Influence of Hyperthermic Environmental Temperature on Adenosine Deaminase Activity in Serum and Lymphoid Organs of Wistar Rats

Abstract

Aim: To analyze the influence of the hyperthermic environmental temperature (35 ± 1°C) on the adenosine deaminase activity (ADA) in tissues rich with lymphoid cells in Wistar rats. Material and Methods: The activity of ADA was examined in thymus, spleen, Payer’s patches, mesenteric lymph nodes and serum. The experimental animals (n = 11 male rats) were exposed on hyperthermic environment (35 ± 1°C) and relative air humidity of 20 - 30% within a period of 30 days. The control group of animals (n=10) were kept on room temperature (18 - 22°C). Results: The body weight was decreased, spleen mass, and the thymus mass were reduced. Activity of ADA in the thymus was significantly increased from 10.58 ± 1.57 U/g in the control group of rats to 13.38 ± 2.7 U/g in rats exposed on 35 ± 1°C (p<0.01), and in the spleen it raised from 5.17 ± 3.29 E/g to 7.8 ± 1.97 U/g (p<0.05). The ADA activity in the other lymphoid organs and serum showed no statistically significant changes between the two groups of examined rats. Conclusions: Influence of hyperthermic environment (35 ± 1°C, 30 days) provokes decrease in body weight, spleen and thymus relative mass, increase of the enzyme activity of ADA in thymus and spleen of rats, but there are no changes in the other lymphoid organs and tissues.