Alternative Food Research Articles

Partitioning of proteins and small molecular weight compounds from alfalfa juice during ultrafiltration

Ribulose-1,5-biphosphate-carboxylase/oxygenase (RuBisCO) from alfalfa is a promising source of alternative food protein, but it is challenging to purify. Ultrafiltration shows potential for concentrating and fractionating RuBisCO from low molecular weight compounds present in the extracted juice, but there is limited information on their partitioning depending on the membrane pore size. Therefore, the objective of this study was to evaluate the partitioning of various compounds from the juice depending on membrane pore size. It was hypothesized that both membrane pore sizes will retain RuBisCO but might partition low molecular weight compounds within the matrix differently. RuBisCO was fully retained by both the 300 and 100 kDa polyethersulfone (PES) membranes, while ash (29.7%–35.4% dry matter (DM)), non-protein nitrogen (2.38%–2.53% DM), and free N-terminals (10.13–10.34 mg glutamic equivalent/100 mg DM) were transmitted through both membranes. The retentates showed a distinct yellow color when filtering with 100 kDa membranes, but not when using 300 kDa membranes. Although no significant differences in total polyphenol permeation were observed through a spectrophotometric assay, a metabolomics screening analysis showed that the 100 kDa membranes had less transmission of the flavonoids apigenin, chrysoeriol, tricin, and tricin glycosides, which might contribute to the yellow color. These results reiterate the importance of measuring individual polyphenols using hyphenated techniques as opposed to spectrophotometric total reducing capacity. Sixteen saponins were identified and their permeation was lower in the 100 than the 300 kDa membranes, indicating a better potential for higher degree of protein refinement for the latter. This study demonstrates for the first time the difference in partitioning of the small molecular weight components, using PES membranes of similar chemistry but different size, during ultrafiltration of alfalfa juice.

Milletomics: a metabolomics centered integrated omics approach toward genetic progression.

Producing alternative staple foods like millet will be essential to feeding ten billion people by 2050. The increased demand for millet is driving researchers to improve its genetic variation. Millets include protein, dietary fiber, phenolic substances, and flavonoid components. Its climate resilience makes millet an appealing crop for agronomic sustainability. Integrative omics technologies could potentially identify and develop millets with desirable phenotypes that may have high agronomic value. Millets' salinity and drought tolerance have been enhanced using transcriptomics. In foxtail, finger, and pearl millet, proteomics has discovered salt-tolerant protein, phytohormone-focused protein, and drought tolerance. Metabolomics studies have revealed that certain metabolic pathways including those involving lignin, flavonoids, phenylpropanoid, and lysophospholipids are critical for many processes, including seed germination, photosynthesis, energy metabolism, and the synthesis of bioactive chemicals necessary for drought tolerance. Metabolomics integration with other omics revealed metabolome engineering and trait-specific metabolite creation. Integrated metabolomics and ionomics are still in the development stage, but they could potentially assist in comprehending the pathway of ionomers to control nutrient levels and biofortify millet. Epigenomic analysis has shown alterations in DNA methylation patterns and chromatin structure in foxtail and pearl millets in response to abiotic stress. Whole-genome sequencing utilizing next-generation sequencing is the most proficient method for finding stress-induced phytoconstituent genes. New genome sequencing enables novel biotechnological interventions including genome-wide association, mutation-based research, and other omics approaches. Millets can breed more effectively by employing next-generation sequencing and genotyping by sequencing, which may mitigate climate change. Millet marker-assisted breeding has advanced with high-throughput markers and combined genotyping technologies.

Nutritional Composition, Physicochemical, and Sensory Properties of Snack Bars Produced from Catfish Head Powder (Clarias gariepinus) and Purple Sweet Potatoes (Ipomoea batatas var Ayumurasaki) as Emergency Food

Graphical Abstract Highlight Research 1. The development of snack bars from catfish head powder (CHP). 2. The CHP was rich in nutritional composition and could be an additive ingredient in snack bars. 3. Adding CHP increased protein, lipid, and fibre content in the snack bars. 4. Snack bars with the addition of CHP were acceptable on all sensory attributes. 5. Snack bars produced from CHP could be used as an alternative emergency food. Abstract During natural disasters, emergency food is often needed to meet the victims’ nutritional intake such as protein, fat, carbohydrates, and energy. As an alternative, catfish heads, which are usually rich in nutritional content, can be developed into a snack bar product as emergency food. This research aimed to evaluate the chemical, physical and sensory properties of snack bars produced from catfish head powder (CHP) and purple sweet potato flour (PSPF). This research consisted of several stages, namely making CHP and PSPF, preparing snack bars, and analysing snack bars' chemical, physical, and sensory properties. The ratios of CHP and PSPF in the preparation of snack bars stage were F0 (0:100), F1 (25:75), F2 (50:50), and F3 (75:25). The CHP contained 43.52% protein, 21.24% lipid, 4.6% fibre, and 384.38 Kcal/100 g total energy. The addition of CHP and PSPF affected the chemical (proximate and antioxidant activity), physical (redness and hardness values), and sensory (aroma and overall acceptance) properties of the produced snack bars. All snack bars had protein content that complied with Indonesian National Standards (INS) for cereal bar biscuits. The protein, lipid, carbohydrate, and total energy content of the snack bar were in accord with the standards of the United States Department of Agriculture (USDA), Food Standards Australia New Zealand (FSANZ), and emergency food standards. It is also revealed that F2 was the best treatment based on its chemical, physical, and sensory properties. Indeed, snack bars from CHP and PSPF can be utilised as an emergency food alternative.

Spatial proximity in ‘local’ Alternative Food Networks: a case study of AMAP in France

This paper explores the emerging paradigm of alternative food networks (AFN), with a focus on AMAP (Association pour le Maintien de l’Agriculture Paysanne) in France as a case study. Delving into the multifaceted concept of spatial proximity within such networks, this paper explores three hypotheses drawn from literature on the spatiality of AFN, analyzing both ‘local’ distances variability and the characteristics of agricultural and urban contexts where such local-based AFN take place. Methodologically, the study leverages an original, manually compiled database at a national scale encompassing AMAP producers and distribution points. Specific analytical protocols are developed combining traditional geographical approaches with machine learning techniques. Key findings reveal the influence of both population density and agricultural land availability on the distances between producers and selling locations. Moreover, the study discerns that the nature of products and their processing levels significantly shapes 'local' distances. Additionally, the paper offers insights into distinctive features of the morphological landscape associated with AMAP producers. These findings may serve as a catalyst for future inquiries into the spatial dynamics and potential spatial configurations of alternative food networks.

Reduction of Asparagine and Reducing Sugar Content, and Utilization of Alternative Food Processing Strategies in Mitigating Acrylamide Formation—A Review

Reduction of Asparagine and Reducing Sugar Content, and Utilization of Alternative Food Processing Strategies in Mitigating Acrylamide Formation—A Review

Antimicrobial metabolites from Probiotics, Pleurotus ostreatus and their co-cultures against foodborne pathogens isolated from ready-to-eat foods

BackgroundThe incidence of foodborne pathogens in ready- to-eat (RTE) can be attributed to various foodborne diseases. Most of the isolated microorganisms from RTE foods are resistant to common antibiotics and thus, resulted to treatment failure when commercially available antibiotics are administered. However, the secondary metabolites secreted by microorganisms can serve as alternative therapy that are reliable and safe. Secondary metabolites obtained from mono- and co-culture microorganisms can inhibit the growth of antibiotic-resistant microorganisms. Bioactive compounds in the secreted metabolites can be identified and utilized as sources of new antibiotics. In this study, antimicrobial activity of secondary metabolites from Lactobacillus fermentum, Saccharomyces cerevisiae, Pleurotus ostreatus, and their co-cultures were tested against foodborne pathogens isolated from RTE foods using agar well diffusion. The bioactive compounds in the metabolites were identified using gas chromatography-mass spectrometry.ResultsFrom a total of 100 RTE foods examined, Salmonella enterica, Shigella dysenteriae, Escherichia coli, Klebsiella pneumoniae (subsp ozaenae), Pseudomonas fluorescens, Clostridium perfringes, Bacillus cereus, Listeria monocytogens, and Staphylococcus aureus, Penicillium chrysogenum, Aspergillus flavus, and Aspergillus niger were isolated and displayed multiple antibiotic resistance. The secondary metabolites secreted by co-culture of L. fermentum + P. ostreatus + S. cerevisiae, and co-culture of P. ostreatus + S. cerevisiae have the highest (P ≤ 0.05) zones of inhibition (23.70 mm) and (21.10 mm) against E. coli, respectively. Metabolites from mono-cultured L. fermentum, P. ostreatus, and S. cerevisiae showed zones of inhibition against indicator microorganisms with values ranging from 8.80 to 11.70 mm, 9.00 to 14.30 mm, and 9.30 to 13.00 mm, respectively. Some of the bioactive compounds found in the metabolites of co-cultured microorganisms were alpha-linolenic acid (25.71%), acetic acid 3-methylbutyl ester (13.83%), trans-squalene (12.39%), pentadecylic acid (11.68%), 3- phenyllactic acid (30.13%), linolelaidic acid methyl ester (15.63%), and 4-O-methylmannose (53.74%).ConclusionRTE foods contain multiple antibiotics resistance pathogens. The pronounced antimicrobial activity of the secondary metabolites against microorganisms from RTE foods could be attributed to the presence of bioactive compounds in the metabolites. These metabolites can be exploited as alternative food preservatives, biopharmaceuticals and can be used towards better health delivering systems.

The impact of meat alternative food imagery on word-of-mouth and repurchase intentions

The impact of meat alternative food imagery on word-of-mouth and repurchase intentions

Mediterranean Diet gastronomic experiences using olive oil and goat cheese

The present study aims to develop three food formulations, spreadable olive oil, goat cream cheese, and a cold dessert made of carob and goat cheese, using local elements of the Mediterranean diet (MD), namely, olive oil, fresh goat cheese, and carob derivatives in addition to hydrocolloids. The final products, with the best spreadability, texture and consistency, were obtained using: a) methylcellulose 6% (w/v) for the spreadable olive oil; b) Locust Bean Gum (LBG) (0.5% w/w) for goat cream cheese; and c) Goat cream cheese (43.4%), carob flour (8.7%), glucose syrup (26.1%), olive oil (14.6%), powdered goat milk (3.8%), chia seeds (0.5%), LBG (0.7%), and cinnamon for the cold dessert. Subsequently, foods were refrigerated until evaluated by a consumer panel of 101 untrained tasters aged between 15 and 49 years. All the attributes were assessed on a scale from 1 to 5. The products were well accepted by the tasters with no significant differences registered among the different age groups regarding the food attributes, except for the sweetness of the dessert. The goat cream cheese obtained the highest acceptability (4.09 ± 1.16–3.88 ± 0.86) and creaminess (4.22 ± 0.80–4.33 ± 0.65) among the youngest consumers (<30 years), while the acceptability of spreadable olive oil was evaluated with values of 3.24 ± 1.33–3.17 ± 1.14 by the same age group, indicating the need to add seasonings, as salt substitutes, to improve the attributes flavour and aroma. The results highlight the potential of the three formulations to expand the range of food products and processing strategies in the hospitality, catering, and food industry, as well as to provide alternative foods associated with the MD food pattern.

Understanding residents’ choice of urban farming systems in the Kumasi metropolis of Ghana: Land use policy implications

The heightened food insecurity in urban settings has rekindled the debate over the promotion of urban agriculture (UA) as a sustainable alternative food supply source across the globe. This study, therefore, examines the determinants of urban residents’ decisions to engage in UA and their choice of agriculture practices. Cross-sectional data was solicited from 430 urban dwellers through a multi-stage sampling technique. The data was analysed using descriptive as well as binary and multinomial logit regression models. The descriptive analysis shows that the cultivation of crops (34.7 %) predominates urban agricultural practices before crop-livestock integration (32.6 %) and livestock farming (32.6 %). The study identifies gender, age, religious affiliation, years of formal education, location of residence, household size, food insecurity status, availability of vacant land, extension access, and household income as significant determinants of urban households’ decision to participate in UA. The multinomial logit regression analysis revealed that urban farmers’ decision to practice only crop production is invariant between livestock-only production and integrated crop-livestock systems. When compared to the base category of the integrated crop-livestock system, the findings indicate that farmers’ educational background, food insecurity, engagement in salaried jobs, location, extension, and credit access are the main drivers of the decision to practice either crop-only or sole livestock production systems. The findings of this study have useful implications for the formulation of UA programs based on the different farming systems practiced in the study location.

Genetic characterization and distinctiveness of forage cactus accessions in Northern Minas Gerais, Brazil

Forage cactus is a vital alternative food source for livestock in arid and semiarid regions. This study aimed to estimate the genetic dissimilarity among forage cactus genotypes grown in the semiarid region of northern Minas Gerais, Brazil. The study evaluated forage cactus accessions through morphological characteristics over different periods and molecular marker data. Key morphological traits assessed included cladode length, width, thickness, and the number of cladodes per plant. The experiments were conducted at the Agricultural Research Company of Minas Gerais (EPAMIG Norte) using a completely randomized experimental design with five replications in a split-plot arrangement. The design consisted of 21 forage cactus accessions in the plots and 7 evaluation times in the subplots. Morphological dissimilarities were compared individually (Scott-Knott test; p&lt;0.05) and jointly (Euclidean distance). Molecular genotyping involved DNA extraction from young cladodes using inter-simple sequence repeat (ISSR) oligonucleotides. Dissimilarity based on molecular markers was estimated using the Jaccard index. Separate dendrograms were generated for each analysis (morphological and molecular) using the unweighted pair group method with arithmetic mean (UPGMA), and a tanglegram was created to illustrate the relationships between the two dendrograms. Significant differences were found in cladode length and width and in the fresh and dry weight of plants. The molecular analysis differentiated genotypes at the species and genus levels, even among those that appeared morphologically similar. Comparing morphological and molecular data revealed both divergences and similarities, providing valuable insights into the diversity and structure within the groups.

Learning competence of the temperate facultative cleaner wrasse Thalassoma pavo (Labridae).

Cleaner fishes remove parasites from other fishes called "clients," thereby contributing to a healthier ecosystem. Although the behavior and learning abilities of dedicated and tropical cleaner fishes have been broadly studied, a limited number of studies investigated the behavior of facultative and temperate cleaner fishes and, to the best of our knowledge, none focused on their cognitive abilities. Here, we tested the learning abilities of a species of temperate facultative cleaner, the ornate wrasse Thalassoma pavo in laboratory conditions, based on two problems. These two problems, or tasks, are relevant in two different contexts: the first one, deemed as mutualistically relevant, the cue-based discrimination task, and the second one, the spatial-based discrimination task, which is relevant in a non-cleaning context, when fish navigate through their environment to find food and return to their territories. We found that T. pavo individuals were able to solve these two tasks but excelled at the spatial task rather than with the cue discrimination. The same individuals were also challenged to learn the reverse protocol of these tasks and were again most successful in learning the reverse spatial discrimination problem, but not the cue. Contrary to the dedicated cleaner wrasse Labroides dimidiatus, this temperate facultative cleaner wrasse is slower to learn mutualistic relevant problems but competent in solving spatially derived problems. This may be due to the specific demands of their socio-ecological environment, with facultative cleaners having a greater component of non-mutualistic skills (spatial component), which prepares them to search for alternative food sources if necessary (e.g., feeding on the substrate) or even to expand territories more easily and less prepared to deal with mutualistic exchanges compared to dedicated cleaners that specialize to become increasingly socially competent.

Buckwheat: An Underutilized Himalayan Crop with Multifaceted Nutraceutical Benefits.

The human population is growing and alternate food options are needed to provide food and nutritional security to mankind. Reduced agricultural output as a result of climate change and increased demand for grains because of continuous population growth have created a gap between demand and supply of food. Buckwheat is a pseudocereal crop plant with high nutritional value that can be included as an alternate food in our diet. It is a traditional crop plant grown in the high mountains of the Himalayas for food as well as fodder. It completes its life cycle in 3-4 months, so is mostly grown as a second crop in between main crops like maize and barley. It also acts as a green manure by improving the phosphorus content of the soil. Buck-wheat has high nutritional value as it is rich in essential amino acids, vitamin B, trace elements, and other nutrients. The main bioactive compounds identified in buckwheat are rutin, quercetin, isoquercetin, d-chiroinositol, resveratol, and vitexin, which are responsible for its pharmacolog-ical properties. Research focused on value addition by exploring its nutritional, pharmaceutical, and other alternative uses of commercial importance, is needed for reviving buckwheat cultiva-tion practices and its conservation. Considering the multifarious applications of buckwheat, this review summarizes the currently available knowledge on the agronomic and nutraceutical sig-nificance of buckwheat to project its value as a future crop in the avenue of agriculture and functional food.

Soy proteins modified using cavitation jet technology

Soy proteins are seen as a promising alternative food source for meat with environmentally friendly properties. The problem is that the functional properties of soy proteins do not meet the needs of the food industry, and some existing modification technologies have adverse effects. Recently, cavitation jet technology (CJT) has been studied because it generates high heat, high pressure, strong shear and strong shock waves. This review summarizes the history and mechanism of cavitation jets. The energy generated during the cavitation jet process can open molecular structures, and the shock waves and microjets generated can pulverize the materials by erosion. The impact of the CJT on the morphology, structure, and functionality of soy proteins is discussed. The impact of combining CJT with other techniques on the production of soy proteins was also reviewed. The modification of proteins using two or more methods with complementary strengths, avoiding the disadvantages of certain techniques, makes the modification of proteins more effective. One of the most prominent effects is the combined treatment of cavitation jets with physical techniques. Finally, the review provides a comprehensive analysis of the application of modified soy proteins in the food industry and highlights promising avenues for future research.

Investigating the role of women producers in alternative food networks implementing organic farming in Berlin Brandenburg

Investigating the role of women producers in alternative food networks implementing organic farming in Berlin Brandenburg

Effect of sonoprocessing on the quality of plant-based analog foods: Compatibility to sustainable development goals, drawbacks and limitations

Sonoprocessing (US), as one of the most well-known and widely used green processing techniques, has tremendous benefits to be used in the food industry. The urgent call for global sustainable food production encourages the usage of such techniques more often and effectively. Using ultrasound as a hurdle technology synergistically with other green methods is crucial to improving the efficiency of the protein shift as well as the number of plant-based analog foods (PBAFs) against conventional products. It was revealed that the US has a significant impact when used as an assistant tool with other green technologies rather than being used alone. It increases the protein extraction efficiencies from plant biomasses, improves the techno-functional properties of food compounds, and makes them more applicable for industrial-scale alternative food production in the circular economy. The US aligns well with the objectives outlined in the UN’s Sustainable Development Goals (SDGs), and Planetary Boundaries (PBs) framework, demonstrating promising outcomes in life cycle assessment. However, several challenges such as uncontrolled complex matrix effect, free radical formation, uncontrolled microbial growth/germination or off-flavor formation, removal of aromatic compounds, and Maillard reaction, are revealed in an increased number of studies, all of which need to be considered. In addition to a variety of advantages, this review also discusses the drawbacks and limitations of US focusing on PBAF production.

Food Taboo for Pregnant and Lactating Mothers: A Study among Two Indigenous Peoples in South Sulawesi

Indigenous people believe and practice food taboos as part of the traditional culture. Food taboo is associated with cultural beliefs and passed on to every generation, in particular for pregnant women The food taboos in the Ammatoa Kajang and Kaluppini indigenous communities are specific for pregnant and lactating mothers. This study aimed to explore the traditional knowledge and food taboo among indigenous peoples in South Sulawesi concerning maternal health. The data collection was used in-depth interviews (IDIs) and focus group discussions (FGDs) guideline. We asked for the explanation behind the food taboos practice that still exists in these two communities. We found that these two communities believed and practice food taboo that could hinder them from various nutritious food that is important during pregnancy and lactating period. We recommend the alternative food utilization of nutritious, safe, do not against the traditional beliefs, available, and affordable local food sources for pregnant mothers.

Agrifood Sustainability Transitions in Firms and Industry: A Bibliographic Analysis of Research Themes

There is a growing consensus that the modern food system lies at the centre of the great challenges facing humanity and that urgent and profound changes are required in the way that food is produced, processed, distributed and consumed. This review analyses sustainability transitions within agrifood systems, focusing on the role of firms and industries as defined by the Sustainable Transitions Research Network (STRN). This paper conducts the first systematic literature review using bibliometric analysis to assess the current state of research on this theme. The findings reveal a significant increase in publications related to firms and industries associated with agrifood sustainability transitions. Furthermore, the focus of current research is geographically concentrated in the European Union. The review identifies four key themes in the literature, based on the co-occurrence of keywords. These are agriculture, innovation, governance, food systems and agroecology. The review identifies an increasing awareness of the role of farmers in driving sustainability transitions at the farm level. Furthermore, there is an increasing awareness of the interrelated characteristics of the agrifood system, which acknowledges the need for sustainable innovations to occur at multiple stages of the agrifood system. The review also shows that there is growing evidence that innovations can occur through disruptive as well as incremental innovation and highlights the importance of governance influencing transitions. The existing literature raises questions about alternative food networks as sustainable innovations, their potential for significant change in the established food system and the validity of their claims regarding food equity and environmental sustainability. A key theme emerging from the literature is an ecological perspective that identifies the complex biological processes and ecosystems that form an integral part of agricultural production. These findings provide a greater understanding of the current literature landscape of agrifood sustainable transitions relating to firms and industries and lay a foundation for future research.

Platform openness and value: the mediation effect of user interaction and psychological distance

PurposeLinking platform governance and value, the article explains how platforms manage openness, which gives users access and authority, to shape the user experience and orchestrate interactive value co-creation within their ecosystem.Design/methodology/approachWe adopted an abductive approach, drawing on an embedded multiple case study of platform-based local alternative food networks. The strong symbolic dimension embedded into that context allowed for a full exploration of value in its interactive nature.FindingsPlatform openness influences value creation directly, but also through two mechanisms: one behavioural, involving interactions between users and sides, and the other cognitive, involving users’ abstraction level. Value co-creation goes beyond the online space and occurs in complex sets of interactions between users. Digital platforms can thus generate intrinsic value co-creation that enhances their utility.Originality/valueWe have adopted a marketing channel theory reading of openness and mobilised a broader conception of value as well as cognitive science to explain how this key element of platform governance affects value creation.

Leaf and seed yield response of vegetable amaranths to nitrogen fertilizers applications and harvesting frequency at Jimma, South West, Ethiopia

Amaranthus (Amaranthus cruentus) is an alternative food crop that requires fewer agronomic practices relative to other food crops. However, the yield of Amaranthus varies due to factors such as season, soil fertility, variety, and management practices. There is limited information available on certain agronomic practices for Amaranthus, such as harvesting frequency and N fertilizer application rates. The objective of this study was to determine the effects of nitrogen levels and harvesting frequencies on the growth, leaf and seed yield, and yield components of the Amaranth Madiira-2 cultivar at Jimma, under irrigation. The study used a randomized complete block design with a factorial arrangement of five N levels (0, 34.5, 69, 103.5, and 138) and three harvesting frequencies (weekly, every 2 weeks, and every 3 weeks), replicated three times. Data on phenological growth, yield, and yield components were collected and analysed using SAS software (version 9.3). The results revealed that the combined effect of nitrogen and harvesting frequency significantly influenced (p < 0.05) the days to 50% flowering and days to 50% physiological maturity. Leaf area, plant height, above-ground fresh weight, above-ground dry weight per plant at harvest, leaf yield, and seed yield were highly significantly influenced (p < 0.01). However, the thousand seed weight when a two-week harvesting frequency was followed after transplanting and 103.5 kg N ha−1 was applied. The leaf yield was found to be significantly influenced by the harvesting frequencies, with the highest yield of 30.33 tons ha−1 observed under this particular combination of factors. Similarly, the seed yield was also maximized at 3.23 tons ha−1 under the same conditions, with 103.5 kg N ha−1 and a three-week harvesting frequency. Therefore, these findings suggest that farmers can benefit from applying 103.5 kg N ha−1 with a two-week harvesting frequency for better leaf yield and 103.5 kg N ha−1 with a three-week harvesting frequency for better seed yield. However, it is important to note that this study was conducted only during one season and in one location. Hence, conducting the experiment again in real-world settings, across various seasons and locations, would be beneficial. This would allow for drawing more reliable conclusions and making appropriate recommendations.

Canadians' experiences of alternative protein foods and their intentions to alter current dietary patterns

Despite established evidence about the environmental and health benefits of alternative protein foods (APF), considerable knowledge gaps and misconceptions remain toward APF. Drawing on a national survey (1800+ responses), the study explores Canadians' experiences of APF and intentions to alter current dietary patterns focusing on four APF – plant-based alternative proteins (PBAP) – plant-based (PB) milk, PB meat, lab-grown (LG) meat, and other alternative proteins (OAP) – and two animal-based foods (ANBF)– milk and meat. Data were collected based on a proportional stratified sampling method from all regions of Canada and analyzed using econometric models. While PB milk was the most consumed APF, followed by PB meat, LG meat was the least tried among participants. Perceived complexity of APF discouraged individuals from cutting back on ANBF, while increasing the consumption of APF. Perceived relative advantage was positively related to the adoption of APF. Perceived compatibility strongly influenced individuals’ adoption of PB meat and OAP. Likewise, perceived trialability significantly influenced the adoption of PB milk and PB meat. Despite the perception that APF may have high health related risks associated with processing, additives, calorie and sugar content, this did not prevent individuals from consuming PB milk and OAP. However, perceived risks did affect intentions to alter dietary patterns in the next 12 months. Additionally, meat attachment and sustainability orientation significantly predicted current consumption decisions of APF and intentions to alter dietary patterns. Although sustainability orientation motivates dietary changes, it did not always lead to extreme shifts. Finally, several demographic variables (age, gender, and education), dietary preferences (being omnivore), and residential area and region had influence on current consumption decisions and future intentions. In conclusion, by controlling several factors and through a comparative analysis of various protein sources, the study offers insights into the interplay of innovation-adoption characteristics, perceived risks, meat attachment and sustainability orientation in understanding dietary choices and provides some implications for industry stakeholders and policies promoting APF.