Diabetes mellitus is a serious condition with potentially devastating complications that affects all age groups worldwide with the percentage of affected people increasing at an alarming rate. Inhibition of alpha‐amylase and alpha‐glucosidase are the most important strategies in controlling the postprandial blood glucose and invariably for the treatment of disorders in carbohydrate uptake, such as diabetes and obesity. There had been several efforts geared towards sourcing anti‐diabetic therapeutics, the most studied being plants and have been reported to effectively do this but quite a number of them are toxic to humans. Thus we investigated alternative means of managing the ailment using ant lion larvae extract. In this study, alpha‐amylase and alpha‐glucosidase inhibitory potentials of the ant‐lion larvae extract were investigated. About 0.50 g of live ant‐lion larvae samples were hand‐picked from the soil within the school premises and homogenized in 25 ml of 100 mM phosphate buffer at pH 6.0. The protein concentration of the extract was determined while alpha‐glucosidase and alpha‐amylase inhibitory activities were determined by calculating the percentage inhibition and the inhibitory concentrations at 50 percent (IC50) of the crude extract. The values were compared to that of acarbose, a standard inhibitor and the mode of inhibition determined. The protein concentration of the extract was 5.256 ± 0.062 mg/ml with an IC50 value of 13.329 ±1.739 mg/ml for alpha‐glucosidase and 0.710±0.040 mg/ml for alpha‐amylase inhibitory activities while acarbose gave lower IC50 values of 0.620±0.090 mg/ml and 0.399±0.004 mg/ml for alpha‐glucosidase and alpha‐amylase respectively. The extract was noted to exhibit uncompetitive inhibition for both enzymes, mode of action attractive for drug design. It can therefore be concluded that the crude ant‐lion larvae extract could be more potent if purified and therapeutically exploited in the management of Diabetes mellitus or other related diseases.Support or Funding InformationSelf SupportedThis abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.