Agricultural knowledge and innovation system (AKIS) has a strong potential to enhance economic performance of farming and contribute to agricultural sustainability, as it may increase synergies and complementarity among actors. This paper is aimed to develop a proposed framework to strengthen AKIS in the study area based on the results of this study. This paper explores perception and views about strengthening AKIS in Dakhalia governorate of Egypt by applying a multi-actor approach. Quantitative and qualitative data were collected through face-to-face interviews and focus group discussion. This paper focuses on three structural dimensions, namely actors, interactions, and technologies, to describe the nature of innovation processes within AKIS. Results indicate that legal and regulatory frameworks, lack of infrastructure, and weak the role of intermediary organizations are the main barriers that AKIS faces. Linkages of contracting and public–private partnerships were viewed as main interactions required to strengthen AKIS. The analysis also explores the availability of innovation requirements at each actor, as well as the distributive technologies, that should be encouraged to build the capacity the agricultural sector. A proposed framework is developed based on the results of this study and the characteristics of the AKIS in the study area. This framework could be used for stimulating innovation and enhancing coordination between actors.