EVALUATION OF PROBIOTIC CANDIDATE IN COMBINATIONWITH TUBER EXTRACTS FROM CORALLOCARPUS EPIGAEUS (ARN.)CL. FORASSESSMENT OF IN VITRO ALPHA AMYLASE INHIBITION ACTIVITY

Bhumika R Gehloth,Kalpesh B Ishnava

doi:10.15414/jmbfs.2018-19.8.3.863-866

Abstract

Probiotics are living microbial adjunct which has a beneficial effect on host. Plant extracts such as those from Corralocarpus epigaeus often shows antimicrobial effect. Thus, there is always confusion about the using of herbal medicine or plant extractsinco-administration with probiotics supplement. The aim of the study was to show the synergistic action of probiotic bacteria in combination with plant extract from Coralocarpus epigaeus on alpha amylase inhibition activity. Alpha amylase inhibitors offer an effective strategy to lower the levels of post prandial hyperglycemia via control of starch breakdown. The probiotic bacterial culture in combination with different solvents for plant extraction viz. hexane, chloroform, ethyl acetate and methanol showing higher alpha amylase inhibitory activity compare to organic plant extract. The present study shows that the control having zone of diameter(3cm) and methanolic plant extract (2cm) and with Lactobacillus (0.9cm) which isconsideredgood % inhibition (70%) compare to all organic solvent. While, the methanolic plant extract (2cm) and Bifidobacterium (1cm) also showed moderate % inhibition (66.66%) compare to all organic solvent. The results indicate that combination of plant extracts with probiotic cultures showing highest percentage of alpha amylase inhibition. This study revealed that synergistic actionis probiotic and plant extracts can be used to control post prandial hyperglycemia.

Highlights

Probiotics are defined as live microorganisms which when administered in adequate amounts confer a health benefit on the host (Iannitti, 2010)
Studies have recently indicated the role of Probiotics in the modulation of gut microbiota in diabetes conferring a beneficial effect (Caniand Delzenne, 2009).A number of reports confirmed experimentally and clinically that oxidative stress is involved in the pathogenesis and progression of diabetes mellitus as well as the subsequent complications (Ceriello et al, 2004)
Alpha amylase and glucosidase inhibitors are the potential targets in the development of lead compounds for the treatment of diabetes, inhibitors of this enzyme control starch degradation and slow the process of carbohydrate digestion result in reduction in glucose absorption and lowering serum glucose levels (Sivaraj et al, 2012)

Summary

INTRODUCTION

Probiotics are defined as live microorganisms which when administered in adequate amounts confer a health benefit on the host (Iannitti, 2010). The control of glucose level is important aspect for type 2 diabetes, control of starch degradation by enzyme such as α amylase and glucosidase play a key role in diabetes These enzymes are major digestive enzyme and help in intestinal absorption (Sudha et al, 2011). Alpha amylase and glucosidase inhibitors are the potential targets in the development of lead compounds for the treatment of diabetes, inhibitors of this enzyme control starch degradation and slow the process of carbohydrate digestion result in reduction in glucose absorption and lowering serum glucose levels (Sivaraj et al, 2012). Probiotic sachets named Lactobacilplus were collected from medical store Shakti Medical Store from Anand, Gujarat, India This each sachet contains Lactobacillus acidophillus,Lactobacillus rhamnosus, Bifidobacterium longum, Bifidobacterium bifidum.

Methods

AND DISCUSSION

Findings

CONCLUSION