Amount Of Novel Information Research Articles

Novel and emerging seed science research from early to middle career researchers at the Australasian Seed Science Conference, 2021

Seed science is a vital field of research that contributes to many areas of knowledge in fundamental ecology and evolution, as well as in applied areas of food production, and the conservation and restoration of native plants. A large amount of novel information, technologies and processes in seed science research are being produced and developed by early to middle career researchers (EMCRs) in academic, government and private science sectors. This breadth and novelty of research by EMCRs was evident at the second Australasian Seed Science Conference held online in September 2021. EMCRs represented almost one-third of the presenting delegates at the conference and covered research in areas including functional seed trait relationships, responses of seed traits and germination to environmental change, managing seeds in ex-situ seed and germplasm collections and using seeds as food sources. As future environmental, social and economic challenges arise, EMCR seed scientists will be at the forefront of emerging fundamental ecological and evolutionary seed science knowledge, as well as the development of technologies and processes for the conservation of native species, the utilisation of seeds in agriculture and food production, and many new ideas yet to be discovered.

A clinician’s dilemma: what should be communicated to women with oncogenic genital HPV and their partners regarding the risk of oral viral transmission?

Head and neck cancer, the sixth most common cancer worldwide, account for about 1 out of 20 malignant tumors. In recent years a reduction in the incidence of cervical cancer, but a concomitant major increase in the incidence of HPV-mediated oropharyngeal cancer caused by orogenital HPV transmission has been observed. Consequently, in wealthy countries oropharyngeal squamous-cell carcinomas (OPSCC) is now the most frequent HPV-related cancer, having overtaken cervical cancer. Without effective medical interventions, this incidence trend could continue for decades. As no specific precursor lesion has been consistently identified in the oral cavity and oropharynx, HPV vaccination is the logical intervention to successfully counteract also the rising incidence of OPSCCs. However, HPV vaccine uptake remains suboptimal, particularly in males, the population at higher risk of OPSCC. Alternative primary prevention measures, such as modifications in sexual behaviors, could be implemented based on knowledge of individual genital HPV status. Until recently, this information was not available at a population level, but the current gradual shift from cytology (Pap test) to primary HPV testing for cervical cancer screening is revealing the presence of oncogenic viral genotypes in millions of women. In the past, health authorities and professional organizations have not consistently recommended modifications in sexual behaviors to be adopted when a persistent high-risk HPV cervicovaginal infection was identified. However, given the above changing epidemiologic scenario and the recent availability of an immense amount of novel information on genital HPV infection, it is unclear whether patient counseling should change. The right of future partners to be informed of the risk could also be considered. However, any modification of the provided counseling should be based also on the actual likelihood of a beneficial effect on the incidence of HPV-associated oropharyngeal cancers. The risk is on one side to induce unjustified anxiety and provide ineffective instructions, on the other side to miss the opportunity to limit the spread of oral HPV infections. Thus, major health authorities and international gynecologic scientific societies should issue or update specific recommendations, also with the aim of preventing inconsistent health care professionals’ behaviors.

Regulation Mechanism of Plant Pigments Biosynthesis: Anthocyanins, Carotenoids, and Betalains.

Anthocyanins, carotenoids, and betalains are known as the three major pigments in the plant kingdom. Anthocyanins are flavonoids derived from the phenylpropanoid pathway. They undergo acylation and glycosylation in the cytoplasm to produce anthocyanin derivatives and deposits in the cytoplasm. Anthocyanin biosynthesis is regulated by the MBW (comprised by R2R3-MYB, basic helix-loop-helix (bHLH) and WD40) complex. Carotenoids are fat-soluble terpenoids whose synthetic genes also are regulated by the MBW complex. As precursors for the synthesis of hormones and nutrients, carotenoids are not only synthesized in plants, but also synthesized in some fungi and bacteria, and play an important role in photosynthesis. Betalains are special water-soluble pigments that exist only in Caryophyllaceae plants. Compared to anthocyanins and carotenoids, the synthesis and regulation mechanism of betalains is simpler, starting from tyrosine, and is only regulated by MYB (myeloblastosis). Recently, a considerable amount of novel information has been gathered on the regulation of plant pigment biosynthesis, specifically with respect to aspects. In this review, we summarize the knowledge and current gaps in our understanding with a view of highlighting opportunities for the development of pigment-rich plants.

Blood Pressure Variability and Complexity in Nursing Home Residents Before Death

Blood pressure (BP) is a complex dynamic system in the human body and an important determinant of healthy aging. Exploring BP as a dynamic data system may provide important insights into how BP patterns can provide complementary information to the static, one-time BP measurements that are more commonly used for clinical decision making. Thus, we sought to describe BP as a dynamic data system in older adults nearing death. Using a prospective cohort study design, we assessed BP measures 6 months before death in Veterans Health Administrative nursing home residents between 10/1/2006 and 9/30/2017. Variability was characterized using standard deviation and mean square error after adjusting for diurnal variations. Complexity (i.e., amount of novel information vs. redundancy) was examined using Shannon’s entropy (bits). Generalized linear models were used to examine factors associated with overall BP variability. We identified 17,953 patients (98.0% male, 82.5% White, mean age 80.2 years, and mean BP 125.7/68.6 mmHg). In the last 6 months of life, systolic BP decreased slightly (□-7.2mmHg). Variability was stable until the last month of life, at which point variability increased by as much as 30%. In contrast, complexity did not change in the 6 months before death (□0.02 bits). Factors associated with BP variability before death include hospitalizations, hospice care, and medication changes. Systolic BP decreases in the last 6 months before death, and BP variability increases in the last month of life. Further, the increase in BP variability may be driven by increasingly complex care patterns as one approaches death.

Exploring the Dynamics of Week-to-Week Blood Pressure in Nursing Home Residents Before Death.

Aging is accompanied by an overall dysregulation of many dynamic physiologic processes including those related to blood pressure (BP). While year-to-year BP variability is associated with cardiovascular events and mortality, no studies have examined this trend with more frequent BP assessments. Our study objective is to take the next step to examine week-to-week BP dynamics-pattern, variability, and complexity-before death. Using a retrospective study design, we assessed BP dynamics in the 6 months before death in long-term nursing home residents between 1 October 2006 and 30 September 2017. Variability was characterized using SD and mean squared error after adjusting for diurnal variations. Complexity (i.e., amount of novel information in a trend) was examined using Shannon's entropy (bits). Generalized linear models were used to examine factors associated with overall BP variability. We identified 17,953 nursing home residents (98.0% male, 82.5% White, mean age 80.2 years, and mean BP 125.7/68.6 mm Hg). Despite a slight trend of decreasing systolic week-to-week BP over time (delta = 7.2 mm Hg), week-to-week complexity did not change in the 6 months before death (delta = 0.02 bits). Average weekly BP variability was stable until the last 3-4 weeks of life, at which point variability increased by 30% for both systolic and diastolic BP. Factors associated with BP variability include average weekly systolic/diastolic BP, days in the nursing home, days in the hospital, and changes to antihypertensive medications. Week-to-week BP variability increases substantially in the last month of life, but complexity does not change. Changes in care patterns may drive the increase in BP variability as one approaches death.

WITHDRAWN: Prediction of rumour source identification through spam detection on social Networks- A survey

Online social networks (OSNs) are becoming part of everyone’s virtual world which helps people to connect and disseminate all kind of opinions, news, and other information in real-time. In this era of social networking, an increased amount of novel information being spread with different viewpoints in an unprecedented scale. However, along with useful information propagation, OSNs also serve as fertile land for false information or rumour propagation. This causes irreversible damage to society during emergency events as a negative effect and hinder them from the actual information. Normally, internet media like OSNs, gossip circulate quicker than average. Therefore, studying the rumour dynamics and controlling the rumours in OSNs is of critical research interest in recent times. To achieve this purpose, a robust and reliable centrality model need to be introduced to discover influential spreader by considering the interest of individual’s on neighbor. The opinion of an individual with neighbors is the primary source for this method along with their location in the network. Identifying the rumours during inception stages is difficult due to the lesser availability of data related to the rumour. In this paper a brief survey is done on different rumour source identification techniques.

How long does it take to know a species? Redescription, vocalization, and chigger mites infestation pattern in the centenary emBrachycephalus/em emgarbeanus/em (Anura: Brachycephalidae).

Although we celebrate the centennial of Brachycephalus garbeanus' discovery, little progress has been done on understanding this species' biology apart from a few morphological and ecological studies, which includes its redescription based on three specimens from the type-series, microhabitat use, sexual dimorphism in body size, and feeding habits. This species is endemic to the Serra dos Órgãos Mountain range, in the state of Rio de Janeiro, southeastern Brazil. Here we redescribe B. garbeanus based on a wide sampling, including its advertisement and aggressive calls, and also the chigger mites infestation pattern. The advertisement call is longer than 25.8 s with pulsed notes series emitted at an average rate of 2.3 notes/s and 14.1 pulses/s; long inter-note interval with 320 ms; notes with distinctly short pulses (1 to 16 ms); low dominant frequency for this genus (3.0-5.4 kHz) and presence of four harmonics. This species is often parasitized by chigger mites of Hannemania, with a prevalence of infection of 67%, mainly affecting the ventral body surface. Females had a higher prevalence of parasites than males and there was no correlation found between the size of specimens and the number of parasites. Our study, provides an important and overdue taxonomical contribution, including a large amount of novel information for B. garbeanus.

Multi-Omics Analysis of Small RNA, Transcriptome, and Degradome in T. turgidum-Regulatory Networks of Grain Development and Abiotic Stress Response.

Crop reproduction is highly sensitive to water deficit and heat stress. The molecular networks of stress adaptation and grain development in tetraploid wheat (Triticum turgidum durum) are not well understood. Small RNAs (sRNAs) are important epigenetic regulators connecting the transcriptional and post-transcriptional regulatory networks. This study presents the first multi-omics analysis of the sRNAome, transcriptome, and degradome in T. turgidum developing grains, under single and combined water deficit and heat stress. We identified 690 microRNAs (miRNAs), with 84 being novel, from 118 sRNA libraries. Complete profiles of differentially expressed miRNAs (DEMs) specific to genotypes, stress types, and different reproductive time-points are provided. The first degradome sequencing report for developing durum grains discovered a significant number of new target genes regulated by miRNAs post-transcriptionally. Transcriptome sequencing profiled 53,146 T. turgidum genes, swith differentially expressed genes (DEGs) enriched in functional categories such as nutrient metabolism, cellular differentiation, transport, reproductive development, and hormone transduction pathways. miRNA–mRNA networks that affect grain characteristics such as starch synthesis and protein metabolism were constructed on the basis of integrated analysis of the three omics. This study provides a substantial amount of novel information on the post-transcriptional networks in T. turgidum grains, which will facilitate innovations for breeding programs aiming to improve crop resilience and grain quality.

Autonomous Scene Exploration for Robotics: A Conditional Random View-Sampling and Evaluation Using a Voxel-Sorting Mechanism for Efficient Ray Casting.

Carrying out the task of the exploration of a scene by an autonomous robot entails a set of complex skills, such as the ability to create and update a representation of the scene, the knowledge of the regions of the scene which are yet unexplored, the ability to estimate the most efficient point of view from the perspective of an explorer agent and, finally, the ability to physically move the system to the selected Next Best View (NBV). This paper proposes an autonomous exploration system that makes use of a dual OcTree representation to encode the regions in the scene which are occupied, free, and unknown. The NBV is estimated through a discrete approach that samples and evaluates a set of view hypotheses that are created by a conditioned random process which ensures that the views have some chance of adding novel information to the scene. The algorithm uses ray-casting defined according to the characteristics of the RGB-D sensor, and a mechanism that sorts the voxels to be tested in a way that considerably speeds up the assessment. The sampled view that is estimated to provide the largest amount of novel information is selected, and the system moves to that location, where a new exploration step begins. The exploration session is terminated when there are no more unknown regions in the scene or when those that exist cannot be observed by the system. The experimental setup consisted of a robotic manipulator with an RGB-D sensor assembled on its end-effector, all managed by a Robot Operating System (ROS) based architecture. The manipulator provides movement, while the sensor collects information about the scene. Experimental results span over three test scenarios designed to evaluate the performance of the proposed system. In particular, the exploration performance of the proposed system is compared against that of human subjects. Results show that the proposed approach is able to carry out the exploration of a scene, even when it starts from scratch, building up knowledge as the exploration progresses. Furthermore, in these experiments, the system was able to complete the exploration of the scene in less time when compared to human subjects.

Rediscovery of the toadlet Brachycephalus bufonoides Miranda-Ribeiro, 1920 (Anura: Brachycephalidae) with osteological and acoustic descriptions.

Brachycephalus bufonoides was described as a "variety" of B. ephippium based on two specimens which 90 years later was considered full species. Besides its brief original description, nothing else is known for this species. Herein we report the rediscovery of the pumpkin-toadlet Brachycephalus bufonoides from Nova Friburgo, State of Rio de Janeiro, the second most populous area within the Atlantic Forest in Brazil. A detailed osteological description of this species was also provided, including skull, hyolaryngeal skeleton and postcranium skeleton. The laryngeal skeleton of Brachycephalus genus was depicted for the first time. We conducted a molecular phylogenetic analysis of Brachycephalus using DNA sequences comprising two fragments of mitochondrial gene (16S). Both analysis with Bayesian inference and maximum parsimony supported the recognition of B. bufonoides as an exclusive lineage, allocated within the B. ephippium species group in B. vertebralis lineage. We improved the diagnosis and variation of the species, including more collected specimens, coloration in vivo and advertisement call description. Compared with its congeners, B. bufonoides has skin on head and dorsum with dermal hyperossification; skull with hyperossification of postorbital crests; a pair of hyperossified bulges about equidistant between postorbital crests; fourth presacral vertebra with transverse process hyperossified, ornamented and sacral diapophyses hyperossified, which can be seen externally (lineage of B. vertebralis sensu Condez et al. 2020); presence of dermal ossification as separated bulges of each vertebrae; general background color orange with different intensities of dark orange blotches on dorsum, including bordering of sacral region; absence of osteoderms and presence of warts on the dorsolateral surface of body; medium body size (SVL of adults: 12.0-14.5 mm for males and 14.7-16.3 mm for females; Table 1); rough dorsum; advertisement calls with 13 to 17 pulses; presence of pulse period modulation; and advertisement calls with notes longer than 0.2 s (0.22 to 0.31 s). Herein an important contribution for the taxonomy and systematics of this genus is provided, including a large amount of novel information for B. bufonoides from different sources (i.e., molecular, morphological variation, bioacoustic), allowing it to be included in future studies of species delimitation and relationships within Brachycephalus. Also, the discovery of this species reiterates the importance of Nova Friburgo for the conservation of the Atlantic Forest biodiversity.

Between Innovation and Standardization, Is There Still a Room for Scientific Reports? The Rise of a Formatting Tradition in Periodontal Research

Everybody, regardless of their role, is aware that biomedical research is rapidly evolving, and the demand for reproducibility is increasing together with the amount of novel information. “Before reproducibility must come pre-producibility” “Checklists work to improve science”, just to quote some of the articles querying how to find a new bridge between ethics in science and the urgency for publishing. Looking for papers on anti-inflammatory compounds in periodontics, we came across a significant number of articles that could be considered a prototype of a consistent study format. The literature on the testing of active compounds on lipopolysaccharides- (LPS)-induced inflammation in gingival fibroblasts was searched to identify studies that followed a consistent format, to better understand their similarities and assess the appropriateness of their methods. Several studies were identified with a degree of similarity in their methods and formatting that was so high that it was possible to rule out that it was due to chance, and a format template common to these studies was outlined. Although this was most likely beyond the intentions of their authors, these studies may pose the basis for an in-vitro testing standard for anti-inflammatory compounds; however, the dangers of acritical uniformity are also apparent.

A Comprehensive Proteomic Analysis of Metastatic Cancer Progression in a Murine Model of Tumorigenesis Using Orbitrap Tandem Mass Spectrometry

While the subject of cancer metastasis has been extensively studied, research is typically limited to a few target proteins or signaling pathways analyzed via traditional means (Western blot, ELISA, etc.). In this study we have utilized cutting edge proteomic analysis to produce a database of protein expression data across seven serially passaged mouse cell lines to explore the progression of metastasis. We hypothesized that Orbitrap tandem mass spectrometry (OTMS) would replicate association of specific proteins to metastasis and delineate previously unidentified proteins. These cell lines included the parental embryonic fibroblasts (NIH/3T3), 3T3 cells transformed with a human oncogene, T1‐A cells from a primary tumor, T2‐A cells from a slightly metastatic tumor, and T3‐PA, T3‐HA, and T4‐PA cells derived from lung and liver metastases. OTMS yielded expression data for over 3,800 proteins across all seven cell lines. These outputs were considered to reach a threshold of change if protein expression was up‐or downregulated by at least two‐fold as compared to NIH/3T3 cells. There were 50 proteins that reached threshold in all six cancerous cell lines. Of these, 12% are directly associated with cell death and 18% with differentiation. Fourteen proteins reached threshold in only the metastatic cell lines. Correlation of protein up‐ or downregulation across these lines may assist in the elucidation or delineation of signal cascades and checkpoints that result in metastasis. Indeed, some of these proteins have been identified as tumorigenic via traditional means, such as moesin, which promotes invasion and metastasis, and PIN1, which positively correlates with proliferation and anchorage independent growth. Interestingly, 152 proteins met threshold expression only in T2‐A cells while being expressed at subthreshold levels in both non‐metastatic cells and highly metastatic cells, which is over twice the number of threshold proteins that were uniquely expressed in any single cell line. As T2‐A was the first cell line isolated from a metastatic growth, these data are evidence that these proteins are necessary for the conversion of a cell from a localized tumor to a metastatic cancer cell, but which return to normalized expression levels after this initial conversion. This study has yielded an impressive proteomic database to offer insight into the development of metastasis in embryonic mouse fibroblasts. It should provide a valuable resource to researchers examining how particular proteins of interest may act within the larger context of metastatic conversion. Furthermore it validates the efficacy of OTMS proteomic analysis by identifying proteins which had previously been recognized as tumorigenic via traditional proteomic studies, while gaining an unprecedented amount of novel information on models of cancer development.Support or Funding InformationThis research was supported by a grant from Oleander Medical Technologies.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

Virus⁻Host Interactions Involved in Lassa Virus Entry and Genome Replication.

Lassa virus (LASV) is the causative agent of Lassa fever, a human hemorrhagic disease associated with high mortality and morbidity rates, particularly prevalent in West Africa. Over the past few years, a significant amount of novel information has been provided on cellular factors that are determinant elements playing a role in arenavirus multiplication. In this review, we focus on host proteins that intersect with the initial steps of the LASV replication cycle: virus entry and genome replication. A better understanding of relevant virus–host interactions essential for sustaining these critical steps may help to identify possible targets for the rational design of novel therapeutic approaches against LASV and other arenaviruses that cause severe human disease.

The effects of genomic germline variant reclassification on clinical cancer care.

The last two decades have provided an astounding amount of novel information about the human genome. Translating germline genomic data into clinically actionable findings is reliant on the annotation and laboratory classification of specific variants. Variant classification helps providers and patients determine if genomic findings can inform clinical management. In germline hereditary cancer predisposition testing, variants of uncertain significance (VUS) are routinely misunderstood. By definition, they cannot be classified by the testing laboratory as either problematic mutations or benign variants. Many VUS undergo category reclassifications over time (from months to years after initial classification) as more information is known about normal human genomic diversity, especially among underrepresented minority populations. When VUS are reclassified, it has been shown that they are often downgraded. Likewise, some variants originally thought to be actionable mutations are downgraded to VUS or benign variants. Rarely but importantly, VUS may be reclassified in a manner that increases their initial clinical significance. Here, we discuss the insights gained from the study of variant reclassification. We provide a case series to highlight the potential impact that variant reclassifications can have on individual and family cancer management, risk counseling, and screening.

Genome-Wide Comparative Analysis of Aspergillus fumigatus Strains: The Reference Genome as a Matter of Concern.

Aspergillus fumigatus is a ubiquitous saprophytic mold and a major pathogen in immunocompromised patients. The effectiveness of triazole compounds, the A. fumigatus first line treatment, is being threatened by a rapid and global emergence of azole resistance. Whole genome sequencing (WGS) has emerged as an invaluable tool for the analysis of genetic differences between A. fumigatus strains, their genetic background, and antifungal resistance development. Although WGS analyses can provide a valuable amount of novel information, there are some limitations that should be considered. These analyses, based on genome-wide comparative data and single nucleotide variant (SNV) calling, are dependent on the quality of sequencing, assembling, the variant calling criteria, as well as on the suitable selection of the reference genome, which must be genetically close to the genomes included in the analysis. In this study, 28 A. fumigatus genomes sequenced in-house and 73 available in public data bases have been analyzed. All genomes were distributed in four clusters and showed a variable number of SNVs depending on the genome used as reference (Af293 or A1163). Each reference genome belonged to a different cluster. The results highlighted the importance of choosing the most suitable A. fumigatus reference genome to avoid misleading conclusions.

Using information theory to identify redundancy in common laboratory tests in the intensive care unit.

BackgroundClinical workflow is infused with large quantities of data, particularly in areas with enhanced monitoring such as the Intensive Care Unit (ICU). Information theory can quantify the expected amounts of total and redundant information contained in a given clinical data type, and as such has the potential to inform clinicians on how to manage the vast volumes of data they are required to analyze in their daily practice. The objective of this proof-of-concept study was to quantify the amounts of redundant information associated with common ICU lab tests.MethodsWe analyzed the information content of 11 laboratory test results from 29,149 adult ICU admissions in the MIMIC II database. Information theory was applied to quantify the expected amount of redundant information both between lab values from the same ICU day, and between consecutive ICU days.ResultsMost lab values showed a decreasing trend over time in the expected amount of novel information they contained. Platelet, blood urea nitrogen (BUN), and creatinine measurements exhibited the most amount of redundant information on days 2 and 3 compared to the previous day. The creatinine-BUN and sodium-chloride pairs had the most redundancy.ConclusionsInformation theory can help identify and discourage unnecessary testing and bloodwork, and can in general be a useful data analytic technique for many medical specialties that deal with information overload.

A Review of the Geographical Distribution and Habitat of the Atlantic Humpback Dolphin (Sousa teuszii).

Understanding of the distributional ecology of the Atlantic humpback dolphin (Sousa teuszii) has been hampered by a lack of systematic and consistent sampling effort. The only comprehensive species distribution review was published in 2004; since then a considerable amount of novel information has emerged. We compiled 853 sighting, capture and specimen records of the species, and produced global and regional distribution maps. Of the 830 records where year was available, 63.1% dated from ≥2005 and confirm a contemporary occurrence in six marine ecoregions and 11 countries: Western Sahara, Mauritania, Senegal, Gambia, Guinea-Bissau, Guinea, Benin, Cameroon, Gabon, Congo Republic and Angola. Additionally, Togo is a recently confirmed range state. Group sizes ranged from 1 to 45 animals, with small groups of 1 to 10 animals comprising 65% of the sightings. Similarities were noted in the regions inhabited by Atlantic humpback dolphins across their range, particularly an occurrence in relatively shallow (predominantly ≤20 m) depths, in warm waters (average SSTs of 15.8-31.8°C) and in dynamic habitat strongly influenced by tidal patterns. These conditions occur in various habitats occupied by the species, including estuarine systems, open coasts, archipelagos, tidal mud-flats and sheltered bays. Sightings were recorded at distances of 13 m to 12.8 km (mean of 573 m) from land, indicating that the species occurs several kilometres from shore when suitable shallow habitat is present. The Atlantic humpback dolphin may be a 'nearshore' species based on oceanographic definitions incorporating water depth, wave action and sedimentation rather than on spatial distance from the coast.

Research progress in quantifying the mechanical properties of single living cells using atomic force microscopy

The advent of atomic force microscopy (AFM) provides a powerful tool for investigating the behaviors of single living cells under near physiological conditions. Besides acquiring the images of cellular ultra-microstructures with nanometer resolution, the most remarkable advances are achieved on the use of AFM indenting technique to quantify the mechanical properties of single living cells. By indenting single living cells with AFM tip, we can obtain the mechanical properties of cells and monitor their dynamic changes during the biological processes (e.g., after the stimulation of drugs). AFM indentation-based mechanical analysis of single cells provides a novel approach to characterize the behaviors of cells from the perspective of biomechanics, considerably complementing the traditional biological experimental methods. Now, AFM indentation technique has been widely used in the life sciences, yielding a large amount of novel information that is meaningful to our understanding of the underlying mechanisms that govern the cellular biological functions. Here, based on the authors' own researches on AFM measurement of cellular mechanical properties, the principle and method of AFM indentation technique was presented, the recent progress of measuring the cellular mechanical properties using AFM was summarized, and the challenges of AFM single-cell nanomechanical analysis were discussed.

Genetics of the First Seven Proprotein Convertase Enzymes in Health and Disease

Members of the substilisin/kexin like proprotein convertase (PCSK) protease family cleave and convert immature pro-proteins into their biologically active forms. By cleaving for example prohormones, cytokines and cell membrane proteins, PCSKs participate in maintaining the homeostasis in a healthy human body. Conversely, erratic enzymatic function is thought to contribute to the pathogenesis of a wide variety of diseases, including obesity and hypercholestrolemia. The first characterized seven PCSK enzymes (PCSK1-2, FURIN, PCSK4-7) process their substrates at a motif made up of paired basic amino acid residues. This feature results in a variable degree of biochemical redundancy in vitro, and consequently, shared substrate molecules between the different PCSK enzymes. This redundancy has confounded our understanding of the specific biological functions of PCSKs. The physiological roles of these enzymes have been best illustrated by the phenotypes of genetically engineered mice and patients that carry mutations in the PCSK genes. Recent developments in genome-wide methodology have generated a large amount of novel information on the genetics of the first seven proprotein convertases. In this review we summarize the reported genetic alterations and their associated phenotypes.

Swiftdiscovery of the orbital period of the high mass X-ray binary IGR J015712−7259 in the Small Magellanic Cloud

In the last years the hard X-ray astronomy has made a significant step forward, thanks to the monitoring of the IBIS/ISGRI telescope on board the INTEGRAL satellite and of the Burst Alert Telescope (BAT) on board of the Swift observatory. This has provided a huge amount of novel information on many classes of sources. We have been exploiting the BAT survey data to study the variability and the spectral properties of the new high mass X-ray binary sources detected by INTEGRAL. In this letter we investigate the properties of IGR J015712-7259. We perform timing analysis on the 88-month BAT survey data and on the XRT pointed observations of this source. We also report on the broad-band 0.2-150 keV spectral analysis. We find evidence for a modulation of the hard-X-ray emission with period P_o=35.6 days. The significance of this modulation is 6.1 standard deviations. The broad band spectrum is modeled with an absorbed power law with photon index Gamma 0.4 and a steepening in the BAT energy range modeled with a cutoff at an energy of ~13 keV.}