All-female Groups Research Articles

Transcriptome profile of sex-related gene expression and effects of cyp19a1 siRNA-silencing on sex differentiation and determination in yellow perch

In this study, we first sequenced the whole genome of the yellow perch (YP, Perca favencens) and screened transcripts of brain, liver, and gonadal tissues from fish of different sexes. By comparing known female sex-related genes in fish such as cyp19a1 and dmrt1 with YP genome, we obtained full-length sequences through genomic homology analysis. Then an experiment was conducted to evaluate the effects of cyp19a1-siRNA interference on gonadal development and sex differentiation and determination in YP. Transcriptome analysis revealed that the gonadal tissue of female YP exhibited predominantly and differentially up-regulated expression of genes than males, and cyp19a1 was predominantly expressed in the gonads while cyp19a2 showed predominant expression in the brain. KEGG pathway enrichment analysis illustrated the metabolic pathways of the top 25 enriched genes and confirmed CYP19 (P450) pathway in YP. For the cyp19a1-siRNA interference experiment, four intermittent in vivo injections of siRNA were administered to interfere with cyp19a1 gene expression in an all-female group of YP. The results collected at 180 dph revealed that the siRNA interference resulted in an incomplete masculinization and hermaphroditism of all-female monosex YP, displaying the presence of male spermatocytes along with female oocytes. In contrast, tissues from the blank control and negative control groups only contained oocytes. The appearance of the gonads in the experimental group appeared more irregular, lacking the smooth surface and typical histoarchitecture observed in the blank control and negative control groups. In addition, the cyp19a1-siRNA treatment group had significantly (P < 0.05) lower body weight than the control groups, indicating that the interference with cyp19a1 might have affected the expression of estrogen, leading to a trend similar to the growth of males; similarly, the gonad weight and GSIs of the cyp19a1 group were significantly (P < 0.05) lower than those in the control groups, suggesting that the cyp19a1-siRNA interference might have led to a trend of male type of gonadal development. Meanwhile, the treatment group had significantly (P < 0.05) higher HSI and VSI levels compared to control groups, indicating that stimulation of external siRNA might have resulted in more vigorous liver metabolism processes. Furthermore, the expression level of the dmrt1 in the treatment group was significantly (P < 0.05) upregulated compared to the control groups, suggesting the correlation roles between these two genes in the regulation of sex development. The findings provide valuable insights for future research into the underlying mechanisms involved and hold significant promise for sex control in aquaculture.

Unlikely Friends in Patriarchal Lands: An Ecofeminist Reading of Rajasthani Folktale “Sonal Bai”

"Sonal Bai" is a popular folktale about a girl told by women in Rajasthani-speaking areas of north India to initiate young girls into adulthood. This paper investigates the metaphorical representation of a girl’s coming of age through her relationship with a Sandalwood tree analysing, "Sonal Bai" as an ecofeminist text. The story renders voice to socially prohibited themes of menstruation and women’s sensual desire as embodied in silent friendship between a sandalwood tree and Sonal (girl with golden hair). The paper highlights how the outwardly simple tale of “Sonal Bai” is in fact an encoded lesson for teenage girls, inverted as a relationship between two highly treasured “belongings”/ “commodities”—sandalwood and a unmarried girl—in a patriarchal society economically dependent on agriculture. The sandalwood tree is highly valuable for farmers; different parts of the tree are used to produce furniture, oil, fragrance and food items. It is also a part of religious rituals where its essence is used as tilak on idols in Hindu traditions. Rajasthani Language is spoken prominently in the desert regions of India (Thar Desert) and the existence of such a tree in a desert is as precious and rare as a girl with golden hair. The paper draws reference from recurrent invocation of olfactory senses of the listeners captured by the images of the sandalwood tree in the story. It also explores use of growth metaphors using ‘rhizome’ like imagery of sandalwood branches reaching the sky which become an escape route for a teenage girl hiding from her family. The temporal and spatial indicators are infused together in the story of Sonal Bai leading to an unlikely friendship between two prized possessions that eventually breaks with the arrival of a patriarchal archetype: a handsome young prince. This study discusses the prevalent katha culture in India where women exercise freedom of voice through singing and narrating tales within all-female groups. This site of independent feminine interaction in a highly patriarchal society is investigated using Bakhtin’s “Chronotope” to highlight culturally encoded lessons in folktales meant for young girls in Indian society as a means of informal education.

A case study: lessons learned from online tutorial to improve practice readiness for family medicine residents in Palestine

BackgroundGeopolitical and socioeconomic challenges limit faculty development and clinical teaching in Palestine and many other developing countries. The first, and still only, Family Medicine (FM) residency program is a four-year program based out of An-Najah University in the West Bank. Training in primary care clinics occurs in the final two years and there are many challenges to adequate supervision in the clinical setting that were exacerbated during the pandemic. To improve the readiness for practice skills of 13 Palestinian FM residents a three-month tutorial program was organized in 2020. A nongovernmental organization (NGO) that has worked to support Family Medicine development in the region engaged experienced British and American General Practitioners trained as tutors to offer online tutorials. We examined the program as a case study to understand the factors that facilitated or impaired a positive virtual learning environment in a middle/low income country.MethodsThe tutors and residents were divided into groups and met virtually between June and September 2020. Evaluations and session reports collected during the program, the text of an online chat, and responses to an online survey two years later were collected. Using thematic analysis techniques, we evaluated the value for the residents at the time and two years later and identified factors that facilitated or impaired a positive virtual learning environment.ResultsThemes of knowledge, skills, attitudes, cultural disconnects, and tutorial logistics emerged. The group with the most stable tutor pairing, including one Arabic-speaker familiar with the context, was the most engaged. The all-female group formed a chat group to share real-time case questions during clinical practice and focused on skills (e.g. conducting a thorough medication review) and attitudes (e.g. open to sharing and discussing uncertainties). Other groups were less cohesive.ConclusionsTransnational tutorials that focused on clinical thinking and decision-making skills were most successful when the tutorial pair was stable, offered familiarity with the language and addressed cultural differences. Intrinsic factors such as lacking the motivation to participate and extrinsic factors such as unstable internet and rolling electric cuts, and clinical structures that made applying new skills challenging were more difficult to address but must be considered.

Sex ratio potentially influence the complexity of social calls in Himalayan leaf-nosed bat groups

Animals living in a complex social environment emit diverse acoustic signals and thus serve as excellent subjects for understanding the evolution of acoustic communication. Previous studies have focused on the complexity of social calls in some group-living animals, yet the determinants of vocal complexity at the intraspecific level remain unclear. Here, we aimed to assess the influence of group composition on social call complexity in Himalayan leaf-nosed bats (Hipposideros armiger) in the non-breeding season. The bats divided into three groups with the same number of individuals but with different sex ratio compositions. We monitored social vocalizations for the all-male group, the all-female group, the mixed group, and also quantified vocal complexity for each group based on multiple acoustic metrics, including vocal repertoire, call sequences, the diversity index, and information capacity. The results showed that there were significant differences in the composition of call sequences among the three bat groups. The number of vocalizations was the highest in the mixed group, while the social call complexity was the highest in the all-male group, followed by the all-female group, and was the lowest in the mixed group. The results suggest that sex ratio potentially influence the vocal repertoire in Himalayan leaf-nosed bats. Our findings might provide a cue for vocalization research to investigate sex ratio in social groups as a potential driver for vocal complexity.

Developmental Programming-Aging Interactions Have Sex-Specific and Developmental Stage of Exposure Outcomes on Life Course Circulating Corticosterone and Dehydroepiandrosterone (DHEA) Concentrations in Rats Exposed to Maternal Protein-Restricted Diets

The steroids corticosterone and dehydroepiandrosterone (DHEA) perform multiple life course functions. Rodent life-course circulating corticosterone and DHEA trajectories are unknown. We studied life course basal corticosterone and DHEA in offspring of rats fed protein-restricted (10% protein, R) or control (20% protein, C), pregnancy diet first letter, and/or lactation second letter, producing four offspring groups-CC, RR, CR, and RC. We hypothesize that 1. maternal diet programs are sexually dimorphic, offspring life course steroid concentrations, and 2. an aging-related steroid will fall. Both changes differ with the plastic developmental period offspring experienced R, fetal life or postnatally, pre-weaning. Corticosterone was measured by radioimmunoassay and DHEA by ELISA. Steroid trajectories were evaluated by quadratic analysis. Female corticosterone was higher than male in all groups. Male and female corticosterone were highest in RR, peaked at 450 days, and fell thereafter. DHEA declined with aging in all-male groups. DHEA: corticosterone fell in three male groups but increased in all-female groups with age. In conclusion, life course and sexually dimorphic steroid developmental programming-aging interactions may explain differences in steroid studies at different life stages and between colonies experiencing different early-life programming. These data support our hypotheses of sex and programming influences and aging-related fall in rat life course serum steroids. Life course studies should address developmental programming-aging interactions.

The impact of visualizing the group on children's persistence in and perceptions of STEM

Women are underrepresented in STEM fields across the world. We investigate a perceptual mechanism that may contribute to this gender disparity beginning in early childhood. We explore how visual information about the gender composition of a group of scientists impacts children's persistence on a STEM task and their evaluations of group members. One hundred sixty-six 4- to 6-year-old children viewed one of four groups of scientists: all-male, all-female, a lone female among all-males, or a lone male among all-females. Whereas children's persistence on a STEM task did not change across conditions, their trait judgments did. Children judged the all-male and all-female group scientists as “hardworking,” but judged the lone female scientist as “smart.” However, they were as likely to judge the lone male scientist as “smart” as to judge him “hardworking.” The role of group visualization as a learning mechanism impacting children's perceptions of scientists as early as the preschool years is discussed.

Lifting spirits and building community: the social, emotional and practical benefits of all-female group singing

A mixed-methods concurrent identical design was implemented to explore the following research question: what are the emotional, social, and practical impacts of group singing for women from a regeneration area? Members of an all-female community choir based in an area of disadvantage participated in a focus group and survey. The most significant quantitative finding was an increase in the overall perceived level of health, which seemed linked to qualitative themes of 'positive emotions', 'redefining and reclaiming identity', 'choir as an extended family' and 'community interaction and impact'. Three themes arose from qualitative data: social impact, health and well-being aspects, and practical issues. Integrating qualitative and quantitative findings identified an overarching concept that 'choir participation may increase emotional and social health and well-being'. Group singing was found beneficial, with a potential to serve as a resource in cultivating resilience in individuals living in areas of disadvantage.

Prince(ss) Charming of the Japanese Popular Theatre

Prince(ss) Charming of the Japanese Popular Theatre

Effects of academic achievement and group composition on the quality of student-generated questions and online procedural prompt usage patterns

This study aims to examine if and how academic achievement and gender group composition affect the quality of online SGQ and the use patterns of procedural prompts provided to support SGQ activities. Forty-one university sophomores enrolled in an English as a foreign language class participated in a four-week study. All questions generated were categorized based on the revised Bloom’s taxonomy for quality evaluation, and a content analysis along the set of integrated online procedural prompts was conducted to reveal usage patterns. Five key findings were obtained: First, the provision of the online procedural prompts served as an efficacious learning scaffold to help the participants at both high- and low-academic achievement levels generate the most questions at high-cognitive levels. Second, based on the results of the Fisher’s exact test, no significant relationships were found between academic achievement and the quality of SGQ. Third, the participants in the all-male and mixed-gender groups generated the majority of their questions at high-cognitive levels, whereas the all-female group generated an equal number of questions at both low- and high-cognitive levels. Fourth, no significant relationships between the gender group composition and the quality of SGQ were found according to the chi-square test of independence. Fifth, the results of the content analysis revealed that while some same usage patterns related to online procedural prompts were exhibited by students at both low- and high-academic achievement levels and with different gender group compositions, slightly different usage patterns were observed.

Collective Identities, Black Girlhood and 60s Vocal Groups

Drawing on interviews with Black women who sang in all-female vocal groups during the late 1950s and early 1960s, I examine the important role played by integrated public and private schools in the formation of the 1960s girl group phenomenon. From talent shows to choir practice, locker rooms to hallways, Black girls took up audible space in institutions of higher learning whenever they harmonized with friends or acquaintances. The collective identities Black girls created in their vocal groups allowed them to challenge racial and gender stereotypes in the civil rights era while also modeling sisterhood and friendship for subsequent generations of girls.

Shakespeare inThe Wilds: Experimenting withThe Tempest

AbstractThis article discusses the TV series The Wilds (Amazon Prime, 2020) as an adaptational experiment with Shakespeare’s The Tempest in several respects: as one of the latest complex TV shows to engage with the Shakespearean legacy, it experiments with the serialization of Shakespeare’s plot. Further, being an ‘unmarked’ adaptation that never directly refers to Shakespeare, it tests the limits as to what is ‘Shakespeare’ and what is ‘not Shakespeare’ or ‘no longer Shakespeare’ in the adaptational rhizome created by The Tempest and its reworkings, among which Lord of the Flies, Brave New World, and the TV series Lost are of particular importance for The Wilds. Changing Shakespeare’s character constellation to an all-female group of teenage castaways overseen by a female social scientist modelled on Prospero who seeks to overcome master narratives of fraternal power struggles, colonial exploitation, and patriarchal sexual oppression, The Wilds tests which changes the Shakespeare material can and perhaps needs to undergo to continue to have cultural meaning today. As part of this radical re-gendering, the series multiplies and collates Miranda, Ariel, and Caliban to interrogate contemporary girlhood and the future of intersectional feminism. The article concludes by assessing how the series appeals to differently knowing viewers, some of whom will feel invited to partake in the Tempest experiment, while others will lose Shakespeare’s tracks in The Wilds.

Contextual slanguage as linguistic performance among female youth in Nigeria

The article explores the use of contextual slang as linguistic performance by three all-female friendship groups in Calabar metropolis, Cross River State, south-eastern Nigeria. I argue that slang constitutes critical components of the discursive practices of young urban Nigerian women in maintaining friendship and deviating from stereotyped cultural and linguistic norms. Drawing insights from the analytical tools of African feminism and linguistic ideology, the article discusses recurrent themes in young women’s contextual slanguage and the motivations for the use of these creative linguistic and cultural resources in defining participants’ authentic social selves and in enacting their different modes of belonging. Qualitative ethnographic data for the study were sourced from participant observations, semi-structured interviews and informal conversations with 30 participants. The study concludes that young urban women utilise contextual slang as indexical tools in their everyday narratives to negotiate meaning in relation to the experience of their social lives, to acculturate to male linguistic norms and to affiliate with ideologies that represent gendered identity.

Behavioral Changes of Solitary Housed Female Pygmy Slow Lorises (Nycticebus pygmeaus) after Introduction into Group Enclosures.

Simple SummaryMost pygmy slow lorises confiscated in the illegal pet trade cannot be released back into the wild and end up in captivity for the rest of their lives. In the wild, their home ranges are large relative to their body size and they maintain a fairly solitary lifestyle compared to other primates. With the aim of improving their captive wellbeing, we tested to see whether female–female group pairings were in the animals’ best interest by looking at their behaviors before and after being moved to social housing in a larger enriched enclosure. With one nesting site per animal and ample space to avoid each other, all females opted to nest in pairs, not alone, and spent their time socially engaged in affiliative behaviors. We found that female pygmy slow lorises are more social than often assumed and that housing them with conspecifics is probably beneficial to their well-being in captivity.Pygmy slow lorises (Nycticebus pygmaeus) are threatened with extinction in the wild. Their nocturnal lifestyle and small size make them difficult to study in their natural habitat, but increasing evidence suggests that they are more social than previously thought. Our study was designed to assess the sociability of pygmy slow lorises by transferring six adult females from solo cages into environmentally enriched group home cages at the Japan Monkey Centre’s Slow Loris Conservation Centre. Two females were paired to create one group, while the other four were placed together in a second group. We compared their social interactions, activity budgets, and postural behaviors before and after social housing was initiated. We found that all-female slow loris groups had a high degree of sociality, preferred to stay close to each other, nested together every night, and spent less time in locomotion and more time grooming than when living alone. These results suggest that female pygmy slow lorises actively seek companions when available. The captive housing of all-female groups of lorises could lead to better husbandry practices and improved animal welfare by allowing them to have conspecific companions. We conclude that isosexual groups of pygmy slow lorises should be preferred over single housing when possible.

Review: Black Diamond Queens: African American Women and Rock and Roll, by Maureen Mahon

Review: <i>Black Diamond Queens: African American Women and Rock and Roll</i>, by Maureen Mahon

Serum estrogen and its soluble receptor levels in Egyptian patients with acute leukemia: case-control study

BackgroundAcute leukemias are malignant neoplastic diseases that arise from either lymphoid [ALL] or myeloid [AML] cell lines that are distinguished by the proliferation of BM non-functional immature cells and subsequently released into the bloodstream. ALL is prevalent malignancy in young, while AML in older. Diagnosis is usually routinely performed through peripheral blood count and smear then confirmed by BM aspirate. It is remarkable to notice that leukemia can be manifested at high, low, and even at normal leucocyte count. While treatment results have improved steadily over the last decades in younger and adults, limited changes have been in survival among subjects of age > 60 years. Aim of the work is to measure the serum estrogen [E2] and its soluble receptor [ER] levels in acute leukemia patients and extrapolate its possible clinical significance. This study included 40 [20 females and 20 males] healthy volunteers clinically free from any disease, 40 [20 females and 20 males] AML patients, and 40 [20 females and 20 males] ALL. To all subjects, serum E2 and its soluble ER level were investigated by ELISA.ResultsSerum E2 [pg/ml] level was lower in AML and ALL female and male patients groups than control group. Serum ER [ng/ml] level was lower in AML and ALL female and male patients groups than control group.ConclusionEstimation of serum E2 and its soluble ER level is of edifying diagnostic value. Determination of serum E2 and its soluble ER level in AML and ALL patients is of value in deciding treatment therapeutic target protocol.

Changes in dermatology practice characteristics in the United States from 2012 to 2017

BackgroundDermatology practice has recently seen multiple changes. A better understanding of trends pertaining to dermatology practice setups is necessary.ObjectiveTo analyze the recent changes in dermatology practice in terms of geography, practice size, and gender distribution as well as to analyze the availability of dermatologists based on zip codes’ income levels.MethodsThis was a cross-sectional study. We extracted data on the sex and billing addresses of dermatologists from Medicare provider utilization and payment data for 2012 and 2017. We used 2017 tax returns data to calculate the poverty level for each zip code.ResultsBetween 2012 and 2017, the number of solo practitioners decreased, while that of dermatologists working in large groups increased. The southern region experienced the largest changes. The male-to-female ratio decreased. Dermatology practices mainly comprised mixed genders, with a higher proportion of all-male groups versus that of all-female groups, but this difference decreased over time. In the northeastern and western regions, more than one third of dermatologists were located in the wealthiest zip codes.LimitationsThe Medicare data may not be exhaustively representative of the dermatology workforce, and the zip codes of 489 dermatologists’ billing addresses were missing in the tax return dataset.ConclusionsThese findings provide an understanding of the recent changes pertaining to dermatology practice setups and of the substantial health care disparities based on geographic distribution.

Sexually Dimorphic Growth Stimulation in a Strain of Growth Hormone Transgenic Coho Salmon (Oncorhynchus kisutch)

Growth hormone (GH) transgenic fish often exhibit remarkable transformations in growth rate and other phenotypes relative to wild-type. The 5750A transgenic coho salmon strain exhibits strong sexually dimorphic growth, with females possessing growth stimulation at a level typical of that seen for both sexes in other strains harbouring the same gene construct (e.g. M77), while males display a modest level of growth stimulation. GH mRNA levels were significantly higher in females than in males of the 5750A strain but equivalent in the M77 strain, indicating sex and transgene insertion locus altered transgene expression. We found that acute estradiol treatments did not influence GH expression in either strain (5750A and M77) or the transgene promoter (metallothionein-B), suggesting that estradiol level was not a significant factor influencing transgene activity. The feminization of XX and XY fish of the 5750A and M77 strains generated all-female groups and resulted in equalized growth of the two genetic sexes, suggesting that the presence of the Y chromosome was not directly capable of influencing the GH transgene–mediated growth in a physiological female conditions. These data suggest that the difference in growth rate seen between the sexes in the 5750A strain arises from non-estradiol-mediated sex influences on gene regulation at the transgene locus. This study shows how genetic factors and transgene insertion sites can influence transgene expression with significant consequent effects on phenotype.

Production of market-size European strain Atlantic salmon (Salmo salar) in land-based freshwater closed containment aquaculture systems

Interest in land-based farms using recirculating aquaculture systems (RAS) for market-size Atlantic salmon (Salmo salar) continues to grow, and several commercial facilities are already rearing fish. Performance data for commercially available mixed-sex, all-female, and triploid all-female Atlantic salmon reared to market-size in freshwater land-based facilities, however, are limited, particularly for European strain fish. Accordingly, eight groups of European-sourced Atlantic salmon (five groups of diploid mixed-sex, two groups of diploid all-female, and one group of triploid all-female fish) were reared from eyed egg to market-size in a semi-commercial scale land-based aquaculture systems over five separate production cycles to quantify performance metrics. Fish reached market-size (4−5 kg) in 24.7–26.3 months post-hatch. Fish were reared at a mean water temperature of 12.3–13.7 °C from first feeding to a mean size of 466–1265 g, then 13.3–15.1 °C during growout. On average, all-female groups grew faster than mixed-sex groups; however, environmental conditions and performance of individual cohorts varied. In a comingled production cycle, diploid all-female salmon grew faster than triploid counterparts. Early maturation rates ranged from 0 % to 67 %, with a mean maturation rate of 34 % for diploid mixed-sex fish and 67 % and 13 % for two diploid all-female groups, respectively. Triploid all-female Atlantic salmon did not mature. This research confirms biological and technological feasibility of growing Atlantic salmon to market-size in land-based systems but controlling early maturation of diploid salmon remained a challenge under the conditions utilized in these trials. This research provides important data inputs to optimize operational and financial projections for existing and potential land-based Atlantic salmon farms.

Future Girls of STEM Summer Camp Pilot: Teaching Girls about Engineering and Leadership Through Hands-On Activities and Mentorship

“Future Girls of STEM” is a university-sponsored, summer camp outreach program that was designed and implemented by an all-female faculty group to increase young girls’ interest in pursuing future careers in science, technology, engineering, and mathematics (STEM). This paper discusses the logistics and results of the pilot Future Girls of STEM camp, which was held in the summer of 2019 for seven to twelve-year-old girls. The four-day summer camp featured female engineers from local industry, who shared personal stories of their careers and helped campers work through engineering and leadership activities. Researchers assessed whether completing the activities increased participants’ understanding of, interest in, and self-efficacy in engineering topics, majors, and careers. Results showed an increased interest and self-reported understanding of engineering topics after participants completed the camp, as well as high self-efficacy throughout the camp experience. The Future Girls of STEM program, and its hands-on, interactive activities, can be transferred to other locations or universities with varying resources available.

Kuuk, Skrap, and the Resistance Vernacular

How are gendered identities enabled, contested, and performed through Nordic popular music? Building on relevant approaches in popular music analysis, this article offers an investigation into the function/s of language and musical style in enabling and engendering agency and subjectivity via two case studies in Norwegian popular music.&#x0D; Gender and language are crucial factors in this. In a global context of popular music, bands and artists who choose to sing in their local language may be seen to take up marginal positions compared to artists who choose to sing in English, as the choice of language would naturally limit their audience. I argue that this overlooks the efficacy of using one’s local language to express points of view that are relevant on a local level; what is more, it overlooks the possibility of subverting globalized trends and using these to one’s own ends.&#x0D; In this article, I offer close readings of Norwegian-language albums by two all-female groups: the hip-hop duo Kuuk (Live fra Blitz) and the electronica duo Skrap (Atlantis). Applying Russell A. Potter’s (1995) concept of the ‘resistance vernacular’ as it has been expanded and operationalized by Tony Mitchell (2004), I contend that the bands’ use of their local language opens their music to a broader set of possibilities when it comes to subverting gender and genre norms at the same time as it enhances the music’s political potential.&#x0D; Working in discernible genres enables both bands to create music that expresses a feminist stance; in the case of Kuuk, deconstructing and subverting expectations of gendered behaviour through parodying hip-hop misogyny, and in the case of Skrap, drawing on strategic naïvety to steer clear of gender stereotypes.