Alternative Solution Research Articles

The Growth Engine Concept and Its Potential for an Electrified Aviation Future

Abstract The emerging need for cost- and energy-efficient propulsion systems have shifted the research interest to radical concepts, with several challenges to overcome in order to enter into service. However, revitalizing old yet provably successful engine designs—a practice followed by engine manufacturers over the years—can provide low-risk alternative solutions to move immediate market demands. The paper reviews this approach, known as concept of growth engines or core commonality, and investigates how a geometrically fixed or geometrically similar engine core can be utilized across a family of engines. The development programs of the highly successful CFM56 and PT6 engine families are analyzed, proving the success of this concept over the years. The idea is based on the engineering paradigm of product families and is briefly presented from a theoretical perspective. Implementation methods and simulation tools to initiate, conceptualize, design, and evaluate an engine family program are reviewed. The potential application of engine core commonality into electrified propulsion systems is investigated. Design challenges and opportunities that electrification imposes to the growth engine concept are discussed. Finally, the concept of growth electrified propulsion systems is introduced, conveying future research directions to achieve a successful family of engines for electrified applications.

Enhancing Traffic Flow and Safety at LMU Nurtanio Road Crossings - Abdul Rahman Saleh Intersection in Bandung

Bandung is the largest metropolitan city in West Java Province and serves as its provincial capital. One of the locations where vehicle queues or traffic congestion occurs is at the level crossings between the highway and railway tracks, including the direct crossing (JPL) at Andir on LMU Nurtanio Street - Abdul Rahman Saleh Street. The purpose of this research is to assess the performance of the existing road section, simulate it using the PTV VISSIM program, and find alternative solutions to the identified problems. In this study, primary data was obtained through direct field measurements, including vehicle volume, queue length, and road geometry data. Additionally, secondary data on the number of trains passing through the crossing was obtained from PT Kereta Indonesian Fire. The PTV VISSIM program used in this analysis is a student-licensed program, which implies limitations and simplifications in the analysis. The performance calculation of the road section yielded a degree of saturation (Ds) value of 0.73 for LMU Nurtanio Street and 0.77 for Abdul Rahman Saleh Street. According to the road service levels set by the government, both roads fall below service level C. The PTV VISSIM modeling results indicated a queue length of 66.59 m for LMU Nurtanio Street and 105.18 m for Abdul Rahman Saleh Street. Bandung study uses PTV VISSIM to assess LMU Nurtanio - Abdul Rahman Saleh Streets, revealing congestion issues. Queue lengths highlight improvement needs.

Safety and efficacy of a new covered stent in hemodialysis vascular access outflow stenosis: A Brazilian multicenter retrospective study.

Vascular stenosis commonly leads to dysfunction in hemodialysis vascular access. Although percutaneous transluminal angioplasty is an established treatment, stent utilization has increased in the last decade as an alternative solution to extend the access function. This study evaluated the safety and initial results of a new impermeable covered stent for treating vascular access outflow stenosis. Investigators retrospectively analyzed 114 hemodialysis patients treated with polytetrafluorethylene-covered stents from September 2018 to September 2022 across four centers. Lesions treated were de novo or restenotic and located in the venous graft anastomosis, outflow segment, cephalic arch, and basilic swing point. Patients were followed by in-person physical examination at 1, 3, and 6 months, and Duplex ultrasound was performed to evaluate the vascular access circuit and in-stent restenosis. The primary efficacy endpoint was target lesion primary patency at 1, 3, and 6 months. Secondary endpoints included access circuit primary patency and secondary patency at 1, 3, and 6 months. The primary safety endpoint was freedom from local or systemic serious adverse events through 30 days post-procedure. Forty-four patients had thrombosed access at the initial presentation, and 41 patients presented with recurrent stenosis. The target lesion primary patency rates at 1, 3, and 6 months were 100%, 89.4%, and 74%, respectively. The access circuit primary patency rates were 100% at 1 month, 85% at 3 months, and 62.7% at 6 months. The secondary patency rates at 1, 3, and 6 months were 100%, 96.4%, and 94.6%, respectively. In the adjusted multivariate Cox regression analysis, only recurrent lesions and female gender were associated with reduced primary patency rates. No serious adverse event was observed through the first 30 days post-procedure. In this retrospective analysis, a new covered stent was shown to be safe and effective for treating peripheral outflow stenosis in vascular access.

An Investigation of BIM Advantages in Analysing Claims Procedures Related to the Extension of Time and Money in the KSA Construction Industry

The construction industry in the Kingdom of Saudi Arabia (KSA) is a significant sector in the Middle East, with annual expenditures exceeding USD 120 billion. It employs 15% of the workforce and consumes more than 14% of the country’s energy resources. Despite the significant growth in the Saudi construction sector, it faces various challenges due to the rapid launch of mega projects, such as the Line project engaged with the NEOM project, as well as other new projects as part of the Saudi Vision 2030. The challenges might be limited to a shortage of skilled labourers, rising costs, construction disputes, and material shortages. This study aims to investigate claims management procedures under traditional practice and compare them with a proposed BIM package as an alternative solution to mitigate construction disputes. The objective of the study focuses on reducing the time consumed when analysing claims against the level of accuracy of claims values. The proposed BIM model improves and streamlines the claims process through automation. This study presents prospective and retrospective methods in delay analysis under an accepted programme. A questionnaire survey was conducted, and out of a total of 123 practitioners, 79 replied. The findings in tables in this article reveal that there are demands and a growing awareness of BIM in the KSA construction industry. The results reveal that BIM can help to reduce potential disputes and can reduce overall project cost overruns.

Artificial Intelligence Language Model Performance for Rapid Intraoperative Queries in Plastic Surgery: ChatGPT and the Deep Inferior Epigastric Perforator Flap

Background: The integration of artificial intelligence in healthcare has led to the development of large language models that can address various medical queries, including intraoperatively. This study investigates the potential of ChatGPT in addressing intraoperative questions during the deep inferior epigastric perforator flap procedure. Methods: A series of six intraoperative questions specific to the DIEP flap procedure, derived from real-world clinical scenarios, were proposed to ChatGPT. A panel of four experienced board-certified plastic surgeons evaluated ChatGPT’s performance in providing accurate, relevant, and comprehensible responses. Results: The Likert scale demonstrated to be medically accurate, systematic in presentation, and logical when providing alternative solutions. The mean readability score of the Flesch Reading Ease Score was 28.7 (±0.8), the Flesch–Kincaid Grade Level was 12.4 (±0.5), and the Coleman–Liau Index was 14.5 (±0.5). Suitability-wise, the DISCERN score of ChatGPT was 48 (±2.5) indicating suitable and comprehensible language for experts. Conclusions: Generative AI tools such as ChatGPT can serve as a supplementary tool for surgeons to offer valuable insights and foster intraoperative problem-solving abilities. However, it lacks consideration of individual patient factors and surgical nuances. Nevertheless, further refinement of its training data and rigorous scrutiny under experts to ensure the accuracy and up-to-date nature of the information holds the potential for it to be utilized in the surgical field.

Comparison of 2-Aminopyridine and 4-Thiopseudisocytosine PNA Nucleobases for Hoogsteen Recognition of Guanosine in RNA.

Peptide nucleic acid (PNA) is emerging as a promising ligand for triple-helical recognition of folded biologically relevant RNA. Chemical modifications are actively being developed to achieve high affinity and sequence specificity under physiological conditions. In this study, we compared two modified PNA nucleobases, 2-aminopyridine (M) and 4-thiopseudisocytosine (L), as alternatives to protonated cytosine (unfavorable under physiological conditions), to form more stable triplets than C+·G-C. Both nucleobases formed M+·G-C and L·G-C triplets of similar stability; however, the L-modified PNAs showed somewhat reduced sequence specificity. In conclusion, M and L represent two alternative solutions to the problem of cytosine protonation in triple-helical recognition of RNA. In M, the pKa is increased to favor partial protonation, which improves solubility and cellular uptake of M-modified PNAs. In L, the sulfur substitution enhances favorable hydrophobic interactions, which may have advantages in avoiding off-target effects that may be caused by cationic modifications. However, our results showed that substituting Ms with Ls did not restore the sequence specificity of a PNA containing cationic groups.

A sequence-aware merger of genomic structural variations at population scale

Merging structural variations (SVs) at the population level presents a significant challenge, yet it is essential for conducting comprehensive genotypic analyses, especially in the era of pangenomics. Here, we introduce PanPop, a tool that utilizes an advanced sequence-aware SV merging algorithm to efficiently merge SVs of various types. We demonstrate that PanPop can merge and optimize the majority of multiallelic SVs into informative biallelic variants. We show its superior precision and lower rates of missing data compared to alternative software solutions. Our approach not only enables the filtering of SVs by leveraging multiple SV callers for enhanced accuracy but also facilitates the accurate merging of large-scale population SVs. These capabilities of PanPop will help to accelerate future SV-related studies.

“Extra-anatomical Pedicle Rerouting” An alternative technique to obtain new recipient vessels for microsurgical reconstruction in unfavorable clinical situations

BackgroundLack of adequate recipient vessels in certain anatomically unfavorable locations or in complex clinical situations still represents a limit for a successful microsurgical transfer. To address such complex cases, advanced microsurgical techniques should be applied. In this paper, the authors describe their experience with the Extra-anatomical Pedicle Rerouting (EPR) technique, an alternative approach that was used in selected cases throughout the body to obtain healthy recipient vessels for microsurgical reconstruction in unfavorable clinical situations where suitable recipient vessels were difficult to find. Patients and Methods15 patients with defects of variable etiology (oncological resection, trauma, previous surgeries) located in the trunk, upper and lower extremities received EPR free flap reconstruction at our Institution. Operative data, post-operative course and complications were recorded. Clinical and photographical follow-up were also documented. ResultsA total of 15 flaps (6 ALT, 6 LD/TDAP, 3 DIEP) were transferred adopting the EPR technique for oncological (11) and post-traumatic (4) defects. The rerouted vessels according to the different clinical scenarios were the thoraco-acromial, posterior circumflex humeral, thoracodorsal, deep inferior epigastric, lateral circumflex femoral, anterior tibial and medial sural pedicles. Mean length of the rerouted vascular conduits was 6.53cm. Mean operative time was 420minutes. No major complications were registered. Minor wound dehiscence was observed and managed conservatively in 3 patients. ConclusionsThe EPR technique proved to be expendable in a reliable and reproducible manner in different regions of the body as an alternative solution to obtain healthy recipient vessels in anatomically and surgically unfavorable clinical situations.

Extended cervical approach for retrosternal multinodular goiter.

Partial or total sternotomy is required for 10% of retrosternal goiter. This study reviewed our experience with an extended cervicotomic approach as an alternative surgical solution for retrosternal goiter. A retrospective study was performed on patients who underwent partial or total thyroidectomy for retrosternal goiter between 2014 and 2019 at a tertiary medical centre. Data on clinical, radiologic, and pathologic factors were analysed. Peri- and postoperative outcomes were compared between extended and standard cervical approaches to predict the need for an extended cervical approach. The cohort included 265 patients, of whom 245 (92.4%) were treated by standard thyroidectomy. In 17 (6.4%), the standard approach proved insufficient, and the horizontal incision was extended to a T-shape to improve access. The remaining 3 patients required a sternotomy. Use of the extended cervical approach was significantly associated with clinical features such as male gender, diabetes, high body mass index and postoperative hypocalcaemia. The extended cervicotomic approach is an alternative surgical solution for retrosternal goiter, with no increased risk of significant post-operative complications.

Deep-Learning-Based Multiband Signal Fusion for 3-D SAR Superresolution

Three-dimensional (3-D) synthetic aperture radar (SAR) is widely used in many security and industrial applications requiring high-resolution imaging of concealed or occluded objects. The ability to resolve intricate 3-D targets is essential to the performance of such applications and depends directly on system bandwidth. However, because high-bandwidth systems face several prohibitive hurdles, an alternative solution is to operate multiple radars at distinct frequency bands and fuse the multiband signals. Current multiband signal fusion methods assume a simple target model and a small number of point reflectors, which is invalid for realistic security screening and industrial imaging scenarios wherein the target model effectively consists of a large number of reflectors. To the best of our knowledge, this study presents the first use of deep learning for multiband signal fusion. The proposed network, called <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$kR$</tex-math></inline-formula> -Net, employs a hybrid, dual-domain complex-valued convolutional neural network (CV-CNN) to fuse multiband signals and impute the missing samples in the frequency gaps between subbands. By exploiting the relationships in both the wavenumber domain and wavenumber spectral domain, the proposed framework overcomes the drawbacks of existing multiband imaging techniques for realistic scenarios at a fraction of the computation time of existing multiband fusion algorithms. Our method achieves high-resolution imaging of intricate targets previously impossible using conventional techniques and enables finer resolution capacity for concealed weapon detection and occluded object classification using multiband signaling without requiring more advanced hardware. Furthermore, a fully integrated multiband imaging system is developed using commercially available millimeter-wave (mmWave) radars for efficient multiband imaging. Using two mmWave radars, each with a bandwidth of 4 GHz operating at 60 GHz and 77 GHz, the proposed <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"><tex-math notation="LaTeX">$kR$</tex-math></inline-formula> -Net is employed to achieve an effective bandwidth of 21 GHz by robustly estimating the full-band signal. Additionally, the generalizability of the proposed technique is demonstrated across multiband sensing scenarios in the mmWave and terahertz (THz) frequencies. Extensive numerical simulations and empirical experiments are conducted to illustrate the superiority of our approach over existing methods for a diverse set of realistic 3-D SAR imaging scenarios.

A proposal for an alternative structural modular solution

Modular construction has gained significant attention as an innovative building method in recent years due to its numerous advantages over traditional construction. However, achieving simple yet high-performance modular structures presents unique challenges for structural performance, fabrication, and overall construction. To address these challenges, this paper proposes an innovative approach called the MICADO method, which uses a steel frame-supported modular system and prefabricated panels for exterior walls. This study aims to evaluate the structural performance of the MICADO method under seismic loads and different parametric studies, including plan shape, number of stories, roof systems, and openings. A nonlinear pushover analysis was conducted, and the results indicate that the MICADO method produces acceptable performance, high ductility, and an adequate safety margin against failure under seismic loads. Additionally, a linear analysis was used to evaluate the element resistance and structural stability, and the results show that plan shapes significantly affect structural performance due to load distribution. Moreover, the results indicate that location and number of openings significantly affect structural behavior due to the use of columns with higher stiffness. The study concludes that the proposed structural solution method is suitable for buildings with a maximum of two stories, measuring 12 m in length and 7 m in width.

Laboratory scale Clean‐in‐Place studies on the effectiveness of caustic and electrolysed water on the removal of dairy soils

AbstractThe present study was conducted to evaluate the efficacy of electrochemically generated, ready‐to‐use dilute solution of sodium hydroxide (0.04%) (eNaOH) against dairy soils in a Clean‐in‐Place (CIP) process of a model ultra‐high temperature (UHT) system. The UHT system was fabricated using 306 stainless steel material and comprised of a collection tank, processing tank with an oil bath, chiller bath, centrifugal pump, and the control panel. Milk deposition was obtained by repeatedly re‐circulating milk through the system and thereafter cleaning it using different solutions. Different physico‐chemical parameters were investigated. The pH of the exiting solution remained within the range of 6–8.5 for eNaOH solution relative to 9–13 for 1% sodium hydroxide (NaOH) solution. The turbidity of the exit wash declined only slightly when using eNaOH as opposed to logarithmic decline when using 1% NaOH. Inductively Coupled Plasma analysis showed an increased affinity of Ca (45%–51%) and Mg (81%–96%) toward eNaOH solution relative to 1% NaOH. Overall, the findings of this study suggest eNaOH as a possible alternative to increased longevity of food processing equipment and reduced occurrence of scaling.Practical applicationsThe current study provides an alternative cleaning solution to the dairy industry with better cleaning efficiency at 1/25th concentration of the current cleaning process. Electrolytically generated sodium hydroxide solution (eNaOH) at 0.04% is a non‐toxic, non‐corrosive, environmentally friendly solution that has shown to give promising results at 60°C as compared to 1% NaOH at 70°C. Inductively Coupled Plasma analysis showed an increased affinity of Ca (45%–51%) and Mg (81%–96%) toward eNaOH solution relative to 1% NaOH. Overall, the findings of this study suggest a possible alternative to increased longevity of food processing equipment and reduced occurrence of scaling.

A Fluorescent Sex-Sorting Technique for Insects with the Demonstration in Drosophila melanogaster.

Recent advances in insect genetic engineering offer alternative genetic biocontrol solutions to control populations of pests and disease vectors. While success has been achieved, sex-sorting remains problematic for scaling many genetic biocontrol interventions. Here, we describe the development of a genetically stable sex-sorting technique for female and male selection with a proof of concept in Drosophila melanogaster termed SEPARATOR (Sexing Element Produced by Alternative RNA-splicing of A Transgenic Observable Reporter). This elegant approach utilizes dominantly expressed fluorescent proteins and differentially spliced introns to ensure sex-specific expression. The system has the potential for adaptability to various insect species and application for high-throughput insect sex-sorting.

Investigating market-based opportunities for the provision of nutritious and safe diets to prevent childhood stunting: a UKRI-GCRF action against stunting hub protocol paper

BackgroundInadequate access to affordable, safe, desirable and convenient nutrient-dense food is one of the underlying causes of child stunting. While targeted nutrition-sensitive interventions (eg, backyard ‘nutri-gardens’) may increase dietary diversity...

Pemanfaatan Kombinasi Limbah Fly Ash dan Bottom Ash Dalam Campuran Mortar Untuk Paving Block

Various alternative solutions to reduce waste continue to be sought and developed. This includes fly ash and bottom ash which are waste products from burning coal, one of which is produced by Steam Power Plants (PLTU). So the increasing need for electricity will also be followed by an increase in the amount of waste. This type of waste is utilized in various fields of science, such as agriculture, industry and quite a lot in the construction sector. As a field that continues to develop along with people's need to live and move, utilization in the construction sector has great potential as a solution to reduce this waste. The use of fly ash and bottom ash in construction material mixtures as a quality enhancer or simply to reduce the use of natural materials continues to be carried out. This research will focus on the utilization of waste at a large percentage on a construction product scale. Substitution in the composition of the mortar mixture which is the initial product shows the effect of improving quality at a relatively low percentage in the variations used in this research. However, the full substitution of this waste as a substitute for natural materials, namely fine aggregate, is still in a fairly good range, especially in the composition of mortar mixtures with relatively low quality targets or in composition ratios with more aggregate material than cement or binder.

Efficacy of Yisui granule on myelodysplastic syndromes in SKM-1 mouse xenograft model through suppressing Wnt/β-catenin signaling pathway.

To unmask the underlying mechanisms of Yisui granule (, YSG) for the treatment of Myelodysplastic syndromes (MDS). Our study used an SKM-1 mouse xenograft model of MDS to explore the anti-tumor potential of YSG and its safety, assess its effect on overall survival (OS), and evaluate whether its mechanism is associated with the demethylation of the secreted frizzled related protein 5 (sFRP5) gene and suppressing Wnt/β-catenin pathway. Bisulfite amplicon sequencing was applied to detect the level of methylation of the sFRP5 gene; western blotting, immunofluorescence staining, and real-time Polymerase Chain Reaction were performed to detect DNA methyltransferase 1 (DNMT1), sFRP5, and other Wnt/β-catenin pathway-related mRNA and protein expression. The results showed that high-dosage YSG exerted an anti-tumor effect similar to that of decitabine, improved OS, and reduced long-term adverse effects in the long term. Mechanically, YSG reduced the expression of DNMT1 methyltransferase, decreased the methylation, and increased the expression of the Wnt/β-catenin pathway antagonist-sFRP5. Furthermore, components of the Wnt/β-catenin pathway, including Wnt3a, β-catenin, c-Myc, and cyclinD1, were down-regulated in response to YSG, suggesting that YSG could treat MDS by demethylating the sFRP5 gene and suppressing the Wnt/β-catenin pathway. Our findings demonstrated that YSG could be used alone or in combination with decitabine to improve outcomes in the MDS animal model, providing an alternative solution for treating MDS.

Functionally responsive hydrogels with salt-alkali sensitivity effectively target soil amelioration

The long-standing crisis of soil salinization and alkalization poses a significant challenge to global agricultural development. High soil salinity-alkalinity, water dispersion, and nutrient loss present major hurdles to soil improvement. Novel environmentally friendly gels have demonstrated excellent water retention and slow-release capabilities in agricultural enhancement. However, their application for improving saline-alkali soil is both scarce and competitive. This study proposes a new strategy for regulating saline-alkali soil using gel-coated controlled-release soil modifiers (CWR-SRMs), where radical-polymerized gels are embedded on the surface of composite gel beads through spray coating. Characterization and performance analysis reveal that the three-dimensional spatial network structure rich in hydrophilic groups exhibits good thermal stability (first-stage weight loss temperature of 257.7 °C in thermogravimetric analysis) and encapsulation efficiency for fulvic acid‑potassium (FA-K), which can enhance soil quality in saline-alkali environments. The molecular chain relaxation under saline-alkali conditions promotes a synergistic effect of swelling and slow release, endowing it with qualifications as a water reservoir, Ca2+ source unit, and slow-release body. The results of a 6 weeks incubation experiment on 0–20 cm saline-alkaline soil with different application gradients showed that the gradient content had a significant effect on the soil improvement effect. Specifically, the T2 (the dosage accounted for 1 % of soil mass) treatment significantly increases water retention (30 % ~ 90 %), and nutrient levels (30 % ~ 50 %), while significantly decreasing soil sodium colloid content (30 % ~ 60 %) and soil pH (10 % ~ 15 %). Furthermore, PCA analysis indicates that the addition of 1 % CWR-SRMs as amendments can significantly adjust the negative aspects of soil salinity and alkalinity. This highlights the excellent applicability of CWR-SRMs in improving saline-alkali agricultural ecosystems, demonstrating the potential value of novel environmentally friendly gels as an alternative solution for soil challenges persistently affected by adverse salinity and alkalinity.

Validity of instrument to measure mathematics teachers’ perceptions towards problem-based learning activities

&lt;span lang="EN-US"&gt;Problem-based learning (PBL) is a proposed method for teaching mathematics in primary education in the education 4.0 era. This method enables teachers to conduct effective instruction by allowing students to explore alternative solutions to problems. This study was conducted to develop and validate an instrument for evaluating primary school mathematics teachers’ perceptions of PBL activities. This research is quantitative, and a questionnaire was employed to collect data. The data obtained were analyzed descriptively utilizing Cronbach’s alpha and exploratory factor analysis (EFA). It is determined that Cronbach’s alpha is 0.885, with a factor eigenvalue greater than 1. The Kaiser–Meyer–Olkin (KMO) value for each construct is 0.50, whereas Bartlett’s test value is statistically significant (&amp;lt;0.5). Each item has a factor loading of 0.50 or higher and a standard deviation of ≥60%. This study’s results indicated that this instrument can be used to investigate primary school mathematics teachers’ perceptions of PBL activities involving group division, generating ideas and learning issues, identifying problems, self-directed learning, synthesis and application, reflection, and assessment.&lt;/span&gt;

Molecular dynamics simulations of PFAS—investigating alternative solutions in oleophobicity and hydrophobicity

Molecular dynamics simulations of PFAS—investigating alternative solutions in oleophobicity and hydrophobicity

A synthetic biology approach to assemble and reboot clinically relevant Pseudomonas aeruginosa tailed phages.

The rise in the frequency of antibiotic resistance has made bacterial infections, specifically Pseudomonas aeruginosa, a cause for greater concern. Phage therapy is a promising solution that uses naturally isolated phages to treat bacterial infections. Ecological limitations, which stipulate a discrete host range and the inevitable evolution of resistance, may be overcome through a better understanding of phage biology and the utilization of engineered phages. In this study, we developed a synthetic biology approach to construct tailed phages that naturally target clinically relevant strains of Pseudomonas aeruginosa. As proof of concept, we successfully cloned and assembled the JG024 and DMS3 phage genomes in yeast using transformation-associated recombination cloning and rebooted these two phage genomes in two different strains of P. aeruginosa. We identified factors that affected phage reboot efficiency like the phage species or the presence of antiviral defense systems in the bacterial strain. We have successfully extended this method to two other phage species and observed that the method enables the reboot of phages that are naturally unable to infect the strain used for reboot. This research represents a critical step toward the construction of clinically relevant, engineered P. aeruginosa phages.IMPORTANCEPseudomonas aeruginosa is a bacterium responsible for severe infections and a common major complication in cystic fibrosis. The use of antibiotics to treat bacterial infections has become increasingly difficult as antibiotic resistance has become more prevalent. Phage therapy is an alternative solution that is already being used in some European countries, but its use is limited by the narrow host range due to the phage receptor specificity, the presence of antiviral defense systems in the bacterial strain, and the possible emergence of phage resistance. In this study, we demonstrate the use of a synthetic biology approach to construct and reboot clinically relevant P. aeruginosa tailed phages. This method enables a significant expansion of possibilities through the construction of engineered phages for therapy applications.