Ammonia Release Research Articles

The extent of nitrogen isotopic fractionation in rumen bacteria is associated with changes in rumen nitrogen metabolism.

Nitrogen use efficiency is an important index in ruminants and can be indirectly evaluated through the N isotopic discrimination between the animal and its diet (Δ15Nanimal-diet). The concentration and source of N may determine both the extent of the N isotopic discrimination in bacteria and N use efficiency. We hypothesised that the uptake and release of ammonia by rumen bacteria will affect the natural 15N enrichment of the bacterial biomass over their substrates (Δ15Nbacteria-substrate) and thereby further impacting Δ15Nanimal-diet. To test this hypothesis, two independent in vitro experiments were conducted using two contrasting N sources (organic vs inorganic) at different levels either in pure rumen bacteria culture incubations (Experiment #1) or in mixed rumen cultures (Experiment #2). In Experiment #1, tryptone casein or ammonium chloride were tested at low (1 mM N) and high (11.5 mM N) concentrations on three rumen bacterial strains (Fibrobacter succinogenes, Eubacterium limosum and Xylanibacter ruminicola) incubated in triplicate in anaerobic batch monocultures during 48h. In Experiment #2 mixed rumen cultures were incubated during 120 h with peptone or ammonium chloride at five different levels of N (1.5, 3, 4.5, 6 and 12-mM). In experiment #1, Δ15Nbacteria-substrate was lowest when the ammonia-consumer bacterium Fibrobacter succinogenes was grown on ammonium chloride, and highest when the proteolytic bacterial strain Xylanibacter ruminicola was grown on tryptone. In experiment #2, Δ15Nbacteria-substrate was lower with inorganic (ammonium chloride) vs organic (peptone) N source. A strong negative correlation between Δ15Nbacteria-substrate and Rikenellaceae_RC9_gut_group, a potential fibrolytic rumen bacterium, was detected. Together, our results showed that Δ15Nbacteria-substrate may change according to the balance between synthesis of microbial protein from ammonia versus non-ammonia N sources and confirm the key role of rumen bacteria as modulators of Δ15Nanimal-diet.

Insights into effects of thermotolerant nitrifying and sulfur-oxidizing inoculants on nitrogen-sulfur co-metabolism in sewage sludge composting

In this study, high temperature thermotolerant nitrifying bacteria (TNB) and high temperature thermotolerant sulfide oxidizing bacteria (TSOB) were obtained from compost samples and inoculated into sewage sludge (SS) compost. The effects of inoculation on physical and chemical parameters, ammonia and hydrogen sulfide release, nitrogen form and sulfur compound content change and physical-chemical properties during nitrogen and sulfur conversion were studied. The results showed that inoculation of TNB and TSOB increased the temperature, pH, OM degradation, C/N ratio and germination index (GI) of compost. Compared with the control treatment (CK), the addition of inoculants reduced the release of NH3 and H2S, and transformed them into nitrogen and sulfur compounds, the hydrolysis of polymeric ferrous sulfate was promoted, resulting in relatively high content of sulfite and sulfate. At the same time, the physical and chemical properties of SS have a strong correlation with nitrogen and sulfur compounds.

Preparation, Features, and Efficiency of Nanocomposite Fertilisers Based on Glauconite and Ammonium Dihydrogen Phosphate.

This paper studies the chemical and mechanochemical preparation of glauconite with ammonium dihydrogen phosphate (ADP) nanocomposites with a ratio of 9:1 in the vol.% and wt.%, respectively. The methods include X-ray diffraction analysis, scanning electron microscope with energy-dispersive X-ray spectroscopy, transmission electron microscopy, infrared spectroscopy, and differential thermal analysis with a quadruple mass spectrometer. The manufactured nanocomposites keep the flaky glauconite structure. Some glauconite unit structures have been thickened due to minimal nitrogen (ammonium) intercalation into the interlayer space. The globular, granular, or pellet mineral particles of nanocomposites can be preserved via chemical techniques. Globular and micro-aggregate particles in nanocomposites comprise a thin film of adsorbed ADP. The two-step mechanochemical method makes it possible to slightly increase the proportion of adsorbed (up to 3.2%) and intercalated (up to 6.0%) nutrients versus chemical ways. Nanocomposites prepared via chemical methods consist of glauconite (90%), adsorbed (1.8-3.6%), and intercalated (3.0-3.7%) substances of ADP. Through the use of a potassium-containing clay mineral as an inhibitor, nitrogen, phosphorus, and potassium (NPK), nanocomposite fertilisers of controlled action were obtained. Targeted and controlled release of nutrients such as phosphate, ammonium, and potassium are expected due to various forms of nutrients on the surface, in the micropores, and in the interlayer space of glauconite. This is confirmed via the stepwise dynamics of the release of ammonium, nitrate, potassium, and phosphate from their created nanocomposites. These features of nanocomposites contribute to the stimulation of plant growth and development when fertilisers are applied to the soil.

Short flow and green process for the production of V2O3 powder via ammonia reduction method

BackgroundThe NH3, a clean reductant, generated by the thermolysis of NH4VO3, is not effectively utilized in the conventional process of vanadium metallurgy. Additionally, the preparation process of V2O3 is both long and costly. Ammonia recovery is essential to prevent environmental issues resulting from ammonia release. By direct thermolysis of NH4VO3 and effectively utilizing of active NH3, it is possible to achieve the short flow and green process for the production of V2O3. MethodIn an open NH3 atmosphere, one end of the flange was connected to the liquid ammonia gas cylinder, while the other end was connected to the tail gas absorber. Furthermore, in a closed NH3 atmosphere, both ends of the tube furnace were sealed with flanges, and the initial atmosphere in the tube was pre-injection NH3. The products of NH4VO3 thermolysis were characterized using various techniques, including XRD, FT-IR, ICP-OES, SEM, XPS, and TG. Significant findingThe thermolysis process of NH4VO3 with pre-injection of NH3 under the sealed condition was a simulation of the continuous production process of V2O3 using a rotary kiln in industrial production. The rational utilization of ammonia resulted in the reduce of energy consumption, extra reductant consumption, and significant NH3 emissions compared to the traditional V2O3 production process. It was observed that V2O3 was easily obtained through the thermolysis of NH4VO3 with pre-injection of NH3 under the sealed condition at 600 °C for 3 h, using a dosage of NH4VO3 ranging from 2 g to 10 g. During this process, the NH4VO3 went through the phase transition as follows: NH4VO3→NH4V3O8→(NH4)0.38V2O5→V6O13→VO2→V4O7→V3O5→V2O3, and the relevant characterization of products showed that the V2O3 obtained was qualified. These experimental results were of great significance to the industrial practice of vanadium metallurgy. Based on these results, short flow and green process of V2O3 could be achieved in industry by partially improving the calcination process of NH4VO3 in large-scale rotary kilns.

MurKy waters: Modeling the succession from r to K strategists (diatoms to dinoflagellates) following a nutrient release from a mining facility in Florida

AbstractThe impacts of pulsed nutrient injections or extreme runoff events on marine ecosystems are far less studied than those associated with long‐term eutrophication, particularly in regard to mechanisms regulating the response of plankton community structure. Over 800 million liters of nutrient‐rich water from a fertilizer mine were discharged over a 2‐week period into Tampa Bay, Florida, in 2021, providing a unique opportunity to document the plankton response. A 3D‐coupled hydrodynamic biogeochemical model was developed to investigate this response and to understand the observed succession of a large, short diatom bloom followed by a secondary Karenia brevis bloom that lasted through the summer. The model reproduced the observed changes in nutrient concentration, total chlorophyll a, and diatom and K. brevis biomass in Tampa Bay. With a faster growth rate and spring temperature close to the optimal window of growth, diatoms had an initial competitive advantage, with 2/3 of the nutrient uptake due to ammonium and 1/3 due to nitrate. However, exhaustion of external nutrients led to the rapid decline of the diatom bloom, and the associated particular organic nitrogen sank onto the bay sediment. Enhanced sediment release of ammonium during the weeks following, and summer remineralization of dissolved organic nitrogen provided sufficient regenerated nitrogen to support slow‐growing K. brevis that could capitalize on low nutrient conditions. Modeling analysis largely confirmed Margalef's conceptual model of r to K‐selected species succession and provided additional insights into nutrient cycling supporting the initial diatom bloom and the subsequent bloom of a slow‐growing harmful algal species.

Cyanobacterial water blooms as an environmental hazard to waters

The article describes and analyzes the problem of cyanotoxin contamination of water bodies in Ukraine and other countries of the world. Eutrophication of water bodies, which is caused by human activity, climate change lead to uncontrolled development of cyanobacteria, and thus to ecological danger in hydrosphere objects. Anthropogenic eutrophication (increasing the content of nutrients in water due to their introduction into water bodies during pollution of water bodies by flood and municipal runoff, fish farming use of water bodies), temperature rise, increase in carbon dioxide content in the atmosphere, as a result of the transformation of introduced organic matter in water, contribute to increasing the dominance of cyanobacteria in water ecosystems. Bloomforming cyanobacteria produce various neurotoxins, hepatotoxins, and dermatotoxins that can be fatal to birds and mammals (including waterfowl, cattle, and dogs) and threaten the use of water bodies for public water supply, recreation, irrigation, and fishing. Among the toxins of blue-green algae, nodularin, saxitoxin, microcystin, domoic acid, guanitoxin, anatoxins and others were found. Exceeding the critical mass of algae and their metabolites activates the selfdecomposition process. This leads to the removal of oxygen from the water, and instead the release of methane, hydrogen sulfide, ammonia, and other toxic substances. The result of this is not only the death of fish. Cyanobacteria are capable of natural genetic transformation — genetic change of the cell as a result of direct absorption and inclusion of exogenous DNA from its environment. A sufficiently effective solution to the issue of controlling the activity of cyanobacteria is the use of humic preparations. it was established that humic substances (humic acids, fulvic acids) in certain concentrations negatively affect the growth, development and activity of cyanobacteria. Humic substances, as a component of organic matter and a reserve of organic carbon in its global cycle, have a negative effect on hydrobionts by increasing their concentration in water bodies. However, humic substances are able to regulate the activity of phytoplankton. The introduction of humic substances at a concentration of 2-5 mg/l of reservoir water significantly affects the activity of phytoplankton (suppresses phytoplankton vegetation, reduces the slime formation of cyanobacteria, and, from the beginning, inactivates the synthesis of cyanotoxins). This effect is provided by the alkaline pH of humates, high content of iron and copper.

Impact of Varying Mass Concentrations of Ammonia Nitrogen on Biogas Production and System Stability of Anaerobic Fermentation

High ammonium release from chicken manure poses a significant limitation to aerobic digestion, impeding microbial processes and inhibiting biogas production. In this study, we conducted anaerobic digestion of a mixture consisting of chicken manure and corn straw as the fermented raw material. The inoculum used was obtained from the residue of previously fermented chicken manure. To assess the inhibitory effect, we varied the ammonia levels within the range of 750–4250 mg/L by introducing ammonium chloride. The efficiency of aerobic digestion was monitored through the measurement of volatile fatty acids (VFA), chemical oxygen demand (COD), total inorganic carbon (TOC), and methane yield. Our results indicated that elevated levels of ammonia nitrogen had a suppressive impact on methane release, and this decrease followed a linear relationship with the increasing ammonia nitrogen load. Moreover, the addition of ammonia led to a slower release, with the maximum daily ammonia concentration observed at 15 days compared to the 6th day at lower ammonia levels. Furthermore, on the 40th day of aerobic digestion, the cumulative methane production at 4250 mg/L was inhibited by 41% compared to the 750 mg/L condition. The patterns of VFA, inorganic carbon, and COD reduction were consistent across all ammonia levels, with VFA and TOC levels being highest at the highest ammonia concentration and lowest at the lowest ammonia concentration. The accumulation of VFA resulted in a decrease in pH and a decline in methanogenic activity. Additionally, high ammonia levels altered the relative abundance of methanogens. Acetoclastic methanogens (Methanosaeta) exhibited a decrease in abundance, while hydrogenotrophic methanogens (Methanosaeta, Methanoculleus) and methylotrophic methanogens (Candidatus Methanoplasma) demonstrated an increase in abundance. Overall, our findings highlight the inhibitory effects of high ammonia concentrations on biogas production, providing insights into the changes in microbial composition and activity during anaerobic fermentation.

Valorization of potato starch wastewater using anaerobic acidification coupled with Chlorella sorokiniana cultivation

Wastewater from the potato processing industry called protamylasse is rich in proteins and carbohydrates that potentially can be valorized through cultivation of microalgae by mixotrophic metabolism. However, the complex organic compounds are a challenge, as algae grow best on simple compounds such as volatile fatty acids (VFA). This study demonstrates a new two-stage system. First, VFA production was achieved by testing mesophilic and thermophilic anaerobic acidification (AA) at a short hydraulic retention time (HRT; 3.3 and 5 days) resulting in the release of ammonium and phosphate. HRT of 5 days and thermophilic conditions was optimal considering the high acetate yield of 0.23 g and 22 ml CH4 per g volatile solids (VS). Then, Chlorella sorokiniana was chosen based on the obtained growth rate, and better adaption in ammonium-rich AA effluent after screening several tested microalgae (Chlorella sorokiniana, Chlorella vulgaris, Scenedesmus obliquus, and Haematococcus pluvialis). It was cultivated for valorization of nutrients and organics and successfully upscaled to 25 L photobioreactor (PBR) scale under both batch and continuous operation with high dosage of 25% (8.2 g L−1 of VS) of AA effluent at an HRT of 5 days in the PBR. Chlorella sorokiniana removed more than 99% of the chemical oxygen demand (COD) and the VFA during continuous flow PBR operation. This approach contributed to the final removal efficiency of 71%, 91%, and 78% for phosphorus, nitrate, and ammonia, respectively, and production of microalgae biomass with more than 73% protein. Thus, a promising process for simultaneous treatment of high strength wastewater for microalgal protein production.

Effective abatement of ammonium and nitrate release from sediments by biochar coverage

Inorganic forms of N from sediments and runoff water, among others, remain some of the key sources of pollution of water bodies. However, the release of NH4+-N from sediment to water can be effectively reduced by biochar coverage due to high adsorption capacity, unlike NO3—N, where biochar has a low affinity. The feasibility of biochar coverage to abate NO3−-N release needs to be evaluated. This study collected four sediments from Lake Taihu (China). Three types of biochar pyrolyzed from ordinary wastes, coconut shell (coBC), algal and excess sludge, were prepared to cover them and were incubated for 90 days. Results showed that the terminal total nitrogen (TN) and NO3−-N concentrations decreased from 5.35 to 2.31–3.04 mg/L, 3.05 to 0.34–1.11 mg/L, respectively. CoBC coverage showed the best performance for reducing NO3−-N release flux from 26.99 ± 0.19 to 9.30 ± 0.02 mg/m2·d (63.6 %). Potential denitrifiers, such as Flavobacterium and Exiguobacterium, were enriched in the biochar-coverage layer, and the absolute abundance of N-related functional genes (narG, nirS, nosZ and anammox) was increased by 1.76–4.21 times (p < 0.05). Jar tests by 15N isotope labeling further indicated that biochar addition increased the denitrification and anammox rates by 53.5–83.4 %. Experiments combining exogenous organic‑carbon addition and 15N labeling demonstrated that biochar's key role was regulating organic matter's bioavailability. Analysis with partial least square path modeling (PLS-PM) implied biochar with higher adsorption enhanced the denitrification and anammox processes in sediments via modifying the niche with suitable DOC, TN, and pH. This study suggested that biochar coverage could effectively abate NO3−-N release from sediments by affecting the denitrification and anammox processes.

Study of the key biotic and abiotic parameters influencing ammonium removal from wastewaters by Fe3+-mediated anaerobic ammonium oxidation (Feammox)

The release of ammonia (as NH4+) into water bodies causes serious environmental problems. Therefore, the removal of ammonia from wastewater effluents has become a worldwide concern. New autotrophic biological alternatives for ammonia removal could reduce the limitations of conventional organic carbon-dependent nitrification-denitrification methods. Here, the potential of anaerobic ammonium oxidation coupled to Fe3+ reduction (a process known as Feammox) is studied in wastewater treatment plants of the yeast and beer production industry, not related to ammonium or iron treatment. This process is presented as a viable option to improve the efficiency of ammonia removal from wastewater. The results of this study show that enrichments under Feammox conditions achieved removals of 28.19–32.25% of the total NH4+. The highest rates of ammonium removal and Fe3+ reduction were achieved using FeCl3 as iron source and pH = 7.0. Different environmental conditions for the enrichments were studied and it was found that the use of sodium acetate as a carbon source and an incubation temperature of 35 °C presented higher rates of iron reduction and higher increase in nitrate concentration, related to ammonium oxidative processes. Likewise, the presence of relevant species of the iron and nitrogen cycles as Ferrovum myxofaciens, Geobacter spp, Shewanella spp, Albidiferax ferrireducens and Anammox was verified, supporting the findings of this study. These results provide information that may be relevant to the potential applicability of Feammox to treat wastewater with high ammonia load and could help develop cost-effective and environmentally friendly methods for ammonium removal in wastewater treatment plants.

Releasing mechanism of ammonium during clayey sediments compaction and its impact on groundwater environment

Compaction of clayey aquitard would release pore water containing high levels of ammonium to adjacent aquifers, potentially affecting the concentration of groundwater ammonium. However, the releasing process and impact effect of ammonium within clayey aquitard during compaction remain unknown. Four groups of pre-experiments and two groups of simulation experiments were conducted to reveal the releasing mechanism of ammonium during clayey sediment compaction. (1) The results of Experiment A simulating continuous sedimentation conditions showed that the sediment ammonium transferred into pore water sequentially through desorption of ion exchange form, degradation of organic matter, and simultaneous release of mineral-bound ammonium. The concentration of pore water ammonium was 3.54–8.20 mg N/L, with a significant periodical variation due to sediment ammonium transformation. The lower moisture content (<42.4 %) in the later stage of compaction inhibited the biological transformation of ammonium, and the change in mineral structure caused the isomorphic replacement of K to capture ammonium, resulting in a decrease in ammonium concentration in released pore water. (2) The results of Experiment B simulating artificial compaction conditions (such as land subsidence) showed that the pore water ammonium was primarily caused by desorption of ion exchange form ammonium due to changes in pore structure and moisture content. The ammonium concentration in pore water was 4.72–9.91 mg N/L, with a significant increase in response to a large change in pressure in the short term. (3) The estimate results in the Chen Lake wetland suggested that the contribution of clayey aquitard compaction to groundwater ammonium concentration in the adjacent aquifer would be 2.68–4.29 mg N/L, which accounted for a considerable portion of groundwater ammonium concentration and was far higher than that of advection and diffusion. The findings of this study reveal the releasing mechanism of ammonium during clayey sediments compaction, in which reaction products may affect adjacent aquifers.

Impact of reservoir operation policies on nitrogen cycling processes and water quality dynamics in a large water supply reservoir

Reservoir operation policies affect reservoir nitrogen (N) cycling and budgets through complex influences on physical and biogeochemical processes in water and sediment. However, the underlying influencing mechanisms, especially the impact of operations on sediment processes, remain unclear. This study combines a coupled hydrodynamic-eutrophication-sediment model and a reservoir operation model to clarify reservoir operation impact on N cycling processes in water and sediment as well as impact on stored and discharged water quality. Three typical reservoir operation policies (i.e., standard, hedging, and eco-friendly) were selected to create operation scenarios. The hydrodynamic-eutrophication-sediment model was used to simulate N cycling processes and N concentrations in stored and discharged water under each scenario. Using China's Danjiangkou Reservoir as a case study, results revealed that reservoir operation policies significantly affected spatiotemporal dynamics of N cycling processes and water quality. Specifically, reservoir sediment acted as a N source, contributing to annual net release of 9.5–16.1 Gg N. Higher water levels promoted sediment ammonium release through effects on reservoir submerged area and sediment temperature. Moreover, denitrification processes in water and sediment collectively contributed to annual N removal of 14.0–16.9 Gg N. Annual N removal was greater in the higher water level scenario despite its lower denitrification rate per unit area. Additionally, these processes and hydrodynamic conditions combine to affect N forms and concentrations. The N concentration in the lower water level scenario was generally higher, risking algal bloom outbreaks in the shallow-water zone. This study is intended to help guide reservoir operation policymaking to account for N management while also coordinating water quantity and quality targets.

Casein as protein and hydrolysate: Biostimulant or nitrogen source for Nicotiana tabacum plants grown in vitro?

In contrast to inorganic nitrogen (N) assimilation, the role of organic N forms, such as proteins and peptides, as sources of N and their impact on plant metabolism remains unclear. Simultaneously, organic biostimulants are used as priming agents to improve plant defense response. Here, we analysed the metabolic response of tobacco plants grown in vitro with casein hydrolysate or protein. As the sole source of N, casein hydrolysate enabled tobacco growth, while protein casein was used only to a limited extent. Free amino acids were detected in the roots of tobacco plants grown with protein casein but not in the plants grown with no source of N. Combining hydrolysate with inorganic N had beneficial effects on growth, root N uptake and protein content. The metabolism of casein-supplemented plants shifted to aromatic (Trp), branched-chain (Ile, Leu, Val) and basic (Arg, His, Lys) amino acids, suggesting their preferential uptake and/or alterations in their metabolic pathways. Complementarily, proteomic analysis of tobacco roots identified peptidase C1A and peptidase S10 families as potential key players in casein degradation and response to N starvation. Moreover, amidases were significantly upregulated, most likely for their role in ammonia release and impact on auxin synthesis. In phytohormonal analysis, both forms of casein influenced phenylacetic acid and cytokinin contents, suggesting a root system response to scarce N availability. In turn, metabolomics highlighted the stimulation of some plant defense mechanisms under such growth conditions, that is, the high concentrations of secondary metabolites (e.g., ferulic acid) and heat shock proteins.

Formulasi dan Evaluasi Tablet Urea Lepas Lambat Berlapis Biopolimer Poliasam Laktat Sebagai Suplemen Ruminansia

Urea is a source of non-protein nitrogen (NPN) which can be used to replace pure protein in ruminants. Urea is easily soluble and is degraded to ammonia by rumen bacteria, thus affecting the quality of ruminants. To increase the utilization of urea as a supplement is to try to slow its release into ammonia in the rumen so that it can be used efficiently by rumen microbes to form microbial protein. Slow release urea tablets as an alternative can control the rate of urea degradation and release of ammonia into the rumen and increase rumen efficiency. This study aims to determine the rate and pattern of release of urea tablets in the rumen so that their use is more practical and the dosage is correct. To maintain ammonia levels in the rumen, an alternative polymer-coated urea slow release tablet was designed. Based on this, it was carried out to manufacture slow release urea tablets with biopolymer polylactic acid (PLA) coating in the rumen fluid of ruminants. Furthermore, an in vitro evaluation was carried out by measuring pH into rumen fluid and measuring NH3 by UV-Vis spectro analysis at a wavelength of 630 nm. The results of the test for urea release in the rumen in formulas 1, 2 and 3 were 75.133%, 76.076% and 74.051% respectively, while the release of urea in uncoated urea tablets was 96.384%, these results indicate that tablets with lactic acid coated polyacid last longer. release compared to urea tablets without biopolymer coating.

Surface engineering of Al2O3 nanotubes by ureasolysis method for activating persulfate degradation of antibiotics

Though ecofriendly, pure Al2O3 has never been used for activation of peroxodisulfate (PDS) to degrade pollutants. We report the fabrication of Al2O3 nanotubes by ureasolysis method for efficient activating PDS degradation of antibiotics. The fast ureasolysis in aqueous AlCl3 solution produces NH4Al(OH)2CO3 nanotubes, which are calcined to porous Al2O3 nanotubes, and the release of ammonia and carbon dioxide engineers the surface features of large surface area, numerous acidic-basic sites and suitable Zeta potentials. The synergy of these features facilitates the adsorption of the typical antibiotics ciprofloxacin and PDS activation, which is proved by experiment results and density functional theory simulation. The proposed Al2O3 nanotubes can catalyze 92–96% degradation of 10 ppm ciprofloxacin within 40 min, with chemical oxygen demand removal of 65–66% in aqueous, and 40–47% in whole including aqueous and catalysts. Ciprofloxacin at high concentration, other fluoroquinolones and tetracycline can also be effectively degraded. These data demonstrate the Al2O3 nanotubes prepared by the nature-inspired ureasolysis method has unique features and great potentials for antibiotics degradation.

Does the Environmental Air Impact the Condition of the Vomeronasal Organ? A Mouse Model for Intensive Farming.

Chemical communication in mammals is ensured by exchanging chemical signals through the vomeronasal organ (VNO) and its ability to detect pheromones. The alteration of this organ has been proven to impact animal life, participating in the onset of aggressive behaviors in social groups. To date, few studies have highlighted the possible causes leading to these alterations, and the farming environment has not been investigated, even though irritant substances such as ammonia are known to induce serious damage in the respiratory tract. The goal of this study was to investigate the environmental impact on the VNO structure. Thirty mice were split into three groups, one housed in normal laboratory conditions and the other two in confined environments, with or without the release of litter ammonia. VNOs were analyzed using histology and immunohistochemistry to evaluate the effect of different environments on their condition. Both restricted conditions induced VNO alterations (p = 0.0311), soft-tissue alteration (p = 0.0480), and nonsensory epithelium inflammation (p = 0.0024). There was glycogen accumulation (p < 0.0001), the olfactory marker protein was underexpressed (p < 0.0001), and Gαi2 positivity remained unchanged while Gαo expression was upregulated in confined conditions. VNO conditions seemed to worsen with ammonia, even if not always significantly. These murine model results suggest that the housing environment can strongly impact VNO conditions, providing novel insights for improving indoor farming systems.

Quantification of Nutrient Fluxes from Sediments of Lake Hulun, China: Implications for Plateau Lake Management

Dramatic changes in lake water conditions may induce sensitive feedback in sediments, such as the release of phosphate and ammonia. There is a lack of the understanding of sediment nutrient release fluxes and their responses to various environmental factors in plateau lakes. In this study, nutrient contents in the water and sediment, sediment nutrient release rates, and their responses to various influential factors in a steppe shallow plateau lake, Lake Hulun, China, were investigated. Sediment is a large store of nutrients, with bioavailable phosphorus of 555 mg kg−1 in surface lake sediments. If water became anoxic, sediments will release appreciable amounts of phosphate (2.1 mg P m−2 d−1) and ammonia (40.0 mg N m−2 d−1), three times greater than those in oxic conditions. Raised temperatures greatly increased sediment phosphate release rates, while the highest temperature (23 °C) slightly decreased ammonia release rates due to strong nitrification. Assuming a whole year of internal loading, ammonia and dissolved reactive phosphorus concentrations in water column would increase by an estimated 0.22 mg N L−1 and 0.01 mg P L−1, respectively, which is equal to 2 and 0.5 times of their ambient concentrations. A pH increase from 8.4 to 9.4 significantly decreased internal nitrogen and phosphorus release rates by around three times. Overall, dissolved oxygen (DO) and pH had a greater impact on sediment nutrient release from lake sediments compared to temperature, and further increased the eutrophic status in the overlying water when these three parameters varied within the normal seasonal range (temperature: 1.9–23.1 °C, DO: oxic and anoxic; pH: 7.5–9.4). With the periodic low DO and frequency of drought in the future, lake management strategies that mitigate water eutrophication, such as water diversion, may need to be considered.

Sf9 Cell Metabolism Throughout the Recombinant Baculovirus and Rabies Virus-Like Particles Production in Two Culture Systems.

This work aimed to assess the Sf9 cell metabolism during growth, and infection steps with recombinant baculovirus bearing rabies virus proteins, to finally obtain rabies VLP in two culture systems: Schott flask (SF) and stirred tank reactor (STR). Eight assays were performed in SF and STR (four assays in each system) using serum-free SF900 III culture medium. Two non-infection growth kinetics assays and six recombinant baculovirus infection assays. The infection runs were carried out at 0.1 pfu/cell multiplicity of infection (MOI) for single baculovirus bearing rabies glycoprotein (BVG) and matrix protein (BVM) and a coinfection with both baculoviruses at MOI of 3 and 2 pfu/cell for BVG and BVM, respectively. The SF assays were done in triplicate. The glucose, glutamine, glutamate, lactate, and ammonium uptake or release specific rates were quantified over the exponential growth phase and infection stage. The highest uptake specific rate was observed for glucose (42.5 × 10-12mmol cell/h) in SF and for glutamine (30.8 × 10-12mmol/cell/h) in STR, in the exponential growth phases. A wave pattern was observed for assessed analytes throughout the infection phase and the glucose had the highest wave amplitude within the 10-10mmol cell/h order. This alternative uptake and release behavior is in harmony with the lytic cycle of baculovirus in insect cells. The virus propagation and VLP generation were not limited by glucose, glutamine, and glutamate, neither by the toxicity of lactate nor ammonium under the conditions appraised in this work. The findings from this work can be useful to set baculovirus infection processes at high cell density to improve rabies VLP yield, purity, and productivity.

Zinc oxide nanoparticles coated urea enhances nitrogen efficiency and zinc bioavailability in wheat in alkaline calcareous soils.

Nitrogenous fertilizers have low efficiency in alkaline calcareous soils due to volatilization and denitrification. These losses cause economic environmental constraints. Coating of urea with nanoparticles (NPs) is an innovative strategy to improve crop yields by sustaining N availability. In the current study, zinc oxide nanoparticles (ZnO NPs) were synthesized by precipitation method and characterized for morphology and configuration, bond formation, and crystal assemblage using the X-ray diffraction and scanning electron microscope (SEM). The SEM results confirmed the size of ZnO NPs in the size range of 25 nm with cuboid shape. Urea fertilizer, coated with ZnO NPs, was applied to wheat crop in a pot trial. Two rates of ZnO NPs at 2.8 and 5.7 mg kg-1 were selected to coat the commercial urea. A batch experiment was conducted to ensure the ammonium (NH4+) and nitrate (NO3-) ions release by amending the soil with ZnO NPs coated urea and comparing with non-amended soil. The gradual release of NH4+ was observed for 21 days from the ZnO NP-coated urea. In the second part of trial, seven different treatments of coated and uncoated urea were tested on wheat crop. Urea coated with ZnO nanoparticles at 5.7 mg kg-1 improved all growth attributes and yields. The ZnO NP coated urea increased the N content shoot (1.90 g 100g-1 DW) and potentially biofortified Zn content (47.86 mg kg-1) in wheat grain. The results are indicative of viability of a novel coating for commercial urea that will not only reduce N losses but also supplement Zn without additional cost of labor.

Direct Synthesis of Ammonia from Nitrate on Amorphous Graphene with Near 100% Efficiency.

Ammonia is an indispensable commodity in the agricultural and pharmaceutical industries. Direct nitrate-to-ammonia electroreduction is a decentralized route yet challenged by competing side reactions. Most catalysts are metal-based, and metal-free catalysts with high nitrate-to-ammonia conversion activity are rarely reported. Herein, it is shown that amorphous graphene synthesized by laser induction and comprising strained and disordered pentagons, hexagons, and heptagons can electrocatalyze the eight-electron reduction of NO3 - to NH3 with a Faradaic efficiency of ≈100% and an ammonia production rate of 2859 µg cm-2 h-1 at -0.93 V versus reversible hydrogen electrode. X-ray pair-distribution function analysis and electron microscopy reveal the unique molecular features of amorphous graphene that facilitate NO3 - reduction. In situ Fouriertransform infrared spectroscopy and theoretical calculations establish the critical role of these features in stabilizing thereaction intermediates via structural relaxation. The enhanced catalytic activity enables the implementation of flow electrolysis for the on-demand synthesis and release of ammonia with >70% selectivity, resulting in significantly increased yields and survival rates when applied to plant cultivation. The results of this study show significant promise for remediating nitrate-polluted water and completing the NOx cycle.