Poultry Feed Additives Research Articles

Oxidative damage under As3+ and/or Cu2+ stress leads to apoptosis and autophagy and may be cross-talking with mitochondrial disorders in bursa of Fabricius

Arsenic (As) exists in many forms in the whole natural environment, with As3+ the highest toxicity. Herein our study demonstrated that arsenic trioxide (As2O3) at a dose of 30 mg/kg caused serious oxidative damage to chickens' bursa of Fabricius (BF) in a time-dependent manner. Copper (Cu) is a necessary micronutrient and a key catalytic cofactor of many enzymes. We found excessive Cu (in the form of 300 mg/kg copper sulfate (CuSO4)) also induced severe oxidative stress (OxS), and its co-exposure with As3+ had a greater destructive power against oxidative system. Under electron microscope, swollen mitochondria, disappeared cristae and agglutinated chromatin were observed, accompanied by myeloid structure and autophagosome. The results showed apoptosis and autophagy occurred under the action of As3+ and Cu2+, and the situation was more serious in combined exposure group, which was further explained by terminal deoxynucleotidyl transferase (TdT)-mediated 2′-Deoxyuridine 5′-Triphosphate (dUTP) Nick-End Labeling (TUNEL). By quantitative real time polymerase chain reaction (RT-qPCR) and western blot, we found that mitochondrial dynamics were disordered under OxS, and the abnormal changes of B-cell lymphoma (Bcl)-2, p53, Bcl-2-interacting protein (Beclin)-1 and autophagy-related gene (ATG) 4B indicated the crosstalk between apoptosis and autophagy. In conclusion, apoptosis and autophagy of BF induced by As3+ and Cu2+ and mitochondrial disorder are closely related to the collapse of antioxidant system, and their connections are inseparable. Our results provide a reference for environmental risk prevention and selection of poultry feed additives and pesticides to avoid the health risks caused by As3+ and Cu2+ exposure.

Harnessing the antibacterial activity of Quercus infectoria and Phyllanthus emblica against antibiotic-resistant Salmonella Typhi and Salmonella Enteritidis of poultry origin.

Background and Aim:In a scenario of the ineffectiveness of the current drugs against antibiotic-resistant pathogens, the herbal extracts can serve as an alternative remedy. This study appraises the antibacterial potency of Quercus infectoria (gall), Phyllanthus emblica (fruit) individually and synergistically against antimicrobial-resistant (AMR) Salmonella Typhi and Salmonella Enteritidis in a time and dose-dependent manner. Further, the antibacterial phytocompounds were identified employing gas chromatography-mass spectrometry (GC-MS).Materials and Methods:Preliminary antibacterial activity of the plant extracts was assessed using the agar disk diffusion method. In vitro evaluations of Q. infectoria methanolic extract (QIME) and P. emblica methanolic extract (PEME) against S. Typhi and S. Enteritidis were carried out using plate count method.Results:QIME and PEME at a dose rate of 50 mg/ml and 25 mg/ml, respectively, had a complete bactericidal effect on AMR S. Typhi and S. Enteritidis whereas 10 log10 CFU/ml of exponential growth was seen in untreated control groups. At the lower concentrations, QIME and PEME had a significant bacteriostatic effect (3-6 log10 reduction of the test isolates). The synergistic antibacterial effect obtained from the combination of these two plant extracts at 12.5 mg/ml was superior (p<0.001) than the individual treatments. Phytochemical profiling indicated the presence of tannins, flavonoids, saponins, and terpenoids in both the plant extracts. GC-MS analysis of QIME and PEME revealed the presence of 16 and 15 antibacterial phytocompounds, respectively. Further 1, 2, 3 Benzenetriol was found as the prominent active principle.Conclusion:The findings validate that QIME and PEME are potential antibacterial agents against AMR S. Typhi, S. Enteritidis and can play a promising role in antimicrobial packaging, poultry feed additives and can also serve as a platform for formulating effective phytotherapeutics.

The quality of gosling meat depending on the level of lithium in mixed fodders

Micronutrients are an important component of a complete poultry feeding. Current detailed feeding standards provide guaranteed feed additives for farm poultry of the microelement complex. Among the normalized microelements there is no lithium, which according to the classification based on biological role for living organisms belongs to the group of conditionally essential elements. The purpose of the study was to investigate the effect of various doses of lithium in compound feed on the chemical composition and toxicological and biological parameters of caterpillar meat. The effect of additives of different doses of lithium (0.05 mg/kg, 0.10 and 0.15 mg/kg) in compound forages on the quality and safety of the products of slaughter of 70-day gosling was studied. The studies were conducted on gosling of the Legart breed. Feeding of gosling from day to 70 days of age was carried out by full-feed compound feeds. The birds of the experimental groups were additionally injected with lithium in the feed, mg/kg: the second group was 0.05; third – 0.10 and fourth – 0.15. The gosling of the control group did not receive lithium. Analysis of the results of the studies revealed differences between the control and experimental groups in the chemical composition of the muscular tissue of the gosling in favor of the latter. It was found that the introduction of lithium compound feed had a positive effect on the deposition of dry matter, protein and fat in the gosling of the experimental groups, which contributed to its increased energy and biological value. The best indicators of meat quality were in young animals, which during the growing period were fed compound feeds enriched with lithium at the rate of 0.1 and 0.15 mg/kg.

The Genus Allium as Poultry Feed Additive: A Review.

Simple SummaryThe routine and unregulated use of in-feed antibiotics as growth promoters in poultry have been linked to the development of antimicrobial resistance, a serious global threat to the human, animal, and environment health. Growing public health concerns about food and environmental safety intensified the search for effective antibiotic alternatives in poultry production. The aim of this review is to present the current state of knowledge on the use of alliums as effective poultry feed additives in relation to their effects on growth performance, disease infections, gut and immune modulation, and product quality.The genus Allium, belonging to the family Amaryllidaceae has been known since ancient times for their therapeutic potentials. As the number of multi-drug resistant infections has increased due to in-feed antibiotic usage in poultry, the relevance of alliums as feed additives has been critically assessed. Garlic and the other Allium species, such as onions, leek, shallot, scallion, and chives, have been characterized to contain a plethora of bioactive compounds such as organosulfur compounds, polyphenols, saponins, fructans, and fructo-oligosaccharides. Consequently, alliums have been validated to confer antioxidant, antibacterial, antiviral, immunostimulatory, gut homeostasis, and lipid- as well as cholesterol-lowering properties in poultry. This review intends to summarize recent progress on the use of edible alliums as poultry feed additives, their beneficial effects, and the underlying mechanisms of their involvement in poultry nutrition. Perspectives for future research and limitations are also briefly discussed.

English

Among the currently available poultry feed additives, natural herbs and plants have been widely advocated due to their reported widespread beneficial effects. Vernonia amygdalina is one of such potential feed supplements which have recently been reported as having a wide range of beneficial effects on production performance. This study was designed to evaluate the effect of varying levels of V. amygdalina leaf meal on growth performance, hematological parameters and as anticoccidial agent for broiler chicken. A total of one hundred and fifty day old marshal broiler chicks were randomly allotted to five dietary treatments with 30 birds per treatment, replicated thrice, in a completely randomized design. The treatments were: Treatment 1 (T1) served as control (positive control) with inclusion of coccidiostat but no inclusion of V. amygdalina, T2 served as negative control with no inclusion of either coccidiostat or V. amygdalina, T3 (200 g of V. amygdalina/ 150 kg of feed), T4 (400 g of V.amygdalina/ 150 kg of feed), and T5 (600 g of V. amygdalina/150 kg of feed). Significant differences (p &lt; 0.05) were observed in the growth performance characteristics.&nbsp; The result of hematological indices shows that there were no significant differences (p &gt; 0.05) across the treatment except for hemoglobin in T2 that has the lowest (8.04 g/dl) and T4 that has the highest (12.03 g/dl). Eosinophil&rsquo;s in T4 has the highest value (4.00%) and lowest in T5 (2.00%) and T2 (2.00%). However, all other parameters were within the normal range. It can be concluded that V. amygdalina can be used as anticoccidial in broiler chickens due to high fed conversion ratio and Eosinophil&rsquo;s observed in T3 (200 g) and T4 (400 g), respectively.&nbsp; Key words: Natural herbs, vernonia amygdalina, performance, haematological, coccidiostat. &nbsp

The complete mitochondrial genome of dark mealworm Tenebrio obscurus (Coleoptera: Tenebrionidae)

Mealworms are the most common edible insect and feed additives in poultry, livestock, and aquaculture production. In the present study, the complete mitochondrial genome of dark mealworm Tenebrio obscurus (Zunyi population) was sequenced and annotated. The 15,509 bp circular mitogenome contains a conserved set of 37 mitochondrial genes including 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and an A + T-rich region. The gene order is identical to the typical insect gene arrangement. Great differences were found in A + T-rich regions between Zunyi population and Guiyang population including large deletion and substitution. Phylogenetic analysis showed that T. obscurus clustered within Tenebrionoidea and was closely related to Tenebrio molitor.

Selection of Local Microorganism Solutions as Crude Fiber Digester in Areca catechu L. Peel Waste for Producing Poultry Feed Additives

Selection of Local Microorganism Solutions as Crude Fiber Digester in Areca catechu L. Peel Waste for Producing Poultry Feed Additives

Benefits of probiotics and/or prebiotics for antibiotic-reduced poultry.

Antibiotics have been used for many years as growth promoters. They contribute to build the immunocompetence (i.e. ability of the body to produce a normal immune response following exposure to an antigen) of birds against infectious diseases and as growth promoters. Antibiotics have been widely used as growth promoters in the field of animal production since 1940s. There is a hypothesis that is effect is brought about by dynamic biological interaction with the micro-flora in the intestine. In 1951, the United States Food and Drug Administration approved the use of antibiotics as animal additives to prevent disease in general and, in some cases, to improve efficiency without veterinary prescription. In the 1950s and 1960s, each European state approved its own national regulations about the use of antibiotics in animal feeds. However, using antibiotics may develop bacteria resistant to these drugs. Accordingly, the use of antibiotics has been minimized and replaced by effective dietary supplements such as probiotics and/or prebiotics that are claimed to enhance growth and positively modulate the immune response. The current review paper sheds light on the benefits of using probiotics and/or prebiotics in poultry feed versus the risk of using antibiotics and the mechanisms by which they exert their effects, as well as the economic analysis of using these beneficial additives in poultry feed.

Effect of natural growth promoters on immunity, and biochemical and haematological parameters of broiler chickens

Purpose : To investigate the efficacy of promising alternatives to antibiotic growth promoters (organic acids, phytobiotics, and their combinations) as feed additives in poultry feed. Methods : Different feed treatments were formulated with organic acids, phytobiotics and their combinations, and their effects on blood profile, serum enzymes and immunity parameters were evaluated in broilers at 21 and 42 days of age. Results : Cholesterol, triglyceride and HDL levels of the 21- and 42-day old broilers were significantly (p 0.05) in parameters except hematocrit, RBC, MCH, MCHC, WBC at age 21 days. However, among the serum enzymes assayed, only gamma-glutamyl transferase activity was altered for the modified feed group. Conclusion : These results suggest that supplementation with organic acids and phytobiotics can be used as alternatives to antibiotic growth promoters without interfering with the overall health and performance of broilers. Keywords : Broilers, Antibiotic growth promoters, Phytobiotics, Organic acids, Biochemical parameters

Coccidiostats in unmedicated feedingstuffs for poultry

Coccidiostats are compounds that are widely used as feed additives to prevent and treat coccidiosis, a contagious disease affecting mainly poultry, and which is associated with warm and humid conditions, as can be found on poultry farms. In Serbia and in the EU, specific coccidiostats are authorized as poultry feed additives. A wide range of these products is available for prevention (as additives) and treatment of coccidiosis (as veterinary medicinal products). The aim of this study is to present findings of residues of coccidiostats in unmedicated feed for chickens for fattening and laying hens as possible causes for coccidiostat residues in liver and eggs. The reasons for these compounds occurring in animal tissues and primary products of animal origin could be an inappropriate withdrawal period after the last administration of medicated feed or cross-contamination of unmedicated feed during the production on the same production line as medicated feedingstuffs, because of inadequate cleaning procedures and/or hygiene practices.

High-carotenoid biofortified maize is an alternative to color additives in poultry feed

Skin color in poultry is achieved by the addition of natural or synthetic pigments to feed. Crops used routinely in feed formulations offer an alternative cost-effective strategy to replace color additives if they are biofortified with sufficient levels of carotenoids. We tested the hypothesis that high-carotenoid (HC) maize, which was genetically engineered to accumulate high levels of β‐carotene, lutein and zeaxanthin in the endosperm, can replace carotenoid additives in poultry feed by performing two feeding trials using diets incorporating different maize lines with diverse carotenoid compositions: control (wild-type M37W, the parental line), HC, and standard yellow commercial maize supplemented with color additives (marigold flowers and red paprika extracts). The effects of dietary treatments on growth performance, health parameters, color evolution and carotenoid distribution were determined. We found that chickens fed on the HC diet grew normally and developed similar pigmentation to animals fed on a commercial diet supplemented with color additives, although yellowness was significantly higher in the commercial diet due to the high concentration of yellow xanthophylls. Lightness scores in chickens fed on the control, HC and commercial diets were 45.88±1.31, 44.32±1.10 and 44.29±0.99, respectively, in breast muscle, and 51.62±1.33, 49.66±0.96 and 50.10±1.16, respectively, in thigh muscle. Redness scores in chickens fed on the control, HC, and commercial diets were 0.36±0.26, 3.25±0.29 and 3.58±0.29, respectively, in breast muscle, and 1.28±0.37, 4.79±0.39 and 4.85±0.34, respectively, in thigh muscle. Yellowness scores in chickens fed on the control, HC, and commercial diets were 2.45±0.47, 7.61±0.64 and 9.66±0.73, respectively, in breast muscle, and 3.38±0.64, 10.00±1.10 and 12.64±0.97, respectively, in thigh muscle. High-carotenoid maize is therefore a cost-effective alternative to feed supplementation in the poultry industry.

Development and validation of a multi-analyte method for the regulatory control of carotenoids used as feed additives in fish and poultry feed

ABSTRACTCarotenoids are used in animal nutrition mainly as sensory additives that favourably affect the colour of fish, birds and food of animal origin. Various analytical methods exist for their quantification in compound feed, reflecting the different physico-chemical characteristics of the carotenoid and the corresponding feed additives. They may be natural products or specific formulations containing the target carotenoids produced by chemical synthesis. In this study a multi-analyte method was developed that can be applied to the determination of all 10 carotenoids currently authorised within the European Union for compound feedingstuffs. The method functions regardless of whether the carotenoids have been added to the compound feed via natural products or specific formulations. It is comprised of three steps: (1) digestion of the feed sample with an enzyme; (2) pressurised liquid extraction; and (3) quantification of the analytes by reversed-phase HPLC coupled to a photodiode array detector in the visible range. The method was single-laboratory validated for poultry and fish feed covering a mass fraction range of the target analyte from 2.5 to 300 mg kg–1. The following method performance characteristics were obtained: the recovery rate varied from 82% to 129% and precision expressed as the relative standard deviation of intermediate precision varied from 1.6% to 15%. Based on the acceptable performance obtained in the validation study, the multi-analyte method is considered fit for the intended purpose.

The Effect of Adding Hesperidin, Diosmin, Quercetin and Resveratrol Extracts to Feed for Turkey Hens on Selected Immunological and Biochemical Blood Indices

Abstract It was postulated that naturally occurring phenolic compounds obtained from various plant species may have potential use as feed additives for poultry. Therefore the aim of the study was to compare extracts of hesperidin, diosmin, quercetin and resveratrol in terms of their health-promoting (particularly immunostimulatory) effect on turkeys at different ages. The experiment was conducted on 720 Big 6 turkey hens assigned to 6 experimental groups of 120 individuals (6 repetitions with 20 birds each). The turkey hens in group G-C were the control, receiving a basal compound feed with no experimental additives. The turkey hens in the remaining groups, from the first to the 16th week of life, received a basal diet containing hesperidin (group G-H), diosmin (group G-D), quercetin (group G-Q) or resveratrol (group G-R) in the amount of 200 g per tonne of feed. Ht, Hb, RBC, WBC, lysozyme activity, %PC, IgA, IL-6, GLU, TP and minerals were determined in blood samples. The addition of quercetin or resveratrol in the amount of 200 g per tonne of feed was found to have a beneficial effect on haemoglobin synthesis and phosphorus availability, and may also modulate immunity in turkey hens.

Effect of Thyme Essential Oil Supplementation on Thymol Content in Blood Plasma, Liver, Kidney and Muscle in Broiler Chickens

The absorption and metabolism of phytogenic feed additives in poultry is studied related to the metabolism and deposition of their main compounds in tissues intended for food production. Fifty-six non-sexed Ross 308 broilers were allocated to seven dietary treatments and fed a diet containing graded levels of thyme (Thymus vulgaris L.) essential oil (EO) (0, 0.01, 0.02, 0.03, 0.04, 0.05, 0.1%, w/w). Thymol concentration was measured in plasma, liver, kidney and breast muscle tissue using solid phase micro-extraction followed by gas chromatography/mass spectrometry. We found the highest concentrations of thymol in kidney and plasma, and the lowest in breast muscle and liver. Thymol content in plasma and kidney significantly increased when 0.05 and 0.1%, w/w, EO and in liver and breast muscle only when 0.1%, w/w, EO was added to the diet (p<0.05). Our results indicate intensive metabolism of thymol in liver and its accumulation in kidney tissue. We confirm low deposition of thymol in the muscle tissue. It is necessary to.-keep in mind the selection of a sufficient concentration of EO in the feed additive for animals without the risk of thymol residues in edible tissues.

Bacillus species are Superior Probiotic Feed-Additives for Poultry

Bacillus species are Superior Probiotic Feed-Additives for Poultry

Nutrient assessment of olive leaf residues processed by solid-state fermentation as an innovative feedstuff additive.

Olive leaf residue feedstuff additives were prepared by solid-state fermentation (SSF), and its feeding effects on broiler chickens were examined. The fermentation's nutrient value, that is, protein enrichment, cellulase activity, tannic acid degradation and amino acid enhancement, was determined. The effect of different strains, including molds (Aspergillus niger, Aspergillus oryzae and Trichoderma viride) and yeasts (Candida utilis, Candida tropicalis and Geotrichum candidum), and the fermentation time on the nutrient values of the feedstuff additives was investigated. The experimental results showed that the optimal parameters for best performance were A.niger and C.utilis in a 1:1 ratio (v/v) in co-culture fermentation for 5days. Under these conditions, the total content of amino acids in the fermented olive leaf residues increased by 22·0% in comparison with that in the raw leaf residues. Both Glutamic acid and Aspartic acid contents were increased by more than 25·4%. Broiler chickens fed with different amounts of feedstuff additives were assessed. The results demonstrated that the chicken weight gains increased by 120%, and normal serum biochemical parameters were improved significantly after 10% of the feedstuff additives were supplemented to the daily chicken feed for 28days. The co-culture combination of A.niger and C.utilis with SSF for olive leaf residue had the best nutrient values. The addition of 10% fermented olive leaf residue facilitated the chicken growth and development. This study reveals that olive leaf residues fermented by SSF exhibited considerable potential as feed additives for feeding poultry.

Sumac (Rhus Coriaria L.) and Ginger (Zingiber Officinale) as Feed Additive in Poultry Nutrition

ABSTRACT: In the last decade, there has been growing interest in the use of natural herbs and medicinal plants as feed additives in poultry diets to maximize their potential output. The European Union banned the use of antibiotic feed additives and the scientists are trying to find alternatives, one of the best options is herbs. Researchers particularly look for medicinal herbs that can affect such parameters as growth or treatment of certain diseases. .Medicinal plants have been used in poultry diets as feed additives to increase poultry performance, health and the quality of their products and their extracts have been tested in the diets of poultry as potential alternatives to antibiotics on growth promotion. Also, poultry has seen the greatest increase in production and gain, this trend will likely continue. Both poultry meat and eggs are well positioned to meet demands for increased supply from our growing world population. The interest to use the medical plants is mainly due to safety and healthy for human and less cost of herbs compared with synthetic chemical drugs and some of them decrease the level of serum lipids and lead to improve immune function in humans and also for animals. Therefore, The aim of this review is to evaluate information on the use of medical herbs including Sumac ( Zingiber officinale ) and Ginger ( Rhus coriaria L .) as feed additives, with the emphasis on to improve poultry performance (laying hens and broiler). However, a systematic approach toward the efficacy and safety of phytogenic compounds used as feed additives for poultry is still lacking. Key Words: feed additive, ginger, poultry nutrition, sumac

The application impact of an origanum, anise and citrus oils blend in horses nutrition

DOI: 10.15414/afz.2015.18.04.103–105 Received 1. July 2015 ǀ Accepted 28. August 2015 ǀ Available online 7. December 2015 The aim of the paper was to analyse the origanum, anise and citrus oils blend as a potential phytogenic feed additive in horse’s nutrition. Several studies in the World analyze the effect of plant origin aromatic additives on blood serum indicators and nutrients digestibility in horses. Origanum and anise contains many chemical compounds as carvacrol, thymol and anethol, which can be beneficially in metabolism and nutrients digestion. Some studies shows, that these additives have insignificant (P>0.05) effect on blood serum indicators, but there are some studies, which reported positive significant (P<0.05) effect on serum cholesterol and triglycerides contents. The main positive effect of origanum and anise is in nutrients digestibility improving. These herbs can improve the digestibility of dry matter, organic matter, nitrogen free extract, crude fibre, ADV and NDV (P<0.05), as well as Mg and K from feed rations (P<0.05). Some antagonistic results published in this area can be affecting in dosage of active compounds, which have often antinutritive effects. Keywords : equine, additives, aromatic plants, feed rations References CAPCAROVA, M. and KOLESAROVA, A. (2010) Beneficial substances affecting internal milieu of animals. Nitra: Slovak University of Agriculture. EPP, T.S. et al. (2005) The effect of herbal supplementation on the severity of exercise-induced pulmonary haemorrhage. In J. Equine and Comp. Exerc. Physiol., vol. 2, pp. 17-25. FORS, M. 2009. Herbs for horses. Uppsala: Swedish University of Agricultural Science. GALIK, B. et al. (2011) The effect of phytoadditives on macroelements digestibility of sport horses. In J. Centr. Europ. Agricul., vol. 12, pp. 390-397. DOI:http://dx.doi.org/10.5513/JCEA01/14.3.1308 GALIK, B. et al. (2012a) The effect of phytogenic additive on in vivo saccharides digestibility of sport horses. In Pharm. Comm., vol. 2, pp. 3-6. DOI: http://dx.doi.org/10.5530/pc.2013.1.2 GALIK, B. et al. (2012b) The effect of different macromineral intakes on mineral metabolism of sport horses. In Acta Vet. Brno, vol. 81, pp. 113-117. DOI: http://dx.doi.org/10.2754/avb201281020113 GALIK, B. (2012c) The effect of fytogenic feed addtives on nutrients utilization in non-ruminants: Assoc. Prof. thesis. Nitra: Slovak University of Agriculture (in Slovak) GALIK, B. et al. (2013) The effect of phytoadditives on biochemical indicators and nutrients digestibility in sport horses. In J. Centr. Europ. Agricul., vol. 14, pp. 219-227. DOI: http://dx.doi.org/10.5513/JCEA01/14.3.1308 KAMEL, C. (2000) Natural plant extracts: Classical remedies bring modern animal production solutions. In Proceedings of the 3rd Conference on sow feed manufacturing in the Mediterranean region, Reus, March 22-24, 2000. Reus: Spain, pp. 31-38. MORGAN, L.M. et al. (2007) Effect of yeast culture supplementation on digestibility of varying forage quality in mature horses. In J. Equine Vet. Sci., vol. 27, pp. 260-265. PEARSON, W. et al. (2007) Pilot study investigating the ability an herbal composite to alleviate clinical sings of respiratory dysfunction in horses with recurrent airway obstruction. In Can. J. Vet. Res., vol. 71, pp. 145-151. STRAKOVA, E. et al. (2007) Positive effect of plant-based diet on the performance and health of laying hens. In Acta Vet. Brno, vol. 76,pp. 31-37. DOI: http://dx.doi.org/10.2754/avb200776S8S031 STEINER, T. (2006) Managing gut health: Natural growth promoters as a key to animal performance. Nottingham: Nottingham University Press. Steiner, T. (2009) Phytogenics in animal nutrition: Natural concepts to optimize gut health and performance. Nottingham: Nottingham University Press. SUCHÝ, P. et al. (2002) Replacement of animal origin feed by plant origin feed in the diet of broiler chickens. In Czech J. Anim. Sci., vol. 47, pp. 365-373. TURNER, K.K. et al. (2006) Bee pollen product supplementation to horses in training seems to improve feed intake: a pilot study. In J Anim. Physiol. Nutr., vol. 90, pp. 414-420. DOI: http://dx.doi.org/10.1111/j.1439-0396.2006.00621.x WINDISH, W. et al. (2008) Use of phytogenic products as feed additives for swine and poultry. In J. Anim. Sci., vol. 86, pp. 140-148. DOI: http://dx.doi.org/10.2527/jas.2007-0459. ZENG, Z. et al. (2015) Essential oil and aromatic plants as feed additives in non-ruminant nutrition: a review. In J. Anim. Sci. Biotech, vol. 6, pp. 1-10. DOI: http://dx.doi.org/10.1186/s40104-015-0004-5

Alternative feed additives in poultry

A possible bacterial pathogens resistant dissemination due to presence of medication residues in animal origin products and consumer market demand for quality products led to restriction on antibiotics administered in chicken feed, and the search for alternatives such as probiotics, prebiotics, symbiotic and phytogenic additives by offering favorable conditions to beneficial microorganisms to gastrointestinal tract development, immune stress reduction. Therefore, the objective of this study was to report alternative additives effect (probiotics, prebiotics, and synbiotics phytogenic additives) in poutry diet

Comparison of Microbiological and Probiotic Characteristics of Lactobacilli Isolates from Dairy Food Products and Animal Rumen Contents.

Lactobacilli are employed in probiotic food preparations and as feed additives in poultry and livestock, due to health benefits associated with their consumption. The objective of this study was to evaluate and compare the probiotic potential of ten lactobacilli strains isolated from commercial dairy food products and animal rumen contents in New Zealand. Genetic identification of the isolates revealed that all belonged to the genus Lactobacillus, specifically the species L. reuteri, L. rhamnosus and L. plantarum. All isolates did not show any haemolytic behaviour. Isolates of dairy origin showed better tolerance to low pH stress. On the other hand, rumen isolates exhibited a higher tolerance to presence of bile salts. All isolates exhibited resistance to aminoglycoside antibiotics, however most were sensitive to ampicillin. Isolates of rumen origin demonstrated a higher inhibitory effect on Listeria monocytogenes, Enterobacter aerogenes and Salmonella menston. Bacterial adherence of all isolates increased with a decrease in pH. This screening study on lactobacilli isolates has assessed and identified potential probiotic candidates for further evaluation.