Alkali Extraction Method Research Articles

HPLC analysis, optimization of extraction and purification conditions, biological evaluation of total protoflavones from Macrothelypteris viridifrons

The present study aims to evaluate phytochemical and pharmacological potential of total protoflavones from Macrothelypteris viridifrons. In the phytochemical study, an HPLC analysis method was established, and the optimal extraction and purification conditions were analyzed. The extractive condition was optimized as follows: the backflow extraction with 20 folds of 70% ethanol at 80◦C for 1 h twice. Moreover, by combining the alkali-extraction and acid-precipitation method with the macroporous resin purification technology, the final purity rate of total protoflavones was no less than 54.85%. In the pharmacological study, the total protoflavones from M. viridifrons showed a significant tumor-inhibitory effect in the H22 hepatoma cells transplantation model with a higher inhibitory rate of 55.76% in high dosage (100mg/kg) treatment group compared with the positive control group (20 mg/kg cyclophosphamide). Taken of all, these results support that protoflavones are the material basis of M. viridifrons as an anticancer folk medicine.

Isolation of Silica-Lignin Composites from Rice Husk and Their Adsorption to Cr(VI)

<p>Rice husk is the most abundant agricultural waste in Indonesia. It can be used as a precursor for multifunctional materials such as silica-lignin composite for adsorbents. Silica-lignin can reduce heavy metal content in an industrial waste, such as the content of Cr(VI). This study aims to isolate and characterize silica-lignin composite, and determines the isotherm type of silica-lignin for hexavalent chromium adsorption. The isolation from rice husk used an alkali extraction method. Confirmation of typical functional groups in the silica-lignin composite was characterized by FTIR, while the morphology was characterized by SEM, respectively. The results reveal that the silica-lignin isolation was successfully performed. FTIR spectra indicate a typical wavenumber of the silica-lignin. The SEM image of the composite showed homogeneous morphology. The silica-lignin adsorption process on hexavalent chromium followed a Brunauer–Emmett–Teller (BET) isotherm type indicated by multilayer adsorption with a surface area of 948.8421 mg. g<sup>-1</sup><em>.</em></p>

Effect of pH and holding time on the characteristics of protein isolates from Chenopodium seeds and study of their amino acid profile and scoring

Alkali extraction and acid precipitation methods were adopted to isolate protein from quinoa and album seeds of variety Chenopodium. Different pH dispersions (3–11) of isolated proteins were prepared and effects of pH and holding time on protein characteristics were evaluated. The pH-10 of extraction medium was found suitable for protein extraction on the basis of yield, purity, solubility and colour having isoelectric pH of 4.5. Yield and purity of protein isolates (PI) of quinoa and album varied from 8.12 to 12.22%; 74.19 to 85.07% and 7.71 to 10.98%; 77.16 to 86.12%, respectively. Overall, pH and time had significant effect on functional properties of PI of both seeds. Quinoa PI had higher emulsifying activity, emulsion stability, water binding capacity and dispersibility, whereas, foaming capacity and stability were higher for album PI. Nutritional indices were 64.20 and 64.58 for quinoa and album PI, respectively, whereas, amino acid scoring (FAO, 2013) indicated, isoleucine, leucine and valine as the limiting amino acids.

Mango Seed Kernel Agronomical Bio-Waste for Ecofriendly Cotton Dyeing: Optimization of Dyeing Period and Temperature

Typical synthetic colorant and their application processes include various negative eco-toxicological effect and human health risk. Intend to depose the impediments of synthetic dyeing herein mango seed kernel agronomical bio-waste was employed for sustainable dyeing of cotton fabric. 100% cotton knitted single jersey commercially scoured-bleached fabric having areal density of 160 grams per square meter was used for this study. Natural dye was extracted by alkali extraction method from raw seed kernel of Mangifera indica. Optimum dyeing temperature and duration were quantified. Colorimetric appearance of the dyed samples for different dyeing condition were evaluate by using CIE L*a*b* color space in terms of color co-ordinates, color strength (K/S), brightness index (BI) and degree of color levelness values. Dye fiber bonding stability of the dyed fabric was evaluated in terms of colorfastness to wash, water, perspiration, rubbing and light. Overall the colorfastness values were recorded as 4-5 except light fastness. Excellent colorimetric appearance and serviceability indicates future green coloration of cotton fabric through mango seed kernel as well as decent agronomical bio-waste management.

Comparative Study of Xylan Extracted by Sodium and Potassium Hydroxides (NaOH and KOH) from Bagasse Pulp: Characterization and Morphological Properties

Xylan is the second most abundant polysaccharide and the predominant hemicellulose component of soda bagasse pulp. The present endeavor focuses on increasing the value addition to underutilized agro-industrial residue such as bagasse. For this purpose, xylan was isolated by two conventional alkali extraction methods i.e. NaOH and KOH. The recovery rate and sugar composition of different reaction times and alkali consumptions were monitored with advanced method such as High Performance Liquid Chromatography (HPLC). The Fourier Transform Infrared Spectroscopy (FTIR) and Wide Angle X-ray spectroscopy (WAXS) were respectively employed to characterize the functional groups and Crystallinity Index (CrI) changes during the extraction process. It was explored that highest xylan recovery rates were obtained with 6% of NaOH at 120 min and 6% KOH at 45 min. The xylan morphology via WAXS was found that its structure to be amorphous. HPLC results also showed KOH had higher effectiveness than NaOH in terms of extracted xylan purity. Highest XGRs (Xylose to Glucose Ratios) were also achieved by KOH processes. Hence, this study contributes to the adequate utilization of agricultural residues, with promising potential for applications in the production of certain novel materials and chemical conversion industries.

Extraction of Polysaccharide from Spirulina and Evaluation of Its Activities.

Background Polysaccharide of Spirulina platensis (PSP) is a kind of water-soluble polysaccharide extracted from Spirulina platensis. It has been proved to have antitumor, antioxidation, antiaging, and antivirus properties. And it has a promising prospect for wide application. Objective This study aims to identify an extraction process for high-purity polysaccharide in Spirulina (PSP) through a series of optimization methods and then evaluates its initial antiaging activities. Methods Four kinds of extraction methods—hot-water extraction, alkali extraction, ultrasonic-assisted extraction, and freeze-thaw extraction—were compared to find the optimal one, which was further optimized by response surface methodology. PSP was obtained after the crude PSP was deproteinized and depigmented. The antiaging effects of PSP were preliminarily evaluated through in vitro cell experiments. Results The alkali extraction method was determined as the optimal method, with the optimized extraction process consisting of a solid-liquid ratio of 1 : 50, a pH value of 10.25, a temperature of 89.24°C, and a time of 9.99 h. The final PSP contained 71.65% of polysaccharide and 8.54% of protein. At a concentration of 50 μg/mL, PSP exerted a significant promoting effect on the proliferation and traumatic fusion of human immortalized epidermal cells HaCaT. Conclusion An extraction method for high-purity PSP with a high extraction rate was established, and in vitro results suggest antioxidation and antiaging activities.

Properties of Natural Fibers Separated from Chromolaena odorata and Mikania micrantha

ABSTRACTThis paper reports the possibility of separating natural fibers from two invasive alien plants Chromolaena odorata and Mikania micrantha. These two are considered as high-risk species on the basis their invasiveness and being a menace to the natural biodiversity of Kerala, India. Water retting and alkali extraction methods were used for the separation of stem fibers. Fibers obtained showed similar properties to currently used common fibers. Based on morphometric measurements and derived values, such as slenderness ratio, flexibility ratio, and Runkel ratio, these fibers are expected to be suitable for industrial purposes.

Modification of the water-insoluble hemicelluloses via free radical copolymerization in diluted alkali aqueous medium

The synthesis of hemicelluloses-g-polyacrylamide (PAM) was carried out in diluted alkaline aqueous solution via free radical copolymerization initiated by redox initiator composed of potassium persulfate (KPS)/sodium thiosulfate (Na2S2O3). The less substituted hemicelluloses, l-arabino-4-O-methyl-d-glucurono-d-Xylan (wis-AGX), are the main components of the hemicelluloses isolated from corncobs by the ultrasound-assisted diluted alkali extraction method and exhibit poor water solubility, which largely limit their applications. The factors, such as the initiator dosage, the monomer concentration, and the reaction time, which influenced both the grafting percent and the grafting efficiency, were investigated. The chemical structure and thermal and macromolecular weight properties of the graft copolymers were characterized by Fourier transform infrared spectrometer (FT-IR), 1H nuclear magnetic resonance spectroscopy (NMR), thermogravimetric analysis (TGA), and gel permeation chromatography (GPC). The results indicated that the PAM moieties were successfully introduced into the side chains of the hemicelluloses, and the graft copolymers showed good solubility in water. Such modification is suggested to find a possible new way for broadening applications of water-insoluble hemicelluloses, and the resultant graft copolymers might have potential applications, such as gels, films, adsorbents, coatings, or drug delivery systems.

SYNTHESIS AND CHARACTERIZATION OF HYDROXYAPATITE-COLLAGEN-CHITOSAN (HA/Col/Chi) COMPOSITE BY USING EX-SITU WET PRECIPITATION METHOD

Hydroxyapatite (HA) was included mineral of apatite is a bioactive ceramic material and biocompatible, which has similarity with the nature of the bone chemical structure. The synthesis of hydroxyapatite (HA) by using wet precipitation method consists of calcination, hydration, HA synthesizes, and thermal treatment. The solution 0.3 M (NH4)2.HPO4 was dropped on the suspension of 0.5 M Ca(OH)2 from tutut (Bellamya javanica) shells. While collagen is extracted from the waste fish (Later calcarifer) scales by the following acid and alkali extraction methods. Synthesis of HA/Col/Chi composite by using ex-situ wet precipitation method that has been formed from 2 ml collagen of 2%, 2 ml chitosan of 2% mixed with 2 gram of HA powder was dissolved in 20 ml of an ethanol solution at room temperature. The spectrum of HA/Col/Chi composite by FTIR showed the presence of functional groups of HA, collagen, chitosan, and indicate the presence of molecular interactions between -OH, -COOH, -NH2 on collagen chains and -OH, -NH2 on chitosan, and there is an interaction between HA, amide I, and amide II in collagen. X-Ray Diffraction Analysis of HA/Col/Chi composite showed the single phase of HA. Analysis element components of HA showed that the Ca/P ratio obtained is 1.98. In the HA analysis by scanning electron microscopy showed the formation of HA in the presence of agglomeration and has an irregular shape. In addition, has a size of 2.76 - 6.48 µm. Keywords: Bellamya javanica, chitosan, collagen, ex-situ, hydroxyapatite, Lates calcarifer

Characterization and Antiproliferative Effect of Novel Acid Polysaccharides from the Spent Substrate of Shiitake Culinary-Medicinal Mushroom Lentinus edodes (Agaricomycetes) Cultivation.

In this study, a high yield of crude polysaccharide (16.73 ± 0.756%) was extracted from the spent mushroom substrate of Lentinus edodes using a hot alkali extraction method. Two groups of polysaccharides (designated as LSMS-1 and LSMS-2) were obtained from the crude extract by size exclusion chromatography (SEC), and their molecular characteristics were examined by a multiangle laser-light scattering (MALLS) and refractive index detector system. The weight-average molar masses of LSMS-1 and LSMS-2 were determined to be 6.842 × 106 and 2.154 × 106 g/mol, respectively. The SEC/MALLS analysis revealed that the molecular shapes of LSMS-1 and LSMS-2 were sphere-like forms in aqueous solution. Carbohydrate composition analysis using chromatography--mass spectrometry revealed that they were both acid heteropolysaccharides. LSMS-1 comprised mainly glucose and galacturonic acid, whereas LSMS-2 mainly consisted of xylose and glucuronic acid. Fourier transform infrared spectral analysis of the purified fractions revealed typical characteristic polysaccharide groups. In addition, MTT assays with refined polysaccharide doses of 25, 50, 100, 200, and 400 µg/mL suggested that both of the polysaccharide fractions exhibited antiproliferative activity against 6 tested human tumor cell lines in a concentration-dependent manner, and LSMS-2 had better anticancer capacity in vitro than LSMS-1. The inhibition ratio of LSMS-2 against A549 human lung cancer cells, the SGC7901 gastric cancer cell line, MCF-7 breast cancer cells, the U937 histiocytic lymphoma cell line, and the MG-63 human osteosarcoma cell line reached 43.55%, 29.97%, 19.63%, 18.24%, and 17.93%, respectively, at a concentration of 400 µg/mL.

A nanocomposite film fabricated with simultaneously extracted protein-polysaccharide from a marine alga and TiO2nanoparticles

This paper describes an edible nanocomposite packaging film fabricated with simultaneously extracted protein-polysaccharide (biopolymers) from the marine red alga Gracilaria lemaneiformis and TiO2 nanoparticles. Both proteins and polysaccharides were synchronously extracted using the alkali extraction method. Different amounts of TiO2 nanoparticles (TiO2 to biopolymer content ratio of 3.33, 5.00 and 10.0% w/w) were added to prepare the TiO2-protein/polysaccharide nanocomposite film. The addition of TiO2 nanoparticles increased the tensile strength and decreased the water vapor permeability of the film. This was because the uniformly embedded TiO2 nanoparticles interacted strongly with the biopolymers which enhanced the mechanical properties of the film, whereas the particles increased the diffusion pathway distance and lead to lower water permeability. These hypotheses were confirmed by UV spectra, FTIR, and X-ray diffraction studies. Antibacterial measurements against food-borne pathogenic bacteria revealed that the growth of Escherichia coli and Staphylococcus aureus was effectively inhibited by the TiO2-biopolymer nanocomposite film. Additionally, nutrition retention and quality improvement were observed in cherry tomatoes when coated with the TiO2-biopolymer nanocomposite film. These findings suggested that this alga-sourced nanocomposite film could serve as a promising coating material in the food industry.

Preparation and characterization of activated carbon & amorphous silica from rice husk

Activated carbon (AC) was prepared by the conventional carbonization and KOH activation and amorphous silica was extracted by alkali extraction followed by acid precipitation from rice husk on a laboratory scale. The performance of the produced activated carbon and amorphous silica were examined using I2 value measurement, methylene blue (MB) adsorption test, pH measurement, FTIR and SEM-EDX analysis. The optimum temperature for production of AC was obtained at 700 0C. The AC by alkali extraction method was show higher activity than KOH activation. The maximum I2 value, MB adsorption value & pH value shows at 700 0C followed by alkali extraction with 15% NaOH, which were 510.82 mg/g, 61.1 mg/g and 7.32 respectively. The FTIR analysis shows presence of Si-O-Si bond with a strong peak at 1078.28 cm-1. The SEM image of silica sample shows that the most of organic component is burnt out during combustion.Bangladesh J. Sci. Ind. Res. 50(4), 263-270, 2015

Separation of boron from borated paraffin wax by pyrohydrolysis and alkali extraction methods and its determination using ion chromatography.

A method based on the pyrohydrolysis extraction of boron and its quantification with ion chromatography was proposed for paraffin waxes borated with H3BO3 and B4C. The optimum pyrohydrolysis conditions were identified. Wax samples were mixed with U3O8, which prevents the sample from flare up, and also accelerates the extraction of boron. Pyrohydrolysis was carried out with moist O2 at 950°C for 60 and 90 min for wax with H3BO3 and wax with B4C, respectively. Two simple methods of separation based on alkali extraction and melting wax in alkali were also developed exclusively for wax with H3BO3. In all the separations, the recovery of B was above 98%. During IC separation, B was separated as boron-mannitol anion complex. Linear calibration was obtained it between 0.1 and 50 ppm of B, and LOD was calculated as 5 ppb (S/N = 3). The reproducibility was better than 5% (RSD).

Humification degree of peat and its implications for Holocene climate change in Hani peatland, Northeast China

The humification degree of peat is a significant climatic proxy for paleoclimate change. Using the alkali-extraction method, a time series of absorbance values of the Hani peatland, Northeast China, was determined, which is used as an indicator for the humification degree of peat. Combined with 14C dating data of peat cellulose, and compared with δ18O and δ13C time series of the cellulose in the Hani peatland, the evidence for the existence of 14 ka paleoclimate was provided. Higher humification degrees hint a warmer-wetter climate, and vice versa. It also reconstructs the four stages of Holocene climate evolution in this region: 11.5–9.8 cal ka B.P., warm and wet period; 9.8–9.0 cal ka B.P., cold and dry period; 9.0–4.8 cal ka B.P., warm and wet period; and 4.8–0 cal ka B.P., warm-wet and dry-cold alternation period. Meanwhile, it is revealed that the abrupt climate shifts signals such as the “8.2 ka” event and the “4.2 ka” event. Results showed that the Hani peat humification degree is of sensitive response to paleoclimate change. Therefore, it is a feasible method to analyze the relationship between paleoclimate change and peat humification degree.

Extraction of polysaccharides from Fomes officinalis Ames and their antitumor activity

The aim of this study was to optimize the extraction parameters of Fomes officinalis Ames polysaccharides (FOAPs) and evaluate their antitumor activity. FOAPs were extracted using the hot water extraction, acid extraction and alkali extraction methods, respectively. Alcohol precipitation and acetone washes were conducted to separate and purify the FOAPs. The FOAP content was determined using the phenol-sulfuric acid method. The effects of raw material particle size, extraction time and material-liquid ratio on the yield of FOAPs were investigated, and the effects of FOAPs on the immune function of S180 tumor-bearing mice and their antitumor activity were evaluated. The yield of FOAPs obtained with the hot water extraction method was higher compared with the yields of the other methods. The optimum extraction conditions were as follows: a raw material particle size of 24 mesh; an extraction time of 2.5 h; and a material-liquid ratio of 1 g:12 ml. Under these conditions, the yield of FOAPs was 1.13%. FOAPs significantly inhibited tumor growth and enhanced the immune function in S180 tumor-bearing mice. FOAPs extracted using the hot water extraction method have antitumor activity.

Extraction and separation of fucoidan from Laminaria japonica with chitosan as extractant.

Herein the extraction method of fucoidan from Laminaria japonica is reported. Firstly, chitosan, chitosan-N-2-hydroxypropyl trimethyl ammonium chloride (HACC), and hexadecyltrimethylammonium bromide (CPAB) were used to extract the fucoidan. The results showed that chitosan was the optimal extractant compared with the other two extractants. After extraction, different aqueous solutions, including NaCl, KCl, and HCl (pH2), were used to separate fucoidan from chitosan-fucoidan complex. The results showed that the separation ability of NaCl was slightly higher than that of KCl. Moreover, the price of NaCl is lower than that of KCl. Given the quality-price rate, NaCl solution was chosen as the separation solution. Thirdly, the concentration and ratio of NaCl solution : sediment influence the separation of fucoidan from chitosan-fucoidan complex. The results showed that the optimal separation conditions include 4 mol/L NaCl solution with the ratio of NaCl solution to sediment at 30 : 1. Fucoidan content was found to be affected by different separation time. Fucoidan content increased with the increase of separation time, and the optimal separation time was 6 h. Compared with traditional alkali extraction method, this method not only reduces the usage of alkali and acid and alleviate environment pollution, but also has the comparable extraction yield of fucoidan. It is a potential method for extraction of fucoidan.

Preparation of biodegradable polymer materials using agricultural waste

Polymer materials such as plastics, synthetic fibers and rubbers play an increasingly important role in our everyday lives. Most widely used polymeric materials developed in the past 50-60 years are durable and inert in the presence of microbes thus leading to a long term performance. However, in view of the current emphasis on environmental pollution problems and in conjunction with the land shortage problems for solid waste management and pending legislation, the need for biodegradable and „environmentally friendly‟ polymers came up. There are a number of applications (packaging, agriculture, household use, and more) where biodegradable polymers and composites are particularly suitable as sustainable alternatives. The aim of this study was to prepare a biodegradable polymer material using agricultural waste. Rice straw is one of the main cereal straws and is produced in large quantities in Sri Lanka. Rice straw was used as a main ingredient in this research to prepare the biodegradable polymer material. At present, these waste streams are not economically re-used and will create even larger problems for air and surface water pollution and having therefore a negative effect on people and planet.Rice straw which was an abundant biomass also consists of cellulose, hemicelluloses, lignin and others such as ash extractives and wax. Approximately 80% of yield of cellulose - hemicelluloses mixtures were extracted from rice straw using alkali extraction methods. Extracted products were identified and characterized by using Fourier Transform Infrared Spectroscopy (FTIR). Biodegradable polymer material was prepared by using Low Density Polyethylene (LDPE) and cellulose – hemicelluloses mixtures. Products were prepared by varying cellulose – hemicelluloses concentration. Biodegradable polymer mixtures were prepared by using laboratory scale internal mixture. Hydraulic press was used to prepare the LDPE - cellulose – hemicelluloses polymer products.Degree of biodegradability of the developed LDPE - cellulose – hemicelluloses product was measured by using soil burial test. Time dependent stress and strain properties were evaluated by tensile test. Water absorption test was also performed to evaluate the degradability. Weight loss gradually increased in all cellulose – hemicelluloses containing samples with increase of time in soil burial test. Water absorption test results also showed similar pattern. Tensile strength and percentage elongation of LDPE – cellulose –hemicelluloses product gradually reduced with the time in the soil burial test. This study suggests that rice straw has a good potential in obtaining cellulose and hemicelluloses to prepare biodegradable polymer materials for different applications. Key words: LDPE, biodegradable, cellulose, hemicelluloses

잎새버섯(Grifola frondosa) 균사체의 기능성 다당류 최적 추출방법 및 항암효과

Abstract Grifola frondosa has been used as an herbal medicine for the treatment of cancer, diabetes mellitus and high blood pressure. In this study, functional polysaccharide was obtained from Grifola frondosa using four different extraction methods: hot water(HwFP), homogenize(HgFP), acid(AcFP), and alkali(AlFP) extraction methods. The effects of these extracts on KB and HepG2 cell lines were then examined for any anti-cancer activity. Alkaline extraction produced a yield of 0.175% and the total sugar content of the extract was 54.97%. We were able to confirm that the polysaccharide extracts from the mushroom produce an anti-cancer effect. The cytotoxicity of AlFP and AcFP against HepG2 cells were 22.86% and 28.88%, respectively, and the cytotoxicity of AlFP against the KB cell lines was 47.76% at a concentration of 1,000 ㎍/㎖. Therefore, these results suggest that the optimum method for extracting functional polysaccharides from G. frondosa is the alkali extraction method.Key words: Grifola frondosa, polysaccharide, alkali extraction, anti-cancer effect

Physical Properties of Hemicellulose Films from Sugarcane Bagasse

Agricultural waste such as sugarcane bagasse can be obtained in large quantities from sugar and alcohol industries. Normally sugarcane bagasse has been used as a fuel to generate power of sugar mill. However, a huge quantity of the remaining bagasse is not used and burnt in the fields which can cause environmental problems. In recent years, many researchers show interest in utilization of agricultural waste to value-added products. Sugarcane bagasse contains about 33.5% hemicelluloses, which can be further utilized to produce other useful applications. The applications of material from hemicelluloses that have been identified include packaging films, food coatings, cationic biopolymers, hydrogels and biomedical uses. The objective of this study is to develop films from sugarcane bagasse hemicelluloses and to characterize the physical properties of hemicelluloses films. Biodegradable films were developed from hemicelluloses from sugarcane bagasse which was extracted using alkali extraction method. The alkali extraction of hemicelluloses was carried out at optimum condition with 3% NaOH concentration, temperature at 55 °C and pH 5.5. Four different volumes of ethanol and acetic acid solution were added to the supernatant to produce hemicelluloses. The hemicelluloses A, B, C and D were obtained from 85 ml of ethanol solution containing 10% acetic acid, 85 ml of cold ethanol solution, 4 volumes of ethanol, and 2 volumes of ethanol solution containing 0.2 volumes acetic acid, respectively. Films A, B, C and D were produced by casting the film-forming solutions, followed by solvent evaporation at a temperature of 40 °C and relative humidity of 52.9% in a controlled environment. The physical properties such as thickness, solubility, water vapor transfer rate, surface structure and color of the hemicelluloses films from sugarcane bagasse were investigated. All the extracts of hemicelluloses produced self-supporting films with thickness ranging from 0.13 mm - 0.15 mm, 36.9 - 67.1% solubility in water, 250.4 - 483.3 g/(m 2 .day) water vapor transfer rate (WVTR) and 0.31 - 1.72 MPa of tensile strength. The surface structure and color of the hemicelluloses films A, B, C and D are different probably due to the content of lignin in each of the film. This study suggests that hemicellulose of sugarcane bagasse has a good potential for production of biodegradable films for certain applications.

Extraction, characterization and utilization of rice bran protein concentrate for biscuit making

PurposeThe purpose of this paper is to study the utilization of the rice bran protein concentrate (RBPC) in biscuits to upgrade the nutritional quality.Design/methodology/approachExtraction of rice bran protein concentrate (RBPC) from defatted rice bran was standardized using alkali extraction method. Defatted rice bran and RBPC were analyzed for their proximate composition. RBPC was analyzed for its bulk density, foaming capacity (FC), emulsion activity (EA), and water and oil absorption capacity. RBPC was incorporated in biscuits at 5, 10 and 15 per cent levels. The biscuits were analyzed for their physicochemical, fracture strength (texture analyzer) and sensory attributes (nine‐point hedonic scale) to assess their acceptability.FindingsThe maximum yield of 13.2 per cent for RBPC was obtained at alkaline pH of 11, temperature of 60°C and extraction time of 60 minutes. The bulk density, foaming capacity, emulsifying ability, water and oil absorption capacity of RBPC were 0.4 g/ml, 11 per cent, 40 per cent, 2.9 ml/g and 2.3 ml/g respectively. The protein content of biscuits increased significantly from 7.3 per cent in control biscuits to 15.4 per cent in the 15 per cent RBPC supplemented biscuits with their fracture strength also significantly higher than the control biscuits (p<0.05). Replacement of refined wheat flour up to 10 per cent RBPC produced protein‐enriched biscuits with desirable overall acceptability.Originality/valueRice bran protein concentrate can be beneficially utilized to formulate protein enriched biscuits with enhanced nutritional value especially for malnourished or undernourished people.