Amino Acid Profile Research Articles

Protein complex structure modeling by cross-modal alignment between cryo-EM maps and protein sequences

Cryo-electron microscopy (cryo-EM) technique is widely used for protein structure determination. Current automatic cryo-EM protein complex modeling methods mostly rely on prior chain separation. However, chain separation without sequence guidance often suffers from errors caused by cross-chain interaction or noise densities, which would accumulate and mislead the subsequent steps. Here, we present EModelX, a fully automated cryo-EM protein complex structure modeling method, which achieves sequence-guiding modeling through cross-modal alignments between cryo-EM maps and protein sequences. EModelX first employs multi-task deep learning to predict Cα atoms, backbone atoms, and amino acid types from cryo-EM maps, which is subsequently used to sample Cα traces with amino acid profiles. The profiles are then aligned with protein sequences to obtain initial structural models, which yielded an average RMSD of 1.17 Å in our test set, approaching atomic-level precision in recovering PDB-deposited structures. After filling unmodeled gaps through sequence-guiding Cα threading, the final models achieved an average TM-score of 0.808, outperforming the state-of-the-art method. The further combination with AlphaFold can improve the average TM-score to 0.911. Analyzes conducted by comparing some EModelX-built models and PDB structures highlight its potential to improve PDB structures. EModelX is accessible at https://bio-web1.nscc-gz.cn/app/EModelX.

Extraction and Nutritional Value of Soybean Meal Protein Isolate

The increasing protein demand driven by global population growth necessitates the search for an alternative protein source. Soybean meal (SM), with approximately 47–49% proteins, is a viable option. Soymeal protein isolate (SMPI) is a cost-efficient protein source with a well-balanced amino acid profile, making it suitable for addressing this demand. This study attempts to address the lack of information regarding the extractability and nutritional potential of SMPI obtained utilizing a weak base and recoverable solvent. In this work, the structural and compositional studies of SMPI, as well as the aqueous extractability of ammonium hydroxide (NH4OH), are investigated. Furthermore, we examined the effects of heat treatment during oil extraction, where a comparison between protein isolates from soymeal and whole soybeans was carried out. The maximum extraction yield of 24.1% was achieved by increasing the concentration of NH4OH from 0.25–1%. In comparison to the soy protein isolate from whole soybeans (SPI), the compositional analysis report revealed that SMPI had higher levels of crude protein, moisture, and ash content, but lower levels of fat and carbohydrates. Furthermore, the examination of the amino acid composition confirms the existence of vital amino acids in SMPI. The amino acid score indicates that methionine, lysine, and threonine are the limiting amino acids. SMPI and SPI share structural and functional group similarities, as demonstrated by Fourier-transform infrared spectroscopy. Gel electrophoresis using sodium dodecyl sulfate–polyacrylamide shows that the protein molecular weight distributions of SPI and SMPI are similar. This in-depth evaluation emphasizes the advantages of SM by advocating its application in other sectors beyond conventional animal feed, such as nutritional supplements and bio-based products, and by improving the environmental sustainability and global food chains.

Protein Fractions of Jackfruit Leaf Flour and Protein Concentrate: Amino Acid Profile, Functional Properties and Thermal Analysis

Protein Fractions of Jackfruit Leaf Flour and Protein Concentrate: Amino Acid Profile, Functional Properties and Thermal Analysis

Can endogenous ethylene glycol production occur in humans? A detailed investigation of adult monozygotic twin sisters

Introduction To the best of our knowledge, clinically significant endogenous ethylene glycol production has never been reported in humans, very seldom reported in other animals or microorganisms, and then only under rare and specific conditions. We describe the detailed investigations we undertook in two adult monozygotic twin sisters to ascertain whether they were producing endogenous ethylene glycol. Methods Two previously healthy monozygotic adult twin sisters presented with recurrent episodes of apparent ethylene glycol poisoning beginning at age 35, requiring chronic hemodialysis to remove ethylene glycol and its metabolites as well as to restore metabolic homeostasis. The sisters denied ingestion or exposure to ethylene glycol. At their request, they were admitted to hospital under strict supervision to exclude surreptitious ingestion of ethylene glycol and to evaluate the need for treatment. Hemodialysis was withheld during this prospective study. Twin A was admitted for 14 days and twin B for 11 days. Serial biochemical analyses were performed in blood and urine. Clinical exome sequencing and mitochondrial deoxyribonucleic acid sequencing were also completed. Results In both twins, ethylene glycol was detected in urine, along with intermittent increases in concentrations of lactate, glycolate, and glycine in blood and/or urine. Blood ethylene glycol concentrations, however, remained <62 mg/L (<1 mmol/L) but became positive soon after discharge. The oxalate concentration remained normal in blood and urine. Plasma and urine amino acid profiles showed intermittent small increases in glycine, serine, taurine, proline, and/or alanine concentrations. Exome sequencing and mitochondrial deoxyribonucleic acid sequencing were non-diagnostic. Neither twin has been admitted with metabolic acidosis nor ethylene glycol poisoning since chronic hemodialysis was started. Twin A developed a calcium oxalate dihydrate lithiasis. Discussion Mitochondrial disease, methylmalonic/propionic/isovaleric aciduria, primary hyperoxaluria, and analyte error were all excluded in these twins, as were obvious common environmental exposures. Conclusion Detailed investigations were performed in adult monozygotic twin sisters to ascertain whether they were producing endogenous ethylene glycol. Alternative explanations were excluded to the very best of our efforts and knowledge. Global metabolomics, gut microbiome analyses, and whole genome sequencing are pending.

Citric Acid by-Product Fermentation by Bacillus subtilis I9: A Promising Path to Sustainable Animal Feed

Citric acid by-products in animal feed pose a sustainability challenge. Bacillus species are commonly used for fermenting and improving the nutritional quality of feedstuffs or by-products. An experiment was conducted to enhance the nutritional value of citric acid by-products through fermentation with Bacillus subtilis I9 for animal feed. The experiment was carried out in 500 mL Erlenmeyer flasks with 50 g of substrate and 200 mL of sterile water. Groups were either uninoculated or inoculated with B. subtilis I9 at 107 CFU/mL. Incubation occurred at 37 °C with automatic shaking at 150 rpm under aerobic conditions for 0, 24, 48, 72, and 96 h. Inoculation with B. subtilis I9 significantly increased Bacillus density to 9.3 log CFU/mL at 24 h (p &lt; 0.05). CMCase activity gradually increased, reaching a maximum of 9.77 U/mL at 72 h. After 96 h of fermentation with inoculated B. subtilis I9, the citric acid by-product exhibited a significant decrease (p &lt; 0.05) in crude fiber by 10.86%, hemicellulose by 20.23%, and cellulose by 5.98%, but an increase in crude protein by 21.89%. Gross energy decreased by 4% after inoculation with B. subtilis in comparison to the uninoculated control (p &lt; 0.05). Additionally, the non-starch polysaccharide (NSP) degradation due to inoculation with B. subtilis I9 significantly reduced (p &lt; 0.05) NSP by 24.37%, while galactose, glucose, and uronic acid decreased by 22.53%, 32.21%, and 18.11%, respectively. Amino acid profile content increased significantly by more than 12% (p &lt; 0.05), including indispensable amino acids such as histidine, isoleucine, lysine, methionine, phenylalanine, tryptophan, and valine and dispensable amino acids like alanine, aspartic acid, glutamic acid, glutamine, glycine, proline, and tyrosine. Furthermore, citric acid by-products inoculated with B. subtilis I9 exhibited changes in the cell wall structure under scanning electron microscopy, including fragmentation and cracking. These results suggest that fermenting citric acid by-products with B. subtilis I9 effectively reduces dietary fiber content and improves the nutritional characteristics of citric acid by-products for use in animal feed.

Chitosan Priming Ameliorates Protein and Amino Acid Profiles in Red Kidney Bean under Drought Stress

Chitosan Priming Ameliorates Protein and Amino Acid Profiles in Red Kidney Bean under Drought Stress

Evaluation of the role of EGFR exon 19 747-750 deletion mutation and plasma amino acid profile in the development of lung cancer.

Lung cancer (LC) is the most common form of cancer in the world. Of the proteins involved in cell differentiation and proliferation, the epidermal growth factor receptor (EGFR) is among the most significant. Amino acids play a crucial role in cell physiology as metabolic regulators. The benefits of liquid biopsies are their non-invasive nature, ease of collection, and ability to depict the entire tumor's status. The present study is designed to detect the relation between the EGFR exon 19 747-750 deletion mutation and lung cancer and investigate the patterns of alterations of plasma-free amino acids (PFAA) in lung cancer patients of different histopathological types and stages as biomarkers for early detection of lung cancer. The study sample comprised 60 lung cancer patients and 60 age- and sex-matched healthy individuals as the control group. Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were used to examine the EGFR exon 19 747-750 deletion mutation, and an AA analyzer was used to quantify the plasma free amino acid (PFAA) profile. Compared with controls, LC patients had significantly higher levels of three AAs and significantly lower levels of fifteen AAs. Thirteen AAs varied significantly between stages I and II. In the lung cancer group, the percentage of cases of mutant EGFR exon-19 deletion increased to 30% from 13.3% in the control group. The histological forms of lung cancer did not significantly differ in this rise. Valine and citrulline plasma levels were substantially greater in the mutant than in the wild-type. Lysine, histidine, and methionine were the independent predictors of the LC group in multivariate analysis. Lung cancer development is influenced by the EGFR exon 19 747-750 deletion mutation, and the prognosis and early prediction of lung cancer are greatly affected by the amino acid profile concentrations.

Amino Acid Profile and Proximate Analysis of Fermented African Locust Bean (Parkia biglobosa) Seeds

Amino Acid Profile and Proximate Analysis of Fermented African Locust Bean (Parkia biglobosa) Seeds

Factors Affecting Yeast Digestibility and Immunostimulation in Aquatic Animals.

The aquafeed industry increasingly relies on using sustainable and appropriate protein sources to ensure the long-term sustainability and financial viability of intensive aquaculture. Yeast has emerged as a viable substitute protein source in the aquaculture sector due to its potential as a nutritional supplement. A substantial body of evidence exists to suggest that yeast has the potential to act as an effective immune-stimulating agent for a range of aquaculture fish species. Furthermore, the incorporation of yeast supplements and feed additives has the potential to bolster disease prevention, development, and production within the aquaculture sector. Except for methionine, lysine, arginine, and phenylalanine, which are typically the limiting essential amino acids in various fish species, the various yeast species exhibit amino acid profiles that are advantageous when compared to fishmeal. The present review considers the potential nutritional suitability of several yeast species for fish, with particular attention to the various applications of yeast in aquaculture nutrition. The findings of this study indicate that the inclusion of yeast in the diet resulted in the most favorable outcomes, with improvements observed in the overall health, growth performance, and nutritional condition of the fish. Digestibility, a key factor in sustainable feed development, is discussed in special detail. Additionally, this review addresses the utilization of yeast as an immunostimulating agent for fish and its digestion in fish. Furthermore, the research emphasizes the necessity of large-scale production of yeast as a substitute for fishmeal in aquaculture.

Extended photoperiods enhance the production and water treatment performance of algal-bacterial bioflocs from aquaculture wastewater

Reducing pollution and converting wastewater nutrients into useful biomass are major challenges of intensive aquaculture. We investigated the nitrogen and phosphorus metabolism, nutrient composition, and microbial community of algal-bacterial bioflocs (ABBs) produced under glucose addition and different photoperiods. This biomass was used for aquaculture wastewater treatment for 18 days. ABBs produced under longer photoperiods were smaller and more numerous, which facilitated nitrogen recovery and reduced nitrogen loss. Additionally, nitrogen and phosphorus-metabolizing enzyme activities, as well as the activity of overall microorganisms were positively correlated with the light duration. The removal rates of COD (96.89 %), DTN (93.57 %), and DTP (93.67 %) from wastewater by ABBs were highest when the photoperiod was 16L:8D during the production period. Moreover, the crude protein content (41.77 ± 0.11 %) was highest under a photoperiod of 12L:12D, and the rich amino acid and fatty acid profile of the ABB under this photoperiod enhanced its utility as a feed additive for aquaculture species. The high-throughput sequencing analysis revealed a high abundance of Hydrogenophaga at the genus level, highlighting its denitrification capacity of ABBs under the 16L:8D photoperiod. Redundancy analysis (RDA) demonstrated that photoperiod, as a key driver in the formation of microbial communities in ABBs, exerted a direct influence on water treatment efficiency and nutrient accumulation. This study provides new insights into the effects of photoperiod on the properties of ABBs, which has implications for the treatment and subsequent application of aquaculture wastewater.

Potential and application of tandem mass spectrometry (MS/MS) in the analysis and identification of novel bacteriocins: a review

SummaryBacteriocins are antimicrobial peptides synthesised ribosomally by Gram‐positive or Gram‐negative bacteria to gain a competitive advantage. The majority of bacteriocins are derived from Gram‐positive bacteria, with lactic acid bacteria being the most common source. Because they are considered ‘natural’, there is currently significant development of bacteriocins for application as food preservative agents. As a preservative agent, bacteriocin activity is highly dependent on purity, down to the amino acid profile and sequence. Therefore, bacteriocin identification is important. Currently, MS is a cutting‐edge tool in bacteriocin identification. This method has high selectivity, sensitivity and resolution. To the best of our knowledge, systematic reviews focusing on the application of MS for bacteriocin identification are currently limited. In light of this, the objective of this study is to provide a comprehensive review and summary of MS technologies in bacteriocin research, with a particular focus on the discovery and characterisation of novel sources of bacteriocin. Additionally, studies related to the discovery of bacteriocins from various sources, their role as antimicrobial agents, and their synthesis are emphasised. Thus, this study presents a comprehensive analysis of the advantages, limitations, and future perspectives of the methods employed.

Expression of concern: "Okra seeds proteins: Amino acid profile, free radical scavenging activities and inhibition of diabetes and hypertensive converting enzymes indices" [Measurement: Food, Volume 11, September 2023, 100101]

Expression of concern: "Okra seeds proteins: Amino acid profile, free radical scavenging activities and inhibition of diabetes and hypertensive converting enzymes indices" [Measurement: Food, Volume 11, September 2023, 100101]

Influence of Various Methods of Processing Soybeans on Protein Digestibility and Reduction of Nitrogen Deposits in the Natural Environment – A Review

Abstract Soybean meal (SBM) is a significant source of protein for poultry due to its high concentration of protein and amino acids profile. The primary objective of SBM processing is the reduction and deactivation of anti-nutritional factors (ANFs) to enhance nutrient digestibility. However, eliminating ANFs does not necessarily correlate with increased protein and amino acid content. Several processing methods, such as soaking, cooking, and commercial techniques, improve SBM protein digestibility. To sustain feeding qualities, soybeans must undergo heating to remove inherent chemicals that interfere with poultry digestion. However, both under and over-processing can impair meal digestibility. Extrusion heating significantly contributes to protein and starch digestibility while reducing certain ANFs. Thermomechanical and enzyme-assisted procedures reduce heat-resistant proteins more efficiently than extraction methods, producing soy protein concentrate (SPC) and fermentation. These techniques reduce ANFs in SBM, activating digestive enzymes and alleviating digestive tract pressure, leading to decreased endogenous protein deficits. Exogenous enzyme supplementation is a biotechnological approach for enhancing the nutritional content of SBM and certain other protein-rich products. However, different processing methods not only affect protein digestibility but also impact poultry production, gastrointestinal health, and the environment due to higher nitrogen excrement. Ammonia (NH3) emissions are a significant environmental concern in chicken farming, resulting from uric acid production during protein consumption. It has a negative impact on the environment and the health of birds/humans. This review investigates the impact of several processing techniques on the digestibility of SBM protein and the reduction of N2 regarding one health concept. Future research should focus on identifying optimal processing methods maximizing protein digestibility while minimizing environmental nitrogen impacts.

Meals Containing Equivalent Total Protein from Foods Providing Complete, Complementary, or Incomplete Essential Amino Acid Profiles do not Differentially Affect 24-h Skeletal Muscle Protein Synthesis in Healthy, Middle-Aged Women

BackgroundDietary protein quality can be assessed by skeletal muscle protein synthesis (MPS) stimulation. Limited knowledge exists on how consuming isonitrogenous meals with varied protein qualities affects postprandial and 24-h MPS. ObjectivesWe assessed the effects of protein quality and complementary proteins on MPS. We hypothesized that meals containing a moderate amount of high-quality, complete protein would stimulate postprandial and 24-h MPS. Meals containing 2 complementary, plant-based incomplete proteins would stimulate MPS less, and meals containing plant-based incomplete proteins at each meal, but complementary over 24 h would not stimulate MPS. Methods: This quasi-experimental study included a randomized, crossover design to assess protein quality and a nonrandomized low-protein control. We measured postprandial and 24-h MPS responses of healthy middle-aged women (n = 9, age 56 ± 4 y), to 3 dietary conditions: isonitrogenous meals containing 23 g protein/meal from 1) complete protein (lean beef), 2) 2 incomplete, but complementary protein sources (navy/black beans and whole wheat bread), and 3) single incomplete protein sources (black beans or whole wheat bread at 1 meal), but providing a complete amino acid profile over 24 h. In the low-protein group women (n = 8, 54 y) consumed a single breakfast meal containing 5 g of protein. Venous blood and vastus lateralis samples were obtained during primed, constant infusions of L-[ring-13C6]phenylalanine to measure mixed muscle fractional synthetic rates (FSR). ResultsMeals containing complete, complementary, or incomplete proteins did not differentially influence FSR responses after breakfast (P = 0.90) or 24 h (P = 0.38). At breakfast, the complete (P = 0.030) and complementary (P = 0.031) protein meals, but not the incomplete protein meal (P = 0.38), had greater FSR responses compared with the low-protein control meal. ConclusionsIsonitrogenous meals containing a moderate serving of total protein from foods providing complete, complementary, or incomplete essential amino acid profiles do not differentially stimulate muscle protein synthesis after a meal and daily. Trial registration numberThis clinical trial was registered at clinicaltrials.gov as NCT03816579. URLhttps://www.clinicaltrials.gov/ct2/show/NCT03816579?term=NCT03816579&draw=2&rank=1.

Investigation of amino acid and fatty acid profiles of Japanese diets using the food exchange lists for diabetes diet

Investigation of amino acid and fatty acid profiles of Japanese diets using the food exchange lists for diabetes diet

Volvariella volvacea (paddy straw mushroom): A mushroom with exceptional medicinal and nutritional properties

Volvariella volvacea, commonly referred to as paddy straw mushroom, is renowned for its remarkable medicinal and nutritional properties. This mushroom, part of the family Pluteaceae, thrives in tropical and subtropical regions and is highly esteemed for its distinctive flavor and substantial health benefits. The fruiting body of V. volvacea is a rich source of bioactive compounds, including antioxidant enzymes, terpenes, polypeptides, sugars, phenolics, and flavonoids. These compounds exhibit an extensive range of therapeutic activities such as anti-tumor, anti-microbial, antioxidant, anti-malarial, anti-cancer, anti-inflammatory, and anti-allergic effects. Nutritionally, V. volvacea is an excellent source of carbohydrates, proteins, fibers, ascorbic acid, and essential minerals. It also boasts a comprehensive profile of amino acids, including valine, arginine, glutamine, serine, aspartic acid, leucine, isoleucine, tyrosine, asparagine, lysine, cystine, proline, glycine, tryptophan, methionine, phenylalanine, threonine, and histidine. This review emphasizes the significant medicinal and nutritional potential of V. volvacea, advocating its inclusion as a functional food to enhance human health and well-being. By highlighting its diverse bioactive compounds and therapeutic benefits, this review aims to foster greater recognition and utilization of paddy straw mushroom in both dietary and medicinal applications.

Biosynthetic capabilities of Antarctic yeast Sporobolomyces roseus AL103: Temperature influence on intracellular metabolites and characterization of the exopolysaccharide

The purpose of the study was to investigate the biosynthetic properties of the Antarctic yeast strain Sporobolomyces roseus AL103 in response to temperature changes, to perform intracellular metabolic profiling, and to reveal the chemical and functional characteristics of the synthesized exopolysaccharide (EPS). The results show that the yeast strain needed a shorter time to reach a stationary phase at 22 °C contrary to that of 5 °C. An NMR analysis revealed differences in metabolic profiles of amino acids, glucose, trehalose, glycerol, uridine, etc. EPS (2.9 g/L) was characterized by high–molecular-weight with total carbohydrate, uronic acids, and protein content of 66 %, 10.5 %, and 2.5 %, respectively. Mannose (74 mol%) and galactose (19 mol%) were the major constituents. The FT-IR data suggested the presence of β-(1–4)-mannan. DSC thermogram, WVTR, mechanical properties, and moisture sorption of the EPS film showed thermal stability up to 220 °C and hydrophilic behavior. The newly obtained polymer film was studied for the first time and the data showed possibilities for its successful application as a film-forming material in the preparation of packaging materials. In conclusion, the temperature influenced the metabolic profile of the Antarctic yeast producer. The biotechnological process could be directed to obtain the target intracellular or extracellular metabolites.

Nutritional properties of commercially important cephalopods from the south-west coast of India

Cephalopods are widely regarded as healthy and nutritionally rich seafood resources. This study focuses on analysing the nutritional composition of six commercially important edible cephalopod species viz., Cistopus indicus, Amphioctopus marginatus, Amphioctopus aegina, Sepia pharaonis, Sepia elliptica and Sepia aculeata, from the Malabar coast of the Indian peninsula. The selected cephalopods were determined to be a rich source of protein ranging from 12.41 to 19.02 g 100 g-1. The amino acid profile demonstrated a balanced ratio of essential to non-essential amino acids (0.93-1.63) with a higher concentration of essential amino acids, attributing enhanced biological value of proteins in these cephalopods. S. pharaonis recorded the highest arginine-to-lysine ratio (~1.77), indicating excellent cholesterolemic index. Notably, octopus species had the lowest total cholesterol content (~100 mg 100 g-1) compared to cuttlefish species which recorded higher cholesterol levels (~160 mg 100 g-1). A. marginatus recorded more significant quantities of polyunsaturated fatty acids (PUFA) (36.85% total fatty acids) among all the species studied. Octopus species exhibited significantly greater Σn-3/Σn-6 ratio than cuttlefish species. The ideal atherogenicity/thrombogenicity indices (&lt;1.0) in the edible tissues of the studied cephalopods qualify these species as potentially healthy food. A. aegina was found to exhibit greater vitamin E (α-tocopherol) contents (17.84 IU), whereas ascorbic acid was pronounced more in A. marginatus (47.06 μg 100 g-1). The higher vitamin D3 content in A. aegina (96.23 μg 100 g-1) among the species studied, highlights its potential role in preventing osteoporosis in adults. Among microelements such as iron and zinc were found in significant quantities in the cephalopod species studied. The findings from the current study provide valuable insights into the nutritional benefits of these species, positioning them as promising candidates for the seafood industry as possible healthy dietary options. Keywords: Amino acid composition, Atherogenicity index, Cephalopods, Fatty acid composition, Thrombogenicity index

Fractionation, characterization, and assessment of nutritional and immunostimulatory protein-rich polysaccharide-protein complexes isolated from Lentinula edodes mushroom

This study aimed to fractionate and characterize the protein-rich polysaccharide-protein (PSP) complexes from a well-known edible mushroom, Lentinula edodes, and assess their nutritional and immunostimulatory properties. Crude PSP isolated from the mushroom water extract was purified by anion exchange chromatography, yielding fractions PSP-F1 and PSP-F2 containing 66.1 % and 74.0 % protein, respectively. Both fractions exhibited primarily β-sheet and random-coil protein structures, though the crude PSP fraction exhibited an additional α-helix structure. On SDS-PAGE, PSP-F1 showed two molecular weight bands, one below 10 kDa and another at 34 kDa, and PSP-F2 showed several bands, one below 10 kDa and others between 34 and 95 kDa. The nutritional value of essential and non-essential amino acid profiles was in the order of PSP-F2 > PSP-F1 > crude PSP; the amino acid ratio coefficient values of the crude PSP, PSP-F1, and PSP-F2 were 63 %, 67 %, and 72 %, respectively. The combination of PS and PSP fractions exhibited stronger immunoactivity than PSP-F1 or PSP-F2 alone. PSP-F2 showed a higher immunostimulatory activity than PSP-F1 in RAW264.7 cell culture. PSP-F2 was also more abundant of easily absorbed high-quality proteins. The results provide useful references for dietary and medicinal uses of PSP fractions in L. edodes and other edible mushrooms.

Study of the jellyfish Rhizostoma pulmo hydrolysis and characterization of the obtained product

The purpose of this work is to develop technological parameters for the hydrolysis of barrel jellyfish Rhizostoma pulmo involving the use of enzymatic agents «Enzy-mix» and alcalase, available through the domestic market, as well as to investigate the amino acid and mineral composition of the resultant hydrolysate.Methods used: the process of hydrolysis has been assessed based on the changes in peptide concentrations in the hydrolysate, the content of amino nitrogen, and the degree of hydrolysis. To investigate its chemical, amino acid, and mineral composition, the standard methods were used. Novelty: the use of jellyfish for hydrolysis after its thawing and the hydrolysis procedure not involving the addition of water into the raw material subjected to hydrolysis present a novel approach.Result: the optimal regime for hydrolysis of barrel jellyfish Rh. pulmo after its thawing has been identified, which was characterized by the use of 1% alcalase at the temperature 55±2 °C and duration of 1 h. It has been shown that alcalase is more effective in terms of amino nitrogen accumulation than enzymatic agent «Enzy-mix». The values of amino nitrogen after 1 h of hydrolysis involving the use of 1% alcalase were higher than the values of the same parameter obtained during the 3-hour hydrolysis involving 1.5% «Enzy-mix» at the temperature 40±2 °C. The hydrolysate, after its concentration to the dry matter exceeding 30%, contains no less than 15.1±0.59% of protein represented by 48% of essential amino acids, out of which leucine and isoleucine prevail (15 %). Among non-essential amino acids, the most prominent was glycine (17 %). For all essential amino acids, except for valine, the amino acid score exceeds 100%. In humans, consumption of around 20 g of the hydrolysate covers the daily physiological requirement of magnesium.Practical significance: the obtained data on the amino acid profile and mineral composition of the barrel jellyfish Rh. pulmo hydrolysate provide the basis for its consideration as a promising ingredient for the functional nutrition products.