Plant Age Research Articles

Influence of Climate and Plant Phenology (Plant Age and Growth Stage) Influence Rhopalosiphum Padi L. Abundance on Wheat Plants in Luxor Governorate, Egypt

Field experiments were conducted at El-Mattana Agricultural Research Station, Luxor Governorate, during two successive wheat growing seasons (2017/18 and 2018/19). This was to study the seasonal abundance of Rhopalosiphum padi (Hemiptera: Aphididae) on wheat plants (Giza 171 cultivar). We also investigated the effects of climate and plant phenology on the R. padi population density. Results indicated that R. padi infested wheat plants from December 17, 2017, until April 8, 2018, within the first growing season (2017/18), and from Jan. 27th, 2019, up to April 13th, 2019, within the second growing season (2018/19). The cumulative counts of R. padi in growing season one was 9486.17, and in growing season two, 3444.00 individuals. The mean population of R. padi per 10 tillers over the whole first season was 80.12 ± 7.90, and for the second season, 42.36 ± 2.96. The first season, December, January, and February, had the most favorable climate for R. padi population growth (measured during weekly inspections). In contrast, February and March were more favorable in the second season. R. padi was not detected on the wheat during the wheat maturation period within both growing seasons. The combined effects of climate and plant phenology strongly correlate with R. padi population density, with explained variance (EV) of 93.86% in the first season and 99.11% in the second season. Daily mean maximum temperature was the most influential variable explaining changes in total R. padi population, with EV 28.37%in the first season and 28.62% in the second season. The data provided here can assist in the design of Integrated Pest Management (IPM) programs for aphid control on wheat plants.

The Impact of Rainfall on Oil Palm Production: A Case Study in Berau Regency, East Borneo, Indonesia

This study examines the impact of rainfall on oil palm growth and productivity in the Berau region of East Borneo, Indonesia. The research utilized qualitative and quantitative field studies, employing correlation and regression analysis. The study focused on the oil palm plantation area as its subject. Data was collected from a plantation in the Berau region of East Borneo. The plantation has homogeneous plant age (same planting year, 2009) and homogeneous progeny (Scofindo variety) and is included in the company's core plantation. The observation parameters included rainfall, rainy days, fresh fruit bunches, and plant productivity. It was found that rainfall and rainy days have an impact on oil palm productivity. The regression results indicate a positive correlation between rainfall and oil palm productivity, with a correlation coefficient of 0.025109171. However, rainy days have a negative effect on oil palm productivity, with a correlation coefficient of -0.036778304, despite being positively correlated with rainfall. The correlation coefficient of 0.768386269 indicates a direct impact on reducing oil palm productivity. Rainfall indirectly affects oil palm productivity by influencing temperature, humidity, intensity, and length of daily irradiation. These factors directly affect the productivity of oil palm plants.

Efficacy of some botanical insecticides against <em>Aphis gossypii</em> Glover (Hemiptera: Aphididae) on chrysanthemum

An evaluation of various botanical insecticides to control Aphis gossypii and its impact on aphid population dynamics on chrysanthemum plants was investigated. In order to control A. gossypii on chrysanthemum, the effectiveness of several botanical insecticides extracted from Melia azedarach, Toona sinensis, and Chrysanthemum cinerariaefolium was investigated in the current study. The research was carried out in the experimental field of the Indonesian Ornamental Crops Research Institute under plastic house conditions. Five concentrations, i.e., 1.5, 2.0, 2.5, 3.0, and 3.5 g/L of three plant extracts, M. azedarach, T. sinensis, and C. cinerariaefolium, were sprayed on chrysanthemum cultivar White Fiji after 28 to 84 days after planting. A. gossypii had a dynamic population that changed according to the plant’s developmental stages. In vegetative growth, the alate adult and nymphal stages were dominant, and the population of nymphs increased along the plant ages. The insect colonized young leaf surfaces in the terminal apices. During the reproductive stages, the population of alate adults diminished, and the distribution of the insect extended to mature, old leaves, flower buds, and bloomed flowers. The application of several botanical insecticides revealed various responses of aphid populations. Among the tested insecticides, C. cinerariaefolium extract at 3.0 and 3.5 g/L demonstrated the highest average percentage efficacy (76 and 72%) and was the most consistent in suppressing the population. The results of this study indicate the potential efficacy of botanical insecticides against A. gossypii suggesting a different approach to efficient and environment-friendly chrysanthemum pest management.

Golden Barrel Cactus: Unveiling Its Potential as a Functional Food and Nutraceutical Source.

A comprehensive analysis of phytochemicals, digestive stability, and bioaccessibility was conducted on a golden barrel cactus extract from 3- and 6-year-old plants. Both ages contained lutein and four chlorophyll derivatives (chlorophyll a, b, pheophytin a, and b), but younger cacti revealed a significantly higher abundance. Total phenolics reached 3545.35 mg gallic acid equivalent/100 g dry weight in the 3-year-old extracts compared to 2557.96 mg/100 g in the older ones. Antioxidant activity, assessed by DPPH, ABTS, and FRAP assays, was consistently higher in the younger group. While digestion impacted all compounds, lutein exhibited relative stability at 69.03% and 58.33% retention for 3- and 6-year-old extracts, respectively. Chlorophylls displayed lower stability (37.64% and 33.34% remaining for younger and older cacti) despite showing higher bioaccessibility (73.385% and 64.65%). Phenolics also demonstrated promising bioaccessibility (76.39% and 69.88%) despite moderate digestive degradation (60.52% and 56.89% retained). Conclusively, all investigated attributes-phytochemical content, digestive stability, and bioaccessibility-favored the younger golden barrel cactus extracts. This highlights the crucial role of plant age in maximizing the potential health benefits of these extracts.

Features of the formation of the ontogenetic structure of the cenopopulation and seed productivity of <i>Sanguisorba officinalis</i> in the conditions of forest and forest‐steppe vegetation of the Middle Volga region, Russia

The purpose of this work is to study the features of the life cycle of Sanguisorba officinalis in various types of vegetation in the Republic of Tatarstan, Russia. We used traditional methods for studying the cenopopulation, analysis of the calendar age of plants of various ontogenetic states, and potential and actual seed productivity. Under broad‐leaved forest conditions, a young normal population was formed on the basis of slowly developing plants. In conditions of open communities, young cenopopulations were formed on the basis of intensive seed reproduction processes. The young cenopopulation can be recommended for further study in biomass exploitation. The predominance of long‐lived vegetative and immature plants in the conditions of a broad‐leaved forest is associated with difficulties in the transition of individuals to sexual reproduction. The maintenance of numbers is carried out through vegetative growth and reproduction. With deterioration of ecological and coenotic conditions, above‐ground shoots were not formed but regressive‐quasisenile coenopopulations were formed. Seed productivity was determined by ecological and cenotic conditions. On the steppe meadow of the Bugulma district, the lowest seed productivity index was 9 %. The lowest indicator of the trait “Number of full‐fledged seeds” (the average indicator was 7), low seed germination and germination energy were also noted there. The highest values of these indicators were noted n glades of the broad‐leaved areas of the same region. The realization of the reproductive system of Sanguisorba officinalis even under extreme conditions and the survival of the species in various types of vegetation suggest that the territory of the Republic of Tatarstan is included in the ceno‐area of the species. This will permit the creation of plantations in the republic.

Smart Irrigation and Pesticides System for Enhancing Harvesting in Agriculture Using IoT

Agriculture plays a vital role in the economy of many countries, serving as a primary source of food. However, traditional farming practices often face challenges such as inefficient water usage, over-reliance on pesticides, and inconsistent monitoring of crop conditions. In recent years, the Internet of Things (IoT) has emerged as a promising technology to address these challenges by providing real-time monitoring and control of agricultural processes. This leads in excessive water use, which has an impact on agricultural output. This integrated system means that farmers only need to perform little manual monitoring. The opening and closing of the reservoir and field gates, as well as the verification of the plant's age and the matching optimal water level necessary for that time of plant age, are all automated. Farmers may use their mobile phones to check the water level in the field since they are continually receiving updates on the field work. The most labour-intensive and time consuming activity in agriculture is water management. In our project, the water level in the agriculture harvest sting is measured, and if it is lower than the required level, the paddy field is automatically irrigated for the water source or tank using a water pump. It can comprehensively monitor air temperature and humidity, light intensity, ultraviolet light and other environmental elements. In specific use, the agricultural production environment monitoring system starts from the actual needs of the plantation zone and is assembled according to the environmental elements it needs to monitor.

Evaluation of Anaerobic Soil Disinfestation to Reduce Soilborne Diseases in Soilless and Soil-Based Substrates for Specialty Cut Flower Production.

Anaerobic soil disinfestation (ASD) is a nonchemical soil treatment where an easily decomposable carbon source is incorporated into soil, which is then irrigated to saturation and tarped to create anaerobic conditions, which prompts shifts in the soil microbiota from aerobes to anaerobes. ASD has been tested successfully for soilborne disease management in a variety of cropping systems but has not been sufficiently investigated in ornamentals. In this study, ASD was evaluated in soil-based and soilless substrates commonly used in specialty cut flower production using two model pathosystems: Rhizoctonia solani-Zinnia elegans and Phytophthora drechsleri-Gerbera jamesonii. Each substrate was mixed with pathogen-infested vermiculite and amended with either wheat bran, tomato pomace, or soybean meal as the carbon source. Amended substrates were incubated at 25°C for 4 weeks and used as growing substrates for the two crops mentioned above, which were monitored weekly for disease development for up to 5 weeks posttransplant. Additional experiments tested the effect of plant age and inoculum concentration in the substrate on ASD efficacy. Results showed that ASD has the potential to be deployed successfully for the control of Rhizoctonia stem rot in both substrates. Conversely, ASD was not effective at controlling Phytophthora crown rot on gerbera daisy in any of the experiments conducted in this study. More research is needed to understand the influence of carbon amendments, inoculum thresholds, and environmental conditions on ASD efficacy.

Pembelajaran Aplikasi Pengukur Kandungan Klorofil pada Daun Tanaman Padi (Oryza sativa L.)

Rice is a staple food in Indonesia, so the demand will continue to increase along with the population growth. Rice cultivation in Indonesia is known for two planting systems, namely wet rice that requires water and dry rice planted in fields that do not require special irrigation. Several regions in Indonesia have types of dry rice, including the almost extinct Fakhaito or black rice from Nias Regency, with a plant age of six months until harvest. The productivity of dry rice is very low, affecting farmers' income due to suboptimal fertilization technology. The aim of this research is to provide learning to students using the SPAD-502Plus chlorophyll meter to detect the growth and health of dry rice plants from Nias. The research results show that treatment with liquid organic fertilizer correlates positively with leaf color, where the amount of chlorophyll is higher in very green rice leaves and vice versa, the amount of chlorophyll decreases in plants with reduced green matter. The use of the SPAD-502Plus chlorophyll meter, by measuring directly, is faster, non-destructive, comfortable, and does not disturb plant growth.

Comparative Evaluation of Proximate, Mineral, Vitamins, Phytochemical and Antioxidant Properties of Pulp and Seeds of Doum Palm (Hyphaene thebaica) in India

Medicinal plants are natural gift that contains phytochemicals that are ecofriendly, effective and non-toxic. These chemicals can also prevent antimicrobial resistance. However, the concentration of phytochemicals and nutritional properties underlines certain variation due to geographical location, age of plant, storage condition, extraction methods, amongst others. Therefore, this study was carried out to compare the proximate, mineral, vitamins, phytochemical and antioxidant properties of pulp (DPML) and seeds of Doum palm (DPSM) (Hyphaene thebaica) in India. Proximate evaluation of DPML revealed that it had higher (p<0.05) levels of moisture (8.77 %), crude fibre (13.06 %), ether extracts (1.21 %), ash (8.21 %), carbohydrate (69.33 %) and lower protein (6.21 %) relative to DPSM which contained moisture, crude protein, crude fibre, ether extract, ash and carbohydrates at 6.93 %, 8.07 %, 11.69 %, 0.09 %, 6.44 % and 54.08 % respectively. DPML had higher (p<0.05) concentration of calcium (387.21 mg/100g), phosphorus (2956.1 mg/100g), potassium (175.1 mg/100g), manganese (28.03 mg/100g), magnesium (186.1 mg/100g), zinc (10.33 mg/100g), copper (8.61 mg/100g), sodium (228.1 mg/100g) and iron (9.11 mg/100g) compared to DPSM which contained calcium, phosphorus, potassium, manganese, magnesium, zinc, copper, sodium and iron at 206.8 mg/100g, 1600.2 mg/100g, 113.5 mg/100g, 15.14 mg/100g, 102.4 mg/100g, 10.05 mg/100g, 5.78 mg/100g, 190.2 mg/100g and 6.08 mg/100g respectively. Doum palm pulp meal contained greater concentrations (p<0.05) of vitamin A (9.38 iu/100g), vitamin B1 (26.01 μg/100g), vitamin B1 (11.73 μg/100g), vitamin B6 (57.10 μg/100g), vitamin B12 (15.61 μg/100g), vitamin C (122.6 μg/100g) and vitamin E (31.62 μg/100g) relative to 5.06 iu/100g, 21.48 μg/100g, 9.45 μg/100g, 42.08 μg/100g, 10.20 μg/100g, 95.18 μg/100g and 23.49 μg/100g reported for vitamin A, vitamin B1, vitamin B6, vitamin B12, vitamin C and vitamin E respectively. Doum palm pulp meal produced higher concentrations of phenols (82.10 mg/g), flavonoids (39.15 mg/g) and saponins (2.16 mg/g) and lower concentrations of tannins (11.72 mg/g) and alkaloids (6.13 mg/g) relative to doum palm seed meal (p<0.05) which contained phenols (65.72 mg/g), flavonoids (30.86 mg/g), tannins (18.83 mg/g), alkaloids (9.17 mg/g) and saponins (1.91 mg/g). DPPH was greater (p<0.05) in doum palm pulp meal with 31.82 % compared to doum palm seed meal (26.07 %). It was concluded that DPML and DPSM is loaded with nutrients and other medicinal properties making them useful in livestock production

Plant Growth Regulators and Rooting Media: A Viable Approach for Growth and Performance of Citrus

Plant growth regulators accompanied with appropriate growth medium can boost up the growth and performance of plants to a greater extent. PGRs such as auxin, gibberellin and cytokine play an exaggerative role in growth and development of the citrus. Moreover, the study reflects a clear overview of the impact of PGRs and different growth media to encourage rooting percentage, number of leaves, survival percentage, sprouting time, and root length of citrus cuttings. Citrus is a versatile fruit crop having a huge number of varieties, cultivars, and species; comes in different colours and flavours. Although, there are different methods used in the multiplication of citrus plant which includes budding, grafting, cutting, air layering, etc. However, propagation by cutting technique is the most commercial and farmer’s friendly approach. Different elements such as concentration of PGRs, age of plants and environmental factors are responsible for the successful growth of the citrus cuttings. Naphthalene acetic acid (NAA) and indole-3-butyric acid (IBA) are the two types of auxin, which are most commonly utilized in citrus propagation. Rooting medium plays a crucial role in the supply the minerals and availability of water in citrus. An absolute rooting medium must have more enough nutrients and water holding capacity with better drainage as well as sufficient air spores for excellent gaseous exchange that fruitfully influence the rooting ability and also have a profound effect on growth and development in citrus cutting. The best time for cutting of citrus stems is in between the July and August.

In vivo regeneration efficiency of enset (Ensete ventricosum (Welw.) Cheesman) as affected by age of parent plant and corm proportion

In cultivation, E. ventricosum is propagated clonally with adventitious buds sprouting from callus formed on the cut surfaces of the corm. The present study was conducted to enhance the efficiency of this indigenous enset propagation technique by identifying the optimum age of parent plants and corm types for maximum shoot production. This study was conducted using corms in the range of 12–18, 20–28, 29–38, and 40–50 cm diameter, corresponding to four age classes (1, 2, 3 & 4 years old) and three corm treatments (whole, half and quarter) were arranged in 3 × 4 factorial combination and layout in randomized complete block design with three replications. The statistical program SAS Version 9.3 was used to examine the data. The result of the study showed that all of the parameters considered were significantly affected by the treatments or their interaction effects. The number of shoots produced per corm was significantly (p < 0.01) affected by the interaction of the age of the parent plant and corm treatment. The average number of shoots produced per corm was between 43 and 443 shoots per com. The highest shoot numbers (443 shoots per corm) were recorded on the 3-year plant using quarter corm; whereas the lowest number of shoots (43 shoots per corm) was obtained from whole corms of the 1-year plant. In general, the results of the study showed that the use of quartered corms of 3-year-old parent plants appears to be the efficient technique to regenerate enset in vivo.

The effect of grafting on vegetative and reproductive traits of tomato

Relevance. Tomato (Solanum lycopersicum L.) is one of the most important vegetables in the world. Every year the number of identified viruses and diseases increases, infection with which causes significant crop losses and significantly worsens the quality of agricultural products, especially in tomatoes. Material and methods. In the present study we compared the growth of the vegetative and reproductive traits of tomato plants ‘Dokia’, ‘TY Red 250’ and ‘Pilabi’ grated onto bacterial wilt (caused by Ralstonia solanacearum) and Fusarium wilt (Fusarium oxysporum) diseases tolerant rootstock “Spider” (Takii seed, Japan). The non-grafted (control - CT) and grafted tomato plants (GR) were transplanted on 4 March of 2021 to the four season (vinyl) greenhouse on substrate perlite (mixture of perlite no. 1 and no. 3) and cultivated until September 20. Results. The results showed that responses of the tomato cultivars to grafting combination was different, where agronomical traits depends on the each cultivar’s features can be ranged. The reduction of the values of stem diameter (SD), leaf length and width (LW), fresh fruit weight (FFW), fruit diameter (FD), fruit pericarp thickness (FPT) and fruit hardness (FH) with aging of plants and rising ambient temperature was detected. However, fruit soluble solids among all cultivars regardless of treatments were slightly increased. The index of fruit yield per truss (FYT) significantly decreased among all cultivars after 10th truss regardless of the treatments when the daily temperature increased from July to August. In grafted tomato ‘TY Red 250’ were identified the highest fruit yield per plants (FYP) than in CT plants, whereas in other tomatoes did not found similar differences between CT and GR plants.

Morphology Characteristics of Local Rice (Oryza Sativa L.) Rice Lands of Long Tebulo Village, North Kalimantan

Local rice (Oriza sativa L). is an annual plant cultivated by local people once a year. Local rice in the Malinau Regency area has different names for rice cultivars based on the local community's ethnicity or the person who brought the seeds. The aim of this research is to find out the local rice cultivars in the rice fields in Long Tebulo village and to find out the characteristics of the local rice fields in Long Tebulo village. This research was carried out from November 2020 to February 2021. The methods used in the research were qualitative analysis, exploration and characterization. The research results are available. There are 10 types of local rice cultivars, there are 6 sticky rice cultivars including Adan Saleng, Adan Bala, Adan Pute, Uro, Pulut Embeng, Pulut Bu'ung, and 4 ordinary rice cultivars, namely Bere, Bawang, and Gula/bread, based on plant characteristics. Rice has different ages. plant height, panicle length, grain shape, age, and rice color.

Assessing Field Degradation of Photovoltaic Modules by Near‐Infrared Absorption Spectroscopy of Ethylene Vinyl Acetate Encapsulant

The degradation status of polymer encapsulants in photovoltaic (PV) modules with backsheets of various compositions is evaluated by near‐infrared absorption (NIRA) spectroscopy. The method is used to identify backsheet types and to assess the content of carbonyl species in the ethylene vinyl acetate copolymer encapsulant expressed as a carbonyl index (CI). The CI distributions are collected for PV modules of different ages and in different climatic conditions from twelve PV installations in Europe (EU) and South Africa (ZA) and the peak of the index distribution is found to be related exponentially to the total dose of solar irradiation. The CI growth rate with the irradiation dose in EU is around two times larger than in ZA, most probably due to more massive moisture ingress into PV modules installed in a more humid EU climate. For similar total solar exposures, the CI is found to depend on the backsheet structure. Repeated measurements on the same plant after an additional 2 years of module operation confirm the capacity of NIRA spectroscopy to track the temporal progression of encapsulant degradation using CI as a quantifier depending on the encapsulant‐backsheet bill of materials, plant age, and climatic zone.

Differential responses of two fenugreek (Trigonella foenum-graecum L.) landraces pretreated with melatonin to prolonged drought stress and subsequent recovery

BackgroundDrought impairs growth, disturbs photosynthesis, and induces senescence in plants, which results in crop productivity reduction and ultimately jeopardizes human food security. The objective of this study was to determine major parameters associated with drought tolerance and recovery ability of fenugreek (Trigonella foenum-graecum L.), by examining differential biochemical and phenological responses and underlying enzyme activities as well as melatonin roles during drought stress and re-watering for two contrasting landraces. Moreover, the relative expression of three key genes involved in the biosynthesis pathway of diosgenin, including SQS, CAS, and BG, was investigated.ResultsDepending on the conditions, drought stress enhanced the activity of antioxidant enzymes and the osmoregulating compounds, non-enzymatic antioxidants, hydrogen peroxide content, and lipid peroxidation levels in most cases. Severe drought stress accelerated flowering time in Shushtar landrace (SHR) but had no significant effects on Varamin (VR). Pretreatment with melatonin delayed flowering time in SHR and caused high drought resistance in this landrace. Furthermore, melatonin significantly enhanced drought adaptability in VR by improving plant recovery ability.DiscussionBased on our results plants’ responses to drought stress and melatonin pretreatment were completely landrace-specific. Drought stress caused an increase in the relative expression of CAS gene and ultimately the accumulation of steroidal saponins in SHR. Melatonin compensated for the decrease in biomass production due to drought stress and finally increased steroidal saponins performance in SHR. Our study showed that melatonin can improve drought stress and recovery in fenugreek, but different factors such as genotype, melatonin concentration, and plant age should be considered.

Cell wall as a barrier for protein extraction from tomato leaves: A biochemical study

Solanum lycopersicum (Tomato) leaves and stems are considered waste. Valorization of this waste can be achieved by for example the extraction of proteins. This prospect is promising but currently not feasible, since protein extraction yields from tomato leaves are low, amongst other due to the (physical) barrier formed by the plant cell walls. However, the molecular aspects of the relationship between cell wall properties and protein extractability from tomato leaves are currently not clear and thus objective of this study. To fill this knowledge gap the biochemical composition of plant cell walls was measured and related to protein extraction yields at different plant ages, leaf positions, and across different tomato accessions, including two Solanum lycopersicum cultivars and the wildtype species S. pimpinellifolium and S. pennellii. For all genotypes, protein extraction yields from tomato leaves were the highest in young tissues, with a decreasing trend towards older plant material. This decrease of protein extraction yield was accompanied by a significant increase of arabinose and galacturonic acid content and a decrease of galactose content in the cell walls of old-vs-young tissues. This resulted in strong negative correlations between protein extraction yield and the content of arabinose and galacturonic acid in the cell wall, and a positive correlation between the content of galactose and protein extraction yield. Overall, these results point to the importance of the pectin network on protein extractability, making pectin a potential breeding target for enhancing protein extractability from tomato leaves.

Carbon stock estimation in post-mining reclamation area of open coal mining in PT Berau Coal, Berau, East Kalimantan

Postmining land reclamation and revegetation activities play an important role in restoring vegetation, degraded land and controlling climate change because CO2 is sinking from the atmosphere due to the impact of mining activities. This study aims to estimate the potential for above-ground and below-ground carbon and CO2 absorption capacity at the Binungan Mine Operations Site (BMO), PT Berau Coal, Berau, East Kalimantan. Vegetation analysis was used to estimate the potential carbon stock and CO2 absorption capacity at plant ages 1-10 years using the purposive sampling method of 5 plots (20 x 20 m) each in the post-mining area studied. Biomass and carbon stocks were estimated using existing allometric models. The results showed 14 tree species from 10 families dominated by Fabaceae. Biomass was estimated at 1.13 - 128.31 Mg ha-1, carbon stocks were 0.53-60.3 Mg ha-1, and CO2 absorption was 1.95 - 221.32 Mg ha-1. Reclamation and revegetation in 10 year-old plants on reclamaied land can restore biomass, carbon stock, and CO2 absorption capacity of 70% from the initial baseline.

Expression changes of miRNA-regulated genes associated with the formation of the leafy head in cabbage

The vegetative development of cabbage (Brassicaoleracea var. capitata) passes through seedling, rosette, folding and heading stages. Leaves that form the rosette are large and mostly flat. In the following developmental stages, the plants produce leaves that curve inward to produce the leafy head. Many microRNAs and their target genes have been described participating in leaf development and leaf curvature. The aim of this study is to investigate the role of miRNA-regulated genes in the transition from the rosette to the heading stage. We compared the miRNA and gene abundances between emerging rosette and heading leaves. To remove transcripts (miRNAs and genes) whose regulation was most likely associated with plant age rather than the change from rosette to heading stage, we utilized a non-heading collard green (B. oleracea var. acephala) morphotype as control. This resulted in 33 DEMs and 1 998 DEGs with likely roles in the transition from rosette to heading stage in cabbage. Among these 1 998 DEGs, we found enriched GO terms related to DNA-binding transcription factor activity, transcription regulator activity, iron ion binding, and photosynthesis. We predicted the target genes of these 33 DEMs and focused on the subset that was differentially expressed (1 998 DEGs) between rosette and heading stage leaves to construct miRNA-target gene interaction networks. Our main finding is a role for miR396b-5p targeting two Arabidopsis thaliana orthologues of GROWTH REGULATING FACTORs 3 (GRF3) and 4 (GRF4) in pointed cabbage head formation.

Pengaruh dosis kalium terhadap intensitas penyakit bercak daun yang disebabkan oleh Cercospora personata pada tanaman kacang tanah

Cercospora personata is a pathogen that causes leaf spot disease, as one of the important diseases of peanut plants, known to reduce the rate of photosynthesis to reduce plant production by up to 50%. The handling of Cercospora attacks so far is still focused on the method of spraying fungicides. Potassium fertilization is known to increase plant resistance from one of the Cercospora attacks. The right dosage to overcome Cercospora attacks needs to be studied so that the method of fertilizing potassium in peanut plants can be implemented effectively. This study was conducted in an experimental garden using 5 dosages of potassium and repeated 3 times. The composition of the treatment used is: D0 (control), D1 (2 gr of potash fertilizer per plant), D2 (4 gr of potash fertilizer per plant), D3 (6 gr of potash fertilizer per plant) and D4 (8 gr of potash fertilizer per plant). Weekly observations were made on the variables of plant height, number of leaves, number of saplings, as well as the intensity of disease attacks. The results showed that the dose of potassium had a significant effect on several components of plant growth and the intensity of disease attacks. The application of potassium as a fertilizer has a significant influence on the observation components of the number of leaves (plant age 8, 10, and 12 MST), the number of saplings at plant age 10 MST, and the intensity of leaf spot disease attacks (plant age 8, 10, and 12 MST). Plants that were not given potassium showed leaf spot disease attacks with the highest intensity compared to the other four treatments. This pattern was seen at observation ages of 8, 10, and 12 MST.

Seasonality and longer-term development generate temporal dynamics in the Populus microbiome.

Temporal variation in community composition is central to our understanding of the assembly and functioning of microbial communities, yet the controls over temporal dynamics for microbiomes of long-lived plants, such as trees, remain unclear. Temporal variation in tree microbiomes could arise primarily from seasonal (i.e., intra-annual) fluctuations in community composition or from longer-term changes across years as host plants age. To test these alternatives, we experimentally isolated temporal variation in plant microbiome composition using a common garden and clonally propagated plants, and we used amplicon sequencing to characterize bacterial/archaeal and fungal communities in the leaf endosphere, root endosphere, and rhizosphere of two Populus spp. over four seasons across two consecutive years. Microbial community composition differed among seasons and years (which accounted for up to 21% of the variation in microbial community composition) and was correlated with seasonal dissimilarity in climatic conditions. However, microbial community dissimilarity was also positively correlated with time, reflecting longer-term compositional shifts as host trees aged. Together, our findings demonstrate that temporal patterns in tree microbiomes arise from both seasonal fluctuations and longer-term changes, which interact to generate unique seasonal patterns each year. In addition to shedding light on two important controls over the assembly of plant microbiomes, our results also suggest future studies of tree microbiomes should account for background temporal dynamics when testing the drivers of spatial patterns in microbial community composition and temporal responses of plant microbiomes to environmental change.IMPORTANCEMicrobiomes are integral to the health of host plants, but we have a limited understanding of the factors that control how the composition of plant microbiomes changes over time. Especially little is known about the microbiome of long-lived trees, relative to annual and non-woody plants. We tested how tree microbiomes changed between seasons and years in poplar (genus Populus), which are widespread and ecologically important tree species that also serve as important biofuel feedstocks. We found the composition of bacterial, archaeal, and fungal communities differed among seasons, but these seasonal differences depended on year. This dependence was driven by longer-term changes in microbial composition as host trees developed across consecutive years. Our findings suggest that temporal variation in tree microbiomes is driven by both seasonal fluctuations and longer-term (i.e., multiyear) development.