Advanced Research Research Articles

Peritoneal Carcinomatosis and Situs Inversus Totalis: A Rare Case Report

Gastric cancer remains one of the foremost important cancers around the world because it positions as the fifth most common cancer and the fourth driving cause of cancer passing all-inclusive. Situs inversus totalis (SIT) alludes to a turned-around area of the stomach and thoracic organs. The heart is found on the correct side of the chest (dextrocardia) with a total inversion of the heart chambers. The ordinary pulmonary anatomy is additionally turned around, so the cleared-out lung has three flaps and the proper lung has two flaps. In addition, the liver and gallbladder are found on the cleared-out side of the guts, though the spleen and stomach are found on the proper side. The remaining internal structures are moreover a reflected picture of the typical. This natural irregularity is outstandingly remarkable with a pace of one case in 10,000 births. SIT might be a phenomenal intrinsic irregularity that stances unique difficulties amid careful organization. Preoperative organizing, counting imaging and the mindful idea of careful techniques, are fundamental for powerful outcomes in SIT patients with gastric disease. The use of laparoscopic and automated procedures has seemed, by all accounts, to be secure and practical in this calm people. Advanced research is expected to push ahead the comprehension of the aetiology and optimal organization of SIT patients with gastric disease. SIT could be a remarkable innate eccentricity that stances unique difficulties during careful organization. Preoperative orchestrating, counting imaging and the wary idea of surgeries, are crucial for productive outcomes in SIT patients with gastric disease. Advanced careful fitness is expected to play out a definite lymphadenectomy in calm with SIT by envisioning the exact reflected image of the existing frameworks during the activity. The diligent had an ordinary intra-and postoperative course and was taken after up at the short-term division with no demonstration of rehash. All in all, medical procedures in comprehension with peritoneal disease and SIT can be safely performed by paying thought to the improved anatomic designs amid the activity.

Innovative insight: ChatGPT's contribution to advanced health research articles

The current technological advancement follows a pattern of both disruptive and gradual changes. The field of communication is currently the most extensively utilized technology. The rapid development of AI has enabled those without a background in computer coding or specialized health knowledge to utilize this technology, which gives rise to numerous issues. This phenomenon elicits curiosity and engenders debate among the general public, scholars, healthcare providers, and researchers in the health industry. Utilizing AI in the composition of scientific publications necessitates explicit norms and regulations, a robust validation procedure, and effective collaboration between AI systems and human expertise. Ensuring transparency and acknowledgment of AI usage is crucial to maintaining human researchers' accountability for the ultimate outcomes of their research.

The Study of a Kazakh document in Chinese archives related to Khankhoja sultan’s cattle trade and its historical significance

In the following article, Advanced research methods of history and historical philology are used, along with a letter written by the Kazakh’s sultan Khankhoja, who owned the East Kazakh steppe since the end of the 18thcentury and had close relations with the neighboring Qing dynasty, to the local taiji who was in charge of af-fairs in Tarbagatai is studied. The letter is written in the old Kazakh written language, the language is simple, understandable, the content is deep, it is an original document written by the Kazakh, which is very valuable for the comprehensive study of the trade business of the Kazakh steppe. In the previous studies, it was often said that “silk was traded for horses”, but as we can see from the content of the analyzed letter, not only hors-es were exchanged for silk, sometimes other livestock were also traded. The letter is now stored in the First Historical Archives of China in Beijing. From the contents of the letter, the long history of Kazakh- Kyrgyz relations can be indirectly understood.

A novel attention model across heterogeneous features for stuttering event detection

Stuttering is a prevalent speech disorder affecting millions worldwide. To provide an automatic and objective stuttering assessment tool, Stuttering Event Detection (SED) is under extensive investigation for advanced speech research and applications. Despite significant progress achieved by various machine learning and deep learning models, SED directly from speech signal is still challenging due to stuttering speech’s heterogeneous and overlapped nature. This paper presents a novel SED approach using multi-feature fusion and attention mechanisms. The model utilises multiple acoustic features extracted based on different pitch, time-domain, frequency domain, and automatic speech recognition feature to detect stuttering core behaviours more accurately and reliably. In addition, we exploit both spatial and temporal attention mechanisms as well as Bidirectional Long Short-Term Memory (BI-LSTM) modules to learn better representations to improve the SED performance. The experimental evaluation and analysis convincingly demonstrate that our proposed model surpasses the state-of-the-art models on two popular stuttering datasets, with 4% and 3% overall F1 scores, respectively. The superior results indicate the consistency of our proposed method, supported by both multi-feature and attention mechanisms in different stuttering events datasets.

Assessment of conservation status of riparian vascular plant species in a dry season exposed flood plain area of the Incalaue river catchment, Niassa Special Reserve, Northern Mozambique

Riparian vegetation usually gets less focus in biodiversity assessments and yet species diversity is important knowledge when applying patch specific conservation value in the Niassa Special Reserve (NSR). This study assessed the composition and conservation status of riparian species in an exposed river basin downstream location. Purposive sampling was used in the selection of sites and respondents to maximize data collection. The study found 19 species belonging to 15 families with 52.63% of them having a frequency of ⩾50% in sampling plots. There were 10 species that are endemic to the sub-Sharan Africa Region. Fabaceae was the dominant family with 5 species. The species with the highest population was Flacourtia indica (Burm. f.) Merr. Species richness ranged from 0.35 to 0.98 with a mean of 0.66 ± 0.22. The IVI ranged from 34.70 (F. indica (Burm. f.) Merr) to 4.43 (Tribulus cistoides L.) with a mean of 15.79 ± 7.79. Threats of species loss and ecosystem disturbance were agriculture, infrastructure development and plant harvests. There was a reported decline in species availability over the previous 10 years by 18.7% of the respondents. The results added to existing studies and records of vegetation species of conservation value in areas exposed to loss in the NSR. This study advances research on vegetation range dynamics in the NSR and presents a need to mitigate human land use impacts on riparian vegetation species composition.

Accelerating Electrocatalyst Innovation: High-Throughput Automated Microkinetic Modeling

To reduce energy-related emissions, we must create chemicals from carbon dioxide with renewable energy, instead of from petroleum. Designing such a process needs computer models that can describe all the chemical reactions that are happening, including those driven by electrochemistry. There may be thousands of such reactions, so we must build a tool to find them automatically. Our reaction mechanism generator for electrocatalysis will create detailed kinetic models for many electrochemical processes, but for this initial project we are targeting the reduction of carbon dioxide to produce propanol, a useful hydrocarbon product. The kinetic models will be used to simulate an electrochemical reactor, then analyzed both technically and economically to determine which catalyst materials and reactor designs are best. This will allow new catalyst materials to be discovered using powerful computers, instead of using very time-consuming and expensive experiments.Building on the state-of-the-art open-source Reaction Mechanism Generator (RMG) software [1,2], we are creating the first automated mechanism generator for electrochemical reactions on a catalyst. Supplied with a feedstock, catalyst, and conditions, it will propose a detailed kinetic model comprising hundreds of intermediate species and reactions. Reactor simulations will predict product yields as a function of feed, concentrations, and potential, allowing conditions to be optimized and the trade-off between conversion and selectivity to be investigated. In turn this could inform a detailed technoeconomic analysis, enabling catalyst materials to be screened in silico on an economic basis. Meanwhile sensitivity analysis of the simulations will select estimated parameters for refinement with DFT calculations.We must first extend RMG to allow charged species, and implement proton coupled electron transfer (PCET) reactions. We will at first use the computational hydrogen electrode model to determine the Gibbs energy of a species (and hence reaction) as a function of chemical potential. Potential-dependent kinetics will be estimated using a charge transfer coefficient model. In preliminary work with a framework like this we have constructed a reaction mechanism for carbon dioxide reduction on Cu(111) with 37 species and 292 reactions (23 of them electrochemical) [3]. The model discovered many key electrochemical pathways to reduce CO2 to experimentally observed products such as methane, methanol, formic acid, ethylene, and ethanol. Acknowledgements The information, data, or work presented herein was funded in part by the Advanced Research Projects Agency-Energy (ARPA-E), U.S. Department of Energy, under Award Number DE-AR0001786. The authors thank Dr. David Farina Jr. for his significant contributions to the proof-of-concept.

(Invited) Electrochemistry in a Lab of the Future

Advanced research laboratories are producing ever more data and introducing more advanced methods of data analysis and feedback to control these laboratories. These "labs of the future" are intended to revolutionize the way that research is done, enabling in some cases the exploration of higher dimensional problems and better research outcomes. At ORNL, the Autonomous Chemistry Laboratory (ACL) is being developed as a self-driving laboratory to accelerate materials discovery and innovation. A combination of robotic tools are being integrated with computational tools to analyze reaction results and predict optimal directions for future investigation. We have developed an electrochemical workstation integrated with materials synthesis and discovery. The capabilities and concept for the ACL will be presented.

(Invited) In-Situ MOCVD Etching of GaN Using XeF2 for Selective-Area-Epitaxial-Regrowth of p-Type GaN for High Voltage PN Diodes

PN diodes with multi-kilovolt breakdown voltages have been demonstrated to great than 6 kV in GaN, validating the device potential predicted by the intrinsic material properties of III-nitride semiconductors. However, power control circuits also require switching transistors such as junction field effect transistors (JFETs) and practical diodes such as merged PIN Schottky (MPS) diodes. Such devices usually employ selective areas of p-type semiconductor surrounded by n-type material and require the formation of PN junctions with low reverse leakage current. Selective-area p-type doping in Si and SiC based power devices is typically achieved using implantation and thermal annealing to form the p-type areas. Ion implantation of Mg in GaN has demonstrated p-type conductivity, but this process requires specialized equipment for the high-pressure and high-temperature activation of Mg dopants. We have investigated selective-area-regrowth (SArG) to epitaxially grow p-type GaN as an alternative to dopant implantation. Successful PN diode formation by SArG requires a process to remove residual crystalline damage resulting from the ex-situ inductively coupled plasma (ICP) etch typically used to form the p-well, as well as a method to remove the elevated level of Si found at the regrowth interface of a surface that had been exposed to air. For the case of SArG of p-GaN on air-exposed, blanket ICP etched n-type GaN, we present the novel use of a fluorine-based precursor (XeF2) for in-situ etching of GaN in the MOCVD chamber. Unlike chlorine (TBCl and CCl4) and bromine (CBr4) -based precursors we have studied; we obtained a smooth surface following in-situ fluorine etching of air-exposed GaN. Schottky barrier diodes (SBDs) formed by shadow mask evaporation on in-situ fluorine etched n-GaN that had been previously ICP etched showed reverse leakage currents to -40 V that are equal to those of SBDs formed on as-grown n-GaN layers. The in-situ fluorine/ICP etched Schottky diodes had reverse leakage currents more than 3 orders of magnitude lower than those formed on n-GaN layers that had only experienced ICP etching. This suggests that the in-situ fluorine etch was effective at removing the residual damage from the ICP etch process. Furthermore, we discuss the use of in-situ fluorine etching to reduce the concentration of Si at the regrowth interface required to form PN junctions with low reverse leakage current. This work was supported in part by the Advanced Research Projects Agency – Energy (ARPA-E), U.S. Department of Energy under the PNDIODES program directed by Dr. Isik Kizilyalli, and in part by the US Department of Energy (DOE) Vehicle Technologies Office (VTO) under the Electric Drive Train Consortium. Sandia National Laboratories is a multimission laboratory managed and operated by National Technology and Engineering Solutions of Sandia, LLC., a wholly owned subsidiary of Honeywell International, Inc., for the U.S. Department of Energy’s National Nuclear Security Administration under contract DE-NA-0003525. The views expressed in the presentation do not necessarily represent the views of the U.S. Department of Energy or the United States Government.

(Invited) Selective Area Growth, Etching, and Doping of GaN By MOCVD for Power Electronics

Gallium Nitride (GaN) possesses a wide band gap and excellent transport properties, making it a highly promising material for high-power and high-frequency applications. Microwave power amplification for commercial and military purposes has already benefited from GaN-based high electron mobility transistors (HEMTs). However, currently available GaN optoelectronic and electronic devices use planar junctions and heterostructures prepared by epitaxy, typically via metalorganic chemical vapor deposition (MOCVD). To enable more sophisticated device configurations, in-plane lateral junctions are necessary. The most effective method for achieving this is through selective area etching (SAE) followed by selective area growth (SAG). In this talk, we will discuss the progress made at Yale in the selective-area etching, growth, and doping of GaN.Selective-area etching of GaN is the most challenging and critical step in this process. Instead of using conventional ICP etching, we have employed tertiarybutylchloride (TBCl) as an in-situ chemical etchant of GaN in MOCVD to create smooth trenches and remove plasma etching-generated damages on the surface. With electrical and material characterizations, we have confirmed that TBCl etching is a low-damage and clean etching process. We will also describe our recent efforts to bypass the constraint of dielectric masking during in-situ etching and perform maskless selective area etching (SAE) to create low-defect lateral p-n junctions for GaN power electronics. Additionally, we will discuss non-planar selective-area growth and doping, including the principles of growth evolution, non-uniform Mg distribution, and parasitic impurities.This work is supported by the Advanced Research Projects Agency-Energy (ARPA-E), U.S. DOE, under DE-AR0000871 as part of the PNDIODES program managed by Dr. Isik Kizilyalli.

AT THE NEXUS OF PUBLIC POLICY AND AGING: DOCTORAL STUDIES IN GERONTOLOGY AT THE UNIVERSITY OF MASSACHUSETTS BOSTON

Abstract One in five PhDs in Gerontology are graduates of the Gerontology doctoral program at the University of Massachusetts (UMass) Boston. The mission of UMass Boston’s program is to provide advanced training and research in gerontology within an interdisciplinary framework drawn primarily, but not exclusively, from the social and behavioral sciences. The program is designed to prepare students for leadership roles as educators, researchers, planners, and policy makers. The program has a deep emphasis on policy development, analysis, and impact, an attribute that goes back to the roots of the Gerontology enterprise at UMass Boston in the late-1970s. This presentation provides an overview of the program’s history and curriculum, along with coursework, research and service-learning experiences, and career paths in policy research and implementation. The program also is committed to increasing diversity in the field of gerontology, with the aim of strengthening policy impact and reducing disparities in the aging experience.

История Сибирского государственного университета геосистем и технологий через призму геодезической фалеристики

The author presents historical facts from the period of formation and development of the Siberian State University of Geosystems and Technologies (from 1939 to 1994 – Novosibirsk Institute of Geodesy, Aerial Photography and Cartography Engineers), the only geodetic university beyond the Urals. Biographies of some professors and rectors, distinguished at development of the University, who carried out advanced scientific research or improved the pedagogical process and geodetic industry, are presented. The role of rector K. L. Provorov in enriching the material base of the institute and his contribution to geodetic science are reflected. He was one of the first in the country to have received the title Honored Figure of Geodesy and Cartography. There is some information on industry awards and honorary titles assigned to this educational institution’s employees and graduates. The most outstanding graduates submitted to State award, as well as those who became laureates of that named after F.N. Krasovsky for the best scientific and technical developments in geodesy and cartography are listed

Design for circularity in manufacturing industries–operationalisation and decision support

Achieving a more circular economy can be an important contributor to sustainable development. The successful implementation at the industrial level requires the development of circular products, services and business models. This paper addresses the compelling need for industrial product development to consider the entire life cycle of products from raw material extraction to the end of life to initiate and evaluate circular economy improvements such as lifetime extension or high-quality recycling. The actual implementation of circular economy in industrial product design processes hasn't yet been extensively researched. This paper operationalises a comprehensive design for circularity by conceptualising a decision support method for the industrial product development process based on literature analysis, decision matrix methodology, and expert evaluation. Results highlight the interplay of circular business models, ecodesign approaches, and product life cycle intensities and shape the basis for technical circular design principles and metrics as well as advanced implementation-oriented research in the field.

Parents’ hope in perinatal and neonatal palliative care: a scoping review

BackgroundThe diagnosis of a life-limiting condition of a child in the perinatal or neonatal period is a threat to parental hopes. Hope is an interactional and multidimensional construct, and in palliative care, it is a determinant of quality of life, survival, acceptance and peaceful death.ObjectiveTo map scientific evidence on parents’ hope in perinatal and neonatal palliative care contexts.Methoda scoping review theoretically grounded on Dufault and Martocchio’s Framework, following the Joanna Briggs Institute methodological recommendations. Searches were performed until May 2023 in the MEDLINE, CINAHL and PsycINFO databases. The searches returned 1341 studies.ResultsEligible papers included 27 studies, most of which were carried out in the United States under a phenomenological or literature review approach. The centrality of women’s perspectives in the context of pregnancy and perinatal palliative care was identified. The parental hope experience is articulated in dealing with the uncertainty of information and diagnosis, an approach to which interaction with health professionals is a determinant and potentially distressful element. Hope was identified as one of the determinants of coping and, consequently, linked to autonomy and parenthood. Cognitive and affiliative dimensions were the hope dimensions that predominated in the results, which corresponded to the parents’ ability to formulate realistic goals and meaningful interpersonal relationships, respectively.ConclusionHope is a force capable of guiding parents along the path of uncertainties experienced through the diagnosis of a condition that compromises their child’s life. Health professionals can manage the family’s hope by establishing sensitive therapeutic relationships that focus on the dimension of hope. The need for advanced research and intervention in parental and family hope are some of the points made in this study.Protocol registrationhttps://osf.io/u9xr5/.

Comparative insights of soil properties of termite hill in relation to the microbial community using culture-independent approach

The unique variety of soil enzymes found in termite mound soil results in different physico-chemical properties than the surrounding field soil. Metagenomic research of termite mound soil samples has paved a critical path in our comprehension of the ecosystem's diverse bacterial species. Despite advanced soil research, basic analytical tests on termite mound soil have not been considered. In the present study, microbial diversity of the two-termite mound soil was studied using Illumina Miseq sequencing by targeting V3-V4 regions in 16S rRNA. In termite mound soil (TMS) and adjacent field soil (AFS), the maximum bulk density, particle density, and porosity were 1.82 mg m−3, 3.33 mg m−3, 65 % and 1.25 mg m−3, 2.5 mg m−3, 50 %, respectively. The available NPK status in termite mound soil was higher than in surrounding field soil. The most abundant phylum in both samples was Proteobacteria, with abundances of 22.83 % and 32.76 % for samples R1 and R2, respectively. A noteworthy finding is that a small portion of the relative abundance is classified in the Kingdom Archaea identified upto species level of taxonomic classification.

Research Advanced in Pedestrian Detection Based on Deep Learning

In the field of computer vision, pedestrian detection is a significant research area that is frequently applied in areas like intelligent driving and video surveillance. The reliability and accuracy of subsequent tasks like pedestrian tracking and behavior analysis are directly impacted by the accuracy and effectiveness of pedestrian detection. However, there are still a number of difficulties with pedestrian detection tasks because of the variety of factors, including the shape, scale, and posture of pedestrians, as well as background interference and changes in lighting. Based on research on pertinent domestic and international literature, this article examines the key techniques for identifying pedestrians. Introduce frequently used datasets for pedestrian detection first. The model can then be split into two categories based on its training process: one-stage detection algorithms and two-stage detection algorithms. For each stage type, this study offers a thorough introduction to representative algorithms. A summary of its current state of development and future prospects was then given.

Research Advanced in 2D Multi-Object Tracking based on Deep Learning

Multi-object tracking is one of the most important research orientation of computer vision, which has a broad applications in the fields of sports game data analysis, video monitoring, pedestrian behavior analysis, and automatic driving. With the rapid-developed convolutional neural network, deep learning-based multi-object tracking methods have made significant progress. In this research, the current multi-object tracking methods are classified, analyzed and summarized through comprehensive and thorough research. Firstly, the definition of multi-object tracking, research background and application advantages of deep learning are introduced. Next, 2D multi-object tracking and 3D multi-object tracking as well as their differences are briefly introduced. Then, different methods in 2D multi-object tracking are explored in detail. The commonly used evaluation metrics and datasets are also introduced, as well as the comparison of the experimental results of different methods on the datasets. Finally, the advantages and disadvantages of multi-object tracking methods are analyzed and the future development direction is prospected. The development of multi-object tracking is of great significance for enhancing social security as well as promoting technological progress.

Investigating the Culture Around Sexual Harassment in First-Generation Universities in Southwestern Nigeria: Protocol for a Mixed Methods Study.

The phenomenon of sexual harassment (SH) is a complex issue with multiple prongs that concerns all members of academia and raises serious challenges, particularly regarding prevention and response. SH in tertiary institutions remains a huge problem worldwide, leading to severe emotional, academic, and career difficulties, as well as undue suffering. Institutions have responded in various ways to alleviate the burden of SH with little success, especially in Nigeria. The prevalence is high but reportage is low because of the culture of silence around SH in most educational institutions. This study aims to identify factors associated with SH in tertiary institutions in Nigeria and explore factors surrounding reportage or nonreportage following the experience of SH, the institutional mechanisms to prevent and respond to SH, and the lived experience of survivors of SH. The objective of this study was to present a study protocol that is designed to identify factors associated with the experience of SH in tertiary institutions in Nigeria, the institutional mechanisms to prevent and respond to SH, and the lived experience of survivors of SH. A mixed (quantitative and qualitative) methods approach is used consisting of a policy review of existing antisexual harassment policies in the selected universities, a quantitative survey to determine the correlates of SH, focus group discussions to explore the perspectives of the university community concerning SH, in-depth interviews to explore the lived experiences of survivors of SH, and key informant interviews to understand the perspectives of people who provide interventions to survivors. This study was funded in July 2022 by the Consortium for Advanced Research Training in Africa, and data collection started in November 2022. The SH policies were comprehensive, with clear policy statements and definitions, and recognized a wide range of survivors and perpetrators. However, there was no clear mention of prevention and response to same-sex SH. Lived experiences showed negative psychological and social sequelae and little institutional support. This is the first study that has a component investigating same-sex SH in tertiary institutions in Nigeria. This is also one of the first studies to explore the lived experiences of survivors of SH in Nigerian universities. The findings from this study suggest that periodic evaluation of SH policy implementation will improve institutional support, thus creating safe spaces for survivors and will thereby encourage reportage and support; prevention and response strategies need to be more inclusive; and more interventions should focus on strengthening prosocial skills and healthy, equitable relationships. DERR1-10.2196/49126.

Effectiveness of Nurse’s Knowledge and Practices Program Toward Care of Patient Undergoing Cardiopulmonary Bypass

Abstract
 Objective : the aim of the study is to evaluate the effectiveness of nurse’s practices and knowledge program toward care of patient with cardiopulmonary bypass .Methodology: A pre-experimental design (one group pretest- posttest design) was used to carry out this study in three governmental hospitals in Baghdad ( Iraqi Centre for Heart Diseases , Ibn – Albitar Centre for Cardiac Surgery and Ibn – Alnafees Centre for Cardiothoracic and Vascular Surgery ) for the period from 15th of May 2022 into 20th of April 2023.The construction and development of the program and instruments had carry out by the researcher to measured the purposes of the study , twenty two nurses was purposively assigned for the study group . The study instrument consisted from three parts ,socio – demographic data that consist from ( 4 ) items , knowledge part that consist from ( 25 ) items , and observational checklist for practices part that consist from ( 26 ) items . Descriptive and inferential statistics was used to analyse the study data .Results : The results of the study presented that effect of nurses practices and knowledge program toward care of patient with cardiopulmonary bypass was positive with highly significant level between pretest and posttest for knowledge and practices. Conclusion :Nursing care for the patient with cardiopulmonary bypass have been improved related to the enhancement of nursing staff knowledge and practices toward cardiopulmonary bypass.Recommendations : The study recommended to apply this program as apart of continues education in hospital that perform cardiac surgery in Iraq , also encourage the ministry of health to provide educational programs that depending on standards guidelines and advanced research on cardiopulmonary bypass .
 
 
 
 
 
 

Cissus antractica-ZnO NPs Induce Apoptosis in A549 Cells through ROS-Generated p53/Bcl-2/Bax Signaling Pathways and Inhibition of Inflammatory Cytokines

Biogenic synthesis using medicinal plants has less harmful effects as compared to the chemical synthesis of nanoparticles. Here, for the first time, we successfully demonstrated the eco-friendly synthesis of zinc oxide nanoparticles (ZnO NPs) using an aqueous extract of Cissus antractica. The green synthesis method offers great potential for developing new medications that enhance drug bioavailability. The current work highlighted the cytotoxicity, cell death, and routes of apoptosis in lung cancer cells (A549) and inflammatory effects through synthesizing zinc oxide nanoparticles (ZnO NPs) from the Cissus antractica plant using an eco-friendly methodology. UV–visible (UV-Vis) spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier-transform infrared spectroscopy (FT-IR), and energy-dispersive X-ray spectroscopy (EDS) were also used to characterize the synthesized ZnO nanoparticles. The average size of the NPs was 100 nm, and the NPs were crystalline in nature, as confirmed by FE-TEM and XRD analysis, respectively. In addition, the morphology of the nanoparticles analyzed by FE-TEM showed a spherical shape. The cell viability assay indicated that CA-ZnO NPs are non-toxic to normal cell lines at concentrations up to 20 µg/mL but showed significant toxicity in the A549 cell line. The nanoformulation also increased the ROS generation level in A549 lung cancer cells, and cellular apoptosis was confirmed via Hoechst and PI staining. The CA-ZnO NPs showed significant colony inhibition as well as cell migration ability that highlighted the CA-ZnO NPs as an anticancer agent. Additionally, this study demonstrated that NPs reduced the production of reactive oxygen species (ROS) and enhanced the expression of genes for BAX accumulation by releasing Cyto-c, but decreased Bcl-2 gene expression via the mitochondrial-mediated apoptosis pathway. In addition, the anti-inflammatory effect was also investigated; the CA-ZnO NPs showed significant NO inhibition ability with suppression of pro-inflammatory cytokines (TNF-α, iNOS, COX-2, IL-6, IL-8). In conclusion, Cissus antractica can be a source of significant Nano drugs with more advanced research in order to develop future anti-inflammatory and anticancer medications.

CRISPR- Cas9 Technology: Mechanism and Its Application in The Field of Entomology

The field of life science research has undergone a revolution thanks to the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and CRISPR-associated protein (CRISPR/Cas) system, which provides a multitude of opportunities for modifying, identifying, visualising, and annotating particular DNA or RNA sequences in diverse organisms. In this technique, foreign DNA pieces, known as spacers, are inserted into CRISPR cassettes. These spacers are then transcribed into CRISPR arrays and processed to produce guide RNA (gRNA). The Cas proteins that the CRISPR arrays encode serve as the enzymatic machinery required to obtain new spacers that specifically target invasive genetic elements. Several Cas proteins, such as Cas9, Cas12, Cas13, and Cas14, have been used to create novel tools for genome engineering due to their programmable sequence specificity. The ability to manipulate and edit nucleic acid sequences in living cells from a wide variety of organisms has been made possible by these Cas variants, which have greatly advanced genetic research and the CRISPR/Cas tool. The CRISPR Cas-9 technology has applications in many areas of entomology, including the genetics of honeybees and plants that produce insecticidal compounds. CRISPR/Cas9 technology has transformed entomology by providing precise tools for gene editing and genetic manipulation in insects. This has enabled advancements in fundamental research, disease vector control, and pest management, with the potential to reduce the environmental and economic impact of insect pests in agriculture and public health.