Aging Process Research Articles

Insights into the Deterioration Mechanism of Charging Ability during Calendar Aging and Cycling Aging of High-Voltage Co-Poor NCM Cathode-Graphite Full Battery.

Lithium-ion battery (LIB) has gained significant recognition for the power cell market owing to its impressive energy density and appealing cost benefit. Among various cathodes, a high-voltage cobalt-poor lithium nickel manganese cobalt oxide cathode (Co-poor NCM cathode) has been considered as a promising strategy to enhance its energy density. Despite these advantages, high-voltage Co-poor NCM cathode-graphite full battery usually suffers from poor rate performance. However, fast charging has been a key indicator for widespread application of power batteries. Although many efforts have been made to improve the charging performance of fresh batteries, few works investigate the charging ability during calendar aging and cycling aging of high-voltage Co-poor NCM cathode-graphite full battery. In this work, we found that the charging ability becomes worse during calendar aging and cycling aging. Results showed that the increasing charge transfer resistance from the cathode is the major obstacle to achieving fast charging during the aging process. To address the problem, high-voltage Al2O3-coated Co-poor NCM cathode successfully prepared via a simple atomic layer deposition (ALD) method has been developed to reduce the decay of charging performance during calendar aging and cycling aging. Al2O3-coated NCM cathode can effectively reduce the growth rate of the resistance of cathode, which is benefiting from the conversion of Al2O3 into LiAlO2 with high ionic conductivity and the restriction formation of rock salt phase. Benefiting from the decreased charge transfer resistance of the NCM cathode, the mismatch of the lithium-ion reaction kinetics is well alleviated, thus effectively reducing the polarization under fast charging. As a result, Al2O3-coated NCM cathode-graphite full battery shows the slow deterioration of charging performance during the aging process. This work provides a promising strategy for constructing fast-charging batteries during calendar aging and cycling aging.

Single-cell analysis reveals alternations between the aged and young mice prostates

BackgroundAging of the male prostate is an inevitable process in which the prostate undergoes hyperplasia, and this growth may lead to compression of the urethra, resulting in voiding dysfunction and associated symptoms, and an increased risk of prostate cancer. Despite the significance of prostate aging, the molecular mechanisms involved are still not fully understood.MethodsProstate split by lobes from young (2 months) and aged (24 months) mice were collected for single-cell RNA sequencing (scRNA-seq) analysis. Tissues from both anterior prostate (AP) and ventral/dorsal/lateral prostate (VDLP) were included in the study. Data analysis included unsupervised clustering using the uniform manifold approximation and projection (UMAP) algorithm to identify distinct cell types based on marker gene expression. Differential gene expression analysis was performed to identify age-related changes in gene expression across different cell types. Functional enrichment analysis was conducted to elucidate biological pathways associated with differentially expressed genes. Additionally, cellular interactions and developmental trajectories were analyzed to characterize cellular dynamics during prostate aging.ResultsThe single-cell transcriptome analysis of the mouse prostate during aging revealed heterogeneity across various cell types and their changes during the aging process. We found a significant increase in the proportion of mesenchymal and immune cells in aged mice. Our study unveiled alterations in genes and pathways associated with cellular senescence, oxidative stress, and regeneration in epithelial cells. Furthermore, we observed that basal cells may undergo epithelial-mesenchymal transition (EMT) to become mesenchymal cells, particularly prominent in aged mice. Additionally, immune cells, notably macrophages and T cells, exhibited a heightened inflammatory response in aged mice.ConclusionIn summary, our study provides a comparative analysis of the single-cell transcriptome of the aged and young mice prostates, elucidating cellular and molecular changes between the aged and young mice prostates.

Dietary diversity contributes to delay biological aging

AimsAs aging is a major risk factor for chronic diseases, strategies to promote healthy aging are essential. Dietary diversity has been reported to be beneficial for human health, however, the role in the biological aging process remains underexplored. Our aim was to analyse the potential link between diet diversity and aging.MethodsTwenty-two thousand six hundred participants from the National Health and Nutrition Examination Survey (NHANES) were included in this study. Dietary diversity was assessed by the dietary diversity score (DDS), which aggregated data on participants’ self-reported dietary categories for the 5 major food groups (18 subgroups) over 2 rounds. Biological age was determined using the phenotypic age, with the residual between biological age and chronological age, phenotypic age acceleration, representing biological aging advance. Weighted multivariate regressions analysis were used to examine the relationship between DDS and phenotypic age acceleration. Sensitivity, subgroup interaction and mediation analyses were employed for further analysis.ResultsHigher DDS was consistently associated with slower phenotypic age acceleration (β < 0, p < 0.001). Subgroup analyses revealed that the inverse relationship persisted across categories, with minimal interaction effects. Sensitivity analyses confirmed the robustness of results. The oxidative stress indicator glutamyltransferase partially mediated the relationship between DDS and aging [4.9% (3.6, 6.0%), p < 0.001].ConclusionDietary diversity is associated with a slower rate of biological aging, which may be due in part to reduced oxidative stress. These findings underscore the potential of a rich, broad-spectrum diet to promote healthy aging and reduce the burden of age-related diseases.

Quasi-static punch shear behavior of glass/epoxy composite: Experimental and numerical study in artificial seawater environment

Enhancing the understanding of how fiber-reinforced polymer composites respond to high-speed impacts is crucial, particularly in comparison to Quasi-Static Punch Shear Test (QS-PST). While researchers have extensively investigated QS-PST in FRP composites through experimental and numerical approaches, there's a notable gap in studies addressing the aging effects through both experimental and numerical methods. In this study, the QS-PST was conducted on S2 glass fiber reinforced epoxy composite materials aged in an artificial seawater environment. Composite plates were fabricated using Vacuum-assisted resin transfer molding (VARTM). Test samples were subjected to aging for durations of 4, 8, and 12 months. Experimental QS-PST were performed on the samples, followed by Finite Element Analysis (FEA) using LS-DYNA and the MAT 162 material model. The mechanical properties of the composite material were incorporated into the FEA and aging effects were simulated with a maximum error of 8.08% by using the proposed material model. The results indicated that the aging process led to a reduction in the punch shear strength of the composite by up to 26.84%. These findings provide valuable insights into the degradation mechanisms of composite materials in marine environments, aiding in the development of strategies for enhanced durability and performance in such conditions.

The Relationship Between the Percentage of Skeletal Muscle (SMP) and the Emergence of Degenerative Diseases (Hypertension) in Posyandu for the Elderly Mugi Sehat

Degenerative disease is a condition of continuous decline in body function with increasing age. Hypertension is a degenerative disease that is often suffered by the elderly. The aging process results in changes in body composition, often without being accompanied by weight changes. Increasing age in the elderly will experience functional limitations caused by changes in body composition, one of which is the Percentage of Skeletal Muscle. The purpose of this study was to determine the relationship between the percentage of skeletal muscle and the emergence of degenerative diseases (hypertension) in the elderly at the Mugi Healthy Elderly Posyandu. The instruments in this study used a Digital Sphygmomanometer to measure blood pressure and an Omron Karada Scan Body Composition Monitor Model HBF-375 to measure Skeletal Muscle Percentage. This research method uses a cross-sectional research design. The sample in this study is 58 people. The research was conducted at the Mugi Sehat Elderly Posyandu in the working area of the 2022 UPTD Kembar I health center in May-June 2023. The sampling technique in this study uses total sampling and data analysis using the Spearman Rank test. The results of this study from the bivariate analysis showed that there was no relationship between the Percentage of Skeletal Muscle and the emergence of degenerative diseases (hypertension) in the elderly at the Mugi Healthy Elderly Posyandu, namely the Percentage of Skeletal Muscle with hypertension P value = 0.576 (CC=-0.075).

Prevalence and Predictors of Premature Graying of Hair Before the Age of 30: A Cross-Sectional Study in SaudiArabia.

Graying is an inherent and unavoidable consequence of the aging process, impacting individuals of all genders. There are limited studies in Saudi Arabia that have examined the prevalence and predictors of premature graying of hair (PGH). This study aims to explore the prevalence and predictors of PGH before the age of 30 among the population of Saudi Arabia. This is a cross-sectional online survey that was conducted between July 2023 and February 2024 in Saudi Arabia. Binary logistic regression analysis was used to identify risk factors of having gray hair before the age of 30. A total of 1193 participants were involved in this study. A significant portion of respondents reported having gray hair before the age of 30 (55.9%). The younger population (younger than 44 years), smokers, and those who have comorbidities, have anxiety, have depression, have a family history of gray hair before the age of 30 years, have a dry scalp, suffer from vitamin or mineral deficiencies, have hair loss due to immune diseases (such as alopecia), and use minoxidil or rosemary for hair loss were more likely to have gray hair before the age of 30 years (p < 0.05). This study highlighted the high prevalence rate and associated predictors of PGH in Saudi Arabia. Identified predictors include genetic, health, and lifestyle factors. Healthcare professionals and decision makers are advised to promote the awareness of the general public on its risk factors to enhance the prevention of PGH. Public health initiatives include campaigns on smoking cessation, healthy nutrition, and mental health.

Rheology and aging of amine functionalized polyolefins

The time dependent rheo-mechanical properties of a class of associating polymers (amine-functionalized polyolefins) are investigated using rheology, differential scanning calorimetry (DSC), infrared microscopy, and small-angle x-ray scattering (SAXS) measurement. The modulus of the sample increases with time and temperature as determined by shear rheology. With higher temperature and longer equilibration time, there is a gradual decrease in the power-law scaling of storage and loss moduli in the terminal flow region and the emergence of an additional low-frequency plateau in the storage modulus. The aging behavior at different temperatures is found to be correlated with the horizontal shift factors obtained from the time-temperature superposition. With increasing aging time, there is an increase in the glass transition temperatures (DSC), and a continuous red shift in the associated amine stretching peak (Fourier-transform infrared). SAXS also shows the emergence of a dominant microstructure after aging of the sample for a long time. Based on the characterization results, an underlying microscopic origin of the aging process is proposed.

New Refined Experimental Analysis of Fungal Growth in Degraded Bio-Based Materials

When exposed to different building environmental conditions, bio-composite materials, such as hemp mortars, represent a risk of mold proliferation. This later plays a critical role in the biodeterioration of the materials when their physical properties are locally modified by the natural aging process. The primary objectives of the present work are first to assess the evolution of the surface of contaminated mortar; second, to investigate an accurate DNA extraction method that could be used for both bio-composite mortars and their fiber sources collected in situ; then, to understand the process of the proliferation of mold strains on both hemp shives and hemp mortar; and finally, to compare mold strains present in these phases to show their relationship to mold contamination and their impact on human health. In situ hemp mortar contamination behavior was investigated in the region of Pau (France) two months after hemp mortar application in extreme conditions (high humidity, low temperature, no aeration), which did not match the standard conditions under which hemp mortar must be used. The SEM observations and FTIR and pH analyses highlighted the decrease in pH level and the presence of organic matter on the mortar surface. DNA sequencing results showed that hemp shives were the main source of fungal contamination of hemp mortar. A mold population analysis showed that the most dominant phylum was Ophistokonta, which represented 83.6% in hemp shives and 99.97% in hemp mortar. The Acrostalagmus genus representatives were the most abundant, with 42% in hemp shives and 96% in hemp mortar. The interconnection between the mold strain characteristics (particularly the ability to grow in extreme environments) and the presence of hemp mortar was emphasized.

Decoding Brain Development and Aging

Abstract The aging process induces a variety of changes in the brain detectable by magnetic resonance imaging (MRI). These changes include alterations in brain volume, fluid-attenuated inversion recovery (FLAIR) white matter hyperintense lesions, and variations in tissue properties such as relaxivity, myelin, iron content, neurite density, and other microstructures. Each MRI technique offers unique insights into the structural and compositional changes occurring in the brain due to normal aging or neurodegenerative diseases. Age-related brain volume changes encompass a decrease in gray matter and an increase in ventricular volume, associated with cognitive decline. White matter hyperintensities, detected by FLAIR, are common and linked to cognitive impairments and increased risk of stroke and dementia. Tissue relaxometry reveals age-related changes in relaxivity, aiding the distinction between normal aging and pathological conditions. Myelin content, measurable by MRI, changes with age and is associated with cognitive and motor function alterations. Iron accumulation, detected by susceptibility-sensitive MRI, increases in certain brain regions with age, potentially contributing to neurodegenerative processes. Diffusion MRI provides detailed insights into microstructural changes such as neurite density and orientation. Neurofluid imaging, using techniques like gadolinium-based contrast agents and diffusion MRI, reveals age-related changes in cerebrospinal and interstitial fluid dynamics, crucial for brain health and waste clearance. This review offers a comprehensive overview of age-related brain changes revealed by various MRI techniques. Understanding these changes helps differentiate between normal aging and pathological conditions, aiding the development of interventions to mitigate age-related cognitive decline and other symptoms. Recent advances in machine learning and artificial intelligence have enabled novel methods for estimating brain age, offering also potential biomarkers for neurological and psychiatric disorders.

Brown adipose tissue: a potential target for aging interventions and healthy longevity.

Brown Adipose Tissue (BAT) is a type of fat tissue that can generate heat and plays an important role in regulating body temperature and energy metabolism. Enhancing BAT activity through medication, exercise and other means has become a potential effective method for treating metabolic disorders. Recently, there has been increasing evidence suggesting a link between BAT and aging. As humans age, the volume and activity of BAT decrease, which may contribute to the development of age-related diseases. Multiple organelles signaling pathways have been reported to be involved in the aging process associated with BAT. Therefore, we aimed to review the evidence related to the association between aging process and BAT decreasing, analyze the potential of BAT as a predictive marker for age-related diseases, and explore potential therapeutic strategies targeting BAT for aging interventions and healthy longevity.

Evaluation of the antioxidant potential for three essential oils on the properties of wood pulp paper

Purpose This study aims to determine the efficiency of three types of essential oils (EOs) as antioxidants on wood pulp paper samples: camphor oil, clove oil and lavender oil. Because of the detrimental influence of the oxidation process on wood pulp paper, this type of paper suffers significant disintegration. Design/methodology/approach The evaluation methodology is based on scanning electron microscope and three directions laser microscope, the hydrogen ion concentration measurement, color change measurement using the CIELAB system and infrared spectrum analysis, as well as investigating the mechanical properties of paper as represented by tensile and elongation to determine Young’s modulus values. Findings According to the findings, camphor was an effective antioxidant for paper samples, protecting them from the damaging effects of oxidation and the impact of artificial aging processes while also improving the mechanical properties of paper. Originality/value This paper emphasizes the concept of using environmentally friendly materials derived from EOs, owing to their ease of application, lack of environmental impact, distinctive properties, low cost and distinctive properties in both curative and preventive conservation of paper manuscripts.

Implausibility of radical life extension in humans in the twenty-first century.

Over the course of the twentieth century, human life expectancy at birth rose in high-income nations by approximately 30 years, largely driven by advances in public health and medicine. Mortality reduction was observed initially at an early age and continued into middle and older ages. However, it was unclear whether this phenomenon and the resulting accelerated rise in life expectancy would continue into the twenty-first century. Here using demographic survivorship metrics from national vital statistics in the eight countries with the longest-lived populations (Australia, France, Italy, Japan, South Korea, Spain, Sweden and Switzerland) and in Hong Kong and the United States from 1990 to 2019, we explored recent trends in death rates and life expectancy. We found that, since 1990, improvements overall in life expectancy have decelerated. Our analysis also revealed that resistance to improvements in life expectancy increased while lifespan inequality declined and mortality compression occurred. Our analysis suggests that survival to age 100 years is unlikely to exceed 15% for females and 5% for males, altogether suggesting that, unless the processes of biological aging can be markedly slowed, radical human life extension is implausible in this century.

Bitter peptide prediction using graph neural networks

Bitter taste is an unpleasant taste modality that affects food consumption. Bitter peptides are generated during enzymatic processes that produce functional, bioactive protein hydrolysates or during the aging process of fermented products such as cheese, soybean protein, and wine. Understanding the underlying peptide sequences responsible for bitter taste can pave the way for more efficient identification of these peptides. This paper presents BitterPep-GCN, a feature-agnostic graph convolution network for bitter peptide prediction. The graph-based model learns the embedding of amino acids in the bitter peptide sequences and uses mixed pooling for bitter classification. BitterPep-GCN was benchmarked using BTP640, a publicly available bitter peptide dataset. The latent peptide embeddings generated by the trained model were used to analyze the activity of sequence motifs responsible for the bitter taste of the peptides. Particularly, we calculated the activity for individual amino acids and dipeptide, tripeptide, and tetrapeptide sequence motifs present in the peptides. Our analyses pinpoint specific amino acids, such as F, G, P, and R, as well as sequence motifs, notably tripeptide and tetrapeptide motifs containing FF, as key bitter signatures in peptides. This work not only provides a new predictor of bitter taste for a more efficient identification of bitter peptides in various food products but also gives a hint into the molecular basis of bitterness.Scientific ContributionOur work provides the first application of Graph Neural Networks for the prediction of peptide bitter taste. The best-developed model, BitterPep-GCN, learns the embedding of amino acids in the bitter peptide sequences and uses mixed pooling for bitter classification. The embeddings were used to analyze the sequence motifs responsible for the bitter taste.

The evaluation of the aging process in 15-5 PH materials in terms of formability and microstructure

In this study, the effects of aging heat treatment of 15-5 PH stainless steel material on the mechanical properties, microstructure, and formability were investigated. Instead of cooling in air, cooling in the oven spontaneously was applied in the oven for the first time. Experimental studies show that aging heat treatment was applied for H900, H1025, and H1150 conditions at temperatures of 482 °C, 552 °C, and 621 °C, respectively, and 60 min for H900 and 240 min for H1025 and H1150. It has been observed that controlled cooling in the oven increases the mechanical values of the material obtained by cooling in the air by 8%–10%. The microstructures of the conditions after heat treatment were also examined, and it was observed that the cooling aging heat treatment in the oven caused precipitation hardening in the microstructure of the material. In addition, when evaluating the formability of the results using V-bending, it was observed that materials which could not withstand bending beyond 75° in Condition A (Con A), the initial state of the material, were smoothly shaped in all conditions and at all angles as a result of the cooling process in the oven. A decrease in spring-back angles has been detected due to the decrease in mechanical properties caused by increasing aging temperature. The highest spring-back value was also observed on a 75° bending die with 10.948° in the H900 condition which has the highest mechanical properties.

DEMOGRAPHIC AGING TRENDS IN EU COUNTRIES AND UKRAINE

The article provides an analysis of trends in the development of the demographic aging process in the countries of the European Union and Ukraine based on the calculation of values of demographic aging (aging coefficient, aging index, demographic burden due to the elderly, age of values aging). The obtained data allow us to draw a conclusion about the convergence of most values of population aging in the EU as a whole, as well as to identify several groups of countries with the same aging values and demographic trends. As a result of the study, the authors identified 5 groups of countries that demonstrate: 1) the highest of aging and population aging rates; 2) the average values of the index and the aging coefficient, however, show high rates of growth of the aging population and demographic burden; 3) average values of aging, but reduced growth rates of the aging population; 4) average values of aging; 5) the lowest rates and values of population aging. The authors forecast changes in the age structure of the population in the EU until 2100, which showed that in the future the share of people aged 65+ will only increase, and this will require certain changes in the social life and economy of the countries of the studied region. The demographic situation that has developed in the studied region requires constant monitoring and accounting in strategic planning at all levels, as well as improvement of the social policy of the EU countries regarding the elderly.

Survey of Knowledge, Attitudes, and Levels of Confidence Regarding Age-Related Hyperkyphosis and Its Management among Thai Physiotherapists

Background: Thorough knowledge of the management of age-related hyperkyphosis is crucial to physiotherapists’ effective handling of complex spinal deformities. Objective: This cross-sectional study investigated the knowledge, attitudes, and confidence of Thai physiotherapists regarding age-related hyperkyphosis. Methods: An online questionnaire with demographic, work-related, knowledge, attitude, and confidence questions was employed. The data analysis involved descriptive statistics, chi-square tests, and independent t-tests. Results: Complete responses were obtained from 314 physiotherapists. The correct responses amounted to 68.79% for the definition, 90.00% for causes, 14.97% for age of onset, 22.38% for prevalence, and 64.65% for the gold-standard diagnosis of hyperkyphosis. Most of the diagnostic methods involved visual examination. The respondents reported that hyperkyphosis disrupts respiratory function. The aim of treatment, according to 88.85%, was to increase spine mobility. Corrective posture exercises were the most common treatment strategy, but the range of treatments was diverse. Many cited undergraduate education as their primary evidence source. Respondents had conflicting attitudes towards “age-related hyperkyphosis is a normal aging process” but mostly positive attitudes towards the need for physiotherapy treatment. Approximately 22% reported fair confidence in treating hyperkyphosis. Conclusions: This study highlights the importance of raising awareness and enhancing knowledge, attitudes, and confidence among physiotherapists to improve care for older adults with hyperkyphosis.

A Review of Techniques for Ageing Detection and Monitoring on Embedded Systems

Embedded digital devices are progressively deployed in dependable or safety-critical systems. These devices undergo significant hardware ageing, particularly in harsh environments. This increases their likelihood of failure. It is crucial to understand ageing processes and to detect hardware degradation early for guaranteeing system dependability. In this survey, we review the core ageing mechanisms, and identify and categorize general working principles of ageing detection and monitoring techniques for Commercial-Off-the-Shelf (COTS) components that are prevalent in embedded systems: Field Programmable Gate Arrays (FPGAs), microcontrollers, Systems-on-Chips (SoCs), and their power supplies. From our review, we find that online techniques are more widely applied on FPGAs than on other components, and see a rising trend towards machine learning application for analysing hardware ageing. Based on the reviewed literature, we identify research opportunities and potential directions of interest in the field. With this work, we intend to facilitate future research by systematically presenting all main approaches in a concise way.

Chemical characteristics of Salix psammophila sand barriers are accelerated degradation by ultraviolet irradiation and water

The implementation of Salix psammophila sand barriers measures constitutes a crucial element in desertification control, providing a solid theoretical foundation for the future application and pretreatment of sand barriers in production practices. To address the specific damage types predominant in desert environments, we executed simulations of ultraviolet irradiation and rainfall phenomena on mechanical sand barriers in sandy areas and also inspected the variations in chemical properties during accelerated aging processes. The findings unequivocally demonstrate that: (1) The synergistic impact of ultraviolet irradiation and water accelerated the deformation and fracturing of the S.psammophila sand barriers, thereby causing a partial degradation of its chemical properties and conspicuous lignin oxidation; (2) The fissure of the sand barrier deepened, resulting in structural alterations. The existence of water expedites the degradation process of S.psammophila sand barriers under ultraviolet irradiation. (3)With respect to the binding form of C atoms, the carbon atoms at S.psammophila sand barriers were highly oxidized after 576 hours of accelerated aging. The components of C2 (C-O) and C3 (C=O) rising to 40.16% and 12.24% respectively, while the components of C1 (C-C) declined to 47.60%. The amount of hydroxyl (O-C-O), carbonyl (C=O), and carboxyl (O-C=O) groups increases in line with the expansion of the contact area between the sand barrier structure and ultraviolet irradiation as well as water. More free radical substances are generated, thereby causing the chemical binding properties to tend to be more stable. In summary, Ultraviolet irradiation and water change are the primary factors influencing the degradation of S.psammophila sand barriers material structure and properties. In future desertification control, it is imperative to focus on enhancing the longevity of sand barriers by ensuring their waterproofing capabilities and resistance to ultraviolet irradiation.

Senescent cell transplantation into the skin induces age-related peripheral dysfunction and cognitive decline.

Cellular senescence is an established cause of cell and tissue aging. Senescent cells have been shown to increase in multiple organs during aging, including the skin. Here we hypothesized that senescent cells residing in the skin can spread senescence to distant organs, thereby accelerating systemic aging processes. To explore this hypothesis, we initially observed an increase in several markers of senescence in the skin of aging mice. Subsequently, we conducted experiments wherein senescent fibroblasts were transplanted into the dermis of young mice and assessed various age-associated parameters. Our findings reveal that the presence of senescent cells in the dermal layer of young mice leads to increased senescence in both proximal and distal host tissues, alongside increased frailty, and impaired musculoskeletal function. Additionally, there was a significant decline in cognitive function, concomitant with increased expression of senescence-associated markers within the hippocampus brain area. These results support the concept that the accumulation of senescent cells in the skin can exert remote effects on other organs including the brain, potentially explaining links between skin and brain disorders and diseases and, contributing to physical and cognitive decline associated with aging.

Transcript errors generate amyloid-like proteins in huwman cells

Aging is characterized by the accumulation of proteins that display amyloid-like behavior. However, the molecular mechanisms by which these proteins arise remain unclear. Here, we demonstrate that amyloid-like proteins are produced in a variety of human cell types, including stem cells, brain organoids and fully differentiated neurons by mistakes that occur in messenger RNA molecules. Some of these mistakes generate mutant proteins already known to cause disease, while others generate proteins that have not been observed before. Moreover, we show that these mistakes increase when cells are exposed to DNA damage, a major hallmark of human aging. When taken together, these experiments suggest a mechanistic link between the normal aging process and age-related diseases.