Flower-like CoNi layered double oxide (LDO) was successfully synthesized and applied to activate peroxymonosulfate (PMS) to degrade tetracycline (TC). CoNi LDO was rich in oxygen vacancies (OV) and had a high specific surface area. 30 mg/L TC was 100 % degraded in 60 min under the condition of 0.1 g/L CoNi LDO, 1.6 mM PMS, and unadjusted pH (5.45). A reaction mechanism dominated by OV was proposed in the TC removal system. Superoxide radical and singlet oxygen played major role in the reaction system, while sulfate radical and hydroxyl radical played minor role. The anti-interference ability of the CoNi LDO/PMS system to anions was relatively strong. At the same time, CoNi LDO showed high recovery efficiency. The degradation efficiency of TC was still higher than 92 % after five cycles. Through the detection of degradation intermediates, a possible degradation path was proposed. This study provided an efficient approach for TC degradation and provided a new clue for improving catalytic performance by introducing OV into the catalysts in PMS-based advanced oxidation processes systems.