Afrotropical Research Articles

Phylogenomics and biogeography of the small carpenter bees (Apidae: Xylocopinae: Ceratina)

Small carpenter bees in the genus Ceratina are behaviourally diverse, species-rich, and cosmopolitan, with over 370 species and a range including all continents except Antarctica. Here, we present the first comprehensive phylogeny of the genus based on ultraconserved element (UCE) phylogenomic data, covering a total of 185 ingroup specimens representing 22 of the 25 current subgenera. Our results support most recognized subgenera as natural groups, but we also highlight several groups in need of taxonomic revision – particularly the nominate subgenus Ceratina sensu stricto – and several clades that likely need to be described as new subgenera. In addition to phylogeny, we explore the evolutionary history of Ceratina through divergence time estimation and biogeographic reconstruction. Our findings suggest that Ceratinini split from its sister tribe Allodapini about 72 million years ago. The common ancestor of Ceratina emerged in the Afrotropical realm approximately 42 million years ago, near the Middle Eocene Climatic Optimum. Multiple subsequent dispersal events led to the present cosmopolitan distribution of Ceratina, with the majority of transitions occurring between the Afrotropics, Indomalaya, and the Palearctic. Additional movements also led to the arrival of Ceratina in Madagascar, Australasia, and a single colonization of the Americas. Dispersal events were asymmetrical overall, with temperate regions primarily acting as destinations for migrations from tropical source regions.

Predicting & Analysing Discharge and Sediment of Sore Catchment, Baro-Akobo Sub Basin Using Saturation Excess Model, Western Ethiopia

Soil erosion, land degradation, and soil loss are major problems in Ethiopia's Baro-Akobo Basin. In the Ethiopian highlands, many hydrological models have been employed to forecast sediment and discharge in watersheds of varying sizes. PED mode was used for this study because it requires fewer data and is well recognized by academics in Ethiopia's Upper Blue Nile. The parameter efficient semi-distributed watershed model (PED) was used to study the prediction of discharge and sediment in the Sore watershed. The objective of this study was to analyze and forecast the sediment and discharge from the Sore catchment in the western Ethiopian region of the Baro-Akobo Sub basin. The selected watershed was Sore (1665.5 Km2) in the Baro-Akobo River Basin. The stream flow and sediment data for the Sore watershed from 2005 to 2013 and 2014 to 2018 were used for the model's calibration and validation by the Ministry of Water and Electricity. For the Sore watershed, the daily time step scale model efficiency of PED-W was found to be NSE= 0.40 and NSE= 0.44 for the calibration of sediment and discharge, respectively. Similarly, NSE values for the Sore watershed's NSE= -0.82 and PED-W's discharge and sediment during the validation period were found. As a result, there was a range of uneven to acceptable agreement between the simulated and observed discharge and sediment at daily time steps. In comparison to the daily time step scale, the models' performance varied and performed better on the monthly time step scale. According to the overall model performance, the PED-W model was shown to be less accurate than the SWAT model for estimating stream flow and sediment output. The PED model, on the other hand, was exactly the same as the previously employed SWAT model and was used for calibration and validation at monthly time steps.

Review of the Afrotropical members of the genus Eridachtha Meyrick, 1910 (Lepidoptera: Lecithoceridae: Lecithocerinae), with descriptions of 12 new species

The genus Eridachtha Meyrick, 1910 of the subfamily Lecithocerinae is revised for the Afrotropical region. Twelve new species are described within it: six from Kenya (Eridachtha campanella Park, sp. nov., E. cardialis Park, sp. nov., E. centriella Park, sp. nov., E. crococorypha Park, sp. nov. (which also occurs in Uganda), E. nairobiensis Park, sp. nov., and E. phiarella Park, sp. nov.), two from Tanzania (E. arumeruna Park, sp. nov. and E. kilimanjaroensis Park, sp. nov.), three from Uganda (E. pholexana Park, sp. nov., E. cupaformis Park, sp. nov., and E. atrihispida Park, sp. nov.), and one from South Africa (E. capensis Park, sp. nov.). Three of them (whose males remain unknown)—E. cupaformis, E. atrihispida and E. crococorypha—share with E. gymnopalpa (Janse, 1963), comb. nov., a yellowish-orange ground colour of the forewing, and are thus grouped in the E. gymnopalpa species-complex. Additionally, the genus Atrichozancla Janse, 1954, syn. nov. hitherto composed of three species (A. phaeocrossis (Meyrick, 1937), A. cosymbota (Meyrick, 1920), and A. gymnopalpa Janse, 1963), is synonymized with the genus Eridachtha on the basis of wing venation and genital characteristics. The adults and genitalia of the new Afrotropical species are illustrated, as well as those of E. calamopis Meyrick, 1920 and E. phaeochlora Meyrick, 1920.

New faunistic and taxonomic data on oribatid mites (Acari, Oribatida) of Ethiopia: results of the joint Russian-Ethiopian biological expedition in Arsi Mountains National Park

ABSTRACT The present study is based on oribatid mite materials collected from Arsi Mountains National Park, Ethiopia, in 2021. A list of 43 species, belonging to 29 genera and 22 families, is presented; of these, three species (Scutovertex evansi, Scheloribates rugiceps and Pilogalumna arabica) are reported for the first time in the Ethiopian fauna. One new species—Antarctozetes behanpelletierae Ermilov sp. nov. (Punctoribatidae) – is described. An identification key to the known species of Antarctozetes from the Afrotropical region is provided. http://www.zoobank.org/rurn:lsid:zoobank.org:pub:A480AE7B-B8BD-441B-A39A-4DBD21C3ED66

Determinants of Farmers’ Perceptions towards Socioecological Benefits of Agroforestry Practices in Northwestern Ethiopia

Agroforestry practices provide multiple ecosystem services in agricultural landscapes. However, within the local context, local communities hold divergent perceptions regarding the various roles of agroforestry, particularly in the Afrotropical Region. Hence, understanding these drivers is critical for adopting and promoting sustainable agroforestry practices. Here, we examined the factors that influenced farmers’ perceptions towards the socioecological benefits of agroforestry practices. A multistage stratified random sampling technique was applied to select 90 households along an urban-rural gradient. The data were gathered through semi-structured questionnaires and key informant interviews and analyzed using a descriptive statistics, chi-square tests, general linear model, and redundancy analysis with the help of R software version 4.3.2. The findings showed that approximately 69.7% of the respondents strongly agreed that agroforestry practices had benefits for society and the environment. However, 11.1% were neutral, and the others disagreed. The results of the general linear model analysis showed that household age, agricultural experience, access to land certification books, and training on tree conservation had a significantly positive effect on farmers’ perceptions of the advantages of agroforestry practices. Furthermore, farmers’ perceptions of the benefits of agroforestry in terms of income generation, educational and cultural values, and climate change mitigation were dependent on household age and farming experience. Overall, this study provides useful insights into the drivers of farmers’ perceptions of the advantages of agroforestry practices in Northwest Ethiopia. The findings of this study underscore the need for policymakers and practitioners to consider sociodemographic and institutional factors that influence farmers’ perceptions when developing policies and plans to advance the adoption and promote sustainable management of agroforestry practices. This supports the widespread adoption of agroforestry practices in tropical agroecosystems.

Myrmecofauna (Hymenoptera, Formicidae) of Gökçeada Island, Türkiye

Türkiye is a very important country for faunal and floral studies due to its geographical location, the zoogeographic importance of its location and the biological richness it brings. This important area has been the subject of research on ants since second half of 19th century. However, studies on the ant fauna of the islands of Türkiye are very old and insufficient. For this reason, the ant fauna of Gökçeada, the largest island of Türkiye, was investigated by direct collecting and litter sifting methods in 2017-2018. 72 species belonging to 4 subfamilies and 23 genera were identified from 1105 ant samples obtained from 73 different localities and 16 different habitats. Among these species, Messor maculifrons Santschi, 1927 and Tetramorium sahlbergi Finzi, 1936 are new records for the ant fauna of Türkiye. In addition, the ergatoid queen of Hypoponera eduardi (Forel, 1894) was recorded for the first time outside the Afrotropical region.

Effective coverage of antenatal care services in Ethiopia: a population-based cross-sectional study

BackgroundAntenatal care (ANC) is a principal component of safe motherhood and reproductive health strategies across the continuum of care. Although the coverage of antenatal care visits has increased in Ethiopia, there needs to be more evidence of effective coverage of antenatal care. The 'effective coverage' concept can pinpoint where action is required to improve high-quality coverage in Ethiopia. Effective coverage indicates a health system's performance by incorporating need, utilization, and quality into a single measurement. The concept includes the number of contacts, facility readiness, interventions received, and components of services received. This study aimed to measure effective antenatal care coverage in Ethiopia.MethodsA two-stage cluster sampling method was used and included 2714 women aged 15–49 years and 462 health facilities from six Ethiopian regions from October 2019 to January 2020. The effective coverage cascade was analyzed among the targeted women by computing the proportion who received four or more antenatal care visits where the necessary inputs were available, received iron-folate supplementation and two doses of tetanus vaccination according to process quality components of antenatal care services.ResultsOf all women, 40% (95%CI; 38, 43) had four or more visits, ranging from 3% in Afar to 74% in Addis Ababa. The overall mean health facility readiness score of the facilities serving these women was 70%, the vaccination and iron-folate supplementation coverage was 26%, and the ANC process quality was 64%. As reported by women, the least score was given to the quality component of discussing birth preparedness and complication readiness with providers. In the effective coverage cascade, the input-adjusted, intervention-adjusted, and quality-adjusted antenatal coverage estimates were 28%, 18%, and 12%, respectively.ConclusionThe overall effective ANC coverage was low, primarily due to a considerable drop in the proportion of women who completed four or more ANC visits. Improving quality of services is crucial to increase ANC up take and completion of the recommended visits along with interventions increasing women’s awareness.

Unleashing student potential through co-curricular activities in East Hararghe Secondary Schools, Ethiopia

Co-curricular activities are essential for students' overall development, offering valuable life skills, personal interest exploration, and enriching educational experiences. This study aimed at to explore the effect of co-curricular activities on students' holistic development and education quality. Data was gathered from eight supervisors, ten principals, 14 vice-principals, 20 co-curricular coordinators, 109 teachers, and 259 students across ten schools through purposive and stratified random sampling techniques. A questionnaire and interviews were employed for data collection. Both descriptive and inferential statistics were applied for analysis. The results revealed that co-curricular activities in secondary schools are currently deficient in terms of quantity and quality. Issues such as disorganization, structural challenges, limited student participation, and inadequate training contribute to this problem. Consequently, these activities have limited influence on students' overall development and education quality. The study's implications extend beyond the specific Ethiopian region, highlighting the potential of co-curricular activities to unlock students' potential.

Species Diversity of the Leafhopper Tribe Paralimnini Distant (Hemiptera: Cicadellidae: Deltocephalinae) from Pakistan, with Description of Two New Genera

Abstract Paralimnini Distant, 1908, is a notable leafhopper tribe that exhibits a wide distribution across all biogeographical regions, namely the Palearctic, Nearctic, Afrotropical, Oriental, Neotropical, and Australian regions. Among these, the Oriental region demonstrates the highest diversity of Paralimnini, with a significant portion of this diversity remaining unexplored according to recent investigations. This study focuses on examining 10 species under seven genera of the Paralimnini leafhopper tribe found in Pakistan. A comprehensive morphological study is conducted, which includes the description of two new genera, Paralimnolus gen. nov. and Lalianlus gen. nov., with P. bicolor sp. nov. and L. bifurcates sp. nov. within the tribe. The study meticulously describes and provides illustrations of all the taxa based on careful observations of specimens obtained from Pakistan. Additionally, the study furnishes an updated checklist and a key to facilitate the identification of known and new Pakistani genera belonging to the tribe Paralimnini. The present findings shed light on the diversity and distribution patterns of the Paralimnini tribe in Pakistan, particularly emphasizing the junction of the Oriental and Palearctic regions in the northern parts, as these parts of the country serve as a significant reservoir of undocumented species. The thorough documentation and available identification resources provided in this study serve as valuable references for future investigations and contribute to our knowledge of the leafhopper fauna in Pakistan.

Low coverage of species constrains the use of DNA barcoding to assess mosquito biodiversity

Mosquitoes (Culicidae) represent the main vector insects globally, and they also inhabit many of the terrestrial and aquatic habitats of the world. DNA barcoding and metabarcoding are now widely used in both research and routine practices involving mosquitoes. However, these methodologies rely on information available in databases consisting of barcode sequences representing taxonomically identified voucher specimens. In this study, we assess the availability of public data for mosquitoes in the main online databases, focusing specifically on the two most widely used DNA barcoding markers in Culicidae: COI and ITS2. In addition, we test hypotheses on possible factors affecting species coverage (i.e., the percentage of species covered in the online databases) for COI in different countries and the occurrence of the DNA barcode gap for COI. Our findings showed differences in the data publicly available in the repositories, with a taxonomic or species coverage of 28.4–30.11% for COI in BOLD + GenBank, and 12.32% for ITS2 in GenBank. Afrotropical, Australian and Oriental biogeographic regions had the lowest coverages, while Nearctic, Palearctic and Oceanian had the highest. The Neotropical region had an intermediate coverage. In general, countries with a higher diversity of mosquitoes and higher numbers of medically important species had lower coverage. Moreover, countries with a higher number of endemic species tended to have a higher coverage. Although our DNA barcode gap analyses suggested that the species boundaries need to be revised in half of the mosquito species available in the databases, additional data must be gathered to confirm these results and to allow explaining the occurrence of the DNA barcode gap. We hope this study can help guide regional species inventories of mosquitoes and the completion of a publicly available reference library of DNA barcodes for all mosquito species.

HarvestNet: A Dataset for Detecting Smallholder Farming Activity Using Harvest Piles and Remote Sensing

Small farms contribute to a large share of the productive land in developing countries. In regions such as sub-Saharan Africa, where 80% of farms are small (under 2 ha in size), the task of mapping smallholder cropland is an important part of tracking sustainability measures such as crop productivity. However, the visually diverse and nuanced appearance of small farms has limited the effectiveness of traditional approaches to cropland mapping. Here we introduce a new approach based on the detection of harvest piles characteristic of many smallholder systems throughout the world. We present HarvestNet, a dataset for mapping the presence of farms in the Ethiopian regions of Tigray and Amhara during 2020-2023, collected using expert knowledge and satellite images, totalling 7k hand-labeled images and 2k ground-collected labels. We also benchmark a set of baselines, including SOTA models in remote sensing, with our best models having around 80% classification performance on hand labelled data and 90% and 98% accuracy on ground truth data for Tigray and Amhara, respectively. We also perform a visual comparison with a widely used pre-existing coverage map and show that our model detects an extra 56,621 hectares of cropland in Tigray. We conclude that remote sensing of harvest piles can contribute to more timely and accurate cropland assessments in food insecure regions. The dataset can be accessed through https://figshare.com/s/45a7b45556b90a9a11d2, while the code for the dataset and benchmarks is publicly available at https://github.com/jonxuxu/harvest-piles

New insights into the molecular phylogeny, biogeographical history, and diversification of Amblyomma ticks (Acari: Ixodidae) based on mitogenomes and nuclear sequences.

Amblyomma is the third most diversified genus of Ixodidae that is distributed across the Indomalayan, Afrotropical, Australasian (IAA), Nearctic and Neotropical biogeographic ecoregions, reaching in the Neotropic its highest diversity. There have been hints in previously published phylogenetic trees from mitochondrial genome, nuclear rRNA, from combinations of both and morphology that the Australasian Amblyomma or the Australasian Amblyomma plus the Amblyomma species from the southern cone of South America, might be sister-group to the Amblyomma of the rest of the world. However, a stable phylogenetic framework of Amblyomma for a better understanding of the biogeographic patterns underpinning its diversification is lacking. We used genomic techniques to sequence complete and nearly complete mitochondrial genomes -ca. 15 kbp- as well as the nuclear ribosomal cluster -ca. 8 kbp- for 17 Amblyomma ticks in order to study the phylogeny and biogeographic pattern of the genus Amblyomma, with particular emphasis on the Neotropical region. The new genomic information generated here together with genomic information available on 43 ticks (22 other Amblyomma species and 21 other hard ticks-as outgroup-) were used to perform probabilistic methods of phylogenetic and biogeographic inferences and time-tree estimation using biogeographic dates. In the present paper, we present the strongest evidence yet that Australasian Amblyomma may indeed be the sister-group to the Amblyomma of the rest of the world (species that occur mainly in the Neotropical and Afrotropical zoogeographic regions). Our results showed that all Amblyomma subgenera (Cernyomma, Anastosiella, Xiphiastor, Adenopleura, Aponomma and Dermiomma) are not monophyletic, except for Walkeriana and Amblyomma. Likewise, our best biogeographic scenario supports the origin of Amblyomma and its posterior diversification in the southern hemisphere at 47.8 and 36.8 Mya, respectively. This diversification could be associated with the end of the connection of Australasia and Neotropical ecoregions by the Antarctic land bridge. Also, the biogeographic analyses let us see the colonization patterns of some neotropical Amblyomma species to the Nearctic. We found strong evidence that the main theater of diversification of Amblyomma was the southern hemisphere, potentially driven by the Antarctic Bridge's intermittent connection in the late Eocene. In addition, the subgeneric classification of Amblyomma lacks evolutionary support. Future studies using denser taxonomic sampling may lead to new findings on the phylogenetic relationships and biogeographic history of Amblyomma genus.

Phylogeny and historical biogeography of the leafhopper subfamily Coelidiinae (Hemiptera: Cicadellidae) based on morphological and molecular data

AbstractThe phylogenetic relationships among major lineages of the globally distributed leafhopper subfamily Coelidiinae were reconstructed by analysis of 2903 nucleotide positions from two mitochondrial genes (COI and 16S), four nuclear genes (28S, H3, H2A and Wingless) and 102 discrete morphological characters, compiled for 86 species representing 52 genera within 9 coelidiine tribes broadly representative of the world fauna in addition to 12 outgroup taxa. Maximum likelihood and Bayesian analyses yielded well‐resolved phylogenetic estimates that were highly congruent with most branches receiving strong support. The results indicate Coelidiinae sensu stricto (=Coelidiinae sensu lato without Equeefini and Macroceratogoniini), Thagriini, Tharrini, Tinobregmini and Equeefini are monophyletic. However, the two largest tribes, Coelidiini and Teruliini, are paraphyletic, as well as Youngolidiini and most genera of Oriental Coelidiini. Fossil‐calibrated molecular divergence time analysis indicates that the Coelidiinae sensu stricto originated ca. 149 Ma, prior to the complete separation of the Gondwanan continents, and the tribe‐level lineages diverged between the Lower and Upper Cretaceous (92.77–138.03 Ma). The major lineages of Coelidiinae arose in the Oriental and Neotropical regions, and Oriental Coelidiini arose as a result of dispersal and colonisation from the Afrotropical region.

Synopsis of Neotropical Trogolaphysa Mills, 1938 (Collembola: Paronellidae) with Reduced Eye Number, and Description of Two New Troglobiontic Species from Belize

The genus Trogolaphysa comprises 81 described species distributed across the Neotropical and Afrotropical regions. In the Americas, just over half of the species found in subterranean habitats have a reduced eye number (0–5 eyes). Subterranean species are of interest as models to study the evolution of morphological adaptations. Many subterranean species of Trogolaphysa were described before the introduction of chaetotaxy as a diagnostic tool and thus remain incompletely described and diagnosed. To identify gaps in descriptions and facilitate the identification of newly collected forms, we provide standardized summary descriptions, species diagnoses, diagnostic tables, and a dichotomous key to the 33 Neotropical species of Trogolaphysa with reduced eye number. As a result of this synthesis, we describe two new troglobiontic species, Trogolaphysa reneaui n. sp. and Trogolaphysa welchi n. sp. from Belizean caves.

New insights into the systematics of the afrotropical Amblyomma marmoreum complex (Acari: Ixodidae) and the genome of a novel Rickettsia africae strain using morphological and metagenomic approaches

The Amblyomma marmoreum complex includes afrotropical species, such as Amblyomma sparsum, a three-host tick that parasitizes reptiles, birds, and mammals, and is a recognized vector of Ehrlichia ruminantium. However, the lack of morphological, genetic and ecological data on A. sparsum has caused considerable confusion in its identification. In this study, we used microscopy and metagenomic approaches to analyze A. sparsum ticks collected from a puff adder snake (Bitis arietans) in southwest Senegal (an endemic rickettsioses area) in order to supplement previous morphological descriptions, provide novel genomic data for the A. marmoreum complex, and describe the genome of a novel spotted fever group Rickettsia strain. Based on stereoscope and scanning electron microscopy (SEM) morphological evaluations, we provide high-quality images and new insights about punctation and enameling in the adult male of A. sparsum to facilitate identification for future studies. The metagenomic approach allowed us assembly the complete mitochondrial genome of A. sparsum, as well as the nearly entire chromosome and complete plasmid sequences of a novel Rickettsia africae strain. Phylogenomic analyses demonstrated a close relationship between A. sparsum and Amblyomma nuttalli for the first time and confirmed the position of A. sparsum within the A. marmoreum complex. Our results provide new insights into the systematics of A. sparsum and A. marmoreum complex, as well as the genetic diversity of R. africae in the Afrotropical region. Future studies should consider the possibility that A. sparsum may be a vector for R. africae.

Regional disparities in antenatal care utilization among pregnant women and its determinants in Ethiopia.

Antenatal care primarily focuses on health care checkups, the provision of advice on healthy behaviors, and the delivery of psychological, social, and emotional support for women with pregnancy. The national target set by the Ethiopian government is to achieve 95% of at least four ANC visits. Nevertheless, 43.11% of women had four or more checkups, according to the 2019 Ethiopian Demographic and Health Survey. Despite this achievement, antenatal care visits differ significantly between Ethiopian regions. Consequently, the purpose of this study was to assess regional disparities in pregnant women's utilization of antenatal care and its determinants in Ethiopia. We have used 2019 intermediate Ethiopian Demographic and Health Survey data for analysis. The analysis comprised a total of 3,917 weighted women age 15-49 who had a live birth in the 5 years preceding the survey. Poisson regression analysis was done using SAS software version 9.4. To show the strength and direction of the association, an incidence rate ratio with a 95% confidence interval was used. Variables with a p-value <0.05 were declared as significant factors associated with the number of ANC visits. In Ethiopia, the number of ANC visits differs between regions. With a mean of 4.74 (95% CI: 4.49, 4.99), Addis Ababa reported the highest percentage of ANC visits (82.7%), while the Somali Region reported the lowest percentage (11.3%) with a mean of 0.73 (95% CI: 0.57, 0.88). Maternal age, educational level, religion, household wealth index, place of delivery, and household size show significant associations with the number of antenatal care visits. In Ethiopia, there is the highest regional disparity in the number of ANC visits. The number of ANC visits was influenced by the mother's age, education, religion, household wealth index, place of delivery, and household size. Regarding the ANC visits, there should be initiatives that address the demands of pastoralist and agro-pastoralist communities to increase ANC utilization. As with many other health outcomes, education and low socio-economic status were associated with low ANC visit but these are tied to the overall social development of a country and are not immediately amenable to public health interventions.

Insularity and its impact on stingless bee honey properties: A case study in the Zanzibar Archipelago (Tanzania)

AbstractBackgroundIslands are known to host unique flowering plant assemblages visited by insular insects, including by social bees, a phenomenon thought to potentially impact the honey composition and associated medicinal applications. In this study, we characterized several physical–chemical parameters of honey produced by the stingless bee Meliponula (Axestotrigona) ferruginea with samples collected both from continental (Kenya) and insular (Tanzania) ecosystems.ResultsOur results show that stingless bees in islands produce a honey whose composition significantly differs from those produced in the continent, with a significantly higher concentration of bioactive phytochemicals.ConclusionOur results illustrate how insularity might drive different and unique compositional profiles in honeys and provide evidence that certain groups of compounds found in stingless bee honey from the Afrotropical region have potential therapeutic benefits with medicinal applications that largely remain to be explored.

A new species of the genus Euxiphocerus (Diptera, Dolichopodidae) from Korea with checklist and key to species of the genus.

Euxiphocerus Parent, 1935 is a small genus consisting of three species only from the Afrotropical region. During a survey of the long-legged flies from Korea, the authors discovered a new species, Euxiphoceruslignicola sp. nov. Detailed morphological characters and photographs of the new species, as well as a checklist and key to species of this genus, are provided herein. The two species described from Oriental China, Systenusjinxiuensis Lin & Yang, 2022 and S.sinensis Yang & Gaimari, 2004, are transferred to Euxiphocerus Parent, 1935 (comb. nov.) by similarity in external morphological characters.

New species and records of Afrotropical Campopleginae V. (Hymenoptera: Ichneumonidae)

Two new species of Afrotropical Campopleginae (Hymenoptera: Ichneumonidae) are described: Olesicampe africana sp. nov. from Guinea, and Sinophorus runei sp. nov. from South Africa, representing the first species of both genera described from the Afrotropical region, and, for the latter, the first species described from the Southern Hemisphere. Additionally, Dusona pauliani (Benoit, 1957), a species previously known from Réunion only, is reported for the first time from South Africa.

Geographical variation in hotspots of stunting among under-five children in Ethiopia: A geographically weighted regression and multilevel robust Poisson regression analysis.

Childhood stunting is a global public health concern, associated with both short and long-term consequences, including high child morbidity and mortality, poor development and learning capacity, increased vulnerability for infectious and non-infectious disease. The prevalence of stunting varies significantly throughout Ethiopian regions. Therefore, this study aimed to assess the geographical variation in predictors of stunting among children under the age of five in Ethiopia using 2019 Ethiopian Demographic and Health Survey. The current analysis was based on data from the 2019 mini Ethiopian Demographic and Health Survey (EDHS). A total of 5,490 children under the age of five were included in the weighted sample. Descriptive and inferential analysis was done using STATA 17. For the spatial analysis, ArcGIS 10.7 were used. Spatial regression was used to identify the variables associated with stunting hotspots, and adjusted R2 and Corrected Akaike Information Criteria (AICc) were used to compare the models. As the prevalence of stunting was over 10%, a multilevel robust Poisson regression was conducted. In the bivariable analysis, variables having a p-value < 0.2 were considered for the multivariable analysis. In the multivariable multilevel robust Poisson regression analysis, the adjusted prevalence ratio with the 95% confidence interval is presented to show the statistical significance and strength of the association. The prevalence of stunting was 33.58% (95%CI: 32.34%, 34.84%) with a clustered geographic pattern (Moran's I = 0.40, p<0.001). significant hotspot areas of stunting were identified in the west and south Afar, Tigray, Amhara and east SNNPR regions. In the local model, no maternal education, poverty, child age 6-23 months and male headed household were predictors associated with spatial variation of stunting among under five children in Ethiopia. In the multivariable multilevel robust Poisson regression the prevalence of stunting among children whose mother's age is >40 (APR = 0.74, 95%CI: 0.55, 0.99). Children whose mother had secondary (APR = 0.74, 95%CI: 0.60, 0.91) and higher (APR = 0.61, 95%CI: 0.44, 0.84) educational status, household wealth status (APR = 0.87, 95%CI: 0.76, 0.99), child aged 6-23 months (APR = 1.87, 95%CI: 1.53, 2.28) were all significantly associated with stunting. In Ethiopia, under-five children suffering from stunting have been found to exhibit a spatially clustered pattern. Maternal education, wealth index, birth interval and child age were determining factors of spatial variation of stunting. As a result, a detailed map of stunting hotspots and determinants among children under the age of five aid program planners and decision-makers in designing targeted public health measures.