Aging-induced Changes Research Articles

Age-related changes in implicit emotion processing

ABSTRACTThe aim of the present study was to compare the performance of older and younger adults on an emotional priming task. Moreover, a nonemotional priming paradigm was used as a control task to disentangle age-related changes due to emotional processing difficulties from modifications due to a decline of executive inhibitory control abilities. Twenty-two young and 22 older healthy participants completed an emotional priming task and a nonemotional priming task. In the older adult group, reaction times in the emotional priming paradigm were not influenced by any of the prime conditions, whereas both groups exhibited similar facilitation effects induced by the congruent prime in the nonemotional task. The selective lack of an emotional priming effect in older adults shown in this preliminary report suggests that aging-induced changes in the ability to recognize emotions implicitly are directly dependent on emotional processing difficulties and not due to a general age-related modification of cognitive processes.

Role of Physical Activity on Calorie-Induced Changes in Cognition and Depression of Young and Aged Humans

Normal aging is known to encounter stress. Association of aging with declining cognition and elevated depression is a phenomenon which can be modulated by lifestyle. Caloric restriction down regulates normal aging by lowering stress and has been found to protect aging processes. The present study assessed the effect of Calorie Restriction (CR) and Physical Activity (PA) on aging-induced changes on cognition and depression. Healthy male aged (20-35 years; N=312) and (55-70 years; N=392) were considered from low income and middle education population. Data related to socioeconomic status, health status, cognitive performance by mini-cog and 6CIT, depression by CES-D Scale, PA Level (PAL) and calorie intake were collected through questionnaires. AP (at-par) diet (amount consumed equivalent to the amount of energy required for 24 h) reduced cognitive function of the aged group compared to the young of the same calorie group without affecting their depression under similar conditions. CR diet improved cognition with a lowering of depression in the young subjects compared to aged of the same group. High PAL in comparison to low PAL irrespective of age and calorie intake positively modulated cognition and depression of the subjects. These results suggest that (a) young population with CR diet and aged population with AP diet are benefitted in both cognitive ability and depression (b) high PAL unlike low PAL improved both cognition and depression of both young and aged population irrespective of their calorie intake.

Effects of high temperature aging on the microstructural evolution and mechanical behavior of SAC305 solder joints using synchrotron X-ray microdiffraction and nanoindentation

Isothermal aging causes degradation in the mechanical behavior of lead-free solder materials used in microelectronic packaging, leading to significant reductions in product reliability. In this work, we have characterized the aging-induced changes that occur in the mechanical properties and creep behavior of actual solder joints. These changes occur due to microstructure evolution and residual strains/stresses in the solder material, and measurement of these evolutions are critical to developing a fundamental understanding of solder aging phenomena. The enhanced high-energy X-ray microdiffraction beamline present at the synchrotron at the Advanced Light Source at Lawrence Berkeley National Laboratory was employed to characterize the solder joints after various aging times at 125 °C. Aging-induced changes in the Sn grain orientation, Sn grain distribution, and residual strain distribution were measured. The observed microstructural evolution was then correlated with changes in the mechanical response of the solder joints measured using nanoindentation.

P49/STRAP, a Serum Response Factor Binding Protein (SRFBP1), Is Involved in the Redistribution of Cytoskeletal F-Actin Proteins during Glucose Deprivation.

The functional decline that usually accompanies adult aging also encompasses cellular changes including cytoplasmic architecture. In addition to their role in cytoskeletal structure, actin microfilaments have important roles in various cellular processes, including cell-to-cell communication and intracellular signaling. Age-related diseases and late-stage cellular morphological appearances often correlate with altered f-actin structure, which has been observed most notably in cancer. What remains less clear are the molecular pathways that may be involved in normal and premature aging-induced f-actin changes. We report herein that p49/STRAP, a serum response factor binding protein (SRFBP1), is increased with normal aging and appears to be sensitive to low glucose-exposure. Our study results suggest that increased levels of p49/STRAP expression tend to correlate with f-actin redistribution genes, particularly cofilin, while siRNA-mediated knockdown of p49/STRAP resulted in a reduction of thymosin-β4. Furthermore, with the redistribution of f-actin, we observed an increase in the intermediate filament vimentin, compatible with the notion that vimentin may be increased due to its greater role in cytoskeletal dynamics during advancing population doubling levels (PDLs) and in response to a low-glucose exposure. Taken together, these data suggest that p49/STRAP may play a role in glucose-deprivation associated cytoskeletal changes.

Differential menopause- versus aging-induced changes in oxidative stress and circadian rhythm gene markers

Menopause is characterized by the depletion of estrogen that has been proposed to cause oxidative stress. Circadian rhythm is an internal biological clock that controls physiological processes. It was analyzed the gene expression in peripheral blood mononuclear cells and the lipids and glucose levels in plasma of a subgroup of 17 pre-menopausal women, 19 men age-matched as control group for the pre-menopausal women, 20 post-menopausal women and 20 men age-matched as control group for the post-menopausal women; all groups were matched by body mass index. Our study showed a decrease in the expression of the oxidative stress-related gene GPX1, and an increase in the expression of SOD1 as consequence of menopause. In addition, we found that the circadian rhythm-related gene PER2 decreased as consequence of menopause. On the other hand, we observed a decrease in the expression of the oxidative stress-related gene GPX4 and an increase in the expression of CAT as a consequence of aging, independently of menopause. Our results suggest that the menopause-induced oxidative stress parallels a disruption in the circadian clock in women, and part of the differences in oxidative stress observed between pre- and post-menopausal women was due to aging, independent of menopause.Clinical Trials.gov.Identifier: NCT00924937

Aging Selectively Modulates Vitamin C Transporter Expression Patterns in the Kidney

In the kidney, vitamin C is reabsorbed from the glomerular ultrafiltrate by sodium-vitamin C cotransporter isoform 1 (SVCT1) located in the brush border membrane of the proximal tubules. Although we know that vitamin C levels decrease with age, the adaptive physiological mechanisms used by the kidney for vitamin C reabsorption during aging remain unknown. In this study, we used an animal model of accelerated senescence (SAMP8 mice) to define the morphological alterations and aging-induced changes in the expression of vitamin C transporters in renal tissue. Aging induced significant morphological changes, such as periglomerular lymphocytic infiltrate and glomerular congestion, in the kidneys of SAMP8 mice, although no increase in collagen deposits was observed using 2-photon microscopy analysis and second harmonic generation. The most characteristic histological alteration was the dilation of intracellular spaces in the basolateral region of proximal tubule epithelial cells. Furthermore, a combination of laser microdissection, qRT-PCR, and immunohistochemical analyses allowed us to determine that SVCT1 expression specifically increased in the proximal tubules from the outer strip of the outer medulla (segment S3) and cortex (segment S2) during aging and that these tubules also express GLUT1. We conclude that aging modulates vitamin C transporter expression and that renal over-expression of SVCT1 enhances vitamin C reabsorption in aged animals that may synthesize less vitamin C. J. Cell. Physiol. 232: 2418-2426, 2017. © 2016 Wiley Periodicals, Inc.

Quercetin delays postovulatory aging of mouse oocytes by regulating SIRT expression and MPF activity.

If no fertilization occurs at an appropriate time after ovulation, oocyte quality deteriorates rapidly as a process called postovulatory aging. Because the postovulatory aging of oocytes has detrimental effects on embryo development and offspring, many efforts have been made to prevent oocyte aging. Here we showed that quercetin prevented the decline in oocyte quality during postovulatory aging of oocytes. Quercetin treatment reduced aging-induced morphological changes and reactive oxygen species accumulation. Moreover, quercetin attenuated the aging-associated abnormalities in spindle organization and mitochondrial distribution, preventing decrease of SIRT expression and histone methylation. Quercetin also ameliorated the decrease in maturation-promoting factor activity and the onset of apoptosis during postovulatory aging. Furthermore, quercetin treatment during postovulatory aging improves early embryo development. Our results demonstrate that quercetin relieves deterioration in oocyte quality and improves subsequent embryo development.

Impact of Dietary Interventions on Noncoding RNA Networks and mRNAs Encoding Chromatin-Related Factors

Dietary interventions dramatically affect metabolic disease and lifespan in various aging models. Here, we profiled liver microRNA (miRNA), coding, and long non-coding RNA (lncRNA) expression by high-throughput deep sequencing in mice across multiple energy intake and expenditure interventions. Strikingly, three dietary intervention network design patterns were uncovered: (1) lifespan-extending interventions largely repressed the expression of miRNAs, lncRNAs, and transposable elements; (2) protein-coding mRNAs with expression positively correlated with long lifespan are highly targeted by miRNAs; and (3) miRNA-targeting interactions mainly target chromatin-related functions. We experimentally validated miR-34a, miR-107, and miR-212-3p targeting of the chromatin remodeler Chd1 and further demonstrate that Chd1 knockdown mimics high-fat diet and aging-induced gene expression changes and activation of transposons. Our findingsdemonstrate lifespan-extending diets repress miRNA-chromatin remodeler interactions and safeguard against deregulated transcription induced by aging and lifespan shortening diets, events linked by microRNA, chromatin, and ncRNA crosstalk.

What is important for aging-induced arterial stiffening, autonomic dysfunction, vascular characteristics or both?

Aging decreases arterial distensibility via changes in arterial impedance, arterial pressure, and flow wave contours; these aging-induced pathological changes in large arterial walls are qualitatively similar to those observed in hypertensive patients.1, 2, 3, 4 Therefore, hypertension is often considered as an accelerated form of aging.5

Aging-Induced Changes in Sarcoplasmic Proteome of 3 Beef Hindquarter Muscles with Differential Color Stability

Aging-Induced Changes in Sarcoplasmic Proteome of 3 Beef Hindquarter Muscles with Differential Color Stability

Mechanobiology of bone remodeling and fracture healing in the aged organism

Bone can adapt to changing load demands by mechanically regulated bone remodeling. Osteocytes, osteoblasts, and mesenchymal stem cells are mechanosensitive and respond to mechanical signals through the activation of specific molecular signaling pathways. The process of bone regeneration after fracture is similarly and highly regulated by the biomechanical environment at the fracture site. Depending on the tissue strains, mesenchymal cells differentiate into fibroblasts, chondrocytes, or osteoblasts, determining the course and the success of healing. In the aged organism, mechanotransduction in both intact and fractured bones may be altered due to changed hormone levels and expression of growth factors and other signaling molecules. It is proposed that altered mechanotransduction may contribute to disturbed healing in aged patients. This review explains the basic principles of mechanotransduction in the bone and the fracture callus and summarizes the current knowledge on aging-induced changes in mechanobiology. Furthermore, the methods for external biomechanical stimulation of intact and fractured bones are discussed with respect to a possible application in the elderly patient.

Degradation of oxygen reduction reaction kinetics in porous La 0.6 Sr 0.4 Co 0.2 Fe 0.8 O 3-δ cathodes due to aging-induced changes in surface chemistry

Abstract The modification of surface composition after long-term operation is one of the most reported degradation mechanisms of (La,Sr)(Co,Fe)O3-δ (LSCFO) cathodes for Solid Oxide Fuel Cells (SOFCs). Nevertheless, its effect on the oxygen reduction reaction kinetics of porous LSCFO cathodes has not been yet reliably established. In this work, La- and Sr-enrichment at the LSCFO surface of porous cathodes has been induced after 50 h aging at 800 °C under air. Such cation redistribution can extend up to ∼400 nm depth under the LSCFO surface as detected by high resolution Scanning Transmission Electron Microscopy-Energy Dispersive Spectroscopy maps acquired inside the cathode pores. The observed surface chemical changes hamper the oxygen surface exchange reaction at the LSCFO/gas interface. Accordingly, a suitable Electrochemical Impedance Spectroscopy analysis revealed that the oxygen ion conductivity remains practically unaltered during the aging treatment while the oxygen surface exchange resistance increases up to 1.8 times. As a result, the cathode impedance response deteriorates within the 10–0.1 Hz frequency range during the aging treatment, resulting in a total cathode area specific resistance increase of 150%. The methodology adopted has demonstrated to be very valuable for studying the degradation of SOFC cathodes produced by the modification of surface composition.

Structural response of phyllomanganates to wet aging and aqueous Mn(II)

Naturally occurring Mn(IV/III) oxides are often formed through microbial Mn(II) oxidation, resulting in reactive phyllomanganates with varying Mn(IV), Mn(III), and vacancy contents. Residual aqueous Mn(II) may adsorb in the interlayer of phyllomanganates above vacancies in their octahedral sheets. The potential for interlayer Mn(II)-layer Mn(IV) comproportionation reactions and subsequent formation of structural Mn(III) suggests that aqueous Mn(II) may cause phyllomanganate structural changes that alters mineral reactivity or trace metal scavenging. Here we examine the effects of aging phyllomanganates with varying initial vacancy and Mn(III) content in the presence and absence of dissolved Mn(II) at pH 4 and 7. Three phyllomanganates were studied: two exhibiting turbostratic layer stacking (δ-MnO2 with high vacancy content and hexagonal birnessite with both vacancies and Mn(III) substitutions) and one with rotationally ordered layer stacking (triclinic birnessite containing predominantly Mn(III) substitutions). Structural analyses suggest that during aging at pH 4, Mn(II) adsorbs above vacancies and promotes the formation of phyllomanganates with rotationally ordered sheets and mixed symmetries arranged into supercells, while structural Mn(III) undergoes disproportionation. These structural changes at pH 4 correlate with reduced Mn(II) uptake onto triclinic and hexagonal birnessite after 25days relative to 48h of reaction, indicating that phyllomanganate reactivity decreases upon aging with Mn(II), or that recrystallization processes involving Mn(II) uptake occur over 25days. At pH 7, Mn(II) adsorbs and causes limited structural effects, primarily increasing sheet stacking in δ-MnO2. These results show that aging-induced structural changes in phyllomanganates are affected by aqueous Mn(II), pH, and initial solid-phase Mn(III) content. Such restructuring likely alters manganese oxide reactions with other constituents in environmental and geologic systems, particularly trace metals and redox-active compounds.

Aging-Induced Electrical Resistance Changes in an RDX-Loaded Nitrate Ester Propellant with Polyglycol Adipate (PGA) and Polyethylene Glycol (PEG) Cross-Linked Binders Subject to Various Thermal and Moisture Environmental Conditions

ABSTRACTTwo Cyclotrimethylenetrinitramine (RDX)-loaded nitrate ester propellant formulations with polyglycol adipate and polyethylene glycol binders, respectively, are subjected to accelerated aging at 70°C in various humidity environments ranging from 0 to 100% relative humidity (RH). The electrical resistance of each sample is monitored throughout aging, along with high-performance liquid chromatography (HPLC) and gas chromatography (GC) used to measure the mass fraction of the stabilizers and nitroso/nitro derivatives at the beginning and end of aging. Stabilizer and nitroso/nitro derivative dimethyl sulfoxide (DMSO) solutions are used to evaluate the electrical resistance of these species as a function of mass fraction. The results indicate a weak correlation of the sample electrical resistance with stabilizer and nitroso/nitro mass fraction and a strong correlation with the moisture content of the sample. Moisture is postulated to affect the electrical resistance of the samples predominately by acid induced hydrolytic decomposition of the nitrate esters present and then further the cross-linked polymeric binders, causing an elevation of the mass fraction of acid species and a decrease in the sample pH.

Electrochemical battery model and its parameter estimator for use in a battery management system of plug-in hybrid electric vehicles

This paper reports the development of a battery model and its parameter estimator that are readily applicable to automotive battery management systems (BMSs). Due to the parameter estimator, the battery model can maintain reliability over the wider and longer use of the battery. To this end, the electrochemical model is used, which can reflect the aging-induced physicochemical changes in the battery to the aging-relevant parameters within the model. To update the effective kinetic and transport parameters using a computationally light BMS, the parameter estimator is built based on a covariance matrix adaptation evolution strategy (CMA-ES) that can function without the need for complex Jacobian matrix calculations. The existing CMA-ES implementation is modified primarily by region-based memory management such that it satisfies the memory constraints of the BMS. Among the several aging-relevant parameters, only the liquid-phase diffusivity of Li-ion is chosen to be estimated. This also facilitates integrating the parameter estimator into the BMS because a smaller number of parameter estimates yields the fewer number of iterations, thus, the greater computational efficiency of the parameter estimator. Consequently, the BMS-integrated parameter estimator enables the voltage to be predicted and the capacity retention to be estimated within 1 % error throughout the battery life-time.

Can Carnosine Prevent the Aging-Induced Changes of Blood Platelet and Brain Regional Monoamine Oxidase-A mRNA in Relation to its Activity?

It is well known that the monoamine oxidase (MAO) activity deregulates during aging along with anti-oxidant activity. Carnosine (β-Ala-l-His) is an endogenous dipeptide biomolecule, having both anti-oxidant and anti-glycating properties. The present study deals with the effect of carnosine on aging-induced changes in MAO-A mRNA expression of brain regions and blood platelets in relation to their MAO-A activity. Results showed that aging significantly and characteristically increased the brain regional MAO-A mRNA whereas, in blood platelets it was significantly reduced with an increase in blood platelet counts. Carnosine attenuated both aging-induced (i) increase in brain regional MAO-A mRNA expression and blood platelet count, (ii) decrease in blood platelet MAO-A mRNA expression and its (platelet MAO-A) activity without affecting the young rats’ brain regions and platelet. The present results thus suggest that carnosine attenuated and restored the aging-induced (a) increase of platelet count and (b) changes in brain regional and blood platelet MAO-A mRNA expression and (c) decrease in platelet MAO-A activity, towards their respective basal level that were observed in young rats.

Damp Heat Aging Behavior of a Polyamide-Based Backsheet for Photovoltaic Modules

This paper describes and evaluates accelerated aging of a titanium dioxide (TiO2) filled polyamide (PA) based backsheet film for photovoltaic (PV) modules. Damp heat exposure (85%RH, 85 °C) was carried out up to 2000 hrs. The backsheet was characterized using microscopic, spectroscopic, thermoanalytic, chromatographic, and mechanical methods. While Raman microscopy, infrared spectroscopy in attenuated total reflection mode (IR-ATR), scanning calorimetry (DSC), and thermal gravimetric analysis did not reveal aging-induced changes, significant yellowing was detected by ultraviolet visible near infrared (UV/VIS/NIR) spectroscopy. Depending on the stabilizer type (UV-absorbers, hindered amine light stabilizers (HALSs), and antioxidants), rather different consumption rates were ascertained by high-performance liquid chromatography (HPLC) and gas chromatography (GC). Although the ultimate mechanical properties decreased significantly, no full embrittlement was obtained after damp heat exposure of up to 2000 hrs. The observed physical and chemical aging mechanisms were classified as within the induction period without premature failure.

Role of DHA in aging-related changes in mouse brain synaptic plasma membrane proteome

Aging has been related to diminished cognitive function, which could be a result of ineffective synaptic function. We have previously shown that synaptic plasma membrane proteins supporting synaptic integrity and neurotransmission were downregulated in docosahexaenoic acid (DHA)–deprived brains, suggesting an important role of DHA in synaptic function. In this study, we demonstrate aging-induced synaptic proteome changes and DHA-dependent mitigation of such changes using mass spectrometry–based protein quantitation combined with western blot or messenger RNA analysis. We found significant reduction of 15 synaptic plasma membrane proteins in aging brains including fodrin-α, synaptopodin, postsynaptic density protein 95, synaptic vesicle glycoprotein 2B, synaptosomal-associated protein 25, synaptosomal-associated protein-α, N-methyl-D-aspartate receptor subunit epsilon-2 precursor, AMPA2, AP2, VGluT1, munc18-1, dynamin-1, vesicle-associated membrane protein 2, rab3A, and EAAT1, most of which are involved in synaptic transmission. Notably, the first 9 proteins were further reduced when brain DHA was depleted by diet, indicating that DHA plays an important role in sustaining these synaptic proteins downregulated during aging. Reduction of 2 of these proteins was reversed by raising the brain DHA level by supplementing aged animals with an omega-3 fatty acid sufficient diet for 2 months. The recognition memory compromised in DHA-depleted animals was also improved. Our results suggest a potential role of DHA in alleviating aging-associated cognitive decline by offsetting the loss of neurotransmission-regulating synaptic proteins involved in synaptic function.

Effect of ageing-induced changes in rice physicochemical properties on digestion behaviour following storage

The changes in rice grains structure and digestion behaviours were investigated following storage at 4 °C and 37 °C, respectively. Pasting study indicated that rice samples stored at 37 °C demonstrated a consistent increase in the time to peak viscosity of the Rapid ViscoAnalysis parameters, implying a quick ageing progress. Compared to the rice stored at 4 °C, aged rice (stored at 37 °C) showed a coarser morphology after cooking by SEM, suggesting a limited starch gelatinization. Consistently, ageing process led to a decrease in the leaching of starch molecules in cooking residual water, which further confirmed that starch granules in aged rice grain caused less hydration and swelling. The analysis of the amount of cell wall remnants showed that rice stored at 37 °C caused a significant increase in the amount of cell wall remnants along the storage at 37 °C, which might suggest that the cell wall structure of the rice grains became more lignified because of the ageing. Furthermore, the ageing process significantly reduced rice digestion kinetics both in rate and extent. Thus, it is assumed that ageing process leads to the cell walls becoming more strengthened and lignified, which makes the rice grain more organized in its structure, and subsequently reduces starch granules disruption and molecules leaching during the cooking. Therefore, this study suggests that the changes in digestion behaviours of rice are highly associated with the changes in rice physical and chemical properties occurred during storage and the ageing process might be another option for manipulating rice digestion properties.

Ageing-induced changes in the redox status of peripheral motor nerves imply an effect on redox signalling rather than oxidative damage

Ageing is associated with loss of skeletal muscle fibres, atrophy of the remaining fibres and weakness. These changes in muscle are accompanied by disruption of motor neurons and neuromuscular junctions although the direct relationship between the nerve and muscle degeneration is not understood. Oxidative changes have been implicated in the mechanisms leading to age-related loss of muscle mass and in degeneration of the central nervous system, but little is known about age-related changes in oxidation in specific peripheral nerves that supply muscles that are affected by ageing. We have therefore examined the sciatic nerve of old mice at an age when loss of tibialis anterior muscle mass and function is apparent. Sciatic nerve from old mice did not show a gross increase in oxidative damage, but electron paramagnetic resonance (EPR) studies indicated an increase in the activity of superoxide and/or peroxynitrite in the nerves of old mice at rest that was further exacerbated by electrical stimulation of the nerve to activate muscle contractions. Proteomic analyses indicated that specific redox-sensitive proteins are increased in content in the nerves of old mice that may reflect an adaptation to regulate the increased superoxide/peroxynitrite and maintain redox homoeostasis. Analysis of redox active cysteines showed some increase in reversible oxidation in specific proteins in nerves of old mice, but this was not universally seen across all redox-active cysteines. Detailed analysis of the redox-active cysteine in one protein in the nerve of old mice that is key to redox signalling (Peroxiredoxin 6, Cys 47) showed a minor increase in reversible oxidation that would be compatible with a change in its redox signalling function. In conclusion, the data presented indicate that sciatic nerve from old mice does not show a gross increase in oxidative damage similar to that seen in the TA and other muscles that it innervates. Our results indicate an adaptation to increased oxidation with minor changes in the oxidation of key cysteines that may contribute to defective redox signalling in the nerve.