The ventilation efficiency of different ventilation patterns arranged by two inlet and two outlet diffusers at different locations is investigated. The numerical simulation was first adopted to predict airflow pattern and ventilation performance. Then, a laboratory experiment at room air and contaminant distribution in a full-scale test chamber was conducted to validate the simulation results. Applied the tracer-gas techniques to the experimental program, a concentration decay of CO 2 is used to calculate the ventilation efficiency and air change rate of the test chamber. The distribution of supply/exhaust air in the study characterized by ventilation efficiency, was investigated in isothermal conditions using different supply air velocities. The results indicate that the ventilation efficiency might be dominantly influenced by location of diffuser rather than air change rate.