Amazon Region Research Articles

Tracking Boats on Amazon Rivers—A Case Study with the LoRa/LoRaWAN

The Amazon region has the largest hydrographic basin in the world. The rivers act as roads, and boats serve as vehicles for transporting passengers and cargo to large urban centers, municipalities, riverside communities, villages, and settlements. The Amazon River transportation system faces critical gaps due to the lack of land infrastructure in certain areas, which makes rivers essential for commerce and access to isolated communities. This work proposes the development of a new hardware platform consisting of a system-in-package (SiP)—iMCP HTLRBL32L and GPS, which enables data transfer over a network with long-range LoRa technology. In addition, we developed a new communication protocol between the end devices called the LoRa Protocol Proprietary (LPP). This protocol allows parameterizable commands (location table, modulation, routing, source and destination DevEUI, and port) to be sent between end devices, providing flexibility in the configuration and management of Internet of Things networks. The results of the practical experiments with the new hardware platform in the communication scenario between the end device and the gateway show that the maximum range was 16.928 km. In the communication scenario between the end devices, the maximum range was 12.447 km. It offers a stable platform for exchanging control information, which is fundamental to the safety of river transport in the Amazon.

Untargeted Characterization and Biological Activity of Amazonian Aqueous Stem Bark Extracts by Liquid and Gas Chromatography-Mass Spectrometry.

Aqueous stem bark extracts of Aspidosperma rigidum Rusby, Couroupita guianensis Aubl., Monteverdia laevis (Reissek) Biral, and Protium sagotianum Marchand have been reported as traditional remedies in several countries of the Amazonian region. Despite previous research, further investigation to characterize secondary metabolites and the biological activity of extracts is needed to derive potential applications. Metabolic profiling was carried out using liquid and gas chromatography coupled with mass spectrometry (UHPLC-MS/MS and GC-MS). The chemical composition of the studied plants was further compared by principal component analysis (PCA). Additionally, chemical profiles were correlated with antimicrobial and toxicity activities, which suggested potential metabolites for future research. We identified 16 and 32 compounds by UHPLC-MS/MS and GC-MS analysis, respectively. Antimicrobial activity was detected in three stem bark extracts. C. guianensis showed inhibition of all tested microorganisms, including antibiotic-resistant strains. Molecular networking approaches, in silico tools, and Pearson's correlation showed that antifungal compounds could be a terpene glycoside (r = 0.918) and/or a phenolic (r = 0.882) metabolite class. This study highlights the use of the established procedure in exploring the metabolomes of these species, which could be a novel source of antimicrobial drug discovery. Coupling the observed biological potential with UHPLC-MS/MS data has also accelerated the tracing of their bioactive compounds. These findings update the state of the art regarding the chemical composition and biological activity of the plant extracts, defining potential new applications for the pharmaceutical applications.

MEIO AMBIENTE E SUSTENTABILIDADE: ENSINO E POSTURA DE QUATRO IGREJAS CRISTÃS DE ABAETETUBA

In the last decades, there has been a strong concern on the part of the communities of our planet in relation to the environment, due to the changes caused by man in nature or even by the responses given by herself to the actions undertaken by him. This article analyzes the position of four Christian churches in relation to the environment and sustainability, on how religious institutions are promoting knowledge to understand environmental dilemmas, in addition to checking if there are any projects and / or guidance on these issues. The focus of the research was the municipality of Abaetetuba, an Amazon region where there are companies that wish to explore the locality. The analysis of this research will be done through graphics whose interviewees are members of these churches. It is concluded that the principles of Christian formation, religion as a link, establishes as a defining element the rules and guidelines that awaken in the faithful the awareness of rational and wise coexistence with nature, for the development of an environment conducive to quality of life, defining social well-being.

Monitoring of Selected Swine Viral Diseases in Peruvian Amazon Peccaries.

Peccaries (collared peccary-CP-and white-lipped peccary-WLP) are an essential source of protein and income for rural communities in the Amazon region. Since 1980s, researchers in the Amazon have reported recurrent local disappearances of WLP populations. Although such disappearances impact the species conservation and the food security of rural societies, no studies have drawn consistent conclusions about the causes of these population collapses. However, it has recently been proposed that the overabundance of this species before its decline would be related to infectious disease outbreaks. In the current study, we aimed to determine the circulation (occurrence and exposure) of viruses relevant to swine health in CP and WLP populations, namely classical swine fever virus (CSFV), Aujeszky's disease virus (ADV), swine vesicular disease virus (SVDV), and porcine circoviruses (PCV). The study was conducted in two areas of the northeastern Peruvian Amazon: the Yavarí-Mirín River basin (2008 -2020), where WLPs experienced extreme population fluctuations, and the Pucacuro National Reserve (2012-2014), where no WLP disappearances have been reported. Since WLP is not easily found during population declines, we also sampled CP as an indicator of virus circulation in the area as they are likely to be susceptible to the same pathogens. CSFV and ADV antibodies were detected in both peccary species and both areas. Diseases caused by CSFV and ADV have the potential to act as ultimate causes of population collapse, especially in large WLP populations where overabundance could increase the rate of pathogen transmission. Our results were inconclusive in establishing whether or not these viruses drove the WLP population to collapse, but their potential role warrants deeper investigation, expanding the geographical coverage of studies on infectious diseases in peccaries.

Human mobility and malaria risk in peri-urban and rural communities in the Peruvian Amazon.

While the global burden of malaria cases has decreased over the last two decades, the disease remains a major international threat, even on the rise in many regions. More than 85% of Peruvian malaria cases are in the Amazonian region of Loreto. Internal mobility primarily related to occupation is thought to be primarily responsible for maintaining endemicity and introducing and reintroducing malaria parasites into areas of anophelism, a challenge for malaria eradication. This study focuses on identifying the sources of malaria transmission and patterns of human mobility in order to understand the movement and transmission of the parasite. The assessment of connectivity produced by human mobility was evaluated in three districts of Loreto, through 10 cross-sectional population screening from 2018 to 2020. We used social network analysis (SNA) to obtain weighted and unweighted degrees of connectivity and explore its variability by socio-demographic characteristics. In addition, we integrated travel history and malaria incidence data to estimate parasite connectivity due to internal human mobility between locations. Finally, we used logistic multivariate regressions to explore the factors associated with Plasmodium spp. infection in mobile individuals. We found that internal human mobility results in high connectivity between communities from the Mazan, Iquitos, and San Juan Bautista districts. We identified nearby destinations that may act as sinks or sources for malaria transmission, including densely populated towns and rural campsites. In addition, we found that being a male, traveling to rural campsites, and working outdoors are associated with Plasmodium spp. infection in travelers from the Mazan district. We provide compelling evidence about how human mobility connects rural communities in the Peruvian Amazon. Using SNA, we uncovered district-specific patterns and destinations, providing further evidence of human mobility heterogeneity in the region. To address the challenge of human mobility and malaria in this setting, geographic heterogeneity of malaria transmission must be considered.

Modeling and advanced Ground Penetrating Radar techniques for evaluating pipe leakage and pavement distress

This study provides a comprehensive analysis of Ground Penetrating Radar (GPR) data processing techniques for detecting water leakage in urban pavements. By examining GPR images characterized by continuous and discontinuous high-amplitude reflections, the research identifies clear indicators of water saturation caused by leaking underground pipes. Computer modeling of GPR profiles enables effective comparisons with real data and helps in calibrating key GPR attributes such as energy, amplitude variance, and RMS amplitude. These attributes allow for the precise delineation of leakage zones in both synthetic and real GPR datasets collected in the Amazon region of Brazil. The Reflector Tilt Method (RTM) significantly enhances GPR data analysis, especially for detecting subsurface water leaks. RTM utilizes changes in the relative dielectric constant and electromagnetic wave velocity induced by water saturation in unconsolidated, horizontally stratified sand layers. By analyzing the tilt of reflections in GPR profiles, RTM differentiates between dry and saturated zones, providing insights into the geometry and extent of water infiltration. Notably, this study demonstrates how increased subsurface water saturation causes underlying reflectors to tilt. Using the tilt angle, we propose a novel technique to quantify the increase in the dielectric constant due to water saturation. This technique could also be adapted to detect leaks involving other fluids, as long as there is a strong contrast between the dielectric constant and the background. The integration of RTM improves the creation of accurate three-dimensional models of affected areas, enhancing leak detection and aiding in understanding water movement dynamics within the subsurface. The study underscores the importance of combining GPR attributes with RTM to generate detailed 3D models of leakage zones, which are essential for estimating the volume of infiltrated areas. Observations indicate significant water saturation near leaking pipes at shallow depths, with saturation levels decreasing rapidly as depth increases. By identifying 2D geophysical signatures of water leaks in public distribution networks, the research highlights the effectiveness of non-invasive GPR methods in mitigating risks such as soil erosion and surface collapse. This approach demonstrates the utility of GPR attributes and RTM as essential tools for assessing urban pavement integrity, enabling early detection of leaks and precise mapping of affected zones. The findings contribute significantly to the efficient and sustainable management of urban infrastructure, enhancing maintenance strategies and reducing potential hazards associated with undetected fluid leaks.

Discovering Accessible Financial Systems Resources for Emergent Users

The widespread use of mobile phones in Brazil has significantly altered information access and communication, impacting the financial sector as institutions increasingly use mobile apps. However, ensuring accessibility for all users remains challenging, particularly for riverside people who lack exposure to formal education, urban environments, and digital technologies. This study examined the accessibility and communicability barriers this population faces in mobile banking applications, especially in instant payment features. After reviewing best practices and applying the Semiotic Inspection Method (SIM), the study developed and tested an instant payment prototype with riverside people in the Amazon region. Interviews and the Communicability Evaluation Method (CEM) were used to measure communication effectiveness and inform design improvements for Pix interfaces. Afterward, a workshop with computing students was conducted, and they showed interest in inclusive systems but needed more practical knowledge. The workshop also highlighted the necessity of providing audio resources, more security, and illustrations and text that are easy to understand in financial conversation systems for emergent users. This research supports digital inclusion by exploring resources to improve accessibility in interactive systems for emergent users.

Well-managed grass is a key strategy for carbon storage and stabilization in anthropized Amazon soils.

Soils under anthropic use in the Amazon region are often associated with soil carbon (C) stock losses. More recently, the restoration of degraded pastures and the introduction of integrated systems have changed this pattern, and soil C accumulation is often observed. This study evaluated an 11-year field experiment to quantify soil C changes and elucidate C stabilization mechanisms in areas under anthropic uses in the southern Amazon of Brazil. Four land use systems were evaluated: crop succession (CS), integrated crop-livestock (ICL), integrated crop-livestock-forest (ICLF), and a well-managed pasture (MP). Land uses with a greater presence of well-managed grass increased soil C stocks, especially in the top 10cm, with values of 30.9, 29.7, 36.5, and 39.4Mgha-1 in the CS, ICLF, ICL, and MP systems, respectively. Compared to the baseline, ICL and MP systems showed soil C accumulation rates of 0.68-0.95MgC ha-1 yr-1. Greater aggregate stability and higher mineral-associated organic carbon (MAOC) were observed in both MP and ICL. X-ray photoelectron spectroscopy verified 11%, 38%, and 32% more recalcitrant C groups (aliphatic/aromatic) in the MP system than ICL, ICLF, and CS at 0-5cm. In the ICLF system, the eucalyptus row showed 15% lower soil C stocks, less MAOC, and less abundance of recalcitrant groups than the inter-row position. Land use systems with long-term spatial-temporal use of grass in well-managed pastures or ICL promoted greater C stabilization through intra-aggregate occlusion, mineral sorption, and chemical recalcitrance, representing a good strategy to enhance C storage in Amazon anthropized soils.

Pretreatment of Oryza sativa (Rice) and Musa cavendishii (Banana) Waste Biomass Using Ionic Liquids of Choline Amino Acid for Nanoscale Cellulose Production

The species Oryza sativa (rice) and Musa cavendishii (banana) are sources of cellulose-rich waste biomass in the Amazon region, Tocantins State. This research investigates the nanoscale production of cellulose through the interactions between three choline amino acid ionic liquids Ch[AA]IL and the respective fibers by pretreatment. To this end, the synthesis of three ILs was carried out: choline arginate Ch[Arg], choline glycinate Ch[Gly] and choline lysinate Ch[Lys], characterized by Fourier transform infrared spectroscopy (FTIR). The samples resulting from the pretreatment were analyzed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). It was possible to infer from the SEM micrographs that Ch[Arg] caused greater fiber breakage than the other ILs. The TEM analyses identified fibers up to 16 nm in diameter. Positive effects were observed in the diffractograms, although no crystallinity was obtained in the pretreated samples. Thermogravimetry curves showed that the fibers treated with Ch[Arg] showed higher thermal stability.

Towards culturally inclusive healthcare in Peru: Mapping epistemic concepts in contemporary Indigenous Amazonian medicine-Traditional healers' perspectives.

Peru is among Latin American countries with the largest Indigenous population, yet ethnical health disparities persist, particularly in the Amazon region which comprises 60% of the national territory. Healthcare models that include Indigenous medicine and traditional healers present an important avenue for addressing such inequalities, as they increase cultural adequacy of services, healthcare access, and acknowledge Indigenous Rights for their perspectives to be represented in public healthcare. Understanding the underlying epistemologies of Indigenous medicine is a prerequisite for this purpose. Thus, in order to support Indigenous Organizations and governmental initiatives to develop more inclusive healthcare approaches, the current study investigated key epistemic concepts in Indigenous-Amazonian medicine from the perspective of traditional healers. We conducted systematic in-depth interviews (semi-structured) with a sample of 13 healers of three Peruvian-Amazonian regions (Loreto, Ucayali, San Martín). Data was analysed using manifest qualitative content analysis. Our findings point to an intricate medical system based on a sophisticated understanding of health, illness, and treatment. Indigenous healers described multifactorial aetiology concepts, complex interactions between material and spirit-related aspects of body and nature, diagnosis, and treatment. These often involved carefully designed applications of 'teacher plants', a concept at the heart of this medical system. Furthermore, while the healers considered traditional and biomedicine as complementary systems, they identified the lack of recognition of traditional healers as a primary barrier for collaboration. Indeed, preconceptions and stigma on Indigenous medicine along with a paucity of research, still represent an impediment to countries' ability to respond to Indigenous peoples' health-related expectations and needs, thus maintaining existing inequalities. This work offers a significant contribution to the understanding of Indigenous-Amazonian medicine and perspectives of traditional healers, relevant for Peru and adjacent countries sharing Amazonian territory and cultures. Our findings also highlight Amazonian healers' unique expertise around the therapeutic applications of psychoactives, from which the current revival of clinical scientific interest in psychedelic-assisted therapies may have a great deal to learn.

Childbirth dynamics in the riverside region of the Brazilian Amazon from the perspective of geospatialization.

to analyze the spatial-temporal pattern of childbirths and flow of postpartum women assisted at a regional reference maternity hospital. ecological study of 4,081 childbirths, between September 2018 and December 2021, at a public maternity hospital in the Baixo Tocantins region, Pará, Brazil. With data collected from five sources, a geographic database was constructed, and spatial analysis was used with Kernel density interpolator. Maps were generated using QGis/3.5 and TerraView/4.3, calculating chi-square (p<0.05). the highest concentrations of normal and cesarean childbirths were observed in Barcarena (n=2,558/62.68%), Abaetetuba (n=750/18.38%), Moju (n=363/8.89%) and Igarapé-Miri (n=219/5.37%). Among the municipalities in the region, ten had obstetric beds, totaling 210 beds. In this scenario, postpartum women traveled up to 288 km to reach the maternity hospital. long distances between certain municipalities of residence and maternity hospital, and low supply of obstetric beds, were identified as risk factors for unfavorable obstetric outcomes.

Workers’ perception of solid waste management in higher education institutions in the Amazon region of Brazil

Workers’ perception of solid waste management in higher education institutions in the Amazon region of Brazil

Analysis of eco-friendly business practices and their impact on environmental sustainability in a Peruvian Amazon region

Eco-friendly business practices range from energy efficiency to sustainable management; therefore, the implementation of these practices not only has the potential to mitigate environmental impacts, but also to improve the long-term competitiveness and sustainability of companies. In this study we analyze eco-friendly business practices and their impact on environmental sustainability in companies in a region of the Peruvian Amazon. A quantitative, non-experimental approach was considered, with an explanatory design; for which, a sample size of 200 companies was considered, which were randomly selected, to which two instruments were applied, the first with 7 indicators and the second with 3, valid, with an alpha of 0.720 and 0.670 respectively, assessing structural equation modeling. The results show that eco-friendly business practices are oriented towards sustainability (C.R. = 19.280), as well as preventive and eco-efficient practices (C.R. = 5.023, C.R.= 14.185); these findings can serve as a basis for the creation or improvement of public policies that promote eco-friendly business practices, encouraging companies to adopt measures that favor environmental sustainability. Therefore, eco-friendly business practices have a positive impact on environmental sustainability in companies in a region of the Peruvian Amazon; in addition, preventive practices and eco-efficient practices have a positive impact on environmental sustainability in companies in a region of the Peruvian Amazon.

Regional development and transport infrastructure in the Brazilian amazon region: a literature review on governmental plans for the Transamazon Highway

Regional development and transport infrastructure in the Brazilian amazon region: a literature review on governmental plans for the Transamazon Highway

Clinical and epidemiological characterization of patients with cutaneous tuberculosis treated at a referral center in the Brazilian Amazon: case series.

Cutaneous tuberculosis is a rare form of the disease that defies diagnosis due to the diversity of clinical presentations. This study was based on the reality of a dermatology referral center in the Brazilian Amazon region to detail several characteristics of this disease. To describe a series of cases of cutaneous tuberculosis treated at a dermatology service in the Brazilian Amazon region, addressing epidemiological aspects, clinical forms, diagnostic methods, treatment, and outcomes. This is a descriptive and observational study of the case series type, including eight patients with a confirmed diagnosis of cutaneous tuberculosis seen between 2021 and 2023. A standardized protocol was used to collect data from patients' medical records. Among the eight cases of cutaneous tuberculosis, there were four cases of erythema induratum of Bazin, one case of tuberculosis verrucosa cutis, two cases of scrofuloderma, and one case of lupus vulgaris. Erythema induratum of Bazin, the most common form in the study, occurred predominantly in women, presenting as infiltrated plaques and erythematous nodules. All eight cases were treated with the standard therapeutic regimen of the Brazilian Ministry of Health (rifampicin, isoniazid, pyrazinamide, and ethambutol). They include the small sample size, making generalizations difficult. The study addressed the clinical diversity of cutaneous tuberculosis, with erythema induratum of Bazin as the most common form. The importance of the socioeconomic context in the prevalence of the disease and the need for more comprehensive epidemiological studies to improve the understanding of cutaneous tuberculosis, especially in endemic regions, are highlighted.

Gut bacterial communities in roadkill animals: A pioneering study of two species in the Amazon region in Ecuador.

Studying the microbial communities within the gastrointestinal tract of vertebrate species can provide insights into biodiversity, disease ecology, and conservation. Currently, we have very limited understanding of the composition of endogenous microbiota in wildlife, particularly in high biodiversity tropical areas. Knowledge is limited by the logistical and ethical challenges of obtaining samples for free-living animals. Roadkill carcasses offer a largely untapped source for biological material, including endogenous gut microbiota. These animals that have died on roads due to collisions with vehicles are suitable for accessible, opportunistic sampling. Here, we used metabarcoding for the V3-V4 region of the 16S rRNA gene in gut samples of nine roadkill samples collected from a road in Ecuador representing two vertebrate species: the speckled worm lizard (Amphisbaena bassleri) and the smooth-billed ani (Crotophaga ani). We successfully identify microbial phyla in both samples including Firmicutes, Bacteroidetes, and Proteobacteria for A. bassleri, and Firmicutes and Actinobacteria for C. ani. Our study provides the first description of the gut microbiota for these two vertebrates, and demonstrates the feasibility of studying endogenous microbial communities from roadkill material that can be opportunistically collected and preserved in biobanks.

Original and foreign languages in Pedagogical Political Projects in undergraduate courses: focus on a border region in the Brazilian Amazon

Evidenciando a importância de se discutir sobre o perfil profissional dos docentes de línguas nas Amazônias brasileiras, neste artigo, busca-se avaliar de que modo se contemplam, em Projetos Pedagógicos Curriculares, as línguas estrangeiras e as línguas faladas[1] por povos indígenas, além de questões histórico-culturais que influenciam na formação de professoras e professores dos Cursos de Licenciaturas em Letras da Universidade Federal do Acre (UFAC). Enquanto ao método, optou-se por uma abordagem qualitativa mediante estudo bibliográfico para se analisar de que modo esses documentos atendem a uma formação orientada à diversidade pluricultural, com base no aporte teórico da Linguística Aplicada trans/indisciplinar. Os resultados evidenciam que os componentes curriculares desenvolvidos nos cursos de licenciatura em Letras da UFAC contemplam, de maneira geral e específica, as temáticas referentes às linguagens, culturas e sociabilidades indígenas, negras e afrobrasileiras, o que, de certa forma, pode dar subsídio para futuros professores discutir questões referentes a problemas linguísticos e raciais em sala de aula, tornando os espaços educacionais mais plurais e inclusivos. Não obstante, evidencia-se a escassez de componentes orientados ao ensino de outras línguas estrangeiras distintas daquelas objeto de estudo, dado que sinaliza fragilidade nos Projetos Políticos Pedagógicos, e que dificulta, portanto, a formação plurilingue dos futuros professores. [1] Utilizamos a forma generalista “língua falada” sem desconsiderar as especificidades e importância de línguas sinalizadas.

Integrative use of DNA barcode and morphology reveal high level of diversity in the ornamental fish on the lower Amazon basin.

The Amazon basin is the world's largest hydrographic basin, in terms of both its total area and its species diversity, with more than 2,700 species of fish. Despite this diversity, the data available on the fish fauna of the Amazon basin are still relatively scant and incomplete, in particular from the streams and floodplain lakes of the lower Amazon, which may contain a large proportion of the still undescribed species of the basin. Many of these species are expected to be of interest to the ornamental fish market. The investigation of the diversity of potential ornamental fish using molecular tools is even more limited. Given this scenario, the present study employed DNA barcoding to investigate the diversity of ornamental fish found in two streams and a floodplain lake of the lower Amazon. The mitochondrially encoded cytochrome c oxidase I (MT-CO1) molecular marker was used to identify the taxa, in combination with morphological keys. A total of 51 ornamental species were identified, representing 13 families and three orders. A majority of the species were found at only one of the sampling points, which indicates that the distribution of the species is influenced by ecological factors. The most speciose order was the Characiformes, followed by the Cichliformes and Siluriformes, while the family with the greatest diversity of species was the Acestrorhamphidae (31.3% of the total number of species), followed by the Cichlidae (27.4%), and the Lebiasinidae (9.8%). One specie was registered in the region of the lower Amazon for the first time, and evidence was found of the possible existence of species not formally described of Aphyocharax, Astyanax, Apareiodon and Hemigrammus.

Sustainable Economic Growth and Land Management: A Case Study on the Role of Tax Legislation in Emerging Markets

The purpose of this study is to examine how tax incentives resulting from the so-called Amazon Law (Law No. 27037) affect small- and medium-sized agro-industrial producers (SMEAPs) in the Junín and Huánuco regions in Peru. This research fills a void that relates to the exclusion of these producers regarding the Law’s incentives that aim to encourage investment in the Amazon. In this study, the research design was non-experimental, and since the data were descriptive–correlational in nature, a structured questionnaire with a Likert scale was used to gauge participants’ opinions about economic progress and tax benefits. The survey participants included 72 co-operatives drawn from a population of 88, and their awareness and use of tax incentives were targeted. SPSS and similar statistical analysis tools were used and showed that there was a positive correlation between tax benefits and economic development, with a correlation coefficient of 0.873, indicating a strong relationship. However, most co-operatives ranked the benefits only as average or poor, with 34.72% rating them as regular and 31.94% as poor. This study indicates that the present laws do not provide these producers with sufficient opportunities for development. The authors suggest that changes to the Law are required to improve the inclusion of small- and medium-sized agricultural producers so that proposals for improvements in their economic development and management of the agricultural lands in the Amazon region can be promoted.

Soil pH modulates the activity of low-affinity methane oxidation in soils from the Amazon region.

In the Amazon region, pastures are the main land use subsequent to deforestation and this change can result in soil acidification and degradation. Liming is a management practice to increase soil pH, important to recover degraded lands and increase soil fertility, but its impacts on soil methane cycling in tropical soils are unknown. Here we investigate the role of soil pH on methane uptake under high concentrations of the gas, manipulating pasture and forest soils pH by liming and evaluating the active methane cycling microbial community. Top layer of forest and pasture soils were subjected to liming treatment and incubated with ∼10 000ppm of 13CH4. Soil DNA was evaluated with Stable Isotopic Probing (SIP-DNA), methanotrophic abundance was quantified (pmoA gene), and high throughput sequencing of 16S rRNA was performed. Liming increased the methane uptake in both forest (∼10%) and pasture (∼25%) soils. Methanotrophs Methylocaldum spp. (type I) and potential methanotrophs in Beijerinckiaceae (type II) were identified to actively incorporate carbon from methane in limed pasture soils. In limed forest soils, Nitrososphaeraceae were identified as 13C-enriched taxa, indicating that ammonia oxidizers can oxidize methane in these soils. Liming Amazonian pasture soils not only contributes to the fertility and recovery of degraded areas but also has the potential to improve the oxidation of methane at high concentrations of this gas.