Aflatoxins In Foods Research Articles

Minimal interference of glucose and ethanol on aflatoxin B1 adsorption by smectites

Due to stringent regulations on aflatoxins in food and feed, and an increased demand for biofuel, it is reasonable to direct aflatoxin-contaminated corn to biofuel production. Of great concern is the up to three-fold mycotoxin enrichment in distiller's grains, a co-product of ethanol biofuel production. The objective of this study was to test the interferences of ethanol and glucose on aflatoxin adsorption by smectites. Ethanol and glucose are two of the major soluble compounds present in fermentation solutions. The aflatoxin adsorption capacities of four smectites from Mississippi (2MS, 3MS, and 4MS) and Alabama (MBBO1) were evaluated in water and in ethanol/glucose. The highest aflatoxin B1 adsorption capacity (Qmax) of the four smectites in water was 0.59molkg−1 (184gkg−1). Batch adsorption experiments suggested that smectites effectively adsorbed aflatoxin B1 from the simulated fermentation solutions. Aflatoxin adsorption from 10% (w/v) glucose and 20% (v/v) ethanol to clay was 90% of aflatoxin adsorption to clay from pure distilled water. X-ray diffraction analysis indicated that aflatoxin–smectite complexes formed in water, 10% glucose, and 20% ethanol solutions responded similarly to heat treatment and maintained basal spacings >1.2nm up to 300°C, whereas the smectite without aflatoxin treatment collapsed to 1.0nm at 150°C. The greater basal spacing after heat treatment suggested that smectite interlayers were accessible to aflatoxin in the presence of high glucose and ethanol concentrations. Infrared spectra of aflatoxin–smectite complexes formed in the simulated fermentation solutions were nearly identical to infrared spectra of aflatoxin–smectite complexes formed in distilled water. This suggests that ethanol and glucose had little influence on the type or strength of bonding between aflatoxin and smectite and that washing with water easily removed any adsorbed ethanol and glucose. The distinct two-carbonyl bond in-phase stretching vibration at 1727cm−1 revealed irreversible aflatoxin B1 bonding in smectite interlayers. It can be concluded that glucose and ethanol interferences with aflatoxin adsorption to smectites is minimal.

Methods for Detection of Aflatoxins in Agricultural Food Crops

Aflatoxins are toxic carcinogenic secondary metabolites produced predominantly by two fungal species: Aspergillus flavus and Aspergillus parasiticus. These fungal species are contaminants of foodstuff as well as feeds and are responsible for aflatoxin contamination of these agro products. The toxicity and potency of aflatoxins make them the primary health hazard as well as responsible for losses associated with contaminations of processed foods and feeds. Determination of aflatoxins concentration in food stuff and feeds is thus very important. However, due to their low concentration in foods and feedstuff, analytical methods for detection and quantification of aflatoxins have to be specific, sensitive, and simple to carry out. Several methods including thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), mass spectroscopy, enzyme-linked immune-sorbent assay (ELISA), and electrochemical immunosensor, among others, have been described for detecting and quantifying aflatoxins in foods. Each of these methods has advantages and limitations in aflatoxins analysis. This review critically examines each of the methods used for detection of aflatoxins in foodstuff, highlighting the advantages and limitations of each method. Finally, a way forward for overcoming such obstacles is suggested.

Determination of Aflatoxins in Sesame, Rice, Millet and Acha from Nigeria using HPLC

A hundred and twenty (120) Nigerian food commodities including; rice (15), madidi (rice product) (15), millet (15), fura (millet dough) (15), sesame (30) and fonio (30) were collected, subjected to aflatoxin extraction and clean up procedures and analyzed quantitatively for aflatoxins using High performance liquid chromatography. Five of the rice samples contained aflatoxin B1 within the range of 37.26-113.2 µg/kg, for madidi samples, 7 contained AFB1 within the range of 0.20-112.5µg/kg, 3 contained AFB2 within the range of 0.95-18.40 µg/kg while 6.88 µg/kg and 1.60 µg/kg of the green fluorescing aflatoxins 1 and 2 respectively was found in one sample each. Analysis of sesame samples showed that eight contained AFB1 within the range of 14.71-140.9 µg/kg, while one sample contained 2.61 µg/kg of aflatoxin G1. The millet, millet dough and fonio samples all tested negative for the presence of aflatoxins indicating some degree of resistance. All contaminated rice and sesame samples were at ranges above the EU and Nigerian legislated limits for AFB1 and aflatoxins in food. This portrays associated health consequence to consumers and negative impact on trade of these commodities both locally and internationally.

Global health issues of aflatoxins in food and agriculture: challenges and opportunities.

Global health issues of aflatoxins in food and agriculture: challenges and opportunities.

Fluorescence Excitation–Emission Features of Aflatoxin and Related Secondary Metabolites and Their Application for Rapid Detection of Mycotoxins

The persistent occurrence of aflatoxins in food and feed remains a problem for producers of commodities subject to colonization with toxigenic molds. Aflatoxins are secondary metabolites of fungi of the Aspergillus spp. associated with deleterious health effects. Because current screening methods for these toxins are lengthy, destructive, and costly, there is a continuous search for a more rapid, noninvasive, and cost-effective technology. The present study utilized a fluorescence excitation–emission matrix (EEM) of aflatoxin as well as two additional secondary metabolites (kojic acid and the bright greenish-yellow fluorescence (BGYF) compound) of Aspergillus flavus measured with a fluorescence spectrophotometer. The results were compared to image data acquired with a fluorescence hyperspectral sensor in order to evaluate the potential of image-based technology for detecting aflatoxin in grain. The excitation–emission matrix of aflatoxin B1 standard produced overlapping peaks in 340–400 nm of excitation range emitting in the blue range at around 450 nm. The spectral signature extracted from the hyperspectral image was also in the blue range, emitting blue fluorescence. Because the results from both systems were comparable, where all fluorescence peaks were in the blue range, the present study validates the feasibility of image-based technology for nondestructive detection of aflatoxin in corn. Additional peaks were revealed in the aflatoxin EEM in the 260-nm excitation range that were not present in the kojic acid and BGYF compound mixture. This new information allows for the separation of the aflatoxin signature from the potentially confounding overlap of other secondary metabolites occurring in the blue and blue-green spectral ranges.

Occurrence of toxigenic Aspergillus spp. and aflatoxins in selected food commodities of Asian origin sourced in the West of Scotland

The occurrence of Aspergillus moulds and aflatoxins in 12 commercially-available dried foods of Asian origin were examined. All food samples, except green beans and three types of dried fruit, contained multiple genera of moulds of which Aspergillus (55%) was the most frequently detected. Penicillium (15%), Rhizopus (11%), Mucor (3%), Monascus (1%), Eurotium (1%) and unidentified (14%) were also observed. The occurrence of aflatoxigenic moulds, however, did not correspond with the occurrence of aflatoxins in foods. Aflatoxigenic Aspergillus spp. (39 isolates) were recovered from long grain rice, fragrant rice, peanuts, black beans and black pepper. The predominant Aspergillus species was A. parasiticus (61%) while Aspergillus oryzae (3%), Aspergillus utus (5%), Aspergillus niger (5%), Aspergillus ochraceus (3%) and unidentified (23%) were also observed. Long grain rice, fragrant rice, peanuts, black beans and black pepper were positive for Aspergillus but contained undetectable aflatoxins. In contrast, Jasmine brown rice and crushed chilli contained 14.7 and 11.4μg/kg of total aflatoxins, respectively, in the absence of Aspergillus so aflatoxigenic Aspergillus was present at some stage of food production. The results from this study emphasise the need for stricter control measures in reducing occurrence of aflatoxins in foods for export and domestic use.

Aflatoxin M1 contamination of human breast milk in Isfahan, Iran.

Background:During the last decades there has been great attention paid to aflatoxins. They are highly toxic, immunosuppressive, mutagenic, teratogenic, and carcinogenic compounds. Aflatoxin M1 (AFM1), a hydroxylated metabolite of aflatoxin B1 (AFB1), is formed in the liver and excreted into the breast milk. It is considered to cause certain hygienic risks for infant health. The aim of this study was to evaluate the presence of the AFM1 in the breast milk using AFM1 in milk as a biomarker for exposure to aflatoxin B1 and determine the level of AFM1 contamination in the lactating mothers in Isfahan, Iran.Materials and Methods:This study was carried out on 80 lactating women randomly selected from two urban health centers. Mother's milk samples and information on food intake were collected from the participants using structured food-frequency questionnaire. Breast milk samples were tested for AFM1 by a competitive ELISA technique.Results:Our findings showed that only one sample was contaminated with AFM1 with concentrations of 6.8 ng/L. However, the AFM1 level in this sample was lower than the maximum tolerable limit (25 ng/L) accepted by the European Communities and Codex Alimentarius.Conclusion:Although the concentration of AFM1 in none of the samples was higher than the acceptable level, the presence of AFM1 in only one of them confirms the need for developing strategies to reduce exposure to aflatoxin in foods and to carry out biological monitoring of aflatoxins as a food quality control measure routinely.

Validation of immunoenzymatic tests for the detection of aflatoxin present in food

The significance of this study is reflected in proving the reliability of the ELISA test for the detection of aflatoxin in food and feed. Verification tests were based on the use of reference materials with known concentrations of aflatoxins, contamination of samples by adding known concentrations of analyte and participation in inter-laboratory studies, by different perpetrators and different laboratories. By measuring the known con?centrations of analytes it was proved that aflatoxins, in the selected samples, were determined in accordance with the requirements defined for a given method, and it was demonstrated that it is possible to determine the level / concentration of mycotoxins in relation to the MRL defined by the Regulation on amendments to the Ordinance on the maximum allowable residue levels of pesticides in food and feed and feed and feed for which maximum allowed quantities of residues of plant protection products (Sluzbeni Glasnik RS broj 28/2011 clan 2. prilog 5. tacka 2).

Effects of feeding corn naturally contaminated with AFB<sub>1</sub> and AFB<sub>2</sub> on performance and aflatoxin residues in broilers

 AFB<sub>1</sub> is the most abundant aflatoxin in food and animal feed, generally occurring along with low levels of other aflatoxins. In this experiment, broilers were administered corn that was naturally contaminated with AFB<sub>1</sub> and AFB<sub>2</sub>. We found that the broilers were more sensitive during the starter period and had a poor performance during the grower period. In addition, the broilers showed a significant reduction in feed intake when consuming material contaminated with 134.0 and 23.6 µg/kg of AFB<sub>1</sub> and AFB<sub>2</sub>, respectively. Our results also demonstrate that villus height and the ratio of villus height to crypt depth significantly decreased when the broilers were fed daily with diets contaminated with AFB<sub>1</sub> and AFB<sub>2</sub>. Furthermore, AFB<sub>1</sub> residues in livers and breast muscles were determined by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) at levels of 0.137 and 0.016 µg/kg, respectively. The AFM<sub>1</sub> residue was also detected in livers at a level of 0.051 µg/kg but it was not detectable in breast muscles.

Determination of aflatoxins in food using liquid chromatography coupled with electrospray ionization quadrupole time of flight mass spectrometry (LC-ESI-QTOF-MS/MS)

A rapid, reliable and confirmatory method has been developed to determine the levels of aflatoxins B1, B2, G1, and G2 in barley, wheat, soybean, corn, peanut and peanut butter. The method is based on a single extraction step followed by liquid chromatography coupled with electrospray ionization quadrupole time of flight mass spectrometry (LC-ESI-QTOF-MS/MS). The extraction, dilution, chromatographic and MS conditions were optimized in order to increase the sample throughput and sensitivity. The quantification of the analytes was measured by performing low-energy collision induced tandem mass spectrometry (CID-MS/MS) using the product ion scan mode. The calibration curves were linear over the range from 0.195 to 50.000 μg/kg for aflatoxin B1, G1 and from 0.029 to 15.000 μg/kg for aflatoxin B2, G2. The correlation coefficients (r2) were higher than 0.998. The overall recoveries for aflatoxins were in the range of 57.1–108.7% at spike levels of 0.6–15.0 μg/kg with relative standard deviations lower than 16%. The limit of quantification (LOQ) for aflatoxins is less than 0.8 μg/kg.

Simultaneous Determination of Aflatoxin B1 and Aflatoxin M1 in Food Matrices by Enzyme-Linked Immunosorbent Assay

Aflatoxins are a class of highly toxic chemical contaminants occurring in food. Consumption of aflatoxin-contaminated food can lead to harmful effects on human health. A rapid and reliable analysis of aflatoxins in food is crucial. In this study, we generated a broad–specific monoclonal antibody (MAb) 3 C10 against aflatoxin B1 (AFB1). The MAb 3 C10 binds specifically to AFB1 and AFM1 and has a IC50 of 0.13 μg L−1 for AFB1 and 0.16 μg L−1 for AFM1. Furthermore, the MAb showed high cross-reactivity to AFB2, AFG1, and AFG2. To enable simultaneous AFB1 and AFM1 detection in different food matrices, an indirect competitive enzyme-linked immunosorbent assay (icELISA) based on MAb 3 C10 has been developed and optimized. In addition, the extraction methods of different food matrices (peanut, corn, soybean, wheat flour, rice, soy sauce, vinegar, wine, raw milk, pure milk, skimmed milk, and yogurt) were established. The average recovery ranged from 73 to 121 %, with relative standard deviation values less than 15 %. The limit of detection was 0.52 + 0.36 μg kg−1 (mean + 3SD) for AFB1 in eight agricultural products and 0.031 + 0.015 μg kg−1 (mean + 3SD) for AFM1 in four dairy products. The sensitivity of icELISA was below the limit set by the European Commission for aflatoxin detection in different food matrices and similar to LC–MS/MS method. We demonstrate a rapid, simple, and reliable method for simultaneous screening of AFB1 and AFM1 in different food matrices.

Determination of Mould and Aflatoxin Contamination in Tarhana, a Turkish Fermented Food

Tarhana is a popular traditional Turkish cereal-based fermented food product mainly produced at home or at home-scale level. Some certain mould species can grow even at low moisture and pH values and produce aflatoxins in food. This study was conducted to determine aflatoksin levels in tarhana. For this purpose, a total of 138 tarhana powder samples were collected from bazaars in Istanbul and analyzed for aflatoxins, mould contamination, and some physco-chemical parameters. As a result, 32 out of 138 tarhana samples (23.2%) were found to be contaminated with aflatoxins in the range of 0.7–16.8 μg/kg, whereas 29 samples contained Aflatoxin B1 (AFB1) ranging from 0.2–13.2 μg/kg. All samples (100%) contaminated with moulds in the range of 1.4 × 101 –5.8 × 107cfu/g. The average pH, moisture and aw results were detected as 3.82, 12.71%, and 0.695, respectively.

The Prevalence of Aflatoxinogenic Aspergillus parasiticus in Jordan.

Aflatoxins are potent carcinogens and produced by almost all Aspergillus parasiticus isolates and about 35% of Aspergillus flavus isolates. Chemical methods are used for detection of aflatoxins in food and feed. These methods cannot detect aflatoxinogenic fungi in samples, which contain undetectable amounts of aflatoxins. The objective of this research work was to ascertain the importance of molecular and microbiological methods in detection of aflatoxinogenic fungus A. parasiticus in food and feed samples in Jordan. Specific media for the detection of aflatoxins showed the prevalence of A. parasiticus (6–22%) in contaminated food and feed samples. HPLC method confirmed the presence of aflatoxins B1, B2, G1, and G2 in food sample contaminated with A. parasiticus. Primer set OmtBII-F and OmtBII-R amplified DNA fragment of 611 base pairs from genomic DNA of aflatoxinogenic A. parasiticus isolated from food and feed samples but could not amplify DNA fragment of nonaflatoxinogenic A. flavus. The results of this study showed the prevalence of aflatoxinogenic A. parasiticus in food and feed samples in Jordan and give further evidence of suitability of microbiological and molecular methods in detection of aflatoxins, which are reliable low-cost approach to determine food and feed biosafety.

A fungal enzyme with the ability of aflatoxin B 1 conversion: Purification and ESI-MS/MS identification

Armillariella tabescens, a Chinese edible and medicinal fungus, whose multienzyme exist ability of AFB 1-converting, and ADTZ (aflatoxin-detoxizyme) had previously purified from the A. tabescens multienzyme monitored by AFB 1 conversion . However, the enzyme now confirmed an oxidase and renamed aflatoxin-oxidase (AFO). In this paper, AFO was purified by an economical and practical three-step procedure monitored by AFB 1 conversion. And ESI-MS/MS analysis was done for identification of AFO. The following database searching (Protein Blast on NCBI) results did not show any homologous oxidase protein, which implied that AFO was mostly a new oxidase differing from other reported aflatoxin-converting enzymes such as fungal laccase and horse radish peroxidase. HPTLC analysis of the purified AFO activity suggested that the enzyme reacted at the bisfuran ring of AFB 1 which was the key toxic structure. Therefore, all these investigations implied a new choice for biodegradation of aflatoxin in foods and feeds with the practical application of AFO.

Microbial strategies to control aflatoxins in food and feed

Aflatoxins are a group of toxic and carcinogenic fungal metabolites. They are commonly found in cereals, nuts and animal feeds and create a significant threat to the food industry and animal production. Several strategies have been developed to avoid or reduce harmful effects of aflatoxins since the 1960s. However, prevention of aflatoxin contamination pre/post harvest or during storage has not been satisfactory and control strategies such as physical removing and chemical inactivating used in food commodities have their deficiencies, which limit their large scale application. It is expected that progress in the control of aflatoxin contamination will depend on the introduction of technologies for specific, efficient and environmentally sound detoxification. The utilisation of biological detoxification agents, such as microorganisms and/or their enzymatic products to detoxify aflatoxins in contaminated food and feed can be a choice of such technology. To date, many of the microbial strategies have only showed reduced concentration of aflatoxins and the structure and toxicity of the detoxified products are unclear. More attention should be paid to the detoxification reactions, the structure of biotransformed products and the enzymes responsible for the detoxification. In this article, microbial strategies for aflatoxin control such as microbial binding and microbial biotransformation are reviewed.

A neutral red desiccated coconut agar for rapid detection of aflatoxigenic fungi and visual determination of aflatoxins

Desiccated coconut agar is the conventional medium used for the detection of aflatoxigenic fungi and direct visual determination of aflatoxins. In this study, an improved medium was developed by the incorporation of 0.2% (v/v) neutral red dye into desiccated coconut agar. The medium was formulated by a 2×3 factorial design of neutral red and phenol red stains at three concentration levels. The formulated medium was evaluated for performance by screening for the minimal time required by each Aspergillus species to produce pigments and fluorescence of agar. The medium was also employed for detection of aflatoxigenic fungi and direct visual determination of aflatoxins in foods and fish-meal. The neutral red desiccated coconut agar (NRDCA) as compared to the conventional desiccated coconut agar (DCA) had a light pink background as opposed to the white background of the DCA which often interferes with the visibility of fluorescence. The time of pigmentation and fluorescence production on NRDCA was 28 and 38 h respectively as compared with 33 and 44 h of DCA and 41 and 48 h of palm kernel agar (PKA: an alternative culture medium for cultivation of aflatoxigenic fungi with a reddish pink background). Furthermore, aflatoxigenic moulds were detected in all food commodities and fish-meal after 60 hours of incubation. The highest percentage of aflatoxigenic moulds (62.5%) was detected in yam flour with NRDCA while the lowest percentage (4.46%) was detected with PKA on rice. In addition, aflatoxins were produced in high amounts in food commodities in which aflatoxigenic moulds were detected and there was a significant positive correlation (r=0.4, P<0.05) between the isolates and aflatoxin concentration of the food samples. Rice (a major staple food for Nigerians) had the highest total aflatoxin concentration of 140, 220 and 205 µg/kg on DCA, NRDCA and PKA respectively, while ‘gari’ had the least concentration of 45, 50 and 40 µg/kg. These values were far above the NAFDAC recommended level of 10 µg/kg for unprocessed foods in Nigeria and therefore a source of concern. In addition the study also reveals that Aspergillus nomius can produce aflatoxins B1 in copious amounts on NRDCA, contrary to previous reports of its production in minute quantities on laboratory media. The benefit of this study lies in the rapid analysis and simplified technique for the detection of aflatoxigenic fungi and visual determination of aflatoxins.

Costs and efficacy of public health interventions to reduce aflatoxin-induced human disease

This study reviews available information on the economics and efficacy of aflatoxin risk-reduction interventions, and it provides an approach for analysis of the cost-effectiveness of public health interventions to reduce aflatoxin-induced human disease. Many strategies have been developed to reduce aflatoxin or its adverse effects in the body. However, a question that has been under-addressed is how likely these strategies will be adopted in the countries that need them most to improve public health. This study evaluates two aspects crucial to the adoption of new technologies and methods: the costs and the efficacy of different strategies. First, different aflatoxin risk-reduction strategies are described and categorized into pre-harvest, post-harvest, dietary, and clinical settings. Relevant data on the costs and efficacy of each strategy, in reducing either aflatoxin in food or its metabolites in the body are then compiled and discussed. In addition, we describe which crops are affected by each intervention, who is likely to pay for the control strategy, and who is likely to benefit. A framework is described for how to evaluate cost-effectiveness of strategies according to World Health Organization (WHO) standards. Finally, it is discussed which strategies are likely to be cost-effective and helpful under different conditions worldwide of regulations, local produce and soil ecology, and potential health emergencies.

Isolation and screening of biocontrol bacterial strains against Aspergillus parasiticus from groundnut geocarposphere

INTRODUCTION Aspergillus parasiticus Speare and A. flavus Link are two major fungi species that produce aflatoxins in agricultural commodities, such as groundnut, corn and cottonseeds. Because of the potency of aflatoxins as carcinogens and the associated food and feed safety hazards, worldwide regulatory limits have been established for aflatoxins in food and animal feeds (van Egmond, 1995). Groundnut (Arachis hypogaea L.) is a major oilseed crop widely grown in tropical and subtropical regions of the world. Aflatoxin contamination of groundnut by A. flavus and A. parasiticus is a serious problem and has a tremendous impact on the global groundnut industry as well as posing public health risks (Anjaiah et al., 2006). Therefore, control of aflatoxin contamination is very crucial for food and feed safety. Bacteria have been investigated for antifungal and aflatoxin-inhibiting activity against A. flavus and

ELISA-LC/MS/MS 병행에 의한 식품 중 aflatoxins 분석

High performance liquid chromatography (HPLC) and liquid chromatography mass spectrometry (LC/MS) have been widely used to quantify aflatoxins in food, but these methods are expensive, time-consuming, unsuitable for analysis of the routine screening of large sample numbers and require derivatization and high level techniques to perform. The objective of this study is to detect aflatoxins in a large number of foods by a high efficient analytical system of combined enzyme linked immunosorbent assay (ELISA) for screening and LC/MS/MS for confirmation. The samples spiked individually with aflatoxin <TEX>$B_1$</TEX> (0.5 and 1.0 ng/g) and total aflatoxins (10 ng/g) were analyzed by ELISA and LC/MS/ MS, and the recoveries for ELISA and LC/MS/MS were 71.8~119.2% and 70.8~135.3%, respectively. A total of 378 samples (grains, nuts, soybean and fermented soybean foods, pepper and fermented pepper foods) were purchased from the six major cities in Korea and analyzed by ELISA-LC/MS/MS system. Twenty two (5.8%; peanut: 11, pistachio: 2, walnut: 6, almond: 1, pepper powder: 1, pepper paste: 1) out of 378 samples were screened as aflatoxin B1 positive by ELISA, but, 4 (1.1%; peanut: 2, pistachio:1, pepper powder: 1) out of the 22 samples screened were confirmed as aflatoxins positive at levels of 1.02~52.79 ng/g by LC/MS/MS. ELISA-LC/MS/MS system provides a more rapid, accurate and cost-effective method for the detection of aflatoxins in large number of samples.

Development of low aflatoxin soycorn milk: optimisation of soybean and sweet corn ratio and its stability during storage

A nutritious soycorn milk can be produced by combining soybean and corn. The beverage offers dual nutritional advantages for public health in combating protein and Vitamin A deficiency. However, many studies have shown high level aflatoxin contamination in corn and corn foods. The high content of aflatoxin in food become a public health problem throughout the world. In this research, soybean and sweet corn blend have been used to produce low level aflatoxin soycorn milk. Ratio of soybean and sweet corn affected the physicochemical and sensory properties of the soycorn milk. Ratio of soybean and sweet corn of 70:30 produced soycorn milk with the highest consumer acceptance, total protein of 1.12%, total solid of 11.5%, viscosity of 179.10 cP and colour of 0.60 Orange/4.63 Yellow. Aflatoxin B1 was detected in soycorn milk at a low level of 0.24 ng/ml, while aflatoxin B2, G1 and G2 were not detected by High Performance Liquid Chromatography (HPLC). The aflatoxin content in the soycorn milk was below the maximum limit regulated by European Union and USA Food and Drug Administration. Based on the tolerable daily intake set by WHO, it could be calculated the maximum daily consumption of the soycorn milk to control the aflatoxin exposure concerning public health. Further researches to produce soycorn milk with lower content of aflatoxin, even no aflatoxin, are needed. Bacteria growth was found in the soycorn milk during cold storage in refrigerator, with containing bacteria of 4.07 × 102 cfu/ml after three days. Slightly declining of colloidal stability was found during storage. Efforts to extend the shelf life of the soycorn milk are also needed.