Advances In Biosensors Research Articles

Advanced Biosensors: Electrochemical Impedance Study of Hybrid Materials Based on Layered Double Hydroxide/Trypsin

Advanced Biosensors: Electrochemical Impedance Study of Hybrid Materials Based on Layered Double Hydroxide/Trypsin

Programming the Dynamic Range of Nanochannel Biosensors for MicroRNA Detection Through Allosteric DNA Probes

AbstractSolid‐state nanochannel biosensors are extensively utilized for microRNA (miRNA) detection owing to their high sensitivity and rapid response. However, conventional nanochannel biosensors face limitations in their fixed dynamic range, restricting their versatility and efficacy. Herein, we introduce tunable triblock DNA probes with varying affinities for target miRNA to engineer solid‐state nanochannel biosensors capable of customizable dynamic range adjustment. The triblock DNA architecture comprises a poly‐adenine (polyA) block for adjustable surface density anchoring, alongside stem and loop blocks for modulating structural stability. Through systematic manipulation of these blocks, we demonstrate the ability to achieve diverse target binding affinities and detection limits, achieving an initial 81‐fold dynamic range. By combining probes with various affinities, we extend this dynamic range significantly to 10,900‐fold. Furthermore, by implementing a sequestration mechanism, the effective dynamic range of the nanochannel biosensor is narrowed to only a 3‐fold span of target concentrations. The customizable dynamic range of these advanced nanochannel biosensors makes them highly promising for a broad spectrum of biomedical and clinical applications.

Advancements in ascorbic acid quantification: from macroscopic analysis to miniaturized nano-sensing for quality control in food and beverage

Abstract Ascorbic acid (AA) or vitamin C, is vital in food safety, pharmaceutical and biomedical industries. This review discusses recent advancements in biosensors for AA detection. Traditional macro-analysis techniques such as high-performance liquids chromatography (HPLC), and flow injection analysis (FIA) provide accurate measurements but are costly and require specialized handling. Miniaturized nano-sensors are emerging as promising alternatives, offering advantages such as portability, cost effectiveness, and rapid response. This review explores the working principles of these nano-sensors, highlighting colorimetric, fluorometric and electrochemical detection methods. It also outlines the current AA biosensing landscape, detailing strategies to improve selectivity and specificity, including the choice of biorecognition elements and material selection for detection probe synthesis. Emerging nanomaterials and their applicability to support sensing activity are explored. This review serves as a valuable resource, suggesting future research directions to address existing challenges and further advance biosensor technology.

Programming the Dynamic Range of Nanochannel Biosensors for MicroRNA Detection through Allosteric DNA Probes.

Solid-state nanochannel biosensors are extensively utilized for microRNA detection owing to their high sensitivity and rapid response. However, conventional nanochannel biosensors face limitations in their fixed dynamic range, restricting their versatility and efficacy. Herein, we introduce tunable tri-block DNA probes with varying affinities for target miRNA to engineer solid-state nanochannel biosensors capable of programmable dynamic range adjustment. The tri-block DNA architecture comprises a poly-adenine (polyA) block for adjustable surface density anchoring, alongside stem and loop blocks for modulating structural stability. Through systematic manipulation of these blocks, we demonstrate the ability to achieve diverse target binding affinities and detection limits, achieving an initial 81-fold dynamic range. By combining probes with different affinities, we extend this dynamic range significantly to 10,900-fold. Furthermore, by implementing a sequestration mechanism, the effective dynamic range of the nanochannel biosensor was narrowed to only a 3-fold range of target concentrations. The customizable dynamic range of these advanced nanochannel biosensors makes them highly promising for a broad spectrum of biomedical and clinical applications.

Tunable Multi-Enzyme Activities of Platinum Nanoclusters for Enhanced Specificity and Sensitivity in Biosensing

Nanozymes have gained prominence for their utility in biosensing and disease diagnostics. However, challenges arise from complex sample matrices and nonspecific enzyme activities that contribute to false signals. This study introduces multifunctional platinum nanoclusters (Pt NCs) exhibiting peroxidase-like (POD-like), oxidase-like (OXD-like), and laccase-like activities tailored for enhanced biosensing capabilities. By adjusting pH, we optimized the conditions to achieve distinct POD-like and OXD-like responses, thereby reducing background signals and improving detection accuracy. The addition of ATP further amplified the POD-like activity while minimizing interference from OXD-like activity. This combined strategy substantially enhanced biomarker detection selectivity, demonstrated through glucose detection in human serum samples. Moreover, thiol inhibition of laccase-like activity in Pt NCs was leveraged for thiol-based antioxidant assessment, revealing their application in quantifying total antioxidant capacity (TAC) in human liver cancer cells, accounting for 44% of TAC. The Pt NCs demonstrated robust sensitivity and reusability, offering a novel multi-enzyme nanomaterial with potential for precise and interference-free biosensing applications. These findings contribute to the development of advanced nanozyme-based biosensors, addressing specificity regulation challenges and expanding their practical application in biosensing and disease diagnostics.

Rapid Assembly of Ultrafine Palladium Nanoparticle-Decorated HOF-101 Triggered by Guest Enzyme Encapsulation.

Rapid enzyme immobilization is essential for enzyme catalysis and sensing applications, yet constructing effective immobilization systems is challenging due to the need to balance enzyme activity with the properties of the surrounding framework. Herein, taking glucose oxidase (GOx) as a model, a rapid and straightforward approach was presented for synthesizing palladium nanoparticles (PdNPs)-decorated GOx encapsulated in HOF-101 nanocomposite materials (designated as PdNPs/GOx@HOF-101) through an in situ photoreduction and enzyme-triggering HOF-101 encapsulation. The enzyme's surface residues trigger the nucleation of HOF-101 around it through the hydrogen-bonded bio interface, completing the self-assembly of HOF-101 in 0.5 h. Furthermore, the biocomposites loaded with ultrafine PdNPs show satisfactory photoelectrochemical (PEC) properties. As a proof-of-concept, a PEC biosensor was constructed by utilizing PdNPs/GOx@HOF-101 as a photoactive probe, which can quickly and sensitively detect glucose and simultaneously remain stable within the circumstance of 30-60 °C and pH 4-8. These attributes pave the way for diverse applications, including improved enzyme immobilization techniques, advanced biosensors, and more efficient biocatalytic processes.

MNPs-aptazymes-PtNPs molecular motor biosensor mediated by circular cleavage reactions for the ultrasensitive detection of aflatoxin B1 in real samples

As a significant food safety risk factor linked to cancer, liver diseases, and kidney diseases, aflatoxin B1 (AFB1) necessitates rapid and ultrasensitive detection to mitigate its associated high morbidity and mortality rates. In this study, we developed an advanced biosensor for AFB1 detection by optimizing magnetic nanoparticles (MNPs), platinum nanoparticles (PtNPs), and aptazymes. The resulting MNPs-aptazymes-PtNPs molecular motor biosensor demonstrates promising capabilities for AFB1 detection. Initially, the specific binding between AFB1 and a split aptamer pair facilitates the conversion of intermolecular hybridization of DNAzyme strands into intramolecular hybridization on the MNPs surface. Then the catalytic strands of DNAzyme cleave substrate strands, resulting in PtNPs-containing fragments being released from the MNPs surface and enabling the molecular motor’s movement. The extensive cleavage of substrate strands by the long catalytic strands and the stepwise movement on MNPs surface are driven by the target-specific binding of AFB1. With high peroxidase-mimicking activity, the released PtNPs enable AFB1 detection after short incubation period. The MNPs-aptazymes-PtNPs molecular motor exhibits a sensitivity of 98.95 % and a specificity of 100 %, with a minimum detectable concentration of 300 fg/mL. This thermostabilized, cyclic, and enzyme-free nanomachine demonstrates broad applicability for the detection of various aflatoxins due to its target-empowered, sequence-independent mechanism, possesses excellent guarantee and supervision for enormous Ganoderma products in Shandong province, China.

DNA Origami Incorporated into Solid-State Nanopores Enables Enhanced Sensitivity for Precise Analysis of Protein Translocations.

The rapidly advancing field of nanotechnology is driving the development of precise sensing methods at the nanoscale, with solid-state nanopores emerging as promising tools for biomolecular sensing. This study investigates the increased sensitivity of solid-state nanopores achieved by integrating DNA origami structures, leading to the improved analysis of protein translocations. Using holo human serum transferrin (holo-hSTf) as a model protein, we compared hybrid nanopores incorporating DNA origami with open solid-state nanopores. Results show a significant enhancement in holo-hSTf detection sensitivity with DNA origami integration, suggesting a unique role of DNA interactions beyond confinement. This approach holds potential for ultrasensitive protein detection in biosensing applications, offering advancements in biomedical research and diagnostic tool development for diseases with low-abundance protein biomarkers. Further exploration of origami designs and nanopore configurations promises even greater sensitivity and versatility in the detection of a wider range of proteins, paving the way for advanced biosensing technologies.

Noninvasive Monitoring of Glycemia Level in Diabetic Patients by Wearable Advanced Biosensors.

We report on the possibility of noninvasive diabetes monitoring through continuous analysis of sweat. The prediction of the blood glucose level in diabetic patients is possible on the basis of their sweat glucose content due to the positive correlation discovered. The ratio between the blood glucose and sweat glucose concentrations for a certain diabetic subject is stable within weeks, excluding requirements for frequent blood probing. The glucose variations in sweat display allometric (non-linear) dependence on those in blood, allowing more precise blood glucose estimation. Selective (avoiding false-positive responses) and sensitive (sweat glucose is on average 30-50 times lower) detection is possible with biosensors based on the glucose oxidase enzyme coupled with a Prussian Blue transducer. Reliable glucose detection in just secreted sweat would allow noninvasive monitoring of the glycemia level in diabetic patients.

A label free ratiometric sensing of heparin using dynamic modulation of monomer–excimer equilibrium of coumarin 7

Heparin (Hp) is a multi-negatively charged polysaccharide with diverse physiological roles, particularly in regulating blood coagulation. Its clinical use as an anticoagulant requires a precise monitoring due to potential adverse effects at elevated levels. Therefore, there is a significant demand for an immediate, label-free, and user-friendly method to accurately quantify heparin (Hp). Herein we have explored a novel ratiometric sensing approach using coumarin-7 (C7) dye for precise Hp quantification. At pH 3, the protonated form of C7 undergoes aggregation upon interacting with polyanionic Hp via strong electrostatic forces. Consequently, there is alteration in the dynamics of the monomer-aggregate equilibrium for C7 in solution, resulting in the appearance of a new emission peak at ∼660 nm upon successive decline of the monomer peak at ∼512 nm. This leads to the generation of a ratiometric signal, which shows a nice linear response up to 6.8 μM of Hp to achieve limit of detection (LOD) of 89.6 nM for Hp in citrate buffer. Such ratiometric optical behaviour of C7 is not seen for any other interferants, which may be present in complex bio-matrices and thus, showcases a promising avenue for accurate and reliable Hp quantification even in complex biofluids. Additionally, the assembly C7 − Hp exhibits excellent responsiveness towards ionic strength of the medium, further extending its application towards the detection of an important bio-analyte, protamine (Pr). Importantly, this approach of ratiometric sensing strategy provides more accurate quantification due to its high reproducibility, minimal susceptibility to environmental effects, and self-calibration properties. The detailed spectroscopic analysis elucidates the dynamics of the C7 − Hp interaction, paving the way for advanced biosensing applications.

Enhanced performance of GO and RGO/Y2SiO5: Sm3+ nanocomposites for supercapacitors and biosensors

The increasing demand for high-performance materials in energy storage and biosensing applications highlights the need for ongoing research. Traditional materials often fail to meet the required standards for specific capacitance, energy density, and selectivity. Developing advanced nanocomposites, such as reduced graphene oxide (RGO) based Y2SiO5:Sm3+ (RYSOS), seeks to overcome these limitations, providing enhanced efficiency and stability. This study synthesized RYSOS nanocomposites for supercapacitor and biosensor applications, demonstrating superior performance over graphene oxide (GO) (GYSOS). RYSOS exhibited a higher specific capacitance of 474.81 Fg−1 compared to 396.68F−1 for GYSOS, and an energy density of 55.55 Wh/kg versus 32.89 Wh/kg. Additionally, RYSOS electrodes showed improved capacity retention at 87.83 % and coulombic efficiency at 91.54 % after 5000 cycles. In biosensing, RYSOS-modified carbon paste electrodes achieved excellent dopamine detection at pH 7.4, with a limit of detection (LOD) of 0.8579 µM and a limit of quantification (LOQ) of 2.859 µM. The developed electrode also demonstrated high selectivity for dopamine over uric acid and maintained 90 % stability over 10 cycles. These findings underscore the potential of RYSOS nanocomposites in enhancing the performance of advanced energy storage systems and biosensors, highlighting their effectiveness in specific capacitance, energy density, and selective biosensing capabilities.

Research on Drug Efficacy using a Terahertz Metasurface Microfluidic Biosensor Based on Fano Resonance Effect.

Advanced biosensors must exhibit high sensitivity, reliability, and convenience, making them suitable for detecting trace samples in biological or medical applications. Currently, biometric identification is the predominant method in clinical practice, but it is complex and time-consuming. In this study, we propose an optical metasurface utilizing the Fano resonance effect, which exhibits a sharp resonance with a transmittance of 32% at 0.65 THz. The resonance dip has a narrow bandwidth of 0.07 THz and a high Q-factor of 42. This resonance arises from the coupling of bright and dark modes, underpinned by the electromagnetic mechanism of Fano resonance. We integrated the metasurface into a microfluidic platform and fabricated low-temperature gallium arsenide photoconductive antennas (LT-GaAs-PCAs) on both sides of the microfluidics to efficiently generate and detect THz waves, significantly reducing the system's volume. The biosensor's detection limits for Escherichia coli (E. coli) and cefamandole nafate are 5 × 103 cells/mL and 5 μg/mL, respectively. Experimentally, when E. coli and cefamandole nafate solutions were sequentially injected into the microfluidic chip, a blue shift in the spectrum was observed. The sensor measured a 95.2% killing rate of E. coli by 40 μg/mL cefamandole nafate solution, with only a 3% deviation from biological experiments. Additionally, a timed killing experiment using 40 μg/mL cefamandole nafate on E. coli revealed a 93.7% killing rate within 3 min. This research presents a THz microfluidic biosensor with rapid detection, high sensitivity, and enhanced portability and integration, offering a promising approach for biomedical research, including antibiotic efficacy assessment and bacterial concentration monitoring.

Harnessing gold nanomaterials for advanced multicolor colorimetric biosensors in food hazards detection

Harnessing gold nanomaterials for advanced multicolor colorimetric biosensors in food hazards detection

Tetrodotoxin and the state-of-the-art progress of its associated analytical methods.

Tetrodotoxin (TTX), which is found in various marine organisms, including pufferfish, shellfish, shrimp, crab, marine gastropods, and gobies, is an effective marine toxin and the cause of many seafood poisoning incidents. Owing to its toxicity and threat to public health, the development of simple, rapid, and efficient analytical methods to detect TTX in various food matrices has garnered increasing interest worldwide. Herein, we reviewed the structure and properties, origin and sources, toxicity and poisoning, and relevant legislative measures of TTX. Additionally, we have mainly reviewed the state-of-the-art progress of analytical methods for TTX detection in the past five years, such as bioassays, immunoassays, instrumental analysis, and biosensors, and summarized their advantages and limitations. Furthermore, this review provides an in-depth discussion of the most advanced biosensors, including cell-based biosensors, immunosensors, and aptasensors. Overall, this study provides useful insights into the future development and wide application of biosensors for TTX detection.

Metal Halide Perovskite Nanocrystals-Intermediated Hydrogel for Boosting the Biosensing Performance.

Metal-halide perovskites have become attractive nanomaterials for advanced biosensors, yet the structural design remains challenging due to the trade-off between environmental stability and sensing sensitivity. Herein, a trinity strategy is proposed to address this issue by integrating Mn (II) substitution with CsPb2Cl5 inert shell and NH2-PEG-COOH coating for designing Mn2+-doped CsPbCl3/CsPb2Cl5 core/shell hetero perovskite nanocrystals (PMCP PNCs). The trinity strategy isolates the emissive Mn2+-doped CsPbCl3 core from water and the Mn2+ d-d transition generates photoluminescence with a long lifetime, endowing the NH2-PEG-COOH capped Mn2+-doped CsPbCl3/CsPb2Cl5 PNCs with robust water stability and oxygen-sensitive property. Given the structural integration, photoluminescent hydrogel biosensors are designed by embedding the PMCP PNCs into the hydrogel system to deliver on-site pesticide information on food products. Impressively, benefiting from the dual enzyme triggered-responsive property of PMCP PNCs, the hydrogel biosensor is endowed with ultra-high sensitivity toward chlorpyrifos pesticide at the nanogram per milliliter level. Such a robust PMCP PNCs-based hydrogel sensor can provide accurate pesticide information while guiding the construction of photoluminescent biosensors for upcoming on-site applications.

Bio-functionalized conductive poly(acrylic acid):poly(3,4-Ethylenedioxythiophene)-Prussian blue hybrid transducer for biosensors and bioelectronics interfaces

This study presents an innovative organic-inorganic hybrid transducer of poly(acrylic acid) (PAA), poly(3,4-ethylenedioxythiophene) (PEDOT) and Prussian blue (PB) nanocatalysts. The transducer demonstrated functionality conducive to enzyme conjugation and exhibited favorable electrochemical properties for biosensor signal transduction. Fabricated by a one-tube chemical redox method, the PAA:PEDOT-PB transducer showed long-term electrocatalytic and structural stability. The performance of the transducer was characterized by high transduction activity and low charge transfer resistance, particularly for H2O2 reduction. It achieves a linear detection range from 1.0 μM to 4.0 mM, with a sensitivity of 494 ± 10 μA mM−1 cm−1 and a LOD of 0.34 μM. The PAA:PEDOT-PB transducer featured a high density of carboxyl groups (DCOOH = 14.64 ± 0.05 μmol cm−2) that promoted the immobilization of the H2O2-dependent oxidase enzyme lactate oxidase (LOx) with an EDC/S–NHS coupling agent. The LOx-PAA:PEDOT-PB transducer was developed for lactate biosensing. The transducer provided high LOx-enzyme affinity (KMapp = 1.47 ± 0.05 mM), and a rapid response time (10 s) for lactate detection across a concentration range of 5.0 μM to 4.0 mM, showing a sensitivity of 223 ± 3 μA mM−1 cm−1 and an LOD of 1.45 μM. The LOx-PAA:PEDOT-PB transducer was integrated with a flexible screen-printed electrode, incorporating a wireless, battery-free near-field communication potentiostat module to measure lactate in artificial sweat on a skin model via smartphone. The PAA:PEDOT-PB transducer could enable connections with multiple bio-recognition molecules through polycarboxylic acid groups, providing potential avenues for the development of advanced biosensors.

Upcycling Compact Disks to Flexible Nanoporous Gold Electrodes for Nitrite Biosensing

Electrochemical biosensors are crucial in disease detection due to their simplicity, rapidity, and sensitivity. As a wearable or e-skin platform, electrochemical sensors are frequently restricted by gradual degradation, complexity, limited active surface area, arduous fabrication process, and high cost. Therefore, soft electrochemical biosensors that are mechanically biocompatible, easy to prototype, and robust for sensitive biosensing are highly sought. To mitigate such an issue, recordable compact disks (CDs) have been upcycled to advanced biosensors exhibiting flexible and disposable and being applicable to various biomedical applications, making them an economical and practical option. However, the limited surface area of the gold CDs electrode prevents optimal electrochemical catalytic performance. Nanostructure has been widely embedded in electrochemical biosensor construction to optimize electrode surface area and the number of active sites, which can contribute to improved detection sensitivity, selectivity, and electron transfer.Here, we aim to develop an advanced nanoporous gold film electrode (NPGFE) from CD for a simple, flexible, sensitive, and larger surface area electrode with reproducible areas. The fabrication and modification of gold CDs film were accomplished through two steps: 1) A mechanical cutter tailored the gold film after being transferred from CDs into a flexible substrate (Polyimide tape). Before placing the CDs film between the two laminating sheets, an aperture (1 mm diameter) was made in one of the laminating sheets to expose the tailored gold film electrode (GFE) and insulate the undesired area using a thermal laminator to ensure an adequate seal as a simple and low-cost approach; 2) The nanoporous gold (NPG) was electrochemically deposited onto the gold CDs film surface in gold (III) chloride trihydrate (HAuCl4·3H2O) and ammonium chloride (NH4Cl) solution at a potential of -4V for 100 s. Surface morphology characteristics and elemental analyses were investigated using scanning electron microscopy, while electrochemical studies were carried out in the CH Instruments potentiostat.The SEM images confirm a homogeneous distribution of NPG across the electrode’s surface. The energy-dispersive X-ray analyses exhibited the purity of the deposited NPG. The effective surface area was investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in 1 M KCl and 1 mM K3[Fe(CN)6] solution. From the slope of the Ip vs. v1/2 plot and the Randles-Sevcik equation, the active surface area of the NPGFE was significantly increased compared to the unmodified GFE. EIS was conducted to evaluate electron transfer resistance and obtain the semicircular diameter in Nyquist plots. In comparison to the unmodified GFE, the NPGFE showed a semicircle with a smaller diameter, indicating that the electron transfer resistance is minimized. The electrochemical characterization of the NPGFE was investigated by CV and amperometric techniques in 0.1 M PBS solution at ambient temperature (25°C) and pH 7.4. The NPGFE performance was studied at a fixed potential (0.77 V vs. Ag/AgCl) by analyzing the current response upon adding nitrite and anodic peak current at a potential range from 0.3 to 0.9 V (vs. Ag/AgCl). The calibration curve plot was linear over 0.5 μM to 9 mM with 20 nA·μM−1·cm−2 sensitivity and a low detection limit of 0.5 μM with a correlation coefficient (R2) of 0.99. The NPGFE's reproducibility reusability studies were conducted to evaluate electrode stability when prepared independently and utilized repeatedly under the same condition. Results demonstrate remarkable reproducibility and highly satisfactory reusability, making it suitable for practical application. The effect of pH on nitrite detection was investigated at different pH values. The anodic peak currents decreased and shifted to the low potential when the pH was increased from 5.0 to 10.0, indicating that protons were directly involved in the nitrite redox reaction. Also, the selectivity studies showed a significant increase in the current response when nitrite, ascorbic acid, and sodium sulfite were introduced to the solution, and there were no remarkable changes when other interferents were added. The long-term stability results showed that the NPGFE retains 99% of its initial current response to NO2 - after six months of storage, indicating satisfactory stability and long service life of NPGFE. Importantly, the sensing performance while stretching the NPGFE will be investigated to produce biocompatible relevant results.The flexible, nanoporous upcycled CDs electrode utilizing craft machines provide fast prototyping electrochemical biosensors for skin-interfaced bioelectronics. The nanoporous CD-based biosensor significantly improves electrocatalytic performance toward nitrite detection along with exceptional improvements in the cost-effective and sustainable fabrication process for flexible electronics. Future work relies on developing system-integrated biosensors based on systematic reliability and feasibility tests. Soft upcycled bioelectronics holds significant potential in advancing biomedical in situ real-time analysis in various platforms of flexible and nanostructured biosensors. Figure 1

Light-Controlled Regulation of Dual-Enzyme Properties in YbGd-Carbon Quantum Dots Nano-Hybrid for Advanced Biosensing.

Compared to nanozymes with single enzyme activity, those with multiple enzyme activities possess broader application potential due to their diversified enzymatic functionalities. However, the multienzyme nanozymes currently face challenges of interference among different enzymatic activities during practical applications. In this study, we report the synthesis of a light-responsive YbGd-carbon quantum dots nano-hybrid, termed YbGd-CDs, which exhibits controllable enzyme-mimicking activities. This light-responsive behavior enables selective control of the enzymatic activities. Under visible light irradiation, YbGd-CDs demonstrate robust oxidase-like activity. Conversely, under dark conditions, they primarily exhibit peroxidase-like activity. Leveraging the dual-enzyme-mimicking capabilities of YbGd-CDs, we developed colorimetric assays for sensitive detection of total antioxidant capacity (TAC) in both normal and cancer cells as well as d-amino acids in human saliva. This study not only advances the synthesis of carbon-based nanozymes but also highlights their potential in biosensing applications.

Optimization of graphene-based biosensor design for haemoglobin detection using the gradient boosting algorithm for behaviour prediction

This study presents an advanced biosensor based on metasurfaces for hemoglobin detection. The proposed sensor design integrates graphene with gold and silver, leveraging their exceptional optical properties and ability to support surface plasmon resonances. The metasurface-based architecture enhances interactions between the sensor and hemoglobin biomolecules, resulting in improved sensitivity and other performance parameters. Extensive optimization of the design parameters, including resonator dimensions and graphene chemical potential, was conducted to achieve an optimized sensor design. The sensor exhibits exceptional characteristics, including a peak sensitivity of 267 GHzRIU−1, a quality factor of 10.457, and a sensor resolution of 0.094, among other remarkable performance metrics. To streamline the optimization process and reduce computational complexity, the Gradient Boosting Algorithm (GBoost) is integrated into this study for behaviour prediction. The GBoost model demonstrates impressive performance, including an optimal coefficient of determination (R2) score of 1.0 for all cases considered, indicating perfect predictive accuracy within the model’s scope. These outstanding results suggest the significant potential of the proposed biosensor for rapid and precise blood testing, as well as monitoring medical conditions such as anaemia, by enabling early and accurate detection of hemoglobin levels. The sensor’s high-performance metrics, coupled with its simple design, represent a substantial advancement in the field of biosensing technology.

Recent Advancements in Biosensors for the Detection and Characterization of Amyloids: A Review.

Modern medicine has increased the human lifespan. However, with an increase in average lifespan risk of amyloidosis increases. Amyloidosis is a condition characterized by protein misfolding and aggregation. Early detection of amyloidosis is crucial, yet conventional diagnostic methods are costly and lack precision, necessitating innovative tools. This review explores recent advancements in diverse amyloid detection methodologies, highlighting the need for interdisciplinary research to develop a miniaturized electrochemical biosensor leveraging nanotechnology. However, the diagnostics industry faces obstacles such as skilled labor shortages, standardized selection processes, and concurrent multi-analyte identification challenges. Research efforts are focused on integrating electrochemical techniques into clinical applications and diagnostics, with the successful transition of miniaturized technologies from development to testing posing a significant hurdle. Label-free transduction techniques like voltammetry and electrochemical impedance spectroscopy (EIS) have gained traction due to their rapid, cost-effective, and user-friendly nature.