Alternative Production Systems Research Articles

Overexpression of tryptophan decarboxylase and strictosidine synthase enhanced terpenoid indole alkaloid pathway activity and antineoplastic vinblastine biosynthesis in Catharanthus roseus.

Terpenoid indole alkaloid (TIA) biosynthetic pathway of Catharanthus roseus possesses the major attention in current metabolic engineering efforts being the sole source of highly expensive antineoplastic molecules vinblastine and vincristine. The entire TIA pathway is fairly known at biochemical and genetic levels except the pathway steps leading to biosynthesis of catharanthine and tabersonine. To increase the in-planta yield of these antineoplastic metabolites for the pharmaceutical and drug industry, extensive plant tissue culture-based studies were performed to provide alternative production systems. However, the strict spatiotemporal developmental regulation of TIA biosynthesis has restricted the utility of these cultures for large-scale production. Therefore, the present study was performed to enhance the metabolic flux of TIA pathway towards the biosynthesis of vinblastine by overexpressing two upstream TIA pathway genes, tryptophan decarboxylase (CrTDC) and strictosidine synthase (CrSTR), at whole plant levels in C. roseus. Whole plant transgenic of C. roseus was developed using Agrobacterium tumefaciens LBA1119 strain having CrTDC and CrSTR gene cassette. Developed transgenic lines demonstrated up to twofold enhanced total alkaloid production with maximum ninefold increase in vindoline and catharanthine, and fivefold increased vinblastine production. These lines recorded a maximum of 38-fold and 65-fold enhanced transcript levels of CrTDC and CrSTR genes, respectively.

Risks and opportunities of increasing yields in organic farming. A review

Current organic agriculture performs well in several sustainability domains, like animal welfare, farm profitability and low pesticide use, but yields are commonly lower than in conventional farming. There is now a re-vitalized interest in increasing yields in organic agriculture to provide more organic food for a growing, more affluent population and reduce negative impacts per unit produced. However, past yield increases have been accompanied by several negative side-effects. Here, we review risks and opportunities related to a broad range of sustainability domains associated with increasing yields in organic agriculture in the Northern European context. We identify increased N input, weed, disease and pest control, improved livestock feeding, breeding for higher yields and reduced losses as the main measures for yield increases. We review the implications of their implementation for biodiversity, greenhouse gas emissions, nutrient losses, soil fertility, animal health and welfare, human nutrition and health and farm profitability. Our findings from this first-of-its-kind integrated analysis reveal which strategies for increasing yields are unlikely to produce negative side-effects and therefore should be a high priority, and which strategies need to be implemented with great attention to trade-offs. For example, increased N inputs in cropping carry many risks and few opportunities, whereas there are many risk-free opportunities for improved pest control through the management of ecosystem services. For most yield increasing strategies, both risks and opportunities arise, and the actual effect depends on management including active mitigation of side-effects. Our review shows that, to be a driving force for increased food system sustainability, organic agriculture may need to reconsider certain fundamental principles. Novel plant nutrient sources, including increased nutrient recycling in society, and in some cases mineral nitrogen fertilisers from renewable sources, and truly alternative animal production systems may need to be developed and accepted.

Helminth infections in chickens (Gallus domesticus) raised in different production systems in Brazil.

The current study evaluated helminth infections in birds raised in different production systems for different purposes (extensive/dual-purpose, semi-intensive/broiler, semi-intensive/hen, intensive/hen and intensive/broiler) in Brazil. A total of 374 birds was assessed for helminths at necropsy using standard parasitological methods. During the necropsies, organs from the gastrointestinal tract (crop, esophagus, proventriculus, gizzard, small intestine, large intestine and ceca) of each bird were collected and the contents fixed in 70% ethanol. Additionally, the trachea and eyes were assessed for the presence of helminths. The small intestine was examined using a methodology that allowed the recovery of cestode scolices attached to the intestinal mucosa. Stereomicroscopy and optical microscopy were used to detect and identify helminth species based on their morphological characteristics. Fifteen helminth species were found among birds from the different systems. The extensive system presented the highest number of helminth species (six cestodes, seven nematodes and one trematode) and the highest number of parasites (mean helminths/bird), followed by the semi-intensive system (broiler: six cestode and four nematode species; hens: five cestode and three nematode species). Hens from the intensive system were parasitized by five cestode, four nematode and one trematode species. No parasites were detected in broilers raised in the intensive systems. The results obtained in this study highlight the need for special attention and the implementation of biosecurity measures for the prevention of helminth infections in intensive systems (hens) and particularly in extensive and semi-intensive alternative poultry production systems.

Neoliberalism and the Emergence of Private Sustainability Initiatives: The Case of the Brazilian Cattle Value Chain

AbstractRecent studies have examined the political significance of the emergence of governmental initiatives to develop sustainability standards for agricultural commodities in the transition economies. Yet, few studies have examined how neoliberal ideas influence the governance of value chains in the agri‐food sector of these countries. This paper's goal is to explore the changing role of the state in sustainable cattle value chain governance in Brazil, through a methodology of participant observation of the Brazilian Roundtable on Sustainable Livestock (GTPS). The results describe the technologies used to govern the cattle value chain, which involves a private action controlled at a distance by the state that is aimed at making products acceptable on world markets. This neoliberal governmentality does not address some crucial sustainability issues of the cattle value chain. The findings call for an extension of evaluation methods so that more attention can be devoted to alternative cattle production systems. Copyright © 2018 John Wiley & Sons, Ltd and ERP Environment

Transient Expression and Purification of Horseradish Peroxidase C in Nicotiana benthamiana.

Horseradish peroxidase (HRP) is a commercially important reagent enzyme used in molecular biology and in the diagnostic product industry. It is typically purified from the roots of the horseradish (Armoracia rusticana); however, this crop is only available seasonally, yields are variable and often low, and the product is a mixture of isoenzymes. Engineering high-level expression in transiently transformed tobacco may offer a solution to these problems. In this study, a synthetic Nicotiana benthamiana codon-adapted full-length HRP isoenzyme gene as well as C-terminally truncated and both N- and C-terminally truncated versions of the HRP C gene were synthesized, and their expression in N. benthamiana was evaluated using an Agrobacterium tumefaciens-mediated transient expression system. The influence on HRP C expression levels of co-infiltration with a silencing suppressor (NSs) construct was also evaluated. Highest HRP C levels were consistently obtained using either the full length or C-terminally truncated HRP C constructs. HRP C purification by ion exchange chromatography gave an overall yield of 54% with a Reinheitszahl value of >3 and a specific activity of 458 U/mg. The high level of HRP C production in N. benthamiana in just five days offers an alternative, viable, and scalable system for production of this commercially significant enzyme.

Design and Testing of a Modified Hydroponic Shipping Container System for Urban Food Production

In urban centers today, Controlled Environment Agriculture is being proposed as a potential alternative to conventional agriculture using hydroponic methods in controlled spaces as a means to increase local food production and improve urban food security by growing crops. One newly proposed technique, growing crops inside refurbished shipping containers, offers a flexible, mobile, and scalable means of year-round food production in a variety of climates. Despite the benefits of producing food locally, some concerns associated with shipping container systems include high-energy consumption from climate control and electric lightning systems, as well as expensive capital investments. This study investigated the viability and effectiveness of shipping container farms as alternative food production systems through an analysis of system energy requirements and resulting crop yields. A Modified Hydroponic Shipping Container system was designed and a Nutrient Film Technique hydroponics system was tested by growing lettuce plants and monitoring energy use throughout the growth period. Theoretical energy use at full scale was quantified for one year of production by modeling energy consumption of major system components through modeling or extension from results on the bench scale. Baseline crop production and overall energy consumption were assessed using a crop production efficiency metric created to evaluate the ratio of system outputs to inputs. Examination of alternative energy scenarios showed potential energy consumption reductions of up to 53 percent and an improvement of the total system crop production efficiency of up to 55 percent from the baseline. Implementation of suggested energy use reduction strategies could allow for the creation of viable and sustainable alternative food systems using shipping containers capable of providing local, accessible foods year-round for a variety of urban communities.

An Ecosophical Inquiry into Digital Mediation and Design in Relation to Alternative Food Networks (AFNs) in an ‘Expanded Field’ of ‘Agri-culture’

T HIS paper brings together several fields of inquiry, including agriculture, art, digital media, design and philosophy, to make a tentative, exploratory re-framing of Alternative Food Networks ( AFN s), and so to re-think and catalyse fresh opportunities for investigation in the humanities. In order to understand the ‘now’, 1 we have to begin with the long view of the agricultural story and, critically, the mediation of this story. We need to understand how sequential economic, socio-technical and other factors encouraged paradigmatic shifts in the ways we produce, consume and perceive our food. Since Medieval times farmers and producers have been testing new (alternative) systems of production, variously encouraged or resisted by the dominant socio- technical regime. 2 Reaction to the total dominance of agri-industrial farming in the industrialised, northern, western world nations since the 1950s and now globally led to early experiments in different producer-consumer relations, for example through Community Supported Agriculture ( CSA ) schemes, from the 1980s onwards. 3 During the last twenty years these experiments have multiplied under various monikers, such as sustainable farming, bio-farming, organic farming and AFN s to such an extent that they now represent an ‘expanded field’, to borrow a concept from the art critic Rosalind Krauss. 4 Applying this notion of the expanded field to agriculture leads to a clarification of the terminology and diversity of initiatives that contest intensive agri-industrial farming. Furthermore, it suggests that we are actually exploring a phenomenon of agri-culture, not agriculture, that is the development of farming and food system practices underpinned by new cultural practices and values. 5 Zooming in on the AFNs, which focus on developing new producer-consumer relations, it seems appropriate to consider these developments within Felix Guattari’s ecosophy 6 which he proposed as a means to counter the hegemony of Integrated World Capitalism (IWC), or as we know it today, neo-liberal, global capitalism. So, the key questions raised here are how do the AFNs choose to mediate their activities online, whether they embed an ecosophical approach in doing so, and how these might represent new agriculture. Lastly, I examine the potentiality of design to contribute to amplifying and scaling up AFNs and other practices in ‘an expanded field of agri-culture’.

Evaluation of gas turbines as alternative energy production systems for a large cruise ship to meet new maritime regulations

As a consequence of the new and up-coming regulations imposed by the International Maritime Organization (IMO), polluting emissions produced by large ships are now under strict control. Moreover, specific areas called “Emission Controlled Area” (ECA), which request even lower pollutant emissions, will be extended.To face up to this issue, ships propelled by Internal Combustion Engines (ICEs) burning Heavy Fuel Oil (HFO) can be equipped with abatement devices such as scrubbers and Selective Catalytic Reactor systems. Along with these solutions, which seem to be the route ship-owners will prefer, other methods can be considered, such as the use of Marine Gas Oil (MGO): a more expensive fuel, but with lower sulphur content. The use of MGO allows users to consider a further and more drastic modification of the power system, namely the use of Gas Turbines (GTs) in place of ICEs. GTs, despite being less efficient, are much lighter, more compact, and can more easily reach low NOx emissions than ICEs. Even if these aspects are theoretically well known, there are still difficulties in finding studies reporting quantitative analysis (weight, dimensions, fuel consumption) that compare GT and ICE power systems employed on board.The present paper aims to provide these data by analyzing different solutions applied to a real case. Unlike other studies, the work is focused on a cruise ship rather than on a cargo ship, because a cruise ship’s operation profile is more variable during the trip.

Ecoservices and multifunctional landscapes: Balancing the benefits of integrated ES-based water resources, agricultural and forestry production systems

Ecosystem services (ES) are a powerful tool in assessing the relationships that people have with their surrounding tangible environment and the more distant intangible global benefits. Ecoservices are often studied in parallel or viewed as linear, and this misunderstanding or ignorance of the complex interaction among ecoservices may lead to poor or inappropriate land and water management decisions. Quantitatively understanding and establishing the risk to the complex functional relationships among ES impacted by the introduction of alternate production systems requires a new pathway to better decision making.Ecoservices are often interrelated in dynamic and complex ways and provide an avenue, if appropriately coupled, to a more balanced land use-land cover (LULC) multifunctional production system. Variable land use intensities and landscape configurations impact differentially on selected ecosystem functions and services. Anthropogenic activities impact this functionality and require an innovative analytical approach to building an integrated multifunctional ES analytical framework (IMESAF); an approach that spans diverse fields of inquiry.An IMESAF would need to incorporate a variety of ecoservices-based best practice production systems that can elucidate balanced land use scenarios to deliver better environmental and productivity outcomes. In addition, this framework requires policy, economic, sustainability, monitoring and evaluation indices to provide guidance on intermediate and long-term effects of an ecoservices-based production and planning system. This paper provides a synthesis of ideas for creating the framework and how it may underpin balanced land use and investment choices.

Expression and purification of the extracellular domains of human glycoprotein VI (GPVI) and the receptor for advanced glycation end products (RAGE) from Rattus norvegicus in Leishmania tarentolae

ABSTRACTGlycosylation is one of the most complex post-translational modifications and may have significant influence on the proper function of the corresponding proteins. Bacteria and yeast are, because of easy handling and cost reasons, the most frequently used systems for recombinant protein expression. Bacteria generally do not glycosylate proteins and yeast might tend to hyperglycosylate. Insect cell- and mammalian cell-based expression systems are able to produce complex N-glycosylation structures but are more complex to handle and more expensive. The nonpathogenic protozoa Leishmania tarentolae is an easy-to-handle alternative expression system for production of proteins requiring the eukaryotic protein folding machinery and post-translational modifications. We used and evaluated the system for the secretory expression of extracellular domains from human glycoprotein VI and the receptor for advanced glycation end products from rat. Both proteins were well expressed and homogeneously glycosylated. Analysis of the glycosylation pattern identified the structure as the conserved core pentasaccharide Man3GlcNac2.

Animal performance and economic implications of alternative production systems for dairy bulls slaughtered at 15 months of age

AbstractThe objectives of this experiment were to investigate (i) the influence of varying levels of concentrate supplementation during the grazing season, (ii) alternative finishing strategies for dairy bulls slaughtered at 15 mo of age and (iii) economic implications of these management strategies. Bulls were assigned to a 2 (level of concentrate supplementation during the grazing season: 1 kg [LA] and 2 kg [HA] dry matter [DM]/head daily) × 2 (finishing strategies: concentrates ad libitum group [AL] or grass silage ad libitum plus 5 kg DM of concentrates/head daily group [SC]) factorial arrangement of treatments. Average daily gain (ADG) during the grazing season was greater (P < 0.01) for HA than for LA. Consequently, HA bulls were 16 kg heavier at housing: 214 and 230 kg, respectively (P < 0.05). During the finishing period, ADG tended (P = 0.09) to be greater for LA than for HA. Carcass weight tended (P = 0.08) to be greater for HA than for LA. Fat score was greater for HA. Live weight at slaughter (P < 0.001) and carcass weight (P < 0.001) were 41 and 23 kg greater for AL than for SC, respectively. Conformation (P < 0.05) and fat score (P < 0.05) were greater for AL than for SC. The Grange Dairy Beef Systems Model simulated whole-farm system effects of the production systems. Net margin/head was greater for LA than for HA and greater for SC than for AL. Sensitivity analysis of finishing concentrate price, calf purchase price and beef price showed no re-ranking of the systems on a net margin basis. Although greater animal performance was observed from the higher plane of nutrition, overall profitability was lower.

DISPOSICIÓN DE PLANTAS DE TOMATE EN DOSELES EN FORMA DE ESCALERA BAJO DOS DENSIDADES DE POBLACIÓN

El manejo de la arquitectura de la planta es de suma importancia en sistemas de producción intensiva. El objetivo del presente estudio fue evaluar el crecimiento y rendimiento de plantas de tomate (Solanum lycopersicum L.) bajo un sistema de producción alternativo basado en el despunte temprano de las yemas terminales para dejar tres racimos por planta y alta densidad de población, en condiciones hidropónicas y bajo invernadero. Se compararon tres disposiciones de plantas con doseles en forma de escalera (escaleriformes) y una cuarta con dosel uniforme (testigo). Se usaron plantas de tomate Pik Ripe 461 de hábito de crecimiento determinado. Dentro de cada sistema de producción se probaron dos densidades de población (10 y 12.5 plantas m-2). Se utilizó un diseño de parcelas divididas en bloques al azar con cuatro repeticiones. En las parcelas grandes se evaluaron los sistemas de producción y en las subparcelas las densidades de población. Se analizaron características morfológicas y variables de rendimiento. Se encontró que las plantas con arreglos de dosel escaleriforme rindieron entre 18 y 21 % más por unidad de superficie que las de dosel uniforme, debido a que produjeron mayor cantidad de frutos sin una disminución significativa de su peso medio. La densidad de población con que se logró el mayor rendimiento (31 kg m-2) fue de 12.5 plantas m-2. El sistema de manejo propuesto fue efectivo para incrementar el rendimiento de tomate por unidad de superficie bajo condiciones de invernadero.

Nest use and patterns of egg laying and damage by 4 strains of laying hens in an aviary system1

Laying hens are strongly motivated to use nests for egg laying, and alternative production systems (e.g., aviaries) provide artificial sites to meet this need and ensure efficient collection of clean, undamaged eggs. However, nests are typically not provided to allow simultaneous use by all hens; therefore, competition or mislaid eggs can result. To understand the influence of strain on laying eggs outside nests and damage to eggs, we compared daily patterns of nests use and egg laying among 4 laying hen strains (Hy-Line Brown (HB), Bovans Brown (BB), DeKalb White (DW), and Hy-Line W36 (W36)). Hens were observed over 3 consecutive days in aviaries with colony nests in the enclosure’s top tier (2 nests/unit, 4 aviary units/strain, 144 hens/unit). The number and location of hens in nests and the number, location and condition of eggs throughout aviaries were recorded. Most eggs (90 to 95%) were laid in nests; however, brown hens consistently laid more non-nest eggs and damaged more eggs than white hens (P ≤ 0.05). Higher nest occupancy by brown hens was correlated with more non-nest and damaged eggs (P ≤ 0.05). In the morning, brown hens occupied more nest space and laid more nest eggs than white hens (e.g., HB vs. DW: 82.97 and 34.66% of space; 91.35 and 68.73% of nest eggs; P ≤ 0.05). At midday, white hens occupied more nest space and laid more nest eggs than brown hens (e.g., HB vs. DW: 28.47 and 15.81% of space; 27.39 and 8.29% of nest eggs; P ≤ 0.05). Brown hens preferred right nest compartments and laid more eggs there, whereas white hens preferred left compartments and W36 laid more eggs there (P ≤ 0.05). These findings indicate that different strains of hens have different patterns of nest use and laying behavior. In brown hens, heavy morning nest use was related to laying eggs outside nests and more damaged eggs, suggesting insufficient space for oviposition in nests. Specific facility design should be matched to hens’ preferences to accommodate behavioral needs of different strains.

Biochar addition in rice farming systems: Economic and energy benefits

This study investigated economic returns and energy use of alternative rice production systems in North Vietnam with various residue management options. The traditional practice of open burning of rice residues (System A) was compared with the alternative of converting residues to biochar, which was returned to the paddy fields (System B). It was assumed that households used improved cook-stoves and drum ovens to produce biochar, and that the agronomic impacts of biochar compound with increasing biochar applications until reaching maximum benefit at 18 Mg ha−1. This amount of biochar would take eight years to be produced in pyrolytic cook-stoves and drum ovens using the rice residues produced on-site. The net present value (NPV) of producing rice in the two systems was calculated based on their expected streams of costs and benefits. Biochar addition enhanced the NPV of rice by 12% and reduced the non-renewable energy intensity by 27%, relative to System A, after eight years of application. The difference in NPV values between production systems significantly increased to 23% and 71% by crediting GHG emissions abatement in low and high carbon price scenarios, respectively. These findings demonstrate the potential economic benefits of converting rice residues to biochar for soil application.

Commodity chains, institutions, and domestic policies of organic and fair trade coffee in Bolivia

ABSTRACTFair trade and organic coffee are alternative production and trade systems designed to promote the equitable and environmentally sustainable production of coffee. The purpose of this study is to analyze the functioning of different coffee supply chains in Bolivia and find policy changes necessary to sustain specialty coffee production in that country. Based on the primary and secondary data collected from various market functionaries, we find that the Bolivian coffee growers and other intermediaries have benefited financially from participating in fair trade and organic markets. However, the producers continue to face challenges, including increasing costs of production, stagnant premium, price floors, and declining yield. Institutional reforms within the supply chain and greater support from the government are necessary to ensure a better functioning Bolivian market. The government should also prioritize certified coffee as a part of its national food sovereignty agenda since Bolivians consume increasing amounts of high-quality coffees.

Environmental impacts of irrigated and rain-fed barley production in Iran using life cycle assessment (LCA)

Current intensive grain crops production is often associated with environmental burdens. However, very few studies deal with the environmental performance of both current and alternative systems of barley production. This study was undertaken to evaluate energy consumption and environmental impacts of irrigated and rain-fed barley production. Additionally, three alternative scenarios were examined for irrigated barley fields including conservation tillage and biomass utilization policies. The findings showed that around 25 GJ/ha energy is needed in order to produce 2300 kg/ha irrigated barley and 13 GJ/ha for 1100 kg/ha rain-fed barley. Life cycle assessment (LCA) results indicated that irrigated farms had more environmental impacts than rain-fed farms. Electricity generation and consumption had the highest effect on the abiotic depletion potential, human toxicity potential, freshwater and marine aquatic ecotoxicity potential. However, alternative scenarios revealed that using soil conservation tillage systems and biomass consumption vs. gas for electricity generation at power plants can significantly mitigate environmental impacts of irrigated barley production similar to the rain-fed conditions while higher yield is obtained.

A Diagnosis of Biophysical and Socio-Economic Factors Influencing Farmers' Choice to Adopt Organic or Conventional Farming Systems for Cotton Production.

Organic agriculture is one of the most widely known alternative production systems advocated for its benefits to soil, environment, health and economic well-being of farming communities. Rapid increase in the market demand for organic products presents a remarkable opportunity for expansion of organic agriculture. A thorough understanding of the context specific motivations of farmers for adoption of organic farming systems is important so that appropriate policy measures are put in place. With an aim of understanding the social and biophysical motivations of organic and conventional cotton farmers for following their respective farming practices, a detailed farm survey was conducted in Nimar valley of Madhya Pradesh state in central India. The study area was chosen for being an important region for cotton production, where established organic and conventional farms operate under comparable circumstances. We found considerable variation among organic and conventional farmers for their social and biophysical motivations. Organic farmers were motivated by the sustainability of cotton production and growing safer food without pesticides, whereas conventional farmers were sensitive about their reputation in community. Organic farmers with larger holdings were more concerned about closed nutrient cycles and reducing their dependence on external inputs, whereas medium and small holding organic farmers were clearly motivated by the premium price of organic cotton. Higher productivity was the only important motivation for conventional farmers with larger land holdings. We also found considerable yield gaps among different farms, both under conventional and organic management, that need to be addressed through extension and training. Our findings suggest that research and policy measures need to be directed toward strengthening of extension services, local capacity building, enhancing availability of suitable inputs and market access for organic farmers.

Strategies to optimize capsid protein expression and single-stranded DNA formation of adeno-associated virus in Saccharomyces cerevisiae.

Adeno-associated virus type 2 (AAV) is a nonpathogenic parvovirus that is a promising tool for gene therapy. We aimed to construct plasmids for optimal expression and assembly of capsid proteins and evaluate adenovirus (Ad) protein effect on AAV single-stranded DNA (ssDNA) formation in Saccharomyces cerevisiae. Yeast expression plasmids have been developed in which the transcription of AAV capsid proteins (VP1,2,3) is driven by the constitutive ADH1 promoter or galactose-inducible promoters. Optimal VP1,2,3 expression was obtained from GAL1/10 bidirectional promoter. Moreover, we demonstrated that AAP is expressed in yeast and virus-like particles (VLPs) assembled inside the cell. Finally, the expression of two Ad proteins, E4orf6 and E1b55k, had no effect on AAV ssDNA formation. This study confirms that yeast is able to form AAV VLPs; however, capsid assembly and ssDNA formation are less efficient in yeast than in human cells. Moreover, the expression of Ad proteins did not affect AAV ssDNA formation. New manufacturing strategies for AAV-based gene therapy vectors (rAAV) are needed to reduce costs and time of production. Our study explores the feasibility of yeast as alternative system for rAAV production.

Finding the Most Significant Elements for the Classification of Organic Orange Leaves: A Data Mining Approach

ABSTRACTBrazil is the world’s largest producer of oranges. The Brazilian conventional citrus crop requires repeated application of agrochemicals to achieve satisfactory levels of productivity. The organic citriculture is an alternative production system, which is environmentally friendly and offers a safe food to consumers. However, it is difficult to determine if a food or plant was cultivated in organic or conventional system by just common observation, which makes the customers of organic food market vulnerable against fraudulent entrepreneurs. In this study, we present a data mining approach for the study of Brazilian organic citrus leaves which can aid in the certification of authenticity of the citrus leaves. The elemental composition is determined by inductively coupled plasma-mass spectrometry (ICP-MS). We developed classification models based on support vector machines and artificial neural networks capable of predicting whether a citrus leaf is organic or conventional through analysis of the concentration levels of the 14 chemical elements (Al, Ba, Co, Cr, Cs, Cu, Fe, Mg, Mn, Ni, Rb, Si, Sr, and V) found in both types of leaves. Feature selection filter methods are used to determine the most relevant elements for the classification process. Our best model obtained was a support vector machine with approximately 88% prediction accuracy. The elements Mn, Mg, and Rb were evaluated as the most significant for the classification decision. This is the first paper which addresses the problem of classification of organic orange leaves based on chemical composition. The presented methodology is useful for attesting authenticity of organic citrus leaves and can be adapted for other organic food or substances.

Preparation of hydrolytic liquid from dried distiller's grains with solubles and fumaric acid fermentation by Rhizopus arrhizus RH 7-13

Fumaric acid production from lignocellulosic materials is an alternative chemicals production system. This work investigated the suitable conditions for hydrolysis of dried distiller's grains with solubles (DDGS). The hydrolytic liquid was subsequently used for the production of fumaric acid. After optimizing the hydrolysis conditions, the most suitable concentration of H2SO4 (2%), hydrolysis temperature (120 °C), hydrolysis time (100min) and solid/liquid ratio (1:10) were obtained. The yield of monosaccharides reached 258 mg/g DDGS and 15.88 g/L glucose, 7.53 g/L xylose and 2.35 g/L arabinose were obtained in unprocessed hydrolytic liquid. The furfural inhibitor in the hydrolytic liquid was also detected and the yield of it was reducing progressively in the pretreatment process. The ferment ability of the hydrolytic liquid from DDGS was tested through the process of fumaric acid production by Rhizopus arrhizus RH 7-13. The unprocessed hydrolytic liquid was not appropriate for the fermentation process. The yield of fumaric acid from the concentrated processed hydrolytic liquid reached 18.93 g/L, which was close to the yield of fermenting 80 g/L glucose. This result indicated that the commonly used carbon resource glucose could to some extent be replaced by processed hydrolytic liquid.