Albopictus Mosquitoes Research Articles

Hubungan Status Gizi Dan Praktik 3M Dengan Kejadian DBD Di Kota Bengkulu Tahun 2022

Dengue Hemorrhagic Fever (DHF) or Dengue Haemorrhagic Fever (DHF), commonly called Dengue Haemorrhagic Fever (DHF), is a disease caused by the dengue virus, which is transmitted by Aedes Aegypti and Aedes Albopictus mosquitoes. The World Health Organization (WHO) states that the number of reported cases of dengue fever has increased more than eightfold over the last four years, from 505,000 cases in 2013 to 4.2 million in 2019. Based on data from the Ministry of Health for 2020, cases of dengue hemorrhagic fever (DHF) in Indonesia reached 71,700 cases. According to data from the Bengkulu Provincial Health Office, the incidence of DHF in Bengkulu Province is relatively high, with morbidity and mortality rates above the national target. There were 1,261 cases of DHF that occurred in Bengkulu Province in 2020 (Bengkulu Provincial Health Office, 2020). This research is a quantitative descriptive analytic research with a case-control design, namely an analytic observation research conducted by comparing the case group and the control group based on their exposure status (retrospective). This design moves from the effect (illness) to the cause (exposure). aims to determine whether there is a relationship between nutritional status and 3M practises and the incidence of DHF. The results of this study show that there is a relationship between nutritional status and the incidence of DHF in Bengkulu City in 2022, and there is a relationship between 3M practises and the incidence of DHF in Bengkulu City in 2022.

The Relationship of Parents Knowledge with DHF Prevention Behavior in Children's Room, Santo Vincentius Hospital Singkawang

DHF (Dengue Hemorrhagic Fever) is a disease caused by Aedes Aegepty and Aedes Albopictus mosquitoes that can appear throughtout the year, and can attack all age groups, expecially children. Individual behavior in preventing Dengue fever depends on knowledge, which can bring real action for individuals to prevent DHF. The study aims to determine the relationship between parents knowledge level and DHF prevention behavior in the childreen’s room at St. Vincentius hospital Singkawang. The study was conducted in Agustus 2022 with a cross sectional design use in a study of 30 respondens whose childreen where in a emergency in pediatric ward of at.St. Vincentius Hospital because of DHF case. The level of knowledge of parent was measured by a Guttman scale questionnaire and for DHF prevention behavior using aquestionnaire with a Likert scale. The result of statistical test using fisher extract test on chi square obtained p-value 0,039 < 0,05, so that H0 rejected , which mean that there is a relationship between the level of parental knowledge and the prevention behavior of DHF in the Childreen’s room, St.Vincentius Hospital Singkawang. Parental knowledge about DHF desease greatly influences parental behavior in preventing DHF. Based on research that behavior based and knowledge will be more durable than behavior that is not based on knowledge people will prevent DHF transmission if they know what the goal and benefits are for health. Thus, the suggestion for the Santo Vincentius Hospital is to increase further counseling related to the prevention of DHF both in the hospital environtment and in the community arround the hospital in collaboration with the local government.

Nanomechanical and Vibrational Signature of Chikungunya Viral Particles.

Chikungunya virus (CHIKV) belongs to the genus Alphaviridae, with a single-stranded positive-sense RNA genome of 11.8 kbp encoding a polyprotein that generates both non-structural proteins and structural proteins. The virus is transmitted by the Aedes aegypti and A. albopictus mosquitoes, depending on the location. CHIKV infection leads to dengue-like musculoskeletal symptoms and has been responsible for several outbreaks worldwide since its discovery in 1952. Patients often experience fever, headache, muscle pain, joint swelling, and skin rashes. However, the ultrastructural and mechanical properties of CHIKV have not been fully characterized. Thus, this study aims to apply a physical approach to investigate CHIKV's ultrastructural morphology and mechanical properties, using atomic force microscopy and Raman spectroscopy as the main tools. Using nanomechanical assays of AFM and a gold nanoparticles substrate for Raman signal enhancement, we explored the conformational plasticity, morphology, vibrational signature, and nanomechanical properties of the chikungunya virus, providing new information on its ultrastructure at the nanoscale and offering a novel understanding of the virus' behavior upon mechanical disruptions besides its molecular composition.

The effects of climate factors, population density, and vector density on the incidence of dengue hemorrhagic fever in South Jakarta Administrative City 2016-2020: an ecological study.

Dengue hemorrhagic fever (DHF) is an infectious disease caused by the dengue virus (DENV) and is transmitted through the bite of the Aedes aegypti and Aedes albopictus mosquitoes. This study aims to analyze the relationship between the incidence of DHF which can be influenced by climatic factors in the same month (non-time lag), climatic factors with a lag of 1 month (time lag 1), climatic factors with a lag of 2 months (time lag 2), population density, and vector density. The study design used is an ecological study. The data is sourced from the South Jakarta City Administration of Health, the South Jakarta City Administration of Central Statistics, and the Meteorology, Climatology and Geophysics Agency. Data were analyzed using correlation test. The results showed that the incidence of DHF was related to non-time lag rainfall, time lag 1, and time lag 2, air temperature time lag 2, air humidity non-time lag, time lag 1, and time lag 2, population density, and numbers of mosquito's larvae free index (ABJ). DHF is still a disease that needs to be watched out for in the South Jakarta Administrative City, requiring the government and the people of the South Jakarta Administration to continue to increase efforts to prevent and control DHF.

Absence of transmission of vYF next generation Yellow Fever vaccine in mosquitoes.

One of the most effective vaccines against an arbovirus is the YFV-17D live-attenuated vaccine developed in 1937 against Yellow Fever (YF). This vaccine replicates poorly in mosquitoes and consequently, is not transmitted by vectors. Vaccine shortages, mainly due to constrained productions based on pathogen-free embryonated eggs, led Sanofi to move towards alternative methods based on a state-of-the-art process using continuous cell line cultures in bioreactor. vYF-247 is a next-generation live-attenuated vaccine candidate based on 17D adapted to grow in serum-free Vero cells. For the development of a new vaccine, WHO recommends to document infectivity and replication in mosquitoes. Here we infected Aedes aegypti and Aedes albopictus mosquitoes with vYF-247 vaccine compared first to the YF-17D-204 reference Sanofi vaccines (Stamaril and YF-VAX) and a clinical human isolate S-79, provided in a blood meal at a titer of 6.5 Log ffu/mL and secondly, to the clinical isolate only at an increased titer of 7.5 Log ffu/mL. At different days post-infection, virus replication, dissemination and transmission were evaluated by quantifying viral particles in mosquito abdomen, head and thorax or saliva, respectively. Although comparison of vYF-247 to reference vaccines could not be completed to yield significant results, we showed that vYF-247 was not transmitted by both Aedes species, either laboratory strains or field-collected populations, compared to clinical strain S-79 at the highest inoculation dose. Combined with the undetectable to low level viremia detected in vaccinees, transmission of the vYF-247 vaccine by mosquitoes is highly unlikely.

Predicting the Effects of Climate Change on Dengue Vector Densities in Southeast Asia through Process-Based Modeling.

Aedes aegypti and Ae. albopictus mosquitoes are major vectors for several human diseases of global importance, such as dengue and yellow fever. Their life cycles and hosted arboviruses are climate sensitive and thus expected to be impacted by climate change. Most studies investigating climate change impacts on Aedes at global or continental scales focused on their future global distribution changes, whereas a single study focused on its effects on Ae. aegypti densities regionally. A process-based approach was used to model densities of Ae. aegypti and Ae. albopictus and their potential evolution with climate change using a panel of nine CMIP6 climate models and climate scenarios ranging from strong to low mitigation measures at the Southeast Asian scale and for the next 80 y. The process-based model described, through a system of ordinary differential equations, the variations of mosquito densities in 10 compartments, corresponding to 10 different stages of mosquito life cycle, in response to temperature and precipitation variations. Local field data were used to validate model outputs. We show that both species densities will globally increase due to future temperature increases. In Southeast Asia by the end of the century, Ae. aegypti densities are expected to increase from 25% with climate mitigation measures to 46% without; Ae. albopictus densities are expected to increase from 13%-21%, respectively. However, we find spatially contrasted responses at the seasonal scales with a significant decrease in Ae. albopictus densities in lowlands during summer in the future. These results contrast with previous results, which brings new insight on the future impacts of climate change on Aedes densities. Major sources of uncertainties, such as mosquito model parametrization and climate model uncertainties, were addressed to explore the limits of such modeling. https://doi.org/10.1289/EHP11068.

Lactic acid bacteria waste infusion as a source of attraction and oviposition stimulation of gravid female Aedes albopictus mosquitoes.

Aedes albopictus poses a public health risk in tropical countries and temperate countries in recent decades due to its capability to transmit various human arboviruses including dengue, yellow fever, and chikungunya. Vector control is the key for preventing transmission of these pathogenic viruses. Improving the effectiveness of currently utilized collection methods, such as ovitraps, is important for best species abundance monitoring, assessment of the threat of arbovirus transmission, and optimizing control activities. Therefore, this study aimed to assess the potential use of lactic acid bacteria (LAB) waste as an infusion-baited ovitrap for Aedes collection. The performance of overnight tap water, grass hay infusion and LAB waste infusion were compared for their ability in attracting gravid female Ae. albopictus. In this study, the LAB waste infusion was substantially more alluring to Ae. albopictus mosquitoes than the two controls grass hay infusion and tap water.

Surveillance of Aedes populations in Jiangsu Province in 2020

To investigate the seasonal Aedes population fluctuation and the resistance of Aedes populations to common insecticides in Jiangsu Province in 2020, so as to provide insights into vector-borne infectious diseases control. One village was randomly sampled from each of Xinbei District of Changzhou City and Zhangjiagang County of Suzhou City in southern Jiangsu Province, Hai'an County of Nantong City and Yandu District of Yancheng City in Central Jiangsu Province, and Suining County of Xuzhou City and Sihong County of Suqian City in northern Jiangsu Province during the period between May and October, 2020. A small ponding container was sampled, and larval Aedes mosquitoes were collected using straws once each in early and late stages of each month. All larvae were bred in laboratory to adults for population identification. In addition, larval breeding were observed in all small ponding containers in and out of 30 households that were randomly sampled from six surveillance sites, and the larval mosquito density was estimated using Breteau index. Larval A. albopictus mosquitoes were sampled around Cuiyuan New Village in Jintan District of Changzhou City, and bred in laboratory to the first offspring generation, and the susceptibility of adult female mosquitoes to deltamethrin, lambda-cyhalothrin, malathion, and propoxur was tested using the filter-paper bioassay recommended by WHO. A total of 1 165 larval Aedes mosquitoes were captured from small ponding containers in six surveillance sites of Jiangsu Province in 2020, and all were identified as A. albopictus following eclosion. The largest number of Aedes larvae captured was found in July. A total of 1 152 households were investigated in six surveillance sites, and the mean Breteau indexes were 9.58, 13.20, 13.71, 13.20, 12.18 and 5.58 from May to October, respectively, while a high Aedes transmission risk was seen in Xinbei District of Changzhou City, with a higher Breteau index than in Suining (H = 23.667, Padjusted = 0.001) and Sihong (H = 22.500, Padjusted = 0.003) counties. The field-captured A. albopictus from Cuiyuan New Village in Jintan District of Changzhou City remained sensitive to malathion, but was resistant to propoxur, and developed high-level resistance to deltamethrin and lambda-cyhalothrin. A. albopictus was present in southern, central and northern Jiangsu Province in 2020, and the larval density peaked in July. A. albopictus captured from Cuiyuan New Village in Jintan District of Changzhou City has developed high-level resistance to pyrethroid pesticides.

The Potential of a New Beauveria bassiana Isolate for Mosquito Larval Control.

The African malaria mosquito, Anopheles gambiae Giles (Diptera: Culicidae), and the Asian tiger mosquito, Aedes albopictus Skuse (Diptera: Culicidae) are of public health concern due to their ability to transmit disease-causing parasites and pathogens. Current mosquito control strategies to prevent vector-borne diseases rely mainly on the use of chemicals. However, insecticide resistance in mosquito populations necessitates alternative control measures, including biologicals such as entomopathogenic fungi. Here we report the impact of a new Beauveria bassiana (Balsamo) Vuillemin (Hyprocreales: Cordycipitaeceae) isolate, isolated from field-collected Ae. albopictus larvae on mosquito survival and development. Larval infection bioassays using three B. bassiana conidial concentrations were performed on the second and third larval instars of An. gambiae and Ae. albopictus mosquitoes. Larvae were monitored daily for survival and development to pupae and adults. Our results show that B. bassiana MHK was more effective in killing An. gambiae than Ae. albopictus larvae. We further observed delays in development to pupae and adults in both mosquito species exposed the varying concentrations of B. bassiana as compared to the water control. In addition, larval exposure to B. bassiana reduced adult male and female survival in both mosquito species, further contributing to mosquito population control. Thus, this study identifies a new B. bassiana isolate as a possible biological control agent of two mosquito species of public health concern, increasing the arsenal for integrated mosquito control.

Virome and nrEVEome diversity of Aedes albopictus mosquitoes from La Reunion Island and China

BackgroundAedes albopictus is a public health threat for its worldwide spread and ability to transmit arboviruses. Understanding mechanisms of mosquito immunity can provide new tools to control arbovirus spread. The genomes of Aedes mosquitoes contain hundreds of nonretroviral endogenous viral elements (nrEVEs), which are enriched in piRNA clusters and produce piRNAs, with the potential to target cognate viruses. Recently, one nrEVE was shown to limit cognate viral infection through nrEVE-derived piRNAs. These findings suggest that nrEVEs constitute an archive of past viral infection and that the landscape of viral integrations may be variable across populations depending on their viral exposure.MethodsWe used bioinformatics and molecular approaches to identify known and novel (i.e. absent in the reference genome) viral integrations in the genome of wild collected Aedes albopictus mosquitoes and characterize their virome.ResultsWe showed that the landscape of viral integrations is dynamic with seven novel viral integrations being characterized, but does not correlate with the virome, which includes both viral species known and unknown to infect mosquitoes. However, the small RNA coverage profile of nrEVEs and the viral genomic contigs we identified confirmed an interaction among these elements and the piRNA and siRNA pathways in mosquitoes.ConclusionsMosquitoes nrEVEs have been recently described as a new form of heritable, sequence-specific mechanism of antiviral immunity. Our results contribute to understanding the dynamic distribution of nrEVEs in the genomes of wild Ae. albopictus and their interaction with mosquito viruses.

Optimization of adult mosquito trap settings to monitor populations of Aedes and Culex mosquitoes, vectors of arboviruses in La Reunion

Competent arbovirus vectors are found in the culicid mosquito fauna of south-west Indian Ocean (SWIO) islands. In La Reunion, Aedes albopictus and Aedes aegypti mosquitoes are known vectors of dengue and chikungunya viruses. Culex quinquefasciatus is a potential vector of Rift Valley fever and West Nile viruses. To prepare a vector-control field trial against Ae. aegypti, this study aimed at identifying the best trapping strategy to catch adult Ae. aegypti, using BG-Sentinel traps (Biogents, Germany). It was implemented in two sites in southern La Reunion. Catches of Ae. albopictus and Cx. quinquefasciatus mosquitoes were also recorded. A Latin square design was used to estimate the detection probability and the apparent daily density—according to the BG-Sentinel trapping strategy: none, carbon dioxide (CO2), a commercial attractant—BG-Lure (Biogents, Germany), or both. The use of CO2 alone was associated with a higher detection probability for Ae. aegypti and Cx. quinquefasciatus mosquitoes, as well as a large increase in their apparent density. Traps with BG-Lure—alone or in combination with CO2, did not improve the detection probability of Ae. aegypti and Cx. quinquefasciatus mosquitoes. The same result was found for male Ae. albopictus. For females, baiting BG-Sentinel traps with CO2 or BG-Lure had no significant effect. The same apparent densities were found for Ae. aegypti and Ae. albopictus mosquitoes in both study sites—where Ae. aegypti mosquitoes were found at very low densities during previous surveys.

To estimate the prevailing Serotype of dengue virus and IgM antibody in suspected dengue fever cases

Dengue virus infection a vector borne disease affecting more than 50 million people worldwide annually. It is distributed in tropical and subtropical regions of the world, abundant in urban and semi-urban areas. It is the disease that spreads by bite of Aedes aegypti and Aedes albopictus mosquitoes. The virus being a RNA virus belongs to Flaviviridae family. The four serotypes of Dengue virus are DENV-1, DENV-2, DENV-3, and DENV-4 which have distinct antigenicity complex.This study was conducted in Microbiology Department, Government Stanley Medical College, Chennai, during the period Jan 2018 to Dec 2018 with 120 blood samples from patients of suspected dengue fever. Samples were selected by stratified random sampling method into three strata and each strata consisting of 40 samples based on duration of fever. All the samples were subjected to procedure for both NS1 antigen and IgM antibodies.15 NS1 positives samples with duration of fever less than 5 days were serotyped by PCR. : A total of 18 cases had NS1 antigen, 26 cases had IgM antibodies and 27 cases had both. A combination of the two tests detected a total of 44 cases. The chance of detection of NS1antigen decreased with duration of fever and IgM antibodies increased with duration of fever. The serotyping detected were DENV1 and DENV3 NS1 antigen detection by ELISA is useful in early phase of infection. A combination of NS1 ELISA and IgM ELISA have high sensitivity depending upon the duration of illness. Detection of DENV1 and DENV3 by PCR procedure helps to know the presence of prevailing serotype in this particular region which would be a good guide to predict the recurrence of outbreak and for efficient prevention and management.

Genome-wide SNPs reveal novel patterns of spatial genetic structure in Aedes albopictus (Diptera Culicidae) population in China.

Since the second half of the 20th century, Aedes albopictus, a vector for more than 20 arboviruses, has spread worldwide. Aedes albopictus is the main vector of infectious diseases transmitted by Aedes mosquitoes in China, and it has caused concerns regarding public health. A comprehensive understanding of the spatial genetic structure of this vector species at a genomic level is essential for effective vector control and the prevention of vector-borne diseases. During 2016-2018, adult female Ae. albopictus mosquitoes were collected from eight different geographical locations across China. Restriction site-associated DNA sequencing (RAD-seq) was used for high-throughput identification of single nucleotide polymorphisms (SNPs) and genotyping of the Ae. albopictus population. The spatial genetic structure was analyzed and compared to those exhibited by mitochondrial cytochrome c oxidase subunit 1 (cox1) and microsatellites in the Ae. albopictus population. A total of 9,103 genome-wide SNP loci in 101 specimens and 32 haplotypes of cox1 in 231 specimens were identified in the samples from eight locations in China. Principal component analysis revealed that samples from Lingshui and Zhanjiang were more genetically different than those from the other locations. The SNPs provided a better resolution and stronger signals for novel spatial population genetic structures than those from the cox1 data and a set of previously genotyped microsatellites. The fixation indexes from the SNP dataset showed shallow but significant genetic differentiation in the population. The Mantel test indicated a positive correlation between genetic distance and geographical distance. However, the asymmetric gene flow was detected among the populations, and it was higher from south to north and west to east than in the opposite directions. The genome-wide SNPs revealed seven gene pools and fine spatial genetic structure of the Ae. albopictus population in China. The RAD-seq approach has great potential to increase our understanding of the spatial dynamics of population spread and establishment, which will help us to design new strategies for controlling vectors and mosquito-borne diseases.

Children’s Hands Washing Activities in the Prevention of COVID-19

One of the infectious diseases that have high morbidity and mortality rates is Dengue Hemorrhagic Fever (DHF) which is transmitted through the bite of Aedes aegypti and Aedes albopictus mosquitoes. In West Java Province, dengue cases fluctuated. So it is necessary to analyze and review the influence of weather on dengue cases in West Java Province with the pattern and prevalence of dengue cases in all cities and districts in West Java Province. This type of research is quantitative descriptive research by making exploratory observations and producing something general with a Geographic Information System approach. The location of this research is in all 27 districts and cities in West Java Province. Data on dengue cases in West Java Province is accessed from the West Java Provincial Health Office on the West Java Open Data platform (https://opendata.jabarprov.go.id/). Then the population data of West Java Province can be accessed at the Central Statistics Agency of West Java Province on the https://jabar.bps.go.id/ platform. Analysis of this research data for incidence is calculated by dividing the number of cases divided by the population and then multiplying by one hundred thousand, while the prevalence can be calculated by dividing the number of cases divided by the population and then multiplying by a thousand, and mapping using QGIS 3.16. From 2014 – 2020, the highest cases were in the city of Bandung and the highest prevalence was in the city of Sukabumi. there is a relationship between rainfall and DHF cases in West Java Province in 2014 - 2020 with a moderate correlation strength where rainfall plays a role in the incidence of DHF cases but is not absolute because it can still be influenced by other factors.

Initial Recognition and Attachment of the Zika Virus to Host Cells: A Molecular Dynamics and Quantum Interaction Approach.

The zika virus (ZIKV), transmitted to humans from the bites of Aedes Aegypti and Aedes Albopictus mosquitoes produces Zika fever and neurodegenerative disorders that despite affecting millions of people, most recently in Africa and the Americas, has been declared a neglected tropical disease by the World Health Organization. In this work, atomistic molecular dynamics simulations followed by rigorous analysis of the intermolecular interactions reveal crucial aspects of the initial virus⋯cell molecular recognition and attachment, events that trigger the infectious cycle. Previous experimental studies have shown that Dermatan Sulfate (DS) and Chondroitin Sulfate A (CSA), two glycosaminoglycans which are actually epimers to each other and that are structural constituents of receptors expressed in cell membranes, are the preferred anchorage sites, with a marked preference for DS. Our calculations rationalize this preference from a molecular perspective as follows: when free of the virus, DS has one sulfate group that does not participate in intramolecular strong hydrogen bonds, thus, it is readily available to interact with the envelope protein of the virus (Zika-E), then, after formation of the complexes, Zika-E⋯DS exhibits ten strong salt brides connecting the two fragments against only six salt bridges and two hydrogen bonds in Zika-E⋯CSA. Our results complement the current view of the interaction between the virus and the receptor glycosoaminoglycans revealing that the negatively charged carboxylate groups in CSA and DS are just as important as the sulfates because of the formation of equally strong salt bridges with the positively charged Arginine and Lysine aminoacids in the envelope protein of the virus.

Clinical Presentation and Platelet Profile of Dengue Fever: A Retrospective Study.

Background: Dengue fever (DF) is a mosquito-borne viral illness carried worldwide by Aedes aegypti and Aedes albopictus mosquitoes. The aim of the present study was to observe the different clinical presentations of dengue fever and the platelet profile analysis in DF patients.Methods: This retrospective study was performed on 130 diagnosed patients with dengue fever, aged over 14 years. Data collection included patient age, gender, clinical manifestations, hematocrit, platelet count, and evidence of plasma leakage.Results: Most of the patients belong to the 21-30 year age group, with a greater number of males compared to females. The common presentation of dengue fever was fever and myalgia, observed in 100% and 95.3% of the patients, respectively. A platelet count of less than 1,00,000 was observed in 77% of patients, whereas decreased total leukocyte count (TLC) and hematocrit were observed in 52.3% and 40% of patients, respectively. About 46.15% of patients had bradycardia on examination. Pleural effusion and ascites were found in 20.7% and 15.3% of patients, respectively.Conclusion: Patients presenting with fever, hemorrhagic symptoms, or signs of plasma leakage should be promptly suspected, timely diagnosed and managed on the grounds of dengue fever.

SCG Mosquito Trap: A New Lethal Ovitrap for Controlling Aedes aegypti (L.) and Ae. albopictus (Skuse); the Vectors of Dengue Haemorrhagic Fever, and Chikungunya and Zika Fever

Aedes aegypti (L.) and Ae. albopictus (Skuse) are known to be the vectors of dengue haemorrhagic fever (DHF), and chikungunya and zika fever. Of these diseases, DHF is the most dangerous because it has high incidence and mortality in many countries worldwide, especially in Asia. As there is currently no effective vaccine available to prevent these diseases, vector control is the best strategy for controlling them. Therefore, the SCG Mosquito Trap: a new lethal ovitrap combined with attractant and biological larvicide, was developed in this study for controlling Ae. aegypti and Ae. albopictus mosquitoes. The trap without attractant showed significantly higher oviposition efficacy than that of a conventional one in luring gravid Ae. aegypti and Ae. Albopictus to lay their eggs under laboratory conditions by approximately 3.1 and 2.6 times, respectively. The attractant derived from carpet shell extract increased the oviposition efficacy of the SCG Mosquito Trap by about 1.7 and 4.1 times against Ae. aegypti and Ae. albopictus, respectively, under laboratory conditions. Bacillus thuringiensis israelensis (Bti) was the biological larvicide used in the SCG Mosquito Trap. It provided a complete killing effect (100% mortality) against the larvae of Ae. aegypti and Ae. albopictus for 4 weeks post single application, as no larvae hatched from sponge sheets applied with Bti, which were obtained from the SCG Mosquito Trap. In the field study against Ae. aegypti, the SCG Mosquito Trap could reduce the number of houses infested from 80% (with 75% from the positive trap and an average of 242 eggs/trap) to 30% (with 22.5% from the positive trap and an average of 64 eggs/trap) by the fourth week after initiation. In conclusion, the SCG Mosquito Trap developed from this study could be used additionally as an effective device for controlling Ae. aegypti and Ae. albopictus mosquitoes, in order to prevent the diseases that are transmitted by these vectors.  &nbsp

The origin and continuing adaptive evolution of chikungunya virus.

Chikungunya virus (CHIKV) is the responsible agent of chikungunya fever, a debilitating arthritic disease in humans. CHIKV is endemic in Africa and Asia, although transmission cycles are considerably different on these continents. Before 2004, CHIKV had received little attention, since it was only known to cause localised outbreaks in a limited region with no fatalities. However, the recent global reemergence of CHIKV has caused serious global health problems and shown its potential to become a significant viral threat in the future. Unexpectedly, the reemergence is more rapid and is geographically more extensive, especially due to increased intensity of global travel systems or failure to contain mosquito populations. Another important factor is the successful adaptation of CHIKV to a new vector, the Aedes albopictus mosquito. Ae. albopictus survives in both temperate and tropical climates, thus facilitating CHIKV expansion to non-endemic regions. The continuous spread and transmission of CHIKV pose challenges for the development of effective vaccines and specific antiviral therapies. In this review, we discuss the biology and origin of CHIKV in Africa as well as its subsequent expansion to other parts of the world. We also review the transmission cycle of CHIKV and its continuing adaptation to its mosquito vectors and vertebrate hosts. More-complete understanding of the continuous evolution of CHIKV may help in predicting the emergence of CHIKV strains with possibly greater transmission efficiency in the future.

A Mark-Release-Recapture Study to Estimate Field Performance of Imported Radio-Sterilized Male Aedes albopictus in Albania.

The pathogen transmitting Aedes albopictus mosquito is spreading rapidly in Europe, putting millions of humans and animals at risk. This species is well-established in Albania since its first detection in 1979. The sterile insect technique (SIT) is increasingly gaining momentum worldwide as a component of area-wide-integrated pest management. However, estimating how the sterile males will perform in the field and the size of target populations is crucial for better decision-making, designing and elaborating appropriate SIT pilot trials, and subsequent large-scale release strategies. A mark-release-recapture (MRR) experiment was carried out in Albania within a highly urbanized area in the city of Tirana. The radio-sterilized adults of Ae. albopictus Albania strain males were transported by plane from Centro Agricoltura Ambiente (CAA) mass-production facility (Bologna, Italy), where they were reared. In Albania, sterile males were sugar-fed, marked with fluorescent powder, and released. The aim of this study was to estimate, under field conditions, their dispersal capacity, probability of daily survival and competitiveness, and the size of the target population. In addition, two adult mosquito collection methods were also evaluated: BG-Sentinel traps baited with BG-Lure and CO2, (BGS) versus human landing catch (HLC). The overall recapture rates did not differ significantly between the two methods (2.36% and 1.57% of the total male released were recaptured respectively by BGS and HLC), suggesting a similar trapping efficiency under these conditions. Sterile males traveled a mean distance of 93.85 ± 42.58 m and dispersed up to 258 m. Moreover, they were observed living in the field up to 15 days after release with an average life expectancy of 4.26 ± 0.80 days. Whether mosquitoes were marked with green, blue, yellow, or pink, released at 3.00 p.m. or 6.00 p.m., there was no significant difference in the recapture, dispersal, and survival rates in the field. The Fried competitiveness index was estimated at 0.28. This mark-release-recapture study provided important data for better decision-making and planning before moving to pilot SIT trials in Albania. Moreover, it also showed that both BG-traps and HLC were successful in monitoring adult mosquitoes and provided similar estimations of the main entomological parameters needed.

Relationship Of Knowledge On Community Behavior In Eradication Of Aedes Aegypti Mosquito Nests In The Work Area Of Pal. 3 Puskesmas Pontianak

Dengue Hemorrhagic Fever (DHF) is transmitted through the bite of the Aedes aegypti mosquito and possibly the Aedes albopictus mosquito. The incidence of DHF in Indonesia in the last 3 years of 2018 was 65,602 cases, in 2019 as many as 138,127 cases, and in 2020 as many as 108,303 cases. In West Kalimantan, in 2018, as many as 3,125 cases, in 2019 as many as 2,798 cases, and in 2020 as many as 784 cases. Meanwhile, data on dengue cases in Pontianak City, in 2018 there were as many as 182 cases, in 2019 there were as many as 106 cases, and in 2020 there were as many as 27 cases. Nationally, cases of DHF fluctuate, while in West Kalimantan to Pontianak, it tends to decrease. The death rate (CFR) due to DHF cases in Pontianak City during the period 2018-2019 showed the highest mortality rate occurred in 2018 with a percentage of 1,54% of 100 DHF cases. However, the incidence rate is still there, and every year (endemic) and always has the potential to cause outbreaks. Therefore, efforts to control mosquitoes carrying dengue disease are needed, starting by assessing community knowledge and behavior in controlling DHF disease with nest eradication. Mosquitoes (PSN 3M plus). Objective To examine the relationship between knowledge and community behavior in PSN 3M plus in the work area of ​​the Pal.3 Pontianak Health Center. Methodology is observational research with a cross-sectional approach. The results of the study showed that there was a significant relationship between knowledge and PSN 3M plus in the work area of ​​the Pontianak Pal.3 Health Center, with a value of p = 0.000 (p<). Most respondents have poor knowledge in PSN 3M plus (61,7%), as well as bad behavior in PSN 3M plus (77,7%), so it is concluded that there is a significant relationship between respondents' knowledge and behavior in PSN 3M Plus in the work area of ​​the Pontianak Pal. 3 Health Center.