Adhesion Area Research Articles

4D Force Detection of Cell Adhesion and Contractility.

Mechanical signals establish two-way communication between mammalian cells and their environment. Cells contacting a surface exert forces via contractility and transmit them at the areas of focal adhesions. External stimuli, such as compressive and pulling forces, typically affect the adhesion-free cell surface. Here, we demonstrate the collaborative employment of Fluidic Force Microscopy and confocal Traction Force Microscopy supported by the Cellogram solver to enable a powerful integrated force probing approach, where controlled vertical forces are applied to the free surface of individual cells, while the concomitant deformations are used to map their transmission to the substrate. Force transmission across human cells is measured with unprecedented temporal and spatial resolution, enabling the investigation of the cellular mechanisms involved in the adaptation, or maladaptation, to external mechanical stimuli. Altogether, the system enables facile and precise force interrogation of individual cells, with the capacity to perform population-based analysis.

Application of an Eco-Friendly Adhesive and Electrochemical Nanostructuring for Joining of Aluminum A1050 Plates

In adhesive joints used in several industrial applications, the adherends' bonding is made using an adhesive, which is usually an epoxy resin. However, since these adhesives are derived from petroleum fractions, they are harmful to the environment, due to the pollutants produced both during their manufacture and subsequent use. Thus, in recent years, effective steps have been made to replace these adhesives with ecological (green) ones. The present work focuses on the study of aluminum A1050 joints bonded with a green adhesive; the study also involves the electrochemical anodization method applied to adherends for nano-functionalization. The nanostructured aluminum adherends allow the formation of an expanded surface area for adhesion, compared to the non-anodized adherends. For comparison reasons, two different adhesives (Araldite LY1564 and Green Super Sap) were used. In addition, for the same reasons, both anodized and non-anodized aluminum adherends were joined with both types of adhesives. The lap joints were subsequently tested under both shear-tension and three-point bending conditions. The major findings were that aluminum A1050 anodization in all cases resulted in shear strength enhancement of the joints, while joints with both aluminum anodized and non-anodized adherends and bonded with the eco-friendly adhesive showed a superior shear behavior as compared to the respective joints bonded with Araldite adhesive.

Experimental and numerical study to determine the optimum type of composite material used in the repair of corroded and cracked Al A2017A aluminum plates

This study included two aspects - an experimental study and a numerical study – to determine which type of composite materials is more efficient in repairing corroded and cracked aluminum plates that have been repaired by the composite material technique. In the numerical study, the adhesive damage area theory was used to analyze the effect of corrosion and inclined crack behavior of Al 2017 A aluminum plate under thermo-mechanical loading. The effects of crack inclination and temperature change were highlighted as well as the effects of patch shape and type. In the experimental study, we prepared corroded sheets, repaired them with several types of composite materials, and then subjected them to tensile strength until wear. The results obtained either through a numerical or experimental study, proved that the boron/epoxy type composite is the most efficient in rust repair compared to the other types used in this study.

Frictional contact mechanics of asymmetric soft textures

Soft textures with asymmetric friction properties in shear against soft substrates have significant promise for many applications, particularly in the medical field. Here, we fabricate textures with directional friction properties and investigate the interaction between soft textures and soft substrates in shear. We experimentally determine that shear direction, texture stiffness, and presence of a lubricant affect coefficient of friction. Finite element models support experimental results and suggest underlying mechanisms. For soft, directional textures sheared against soft untextured substrates, unlubricated friction is primarily governed by adhesion and contact area while lubricated friction is primarily governed by a plowing effect of texture into the substrate.

Flexural strengthening of damaged steel beams with prestressed CFRP plates using a novel prestressing system

This study developed a novel prestressing system of the carbon fiber reinforced polymer (CFRP) plate to strengthen damaged steel beams, which reduced the operation space at the tensioning end, thereby extending the application range of prestressing system. Flexural tests were conducted to verify this system and investigate the effects of the adhesive layer, prestressing level, and CFRP plate area on the strengthening efficiency, and subsequently, a parametric analysis was carried out using finite element modeling to obtain more influencing rules of the unbonded CFRP plate-strengthened steel beams. The results indicated that satisfactory strengthening efficiency could be achieved by the novel prestressing system. The adhesive layer significantly affected the strain distributions of the steel–CFRP composite section, whereas it had a very slight effect on the flexural behavior of the steel beams, and the bonded and unbonded CFRP plates had a similar strengthening efficiency. Nonprestressed CFRP plate exhibited limited improvement in the flexural capacity and stiffness of the damaged steel beams under the normal service state; in contrast, the prestressed CFRP significantly increased the flexural behavior of the beams in the elastic and elastic–plastic stages owing to the high strength utilization of the CFRP plate. In addition, as the prestressing level increased, strengthening efficiency increased. Solely increasing the area or elastic modulus of the CFRP plate improved the strengthening efficiency slightly, whereas applying a prestress simultaneously clearly increases the strengthening efficiency. The strengthening efficiency increases with artificial notch depth.

Analysis of numeric results for analogue of Galin’s problem in curvilinear coordinates

Purpose. Studying the results obtained when applying the perturbation method to the individual contact problems solution. Considering the influence of non-ideal material properties of interaction bodies. Considering the contact area’s complex geometry influence. Methodology. Mathematical models of the interaction of an elastic plate with cylindrical anisotropy in the form of a curvilinear sector problems have been constructed and considered. In the process of interaction between the stamp and the plate, areas of slippage and adhesion occur. The original complex problems of the theory of elasticity in the process of applying the perturbation method are reduced to the sequential solution of the potential theory problems. Findings. An analytical solution to the interaction of an elastic anisotropic plate in the form of a curvilinear sector and a rigid stamp problem was obtained considering the existence of slippage and adhesion areas in the contact area. Laws of stress distribution under the stamp were found, as well as dependence of this distribution on the dimensions of the contact area. Originality. The perturbation method is convenient and expedient to use when solving mining mechanics problems. The study on the stress-strain state of an elastic anisotropic plate for the analogue of Galin’s problem was carried out, and the corresponding analytical solutions were obtained. The dependence of the coupling area size on the dimensions of the stamp and the opening angle of the sector, physical properties of the material is analyzed. Possible boundary transitions have been completed. Practical value. The proposed approach makes it possible to obtain analytical solutions to practically important problems in mining, to evaluate the stress-strain state of thick-walled structures with reinforcing elements, stamps, overlays. The results can be useful when designing mine workings.

Effects of reducing excess dental adhesive on bacterial adhesion in the bracket periphery

ObjectivesWhite spot lesions are one of the most common side effects of orthodontic therapy with a multibracket appliance and may indicate a preliminary stage of caries, also known as initial caries. Several approaches may be utilized to prevent these lesions, such as reducing bacterial adhesion in the area surrounding the bracket. This bacterial colonization can be adversely affected by a number of local characteristics. In this context, the effects of excess dental adhesive in the bracket periphery were investigated by comparing a conventional bracket system with the APC flash-free bracket system.Materials and methodsBoth bracket systems were applied to 24 extracted human premolars, and bacterial adhesion with Streptoccocus sobrinus (S. sobrinus) was performed for 24 h, 48 h, 7 d, and 14 d. After incubation, bacterial colonization was examined in specific areas by electron microscopy.ResultsOverall, significantly fewer bacterial colonies were found in the adhesive area around the APC flash-free brackets (n = 507 ± 13 bacteria) than the conventionally bonded bracket systems (n = 850 ± 56 bacteria). This is a significant difference (**p = 0.004). However, APC flash-free brackets tend to create marginal gaps with more bacterial adhesion in this area than conventional bracket systems (n = 265 ± 31 bacteria). This bacterial accumulation in the marginal-gap area is also significant (*p = 0.029).ConclusionA smooth adhesive surface with minimal adhesive excess is beneficial for reducing bacterial adhesion but also poses a risk of marginal gap formation with subsequent bacterial colonization, which can potentially trigger carious lesions.Clinical relevanceTo reduce bacterial adhesion, the APC flash-free bracket adhesive system with low adhesive excess might be beneficial.APC flash-free brackets reduce the bacterial colonization in the bracket environment.A lower number of bacteria can minimize white spot lesions in the bracket environment.APC flash-free brackets tend to form marginal gaps between the bracket adhesive and the tooth.

Dendrimer and dendrimer gel-derived drug delivery systems: Breaking bottlenecks of topical administration of glaucoma medications.

Due to high structural flexibility, multidrug carrying capability, and tunable size, dendrimers have been used as suitable carriers for ophthalmic drug delivery. Drug molecules can be either encapsulated or chemically coupled to dendrimers. The nanoscopic size, spheroidal shape, and cationic surface of polyamidoamine (PAMAM) dendrimers promote their interaction with the cornea and result in prolonged precorneal retention. Dendrimers could be further cross-linked to produce three-dimensional hydrogel networks or dendrimer hydrogels (DH). The properties of the DH can be readily adjusted to maintain both fluidity and adhesiveness, making them suitable for developing topical ocular drug formulations. Micro-/nano-sized DHs, that is, dendrimer micro-/nano-gels, have unique properties such as ease of administration, large specific surface area for adhesion, and drug targeting functionalities, making them attractive for ophthalmic drug delivery. This perspective reports advances in PAMAM dendrimer based drug delivery systems including drug conjugates and micro- and nano-gels to enhance and sustain the delivery of multiple anti-glaucoma drugs, Dendrimer and dendrimer gel-derived drug delivery systems hold great potential as multifunctional topical drug delivery systems for the eye.

Impact of adhesive area on cellular traction force and spread area.

Cells integrate endogenous and exogenous mechanical forces to sense and respond to environmental signals. In particular, cell-generated microscale traction forces regulate cellular functions and impact macroscale tissue function and development. Many groups have developed tools for measuring cellular traction forces, including microfabricated post array detectors (mPADs). mPADs are a powerful tool that provides direct traction force measurements through imaging post deflections and utilizing Bernoulli-Euler beam theory. In this technical note, we investigated how mPADs presenting two different top surface areas but similar effective stiffness influence cellular spread area and traction forces for murine embryonic fibroblasts and human mesenchymal stromal cells. When focal adhesion size was restricted via mPAD top surface area, we observed a decrease in both cell spread area and cell traction forces as the mPAD top surface area decreased, but the traction force-cell area linear relationship was maintained, which is indicative of cell contractility. We conclude that the mPAD top surface area is an important parameter to consider when utilizing mPADs to measure cellular traction forces. Furthermore, the slope of the traction force-cell area linear relationship provides a useful metric to characterize cell contractility on mPADs.

Multistage Gradient Bioinspired Riblets for Synergistic Drag Reduction and Efficient Antifouling.

Shark skin-inspired riblets have represented the tremendous potential for drag reduction (DR) and antifouling in submarine, ship, and so on. Most studies simplified the complex denticle embedded in the shark skin into the single-stage riblet with uniform parameters, ignoring the influence of riblet height gradient and material deformation on DR and antifouling. In the present study, flexible multistage gradient riblets (MSGRs) with varied heights were proposed, and their DR and antifouling effects were investigated by the experiment and numerical simulation. The experimental results showed that the maximum DR rate of flexible MSGRs with an elastic modulus of 4.592 MPa could reach 16.8% at a flow velocity of 0.5 m/s. Moreover, the dynamic adhesion measurement indicated a reduction by 69.6% of the adhesion area of Chlorella vulgaris on the flexible MSGR surface. The results identified that flexible MSGRs with low surface energy could generate steady high- and low-velocity streaks and alter the flow state of the fluid, thus lessening the average velocity gradient near the wall and the adhering selectivity of pollutants in riblet and achieving synergistic DR and efficient antifouling. Taken together, the proposed flexible MSGR surface holds promise for reducing surface friction and inhibiting particle attachment in engineering applications.

Development of centrifugal coating device to load CuZnZr/γ-Al2O3 catalyst on porous substrates for methanol steam reforming

The porous foam material has been attracted to be used as the catalyst coating substrate for microreactors due to its generally excellent heat and mass transfer characteristics. Preparing uniform catalyst coating onto porous foam can increase the adhesion area between the coating and substrate to reduce spallation and extend the service life of microreactors. Herein, we developed a highly automatic centrifugal coating device to fabricate relatively uniform catalyst coating onto porous foam structures. The structural and hardware components of the automatic centrifugal coating device are introduced, and an active disturbance rejection control (ADRC) algorithm is adopted to accurately adjust the centrifugal speed with an error of <1 % to ensure the relative uniformity of catalyst coating. By using the developed device, the CuZnZr/γ-Al2O3 catalyst coating can be successfully loaded onto three types of foam substrates (Cu, α-SiC/α-Al2O3, α-Al2O3) with high relative uniformity. The spallation resistance was examined by thermal shock and high-speed steam scouring tests. After that, the porous foam substrate with coated catalyst was used in microreactors for methanol steam reforming, results showed that the conversion rate of CuZnZr/γ-Al2O3/Cu foam structured catalyst can reach 90.7 % and hydrogen flow rate is 296.18 mL/min at 300 °C. This study demonstrates our developed centrifugal coating device has the potential for industrial application of structured catalyst preparation.

Sizing up spotted lanternfly nymphs for instar determination and growth allometry.

A major ongoing research effort seeks to understand the behavior, ecology and control of the spotted lanternfly (SLF) (Lycorma delicatula), a highly invasive pest in the U.S. and South Korea. These insects undergo four nymphal stages (instars) before reaching adulthood, and appear to shift host plant preferences, feeding, dispersal and survival patterns, anti-predator behaviors, and response to traps and chemical controls with each stage. However, categorizing SLF life stage is challenging for the first three instars, which have the same coloration and shape. Here we present a dataset of body mass and length for SLF nymphs throughout two growing seasons and compare our results with previously-published ranges of instar body lengths. An analysis using two clustering methods revealed that 1st-3rd instar body mass and length fell into distinct clusters consistently between years, supporting using these metrics to stage nymphs during a single growing season. The length ranges for 2nd-4th instars agreed between years in our study, but differed from those reported by earlier studies for diverse locations, indicating that it is important to obtain these metrics relevant to a study's region for most accurate staging. We also used these data to explore the scaling of SLF instar bodies during growth. SLF nymph body mass scaled with body length varied between isometry (constant shape) and growing somewhat faster than predicted by isometry in the two years studied. Using previously published data, we also found that SLF nymph adhesive footpad area varies in direct proportion to weight, suggesting that footpad adhesion is independent of nymphal stage, while their tarsal claws display positive allometry and hence disproportionately increasing grasp (mechanical adhesion). By contrast, mouthpart dimensions are weakly correlated with body length, consistent with predictions that these features should reflect preferred host plant characteristics rather than body size. We recommend future studies use the body mass vs length growth curve as a fitness benchmark to study how SLF instar development depends on factors such as hatch date, host plant, temperature, and geographic location, to further understanding of life history patterns that help prevent further spread of this invasive insect.

The Preparation of Photocatalytic Porous Magnesium Oxychloride Cement-Based Materials and Its De-NOx Performance

Porous magnesium oxychloride cement (PMOC) has a high specific surface area formed by interlocking whiskers, which can be used as a promising photocatalyst substrate for the photocatalytic removal of atmospheric pollutants. In this paper, magnesium oxychloride cement (MOC) was used as matrix and TiO2 as catalyst to prepare MOC blocks. Plant-based protein was used as a foaming agent to form the layered porous structure suitable for supporting TiO2 particles, which effectively increased the surface area of light radiation and TiO2 adhesion area in photocatalytic porous magnesium oxychloride cement (PPMOC). It was found that the addition of the foaming agent can increase the adsorption capacity of MOC to TiO2. The vacuum-immersion loading method can effectively support TiO2 on the surface of PMOC. The photocatalytic performance of PPMOC can be improved by multiple loading, while higher porosity of PMOC would reduce the loading surface of matrix to TiO2 particles, which might decrease the photocatalytic efficiency. As can be observed in PPMOC specimens, when the porosity of PPMOC is less than 60%, increasing the porosity can improve the photocatalytic efficiency, while when the porosity is higher than 60%, increasing the porosity decreased the photocatalytic efficiency due to the reduction of the loading surface. The excellent nitrate selectivity of PPMOC also shows good application potential in the field of catalytic degradation of nitrogen oxides.

Extracellular Cues Govern Shape and Cytoskeletal Organization in Giant Unilamellar Lipid Vesicles.

Spontaneous and induced front-rear polarization and a subsequent asymmetric actin cytoskeleton is a crucial event leading to cell migration, a key process involved in a variety of physiological and pathological conditions such as tissue development, wound healing, and cancer. Migration of adherent cells relies on the balance between adhesion to the underlying matrix and cytoskeleton-driven front protrusion and rear retraction. A current challenge is to uncouple the effect of adhesion and shape from the contribution of the cytoskeleton in regulating the onset of front-rear polarization. Here, we present a minimal model system that introduces an asymmetric actin cytoskeleton in synthetic cells, which are resembled by giant unilamellar lipid vesicles (GUVs) adhering onto symmetric and asymmetric micropatterned surfaces. Surface micropatterning of streptavidin-coated regions with varying adhesion shape and area was achieved by maskless UV photopatterning. To further study the effects of GUV shape on the cytoskeletal organization, actin filaments were polymerized together with bundling proteins inside the GUVs. The micropatterns induce synthetic cell deformation upon adhesion to the surface, with the cell shape adapting to the pattern shape and size. As expected, asymmetric patterns induce an asymmetric deformation in adherent synthetic cells. Actin filaments orient along the long axis of the deformed GUV, when having a length similar to the size of the major axis, whereas short filaments exhibit random orientation. With this bottom-up approach we have laid the first steps to identify the relationship between cell front-rear polarization and cytoskeleton organization in the future. Such a minimal system will allow us to further study the major components needed to create a polarized cytoskeleton at the onset of migration.

Effects of Various Cell Surface Engineering Reactions on the Biological Behavior of Mammalian Cells.

Cell surface engineering technologies can regulate cell function and behavior by modifying the cell surface. Previous studies have mainly focused on investigating the effects of cell surface engineering reactions and materials on cell activity. However, they do not comprehensively analyze other cellular processes. This study exploits covalent bonding, hydrophobic interactions, and electrostatic interactions to modify the macromolecules succinimide ester-methoxy polyethylene glycol (NHS-mPEG), distearoyl phosphoethanolamine-methoxy polyethylene glycol (DSPE-mPEG), and poly-L-lysine (PLL), respectively, on the cell surface. This work systematically investigates the effects of the three surface engineering reactions on the behavior of human umbilical vein endothelial cells (HUVECs) and human skin fibroblasts, including viability, growth, proliferation, cell cycle, adhesion, and migration. The results reveals that the PLL modification method notably affects cell viability and G2/M arrest and has a short modification duration. However, the DSPE-mPEG and NHS-mPEG modification methods have little effect on cell viability and proliferation but have a prolonged modification duration. Moreover, the DSPE-mPEG modification method highly affects cell adherence. Further, the NHS-mPEG modification method can significantly improve the migration ability of HUVECs by reducing the area of focal adhesions. The findings of this study will contribute to the application of cell surface engineering technology in the biomedical field.

Structure-Based Design of Dual Bactericidal and Bacteria-Releasing Nanosurfaces.

Here, we report synergistic nanostructured surfaces combining bactericidal and bacteria-releasing properties. A polystyrene-block-poly(methyl methacrylate) (PS-block-PMMA) diblock copolymer is used to fabricate vertically oriented cylindrical PS structures ("PS nanopillars") on silicon substrates. The results demonstrate that the PS nanopillars (with a height of about 10 nm, size of about 50 nm, and spacing of about 70 nm) exhibit highly effective bactericidal and bacteria-releasing properties ("dual properties") against Escherichia coli for at least 36 h of immersion in an E. coli solution. Interestingly, the PS nanopillars coated with a thin layer (≈3 nm thick) of titanium oxide (TiO2) ("TiO2 nanopillars") show much improved dual properties against E. coli (a Gram-negative bacterium) compared to the PS nanopillars. Moreover, the dual properties emerge against Listeria monocytogenes (a Gram-positive bacterium). To understand the mechanisms underlying the multifaceted property of the nanopillars, coarse-grained molecular dynamics (MD) simulations of a lipid bilayer (as a simplified model for E. coli) in contact with a substrate containing hexagonally packed hydrophilic nanopillars were performed. The MD results demonstrate that when the bacterium-substrate interaction is strong, the lipid heads adsorb onto the nanopillar surfaces, conforming the shape of a lipid bilayer to the structure/curvature of nanopillars and generating high stress concentrations within the membrane (i.e., the driving force for rupture) at the edge of the nanopillars. Membrane rupture begins with the formation of pores between nanopillars (i.e., bactericidal activity) and ultimately leads to the membrane withdrawal from the nanopillar surface (i.e., bacteria-releasing activity). In the case of Gram-positive bacteria, the adhesion area to the pillar surface is limited due to the inherent stiffness of the bacteria, creating higher stress concentrations within a bacterial cell wall. The present study provides insight into the mechanism underlying the "adhesion-mediated" multifaceted property of nanosurfaces, which is crucial for the development of next-generation antibacterial surface coatings for relevant medical applications.

Bionic artificial penile Tunica albuginea

Bionic artificial penile Tunica albuginea

Safety of mesh with fluoropolymer coating during intra-abdominal placement in large animals: results of the pilot study

When performing laparoscopic intraperitoneal hernioplasty (IPOM), endoprostheses made of fluoropolymers are often used. However, there is no data in the literature on the intra-abdominal use of inexpensive polyester prostheses with a fluoropolymer coating compared to composite implants. Thus, the aim of the pilot study was a preliminary assessment of the safety profile of FTOREX mesh endoprostheses during intra-abdominal placement in large animals. 6 endoprostheses were installed laparoscopically intraperitoneally in each of the 3 pigs: 1) FTOREX; 2) FTOREX with a layer of carboxymethylcellulose; 3) REPEREN-16-2; 4) SYMBOTEX; 5) VENTRALIGHT ST; 6) decellularized pork peritoneum. Fixation was performed with a herniator, transfascial sutures were not used. Relaparoscopy was performed after 45 days, and withdrawal from the experiment was performed after 90 days. Performance characteristics, signs of deformation and retraction, parameters of spike formation were evaluated. All the animals survived, no complications were observed. There were no clinical manifestations or behavioral reactions indicating the presence of adhesions. The most convenient to use were the SYMBOTEX and FTOREX implants (5.0 points each). By the end of the experiment, deformation and retraction were noted in both variants of the FTOREX implants and the REPEREN prosthesis. These changes were completely absent only when using the SYMBOTEX endoprosthesis. According to the number of implants with adhesions, by the end of the observation, both variants of FTOREX prostheses occupied an intermediate position between the Reference (the worst indicator) and VENTRALIGHT ST (the best indicator). However, both FTOREX endoprostheses showed the best performance among all implants in the integral assessment of adhesions, as well as in terms of parameters such as the area and appearance of adhesions, and in terms of the strength of the joints, they were second only to the VENTRALIGHT ST endoprosthesis (0.67 vs. 0.5 points). During the study, there was no reliable dependence of deformation, retraction and adhesion formation indicators on the type of implant. The results of the pilot study showed that all the implants used did not cause any clinically significant adverse reactions or complications. FTOREX endoprostheses with their intraperitoneal installation have anti-adhesive properties that are not inferior to VENTRALIGHT ST or SYMBOTEX composite implants. However, having less rigidity, they are more often deformed and subjected to retraction.

Lumbrokinase, a Fibrinolytic Enzyme, Prevents Intra-Abdominal Adhesion by Inhibiting the Migrative and Adhesive Activities of Fibroblast via Attenuation of the AP-1/ICAM-1 Signaling Pathway.

Intra-abdominal adhesion is a complication following abdominal surgery caused by the suppression of fibrinolytic activity and aggravated fibroblast invasion of the injured area, which may lead to chronic illnesses such as chronic pain, intestinal obstruction, and female infertility. This study hypothesized that lumbrokinase, a fibrinolytic enzyme extracted from the earthworm, supports the wound healing process. Therefore, we assessed the effect of lumbrokinase on intra-abdominal adhesion. Lumbrokinase treatment significantly decreased the severity and the area of intra-abdominal adhesion in vivo in a dose-dependent manner compared with the controls (untreated and hyaluronate-treated). Lumbrokinase-associated adverse effects were not observed. Immunohistochemical analysis of adhesion tissues revealed a loosened adhesive band between tissues, coupled with significantly decreased peritoneal thickening in the lumbrokinase-treated group versus the control group. Three-dimensional spheroid, MTT, and scratch wound migration assays using the IMR-90 human fibroblast cell line demonstrated that lumbrokinase significantly attenuated the migration and adhesive activity of fibroblasts without compromising cell proliferation. The luciferase assay and western blot analysis showed that lumbrokinase inhibited the AP-1/ICAM-1 cell adhesion signaling pathway. Therefore, lumbrokinase decreases intra-abdominal adhesion and peritoneal thickening by augmenting fibrinolytic action and inhibiting fibroblast migration and adhesive activity via attenuation of the AP-1/ICAM-1 signaling pathway. Lumbrokinase is thus a promising agent to prevent intra-abdominal adhesion.

STUDY OF THE MATERIAL PROPERTIES INFLUENCE AND CONTACT CONDITIONS ON THE STRESS-STRAIN STATE DURING THE INTERACTION OF A STAMP AND A PLATE

The article examines the results obtained when applying the perturbation method to the solution of individual contact problems.Attention is paid to the influence of non-ideal properties of materials of interaction bodies. The influence of the complex geometry of the contact zone is also taken into account. In the article, mathematical models of problems on the interaction of an elastic orthotropic semi-infinite plate with cylindrical anisotropy in the form of a curvilinear sector are constructed and considered. In the process of interaction between the stamp and the plate, there are areas of sliding and adhesion. The original problems of the theory of elasticity, difficult in their initial formulation, in the process of applying the perturbation method are reduced to the sequential solution of simpler problems of the theory of functions of a complex variable. Analytical solutions to the problem of the interaction of an elastic anisotropic semi-infinite plate in the form of a curvilinear sector and a rigid stamp were obtained, taking into account the presence of areas of sliding and adhesion in the contact zone. As significant results obtained after solving the formulated contact problems, it is possible to indicate the found regularities of stress distribution under the stamp, as well as the dependence of this distribution on the dimensions of the contact area. An analysis of the dependence of the size of the coupling zone on the stiffness characteristics of the sector was also carried out, taking into account different materials (for the sector), the opening angle of the sector and the dimensions of the stamp. In order to further develop the method and prove the convenience of applying the perturbation method in complex mixed boundary value problems, a study of the stress-strain state of an elastic anisotropic plate for an analogue of the Galin problem was carried out, while cases for different plate materials were considered. Corresponding analytical solutions were also obtained. The dependence of the size of the adhesion area on the dimensions of the stamp and the opening angle of the sector, as well as on the physical properties of the material of the sector, was analyzed. Possible boundary transitions are performed in the work and a comparison with known scientific results is made. The approach proposed by the authors makes it possible to obtain analytical solutions to practically important problems of contact interaction, to carry out possible assessments of the stress-strain state of thick-walled structures with reinforcing elements, stamps, overlays, etc. The results may also be useful. when designing mining works. At the same time, such important issues as the peculiarities of the physical properties of the materials of the interacting bodies, the complex geometry of the contact zone, the occurrence of areas of sliding and adhesion in the contact zone are taken into account.