Amberlite XAD-2 Research Articles

Comparative solid phase extraction study on the U(VI) preconcentration by using immobilized thermotolerant Bacillus vallismortis and Bacillus mojavensis

Bacillus vallismortis and Bacillus mojavensis were loaded onto Amberlite XAD-4 resin and used for solid phase extraction (SPE) of uranium(VI). A quick and simple UV–Vis spectrophotometric method was used to determine U(VI) ion. The best experimental conditions were determined as being a pH of 5.0; a sample flow rate of 2.0 mL min−1; 200.0 mg of biosorbent; 800 mg of Amberlite XAD-4, and 5.0 mL of 1 mol L−1 HCl as desorption solution for both immobilized bacteria. The preconcentration factors were achieved as 80 for both solid phase extractor. The developed methods were validated by applying to reference water and tea samples.

On the effect of the operating parameters for two-phase olive-oil washing wastewater combined phenolic compounds recovery and reclamation by novel ion exchange resins

Abstract Olive oil is produced by a technological procedure based on physical operations, without use of chemicals. This industry is concerned to make the whole process environmentally friendly, which includes the treatment of the wastewater produced in the mills. In the present work, the isolation, purification and recovery of high-added value phenolic compounds from two-phase olive-oil washing wastewater (OOWW) and the simultaneous effluent treatment by a ‘green process’ based on resins adsorption/ion exchange (strong-base anionic Amberlyst A26®, weak-base anionic Dowex 66® and non-ionic macroreticular Amberlite XAD4®) was studied. Results showed the resins performances were optimal at the raw effluent pH and ambient temperature conditions, thus no acidification or basification, nor cooling or heating, would be necessary: the effluent could be directly driven from the exit of the vertical centrifuges at the outlet temperature and raw pH to the proposed resins process, boosting the economic feasibility. The strong anionic resin ensured optimum results: upon 150 min maximum contact time and 60–80 g resin/L, a final treated effluent containing only 8.7 mg/L total phenolic compounds was obtained. The effluent could be partially discharged on suitable terrains or disposed to biological treatments, avoiding phytotoxicity or inhibition due to the phenolic content. The obtention of this concentrated pool of added-value antioxidant compounds for food, cosmetics, pharmaceutical and biotechnological industrial sectors could help counter-balance the economic feasibility of the reclamation process.

VALIDATION OF HEPATOPROTECTIVE AND ANTIOXIDANT ACTIVITY OF FOLK LORE KNOWLEDGE OF PURIFIED ANTHOCYANIN FROM CELL SUSPENSION CULTURE OF CLERODENDRON INFORTUNATUM L.: A SEARCH

ABSTRACT Objective: To validate the hepatoprotective and antioxidant activity of purified anthocyanin extracted from the cell suspension culture of Clerodendron infortunatum Linn. Methods: A protocol has been developed for the induction of callus proliferation from leaf and nodal explants of C. infortunatum. The explants were inoculated on MS medium supplemented with diverse combinations of 2,4-D and BAP for triggering callus formation. Subsequently, the green compact callus has been sub-cultured in the medium fortified with 2,4-D and Kinetin for anthocyanin synthesis. Cell suspension culture was also established and the elicitor, salicylic acid was used for triggering anthocyanin synthesis. Three different chromatographic columns (solid phase extraction by Sepharose C18 column, Oasis-MCX and Amberlite XAD 7 + Sephadex LH 120 sorbents) were employed to purify the in vitro synthesized anthocyanin from cell suspension cultures. For purity evaluation HPLC and molar absorptivity assay were used. Further, hepatoprotective and antioxidant activity was evaluated comparing with silymarine, as standard in rats. In vitro antioxidant scavenging activity was analysed by DPPH, FRAP and ORAC assay.Results: After 1 month, the leaf explants yielded remarkable green compact callus on MS medium containing 2.0 mg/l BAP and 0.5 mg/l 2,4-D. Salicylic acid enhanced anthocyanin synthesis. The mean purity values obtained by HPLC were 90.9% ± 1.9 and 80.60% ±2.3 for Oasis MCX, Amberlite XAD-7 + Sephadex LH-20 column respectively. However, the purity calculated by molar absorptivity was found to be less. The highest purity achieved using molar absorptivity analysis was with MCX cartidges i.e., 85.9 ± 3.8%. HPLC yielded 12 anthocyanin fractions. Remarkable antioxidant scavenging activity was noticed as revealed by DPPH, FRAP and ORAC assay. The hepatoprotection activity (25, 50, 100 mg/100g b.w) was compared with silymarine (25 mg/kg b.w) against CCl4 induced toxicity. Anthocyanin extract improved the AST, ALT and recovered the activity of kidney function by decreasing the urea and creatinine content. In addition, the administration of anthocyanin significantly inhibited the oxidative stress via its scavenging of the reactive oxygen species formed by CCl4 stress. Further, a decrease in the MDA, H2O2, NO accumulation and increase of GSH content were noticed. Similarly, improved lipid profiles, LDL and HDL levels were also observed suggesting that anthocyanin significantly suppress the toxicity via its activation of antioxidant enzymes (GST, CAT and SOD).Conclusion: The overall results showed that the purified anthocyanin of C. infortunatum function as an antioxidant and there by hepatoprotective protection against CCl4 inducedtoxicity in animal models.

The adsorption-desorption behavior of strontium ions with an impregnated resin containing di (2-ethylhexyl) phosphoric acid in aqueous solutions

An Amberlite XAD-4 resin impregnated with di(2-ethylhexyl)phosphoric acid was prepared and its adsorption-desorption behaviors with Sr(II) ions under various conditions was examined. The resin was characterized by fourier transform infrared and thermal analysis techniques. The effects contact time, temperature, pH, interfering ions and eluants were studied. Results showed that adsorption of Sr (II) well fitted with pseudo-second-order kinetic model. The equilibrium adsorption data of Sr (II) on the impregnated resin were analyzed by Jossens, Weber-van Vliet, Redlich-Peterson and Fritz-Schlunder models to find out desirable equilibrium condition. Among them, the Fritz-Schlunder model best fitted to the experimental data. The maximum sorption capacity of impregnated resin amounted to 0.45 mg/ g at pH 8.0 and 20oC.

Adsorption of short chain carboxylic acids from aqueous solution by swellable organically modified silica materials

The adsorption of C2–C6 short chain carboxylic acids was measured for swellable organically modified silicas (SOMS), hydrophobic polysilsesquioxane based materials prepared via the sol–gel process. Adsorption was measured for SOMS materials that were either hydrophobic or incorporated variable amounts of quaternary amine groups to serve as ion exchange sites. Batch equilibrium experiments were performed for carboxylic acids and alcohols to standard hydrophobic SOMS. Results show that SOMS has partition coefficients for short chain alcohols that are approximately 3× greater than liquid–liquid extraction into octanol, and 10× greater for the extraction of short chain carboxylic acids. Affinity for valeric acid was similar to commercially available Amberlite XAD-4 resins, however the organosilica derived sorbent had a 3× higher total adsorption capacity which was attributed to the ability of the pores to expand. Column experiments using SOMS were used to measure breakthrough curves for valeric acid across a wide range of concentrations. The isotherm for dynamic adsorption was linear indicating that capacity to adsorb valeric acid was controlled by equilibrium and not limited by set pore volume capacity. Addition of quaternary amine sites to SOMS improved adsorption affinity of valeric acid at low concentrations, but those modifications reduced the swelling of the matrix and lowered capacity at higher concentrations. Overall, SOMS was found to be poorly suited to extract organic acids at concentrations < 10 mM, but has high capacity for adsorption at higher concentrations (up to 700 mg/g). Adsorbed valeric acid could be recovered by solvent rinse.

Optimisation of XAD extraction methodology for the assessment of biodegradation potential of 14C-phenanthrene in soil

Abstract This study investigated the use of a hydrophobic resin, amberlite XAD, as a tool for assessing the biodegradation potential of 14C-phenanthrene in soil. The method was optimised in terms of soil/XAD ratio, shaking, extraction time and eluting solvent. The most effective method was then tested on selected XADs, and the performance compared with cyclodextrin (HP- β -CD) and dichloromethane (DCM) extractions suitability to predict phenanthrene biodegradation in soil over 100 d. Results showed that the optimum conditions for the XAD extraction technique are a 2:1 soil/XAD ratio, 100 rpm mixing for 22 h and elution using a DCM:methanol solution (1:1). Mineralisation of 14C-phenanthrene was accurately predicted by HP- β -CD ( r 2 = 0 . 990 , slope = 0.953, intercept = 1.374) and XAD-4 extractions ( r 2 = 0 . 989 , slope = 0.820, intercept = 6.567), while DCM overestimated the bioaccessibility of 14C-phenanthrene ( r 2 = 0 . 999 , slope = 1.328, intercept = − 49 . 507 ). This investigation showed that XAD extraction can be considered a suitable non-exhaustive technique for estimating biodegradability of phenanthrene in soil.

The Removal of Cd and Cu from Aqueous Solution Using Sorbents Siirt Peanut Shells Immobilized on Amberlite XAD-4

The Removal of Cd and Cu from Aqueous Solution Using Sorbents Siirt Peanut Shells Immobilized on Amberlite XAD-4

Ultrasound-assisted extraction and adsorption of polyphenols from Ginger Rhizome (Zingiber officinale)

ABSTRACTUltrasound-assisted extraction followed by adsorption and desorption was performed successively for efficient recovery of polyphenols from ginger rhizome (Zingiber officinale). The maximum degree of extraction was observed in case of methanol (0.55 mg/ml) followed by acetone (0.52 mg/ml) and ethanol (0.37 mg/ml). At optimized conditions, the polyphenol recovery was found to be 19.2 mg. Amberlite XAD4 had shown the highest adsorption capacity (0.89 mg/ml). Kinetic studies indicated the adsorption equilibrium to be about 20 min. Adsorption capacity was achieved at 25 ± 2°C, and adsorption process exhibited exothermic nature. Freundlich isotherm fitted the data well. Ethanol had shown best desorption efficiency (93.44%).

Polyphenolic content, invitro antioxidant activity and chemical composition of extract from Nephelium lappaceum L. (Mexican rambutan) husk.

To determinate the recovery of total polyphenolic compounds content, invitro antioxidant activity and HPLC/ESI/MS characterization of extract from Nephelium lappaceum L. (Mexican rambutan). The rambutan husk extract was obtained by aqueous extraction and a polyphenolic fraction was recovered using Amberlite XAD-16. The total polyphenolic compounds content was determined by the Folin Ciocalteu and butanol-HCI methods. Invitro antioxidant activity was performed using ABTS and ferric reducing antioxidant power methods. Mexican rambutan husk showed a total polyphenolic content of 582mg/g and an evident antioxidant activity by ABTS and ferric reducing antioxidant power analysis. The HPLC/ESI/MS assay allowed the identification of 13 compounds, most of which belong to ellagitannins. Geraniin, corilagin and ellagic acid were present in the sample; the mineral composition was also evaluated. Rambutan husk cultivated in Mexico is a promising source for the recovery of added value bioactive compounds with antioxidant activity, which have potential applications as bioactive antioxidant agents for the treatment of diseases.

Synthesis, characterization and physicochemical studies of new chelating resin 1, 8-(3, 6-dithiaoctyl)-4-polyvinylbenzenesulphonate (dpvbs) and its metallopolymer Cu(II), Ni(II), Co(II) and Fe(III) complexes

A new chelating resin, 1,8-(3,6-dithiaoctyl)-4-polyvinylbenzenesulphonate (dpvbs) has been synthesized by coupling Amberlite XAD-16 with (2,2′–ethylenedithio) diethanol using pyridine/CH2Cl2 mixture as a solvent. The chelating resin and its metallopolymer Cu(II), Ni(II), Co(II) and Fe(III) complexes have been synthesized and characterized by EDS, SEM, XPS, elemental analysis, spectral (IR, UV/Vis, EPR). The thermal analysis of the resin and its metallopolymer complexes indicated an endothermic spontaneous sorption mechanism with the liberation of water of hydration of the metal ions and that adsorbed by the free resin. At the solid liquid interface, the degrees of freedom increased during the sorption of the metal ions onto the resin. The surface area of polymer support and its metallopolymer complexes are estimated by (BJH) method. The batch equilibrium method was used for studying the metal sorption and selectivity at different pH values and different contact times at room temperature. ICP-AES was used to estimate the metal capacity of the resin for sorption of Cu(II), Ni(II), Co(II) and Fe(III) from aqueous solutions utilizing the batch equilibrium method. The sorption tendency of the metal ions by the resin was found to be: Cu(II) > Fe(III) > Co(II) > Ni(II). Adsorption kinetics was found to be fit the pseudo-second order model.

Influence of natural organic matter on the extraction efficiency of flame retardants from surface waters

The influence of natural organic matter (NOM) on the solid-phase extraction (SPE) efficiency was investigated for legacy and emerging flame retardants (FRs; n=26) in surface water. Three different groups of FRs were analyzed: polybrominated diphenyl ethers (PBDEs), halogenated flame retardants (HFRs), and organophosphorus flame retardants (OPFRs). In addition, five sorbents (Amberlite XAD-2, Amberlite IRA-743, Oasis HLB, Chromabond HR-P, and Chromabond HR-X) were evaluated for the extraction of FRs (n=33) in water, of which Oasis HLB eluted with dichloromethane and acetone:n-hexane (1:1, v/v) provided the highest overall recoveries. In subsequent NOM experiments, where FRs were extracted from water containing different NOM concentrations, both increased and decreased extraction efficiency with increasing NOM level were observed. Physicochemical and semi-empirical quantum chemistry properties were calculated for the FRs and used for analyzing relations between FRs. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) showed that the FRs separated into four different groups based on their properties. The FRs within each group responded similarly to increasing NOM, while differences in behavior were observed between the groups. This suggests that the structural properties of micropollutants highly influence NOM-FR interaction mechanisms. For instance, at high NOM levels, recoveries decreased substantially for FRs containing a moiety that can form strong hydrogen bonds (such as the double-bonded oxygen in e.g., OPFRs). Many of the compounds showed maximum extraction efficiency at higher levels of NOM. This suggests that binding of NOM to the sorbent and subsequent interaction between sorbent-bound NOM and FRs is an important mechanism for extraction of micropollutants from surface waters.

Ultrasonic intensification of sorption and desorption processes during the isolation of virginiamycin from the cultural broth of Streptomyces sp. S 15-30

&lt;p&gt;Supplementation of fermentation media with synthetic resins is used in the process of biotechnological production of antibiotics and other biologically active substances to prevent the auto-inhibition of the biosynthesis processes due to the binding of secreted target metabolites to a sorbent and their removal from the fermentation volume. The efficiency of a sorbent application may be improved via the ultrasonic stimulation of the antibiotic sorption from cultural broth during fermentation, and the similar stimulation of the reverse process (desorption) during its isolation and purification. In this study, the possibility of the use of ultrasound to improve the processes of sorption and desorption of virginiamycin, a feed antibiotic produced by the highly active strain&lt;em&gt; Streptomyces &lt;/em&gt;sp. S 15-30, to/from an Amberlite XAD-16 synthetic resin selectively binding this antibiotic, has been evaluated. According to the obtained results, the ultrasonic treatment with frequency of 22 kHz and acoustic energy density up to 0.05 W·s/cm&lt;sup&gt;3 &lt;/sup&gt;does not disrupt cell membranes, i.e., does not violate biotechnological processes. At the same time, such treatment increases the sorption capacity of the sorbent in ~1.4 times. The similar ultrasonic treatment of a sorbent at the acoustic field with energy density of 0.5 W·s/cm&lt;sup&gt;3&lt;/sup&gt; almost tenfold accelerates desorption of virginiamycin from the resin but does not destroy the sorbent and provides a possibility to its re-use after regeneration. The revealed effects of the ultrasonic treatment may be integrated into the technology of the virginiamycin biosynthesis, isolation, and purification to improve the efficiency of its industrial production.&lt;/p&gt;

Bio-butanol separation by adsorption on various materials: Assessment of isotherms and effects of other ABE-fermentation compounds

Abstract In the general effort to produce chemicals and fuels from renewable resources there is new interest in the biotechnological route to produce butanol. Acetone-Butanol-Ethanol (ABE) fermentation leads to low product yield because butanol is toxic to microorganisms. Selecting an efficient technology to recover butanol from low concentration butanol (about 2%) solutions is key to a successful implementation of this butanol production process on an industrial scale. Adsorption has been suggested as a potential technology for butanol recovery. This paper reports a systematic investigation of the adsorption of the metabolites that are typically present in the ABE fermentation broth: acetone, butanol, ethanol, acetic acid, butyric acid, yeast extract. The effect of glucose as a carbon source for the ABE fermentation was also investigated. Three adsorbents were characterized: Amberlite XAD-4, Amberlite XAD-7 and Zeolite Y. Batch tests under a wide interval of operating conditions were carried out for the assessment of the adsorption capacities of the three adsorbents. The selectivity of the three adsorbent materials towards the main components of ABE broths was also investigated. These tests were carried out using both dilute model solutions and real fermentation broths. The tests show that Amberlite XAD-7 has the highest affinity for butanol adsorption. The co-presence of all the metabolites in the solution - synthetic broth and real broth - changes the distribution in the adsorbed phase remarkably. The distribution of the concentration of the adsorbed components can be affected by pH. The presence of glucose does not affect butanol adsorption. Amberlite XAD-7 has a great potential as an adsorbent in ABE fermentation coupled with in situ recovery product removal techniques.

Differences between phenolic content and antioxidant capacity of quillay Chilean honeys and their separated phenolic extracts

The differences between entire quillay honeys and their separated phenolic extracts in terms of phenolic content, flavonoid content and scavenging activity assessed through an Oxygen Radicals Absorbance Capacity (ORAC) assay were determined. The separated phenolic extracts of honey were obtained using Amberlite XAD-2, which is the procedure most used in analytical methods, biological assays and functional food development. The results showed that phenolic and flavonoid contents and antioxidant activity were higher in entire honeys than in their separated phenolic extracts. The recovery of phenolic acids and flavonoids was variable and depended upon the method employed. The application of polyvinylpolypyrrolidone (PVPP), a water insoluble synthetic polymer, in the separated extracts indicated an important influence of non-phenolic reducing compounds in the polyphenolic content measured by the Folin–Ciocalteu method. This showed the elution with methanol of these compounds together with phenolic compounds using an Amberlite XAD-2 column. The antioxidant capacity assessed through ORAC-FL and ORAC-PGR was less influenced by the interference of non-phenolic compounds than by that of total phenolic and flavonoid contents.

Kinetic of carbonic anhydrase immobilized onto amberlite xad 7 and it application in sequestration of co2 into caco3 precipitate

Carbonic Anhydrase (CA) was immobilized onto Amberlite XAD 7 and was used in carbon dioxide sequestration purposes. The catalytic activities for free and immobilized CA were estimated by using para-Nitrophenyl Acetate (p-NPA) as the substrate in Tris-buffer containing 10% of acetonitrile. Lineweaver-Burk plot was employed to estimate the Michaelis-Menten kinetic parameters for both free and immobilized CA. Km value of free and immobilized CA were 2.92 mM and 5.7 mM respectively. Meanwhile the Vmax value of free and immobilized CA were 5.95 μmoles/min/ml and 2.67 μmoles/min/ml respectively. The kinetic value obtained in the present study shown that the immobilized CA has high affinity for its substrate. On the other hand, activity and stability study at various pH and temperature indicates that the optimum pH for free CA was found to be at pH 9 while for immobilized CA was at pH 10. For optimum temperature, a free CA was performed optimally at temperature 25°C and immobilized CA was working effectively at temperature 50°C. The immobilized CA onto Amberlite was tested in the CO2 sequestration process and the formation of the white CaCO3 precipitate was observed during the process. CaCO3 powder obtained during the process was validated with the XRD analysis. The finding indicated that immobilized CA onto Amberlite XAD7 retained it enzymatic activity and stability and thus perform well in the CO2 sequestration which gave the white CaCO3 precipitate.

Amberlite XAD Resins Impregnated with Ionic Liquids for Au(III) Recovery

Phosphonium‐based ionic liquids have been successfully impregnated on several Amberlite XAD resins for recovering Au(III) chloro‐anions. Sorption performance was evaluated through sorption isotherms (very favorable profiles) and uptake kinetics (controlled by the resistance to intraparticle diffusion). The binding mechanism consists of an ion‐exchange between AuCl4− and the counter ion bound to the phosphonium cation. Increasing the IL loading proportionally increases the sorption capacity but decreases the sorption speed (by increasing the resistance to intraparticle diffusion). Changing the counter ion on the phosphonium cation (i.e., complex anion vs. chloride ion) does not improve sorption performance. Highly porous resin (high pore volume and large pore size), such as Amberlite XAD‐1180, shows the best diffusion performance and highest sorption velocity (lower equilibrium time).

COMPOSITION OF PURIFIED ANTHOCYANIN ISOLATED FROM TEAK AND IT’S IN VITRO ANTIOXIDANT ACTIVITY

Objective: The present study evaluates purification, characterization of anthocyanin from in vitro culture of teak and its antioxidant potential.Methods: Anthocyanin was extracted from in vitro culture, purified by using amber lite XAD column and fractionated by Liquid chromatography mass spectrometry (LC-MS/MS). Various antioxidant assays were carried such as 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), 2,2'-azino-bis-3-ethyl-benzothiazoline-6-sulphonic acid (ABTS), Oxygen radical absorbance capacity (ORAC), Nitric oxide (NO) and Hydrogen peroxide (H2O2).Results: Liquid chromatography mass spectrometry (LC-MS/MS) revealed the major fraction as cyanidin 3-(2-xylosyl-rutinoside) with unknown peaks. The amount of anthocyanin was 15.23 mg/g monomeric anthocyanin. Further, the potential antioxidant capacity of the teak anthocyanin was comparable to common vegetables and fruits. Similarly, high correlations of anthocyanin with antioxidant activity, such as oxygen radical absorbance capacity (ORAC), 2,2'-azino-bis-3-ethyl-benzothiazoline-6-sulphonic acid (ABTS), and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) (r = 0.95, 0.93, and 0.80) were found.Conclusion: The high anthocyanins content and potential antioxidant activity suggests that teak anthocyanin may be applied in the food industry as a good source of natural pigments

Adsorption of Various Monoterpenoids on the Surface of Graphene and Nitrogen-Doped Graphene: A DFT Based Study

Monoterpenoids are widely distributed in nature and have been recognized to possess various pharmacological properties such as antifungal, antibacterial, antioxidant, anticancer, anti-spasmodic, etc. We analyzed the adsorptive interaction of certain monoterpenoids (such as carvacrol, carvone, γ-terpinene, limonene and menthol) with pristine graphene and nitrogen-doped graphene using DFT calculations. In addition, the adsorption properties are compared with two of the commercially available polymeric resins namely Amberlite XAD-4 and XAD-7. Full geometry optimization have been carried out in water medium at B3LYP and M06-2X levels. We observed that the level of the calculations does not affect the trend of the results and addition of dispersion interaction is important for getting meaningful energies. The basis set superposition errors (BSSE) are estimated by using counterpoise-correction method at M06-2X level. BSSE estimation lowers the interaction energies values but the overall trend is not affected with this estimation. The adsorption energy (▵Eads) is higher for parallel orientation of terpeniods to graphene and nitrogen-doped graphene surface compared to perpendicular orientation. In addition, ▵Eads values for carvacrol is highest and for menthol is lowest. Monoterpenoids absorbs more efficiently on the surface of nitrogen-doped graphene compared to graphene. This study offers an effective way to design promising adsorbent for selective separation of the terpenoids.

Applications of Amberlite XAD based Chelating resins in Online Pre concentration of metal ions

Applications of Amberlite XAD based Chelating resins in Online Pre concentration of metal ions

Separation and Pre-Concentration of Pb and Cd in Water Samples Using 3-(2-hydroxyphenyl)-1H-1,2,4-triazole-5(4H)-thione (HTT) and Their Determination by Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-AES)

A new method for the separation, pre-concentration and accurate determination of trace amounts of Pb and Cd in water samples using Amberlite XAD-16 resin functionalized with a new chelating ligand, 3-(2-hydroxyphenyl)-1H-1,2,4-triazole-5(4H)-thione (HTT), Amberlite XAD-16-HTT and inductively coupled plasma atomic emission spectrometry (ICP-AES) is reported in the present study. Fourier transform infrared (FTIR) spectroscopy was used to characterize the chelating resin. The effects of analytical parameters such as the pH of the medium, amount of adsorbent, type and volume of eluent, flow rate of the sample solution, volume of the sample solution, and matrix interference on the retention of metal ions were investigated. Also, 1 M HNO3 was used for the elution of the sorbed metals, and ICP-AES was used for the analysis of elutes offline. The results indicate that pH 5 is the optimum pH for the sorption of Pb and Cd ions. The limit of detection was found to be 0.16 and 0.22 μg/L for Pb and Cd, respectively, by applying a pre-concentration factor of 50. The method was validated using the international water reference material (NIST 1643e). The developed enrichment method has a high selectivity, sensitivity, and reproducibility; this method was successfully applied for the determination of Pb and Cd in surface water samples collected in Nellore District, Penner River belt as well as Bay of Bengal, Andhra Pradesh, India.