Received: 2016-11-23 | Accepted: 2016-04-14 | Available online: 2016-12-22 http://dx.doi.org/10.15414/afz.2016.19.04.157-162 The objective of this research was to investigate possible genetic variation in the sensitivity of soybean cultivars for nitrogen fixation rates in response to soil drying. The work confirmed that the selected physiological characteristics (RWC, osmotic potential, stress index and created nodules on roots) are good evaluating parameters for the determination of water stress in plant. In the floricultural year 2014 an experiment with four genetic resources of soybean was launched. Sowing of Maverick (USA), Drina (HRV), Nigra (SVK) and Polanka (CZK) genotypes was carried out in the containers of 15 l capacity. This stress had a negative impact on the physiological parameters. By comparing the RWC values, the decrease was more significant at the end of dehydration, which was monitored in Maverick and Drina genotypes using the Nitrazon inoculants and water stress effect. Inoculated stressed Nigra and Polanka genotypes have kept higher water content till the end of dehydration period. Also the proline accumulation was monitored during the water stress, whilst higher content of free proline reached of Maverick. More remarkable decrease of osmotic potential was again registered in a foreign Drina and Maverick genotypes in the inoculated variations. Nigra and Polanka genotypes responses not so significant in the given conditions. Key words: soybean, drought, stress, inoculation of Nitrazon References CANDRAKOVA, E. and LISKA, E. (2006) Vplyv teplotných a vlahových podmienok na tvorbu urody hrachu siateho, Influence of Temperature and Water Conditions on Field Pea Yield Formation. Acta fytotechnica et zootechnica, vol. 9, no. 3, pp. 57-61. DOWNIE, J. A. 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