Upcycling Compact Disks to Flexible Nanoporous Gold Electrodes for Nitrite Biosensing

Abstract

Electrochemical biosensors are crucial in disease detection due to their simplicity, rapidity, and sensitivity. As a wearable or e-skin platform, electrochemical sensors are frequently restricted by gradual degradation, complexity, limited active surface area, arduous fabrication process, and high cost. Therefore, soft electrochemical biosensors that are mechanically biocompatible, easy to prototype, and robust for sensitive biosensing are highly sought. To mitigate such an issue, recordable compact disks (CDs) have been upcycled to advanced biosensors exhibiting flexible and disposable and being applicable to various biomedical applications, making them an economical and practical option. However, the limited surface area of the gold CDs electrode prevents optimal electrochemical catalytic performance. Nanostructure has been widely embedded in electrochemical biosensor construction to optimize electrode surface area and the number of active sites, which can contribute to improved detection sensitivity, selectivity, and electron transfer.Here, we aim to develop an advanced nanoporous gold film electrode (NPGFE) from CD for a simple, flexible, sensitive, and larger surface area electrode with reproducible areas. The fabrication and modification of gold CDs film were accomplished through two steps: 1) A mechanical cutter tailored the gold film after being transferred from CDs into a flexible substrate (Polyimide tape). Before placing the CDs film between the two laminating sheets, an aperture (1 mm diameter) was made in one of the laminating sheets to expose the tailored gold film electrode (GFE) and insulate the undesired area using a thermal laminator to ensure an adequate seal as a simple and low-cost approach; 2) The nanoporous gold (NPG) was electrochemically deposited onto the gold CDs film surface in gold (III) chloride trihydrate (HAuCl4·3H2O) and ammonium chloride (NH4Cl) solution at a potential of -4V for 100 s. Surface morphology characteristics and elemental analyses were investigated using scanning electron microscopy, while electrochemical studies were carried out in the CH Instruments potentiostat.The SEM images confirm a homogeneous distribution of NPG across the electrode’s surface. The energy-dispersive X-ray analyses exhibited the purity of the deposited NPG. The effective surface area was investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in 1 M KCl and 1 mM K3[Fe(CN)6] solution. From the slope of the Ip vs. v1/2 plot and the Randles-Sevcik equation, the active surface area of the NPGFE was significantly increased compared to the unmodified GFE. EIS was conducted to evaluate electron transfer resistance and obtain the semicircular diameter in Nyquist plots. In comparison to the unmodified GFE, the NPGFE showed a semicircle with a smaller diameter, indicating that the electron transfer resistance is minimized. The electrochemical characterization of the NPGFE was investigated by CV and amperometric techniques in 0.1 M PBS solution at ambient temperature (25°C) and pH 7.4. The NPGFE performance was studied at a fixed potential (0.77 V vs. Ag/AgCl) by analyzing the current response upon adding nitrite and anodic peak current at a potential range from 0.3 to 0.9 V (vs. Ag/AgCl). The calibration curve plot was linear over 0.5 μM to 9 mM with 20 nA·μM−1·cm−2 sensitivity and a low detection limit of 0.5 μM with a correlation coefficient (R2) of 0.99. The NPGFE's reproducibility reusability studies were conducted to evaluate electrode stability when prepared independently and utilized repeatedly under the same condition. Results demonstrate remarkable reproducibility and highly satisfactory reusability, making it suitable for practical application. The effect of pH on nitrite detection was investigated at different pH values. The anodic peak currents decreased and shifted to the low potential when the pH was increased from 5.0 to 10.0, indicating that protons were directly involved in the nitrite redox reaction. Also, the selectivity studies showed a significant increase in the current response when nitrite, ascorbic acid, and sodium sulfite were introduced to the solution, and there were no remarkable changes when other interferents were added. The long-term stability results showed that the NPGFE retains 99% of its initial current response to NO2 - after six months of storage, indicating satisfactory stability and long service life of NPGFE. Importantly, the sensing performance while stretching the NPGFE will be investigated to produce biocompatible relevant results.The flexible, nanoporous upcycled CDs electrode utilizing craft machines provide fast prototyping electrochemical biosensors for skin-interfaced bioelectronics. The nanoporous CD-based biosensor significantly improves electrocatalytic performance toward nitrite detection along with exceptional improvements in the cost-effective and sustainable fabrication process for flexible electronics. Future work relies on developing system-integrated biosensors based on systematic reliability and feasibility tests. Soft upcycled bioelectronics holds significant potential in advancing biomedical in situ real-time analysis in various platforms of flexible and nanostructured biosensors. Figure 1