and environmental friendliness, DES are a promising alternative to organic solvents in extraction processes. This work assesses the efficiency of several DES phases for the extraction of fatty acids from the microalgae Nannochloropsis gaditana with a special interest in the recovery of EPA. The tested phases include mixtures containing choline chloride, lactic acid, ethylene glycol, and sodium acetate. Their performances were compared to those provided by conventional methods based on the use of organic solvents. Specifically, an in-situ transesterification process based on methanol with 10 %v/v of HCl was optimized in terms of temperature, time, and catalyst amount to be used as a reference. The results show that several of the tested eutectics such as choline chloride-ethylene glycol were capable of matching and even outperforming the best results obtained for EPA, with 104 % of extracted EPA methyl ester as the percentage of the mass obtained with HCl-methanol. The extraction capacity of DES was also improved by microalgae biomass pretreatment using ultrasonic and NaCl-based methods in a further stage. In the case of EPA extraction, and under optimal conditions, DES were capable of recovering over 18 % more quantity than the obtained with HCl-methanol. These results demonstrate that DES are effective at both recovering total fatty acids from pretreated biomass and at selectively recovering EPA using both unpretreated and pretreated biomass.

Statistical optimization of phytol and polyunsaturated fatty acid production in the Antarctic microalga &lt;italic&gt;Micractinium variabile&lt;/italic&gt; KSF0031

Polar microorganisms produce physiologically active substances to adapt to harsh environments, and these substances can be used as biomedical compounds. The green microalga Micractinium variabile KSF0031, which was isolated from Antarctica, produced phytol, a natural antimicrobial agent. Furthermore, several polyunsaturated fatty acids (PUFAs), including omega-3, exhibit antioxidant properties. Here statistical methods (Plackett-Burman design and Box- Behnken design) were used to optimize the culture medium of KSF0031 to improve biomass production, and K2HPO4, MgSO4·7H2O, and ammonium ferric citrate green (AFCg) were selected as significant components of the culture medium. Changes in the concentration of K2HPO4 and MgSO4·7H2O as positive factors and AFCg as a negative factor affected cell growth to a remarkable degree. The biomass production in a 100 L culture using the optimized medium for 24 d at 18°C was improved by 37.5% compared to that obtained using the original BG-11 medium. The quantities of PUFAs and phytol obtained were 13 mg g-1 dry cell weight (DCW) and 10.98 mg g-1 DCW, which represent improved yields of 11.70% and 48.78%, respectively. The results of this study could contribute to an improved production of phytol and fatty acids from Antarctic microalgae in the biomedical industry.