Aerobic Methane Oxidation Research Articles

Soil organic matter and salinity as critical factors affecting the bacterial community and function of Phragmites australis dominated riparian and coastal wetlands

Soil salinization poses a great threat to the natural ecosystem and interferes with the structure and function of the biological community, resulting in different vegetation distributions. However, little attention is paid to the changes in microbial community in different wetland types with the same vegetation. In this study, the Yellow River Delta was used as a model because of its typical and extensive distribution of Phragmites australis-dominated saltwater and freshwater wetlands. We investigated the differences in the structure and function of bacterial communities, as well as their relationships with soil properties in coastal (Zone A) and riparian (Zone B) wetlands. Results showed higher salinity and pH in Zone A than Zone B (p < 0.05), whereas TN (p < 0.05) and SOM were lower than those in Zone B. Significant differences existed in microbial community composition between Zones A and B. The nitrifying-bacteria Nitrospira and norank_f_Nitrosomonadaceae had high abundance in Zones A and B. Alcanivorax, Halomonas, and Marinobacter were extensively distributed in Zone A, whereas Flavobacterium and Pseudomonas were dominant in Zone B, indicating the diversity characteristics of denitrifying bacteria. Conversely, methane-oxidizing bacteria Methylophaga were significantly higher in Zone A than in Zone B (p < 0.05), indicating that high salinity was conducive to aerobic methane oxidation and that the genetic diversity at strain level endowed it with a certain denitrification potential. Salinity and SOM played important roles in shaping microbial community at phylum and genus levels. The gene abundances related to xenobiotics metabolism and repair were high in Zone A, whereas the genes encoding energy metabolism and signal transduction were relatively high in Zone B. Denitrification was more favored for the low-salinity Zone B, whereas methane oxidation was enriched in the high-salinity Zone A. Therefore, our study emphasized the importance of an in-depth understanding of the microbial-community structure and function in Phragmites australis-dominated saltwater and freshwater wetlands.

Stable-isotopic and metagenomic analyses reveal metabolic and microbial link of aerobic methane oxidation coupled to denitrification at different O2 levels

Aerobic methane (CH4) oxidation coupled to denitrification (AME-D) can not only mitigate CH4 emission into the atmosphere, but also potentially alleviate nitrogen pollution in surface waters and engineered ecosystems, and it has attracted substantial research interest. O2 concentration plays a key role in AME-D, yet little is understood about how it impacts microbial interactions. Here, we applied isotopically labeled K15NO3 and 13CH4 and metagenomic analyses to investigate the metabolic and microbial link of AME-D at different O2 levels. Among the four experimental O2 levels of 21%,10%, 5% and 2.5% and a CH4 concentration of 8% (i.e., the O2/CH4 ratios of 2.62, 1.26, 0.63 and 0.31), the highest NO3−-N removal occurred in the AME-D system incubated at the O2 concentration of 10%. Methanol and acetate may serve as the trophic linkage between aerobic methanotrophs and denitrifers in the AME-D systems. Methylotrophs including Methylophilus, Methylovorus, Methyloversatilis and Methylotenera were abundant under the O2-sufficient condition with the O2 concentration of 21%, while denitrifiers such as Azoarcus, Thauera and Thiobacillus dominated in the O2-limited environment with the O2 concentration of 10%. The competition of denitrifiers and methylotrophs in the AME-D system for CH4-derived carbon, such as methanol and acetate, might be influenced by chemotactic responses. More methane-derived carbon flowed into methylotrophs under the O2-sufficient condition, while more methane-derived carbon was used for denitrification in the O2-limited environment. These findings can aid in evaluating the distribution and contribution of AME-D and in developing strategies for mitigating CH4 emission and nitrogen pollution in natural and engineered ecosystems.

NC10 bacteria promoted methane oxidation coupled to chlorate reduction.

The strictly anaerobic serum bottles were applied to investigate methane oxidation coupled to chlorate (ClO3-) reduction (MO-CR) without exogenous oxygen. 0.35mM ClO3- was consumed within 20days at the reduction rate of 17.50μM/d, over three times than that of ClO4-. Chlorite (ClO2-) was not detected throughout the experiment and the mass recovery of Cl- was over 89%. Isotope tracing results showed most of 13CH4 was oxided to CO2, and the electrons recovery reached to 77.6%. Small amounts of 13CH4 was consumed for DOC production probably through aerobic methane oxidation process, with oxygen generated from disproportionation reaction. In pMMO (key enzyme in aerobic oxidation of methane) inhibition tests, ClO3- reduction rate was slowed to 7. 0μmol/d by 2mM C2H2, real-time quantitative PCR also showed the transcript abundance of pMMO and Cld were significantly dropped at the later period of experiment, indicating that the O2 disproportionated from ClO2- was utilized to active CH4. NC10 bacteria Candidatus Methylomirabilis, related closely to oxygenic denitrifiers M. oxyfera, was detected in the system, and got enriched along with chlorate reduction. Several pieces of evidence supported that NC10 bacteria promoted CH4 oxidation coupled to ClO3- reduction, these oxygenic denitrifiers may perform ClO2- disproportionation to produce O2, and then oxidized methane intracellularly.

The diagenetic origin and depositional history of the Cherry Valley Member, Middle Devonian Marcellus Formation

We report δ13C, δ18O, δ34S records coupled with elemental, pyrite morphology, and fluid inclusion data spanning the Cherry Valley Member in the Middle Devonian Marcellus shale from two cores located across a depth gradient in the Appalachian basin. The goal of the study was to evaluate the diagenetic and redox history of this carbonate-interbedded layer within a prominent shale gas system. Three lithological units were identified in the Cherry Valley Member: a nodular clay-rich marlstone lower unit, a fossiliferous limestone middle unit, and a calcareous mudstone upper unit. Low δ13C and δ18O values of the carbonate matrix display a mixing trend among marine carbonate, authigenic carbonate, and late stage diagenetic cementation. The petrology of barite nodules and associated δ34Sbarite values suggest that a shoaling sulfate-methane transition zone created by methane advection contributed to the nodule growth and pyritization. Along with other early and late diagenetic processes, this mechanism provided the main carbonate source for the lower unit due to elevated rates of aerobic methane oxidation. The δ34Spyrite values, U and Mo contents, and pyrite framboid size distributions together constrain the redox history of the Cherry Valley Member. An anoxic-euxinic marine environment rather than a fully oxygenated environment continued through the lower unit in areas with deeper paleo-bathymetry until it was interrupted by an episode of dysoxia during the deposition of the middle unit. Basin-scale anoxia was then renewed during a subsequent 3rd order sea level rise in the upper unit. The entire Cherry Valley Member was ultimately altered by warm burial fluid interaction through vertically distributed veins. The combined body of evidence indicates that the duration and spatial distribution of oxygenated marine conditions during the deposition of Cherry Valley Member within the Marcellus Formation may have been overestimated.

Development of aerobic methane oxidation, denitrification coupled to methanogenesis (AMODM) in a microaerophilic expanded granular sludge blanket biofilm reactor

The issue of enhancing nitrogen removal and managing dissolved methane emission in anaerobic treatment systems is a major bottleneck in its wider application to treat high-strength organic wastewater with nitrate. Herein, a novel aerobic methane oxidation, denitrification coupled to methanogenesis (AMODM) process was developed in a glucose-fed microaerobic expanded granular sludge blanket biofilm reactor (EGSBBR) through in-situ utilization of produced methane for nitrogen removal. The 162-day operation demonstrated that long-term treatment performance under the decreased COD/NO3−-N (C/N) ratio from 66.7 to 10 and the optimal C/N ratio for completing AMODM was found to be 16.7. Microbial community analysis further evidenced that Methanothrix as key methanogen predominated in the sludge bed, while Methlogaea as aerobic methane oxidizer was mainly detected in the packing bed of the hybrid system. Meanwhile, some facultative heterotrophic and dissimilated nitrate-reduction (DNRA) genera also co-existed. The profiling of key functional genes further proved concurrent occurrence of methanogenesis, aerobic methane oxidation and denitrification. Furthermore, possible microbial mechanism on AMODM process was elucidated from the prospective of targeted species interaction within the reactor. This research provides a robust and environment-friendly alternative process treating nitrate-containing organic wastewater towards efficient nitrogen removal, low resource consumption, bioenergy recovery and greenhouse gas reduction.

Combined genome-centric metagenomics and stable isotope probing unveils the microbial pathways of aerobic methane oxidation coupled to denitrification process under hypoxic conditions

Obligate aerobic methanotrophs have been proven to oxidize methane and participate in denitrification under hypoxic conditions. However, this phenomenon and its metabolic mechanism have not been investigated in detail in aerobic methane oxidation coupled to denitrification (AME-D) process. In this study, a type of hypoxic AME-D consortium was enriched and operated for a long time in a CH4-cycling bioreactor with strict anaerobic control and the nitrite removal rate reached approximately 50 mg N/L/d. Metagenomics combined with DNA stable-isotope probing demonstrated that the genus Methylomonas, which constitutes type I aerobic methanotrophs, was the dominant member and contributed to methane oxidation and partial denitrification. Metagenomic binning recovered a near-complete (98%) draft genome affiliated with the family Methylococcaceae containing essential genes that encode nitrite reductase (nirK), nitric oxide reductase (norBC) and hydroxylamine dehydrogenase (hao). Metabolic reconstruction of the selected Methylococcaceae genomes also revealed a potential link between methanotrophy and partial denitrification.

Large freshwater phages with the potential to augment aerobic methane oxidation

There is growing evidence that phages with unusually large genomes are common across various microbiomes, but little is known about their genetic inventories or potential ecosystem impacts. In the present study, we reconstructed large phage genomes from freshwater lakes known to contain bacteria that oxidize methane. Of manually curated genomes, 22 (18 are complete), ranging from 159 kilobase (kb) to 527 kb in length, were found to encode the pmoC gene, an enzymatically critical subunit of the particulate methane monooxygenase, the predominant methane oxidation catalyst in nature. The phage-associated PmoC sequences show high similarity to (>90%), and affiliate phylogenetically with, those of coexisting bacterial methanotrophs, including members of Methyloparacoccus, Methylocystis and Methylobacter spp. In addition, pmoC-phage abundance patterns correlate with those of the coexisting bacterial methanotrophs, supporting host–phage relationships. Future work is needed to determine whether phage-associated PmoC has similar functions to additional copies of PmoC encoded in bacterial genomes, thus contributing to growth on methane. Transcriptomics data from Lake Rotsee (Switzerland) showed that some phage-associated pmoC genes were highly expressed in situ and, of interest, that the most rapidly growing methanotroph was infected by three pmoC-phages. Thus, augmentation of bacterial methane oxidation by pmoC-phages during infection could modulate the efflux of this potent greenhouse gas into the environment.

Microbial utilization of rare earth elements at cold seeps related to aerobic methane oxidation

A major breakthrough in the field of rare earth element (REE) geochemistry has been the recent discovery of their utility to microbial life, as essential metalloenzymes catalyzing the oxidation of methanol to formaldehyde. Lanthanide-dependent bacteria are thought to be ubiquitous in marine and terrestrial environments, but direct field evidence of preferential microbial utilization of REE in natural systems is still lacking. In this study, we report on the REE and trace element composition of the tube of a siboglinid worm collected at a methane seep in the Gulf of Guinea; a tube-dwelling annelid that thrives in deep-sea chemosynthetic ecosystems. High-resolution trace element profiles along the chitin tube indicate marked enrichments of lanthanum (La) and cerium (Ce) in its oxic part, resulting in REE distribution patterns that depart significantly from the ambient seawater signature. Combined with various geochemical and microbiological evidence, this observation provides direct support for an active consumption of light-REE at cold seeps, associated with the aerobic microbial oxidation of methane. To further evaluate this hypothesis, we also re-examine the available set of REE data for modern seep carbonates worldwide. While most carbonate concretions at cold seeps generally display REE distribution patterns very similar to those for reduced pore waters in marine sediments, we find that seafloor carbonate pavements composed of aragonite commonly exhibit pronounced light-REE enrichments, as inferred from high shale-normalized La/Gd ratio (>~0.8), interpreted here as possibly reflecting the signature of lanthanide-dependent methanotrophic activity. This finding opens new perspectives for revisiting REE systematics in ancient seep carbonates and other microbialites throughout the Earth's history. In particular, the geochemical imprint of aerobic methane oxidation could be possibly traced using REE in Archaean stromatolites and other archives of Precambrian seawater chemistry, potentially providing new insights into the oxygenation of early Earth's oceans and associated microbiogeochemical processes.

The indispensable role of assimilation in methane driven nitrate removal

Methane is a greenhouse gas that can be released from sludge anaerobic fermentation in wastewater treatment plants. Methane is also an alternative additional carbon source for deep nitrate removal of secondary effluent. A sequencing experiment was conducted to study the efficacy of nitrate removal with methane as the sole carbon source. The maximum nitrate removal rate was 17.2 mg-N·L−1·d−1. Nitrate removal was confirmed to arise via two pathways: aerobic methane oxidation coupled to denitrification (AME-D) contributed to 55% of the nitrate removal with the rest stemming from assimilation by methanotrophs. Additional study revealed that nitrate assimilated by methanotrophs was used for the synthesis of proteins, resulting in a protein content of 52.2% dry weight. Metagenomic sequencing revealed a high abundance of nitrate assimilation and glutamine synthetase genes, which were primarily provided by methanotrophs (mainly Methylomonas). Assimilatory nitrate removal by methanotrophs has a high potential for advanced nitrogen removal and for alleviating methane emissions. The nitrogen-rich biomass produced by nitrate absorption could also be used as a biofertilizer for nitrogen recycling.

Surface Methane Concentrations Along the Mid‐Atlantic Bight Driven by Aerobic Subsurface Production Rather Than Seafloor Gas Seeps

AbstractRelatively minor amounts of methane, a potent greenhouse gas, are currently emitted from the oceans to the atmosphere, but such methane emissions have been hypothesized to increase as oceans warm. Here, we investigate the source, distribution, and fate of methane released from the upper continental slope of the U.S. Mid‐Atlantic Bight, where hundreds of gas seeps have been discovered between the shelf break and ~1,600 m water depth. Using physical, chemical, and isotopic analyses, we identify two main sources of methane in the water column: seafloor gas seeps and in situ aerobic methanogenesis which primarily occurs at 100–200 m depth in the water column. Stable isotopic analyses reveal that water samples collected at all depths were significantly impacted by aerobic methane oxidation, the dominant methane sink in this region, with the average fraction of methane oxidized being 50%. Due to methane oxidation in the deeper water column, below 200 m depth, surface concentrations of methane are influenced more by methane sources found near the surface (0–10 m depth) and in the subsurface (10–200 m depth), rather than seafloor emissions at greater depths.

Metagenome metabolic analysis revealing the mechanism of simultaneous methanogenesis, aerobic methane oxidation and denitrification (SMAMOD) in a microaerobic up‐flow sludge bed biofilm reactor

AbstractBACKGROUNDImproved denitrification with methane as alternative electron donor is regarded as a promising low‐cost approach for organic nitrate‐containing wastewater treatment. However, in situ capture of methane released from a methanogenic system for enhancing nitrogen removal seems to be scarcely reported. The applications of simultaneous denitrification, methanogenesis, and aerobic methane oxidation coupled to denitrification provide the possibility for solving the challenging issue.RESULTSIn this study, simultaneous methanogenesis, aerobic methane oxidation, and denitrification (SMAMOD) was firstly established in a microaerobic up‐flow sludge bed biofilm reactor (MUSBBR) treating high‐strength synthetic wastewater with 2000 mg L−1 Chemical oxygen demand (COD) and 400 mg L−1 nitrate. The operation demonstrated 98.8% COD, 98.8% NO3−‐N, and 93.5% TN removals were stably obtained. Microbial community found the coexistence and cooperation of Methanothrix as methanogens, Methylogaea as aerobic methanotrophs, as well as facultative denitrification genera in SMAMOD system. Gene profiling demonstrated key functional genes involved in aerobic and anaerobic fermentation, methanogenesis and aerobic methane oxidation, as well as nitrate reduction induced by nitrate reductase systems. In organic oxygen‐limited biotransformation, glucose was degraded mainly through aerobic respiration, anaerobic fermentation into methane by decarboxylation of acetic acid, and further methane aerobic oxidation pathways, while acetate‐ and methanol‐denitrification and dissimilatory nitrate reduction to ammonium pathways participated in the nitrogen bioconversion. Further, the metabolic mechanism of SMAMOD might be unraveled by spatial interactions of key metagenomes in core microbial consortiums in the MUSBBR reactor.CONCLUSIONThe study offers valuable metagenomic insights into SMAMOD as a potentially compact process for sustainable treatment of high‐strength organic nitrate‐containing wastewater. © 2020 Society of Chemical Industry

Ammonium Impacts Methane Oxidation and Methanotrophic Community in Freshwater Sediment.

Lacustrine ecosystems are regarded as one of the important natural sources of greenhouse gas methane. Aerobic methane oxidation, carried out by methane-oxidizing bacteria, is a key process regulating methane emission. And ammonium is believed to greatly influence aerobic methane oxidation activity. To date, disagreement exists in the threshold of ammonium effect. Moreover, knowledge about how aerobic methanotrophic community composition and functional gene transcription respond to ammonium is still lacking. In the present study, microcosms with freshwater lake sediment were constructed to explore the effect of ammonium level on aerobic methanotrophs. Methane oxidation potential, and the density, diversity and composition of pmoA gene and its transcripts were examined during 2-week incubation. A negative impact of ammonium on aerobic methane oxidation potential and a positive impact on pmoA gene density were observed only at a very high level of ammonium. However, pmoA gene transcription increased notably at all ammonium levels. The composition of functional pmoA gene and transcripts were also influenced by ammonium. But a great shift was only observed in pmoA transcripts at the highest ammonium level.

Simultaneous nitrogen and dissolved methane removal from an upflow anaerobic sludge blanket reactor effluent using an integrated fixed-film activated sludge system

One of the main drawbacks of upflow anaerobic sludge blanket (UASB) reactors that treat low-strength sewage at room temperature is related to the low quality of their effluents in terms of dissolved methane, organic matter, and nitrogen content. The present study aims to evaluate the feasibility of using an integrated fixed-film activated sludge (IFAS) system as an alternative post-treatment technology to mitigate the environmental impact of such effluents. For this purpose, a pilot plant composed of a UASB (120 L) followed by an IFAS (66 L) system was operated for 407 days. Special attention was paid to the suspended biomass retention capacity and the dissolved methane and nitrogen removal potential of the IFAS post-treatment system. Furthermore, the role of carriers on denitrification and nitrification processes and the microbial communities present in the biofilm were also analyzed. Average total chemical oxygen demand (CODT) and ammonium removal efficiencies of 92 ± 3% and around 57 ± 16% were attained throughout the entire operation, respectively. During a first period in which biomass was maintained in both biofilms and suspension, and nitrite was the main electron acceptor, maximum nitrogen removal and methane removal efficiencies of 32.5 mg TN L-1 and 93% were observed in the IFAS system, respectively. However, throughout the second period, in which suspended biomass was completely washed out from the IFAS system, and nitrate became the main electron acceptor, these values decreased to 18 ± 4 mg TN Lfeed-1 and 77 ± 12%, respectively. Surprisingly, throughout the entire operation, it was observed that around 50 and 41% of the total nitrogen and methane removals observed in the IFAS system, respectively, were carried out in the aerobic compartment. Aerobic methane oxidizers and anammox were detected with significant relative abundances in the biofilm carriers used in the anoxic and aerobic compartments using 16S rRNA gene amplicon sequencing analysis. Therefore, the use of an IFAS system could be suited to diminish greenhouse gas emissions and nutrients concentration for those sewage treatment plants that used UASB systems, especially in countries with temperate and warm climates.

Methane oxidation dynamics in a karst subterranean estuary

Chemical gradients between fresh, brackish and saline waters shape biogeochemical reactions and organic matter transformation within subterranean estuaries. In the Yucatán Peninsula’s karst subterranean estuary (KSE), methane and dissolved organic matter generated during the anaerobic decomposition of tropical forest vegetation are transported into flooded cave networks where microbial consumption greatly reduces their concentrations in the groundwater. To test the hypothesis that chemoclines associated with salinity gradients of the KSE are sites of methane oxidation, we obtained methane concentration and δ13C profiles of unprecedented vertical resolution from within a fully-submerged cave system located 6.6 km inland from the coastline using the ‘OctoPiPi’ (OPP) water sampler. Along a 12–24 cm thick low-salinity-halocline at ∼4.5 m water depth, salinity increased from fresh to brackish (0.2–1.8 psu), methane concentrations decreased, and δ13C values increased, as expected for microbial methane oxidation. The underlying brackish water had elevated oxygen concentrations compared to the always anoxic freshwater, suggesting that aerobic methane oxidation is the dominant process facilitating methane consumption. By contrast, as salinity increased from 1.8 to 36 psu through a 24–36 cm thick high-salinity-halocline between the meteoric lens and the saline groundwater at ∼20 m water depth, methane concentrations and δ13C values were constant. Conservative mixing and kinetic isotope models incorporating the methane data confirm a hotspot for microbial methane oxidation at the low-salinity-halocline. At least 98% of methane originating in the anoxic freshwaters was removed before its transport via channelized flow towards the coastline. These findings provide novel insight into the spatial constraints of methane dynamics within a karst subterranean estuary.

Diversity of active root-associated methanotrophs of\xa0three\xa0emergent\xa0plants in\xa0a eutrophic wetland in northern China

Root-associated aerobic methanotrophs play an important role in regulating methane emissions from the wetlands. However, the influences of the plant genotype on root-associated methanotrophic structures, especially on active flora, remain poorly understood. Transcription of the pmoA gene, encoding particulate methane monooxygenase in methanotrophs, was analyzed by reverse transcription PCR (RT-PCR) of mRNA isolated from root samples of three emergent macrophytes, including Phragmites australis, Typha angustifolia, and Schoenoplectus triqueter (syn. Scirpus triqueter L.) from a eutrophic wetland. High-throughput sequencing of pmoA based on DNA and cDNA was used to analyze the methanotrophic community. Sequencing of cDNA pmoA amplicons confirmed that the structure of active methanotrophic was not always consistent with DNA. A type I methanotroph, Methylomonas, was the most active group in P. australis, whereas Methylocystis, a type II methanotroph, was the dominant group in S. triqueter. In T. angustifolia, these two types of methanotroph existed in similar proportions. However, at the DNA level, Methylomonas was predominant in the roots of all three plants. In addition, vegetation type could have a profound impact on root-associated methanotrophic community at both DNA and cDNA levels. These results indicate that members of the genera Methylomonas (type I) and Methylocystis (type II) can significantly contribute to aerobic methane oxidation in a eutrophic wetland.

Methanol-linked synergy between aerobic methanotrophs and denitrifiers enhanced nitrate removal efficiency in a membrane biofilm reactor under a low O2:CH4 ratio

Nitrate removal efficiency of aerobic methane oxidation coupled with denitrification (AME-D) process was elevated by enhancing the methanol-linked synergy in a membrane biofilm reactor (MBfR) under a low O2:CH4 ratio. After 140 days’ enrichment, the nitrate removal rate increased significantly from 3 to 4 mg-N L−1 d−1 to 22.09 ± 1.21 mg-N L−1 d−1 and the indicator, mol CH4 consumed/mol reduced NO3−-N (C/N ratio), decreased to 1.79 which was very close to the theoretical minimum value (1.27–1.39). The increased nitrate removal efficiency was largely related to the enhanced relationship between aerobic methanotrophs and methanol-utilizing denitrifiers. Type I methanotrophs and some denitrifiers, especially those potential methanol-utilizing denitrifiers from Methylobacillus, Methylotenera, Methylophilus and Methyloversatilis, were abundant in the MBfR sludge. Aerobic methanotrophs and potential methanol-utilizing denitrifiers were closely associated in many globular aggregates (5–10 μm diameter) in the MBfR sludge, which may have promoted the denitrifiers to capture methanol released by methanotrophs efficiently. If we assume methanol is the only cross-feeding intermediate in the MBfR, about 38–60% of the CH4 supplied would be converted to methanol and secreted rather than continuing to be oxidized. At least 63% of this secreted methanol should be utilized for denitrification instead of being oxidized by oxygen in the MBfR. These findings suggest that the nitrate removal efficiency of the AME-D process could be significantly improved.

Effects of oxygen tension on the microbial community and functional gene expression of aerobic methane oxidation coupled to denitrification systems.

Aerobic CH4 oxidation coupled to denitrification (AME-D) can not only mitigate the emission of greenhouse gas (e.g., CH4) to the atmosphere, but also reduce NO3- and/or NO2- and alleviate nitrogen pollution. The effects of O2 tension on the community and functional gene expression of methanotrophs and denitrifiers were investigated in this study. Although higher CH4 oxidation occurred in the AME-D system with an initial O2 concentration of 21% (i.e., the O2-sufficient condition), more NO3--N was removed at the initial O2 concentration of 10% (i.e., the O2-limited environment). Type I methanotrophs, including Methylocaldum, Methylobacter, Methylococcus, Methylomonas, and Methylomicrobium, and type II methanotrophs, including Methylocystis and Methylosinus, dominated in the AME-D systems. Compared with type II methanotrophs, type I methanotrophs were more abundant in the AME-D systems. Proteobacteria and Actinobacteria were the main denitrifiers in the AME-D systems, and their compositions varied with the O2 tension. Quantitative PCR of the pmoA, nirS, and 16S rRNA genes showed that methanotrophs and denitrifiers were the main microorganisms in the AME-D systems, accounting for 46.4% and 24.1% in the O2-limited environment, respectively. However, the relative transcripts of the functional genes including pmoA, mmoX, nirK, nirS, and norZ were all less than 1%, especially the functional genes involved in denitrification under the O2-sufficient condition, likely due to the majority of the denitrifiers being dormant or even nonviable. These findings indicated that an optimal O2 concentration should be used to optimize the activity and functional gene expression of aerobic methanotrophs and denitrifiers in AME-D systems.

Disentangling abiotic and biotic controls of aerobic methane oxidation during re-colonization

Aerobic methane oxidation is driven by both abiotic and biotic factors, which are often confounded in the soil environment. Using a laboratory-scale reciprocal inoculation experiment with two native soils (paddy and upland agricultural soils) and the gamma-irradiated fraction of these soils, we aim to disentangle and determine the relative contribution of abiotic (i.e., soil edaphic properties) and biotic (i.e., initial methanotrophic community composition) controls of methane oxidation during re-colonization. Methane uptake was appreciably higher in incubations containing gamma-irradiated paddy than upland soil despite the initial difference in the methanotrophic community composition. This suggested an overriding effect of the soil edaphic properties, which positively regulated methane oxidation. Community composition was similar in incubations with the same starting inoculum, based on quantitative and qualitative pmoA gene analyses. Thus, results suggested that the initial community composition affects the trajectory of community succession to an extent, but not at the expense of the methanotrophic activity under high methane availability. Still, methane oxidation was affected more by soil edaphic properties than by the initial composition of the methanotrophic community.

Highly selective aerobic oxidation of methane to methanol over gold decorated zinc oxide via photocatalysis

Au/ZnO is employed as a photocatalyst for methane oxidation to methanol with 100% selectivity in an oxygen atmosphere at atmospheric temperature.

Investigations of Aerobic Methane Oxidation in Two Marine Seep Environments: Part 1—Chemical Kinetics

AbstractMicrobial aerobic oxidation is known to be a significant sink of marine methane (CH4), contributing to the relatively minor atmospheric release of this greenhouse gas over vast stretches of the ocean. However, the chemical kinetics of aerobic CH4 oxidation are not well established, making it difficult to predict and assess the extent that CH4 is oxidized in seawater following seafloor release. Here we investigate the kinetics of aerobic CH4 oxidation using mesocosm incubations of fresh seawater samples collected from seep fields in Hudson Canyon, U.S. Atlantic Margin and MC118, Gulf of Mexico to gain a fundamental chemical understanding of this CH4 sink. The goals of this investigation were to determine the response or lag time following CH4 release until more rapid oxidation begins, the reaction order, and the stoichiometry of reactants utilized (i.e., CH4, oxygen, nitrate, phosphate, trace metals) during CH4 oxidation. The results for both Hudson Canyon and MC118 environments show that CH4 oxidation rates sharply increased within less than one month following the CH4 inoculation of seawater. However, the exact temporal characteristics of this more rapid CH4 oxidation varied based on location, possibly dependent on the local circulation and biogeochemical conditions at the point of seawater collection. The data further suggest that methane oxidation behaves as a first‐order kinetic process and that the reaction rate constant remains constant once rapid CH4 oxidation begins.