African Lions Panthera Leo Research Articles

Equal contributions of feline immunodeficiency virus and coinfections to morbidity in African lions.

Feline immunodeficiency virus (FIV) is a pathogenic lentivirus related to human and simian immunodeficiency viruses that has been associated with AIDS-like pathologies in domestic and wild cats, as well as in hyenas. Despite known pathologies, progressive immunosuppression and ill health effects driven by these lentiviruses in association with other secondary infections remain understudied in free-ranging species. Here, the role of coinfections by gastrointestinal parasites and tick-borne hemoparasites for FIV disease progression was explored in 195 free-ranging African lions (Panthera leo) living in Kruger National Park (KNP), South Africa. Using statistical methodology, we evaluated the effects of FIV on a range of health indicators to explore how direct and indirect effects of FIV and associated coinfections align to determine lion health outcomes. Findings show direct negative effects of FIV on host immunity and nutritional status, and exacerbation of aggressive behaviors, conditions which may increase exposure/susceptibility to other secondary infections. When taken together, the contribution of coinfecting parasites to morbidity in lions is of similar magnitude as direct effects of FIV infection alone, suggesting that the particular coinfection assemblage may play a role in mediating disease progression within natural lion populations.

Room to roam for African lions Panthera leo: a review of the key drivers of lion habitat use and implications for conservation

Abstract Globally, large terrestrial carnivores (Carnivora) have suffered precipitous declines in population and range. Today, they must persist in increasingly isolated natural habitat patches within a human‐dominated matrix. Effective conservation aimed at supporting carnivores in such landscapes requires species‐specific understanding of habitat requirements. We present results from a review of the published literature to assess the current state of knowledge regarding habitat preferences of the African lion Panthera leo, with the aim of identifying common drivers of habitat use across contexts. Using the Web of Science, we identified 154 usable articles and extracted information relating to study topic, location, habitats described, land‐use type, and any documented habitat preferences. Only 31 studies documented evidence of habitat use, and collectively, they suggested that preferences for specific habitat types were varied and context‐specific. The importance of prey abundance and proximity to water was highlighted in multiple studies. Anthropogenic factors interfered with expected patterns of habitat use. There was evident bias in study locations: 83% of the habitat‐use studies were based in only three countries, and 70% were focussed on protected or managed areas. Our synthesis suggests that lions demonstrate behavioural plasticity in habitat use in response to anthropogenic pressures. To understand the limits of this plasticity and to manage Africa’s changing landscapes effectively for roaming lions, future research should be focussed on analysis of habitat use outside protected areas, taking into account gradients of distance to water, prey abundance, and anthropogenic risk.

Dead-reckoning animal movements in R: a reappraisal using Gundog.Tracks

BackgroundFine-scale data on animal position are increasingly enabling us to understand the details of animal movement ecology and dead-reckoning, a technique integrating motion sensor-derived information on heading and speed, can be used to reconstruct fine-scale movement paths at sub-second resolution, irrespective of the environment. On its own however, the dead-reckoning process is prone to cumulative errors, so that position estimates quickly become uncoupled from true location. Periodic ground-truthing with aligned location data (e.g., from global positioning technology) can correct for this drift between Verified Positions (VPs). We present step-by-step instructions for implementing Verified Position Correction (VPC) dead-reckoning in R using the tilt-compensated compass method, accompanied by the mathematical protocols underlying the code and improvements and extensions of this technique to reduce the trade-off between VPC rate and dead-reckoning accuracy. These protocols are all built into a user-friendly, fully annotated VPC dead-reckoning R function; Gundog.Tracks, with multi-functionality to reconstruct animal movement paths across terrestrial, aquatic, and aerial systems, provided within the Additional file 4 as well as online (GitHub).ResultsThe Gundog.Tracks function is demonstrated on three contrasting model species (the African lion Panthera leo, the Magellanic penguin Spheniscus magellanicus, and the Imperial cormorant Leucocarbo atriceps) moving on land, in water and in air. We show the effect of uncorrected errors in speed estimations, heading inaccuracies and infrequent VPC rate and demonstrate how these issues can be addressed.ConclusionsThe function provided will allow anyone familiar with R to dead-reckon animal tracks readily and accurately, as the key complex issues are dealt with by Gundog.Tracks. This will help the community to consider and implement a valuable, but often overlooked method of reconstructing high-resolution animal movement paths across diverse species and systems without requiring a bespoke application.

Evaluation of Fecal Glucocorticoid Metabolite Levels in Response to a Change in Social and Handling Conditions in African Lions (Panthera leo bleyenberghi).

Simple SummaryNon-invasive determination of cortisol metabolite concentrations in feces is widely used to evaluate the influence of housing and handling conditions on the stress physiology of wildlife in captivity. The present study aimed to assess the physiological response of a lion pride to a change in management and social conditions after the death of the dominant male of the pride. Before the dominant male died, weekly management routines between the indoor and outdoor enclosures were conducted to avoid cohabitation problems between the two males of the pride. After the death of the dominant male, these weekly management dynamics ceased, leading to a decrease in the daily management routine of the lion pride. An individualized sampling of the animals through the utilization of indigestive markers was conducted, and fecal samples were collected before and after the death of the dominant male. Significant lower cortisol metabolite concentrations in feces were detected after the death of the dominant male, suggesting a positive impact of a decrease in daily management routines, together with a more stable social environment. In addition, assessment of individualized hormone concentrations throughout the study revealed variable physiological responses among lions, providing evidence of the importance of monitoring hormonal profiles individually.Monitoring the hypothalamic–pituitary–adrenal (HPA) axis through determination of fecal cortisol metabolite (FCM) levels is a non-invasive method useful for understanding how handling and social conditions may affect the physiological status of zoo animals. The present study used FCM analysis to evaluate whether the HPA axis activity of a lion pride was modified by a change in social and handling conditions after the death of the dominant male. Five African lions (Panthera leo bleyenberghi), two males and three females, were included in the study. Fecal samples were collected before and after the death of the dominant male. To avoid cohabitation conflicts between males before the dominant male died, subgroups were established and subjected to weekly changes between indoor and outdoor facilities. After the death of the dominant male, these management dynamics ceased, and the remaining four lions were kept together outdoors. Significant lower group FCM concentrations (p < 0.001) were detected after the decease of the dominant male, probably associated with a decrease in daily handling, together with a more stable social environment. Overall, the present study indicates the effect of different management scenarios on the HPA axis activity and differentiated physiological responses to the same situation between individuals.

African Wild Dog Movement Ecology in a Small Protected Area in South Africa

Dramatic population declines of African wild dogs (Lycaon pictus) led to a managed metapopulation approach for wild dog conservation in South Africa. Monitoring the survival and habitat use of packs reintroduced into protected areas (PAs) is an essential part of adaptive management and improving the health and, ultimately, the survival of the metapopulation. Our study describes the territoriality and habitat selection of a pack of wild dogs reintroduced into Manyoni Private Game Reserve (219 km2) in northern KwaZulu-Natal, South Africa. Despite being introduced into a small PA, the pack only utilized half their available space (121 km2) and avoided the central areas of the reserve. Post hoc analysis of African lion (Panthera leo) localities suggested competitive avoidance was a strong factor in shaping the habitat usage of the pack; however, further research is required. Habitat selection also varied seasonally and with denning. Ultimately, we showed that spatio-temporal analyses can help identify high-risk areas within wild dog territories, such as hotspots of activity along fencelines. These analyses can then be used to increase targeted management of these areas, such as improving the maintenance of well-used fencelines, which is an important consideration for the sustained success of the metapopulation across small PAs.

Ending Commercial Lion Farming in South Africa: A Gap Analysis Approach.

Simple SummaryIn South Africa, African lions (Panthera leo) are bred on farms for commercial purposes such as tourism, trophy hunting, and the international traditional medicine market. Despite its legal status, South Africa’s growing lion farming industry is a contentious issue. In 2020, a high-level panel was appointed to review the policies, legislation, and management of breeding, hunting, trade, and handling of four wildlife species, namely rhino, elephant, leopard, and lions. In May 2021, it was announced that the government will stop issuing permits to new entrants into this industry as well as the issuance of hunting permits and will start amending permit conditions to prohibit breeding and exclude tourism interactions with captive lions, effectively ending the lion farming industry. In order to follow this line of action, a comprehensive, well-managed plan will be required to ensure a responsible transition away from the current industry. Here, using a “gap analysis” management tool, we outline some of the key considerations necessary for a responsible, well-managed exit from the lion farming industry in South Africa. We compiled key background information about the current state of the industry and use this information to identify desired management states and specific steps that could facilitate a successful phase out of lion farming.African lions (Panthera leo) are commercially farmed across South Africa for sport hunting, tourism, and the international bone trade, primarily in Southeast Asia. Despite its legal status, South Africa’s growing lion farming industry is a contentious issue. In 2020 a high-level panel was initiated to review the policies, legislation, and management regarding the breeding, hunting, trade, and handling of four wildlife species, including lions. In May 2021, it was announced that the government intends to amend existing permit conditions to prohibit lion breeding and tourism interactions with captive lions, as well as to stop issuing permits to new entrants into the industry, effectively ending lion farming. In order to follow this line of action, a comprehensive, well-managed plan will be necessary to execute a responsible exit from the industry as it currently stands. Using a “gap analysis” management tool, we aim to: (1) outline some of the key considerations regarding the current state of the lion farming industry in South Africa; and (2) propose specific action steps that could be taken within five key areas (regulation, animal welfare, health and safety, equitability, and conservation) to help inform a responsible transition away from this type of wildlife farming in the biodiversity economy. For our gap analysis, we conducted a semi-systematic literature search to compile key background information about the current state of the industry. This information was then used to identify corresponding desired management states, and steps that could facilitate a successful phase out of lion farming in South Africa. We hope our approach helps identify key considerations for a responsible transition and can help aid decisions during the management of this process.

A pan-African spatial assessment of human conflicts with lions and elephants

African lions (Panthera leo) and African savanna (Loxodonta africana) and forest (L. cyclotis) elephants pose threats to people, crops, and livestock, and are themselves threatened with extinction. Here, we map these human-wildlife conflicts across Africa. Eighty-two percent of sites containing lions and elephants are adjacent to areas with considerable human pressure. Areas at severe risk of conflict (defined as high densities of humans, crops, and cattle) comprise 9% of the perimeter of these species’ ranges and are found in 18 countries hosting, respectively, ~ 74% and 41% of African lion and elephant populations. Although a variety of alternative conflict-mitigation strategies could be deployed, we focus on assessing the potential of high-quality mitigation fences. Our spatial and economic assessments suggest that investments in the construction and maintenance of strategically located mitigation fences would be a cost-effective strategy to support local communities, protect people from dangerous wildlife, and prevent further declines in lion and elephant populations.

Monitoring compliance of CITES lion bone exports from South Africa.

From 2008 to 2018, South Africa permitted the export of captive-bred African lion (Panthera leo) skeletons to Southeast Asia under CITES Appendix II. Legal exports rose from approximately 50 individuals in 2008 to a maximum of 1,771 skeletons in 2016, and has led to ongoing concerns over possible laundering of non-lion, multiple-source and wild-sourced bones. South Africa is required under its obligations to CITES to employ mechanisms for monitoring and reporting trade, and to limit the potential for illegal trade and laundering of lion and other large felid bones. Monitoring tools for legal trade are critical to compliance with CITES. Here we evaluate the CITES-compliance procedure implemented by South Africa for export of lion bones and identify six essential general points for consideration in the implementation of animal export quota compliance protocols. We provide specific insight into the South African lion bone export monitoring system through: i) outlining the protocols followed; ii) assessing the utility of cranial morphology to identify species; iii) evaluating skeleton consignment weight as a monitoring tool; and iv) presenting molecular (DNA) species assignment and pairwise-comparative sample matching of individuals. We describe irregularities and illicit behaviour detected in the 2017 and 2018 lion bone quotas. Notably, we report that the compliance procedure successfully identified and prevented the attempted laundering of a tiger (P. tigris) skeleton in 2018. We emphasise the utility of mixed-method protocols for the monitoring of compliance in CITES Appendix II export quota systems.

FIRST REPORT OF ENTEROCYTOZOON BIENEUSI FROM AN AFRICAN LION (PANTHERA LEO) IN A ZOO IN THE REPUBLIC OF KOREA.

Enterocytozoon bieneusi is the most common species of microsporidia that infects humans and animals worldwide. However, no information is available on E. bieneusi infection among zoo animals in the Republic of Korea (ROK). Here, we investigated the prevalence of E. bieneusi among animals kept in zoos and the zoonotic potential of the E. bieneusi identified. E. bieneusi was detected only in one African lion (Panthera leo) with diarrhea, using PCR and sequencing analysis of the internal transcribed spacer (ITS) of the rRNA gene. A phylogenetic analysis based on the ITS gene showed that the lion isolate was classified into a novel genotype KPL belonging to Group 2. The KPL genotype identified in this study differed from genotype I in 6 nucleotides and from genotype I-like in 3 nucleotides, respectively, indicating that Group 2 has the capacity to infect a wide range of hosts. This is the first report of the presence of E. bieneusi in an African lion housed in a zoo in the ROK. Further investigation is necessary to study E. bieneusi infection among zoo animals in various regions and to determine the transmission route, in order to control E. bieneusi infection.

Cluster analysis in ethological research

Big cats are often on display in zoos around the world. The study of their time budget is the basis of ethological research in captivity. The paper considers the features of the behavior of the subfamily Pantherinae, the daily activity of animals in the summer, methods of keeping, the exposition of enclosures, and relationships with keepers. The studies were conducted in the summer of 2012 and 2013 at the Barnaul Zoo. The total observation time for the animals was 120 hours. The behavior of the African lion (Panthera leo leo – male), the Ussuri tiger (Panthera tigris altaica – female), and the Amur leopard (Panthera pardus orientalis – male) has been studied. In the course of the work, the compilation of ethograms, continuous recording, and free observations were used. The clustering method was applied to analyze the patterns of behavior of animals in captivity. Cluster analysis breaks down the behavior of captivities animals into two large blocks. Locomotion in animals should be considered as a separate block. The animal’s growth and development period require a high proportion of physical activity, which is noticeable when observing the Amur tiger. Locomotion occupied 32.8% of the total time budget of this animal. Large cats have never been in a shelter (in wooden structures of the appropriate size). They used the roof of the houses only as a place for rest and observation. The proportion of marking, hunting, eating, exploratory behavior, grooming, and such forms of behavior as freezing, static position, orienting reaction did not differ significantly. Play behavior with elements of hunting and manipulative activity took 5.5% of the Amur tiger’s time budget for the period under review. We associate this primarily with the age of the given animal. Play behavior was observed two times less often in the Far Eastern leopard (2.9%) and African lion (2.6%)..

Harassment‐induced changes in lion space use as a conflict mitigation tool

AbstractHuman‐wildlife conflict represents a substantial threat to rural livelihoods and species persistence. Directed harassment (i.e., hazing) is one method for mitigating conflict, though gauging its effectiveness is often complicated by a lack of replication, controls, or effective contrasts. Herein we assessed whether African lions (Panthera leo) shifted their space use in response to a hazing program intended to deter lions from community lands adjacent to Hwange National Park, Zimbabwe. Using GPS‐collared lions (n = 16) from unique prides, we investigated the degree to which individuals exhibited (a) a large‐scale response by shifting their home ranges away from community lands, or (b) a fine‐scale response by increasingly avoiding areas near households post‐program initiation. Responses between “at‐risk” (those exposed to the program; n = 5) and “protected” (n = 11) lions varied, with at‐risk males shifting their home ranges to include 12.08% more national park and 9.04% less community lands post‐program (median), a shift not replicated by at‐risk females nor protected lions. However, the majority of at‐risk lions demonstrated increased attraction to households post‐program. We demonstrated that while aversive stimulus approaches may alter large‐scale space use by individual lions, constraints imposed by social structure and available habitat alternatives will affect the level of effort required to achieve program success.

Dynamic interactions between apex predators reveal contrasting seasonal attraction patterns.

Apex predators play important roles in ecosystem functioning and, where they coexist, intraguild interactions can have profound effects on trophic relationships. Interactions between predators range from intraguild predation and competition to facilitation through scavenging opportunities. Despite the increased availability of fine-scale GPS data, the determinants and outcomes of encounters between apex predators remain understudied. We used simultaneous GPS data from collared spotted hyaenas (Crocuta crocuta) and African lions (Panthera leo) in Hwange National Park, Zimbabwe, to determine the environmental conditions of the encounters between the two species, which species provoked the encounter, and which species dominated the encounter. Our results show that encounters between hyaenas and lions are mostly resource-related (over a carcass or around waterholes). In the wet season, encounters mainly occur at a carcass, with lions being dominant over its access. In the dry season, encounters mainly occur in the absence of a carcass and near waterholes. Movements of hyaenas and lions before, during, and after these dry-season encounters suggest two interference scenarios: a passive interference scenario whereby both predators would beattracted to waterholes but lionswould leave a waterhole used by hyaenas because of prey disturbance, and an active interference scenario whereby hyaenas would actively chase lions from waterhole areas, which are prime hunting grounds. This study highlights the seasonal dynamics of predator interactions and illustrates how the relative importance of negative interactions (interference competition during the dry season) and positive interactions (scavenging opportunities during the wet season) shifts over the course of the year.

Gastrointestinal Helminths in Large Felines from a Zoo in Malaysia

In nature, wild animals live in an enormous space and usually have very low genetic resistance against parasitic infection mainly due to low exposure towards the parasites themselves. However, when herds of these wild animals are kept in captivity, or in zoological gardens, parasitic infections might be worse and pose a serious threat to endangered species. The present study was conducted to observe the occurrence of gastrointestinal parasites in large felines in a Malaysian zoo. Ten faecal samples were collected from pumas (Puma concolor, n = 5), African lions (Panthera leo, n = 3), a spotted leopard (Panthera pardus, n = 1), and a black panther (Panthera onca, n = 1). All faecal samples were examined for parasite eggs, larvae, and oocysts by simple faecal floatation and formalin – ether sedimentation technique. All large felines in the zoo were infected with gastrointestinal parasites. A total of six species of gastrointestinal parasites were recovered including four nematodes (Toxocara cati, Ancylostoma spp., Toxascaris leonina, and Oxyuris sp.), a cestode (Spirometra sp.), and a protozoan (Isospora sp.). Half (n=5/10) of the large felines had mixed infections with Toxocara cati and Ancylostoma spp.

Do pastoralist cattle fear African lions?

Fear of predators fundamentally shapes the ecology of prey species and drives both inter‐ and intra‐specific interactions. Extensive research has examined the consequences of predation risk from large carnivores on the behavior of wild ungulate prey species. However, many large carnivores not only hunt wild prey but also depredate domestic livestock, especially in pastoralist systems where livestock share land and resources with large carnivores. Northern Tanzania is a hotspot for human–carnivore conflict driven by livestock depredation and interactions are particularly severe between African lions Panthera leo and pastoralist cattle Bos taurus. In this ecosystem, we explored the degree to which pastoralist cattle exhibited anti‐predator behaviors during their daily grazing routines. Using focal animal sampling, we compared two typical anti‐predator behaviors, vigilance and grouping, among cattle in village rangelands with high and low background depredation rates. We found that cattle in high risk village rangelands formed 21.2% larger groups than cattle in low risk village rangelands. Interestingly, cattle in low risk village rangelands spent 68.4% more time vigilant than cattle in high risk village rangelands. These patterns were influenced significantly by the time of day: as sunset approached, cattle in low risk village rangelands spent more time vigilant and cattle in high risk village rangelands formed larger groups. These results suggest that pastoralist cattle exhibit anti‐predator strategies that vary both spatially and temporally, and that such strategies might help livestock optimally tradeoff the costs and benefits of anti‐predator behavior across timescales (i.e. the risk allocation hypothesis). We discuss the implications of our results for husbandry techniques that might reduce behavioral costs associated with cattle anti‐predator behaviors and help increase tolerance for lions and other large carnivores. These improvements are critical to human–carnivore coexistence given the prevalence of pastoralism globally and the rising potential for conflict with large carnivores such as lions.

Understanding the dynamics of lion attacks on humans and livestock in southern Maasailand, Kenya

Abstract Negative interactions with humans resulting from livestock predation is a major factor influencing the decline of African lion Panthera leo populations across Africa. Here we investigate lion depredation within two Maasai communities in southern Kenya where people and lions coexist in the absence of any formal protected areas. We explore the factors that increase the frequency and severity of lion attacks on pastoralists and their livestock and assess the effectiveness of livestock guarding to reduce damage. Finally, we examine in which circumstances lion depredation triggers retaliation by people. Over a period of 26 months, lions attacked livestock 29 times, resulting in 41 livestock deaths and 19 injuries. There were also two attacks on people. Lions preferred cattle over the more numerous sheep and goats. Attacks on livestock occurred mostly during the dry season and were not affected by changes in prey density or variation in pastoral settlement that brought livestock into closer proximity with lions. Livestock were guarded during 48.2% of lion attacks. Active guarding at pasture disrupted the majority of lion attacks, resulting in lower mortality rates. Passive guarding in corrals at night also disrupted attacks but did not lead to lower livestock mortality.

Maturation and fertilization of African lion (Panthera leo) oocytes after vitrification

The African lion is an excellent model species for the highly endangered Asiatic lion. African lions reproduce well in zoos, leading to the fact that occasionally ovaries and testis are available for in-vitro experiments. We previously performed in-vitro maturation (IVM) and fertilization of lion oocytes and were able to produce advanced embryos after intracytoplasmic sperm injection (ICSI) with cryopreserved sperm. Here we examined whether our in-vitro method is also applicable after vitrification of immature oocytes. Oocytes of four lionesses (5–7 years old) were obtained after euthanasia and immediately processed on site. Half of the oocytes (n = 60) were subjected to IVM for a total of 32–34 h at 39 °C, 5% CO2 and humidified air atmosphere. The second group (59 oocytes) was vitrified instantly using the Cryotop method. Following 6 days of storage in liquid nitrogen, oocytes were warmed and subjected to IVM as well. Mature oocytes of both groups were fertilized with frozen-thawed African lion sperm using ICSI. Maturation rate was 55% and 49.2% for the control and vitrified group, respectively. In the control group, three oocytes cleaved and another three were arrested at the pronuclei stage. Due to the low fertilization result, a sperm sample of another male was used for the vitrified group. Of the vitrified oocytes 7 cleaved and 9 more oocytes stopped at pronuclei stage. All embryos of the vitrified group did not develop beyond 4 cell stage. This is the first time that African lion in-vitro-derived embryos have been produced following oocyte vitrification.

Evaluating the effects of a conservation intervention on rural farmers’ attitudes toward lions

ABSTRACT Using an experimental approach, we analyzed the self-reported changes in farmers’ attitudes toward the African lion (Panthera leo) and a community-based lion conservation program (i.e., the Long Shields Community Guardians), before and after implementation. This program protects livestock and mitigates lion depredation through non-lethal means, with the aim of promoting human-lion coexistence. Results indicated a significant positive shift in attitudes of lions by farmers in the treatment group. This was also the case for farmers in what was recognized as a second unplanned treatment group, called the intermediate group, who learned about the program through communication within their peer network. This article contributed to the growing literature on conservation evaluation and demonstrates that people’s attitudes are valuable for assessing the impact of conservation programs.

Disentangling the roles of bottom‐up and top‐down drivers in the trade‐off between food acquisition and safety in prey with multiple predators

Abstract Prey face a trade‐off between acquiring food and avoiding predation, but food availability, and therefore its effect, is rarely measured in field studies investigating non‐lethal effects of predation. The main aim of this study was to investigate the role of the presence of predators in the functional adjustments of feeding parameters with patch quality in a medium‐sized herbivore. In Hwange National Park (Zimbabwe), we set up an experiment by manipulating, over two years, patch quality for impala Aepyceros melampus, a medium‐sized herbivore. We assess predation risk by monitoring the presence of three GPS‐equipped predators: African lions Panthera leo, spotted hyaenas Crocuta crocuta and African wild dogs Lycaon pictus. In enriched, fertilised plots the impalas reduced step rates (i.e. the rates of change in feeding stations), and increased their number of bites per feeding station while bite rates were not affected. Thus, the main adjustment of their feeding was the step rate. The total time the impalas spent vigilant appeared to be a good predictor of the variation of their bite rate. Although vigilance caused a reduction in bite rate when at a feeding station, the impalas reduced the relative costs of vigilance by continuing chewing and processing their food when scanning for predators. When predators were in the vicinity, the impalas increased their exclusive vigilance (high‐cost vigilance) but not their vigilance while chewing (low‐cost vigilance) and decreased their bite rate while their step rate and the number of bites per feeding station did not change significantly. The impalas were thus visually disconnected from their patch, and reduced their bite rate when actually foraging. Exclusive vigilance increased when both lions and hyaenas were in the vicinity, and when wild dogs were nearby. Patterns of vigilance that altered bite rate were linked to the presence of predators during the previous 24 hr. Over the long term patch quality was the main determinant of the feeding parameters (step rate and bite rate). This study shows how predators, by affecting the time prey devote to predator detection, shape the functional adjustments of food acquisition by prey to local patch quality. A free Plain Language Summary can be found within the Supporting Information of this article.

First-time application of a PCR-based clonality assay in a large cohort of non-domestic felines

Feline lymphoma, one of the most important malignant tumors in domestic cats, is also increasingly diagnosed in non-domestic felines, most notably, African lions (Panthera leo). The gold standard for the diagnosis of lymphoma is histopathological evaluation. As an additional tool, the PCR for antigen receptor gene rearrangement (PARR) has been established. To support the diagnosis on a molecular level, the PCR-based clonality assay is designed to distinguish between reactive and neoplastic lymphocyte populations. In general, PARR primers are used to target complete immunoglobulin heavy chain V-D-J (IGH-VDJ) and T-cell receptor gamma V-J (TRG-VJ) chain gene rearrangements. In this study, we validated the primer sets used in routine diagnostics of domestic cats for the application in non-domestic felines. Clonality testing was used in 41 non-domestic feline species and the results were interpreted in the light of their clinical history and their pathology. In total, clonality could be detected in 8 non-domestic felines (19.4%), including 3 lymphoma cases confirmed by histopathology. These results confirmed the successful application of domestic feline-specific PARR primers in non-domestic feline species. Diagnostic sensitivity and specificity of the clonality assay were 100% and 88%, respectively. Finally, the overall diagnostic accuracy was 89%.

English

There is a lack of consensus among conservationists regarding the association between trophy hunting and wildlife poaching. Anti-hunting groups argue that trophy hunting is against animal welfare and contributes to wildlife population decline so it must be refuted. On the other hand, pro-hunting groups for advocate regulated hunting as an essential tool for supporting habitat protection and reducing crimes. Regulated hunting creates incentives for conservation through direct and indirect methods and reduces wildlife poaching in areas where ecotourism cannot be practically viable. We used fifteen years' trophy hunting and poaching of African elephant (Loxodonta africana), lion (Panthera leo), Cape buffalo (Syncerus caffer), Common Zebra (Equus quagga), hippopotamus (Common Hippotamus amphibious) and Greater kudu (Tragelaphus strepsiceros) from Selous and Rungwa game reserves in Tanzania. The results showed that there is no evidence of influence of regulated hunting on poaching rate for all species with exception of African elephant. Poaching rate of African elephant was found to be higher than the rate of regulated hunting because of limited number of quota set by Convention on International Trade in Endangered Species (CITES) and international restriction of elephant hunting imposed by the International Union for Conservation of Nature (IUCN). The study suggests that the contentions to stop trophy hunting because of an increase in poaching incidents have no empirical justification. Thus, more effort should be on anti-poaching activities ensuring the adherence to legal hunting regulations. Key words: Poaching, hunting, trophy, conservation, biodiversity.