Crustaceans detect chemical stimuli in the environment with aesthetasc sensilla, which are located on their 1st antennae. With the transition to other environments, chemoreception faces physical challenges. To provide a deeper understanding of the relation between the morphology of olfactory organs and different lifestyles, we studied the peripheral olfactory system of three amphipod species, the marine Gammarus salinus, the blind subterranean freshwater species Niphargus puteanus, and the terrestrial Cryptorchestia garbinii. We compared the 1st and 2nd antennae of these species with respect to length and presence of aesthetascs and other sensilla. The females of N. puteanus reveal the longest 1st antennae in relation to body size. G. salinus shows the largest aesthetascs and the same relative length of the 1st antennae as male N. puteanus. C. garbinii has very short 1st antennae and reduced (putative) aesthetascs. Our findings show that the compensation of vision loss by olfaction cannot be generally assumed in animals from dark environments. Furthermore, the behaviour of C. garbinii indicates a chemosensory ability, despite the reduction of the 1st antennae. A comparison with other terrestrial crustaceans suggests that the loss of the olfactory sense on the 1st antennae in C. garbinii might be compensated with chemoreception by the 2nd antennae.