Additional Coverage Research Articles

Increased Rate of Early Periprosthetic Joint Infection in Total Hip Arthroplasty With the Use of Alternatives to Cefazolin Despite Additional Gram-Negative Coverage.

BackgroundPeriprosthetic joint infection (PJI) remains one of the most devastating complications following total joint arthroplasty. Appropriate prophylactic antimicrobial administration and antibiotic stewardship are major factors impacting the risk of PJI in total hip arthroplasty (THA). The purpose of our study was to evaluate whether cefazolin administration was superior to noncefazolin antibiotics in prevention of PJI after primary THA.Material and methodsA review of 9910 patients undergoing primary THA from 2013 to 2019 at a single institution was conducted. The primary outcome was PJI within 90 days of surgery. The Musculoskeletal Infection Society definition of PJI was used for this analysis. Groups were those receiving cefazolin + expanded gram-negative antimicrobial prophylaxis (EGNAP) and those receiving an alternative to cefazolin + EGNAP. Chi-square tests were conducted to determine statistical significance. Multivariate logistic regression was performed to eliminate confounders.Results9028 patients received cefazolin + EGNAP, and 882 patients received an alternative to cefazolin + EGNAP. PJI rate using the Musculoskeletal Infection Society criteria was 0.82% (81/9910). PJI rate in the cefazolin + EGNAP group was 0.75% (68/9028). In the group receiving an alternative to cefazolin + EGNAP, the PJI rate was 1.47% (13/882). This difference was statistically significant (P = .023). On multivariate analysis, the odds ratio for developing PJI when an alternative to cefazolin was used was 2.05 (P = .022). When comparing alternatives, there remained a statistically significant increased PJI rate when the alternative used was clindamycin (odds ratio 2.65, P = .007).ConclusionOur data demonstrate that in the presence of EGNAP in THA, there was a higher PJI rate when clindamycin was given as an alternative to cefazolin. The number of THA patients receiving alternatives to cefazolin must be minimized.Level of EvidenceIII, Retrospective Cohort Study.

Study on film cooling for aero-engine turbine endwalls with various small-scale surface structures

In this study, a novel endwall modification by adding small-scale ribs onto a turbine endwall surface is proposed to reduce thermal loads on the endwall. The small-scale ribs are positioned in a streamline-curved and an axially-straight array patterns and, square and triangular shapes are imposed to the cross section of the ribs. The feasibility of improving purge air cooling by the ribs is investigated by using well-validated computational fluid dynamics (CFD) methods. Comprehensive comparisons of cooling performance among the ribbed endwall cases are made by examining cooling effectiveness, heat transfer, and aerodynamic losses. Detailed flow topologies are presented to reveal flow mechanisms involved in the actions of the ribs within the turbine passage flow. Particularly, secondary-order effects of the ribs on the turbine airfoil surfaces are also documented, which is expected to provide three-dimensional boundary conditions for the turbine aerodynamic and thermal analyses. Comparisons between the ribbed endwall cases and a smooth endwall case show that the endwall modification by adding small-scale ribs is effective in reducing thermal loads on the endwall via enhancing cooling effectiveness and, in some cases, reducing heat transfer levels. The thermal load reduction can be further improved by increasing purge air flow ratio. However, as additional vorticities are introduced by the ribs, slight aerodynamic penalty is generated. Among the four rib cases, the straight triangular-shaped rib case has the lowest overall cooling performance for the endwall. Additionally, secondary-order cooling effects are visible on the airfoil suction surface for all rib cases but only the straight rib cases can generate additional purge air coverage on the airfoil pressure surface, without significantly changing pressures in that region. This work offers a new option of thermal management techniques for the turbine endwall and the results provide guidelines regarding endwall modifications of such sort.

The benefit of tissue sealant on urethroplasty in hypospadias patients – A systematic review and meta-analysis

Background: Hypospadias was ranked second after undescended testis as the most prevalent congenital abnormality in newborn males. Hypospadias can be successfully repaired through multiple surgeries in the majority of children. Postoperative complications were not rarely seen after surgeries, such as urethrocutaneous fistula (UCF), meatal stenosis, and glans breakdown. Tissue sealant application in hypospadias repair serves as additional suture line coverage and reduces the post surgery complications5.However, the effects of sealants usage during urethroplasty are still uncertain. This review aimed to know the effects of tissue sealant usage on patients with hypospadias who undergo urethroplasty. Methods: The study was reported based on the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. Literature was searched on PubMed, Embase, and Scopus following PRISMA guidelines. The search was conducted on October 12th, 2021, using the search term ("glue" OR "sealants" OR "tissue glue" OR "tissue sealant" OR "tissue adhesive") AND ("hypospadias" OR "urethrocutaneous fistula" OR "urethral repair" OR "urethroplasty" OR "hypospadiology"). Result: Systematic searching from all databases resulted in 160 potential articles. After a full-text review, eight articles were included in this study. UCF complication was reported in all studies. The occurrence of complication reported by all studies was urethrocutaneous fistula. Several studies also reported tissue edema and flap-related complications. Tissue sealant had no significant effect in reducing meatal stenosis. Conclusions: This systematic review revealed additional benefits from several types of tissue sealant in hypospadias repair surgery. Fibrin sealant application over the urethroplasty suture line in hypospadias repair offers a water-proof coverage and may enhance the outcome from the surgery.

The SAMBA tool uses long reads to improve the contiguity of genome assemblies.

Third-generation sequencing technologies can generate very long reads with relatively high error rates. The lengths of the reads, which sometimes exceed one million bases, make them invaluable for resolving complex repeats that cannot be assembled using shorter reads. Many high-quality genome assemblies have already been produced, curated, and annotated using the previous generation of sequencing data, and full re-assembly of these genomes with long reads is not always practical or cost-effective. One strategy to upgrade existing assemblies is to generate additional coverage using long-read data, and add that to the previously assembled contigs. SAMBA is a tool that is designed to scaffold and gap-fill existing genome assemblies with additional long-read data, resulting in substantially greater contiguity. SAMBA is the only tool of its kind that also computes and fills in the sequence for all spanned gaps in the scaffolds, yielding much longer contigs. Here we compare SAMBA to several similar tools capable of re-scaffolding assemblies using long-read data, and we show that SAMBA yields better contiguity and introduces fewer errors than competing methods. SAMBA is open-source software that is distributed at https://github.com/alekseyzimin/masurca.

The structural coverage of the human proteome before and after AlphaFold.

The protein structure field is experiencing a revolution. From the increased throughput of techniques to determine experimental structures, to developments such as cryo-EM that allow us to find the structures of large protein complexes or, more recently, the development of artificial intelligence tools, such as AlphaFold, that can predict with high accuracy the folding of proteins for which the availability of homology templates is limited. Here we quantify the effect of the recently released AlphaFold database of protein structural models in our knowledge on human proteins. Our results indicate that our current baseline for structural coverage of 48%, considering experimentally-derived or template-based homology models, elevates up to 76% when including AlphaFold predictions. At the same time the fraction of dark proteome is reduced from 26% to just 10% when AlphaFold models are considered. Furthermore, although the coverage of disease-associated genes and mutations was near complete before AlphaFold release (69% of Clinvar pathogenic mutations and 88% of oncogenic mutations), AlphaFold models still provide an additional coverage of 3% to 13% of these critically important sets of biomedical genes and mutations. Finally, we show how the contribution of AlphaFold models to the structural coverage of non-human organisms, including important pathogenic bacteria, is significantly larger than that of the human proteome. Overall, our results show that the sequence-structure gap of human proteins has almost disappeared, an outstanding success of direct consequences for the knowledge on the human genome and the derived medical applications.

Experimental and numerical studies on the thermal and electrical performance of a CdTe ventilated window integrated with vacuum glazing

The application of PV double skin ventilated windows (PV-DSV) can reduce intense sunlight from entering room and meantime lower the cell temperature. Vacuum glazing is superior in thermal insulation. To further strengthen the thermal insulation of the PV-DSV, this paper proposes an integrated vacuum glazing with PV double-skin ventilated window (VPV-DSV), which employs the CdTe PV glazing and the vacuum glazing as the front and back glazings. Thermal and electrical performance of the VPV-DSV was investigated and compared with the PV vacuum insulating glazing unit (VPV-IGU) through experiment and simulation. Comparison of PV-DSV with different types of back glazing was made to explore the influence of back glazing types in PV-DSV. Furthermore, influence of the window design factors was explored. Results indicated that the use of vacuum glazing improved the thermal insulation of PV-DSV and resulted in 59.8% reduction of the U-value. VPV-DSV reduced more cooling load, but VPV-IGU outperformed VPV-DSV in thermal insulation. Owing to a small temperature coefficient of the CdTe cell, their power output was similar. In addition, the WWR and PV coverage had more obvious impacts on the performance of VPV-DSV than the emissivity of low-e coating.

Effectiveness of introduction of JEV vaccination into routine immunization program in a tribal district of Odisha.

ABSTRACTBackground:A severe outbreak of Japanese encephalitis (JE) and acute encephalitis syndrome (AES) with high case fatality among tribal children was reported from Malkangiri district of Odisha, during September to November 2016 affecting 336 children with 103 deaths. Following the outbreak, a mass vaccination campaign was introduced in Malkangiri district in2017. In 2018, the JE vaccine was introduced into the routine immunization program as per National Immunization Schedule. Our study surveys the JE vaccination coverage among children of Malkangiri and the incidence of JE cases for a period of three years.Methodology:The current study was conducted by establishing prospective and retrospective AES surveillance system and household vaccine coverage surveys in Malkangiri district. In the target population, the vaccination coverage survey was undertaken and also additional immunization coverage data from sub-centers was collected.Results:After 2016 JE outbreak, a mass vaccination campaign was carried out in children up to 15 years of age, where 96% of children were covered in the district in 2017 and only four AES cases were detected. Under routine immunization program, the vaccine coverage for the year 2018 was 68% for JE-1 dose and 37% for JE-2 dose. There were 8 AES cases detected in 2018 out of which four children died. Vaccination coverage for 2019 was 97% for JE-1 dose and 84% for JE-2 dose. The AES cases detected in 2019 was nil.Conclusion:Vaccination against JEV and AES surveillance systems has an important role in prevention and control of AES outbreaks.

Maximizing access in transit network design

This study adopts an Access-Oriented Design (AOD) framework for optimizing transit network design. We present and demonstrate a method to evaluate the best combination of local and express alternative transit system designs through the novel concept of ‘iso-access lines’. Two bus network system designs were explored for a greenfield development in suburban Sydney: through-routed transit lines (T-ways) with higher speeds and more direct service, but longer access and egress times, and local routes that provide additional spatial coverage. We developed scenarios with T-ways only, local routes only, and both, and computed transit access to jobs as a cumulative-opportunities measure for each scenario. Local routes offer greater overall access, while T-ways provide greater access-per-unit-cost. The optimal combination of the two was established by generating ‘iso-access’ lines and determining access-maximizing combinations for a given cost by applying production-theory principles. For 15-min access, the optimal combinations had T-way service frequency equivalent to 0.48 times that of local routes. This ratio increased to 1.45, 2.05 and 2.63 for 30-min, 45- min and 60-min access respectively. In practice, the method can be applied to determine optimal transit combinations for any given budget and desired access level.

Fate of the Dissected Thoraco-Abdominal Aorta Distal to TEVAR (Thoracic Endovascular Aortic Repair) for Complicated Acute and Subacute Type B Aortic Dissection

Purpose: This study assessed morphological changes in the aortic true and false lumens during follow-up of patients undergoing TEVAR (Thoracic Endovascular Aortic Repair) for complicated acute and subacute type B dissection. The study analyzes the effectiveness of TEVAR in preventing distal aneurysmal progression. Materials and Methods: All patients between 2009 and 2019 undergoing TEVAR for complicated acute and subacute type B dissection at the study institution were retrospectively reviewed. Maximal diameters were measured on the proximal descending aorta right below the left subclavian artery, thoraco-abdominal junction right above the celiac trunk, and infrarenal aortic right above the inferior mesenteric artery, pre-operatively and during follow-up, analyzing either expansion or shrinkage of true and false lumens at these 3 sites. Results: Forty-one patients were included. Thirty-day incidence of death, stroke, paraplegia, and visceral ischemia was, respectively, 8% (n = 4), 6% (n = 3), 2% (n = 1), and 2% (n = 1). Three patients (6%) died from intervention-related cause. Mortality was 17% (n = 8) during a mean follow-up of 54 months. One patient had aneurysmal dilation of the descending aorta needing additional coverage and only 2 (4%) developed thoraco-abdominal aneurysms requiring re-intervention. In the remaining patients, both significant expansion of the true lumen and shrinkage of false lumen were observed at all 3 sites. Conclusion: Proximal coverage of the main entry tear appears to prevent aneurysmal progression in most patients (96%). With such promising results, TEVAR should be considered as a first-line treatment in acute and subacute type B dissection.

Satellite Observation of the Marine Light-Fishing and Its Dynamics in the South China Sea

The South China Sea (SCS) is one of the most important fishery resource bases in the world. Marine fisheries, as a crucial component of regional food security and national revenue, raise wide concern about marine ecology, social-economic and political consequences at regional, national and local scales. The large-scale dynamic detection and analysis of fishing activity in the SCS is still unclear because of the accessibility of in-site data, finite automatic identification system (AIS) usage, complex geopolitics and poor additional data coverage. Nighttime light imagery (NTL) derived from low light imaging sensors and the popularity of light fishing in the SCS offers a unique way to unveil fishing activities and its dynamics. In this study, we proposed a set of algorithms for automatic detection of nighttime fishing activity and provided the first large-scale dynamic analysis of nighttime fishing activity in the SCS using monthly Visible Infrared Imaging Radiometer Suite (VIIRS) images between 2012 and 2019. The proposed method effectively minimized the spatio-temporal fluctuations in radiance values of background and their implications to ship detection by integrating high radiance gradient detection and local adaptive thresholding. Further, nighttime fishing activity trajectories were decomposed into trend and seasonal components by using Hilbert-Huang transformation (HHT) to accurately access general trends and the seasonality of nighttime fishing activity in the SCS. The typical subregions analysis, environmental driver analysis, correlation coefficient analysis and hot spot analysis were integrated to characterize the nighttime fishing activity. It appears that the nighttime fishing activity in the SCS exhibited spatio-temporal variability and heterogeneity and was shaped by policy and natural factors such as holidays, annual Chinese fishery moratoria in the Chinese Exclusive Economic Zone (EEZ) and seasonal tropical storm activity.

Preparation and Characterization of Fenofibrate Microparticles with Surface-Active Additives: Application of a Supercritical Fluid-Assisted Spray-Drying Process.

In this study, supercritical fluid-assisted spray-drying (SA-SD) was applied to achieve the micronization of fenofibrate particles possessing surface-active additives, such as d-α-tocopheryl polyethylene glycol 1000 succinate (TPGS), sucrose mono palmitate (Sucroester 15), and polyoxyethylene 52 stearate (Myrj 52), to improve the pharmacokinetic and pharmacodynamic properties of fenofibrate. For comparison, the same formulation was prepared using a spray-drying (SD) process, and then both methods were compared. The SA-SD process resulted in a significantly smaller mean particle size (approximately 2 μm) compared to that of unprocessed fenofibrate (approximately 20 μm) and SD-processed particles (approximately 40 μm). There was no significant difference in the effect on the particle size reduction among the selected surface-active additives. The microcomposite particles prepared with surface-active additives using SA-SD exhibited remarkable enhancement in their dissolution rate due to the synergistic effect of comparably moderate wettability improvement and significant particle size reduction. In contrast, the SD samples with the surface-active additives exhibited a decrease in dissolution rate compared to that of the unprocessed fenofibrate due to the absence of particle size reduction, although wettability was greatly improved. The results of zeta potential and XPS analyses indicated that the surface-active additive coverage on the surface layer of the SD-processed particles with a better wettability was higher than that of the SA-SD-processed composite particles. Additionally, after rapid depletion of hydrophilic additives that were excessively distributed on the surfaces of SD-processed particles, the creation of a surface layer rich in poorly water-soluble fenofibrate resulted in a decrease in the dissolution rate. In contrast, the surface-active molecules were dispersed homogeneously throughout the particle matrix in the SA-SD-processed microparticles. Furthermore, improved pharmacokinetic and pharmacodynamic characteristics were observed for the SA-SD-processed fenofibrate microparticles compared to those for the SD-processed fenofibrate particles. Therefore, the SA-SD process incorporating surface-active additives can efficiently micronize poorly water-soluble drugs and optimize their physicochemical and biopharmaceutical characteristics.

WI-MAX NETWORK SIMULATION FOR SALAHADDIN UNIVERSITY NEW CAMPUS

Wireless Networks became very important in this latest decade and especially Wi-Max system because of high bit rate, handover flexibility and longer distance of mobility. Signal strength and coverage is one of the challenges for this systems which leads to affect directly on Quality of Service (QoS), this paper is an attempt to design and study of Wi-Max network for a certain coverage area, testing the coverage of the signal for the area that can hold the sufficient amount of data with less latency has been studied depending upon the Wi-Max advanced specification and data stream behavior benefit’s, some Wi-Max parameters like coverage, quality of service, data rate are optimized, the study and design has been done using OPNET modeler used in the a simulation of the certain coverage area, in this paper the new campus of university of Salahaddin has been used on supposed area of about 4 X4 km. The results show a perfect bit rate as a bit stream for the global characteristics in which is sufficient to the students and teachers of the university in addition of providing the mobility by high performance. It is needed to design a wireless computer network to Salahaddin University to improve mobility and to let the teachers and students to stay connected at any time in any university location Especially with the nowadays learning techniques and requirements which needs both teacher and students to be online with each other especially like E-learning and online remount learning especially with the COVID-19 university campus blocking. Therefore, it is needed to cover overall area of the university campus and with additional coverage that exceed the university boundaries to improve the reliability of the hall network and to insure the required coverage area. To do that is very efficient to design an advanced computer network like Wi-Max with the most powerful and advanced hardware capabilities to full-fit the teachers and students requirement of fast net browsing and download in addition in e-learning bases it is very regular to use video conferencing or video streaming which normally needs a very fast network data transfer

1080 An Audit on Compliance with Recommended Duration of Surgical Antimicrobial Prophylaxis in St. Vincent’s University Hospital, Dublin, Ireland

Abstract Aim Surgical site infection (SSI) is a common healthcare-associated infection, and a frequent cause of post-operative morbidity. SSI comprises any infection of the operative incision, cavity or involved organ, that occurs within the 30-day post-operative period. Antimicrobial prophylaxis is critical in reducing SSIs. Our aim was to assess adherence to the recommended surgical antimicrobial prophylaxis guidelines in St. Vincent’s University Hospital (SVUH), as part of the hospital antimicrobial stewardship programme. Compliance of surgical services was measured against the recommended regimens described in the SVUH Antimicrobial Guidelines. Method Data on duration and choice of prophylaxis were prospectively gathered on inpatients undergoing elective surgery during a three-week period between August-September 2020. Patients undergoing transplant procedures, inpatients in the intensive care unit, and patients who moved off-site postoperatively were excluded. Results Eighty-one patients were included. Sixty-four (79%) were compliant with prophylaxis duration, while seventeen (21%) were not. Reasons for extended prophylaxis included three (18%) cases where there were intra-operative complications and five (29%) cases of routine prescribing of an additional 24-hour coverage. Nine (53%) did not document the reason for extended cover. Conclusions Compliance with recommended duration of prophylaxis improved in comparison to prior audits. Among patients receiving extended prophylaxis, documentation of the indication was poor. Patients with evidence of post-operative infection may be better served with targeted antimicrobial therapy rather than extended prophylaxis. One reason for extended prophylaxis may be due to difficulty accessing guidelines on recommended prophylaxis duration, hence a targeted smartphone application was introduced to improve accessibility.

42 An Improved, High-quality Ovine Reference Genome Assembly

Abstract Sheep are an important agricultural species used for both food and fiber in the United States and globally. A high-quality reference genome enhances the ability to discover genetic and biological mechanisms influencing important traits, such as meat and wool quality. The rapid advances in genome assembly algorithms and emergence of increasingly long sequence read length provide the opportunity for an improved de novo assembly of the sheep reference genome. Tissue was collected postmortem from an adult Rambouillet ewe selected by USDA-ARS for the Ovine Functional Annotation of Animal Genomes project. Short-read (55x coverage), long-read PacBio (75x coverage), and Hi-C data from this ewe were retrieved from public databases. We generated an additional 50x coverage of Oxford Nanopore data and assembled the combined long-read data with canu v1.9. The assembled contigs were polished with Nanopolish v0.12.5 and scaffolded using Hi-C data with Salsa v2.2. Gaps were filled with PBsuite v15.8.24 and polished with Nanopolish v0.12.5 followed by removal of duplicate contigs with PurgeDups v1.0.1. Chromosomes were oriented by identifying centromeres and telomeres with RepeatMasker v4.1.1, indicating a need to reverse the orientation of chromosome 11 relative to Oar_rambouillet_v1.0. Final polishing was performed with two rounds of a pipeline which consisted of freebayes v1.3.1 to call variants, Merfin to validate them, and BCFtools to generate the consensus fasta. The ARS-UI_Ramb_v2.0 assembly has improved continuity (contig N50 of 43.19 Mb) with a 19-fold and 38-fold decrease in the number of scaffolds compared with Oar_rambouillet_v1.0 and Oar_v4.0. ARS-UI_Ramb_v2.0 has greater per-base accuracy and fewer insertions and deletions identified from mapped RNA sequence than previous assemblies. This significantly improved reference assembly, public at NCBI GenBank under accession number GCA_016772045, will optimize the functional annotation of the sheep genome and facilitate improved mapping accuracy of genetic variant and expression data for traits relevant the sheep industry.

Novel direct growth of ZIF-67 derived Co3O4 and N-doped carbon composites on carbon cloth as supercapacitor electrodes

Zeolitic imidazolate framework-67 (ZIF67) derivatives are considered as promising active materials for energy storage owing to the possible formation of cobalt oxide and N-doped graphite. Cobalt oxide has multiple redox states for generating redox reactions for charge storage, while N-doped graphite can provide high electrical conductivity for charge transfer. In this study, it is the first time to synthesize binder-free electrodes composed of cobalt oxide and N-doped graphite derived from ZIF67 on carbon cloth (CC) for supercapacitor (SC). Successive oxidation and carbonization along with additional coverage of ZIF67 derivatives are applied to synthesize ZIF67 derivatives composed of cobalt oxide, N-doped graphite and cobalt oxide/N-doped graphite composites with different layer compositions. The highest specific capacitance (CF) of 90.0F/g at 20 mV/s is obtained for the oxidized ZIF67/carbonized ZIF67/carbon cloth (O67/C67/CC) electrode, due to the large surface area and high electrical conductivity benefitted from preferable morphology and growing sequence of Co3O4 and N-doped graphite. The symmetric SC composed of O67/C67/CC electrodes shows the maximum energy density of 2.53 Wh/kg at the power density of 50 W/kg. Cycling stability with CF retention of 70% and Coulombic efficiency of 65% after 6000 times repeatedly charge/discharge process is also obtained for this symmetric SC.

The added value of satellite observations of methane forunderstanding the contemporary methane budget

Surface observations have recorded large and incompletely understood changes to atmospheric methane (CH4) this century. However, their ability to reveal the responsible surface sources and sinks is limited by their geographical distribution, which is biased towards the northern midlatitudes. Data from Earth-orbiting satellites designed specifically to measure atmospheric CH4 have been available since 2009 with the launch of the Japanese Greenhouse gases Observing SATellite (GOSAT). We assess the added value of GOSAT to data collected by the US National Oceanic and Atmospheric Administration (NOAA), which have been the lynchpin for knowledge about atmospheric CH4 since the 1980s. To achieve that we use the GEOS-Chem atmospheric chemistry transport model and an inverse method to infer a posteriori flux estimates from the NOAA and GOSAT data using common a priori emission inventories. We find the main benefit of GOSAT data is from its additional coverage over the tropics where we report large increases since the 2014/2016 El Niño, driven by biomass burning, biogenic emissions and energy production. We use data from the European TROPOspheric Monitoring Instrument to show how better spatial coverage and resolution measurements allow us to quantify previously unattainable diffuse sources of CH4, thereby opening up a new research frontier.This article is part of a discussion meeting issue ‘Rising methane: is warming feeding warming? (part 1)’.

Analisis Ketersediaan Air Waduk Gadjah Mungkur dengan Adanya Pembangunan Intake dan Jaringan Pipa Trasmisi Wosusokas

Gadjah Mungkur Reservoir is one of the multipurpose reservoirs built by damming the flow of the Bengawan Solo River. The Gadjah Mungkur Reservoir has existed for a long time with the main purpose of controlling flooding, but over time, this reservoir is also useful as a hydropower plant, irrigation, and also to meet the raw water needs of Wonogiri City. The potential of the Gadjah Mungkur Reservoir itself in meeting the needs of drinking water is an essential function in the people’s life around the reservoir. Therefore, the expansion of the scope of providing drinking water can be used to develop the potential of the Gadjah Mungkur Reservoir. This coverage expansion was realized through the construction of the Wosusokas intake and transmission pipeline network to drinking water receiving areas, namely Wonogiri, Sukoharjo, Solo, and Karanganyar, with service coverage of drinking water access resepectively ±78,38, ±70,97%, ±81,85%, and ±70,97%. This study aims to analyze the water availability of the Gadjah Mungkur Reservoir with additional coverage for drinking water services. The method used is a simple reservoir routing calculation by processing secondary data obtained in the form of inflow, outflow, and reservoir storage characteristics. The results of the analysis show that elevation variations with the construction of the Wosusokas intake and transmission pipeline have an annual average of +132,56 m in 2017, with a reservoir storage volume reaching 216,27 MCM and +134,29 m in 2018, with a reservoir storage volume reaching 284,92 MCM. However, there are some elevation values that exceed +138,0 m, namely at February to March 2018. However, overall, the results of the analysis show that the Wosusokas construction can increase the potential utilization of the Gadjah Mungkur Reservoir without endangering the reservoir with water level fluctuations that can still be controlled and in accordance with the regulation of reservoir water level.

Comparative pharmacoeconomic analysis of biologicals used for metastatic urothelial carcinoma

Objective: to determine the economic and clinical consequences of using atezolizumab in metastatic urothelial cancer compared with pembrolizumab and nivolumab.Materials and methods. An assessment of the effectiveness and safety of medicines for urothelial cancer was carried out on the basis of a systematic search and review of clinical studies and an analysis of direct medical costs for medicines from public procurement in Moscow in 2019-2020 and information from official instructions for medical use.Results. Systematic search identifies 4, 4 and 7 clinical trials of nivolumab, pembrolizumab and atezolizumab, respectively, as well as 2 meta-analyses. The obtained data on the efficacy and safety did not allow us to identify greater or lesser effective options. Calculation of cost of three months therapy revealed that the cost of atezolizumab (935 thousand rubles) is 7 % lower vs. pembrolizumab (1 million rubles) and 18 % lower vs. nivolumab (1,136 million rubles). Thus, when using atezolizumab instead of pembrolizumab or atezolizumab, budget savings may occur, or allowing additional therapy to be provided to every 14th or every 6th patient, respectively within fixed budget.Conclusion. The use of atezolizumab in metastatic urothelial cancer led to budget savings or the possibility of additional treatment coverage with immuno-oncological therapy.

Optimizing tropical forest bird surveys using passive acoustic monitoring and high temporal resolution sampling

AbstractEstimation of avian biodiversity is a cornerstone measure of ecosystem condition. Surveys conducted using autonomous recorders are often more efficient at estimating diversity than traditional point‐count surveys. However, there is limited research into the optimal temporal resolution for sampling—the trade‐off between the number of samples and sample duration when sampling a survey window with a fixed survey effort—despite autonomous recorders allowing easy repeat sampling compared to traditional survey methods. We assess whether the additional temporal coverage from high temporal resolution (HTR) sampling, consisting of 240 15‐s samples spread randomly across a survey window detects higher alpha and gamma diversity than low temporal resolution (LTR) sampling of four 15‐min samples at the same locations. We do so using an acoustic dataset collected from 29 locations in a region of very high avian biodiversity—the eastern Brazilian Amazon. We find HTR sampling outperforms LTR sampling in every metric considered, with HTR sampling predicted to detect approximately 50% higher alpha diversity, and 10% higher gamma diversity. This effect is primarily driven by increased coverage of variation in detectability across the morning, with the earliest period containing a distinct community that is often under sampled using LTR sampling. LTR sampling produced almost four times as many false absences for species presence. Additionally, LTR sampling incorrectly found 70 species (34%) at only a single forest type when they were in fact present in multiple forest types, while the use of HTR sampling reduced this to just two species (0.9%). When considering multiple independent detections of species, HTR sampling detected three times more uncommon species than LTR sampling. We conclude that high temporal resolution sampling of passive‐acoustic monitoring‐based surveys should be considered the primary method for estimating the species richness of bird communities in tropical forests.

Oxyresveratrol: Sources, Productions, Biological Activities, Pharmacokinetics, and Delivery Systems.

Oxyresveratrol has recently attracted much research attention due to its simple chemical structure and diverse therapeutic potentials. Previous reviews describe the chemistry and biological activities of this phytoalexin, but additional coverage and greater accessibility are still needed. The current review provides a more comprehensive summary, covering research from 1955 to the present year. Oxyresveratrol occurs in both gymnosperms and angiosperms. However, it has never been reported in plants in the subclass Sympetalae, and this point might be of both chemotaxonomic and biosynthetic importance. Oxyresveratrol can be easily obtained from plant materials by conventional methods, and several systems for both qualitative and quantitative analysis of oxyresveratrol contents in plant materials and plant products are available. Oxyresveratrol possesses diverse biological and pharmacological activities such as the inhibition of tyrosinase and melanogenesis, antioxidant and anti-inflammatory activities, and protective effects against neurological disorders and digestive ailments. However, the unfavorable pharmacokinetic properties of oxyresveratrol, including low water solubility and poor oral availability and stability, have posed challenges to its development as a useful therapeutic agent. Recently, several delivery systems have emerged, with promising outcomes that may improve chances for the clinical study of oxyresveratrol.