Agastache rugosa, is a perennial medicinal plant commonly used in Chinese herbalism, and may have anti-atherogenic and antibacterial properties. Here in this study, we investigated the growth and variations in antioxidant contents of A. rugosa in response to nutrient solution and photosynthetic photon flux density (PPFD) with artificial lighting for a hydroponics culture. Fluorescent light at 150, and 200 μmol·m-2·s-1 PPFD with a 16/8 (light/dark) photoperiod, combined with four different nutrient solutions [developed by Horticulture experiment station in Japan (HES), University of Seoul (UOS), Europe vegetable research center (EVR), Otsuka-house 1A (OTS)], were used in a hydroponics culture system for 6 weeks. The shoot and root dry weights of A. rugosa grown with the OTS were significantly higher than those of other nutrient solutions. The amount of tilianin was the highest grown with the OTS, followed by EVR, HES, and UOS. Total acacetin content was the highest in A. rugosa grown under EVR which was statistically similar with OTS. The A. rugosa grown under 200 μmol·m-2·s-1 PPFD produced higher fresh weight and both acacetin and tilianin contents than that grown under 150 μmol·m-2·s-1 PPFD. The present results suggested that OTS along with 200 μmol·m-2·s-1 PPFD could be an optimum growing condition for better growth and higher accumulation of tilianin and acacetin contents in A. rugosa with hydroponic culture systems in a plant factory.