Ending Commercial Lion Farming in South Africa: A Gap Analysis Approach.

Breeding and housing wild animals in captive environments can pose challenges for their welfare. In South Africa, thousands of lions (Panthera leo) are bred and raised at commercial captive breeding facilities, so called ‘lion farms’, for use in tourism, trophy hunting and traditional medicine. To gain a better understanding of the potential welfare challenges faced by lions on farms we reviewed 91 peer-reviewed articles relating to lion welfare, identified via a systematic review of the scientific literature. Across these studies, we identified 170 different terms relating to negative behaviours and physical health afflictions. The majority of these terms were associated with disease and injury (124; 73%), followed by negative behaviours (19; 11%), negative mental experiences (15; 9%), nutritional concerns (7; 4%), and environmental challenges or discomfort arising from the animal's surroundings (5; 3%). Of the 91 articles, 32 (35%) focused on data concerning captive lions. Only two studies focused specifically on data obtained from lion farms in South Africa, whilst the remainder reported on data collected from zoos, wildlife parks, sanctuaries, game reserves and private ownership. Our preliminary review of the scientific literature draws attention to some of the challenges associated with caring for lions in captivity, and outlines the potential significance of these welfare challenges for commercial lion farms. Our data highlight the apparent lack of scientific research involving captive lion welfare generally, particularly data collected at commercial breeding facilities in South Africa and the consequences this could have for the welfare of thousands of lions within the industry.

South Africa has a large captive lion (Panthera leo) sector, but detailed knowledge on the origin of individuals and any potential genetic value to conservation targets is lacking. In 2021, the South African government committed to closing the sector and have since appointed a Ministerial Lion Task Team (2022) to initiate this process. Some have suggested that captive lions could be integrated into wild populations as part of the process but information on the genetic origins and diversity of captive lions is critical if this is to be explored further. Both the Biodiversity Management Plan for lions in South Africa (2015) and a High-Level Ministerial report for the South African government (2021) have called for more information on the genetic composition of captive lions. To determine the probable origin of captive lions in South Africa we summarised existing survey responses from captive facilities (collected 2017–2018) and CITES permit data (issued 1991–2019). Survey data suggest that most lions were sourced from within the South African captive sector. However, many CITES permits were also issued for the import of lions from across Africa and beyond, indicating possible mixed origins within the sector. To evaluate genetic relationships between captive and wild lions in South Africa we standardised existing microsatellite marker data from three laboratories and analysed genotypes of captive lions from 31 properties. A comparison of captive and wild lion genotypes revealed that the genetic composition of captive lions is currently comparable to existing wild South African lions. Captive lions cluster with similar probabilities to three of four regional reference populations of wild lions included in the study and no major signatures of inbreeding were identified. However, captive lions are highly genetically interconnected across properties and represent a smaller effective population size compared to Kruger National Park, the largest population of wild lions in South Africa, suggesting some risk of future inbreeding. There were also signatures of genetic drift which should be investigated further as it will likely compromise any potential conservation genetic value of captive lions in the future. The findings of this study should be considered when planning the fate of individuals within South Africa’s captive lion sector and within the broader context of African lion conservation.

Simple SummaryIn South Africa, thousands of African lions are bred on farms for commercial purposes, such as tourism, trophy hunting, and traditional medicine. Lions on farms often have direct contact with people, such as farm workers and tourists. Such close contact between wild animals and humans creates opportunities for the spread of zoonotic diseases (diseases that can be passed between animals and people). To help understand the health risks associated with lion farms, our study compiled a list of pathogens (bacteria, viruses, parasites, and fungi) known to affect African lions. We reviewed 148 scientific papers and identified a total of 63 pathogens recorded in both wild and captive lions, most of which were parasites (35, 56%), followed by viruses (17, 27%) and bacteria (11, 17%). This included pathogens that can be passed from lions to other animals and to humans. We also found a total of 83 diseases and clinical symptoms associated with these pathogens. Given that pathogens and their associated infectious diseases can cause harm to both animals and public health, we recommend that the lion farming industry in South Africa takes action to prevent and manage potential disease outbreaks. African lions (Panthera leo) are bred in captivity on commercial farms across South Africa and often have close contact with farm staff, tourists, and other industry workers. As transmission of zoonotic diseases occurs through close proximity between wildlife and humans, these commercial captive breeding operations pose a potential risk to thousands of captive lions and to public health. An understanding of pathogens known to affect lions is needed to effectively assess the risk of disease emergence and transmission within the industry. Here, we conduct a systematic search of the academic literature, identifying 148 peer-reviewed studies, to summarize the range of pathogens and parasites known to affect African lions. A total of 63 pathogenic organisms were recorded, belonging to 35 genera across 30 taxonomic families. Over half were parasites (35, 56%), followed by viruses (17, 27%) and bacteria (11, 17%). A number of novel pathogens representing unidentified and undescribed species were also reported. Among the pathogenic inventory are species that can be transmitted from lions to other species, including humans. In addition, 83 clinical symptoms and diseases associated with these pathogens were identified. Given the risks posed by infectious diseases, this research highlights the potential public health risks associated with the captive breeding industry. We recommend that relevant authorities take imminent action to help prevent and manage the risks posed by zoonotic pathogens on lion farms.

The international trade in lion (Panthera leo) products, particularly bone, has increased substantially over the last decade. The Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) has established a zero-export quota for wild-origin lion bones. Whilst the trade of lion bone is permittable from captive-bred South African populations, there is no established method to differentiate between captive and wild-sourced lion derivatives in trade. This study acts as a preliminary investigation, by examining the stable carbon (δ13C) and nitrogen (δ15N) isotope composition of hair from wild and captive lion populations as well as wild prey animals in South Africa, to judge the accuracy and applicability of this method for future bone analysis. Isotopic values for δ15N are found to be significantly enriched in some wild populations, however it is not possible to discriminate between captive and wild populations using δ13C analysis alone. Using the classification algorithm k-Nearest Neighbour, the origin of simulated data was identified with 70% accuracy. When using the model to test the origin of seized samples, 63% were classified as of wild origin. Our study indicates the potential for stable isotope analysis to discriminate between captive and wild populations. Additional study of captive husbandry, and analysis of bone samples from populations of a known origin and feeding regime is recommended to improve the utility of this method for maintaining transparency in trade.

Two of the eight recognized lion subspecies, North African Barbary lion (Panthera leo leo) and South African Cape lion (Panthera leo melanochaita), have become extinct in the wild in the last 150 years. Based on sequences of mitochondrial DNA (mtDNA) control region (HVR1) extracted from museum specimens of four Barbary and one Cape lion, the former was probably a distinct population characterized by an invariable, unique mtDNA haplotype, whilst the latter was likely a part of the extant southern African lion population. Extinction of the Barbary line, which may still be found in ‘‘generic’’ zoo lions, would further erode lion genetic diversity. Therefore, appropriate management of such animals is important for maintaining the overall genetic diversity of the species. The mtDNA haplotype unique to the Barbary lion, in combination with the small size of the HVR1 analyzed (c. 130 bp), makes it possible and cost-effective to identify unlabelled Barbary specimens kept in museums and ‘‘generic’’ captive lions that may carry the Barbary line. An initial study of five samples from the lion collection of the King of Morocco, tested using this method, shows that they are not maternally Barbary.

The African lion Panthera leo is subject to numerous anthropogenic pressures across its natural range. In South Africa, although free ranging populations are increasing, the number of lions in captivity in private commercial facilities far outnumber those in the wild. South Africa’s captive lion industry was reportedly created primarily to generate income and take pressure off wild populations through the supply of captive-bred lions for trophy hunting. However, the industry has become a highly contentious topic under ongoing international scrutiny and debate. Here, we present new information from direct interviews with workers at two closed-access lion facilities located in North West Province, on how some facilities continue to use legal activities, such as captive breeding and hunting, to facilitate their involvement in the illegal international felid bone trade. The sources also report other illegal and unethical activities including animal welfare violations, unsafe conditions for workers, potential shifts to the commercial exploitation of other felid species such as tigers Panthera tigris and incidents involving poaching of captive lions and tigers by non-affiliated actors. Sources described how some facilities use various tools and tactics, such as security cameras, patrols and messaging apps to avoid detection during inspections. If the South African Government is to be successful in meeting its publicly stated goal of ending the captive lion industry, a comprehensive well-managed plan to transition away from current practices is required. To aid enforcement, the industry should also be fully audited, with all facilities officially registered, a moratorium on the breeding of lions and plans put in place to prevent the stockpiling of lion bones.

The lion (Panthera leo) is an iconic resident of zoos and wild animal parks throughout the world. Regular assessment of the morbidity of captive lions is necessary to address wellness concerns and improve the healthcare and management of this vulnerable species. In an effort to understand disease morbidity broadly and guide future inquiries into captive lion health, we distributed a questionnaire that emphasized diseases of organ systems rather than individual diagnoses. We sent the questionnaire to 108 American Zoo and Aquarium Association (AZA) institutions housing lions between 2001 and 2016. Fifty-six facilities responded of which 32 responses had usable data for 111 lions. Responses were compiled and analyzed with respect to age, class, and sex. Neoplasia, renal disease, and dental disease were the most common causes of morbidity in these captive lions. Older animals generally experienced a higher prevalence of multiple types of disease. Three cases of hypertrophic cardiomyopathies (HCM) were reported in nearly 40% (3/8) of cardiovascular diseases and 3% (3/111) of disease cases overall, which is the first report of HCM in lions, to the authors' knowledge. There is a relative paucity of literature on lion and large felid cardiovascular conditions, which suggests that this may be an understudied area of lion health. This broad assessment of morbidities present in captive lion populations aimed to identify key areas for further research and screenings. Management priorities of captive lions should include prevention strategies for dental disease and trauma, frequent screening for renal disease and neoplasia, and further research into cardiovascular health.

Assessing the potential for a levy-based system to replace revenue from trophy hunting in South Africa

Although limited, studies have found conflicting results on whether co-grazing results in significant antibiotic resistance transfer between species. This type of farming system can act as a vector in the geographical spread of antibiotic-resistant bacteria in the environment. The aim of this study was to determine the antibiotic-resistant patterns between co-grazing and non-co-grazing livestock and wildlife species in South Africa. Escherichia coli was isolated from the faeces of various wildlife and livestock species from two farms in South Africa and was tested for antibiotic resistance using the Kirby–Bauer disk diffusion method against chloramphenicol, nalidixic acid, ampicillin, streptomycin, sulphafurazole, and tetracycline. A selection of some common antibiotic-resistant genes (blaCMY, aadA1, sul1, sul2, tetA, and tetB) were detected using PCR. The E. coli isolates from wildlife and livestock that co-grazed showed no significant differences in antibiotic resistance patterns. However, this was not the case for tetracycline resistance as the livestock isolates were significantly more resistant than the co-grazing wildlife isolates. The E. coli isolates from the non-co-grazing livestock and wildlife had significant differences in their antibiotic susceptibility patterns; the wildlife E. coli isolates were significantly more resistant to sulphafurazole and streptomycin than the livestock isolates, whilst those isolated from the cattle were significantly more resistant to ampicillin than the wildlife and sheep isolates. The results of this study suggest that there could be an exchange of antibiotic-resistant bacteria and genes between livestock and wildlife that co-graze.

Effect of age and body condition score on reproductive organ size and sperm parameters in captive male African lion (Panthera leo): Suggesting a prime breeding age

Feline immunodeficiency virus (FIV) is a lentivirus in the Retroviridae family that causes lifelong infection in domestic cats. The lentivirus of African lions (Panthera leo), referred to as FIVple, is endemic in certain lion populations in eastern and southern Africa. Lentivirus infection leads to immunologic dysfunction and immunosuppressive disease in domestic cats; however, little is known about the pathogenic effects of infection in lions, nor about the epidemiologic impact on free-ranging and captive populations. Whole blood and serum samples were collected opportunistically from free-ranging lions in Kruger National Park, Republic of South Africa (RSA). Whole blood and serum samples were also collected from captive wild lions in the RSA. A nested polymerase chain reaction (PCR) assay for detection of FIV was performed on all whole blood samples. In addition, serum samples were tested for cross-reactive antibodies to domestic feline lentivirus antigens and puma lentivirus synthetic envelope peptide antigen. The PCR assay successfully amplified the lion lentivirus from African lions. The relative sensitivity and relative specificity were 79% and 100%, respectively, and the positive and negative predictive values were 100% and 67%, respectively. This research represents the first study to compare genetic material with antibody-based methods of lentivirus detection on lions in RSA. Using PCR as an additional diagnostic test for FIV in lions will increase screening sensitivity and will allow viral characterization among circulating isolates and monitoring of changes in the viral epidemiology within geographic regions and populations over time.

The captive breeding of wildlife for commercial purposes is a controversial issue. In South Africa, the farming of African lions (Panthera leo) for commercial trade emerged in the early 1990s, partly as a conservation measure to reduce the decline in wild lion numbers while meeting increasing wildlife trade demands. In May 2021, the South African Department of Forestry, Fisheries, and the Environment (DFFE) announced plans to end the captive breeding of lions, keeping of lions in captivity, and the use of captive lions, their parts and derivatives for commercial trade. Here, we examined the commercial captive lion industry from 2017 to 2020 in the Free State province, the heart of the lion breeding industry. We document the extent of the industry and highlight a number of key management issues. Of particular concern were issues with microchip numbers, which are used to follow each registered lion from birth to death through the system and to avoid laundering of wild caught and/or non-registered lions. Of the 4,823 unique microchips that were identified, at least 11% could not be followed through the system. Additionally, a minimum of 199 microchip numbers may have been reused by permit holders, either on captivity, euthanasia, or transport permits, indicating potential non-compliance with the Threatened or Protected Species (TOPS) Regulations. We highlight further areas of concern that warrant additional attention for these types of activities that may cause management issues during the transition period and which may also be relevant for the keeping, breeding, and trading of other TOPS regulated species in South Africa, particularly big cat species.

The economy, unemployment, and job creation of South Africa heavily depend on the growth of the agricultural sector. With a growing population of 60 million, there are approximately 4 million small-scale farmers (SSF) number, and about 36,000 commercial farmers which serve South Africa. The agricultural sector in South Africa faces challenges such as climate change, lack of access to infrastructure and training, high labour costs, limited access to modern technology, and resource constraints. Precision agriculture (PA) using AI can address many of these issues for small-scale farmers by improving access to technology, reducing production costs, enhancing skills and training, improving data management, and providing better irrigation infrastructure and transport access. However, there is a dearth of research on the application of precision agriculture using artificial intelligence (AI) by small scale farmers (SSF) in South Africa and Africa at large. The preferred reporting items for systematic reviews and meta-analyses (PRISMA) and Bibliometric analysis guidelines were used to investigate the adoption of precision agriculture and its socio-economic implications for small-scale farmers in South Africa or the systematic literature review (SLR) compared various challenges and the use of PA and AI for small-scale farmers. The incorporation of AI-driven PA offers a significant increase in productivity and efficiency. Through a detailed systematic review of existing literature from inception to date, this study examines 182 articles synthesized from two major databases (Scopus and Web of Science). The systematic review was conducted using the machine learning tool R Studio. The study analyzed the literature review articled identified, challenges, and potential societal impact of AI-driven precision agriculture.

Interspecies comparison of the residue levels and profiles of persistent organic pollutants in terrestrial top predators

A mixed infection of Notodres cati and Ancylostoma caninum was recorded among captive lions ( Panthera leo ) at the Sanda Kyarimi Park in Maiduguri, Nigeria. All of the 7 lions (3 adults and 4 cubs) examined in the Park were infested with the mite, Notodres cati. While, 5 (71.4%) out of the 7 examined were shedding A. caninum ova in their faeces. Faecal egg counts were significantly higher (p Keywords : Mixed infection, lions, Sanda Kyarimi Park, Maiduguri, Nigeria Sahel Journal of Veterinary Sciences Vol. 5 (1) 2006 pp. 5-8