Serum Adenosine Deaminase Activity Research Articles

Oxidative stress and its relationship with adenosine deaminase activity in various stages of breast cancer.

Reactive oxygen species (ROS) cause damage to the DNA producing mutations and formation of tumours such as carcinoma of breast. Tumour cells are known to produce ROS at a greater pace than the non-transformed cells. The increased production of reactive oxygen species causes oxidative stress leading to cell proliferation and hence increased inflammatory conditions. The present study was aimed to investigate the role of oxidative stress in the pathogenesis of breast cancer. Females suffering from breast cancer had significantly decreased Superoxide dismutase (SOD) and reduced glutathione (GSH) levels in comparison to normal females. The compromised antioxidant defence system produces the oxidative stress which in turn creates the inflammatory response shown by concomitant increased adenosine deaminase (ADA) activity in female patients. ADA diminishes the protective molecule adenosine. There were significant variations (p<0.01) in ADA activity with different clinical stages (stage 1-4) of breast cancer suggesting thereby that estimation of ADA activity can be used as a diagnostic tool to detect the stage of cancer along with cytological studies. Mastectomy was performed and post-operatively serum SOD and ADA activity and plasma GSH levels were estimated. There was a statistically significant increase in activity of SOD and levels of GSH while serum ADA activity decreased significantly, suggesting thereby that oxidative stress is responsible for increased cell proliferation and hence the inflammatory conditions in CA breast that got ameliorated post-operatively.

Evaluation of serum adenosine deaminase activity in acute lymphoid leukemia in Erbil city

Background and objectives: Adenosine deaminase (ADA), is an enzyme that catalyzes hydrolytic deamination of either adenosine or deoxy adenosine to produce inosine and deoxy inosine respectively. The aim of the present study is to evaluate the serum ADA activity in acute lymphoid leukemia. Method: A prospective study was carried out from January to June 2011 by the clinical biochemistry department in College of Medicine-Hawler Medical University on (30) patients with Acute lymphoid leukemia (group 1), and (30) healthy individuals, (group 2). Results: The mean value of serum ADA activity was significantly lower in Acute lymphoid leukemia (group 1), than those of healthy individuals, (group 2) (p&lt;0.01), and both ions&nbsp;&nbsp; Na+ and K+ acted as an activators for Serum ADA activity. Conclusion: Based on the findings, it can be concluded that in acute lymphoid leukemia the activity of Serum ADA was decreased due to decline in the immunity.

Adenosine deaminase activity in patients with ovarian neoplasms

The aim of this study was to investigate the serum and peritoneal fluid adenosine deaminase (ADA) activity in patients with benign and malignant ovarian neoplasms. This is a prospective cross-sectional study performed in Cukurova University, Department of Gynecologic Oncology. Eighty-four patients with ovarian mass were included in this study within 13 months. The levels of serum and peritoneal fluid ADA levels were measured and compared in patients with benign and malignant ovarian neoplasms and also low- and high-grade malignant tumors. Serum and peritoneal fluid ADA levels were found to be significantly higher in patients with ovarian cancers as compared with benign ovarian tumors (p = 0.001). Additionally, ADA levels were found to be significantly different according to the histopathological subtypes and grade of ovarian cancers. However, there was no significant difference for ADA levels between the benign and low-grade malignant tumors. There was an important correlation between the peritoneal fluid and serum ADA levels. Serum and peritoneal fluid ADA levels were found to be higher in malignant ovarian neoplasms. This finding may be useful to understand the biochemically characteristics of malignant ovarian tumors and ADA may be a useful biomarker in diagnosis and management of ovarian tumors.

Influence of Hyperthermic Environmental Temperature on Adenosine Deaminase Activity in Serum and Lymphoid Organs of Wistar Rats

Aim: To analyze the influence of the hyperthermic environmental temperature (35 ± 1°C) on the adenosine deaminase activity (ADA) in tissues rich with lymphoid cells in Wistar rats. Material and Methods: The activity of ADA was examined in thymus, spleen, Payer’s patches, mesenteric lymph nodes and serum. The experimental animals (n = 11 male rats) were exposed on hyperthermic environment (35 ± 1°C) and relative air humidity of 20 - 30% within a period of 30 days. The control group of animals (n=10) were kept on room temperature (18 - 22°C). Results: The body weight was decreased, spleen mass, and the thymus mass were reduced. Activity of ADA in the thymus was significantly increased from 10.58 ± 1.57 U/g in the control group of rats to 13.38 ± 2.7 U/g in rats exposed on 35 ± 1°C (p<0.01), and in the spleen it raised from 5.17 ± 3.29 E/g to 7.8 ± 1.97 U/g (p<0.05). The ADA activity in the other lymphoid organs and serum showed no statistically significant changes between the two groups of examined rats. Conclusions: Influence of hyperthermic environment (35 ± 1°C, 30 days) provokes decrease in body weight, spleen and thymus relative mass, increase of the enzyme activity of ADA in thymus and spleen of rats, but there are no changes in the other lymphoid organs and tissues.

Sputum adenosine deaminase and alkaline phosphatase activity in pulmonary tuberculosis

Objective: The aim was to determine adenosine deaminase (ADA) and alkaline phosphatase (ALP) activity in sputum for the diagnosis of pulmonary tuberculosis.Methods: This study comprised of 64 subjects (33 pulmonary tuberculosis and 31 lung cancer). The ADA and ALP activity was measured in sputum and serum.Results: The mean ADA activity in sputum, serum and mean sputum/serum ADA ratio was 205.20 IU/L, 42.32 IU/L and 4.90 in pulmonary tuberculosis and 127.74 IU/L, 29.90 IU/L and 4.27 in lung cancer subjects respectively. The mean ALP activity in sputum, serum and mean sputum/serum ALP ratio was 461.34 IU/L, 159.24 IU/L and 2.90 in pulmonary tuberculosis and 226.64 IU/L, 119.87 IU/L and 1.99 in lung cancer subjects respectively.Conclusion: The mean ADA and ALP activity was significantly increased in pulmonary tuberculosis as compared to lung cancer subjects and hence may be a useful tool for the diagnosis of pulmonary tuberculosis.

Serum adenosine deaminase, catalase and carbonic anhydrase activities in patients with bladder cancer

OBJECTIVES:The relationship between adenosine deaminase and various cancers has been investigated in several studies. However, serum adenosine deaminase activity and carbonic anhydrase and catalase activities in patients with bladder cancer have not previously been reported. Therefore, the aim of this study was to measure serum adenosine deaminase, carbonic anhydrase and catalase activities in patients with bladder cancer.MATERIALS AND METHODS:Forty patients with bladder cancer and 30 healthy controls were enrolled in the study. Serum adenosine deaminase, carbonic anhydrase and catalase activities were measured spectrophotometrically.RESULTS:Serum adenosine deaminase, carbonic anhydrase and catalase activities were significantly higher in patients with bladder cancer than controls (all significant, p<0.001).CONCLUSIONS:These markers might be a potentially important finding as an additional diagnostic biochemical tool for bladder cancer.

Adenosine Deaminase Activity in Serum and Lymphocytes of Rats Infected with Sporothrix schenckii

Sporotrichosis is a fungal infection of subcutaneous or chronic evolution, inflammatory lesions characterized by their pyogranulomatous aspect, caused by the dimorphic fungus Sporothrix schenckii. Adenosine deaminase (ADA) is a "key" enzyme in the purine metabolism, promoting the deamination of adenosine, an important anti-inflammatory molecule. The increase in ADA activity has been demonstrated in several inflammatory conditions; however, there are no data in the literature associated with this fungal infection. The objective of this study was to evaluate the activity of serum ADA (S-ADA) and lymphocytes (L-ADA) of rats infected with S. schenckii. We used seventy-eight rats divided into two groups. In the first experiment, rats were infected subcutaneously and in the second experiment, infected intraperitoneally. Blood samples for hematologic evaluation and activities of S-ADA and L-ADA were performed at days 15, 30, and 40 post-infection (PI) to assess disease progression. In the second experiment, it was observed an acute decrease in activity of S-ADA and L-ADA (P<0.05), suggesting a compensatory mechanism in an attempt to protect the host from excessive tissue damage. With chronicity of disease the rats in the first and second experiment at 30days PI showed an increased activity of L-ADA (P<0.05), promoting an inflammatory response in an attempt to combat the spread of the agent. Thus, it is suggested that infection with S. schenckii alters the activities of S-ADA in experimentally infected rats, demonstrating the involvement of this enzyme in the pathogenesis of sporotrichosis.

Elevated adenosine deaminase activity in overweightand obese Indian subjects

Objective: To determine adenosine deaminase activity in overweight and obese Indian subjects.Methods: This study comprised of 100 subjects. The body mass index (BMI) of subjects was calculated and adenosine deaminase activity was determined in their fasting blood sample. The study was divided into three groups depending upon BMI: Control (n = 40, BMI < 25 kg/m2), Overweight (n = 30, BMI 25–29.9 kg/m2) and Obese (n = 30, BMI ≥ 30 kg/m2).Results: The serum adenosine deaminase activity was significantly increased in overweight and obese subjects and as well as in combined overweight and obese group as compared to control (P < 0.0001).Conclusion: Adenosine, an endogenous anti-inflammatory metabolite, is increased in response to inflammation produced by adipose tissue in obesity. However, adenosine deaminase acts on adenosine to convert it into inosine, thereby increasing adenosine deaminase activity in overweight and obese Indian subjects.

Serum adenosine deaminase activity as a predictor of disease severity in ulcerative colitis

Background and aim Ulcerative colitis (UC) is a chronic inflammatory disease characterized by recurrent inflammation and ulcerations of colonic mucosa and an inappropriate and delayed healing. Adenosine deaminase (ADA) is a cytoplasmic enzyme involved in the catabolism of purine bases, capable of catalyzing the deamination of adenosine, forming inosine in the result process. Although ADA has been shown to increase in several inflammatory conditions, there are no literature data indicating an alteration in UC. Methods This study evaluated the activity of total ADA in serum of 43 patients with UC and 18 healthy controls. Patients’ age, disease duration, drug intake, and other medical history were all noted for each subject. Complete blood count, erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP) were determined for both patients and controls. Correlation analysis was also performed between ADA and other inflammation markers of UC. Results Serum mean ADA levels were 11.12 ± 2.03 and 7.99 ± 2.04 U/l for patients with UC in active state and in remission and 8.55 ± 2.26 U/l in the healthy control group. Mean serum ADA levels were significantly elevated in active UC patients compared with patients with UC in remission and control groups. Overall accuracy of ADA in determination of active UC was 83.7 with sensitivity 83.3%, specificity 84.2%. Conclusions Serum ADA levels were found to be elevated in UC patients in active state suggesting a partial role of activated T-cell response in the disease pathophysiology. Further randomized controlled studies are warranted to demonstrate the role of ADA in UC patients, with a special interest in specifically targeted therapies against ADA for achieving disease remission.

Serum myeloperoxidase and adenosine deaminase activities in polycystic ovary syndrome

Objective: To evaluate serum myeloperoxidase (MPO) and adenosine deaminase activities and to investigate whether there was a correlation between these and sex hormones, and lipids in polycystic ovary syndrome. Design and methods: Forty-five women with polycystic ovary syndrome and 40 healthy controls were included in this study. Leukocyte counts, serum MPO and adenosine deaminase activities, and high-sensitive C-reactive protein (hs-CRP), sex hormone and lipid levels were assessed. Results: Leukocyte counts and serum MPO activities were higher (p < 0.01 and p < 0.05, respectively) and there was a significant correlation between serum adenosine deaminase and hs-CRP level in women with polycystic ovary syndrome (r = 0.853, p < 0.01). Serum MPO and adenosine deaminase levels did not correlate with sex hormones or lipid profiles in these patients. Conclusion: Polycystic ovary syndrome may involve an inflammatory process by increasing serum MPO activity independent of sex hormones, body mass index and lipid profiles.

Activity of cholinesterases and adenosine deaminase in blood and serum of rats experimentally infected withTrypanosoma cruzi

This study aimed to evaluate the activity of cholinesterases and adenosine deaminase (ADA) in blood and serum of rats infected with Trypanosoma cruzi. Twelve adult rats were used in the experiment divided into two uniform groups. Rodents from group A (control group) were non-infected and animals from group B served as infected, receiving intraperitoneally 3·3×10(7) trypomastigotes/each. Blood collection was performed at days 60 and 120 post-infection (PI) in order to evaluate the hemogram, blood activity of acetylcholinesterase, and serum butyrylcholinesterase and ADA activities. Hematological parameters did not differ between groups. A significant increase (P<0·05) of acetylcholinesterase activity was observed in blood while butyrylcholinesterase had a significant reduction (P<0·01) in serum of infected rats at days 60 and 120 PI. ADA activity in serum showed an inhibition in infected animals when compared to non-infected at day 120 PI. Based on these results, it is possible to conclude that the activity of cholinesterases and ADA were changed in animals infected with T. cruzi. The possible causes of these alterations will be discussed in this paper.

Serum adenosine deaminase may predict disease activity in rheumatoid arthritis

To determine the relationship between serum adenosine deaminase (ADA) and disease activity, and to develop a new disease activity index based on serum ADA in rheumatoid arthritis (RA). Seventy RA patients were included. Disease activity based on Disease Activity Score 28-ESR (DAS28-ESR) and Disease Activity Score 28-CRP (DAS28-CRP) and serum ADA were measured. There were correlations when serum ADA compared with DAS28-ESR and DAS28-CRP. (R (2) = 0.014, 0.175, respectively, P values < 0.00). New disease activity index was developed by replacing ADA with ESR and CRP in DAS28-ESR and DAS28-CRP. There were strong correlations when new model compared with DAS28-ESR and DAS28-CRP. (R (2) = 0.94 and 0.95, respectively, P values < 0.00) The best new model values corresponding to DAS28-ESR values of 2.6, 3.2, and 5.1 were 2.79, 3.4, and 4.82, respectively; and new model values corresponding to DAS28-CRP values of 2.3, 2.7, and 4.1 were 2.1, 2.9, and 4, respectively. There were agreements when the new model compared with DAS28-ESR and DAS28-CRP for determination of patients in different disease activity categories. (Kappa = 0.81 and 0.71, respectively, P values < 0.00). The new disease activity index that applies serum ADA may help in predicting disease activity in RA.

Correlation of adenosinergic activity with superior efficacy of clozapine for treatment of chronic schizophrenia: a double blind randomised trial

It has been proposed that a deficit of adenosinergic activity could contribute to the pathophysiology of schizophrenia. The authors undertook this study to further evaluate the level of adenosine deaminase (ADA) in patients with chronic schizophrenia treated with monotherapy of haloperidol, risperidone or clozapine and correlation between the ADA level with response to treatment. The trial was a prospective, 8-week, double blind study of parallel groups of patients with chronic schizophrenia. Eligible participants in the study were 51 patients with chronic schizophrenia with ages ranging from 20 to 45 years. All participants were inpatients, in the active phase of illness, and met DSM-IV-TR criteria for schizophrenia. Patients were randomly allocated (17 patients in each group) to risperidone (6 mg/day) or haloperidol 15 mg/day or clozapine (300 mg/day). Serum ADA activity was measured at baseline and week 8. The plasma levels of ADA in patients with chronic schizophrenia who received clozapine were significantly higher than patients who received haloperidol. In addition, response to treatment was positively correlated with plasma levels of ADA only in the clozapine group (r = 0.46 and p = 0.04). The results indicate an increased activity of the enzyme ADA in the serum of schizophrenic patients being treated with clozapine and this increase may be correlated with clozapine's superior antipsychotic efficacy.

Serum adenosine deaminase activity in patients with systemic lupus erythematosus: a study based on ADA1 and ADA2 isoenzymes pattern

Adenosine deaminase (ADA) plays a crucial role in the development and maintenance of normal immune system. So, any immunological imbalances could associate with its altered activity in serum. This study evaluated the activity of total ADA and its isoenzymes in serum of 45 patients with systemic lupus erythematosus (SLE). Included were 23 patients with active SLE, 22 during the inactive phase of the disease, and 45 healthy subjects. Our results provided evidence that the significantly elevated total ADA activity in serum of SLE patients is correlated mainly with the increased ADA2 level. The highest mean ADA2 activity during the relapse phase of the disease could be an indication to the macrophages, the main source of ADA2. It might be concluded that ADA and its isoenzymes analysis in serum of patients could be used as a useful and non-invasive diagnostic tool in evaluation of SLE active phase and the disease severity.

Adenosine deaminase activity in serum, erythrocytes and lymphocytes of rats infected with Leptospira icterohaemorrhagiae

Leptospirosis is a systemic disease of humans and domestic animals, mainly dogs, cattle and swine. The course of human leptospirosis varies from mild to severe fatal forms and the most severe form of human leptospirosis is principally caused by Leptospira interrogans serovar icterohaemorrhagiae (L. icterohaemorrhagiae). The enzyme adenosine deaminase (ADA) plays an important role in the production and differentiation of blood cells. The aim of this study was to evaluate the activity of ADA in serum, erythrocytes and lymphocytes of rats infected with L. icterohaemorrhagiae, as compared with non-infected rats. Twenty-four adult rats, divided into two uniform groups (A and B) were used for the enzymatic assays. The animals in Group B were inoculated intraperitoneally with 2×108leptospires/rat, and the rodents in Group A (control) were not-inoculated. Blood collection was performed on days 5 and 15 post-infection (PI) and the blood used to assess the ADA activity. The infection by L.icterohaemorrhagiae altered erythrocyte count, hemoglobin concentration and hematocrit, causing a decrease in all these parameters on day 15 PI. Lymphocytes decreased significantly on day 15 PI, and ADA activity in serum was inhibited in infected rats on days 5 and 15 PI and its activity in erythrocytes were increased on day 5 PI. On day 5 PI, we found an increase in ADA activity in erythrocytes of infected rats. No correlation was observed between hematocrit and erythrocyte ADA activity on days 5 and 15 PI. The ADA activity was inhibited in rats infected on day 15 PI. A positive correlation (r2=60) was also observed between the number of lymphocytes and ADA activity in lymphocytes on day 15 PI (P<0.05). In conclusion, our results showed that the ADA activity is altered in serum, lymphocytes and erythrocytes in experimental infection by L.icterohaemorrhagiae in rats, concomitantly with hematological parameters.

Activity of the enzyme adenosine deaminase in serum, erythrocytes and lymphocytes of rats infected withTrypanosoma evansi

In Trypanosoma evansi infections changes in the haemogram are commonly observed, and the enzyme adenosine deaminase (ADA) plays an important role in the production and differentiation of blood cells. Thus, the aim of this study was to evaluate the activity of ADA in serum, erythrocytes and lymphocytes of rats infected with T. evansi compared to non-infected rats. Thirty adult rats were used, divided into 3 uniform groups. The animals in groups A and B were infected intraperitoneally with 2 x 10⁶ trypomastigotes/rat. Rodents from group C (control group), were not-infected. Blood collection was performed on days 4 and 20 post-infection (p.i.) in order to obtain acute and chronic infection stages of disease. The blood was used to assess the activity of ADA. In the blood, reduced haematocrit and increased lymphocytes were correlated with ADA activity in erythrocytes and lymphocytes. We observed reduction of ADA activity in serum and erythrocytes in rats infected with T. evansi compared to non-infected rats (P < 0.05). ADA activity in lymphocytes was decreased after 4 days, when the parasitaemia was high and increased after 20 days, when the number of circulating parasites was low. In conclusion, our results showed that the ADA activity was altered in serum, lymphocytes and erythrocytes of rats, concomitantly with haematological parameters, in experimental infection by T. evansi.

サルコイドーシス患者血清中のAdenosine deaminase (ADA) 活性値

サルコイドーシス患者血清中のAdenosine deaminase (ADA) 活性値

Serum adenosine deaminase activity and the total antioxidant capacity of plasma in pulmonary tuberculosis and non-tuberculosis pulmonary disease

The aim of this study was to investigate the level of serum adenosine deaminase (ADA) and the total antioxidant capacity (TAC) in pulmonary tuberculosis (PTB), non-tuberculosis pulmonary disease (non-PTB) and healthy subjects. Materials and methods: Serum ADA activity was measured using the Giusti and Galanti method, and the total antioxidant capacity of plasma was determined by the ferric reducing ability of plasma (FRAP) test. Results: The serum ADA levels were significantly higher (P < 0.001) in pulmonary TB (PTB, 19.78 ± 7.09 U/L), as well as in non-PTB patients (14.78 ± 4.65 U/L) when compared to healthy controls (10.02 ± 1.99 U/L). The sensitivity and specificity were found to be 71.7% and 63.3%, respectively, in distinguishing PTB from non-PTB. In distinguishing PTB from healthy subjects, the sensitivity and specificity were 87% and 93.3%, respectively. The TAC was significantly lower in PTB (485.2 ± 190.0 µM) and non-PTB patients (588.3 ± 195.8 µM), when compared to the controls (784.3 ± 190.0 µM; P < 0.001). Plasma antioxidative activity decreased in PTB and non-PTB patients when compared to the controls. Conclusion: We concluded that serum ADA activity is not a useful test to differentiate pulmonary TB from other respiratory diseases. The TAC is low in pulmonary TB, therefore supplementation with a suitable anti-oxidant may be useful.

Syzygium cumini inhibits adenosine deaminase activity and reduces glucose levels in hyperglycemic patients

Syzigium cumini (L.) Skeels from the Myrtaceae family is among the most common medicinal plants used to treat diabetes in Brazil. Leaves, fruits, and barks of S. cumini have been used for their hypoglycemic activity. Adenosine deaminase (ADA) is an important enzyme that plays a relevant role in purine and DNA metabolism, immune responses, and peptidase activity. ADA is suggested to be an important enzyme for modulating the bioactivity of insulin, but its clinical significance in diabetes mellitus (DM) has not yet been proven. In this study, we examined the effect of aqueous leaf extracts of S. cumini (L.) (ASC) on ADA activity of hyperglycemic subjects and the activity of total ADA, and its isoenzymes in serum and erythrocytes. The present study indicates that: (i) the ADA activity in hyperglycemic serum was higher than normoglycemic serum and ADA activity was higher when the blood glucose level was more elevated; (ii) ASC (60-1000 microg/mL) in vitro caused a concentration-dependent inhibition of total ADA activity and a decrease in the blood glucose level in serum; (iii) ADA1 and 2 were reduced both in erythrocytes and in hyperglycemic serum. These results suggest that the decrease of ADA activity provoked by ASC may contribute to control adenosine levels and the antioxidant defense system of red cells and could be related to the complex ADA/DPP-IV-CD26 and the properties of dipeptidyl peptidase IV (DPP-IV) inhibitors which serve as important regulators of blood glucose.

Serum adenosine deaminase activity in gestational diabetes mellitus and normal pregnancy

To our knowledge, there is no report regarding adenosine deaminase activity (ADA) in gestational diabetes mellitus (GDM). The aim of the present study was to investigate the level of serum ADA activity in normal pregnancy, GDM and normal non-pregnant women. The serum catalytic concentration of ADA was measured in samples of 20 GDM, 40 normal pregnant and 20 non-pregnant healthy women. Adenosine deaminase activity was significantly higher in GDM (24.30 +/- 8.04 IU/L) and pregnant women (23.88 +/- 8.66 IU/L) than those of normal non-pregnant individuals (11.85 +/- 3.23 IU/L) (P < 0.0001), but the ADA level was not significantly different from GDM and normal pregnant women (P = 0.97). Adenosine deaminase activity was significantly higher in GDM and pregnant individuals than normal group in this study. Previous reports in this field are contentious; therefore, ADA activity could be regulated in different population by immunologic and genetic factors.