In accordance with the airworthiness standards, the aircraft structure must be operationally survivable, it means that structure must be able to remain efficiency in the presence of admissible damage. But, accumulating above than a certain level, damages cause fatigue failure of the structure, in the form of micro- and submicrocracks, thus reducing its strength characteristics. Currently, several approaches have been formed to ensure the safety of an aircraft structures in terms of strength. One of them is ensuring a safe resource (safe durability). This principle implies that during the specified service life of the product, no damage will occur in it, reducing the strength below the permissible level. The aircraft resource is limited “from above” by the durability of the regular zones of airframe. Therefore, predicting the durability of an aircraft wing structure at the design stage is a fundamental engineering problem to ensure its safety and economic efficiency. At the same time, the first step in dealing with aircraft fatigue damage at the design stage is the collection and assessment of the operational loads of the analog aircraft. However, at the design stage of a new aircraft model, obtaining such data is not always possible. Therefore, the purpose of this article is to develop a method for calculating fatigue damage at the stage of cracking and assessing the durability of regular zones of a transport aircraft wing, taking into account the conditions of its operation. The tasks to be solved are: to isolate the factors that determine the durability of the aircraft at flying in turbulent air; to take into account the asymmetry of loads and accumulated damage that occurs at each stage during the entire flight of the aircraft; to determine the aircraft's resource depending on the profile of a typical flight. The method is based on a standardized atmospheric turbulence model, typical flight profiles, fatigue characteristics of materials, the hypothesis of linear summation of damages and calculation based on nominal stresses. As result, comparison between the calculated integral repeatability of overloads and equivalent bending moments with the results of processing flight test data showed good agreements. Conclusions. The scientific novelty of the work lies in the fact that a method for calculating the fatigue damage of the regular wing zones, taking into account the expected flight profile of the aircraft was developed. This means that the proposed method makes it possible to carry out a preliminary assessment of the resource when designing an airplane without using data on the operational loads of an analogue airplane, and also estimate the residual resource of the airplane during its operation.